LOCUS       DACDID010000001        73614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDID010000001 DACDID010000000
VERSION     DACDID010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.001"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..96
                     /locus_tag="IV236_000001"
     CDS             <1..96
                     /locus_tag="IV236_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8770228.1"
                     /translation="YCPKCKNETLINVQKMNIITIKEPDAKTQSR"
     gene            complement(154..507)
                     /locus_tag="IV236_000002"
     CDS             complement(154..507)
                     /locus_tag="IV236_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770229.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1038..1460
                     /locus_tag="IV236_000003"
     CDS             1038..1460
                     /locus_tag="IV236_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8770230.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1457..1687
                     /locus_tag="IV236_000004"
     CDS             1457..1687
                     /locus_tag="IV236_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8770231.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2185..2385
                     /locus_tag="IV236_000005"
     CDS             2185..2385
                     /locus_tag="IV236_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8770232.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2412..3605
                     /locus_tag="IV236_000006"
     CDS             2412..3605
                     /locus_tag="IV236_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8770233.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3673..5238)
                     /gene="guaA"
                     /locus_tag="IV236_000007"
     CDS             complement(3673..5238)
                     /gene="guaA"
                     /locus_tag="IV236_000007"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8770234.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5297..6220)
                     /locus_tag="IV236_000008"
     CDS             complement(5297..6220)
                     /locus_tag="IV236_000008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8770235.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6416..7021
                     /locus_tag="IV236_000009"
     CDS             6416..7021
                     /locus_tag="IV236_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8770236.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7329..8345
                     /locus_tag="IV236_000010"
     CDS             7329..8345
                     /locus_tag="IV236_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8770237.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8486..9787
                     /locus_tag="IV236_000011"
     CDS             8486..9787
                     /locus_tag="IV236_000011"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8770238.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9833..10495
                     /gene="deoC"
                     /locus_tag="IV236_000012"
     CDS             9833..10495
                     /gene="deoC"
                     /locus_tag="IV236_000012"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8770239.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10516..10914
                     /locus_tag="IV236_000013"
     CDS             10516..10914
                     /locus_tag="IV236_000013"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8770240.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10980..12062
                     /locus_tag="IV236_000014"
     CDS             10980..12062
                     /locus_tag="IV236_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770241.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12274..13836
                     /locus_tag="IV236_000015"
     CDS             12274..13836
                     /locus_tag="IV236_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770242.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13829..14962
                     /locus_tag="IV236_000016"
     CDS             13829..14962
                     /locus_tag="IV236_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770243.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14962..15918
                     /locus_tag="IV236_000017"
     CDS             14962..15918
                     /locus_tag="IV236_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770244.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16017..17183
                     /gene="deoB"
                     /locus_tag="IV236_000018"
     CDS             16017..17183
                     /gene="deoB"
                     /locus_tag="IV236_000018"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8770245.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17207..18025
                     /locus_tag="IV236_000019"
     CDS             17207..18025
                     /locus_tag="IV236_000019"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8770246.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18042..18749
                     /gene="deoD"
                     /locus_tag="IV236_000020"
     CDS             18042..18749
                     /gene="deoD"
                     /locus_tag="IV236_000020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8770247.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19047..19733)
                     /gene="gpmA"
                     /locus_tag="IV236_000021"
     CDS             complement(19047..19733)
                     /gene="gpmA"
                     /locus_tag="IV236_000021"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8770248.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(19905..20486)
                     /locus_tag="IV236_000022"
     CDS             complement(19905..20486)
                     /locus_tag="IV236_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8770249.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20619..21302)
                     /gene="rpiA"
                     /locus_tag="IV236_000023"
     CDS             complement(20619..21302)
                     /gene="rpiA"
                     /locus_tag="IV236_000023"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8770250.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21621..22034
                     /gene="rpsL"
                     /locus_tag="IV236_000024"
     CDS             21621..22034
                     /gene="rpsL"
                     /locus_tag="IV236_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8770251.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22118..22588
                     /gene="rpsG"
                     /locus_tag="IV236_000025"
     CDS             22118..22588
                     /gene="rpsG"
                     /locus_tag="IV236_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8770252.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22682..24769
                     /gene="fusA"
                     /locus_tag="IV236_000026"
     CDS             22682..24769
                     /gene="fusA"
                     /locus_tag="IV236_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8770253.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            24941..26128
                     /gene="tuf"
                     /locus_tag="IV236_000027"
     CDS             24941..26128
                     /gene="tuf"
                     /locus_tag="IV236_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8770254.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            26535..26843
                     /gene="rpsJ"
                     /locus_tag="IV236_000028"
     CDS             26535..26843
                     /gene="rpsJ"
                     /locus_tag="IV236_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8770255.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            26874..27503
                     /gene="rplC"
                     /locus_tag="IV236_000029"
     CDS             26874..27503
                     /gene="rplC"
                     /locus_tag="IV236_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8770256.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            27531..28154
                     /gene="rplD"
                     /locus_tag="IV236_000030"
     CDS             27531..28154
                     /gene="rplD"
                     /locus_tag="IV236_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8770257.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28154..28444
                     /gene="rplW"
                     /locus_tag="IV236_000031"
     CDS             28154..28444
                     /gene="rplW"
                     /locus_tag="IV236_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8770258.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            28483..29316
                     /gene="rplB"
                     /locus_tag="IV236_000032"
     CDS             28483..29316
                     /gene="rplB"
                     /locus_tag="IV236_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8770259.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29359..29637
                     /gene="rpsS"
                     /locus_tag="IV236_000033"
     CDS             29359..29637
                     /gene="rpsS"
                     /locus_tag="IV236_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8770260.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            29660..30007
                     /gene="rplV"
                     /locus_tag="IV236_000034"
     CDS             29660..30007
                     /gene="rplV"
                     /locus_tag="IV236_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8770261.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30021..30677
                     /gene="rpsC"
                     /locus_tag="IV236_000035"
     CDS             30021..30677
                     /gene="rpsC"
                     /locus_tag="IV236_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8770262.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            30680..31114
                     /gene="rplP"
                     /locus_tag="IV236_000036"
     CDS             30680..31114
                     /gene="rplP"
                     /locus_tag="IV236_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8770263.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31104..31292
                     /gene="rpmC"
                     /locus_tag="IV236_000037"
     CDS             31104..31292
                     /gene="rpmC"
                     /locus_tag="IV236_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8770264.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31317..31583
                     /gene="rpsQ"
                     /locus_tag="IV236_000038"
     CDS             31317..31583
                     /gene="rpsQ"
                     /locus_tag="IV236_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8770265.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            31641..32009
                     /gene="rplN"
                     /locus_tag="IV236_000039"
     CDS             31641..32009
                     /gene="rplN"
                     /locus_tag="IV236_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8770266.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32046..32354
                     /gene="rplX"
                     /locus_tag="IV236_000040"
     CDS             32046..32354
                     /gene="rplX"
                     /locus_tag="IV236_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8770267.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32382..32921
                     /gene="rplE"
                     /locus_tag="IV236_000041"
     CDS             32382..32921
                     /gene="rplE"
                     /locus_tag="IV236_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8770268.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            32940..33125
                     /locus_tag="IV236_000042"
     CDS             32940..33125
                     /locus_tag="IV236_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8770269.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33161..33559
                     /gene="rpsH"
                     /locus_tag="IV236_000043"
     CDS             33161..33559
                     /gene="rpsH"
                     /locus_tag="IV236_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8770270.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            33591..34127
                     /gene="rplF"
                     /locus_tag="IV236_000044"
     CDS             33591..34127
                     /gene="rplF"
                     /locus_tag="IV236_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8770271.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34288..34644
                     /gene="rplR"
                     /locus_tag="IV236_000045"
     CDS             34288..34644
                     /gene="rplR"
                     /locus_tag="IV236_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8770272.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            34665..35165
                     /gene="rpsE"
                     /locus_tag="IV236_000046"
     CDS             34665..35165
                     /gene="rpsE"
                     /locus_tag="IV236_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8770273.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35180..35359
                     /gene="rpmD"
                     /locus_tag="IV236_000047"
     CDS             35180..35359
                     /gene="rpmD"
                     /locus_tag="IV236_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8770274.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            35403..35843
                     /gene="rplO"
                     /locus_tag="IV236_000048"
     CDS             35403..35843
                     /gene="rplO"
                     /locus_tag="IV236_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8770275.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35843..37138
                     /gene="secY"
                     /locus_tag="IV236_000049"
     CDS             35843..37138
                     /gene="secY"
                     /locus_tag="IV236_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8770276.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37200..37847
                     /locus_tag="IV236_000050"
     CDS             37200..37847
                     /locus_tag="IV236_000050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8770277.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38038..38256
                     /gene="infA"
                     /locus_tag="IV236_000051"
     CDS             38038..38256
                     /gene="infA"
                     /locus_tag="IV236_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8770278.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38289..38405
                     /gene="rpmJ"
                     /locus_tag="IV236_000052"
     CDS             38289..38405
                     /gene="rpmJ"
                     /locus_tag="IV236_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8770279.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            38423..38788
                     /gene="rpsM"
                     /locus_tag="IV236_000053"
     CDS             38423..38788
                     /gene="rpsM"
                     /locus_tag="IV236_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8770280.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            38816..39205
                     /gene="rpsK"
                     /locus_tag="IV236_000054"
     CDS             38816..39205
                     /gene="rpsK"
                     /locus_tag="IV236_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8770281.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39285..40223
                     /locus_tag="IV236_000055"
     CDS             39285..40223
                     /locus_tag="IV236_000055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8770282.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40251..40631
                     /gene="rplQ"
                     /locus_tag="IV236_000056"
     CDS             40251..40631
                     /gene="rplQ"
                     /locus_tag="IV236_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8770283.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            40903..41742
                     /locus_tag="IV236_000057"
     CDS             40903..41742
                     /locus_tag="IV236_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8770284.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            41718..42587
                     /locus_tag="IV236_000058"
     CDS             41718..42587
                     /locus_tag="IV236_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8770285.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            42577..43374
                     /locus_tag="IV236_000059"
     CDS             42577..43374
                     /locus_tag="IV236_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8770286.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            43577..44323
                     /gene="truA"
                     /locus_tag="IV236_000060"
     CDS             43577..44323
                     /gene="truA"
                     /locus_tag="IV236_000060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8770287.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(44656..47325)
                     /locus_tag="IV236_000061"
     CDS             complement(44656..47325)
                     /locus_tag="IV236_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8770288.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(47563..47751)
                     /locus_tag="IV236_000062"
     CDS             complement(47563..47751)
                     /locus_tag="IV236_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8770289.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48021..48383
                     /locus_tag="IV236_000063"
     CDS             48021..48383
                     /locus_tag="IV236_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8770290.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            48435..50117
                     /locus_tag="IV236_000064"
     CDS             48435..50117
                     /locus_tag="IV236_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8770291.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50234..52270
                     /gene="recG"
                     /locus_tag="IV236_000065"
     CDS             50234..52270
                     /gene="recG"
                     /locus_tag="IV236_000065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8770292.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52319..53320
                     /gene="plsX"
                     /locus_tag="IV236_000066"
     CDS             52319..53320
                     /gene="plsX"
                     /locus_tag="IV236_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8770293.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            53442..53684
                     /gene="acpP"
                     /locus_tag="IV236_000067"
     CDS             53442..53684
                     /gene="acpP"
                     /locus_tag="IV236_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8770294.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            53952..54956
                     /locus_tag="IV236_000068"
     CDS             53952..54956
                     /locus_tag="IV236_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770295.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            54957..55901
                     /locus_tag="IV236_000069"
     CDS             54957..55901
                     /locus_tag="IV236_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770296.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            55901..56863
                     /locus_tag="IV236_000070"
     CDS             55901..56863
                     /locus_tag="IV236_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770297.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            56890..57804
                     /locus_tag="IV236_000071"
     CDS             56890..57804
                     /locus_tag="IV236_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770298.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            57830..59611
                     /locus_tag="IV236_000072"
     CDS             57830..59611
                     /locus_tag="IV236_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770299.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            59959..60645
                     /locus_tag="IV236_000073"
     CDS             59959..60645
                     /locus_tag="IV236_000073"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8770300.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            60665..64246
                     /gene="smc"
                     /locus_tag="IV236_000074"
     CDS             60665..64246
                     /gene="smc"
                     /locus_tag="IV236_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8770301.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64255..65064
                     /locus_tag="IV236_000075"
     CDS             64255..65064
                     /locus_tag="IV236_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8770302.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65075..66073
                     /gene="ftsY"
                     /locus_tag="IV236_000076"
     CDS             65075..66073
                     /gene="ftsY"
                     /locus_tag="IV236_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8770303.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66260..68527
                     /gene="gshAB"
                     /locus_tag="IV236_000077"
     CDS             66260..68527
                     /gene="gshAB"
                     /locus_tag="IV236_000077"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8770304.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(68616..69068)
                     /locus_tag="IV236_000078"
     CDS             complement(68616..69068)
                     /locus_tag="IV236_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8770305.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69218..70168
                     /locus_tag="IV236_000079"
     CDS             69218..70168
                     /locus_tag="IV236_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8770306.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70161..71120
                     /locus_tag="IV236_000080"
     CDS             70161..71120
                     /locus_tag="IV236_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8770307.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71117..71872
                     /locus_tag="IV236_000081"
     CDS             71117..71872
                     /locus_tag="IV236_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770308.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            71895..72848
                     /locus_tag="IV236_000082"
     CDS             71895..72848
                     /locus_tag="IV236_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770309.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        23967 a        13362 c        15846 g        20439 t
ORIGIN      
        1 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
       61 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
      121 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
      181 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
      241 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
      301 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
      361 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
      421 ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa
      481 atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta
      541 tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac
      601 tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta
      661 cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta
      721 atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc
      781 ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag
      841 attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt
      901 tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca aatcccccgt
      961 tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca
     1021 gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg
     1081 actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca
     1141 gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc aactcttttt
     1201 ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga ttaagaatca
     1261 atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca
     1321 ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag ttactaaatt
     1381 tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt aaagaattca
     1441 tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc tcattgtaaa
     1501 ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc gagggtatac
     1561 ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata tggttattga
     1621 tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat cgttcgaaat
     1681 taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg
     1741 aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag gctaacaagc
     1801 caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag aaaagagctg
     1861 atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt aattcgaaag
     1921 taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac tcccgtatag
     1981 ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga gaaccatgca
     2041 gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat tcttctcttg
     2101 tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga gttttcctag
     2161 cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt agttgagcta
     2221 ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt gatgattaca
     2281 aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga agctgttgaa
     2341 gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt gcacagtaag
     2401 gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc tattattttc
     2461 gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt agtggattca
     2521 ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa ccagaagaat
     2581 taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt ttccttccat
     2641 ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact attaagaagc
     2701 atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat gtgcaaaatt
     2761 ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt caaggactat
     2821 tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat ccatctaaga
     2881 ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa gaagaatttg
     2941 aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta tttatctctc
     3001 tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt caatgggaag
     3061 atattgattt tgactcagga gtactaacca tcaacaaaac actctattac aagaatcaat
     3121 cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata
     3181 aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta attcagacag
     3241 actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca tatgctattg
     3301 aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta cggcattctc
     3361 atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat cgtctaggac
     3421 acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac agtaatttcg
     3481 aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa aattatgata
     3541 cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg aaaaaagtgc
     3601 aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag aggatttcta
     3661 gctctcctac aactattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
     3721 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
     3781 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
     3841 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
     3901 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
     3961 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
     4021 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
     4081 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
     4141 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
     4201 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
     4261 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
     4321 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
     4381 tgcatcgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
     4441 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
     4501 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
     4561 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
     4621 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
     4681 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
     4741 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
     4801 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
     4861 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
     4921 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
     4981 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
     5041 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
     5101 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
     5161 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
     5221 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
     5281 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
     5341 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
     5401 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
     5461 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
     5521 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
     5581 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
     5641 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
     5701 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
     5761 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
     5821 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
     5881 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
     5941 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
     6001 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
     6061 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
     6121 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
     6181 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
     6241 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
     6301 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
     6361 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
     6421 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
     6481 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
     6541 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
     6601 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
     6661 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
     6721 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
     6781 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
     6841 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
     6901 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
     6961 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
     7021 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
     7081 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
     7141 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
     7201 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
     7261 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
     7321 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
     7381 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
     7441 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
     7501 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
     7561 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
     7621 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
     7681 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
     7741 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
     7801 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
     7861 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
     7921 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
     7981 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
     8041 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
     8101 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
     8161 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
     8221 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
     8281 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
     8341 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
     8401 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
     8461 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
     8521 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
     8581 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
     8641 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
     8701 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
     8761 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
     8821 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
     8881 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
     8941 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
     9001 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
     9061 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
     9121 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
     9181 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
     9241 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
     9301 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
     9361 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
     9421 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
     9481 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
     9541 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
     9601 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
     9661 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
     9721 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
     9781 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
     9841 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
     9901 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
     9961 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    10021 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    10081 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    10141 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    10201 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    10261 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    10321 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    10381 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    10441 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    10501 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    10561 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    10621 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    10681 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    10741 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    10801 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    10861 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    10921 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    10981 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    11041 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    11101 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    11161 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    11221 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    11281 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    11341 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    11401 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    11461 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    11521 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    11581 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    11641 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    11701 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    11761 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    11821 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    11881 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    11941 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    12001 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    12061 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    12121 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    12181 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    12241 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    12301 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    12361 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    12421 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    12481 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    12541 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    12601 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    12661 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    12721 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    12781 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    12841 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    12901 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    12961 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    13021 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    13081 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    13141 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    13201 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    13261 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    13321 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    13381 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    13441 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    13501 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    13561 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    13621 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    13681 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    13741 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    13801 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    13861 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    13921 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    13981 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    14041 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    14101 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    14161 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    14221 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    14281 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    14341 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    14401 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    14461 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    14521 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    14581 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    14641 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    14701 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    14761 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    14821 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    14881 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    14941 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    15001 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    15061 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    15121 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    15181 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    15241 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    15301 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    15361 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    15421 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    15481 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    15541 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    15601 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    15661 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    15721 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    15781 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    15841 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    15901 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    15961 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    16021 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    16081 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    16141 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    16201 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    16261 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    16321 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    16381 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    16441 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    16501 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    16561 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    16621 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    16681 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    16741 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    16801 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    16861 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    16921 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    16981 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    17041 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    17101 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    17161 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    17221 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    17281 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    17341 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    17401 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    17461 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    17521 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    17581 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    17641 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    17701 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    17761 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    17821 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    17881 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    17941 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    18001 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    18061 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    18121 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    18181 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    18241 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    18301 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    18361 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    18421 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    18481 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    18541 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    18601 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    18661 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    18721 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    18781 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    18841 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    18901 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    18961 gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg
    19021 tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca
    19081 tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg
    19141 tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact
    19201 aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg
    19261 cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg
    19321 taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta
    19381 tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt
    19441 ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt
    19501 ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt
    19561 aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct
    19621 tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg
    19681 ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc
    19741 tctcctttta agataggttt atatttctga aattttcagt tctgttttat tgtacaattt
    19801 tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat
    19861 ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc
    19921 tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat
    19981 tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg
    20041 atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct
    20101 aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt
    20161 aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag
    20221 tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct
    20281 aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac
    20341 tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct
    20401 tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt
    20461 gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg
    20521 atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc
    20581 cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca
    20641 agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc
    20701 ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg
    20761 atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg
    20821 ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt
    20881 tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat
    20941 ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa
    21001 agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg
    21061 tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt
    21121 tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca
    21181 cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct
    21241 acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc
    21301 atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga
    21361 tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc
    21421 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    21481 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    21541 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    21601 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    21661 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    21721 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    21781 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    21841 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    21901 tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg
    21961 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    22021 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    22081 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    22141 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    22201 ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga
    22261 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    22321 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    22381 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    22441 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    22501 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    22561 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    22621 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    22681 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    22741 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    22801 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    22861 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    22921 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    22981 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    23041 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    23101 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    23161 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    23221 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    23281 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    23341 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    23401 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    23461 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    23521 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    23581 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    23641 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    23701 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    23761 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    23821 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    23881 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    23941 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    24001 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    24061 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    24121 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    24181 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    24241 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    24301 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    24361 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    24421 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    24481 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    24541 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    24601 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    24661 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    24721 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac
    24781 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    24841 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    24901 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    24961 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    25021 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    25081 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    25141 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    25201 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    25261 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    25321 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    25381 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    25441 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    25501 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    25561 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    25621 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    25681 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    25741 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    25801 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    25861 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    25921 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    25981 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    26041 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    26101 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    26161 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    26221 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
    26281 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
    26341 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
    26401 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
    26461 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
    26521 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
    26581 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
    26641 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
    26701 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
    26761 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
    26821 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
    26881 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
    26941 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
    27001 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
    27061 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
    27121 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
    27181 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
    27241 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
    27301 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
    27361 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
    27421 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
    27481 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
    27541 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
    27601 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
    27661 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
    27721 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
    27781 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
    27841 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
    27901 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
    27961 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
    28021 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
    28081 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
    28141 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
    28201 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
    28261 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
    28321 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
    28381 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
    28441 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
    28501 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
    28561 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
    28621 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
    28681 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
    28741 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
    28801 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
    28861 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
    28921 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
    28981 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
    29041 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
    29101 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
    29161 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
    29221 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
    29281 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
    29341 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
    29401 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
    29461 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
    29521 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
    29581 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
    29641 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
    29701 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
    29761 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
    29821 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
    29881 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
    29941 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
    30001 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
    30061 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
    30121 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
    30181 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
    30241 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
    30301 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
    30361 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
    30421 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
    30481 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
    30541 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
    30601 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
    30661 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
    30721 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
    30781 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
    30841 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
    30901 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
    30961 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
    31021 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
    31081 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
    31141 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
    31201 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
    31261 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
    31321 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
    31381 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
    31441 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
    31501 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
    31561 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
    31621 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
    31681 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
    31741 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
    31801 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
    31861 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
    31921 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
    31981 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
    32041 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
    32101 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
    32161 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
    32221 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
    32281 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
    32341 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
    32401 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
    32461 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
    32521 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
    32581 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
    32641 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
    32701 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
    32761 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
    32821 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
    32881 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
    32941 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
    33001 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
    33061 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
    33121 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
    33181 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
    33241 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
    33301 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
    33361 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
    33421 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
    33481 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
    33541 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
    33601 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
    33661 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
    33721 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
    33781 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
    33841 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
    33901 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
    33961 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
    34021 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
    34081 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
    34141 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
    34201 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
    34261 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
    34321 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
    34381 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
    34441 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
    34501 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
    34561 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
    34621 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
    34681 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
    34741 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
    34801 tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
    34861 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
    34921 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
    34981 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
    35041 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
    35101 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
    35161 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
    35221 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
    35281 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
    35341 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
    35401 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
    35461 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
    35521 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
    35581 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
    35641 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
    35701 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
    35761 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
    35821 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
    35881 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
    35941 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
    36001 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
    36061 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
    36121 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
    36181 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    36241 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    36301 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    36361 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    36421 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    36481 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    36541 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    36601 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    36661 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    36721 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    36781 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    36841 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    36901 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    36961 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    37021 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    37081 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    37141 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    37201 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    37261 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    37321 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    37381 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    37441 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    37501 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    37561 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    37621 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    37681 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    37741 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    37801 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    37861 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    37921 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    37981 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    38041 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    38101 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    38161 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    38221 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    38281 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    38341 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    38401 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    38461 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    38521 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    38581 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    38641 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    38701 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    38761 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    38821 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    38881 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    38941 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    39001 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    39061 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    39121 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    39181 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    39241 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    39301 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    39361 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    39421 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    39481 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    39541 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    39601 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    39661 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    39721 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    39781 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    39841 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    39901 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    39961 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    40021 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    40081 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    40141 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    40201 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    40261 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    40321 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    40381 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    40441 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    40501 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    40561 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    40621 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    40681 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    40741 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    40801 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    40861 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    40921 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    40981 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    41041 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    41101 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatgatt
    41161 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    41221 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    41281 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    41341 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    41401 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    41461 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    41521 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    41581 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    41641 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    41701 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    41761 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    41821 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    41881 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    41941 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    42001 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    42061 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    42121 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    42181 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    42241 gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    42301 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    42361 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    42421 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    42481 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    42541 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    42601 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    42661 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    42721 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    42781 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    42841 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    42901 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    42961 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    43021 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    43081 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    43141 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    43201 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    43261 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    43321 gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc
    43381 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg
    43441 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    43501 gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac cttttaggaa
    43561 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg gaacaaattt
    43621 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    43681 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    43741 agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    43801 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    43861 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    43921 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    43981 acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    44041 ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct acgaggctaa
    44101 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    44161 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    44221 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    44281 acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta tgatagaagt
    44341 ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat tttgcaaaat
    44401 ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag gtcgtgagcc
    44461 tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca tctttgatga
    44521 atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt
    44581 ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac agtctgaaca
    44641 ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc
    44701 tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc agacataaag
    44761 aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa
    44821 gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga atgcgtcttt
    44881 gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt tgtcataagg
    44941 aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag cttttctgcc
    45001 agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt gataggctac
    45061 aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc cataaaaact
    45121 tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt attgaccaga
    45181 aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac tagaactttc
    45241 taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg caggcacatg
    45301 gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag tgacgccagt
    45361 cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat agaatgtaaa
    45421 atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg tcaaattatc
    45481 tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta gtggcaatgc
    45541 attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga agccatattt
    45601 actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc catccatcag
    45661 caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga aacctaattt
    45721 atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga taccactgta
    45781 accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc ggtaaaacga
    45841 gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag gatcagtata
    45901 taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac tgttcgtatt
    45961 gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat tgaatgaaag
    46021 ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa
    46081 tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc cccatgccac
    46141 cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat cgtcttgttc
    46201 tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca gataggcata
    46261 acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca atggaacaag
    46321 ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat ttttcaacgg
    46381 tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac ctgcgtaaac
    46441 gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt tcatgattag
    46501 atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt
    46561 tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa
    46621 agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg tcaatagacg
    46681 ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa aactggataa
    46741 aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca taacacgatg
    46801 aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga tgatctccga
    46861 atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat gcccccagcg
    46921 aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc cgaccttaag
    46981 caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga acaccagtgg
    47041 cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc ctagagaagc
    47101 attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc tggcatgaaa
    47161 attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc caggatagat
    47221 tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac tagctgctgc
    47281 gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta ttttccacca
    47341 aatttctatg aagttgtatt cagtttatca tatccttgtt tttctctgct taaaaagagt
    47401 ctagttttca caaaaaaata aaatttagaa taaataactt cataatttgt tcgtataaca
    47461 aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaaaaat ttttattaaa
    47521 taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc gttcaacttt
    47581 tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt cgaccataac
    47641 gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag cgtgtgaacg
    47701 gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca tggtaatttc
    47761 ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact aacatatcat
    47821 atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga gaagaataat
    47881 tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact ttgggtaact
    47941 gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg gagtttttta
    48001 cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga accattgaaa
    48061 ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg ttcggtgttg
    48121 taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg cgtaaagaaa
    48181 attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta gatgtttata
    48241 cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa gaaaaagtga
    48301 aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag
    48361 gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag tcgatctgaa
    48421 ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag gaaatggtcc
    48481 aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta aatgtattcc
    48541 ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt ggagctaagg
    48601 cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt tcaaaaggat
    48661 tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt cgtggatttt
    48721 ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca gccttcactc
    48781 atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg attttgacag
    48841 tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat gatgtcgttg
    48901 aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact ccagacttgc
    48961 ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt
    49021 atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca tatgaacctt
    49081 ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa
    49141 tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa atcggagaag
    49201 gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta gatggtatag
    49261 gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg catacagaaa
    49321 acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa atcaaagtcg
    49381 acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg aacgagtttg
    49441 cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg
    49501 tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga caaacaatga
    49561 atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat aaagtgattg
    49621 tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa gtagcagata
    49681 tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct atgctttcat
    49741 tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg
    49801 ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt gtagagatca
    49861 aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac ccagatatca
    49921 cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag atcgtaacga
    49981 tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag aaactaacag
    50041 aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc tatccttatc
    50101 tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat tcattcataa
    50161 aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt gttaaaggag
    50221 ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag gagtcggaga
    50281 aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact tactgactta
    50341 ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc aggatcaaga
    50401 aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat ttggttataa
    50461 aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg tttctttttt
    50521 taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg tatacggtaa
    50581 atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc aaacaatgga
    50641 tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat tagttgatct
    50701 aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc caaacaacct
    50761 tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc attttccaaa
    50821 agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt tctttttatt
    50881 tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat
    50941 cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg aactgacaaa
    51001 tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc agcatatgca
    51061 gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga ttgcgttata
    51121 cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa tcttggcaca
    51181 acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca tcgcattgat
    51241 tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag agcaagggga
    51301 aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat ttcagaattt
    51361 aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga aagtacttcg
    51421 tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc ctagaacatt
    51481 agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc cagctggacg
    51541 tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac tggattggac
    51601 gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc
    51661 agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg aactatttgc
    51721 accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa aagaagtaat
    51781 catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg taattgaagt
    51841 aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc gatttggatt
    51901 ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt cctactgtat
    51961 cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca tgacagagac
    52021 caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt
    52081 tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga cggatggaaa
    52141 tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg attggcagat
    52201 gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg ataatcgtta
    52261 tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag tggaaacaat
    52321 gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg tagaaggcgt
    52381 ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa aagaagcaga
    52441 aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag acgaaaaaat
    52501 caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat caatggtcct
    52561 tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag ggaatacagg
    52621 cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg
    52681 cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata tgctggatct
    52741 tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt taggttcttt
    52801 ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa acaacggaac
    52861 agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc agaatgaaac
    52921 aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt
    52981 agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag gaacagcaat
    53041 gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag gaaaaatggg
    53101 cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt attccaaaca
    53161 tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg gagcaacagg
    53221 ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga cagatgtagt
    53281 tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata tctgaagcga
    53341 tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat tagccatggt
    53401 gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat
    53461 ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat aaagtgacgg
    53521 atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg gaattcgttt
    53581 tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa caaattgaaa
    53641 cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa ataaaagcaa
    53701 agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt tttgacacgt
    53761 aaaagtcatt tttctgataa ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg
    53821 ttttctatat aattattaat aattcattac acaaagataa gaataacctt tgttataaaa
    53881 cgatttattc tgtgtaatgt ccttttttat taagaaaaac ttccatttag gaagcagaga
    53941 ggggtattcg attgtccgat aaccaattat tagaagttca gaacctacat acaggattcc
    54001 gactcaaaga tgactattac gatgcagtag atgacgtttc atttacttta gacaaaaacg
    54061 agattttagc aatcgttggg gaatctgggt gcgggaaaag taccttggct actacgatta
    54121 tgggattact tgatcctaat aacacaaaaa ttacaggtga gattttatat aacgatctga
    54181 atttaatcga cctgaacgaa acgctctata ataaaatccg cggaaatgat atcggaatga
    54241 ttttccaaga tccgttgtct gcattaaatc ctctcatgag aatcgaagac cagatcaaag
    54301 aaggactttc ctatcacaca aagatgaatg cagaacagcg tcaagctcgt gcactagaat
    54361 tgctagaaca agtagggatt cctaatcctg cgagagttgg acgccaatat ccccatgaac
    54421 tatcaggcgg tatgagacaa cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa
    54481 tcgtcattgc agatgaaccg acgacagccc tagatgtaac catccaagca cagattcttg
    54541 atttactgaa agaccttcag gaagaaacaa aaacagggat catcttgatc actcatgact
    54601 taggagtcgt agcagaaatg gctgatcgag tggcagtgat gtatggcgga cagttcgttg
    54661 aagtagcagg tgtaaaagaa ctgtttgatc atcctaagca tccttataca cgctctttac
    54721 tgaactctat accacaagaa ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg
    54781 ttccttctct gaaaaacatg cctcgtaaag gttgtcggtt tgcaccacgt attccatgga
    54841 taccagcaag cgatcatgaa gaagagccta tccttcatga agtcgcaccg aaccacttgg
    54901 tacgttgctc ctgctataag catttccact ttagagacga aaaaggagag gtgtagatga
    54961 cagaattaat cacaatcaaa gacctgaaag ttcattaccc tattcgcagt ggattcttca
    55021 atcgagtaac ggatcatgta tatgctgtag atggtgtgga ttttattatc gaaaaaggaa
    55081 agacatatgg tttagtggga gagtctggct ctggaaaatc tactacggga aaagccgttg
    55141 tgggattaga aaaggtaaca gccggacaaa tcatgtatga aggaaaagat gtaacgaagc
    55201 gaagcaaccg caaaaaaatg ggttataaca aggatgtgca gatgatcttc caagattcaa
    55261 tgtccagttt gaatcctaaa aagcgagtgt tggatatcat tgctgaaccc atccgaaatt
    55321 ttgaacggtt aagcgatcaa gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg
    55381 ggatgccgga agatgcgcta tataaatatc ctcatgaatt ttcaggcgga caacgtcaac
    55441 gtctaggagt agcccgtgct gttgctacca atccaaaact gatcgtagcc gatgaacctg
    55501 tatctgctct ggatctatct gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc
    55561 aagaatacgg cctcagctat ttattcattt cacatgattt aggtgtagta aaacacatgt
    55621 gcgacaatat cgcaattatg tacaaaggtc gatttgtaga aatcggtaca agagaagaca
    55681 tttataatga cccgcgacat atctatacga aacgtctgct ttcagctatt ccgcaaatcg
    55741 atgtagaaaa tagggaaagc cacaaagaaa atcgacgaag agtagaacaa gagtatatcc
    55801 aaaaccaaaa agaatactat gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa
    55861 ctacacacaa agtggcgttg aaagatggag gtgcaagcta atgtggaaaa cgattctgcg
    55921 acgtgtttta ttgatgatcc cgcaagttat tattttaagt gttctgatct tcttattagc
    55981 ccaagcgatg ccgggggatc catttaccgg tttgatcaat ccaaatcagg atccggcagt
    56041 gatcgaacag atgcgacaag cagctggctt gaatgaccca tggtatgtcc agtacttccg
    56101 ttggatcgga aatgctttgc aaggcgattt tggtcaaagt ttcttataca aacgctctgt
    56161 tgctgacttg atcggcgaac ggatcatcaa cactttatat ctatcgatct tgacagttgt
    56221 ccttacttat ttgattgctg tgcctctggg gatgcttgct ggccggtatc aaaattcaat
    56281 cttggataag attgtcgtaa tctacaactt ctttagtttt gctgttcctt tatttatctt
    56341 cggcttgatc atgctatttg tttttggtta tcgtttaggc tggttcccaa caagtggatc
    56401 acaaacacta ggtttgcaag gtggatttat cactcaatgg atagaccgtt tgaaatatat
    56461 tctgttacca tcaattaccc aagcattttt ggcgactgcc gtcacaatcc aatatctgcg
    56521 aagtgaagta atcgattcga aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt
    56581 accgacgaat aaagtattca gtcgacatat tttccgaaat gcagcgttgc cgatggcttc
    56641 tcaaatgggg tatgaaatca cttcgttgat tgctggttct gtagttatcg aaaagatttt
    56701 tggttatccg ggagtcggaa aactatttat tgattccatc ggccaacggg actataccgt
    56761 gatcacatcc ttagtattga ttttaggaat cgcgacgcta gtggggacat tactttcaga
    56821 tatcatcatg agtatcgttg acccgcgtat tcggattcaa taaaaccaat cagaaaggag
    56881 aatggatcga tgagtgaaaa aaacaataca gcagttcaag aaagtattcc tcctatgggg
    56941 atacgaatga ttgctcgaga atttaaaaaa gataaaattg ctattttttc attaggtttg
    57001 ctagtggttt tattattagt gatctttatc ggtgcgatgc tgaccgatca agacaaagtg
    57061 atgtatgtta gtatcttcga caaatacgct gcaccaggaa cagtttcgac acaaggacaa
    57121 aagttcattc tgggagcaga tgaaggcgga cgtgatgtat taggacaatt gatcataggt
    57181 gctagaaact ctgttacgat cggatttgcg atcacgatcc ttacttcgat catcggggtt
    57241 ggattaggga tcatctctgg ctactacgga gggatcattg acaacatctt gatgcggatc
    57301 gttgatttta tcatgattct accgatcatg ctgatcatta tcgtcttcgt atcgattatt
    57361 tccagataca atgtctggtc ctttatattc atcatgagtg cattttattg ggtagctaaa
    57421 gcccgattat tccgaagcaa gacattatct gaagcaagaa gagattatgt cagtgcctca
    57481 aaaacaatgg gaaccaatga ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt
    57541 tcattaatca ttactaattt gacgatgaac tttgcggcaa acatcggtat cgagacaact
    57601 ttgactttct taggtttcgg cttgccggca aatgtaccta gtttaggtac attgatcggt
    57661 tatgcaagta acggagaagt cttagtgaat cggcaatgga tctggttacc cgcatcaatc
    57721 cttattttag ttttgatgtt gagtatcaat tatgttggac aagcattcaa gcgttctgca
    57781 gatgcgcgcc aacggttagg ctagtataga agcgaagggg gaaataggaa tgaagaaaac
    57841 gatttttgga tttgcagcgc tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg
    57901 tacagatacc tctggaaaca actctggagg caacggcaat aacaaagagg aattaacttt
    57961 tccaattgca acaaaaaata atgatgatgc aatcgaagac ggcgaattag aagctgcggt
    58021 cgcaacggac agtcagttta aaggattgtt ccagtgggag ttttaccaag attcatatga
    58081 tgcagctttt atggctcctt ctcatgaacc gctatttgca aacgatgacg atttcagaat
    58141 cacagacgac ggagctgcaa cgcttgatct agatgaagat acgaaaaaag cgacaatcaa
    58201 agtaaaagac aacgtcaaat ggtcagatgg tgaagatgta acggcggacg atgtgatttt
    58261 cccttacgaa atcattggga ataaagatta cactggtatc cgttatgacg acactttccg
    58321 taacatcgta ggtatggaag aatatcatga tggaagtgcc gacacaatct ccggaatcaa
    58381 aaaaatagac gacaaaacag ttgaaatcga atataaagaa gtaaacccaa gcatgcttca
    58441 atcgggtggt ggtatctggt catatgcagc accaaaacat acgttagaag gtgtagcaat
    58501 caaagatatg gaatcaagcg atcaagtacg taaaaatcca gtgacttttg gcccttatta
    58561 catgagtaat atcgttgcag gagaatcagt agaatacttg ccaaacgagc actactggaa
    58621 tggtacgcct aaattgaaaa agatcacgat gaaatctgta ccgatggcaa gtgcaactga
    58681 agcattgaac tctaaattat atgatatgat tttccaaatg ccaacagata cttacgatac
    58741 ctataaagaa attgctggct atacaaactt aggccgtgaa caaacttctt atacttatct
    58801 aggattcaag ttaggtaaat ggaatgcaga tacaaagagt gtggaataca atccagattc
    58861 aaaaatggct gataaatcat tacgtcaagc aatgggctat gcattagata ataatgcagt
    58921 aggtgaacgt ttctatgcag gtttgagaag caatgcgact tcattgattc ctccagtatt
    58981 ccagtcatac catgatgatg agatgaaagg attcacacaa gatctagaca aagcgaaaaa
    59041 actattagat gatgctggct acaaagatgt ggatgacgat ggtttaagag aagacaaaga
    59101 tggaaataaa ctaacaatca actttgcttc gatgtccggt ggggaaacag cgcaaccatt
    59161 ggcagattac tatatccaac aatggaaaga aattggtttg gatgtccaat acacaacagg
    59221 tcgtctgatc gaattcaatt ctttctatga taaattagaa aacgatgatc ctgaaattga
    59281 tgtttaccaa gctgcttgga atacaggaac tgatccaaat ccaagtggac tatggggacc
    59341 aaatgctgcg ttcaactaca ctcgttatga atcagaagaa aatacaaaat tgatcaacga
    59401 tatcagttca agcaaagcat ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca
    59461 acaatatgca tttgaagaag catttgcgat tccaacgttg ttccgtaatg aagtattgcc
    59521 agtcaacgac cgtgtcaaag actgggattg gtcttatgat gtaccaaacc catgggcaaa
    59581 tgtttctgtt acagcagatt cacgttcata atcactaaaa atgaccgaaa aagggcgatg
    59641 tcctttttcg gttttttgat atttttctgt ttatttctct aatattcagg tcaaggcgct
    59701 gatttcatga cttctccaat aaacggtgtt caaagtccag tttctcggta ttaagtcaaa
    59761 tttatgcaaa aatatggaaa gccattttca caaaattttt tcttcatact cgaaactaaa
    59821 cgaatttttc acaacttctc gtcaatcact aggattatta aagctttact taccttttga
    59881 ccgaaccatt tcaaatttga caaaaaacca gtataataag gaagtatcga aaaaatatcc
    59941 ataaaaggaa tgtggaaaat ggacaaccag ctgacaaaag aactaaaaga aaactataac
    60001 atcgtttttc acgatctaaa tttgcttgaa caagctttta cccattcatc ctatgtgaat
    60061 gagcatcgca atctgcaatt atcggataat gagcgacttg aatttttagg agatgccgtt
    60121 ttagaactga tggtttctca gtatttattc cgtttatatc ctcatttgcc agaaggaaaa
    60181 ttgacaaaaa cacgagctgc gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa
    60241 tgtcactttg atcaatatgt tttgttgggc aagggagaag agaattcagg aggacgtacg
    60301 cgaccagcat tgctttgcga cctattcgaa gcttttttag gcgcgttata tcttgaccag
    60361 ggattcgaca cgacccatgc attcatcgaa aaagtgattt ttcctaaagt caaagcaggt
    60421 gctttttcac atgagatgga tcataaaaca aaactacaag aagtcttgca aaaatcaggt
    60481 gatgtatcaa tcgaataccg attgatcaat gaagaaggtc cagctcatga acgagtattt
    60541 tggatcgaag tttatgtaga tgatcaattg attggcactg gacaagggaa atcgaaaaaa
    60601 ttagcagaac aagctgcggc ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa
    60661 agctttgtat ttgaaacgaa ttgaaatagc cggttttaaa tcatttgcag acaagacagt
    60721 cattgatttt gaaaatagcg tcacagcagt ggttggacca aatggcagcg gaaaaagcaa
    60781 tattacagag gctatccgct gggtattagg agaacaatct gctaaaagtc tgcgaggcgg
    60841 aaaaatgccg gatatcatct ttgccggttc cgatacccgt aagcagctga atattgcaga
    60901 agtcacagtg attttagaca atagtgatca ttatctgcca ttggagtaca atgagatcag
    60961 tgtaacccgc cgttatcgac ggactgggga aagcgagttt ttcatcaata aacaaagctg
    61021 cagattaaaa gatatccaag aattgttctt agattctgga ttaggaaaag aatcgttctc
    61081 aatcatttct caaggaaaag tagaagcgat tttcagtagt aaaccggaag accgtcgtgg
    61141 gatctttgaa gaagcagctg gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca
    61201 gaagcttttt gaaacagaag acaacctcag ccgcgtccaa gacatcatcc acgaattgga
    61261 agaacagtta actcctttgg ccgcccagag tgaggcagca aaggagtttt tacggctaaa
    61321 agaaacactg actcaaaccg atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa
    61381 agattgggac aacaaacaag cacagcttgc gaaattcaat ctagaattag gcaaactatc
    61441 tgagtcgatc caagaacaag aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc
    61501 cgatcgtctg atcgaaaaaa atcagcaagt attattagat ctttcggaaa aactgaagca
    61561 gacagaagga caaaaagatg ttttgcagga gcggacaaag catacacaaa aaagctcgca
    61621 agaatatcaa acttcattag cagaagcaca aaaaaaggtt aaacattttg aaaagcttca
    61681 agaaagcttg atgaaagcag cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa
    61741 tctcataaaa acacagcaag aattagaaaa ataccaaaaa tcaacgaaag aattattggc
    61801 agaattaaga gatcaatatg ttgatttgat gcaagaacaa gcagctgtag gaaacgaact
    61861 gaagtatcta gaacgtcaat atatacaaga aacagcaaaa agcaagcaga cgttagccaa
    61921 gcaatcggaa gtagaagcat cagtagaccg attgatctta caaaaagaag aattgacaca
    61981 aaagcaggcg cagctaaaat catctttaac tgaaacaaaa gagaagttag aaatgatcca
    62041 acaaaacggg aaaaagttcc aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt
    62101 aatgaatcaa gtccagcagc tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa
    62161 ctattttggt ttttaccaag gggttcgatt ggtcttgcag cataaacagc agctttcggg
    62221 tattgtcgga gcagtggctg aactgatcga tgttcctgca gatttcacgt tagctatcga
    62281 aacagcttta ggtggggcag cccagcatgt catcgttgaa aatgaaaaag atgctcggca
    62341 agccatcacg tatttgaaac agcaaagagg aggaagagca acattccttc cactgacgac
    62401 aatcaaacca agacaattac cggctcatat attgacacaa gcagcagctg tagaagggtt
    62461 tatagggatt gccagtgaac aagtcagtta tcccgatcaa atccaaactg tggttcaaaa
    62521 cctgctagga acgatccttt tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac
    62581 gatccgctat caatatcgag tggtttcctt agaaggtgat gtgatgaatg ccggaggatc
    62641 catgacaggt ggagcgaata aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga
    62701 attgaaacag ttgactagtg aatttgaaga agcagataaa caactccaag cacaagaaaa
    62761 aaaagttcag gagctgcaac aagaaacagc tcgtttagcc gaagaacaag aagttttgcg
    62821 tacacgaggc gaacaattac gttttgaaga acaagaagcg acaaatcagc tacaaaacat
    62881 cataaatgaa cttgaacgtt ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga
    62941 actgcagcag tttatcgaag attacgaaaa gcaaacgaac gaattgaaag acaaacaaac
    63001 agatcttgaa tcgcaacgac aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag
    63061 cgaccagatg gaagccagaa gagcacaagt acaaagtcaa aaagcacaag aacaagcaga
    63121 tcttgctgtc ttgaaagagc agtttaatca tctgcagatc cagctccgcg gtgcccgagt
    63181 ccaaaaagca gaggcaaccg aacgtcaaga agcgatagaa aagcagttgg cgacattgac
    63241 agcagacttt tctgatcatg aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt
    63301 atctgctcag cgagaaacat taaaagccga gttggcaaaa gccaaagagc agcgagatag
    63361 aaggcaaaag gagatcgata agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa
    63421 agcacgactg tcggaacaat ccaaattaga agtccaaaaa gaccgagctg aaatgatttt
    63481 agataatcat ttgacctatc tgcagtcaga atatcagctg acatttgaga aagcttccca
    63541 agactatcaa gaaacaaccg atatcgaaga cagtagaaca aaggtttcat ccttaaaaga
    63601 acagattgaa aaactgggac cagtaaactt aaattcaatc gaacaatacg aacaagtcag
    63661 tgaaagacat acattcttag caacacagcg agatgatctg ttagcagcga aaaaccaatt
    63721 gtttgaaacg atggatgaga tggatgatga agtgcggaca agattcaaag aagtatttga
    63781 agctatccgt caagaattca aaattgtctt tccaaatatg tttggcggcg gtaaagctga
    63841 attagtactg acggaaccaa ctgatttgct gaaaacaggt attgagattg aagtacaacc
    63901 tcctggcaaa aaactgcaaa gtctaagttt gctttctgga ggagaacgag cacttacagc
    63961 catcgcgttg ctcttttcca ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga
    64021 ggtagaagca gcgttagatg aagccaacgt gaaacgtttt ggtcgttact tgagcgattt
    64081 ccaagatgac acacaattta tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga
    64141 tgtactgtac ggagtgacga tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat
    64201 ggaagatatc aatgaagagg cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc
    64261 aaattagtag caatcgattt ggacggtaca ttattaaatg aacaaaagct gatttcagat
    64321 gagaataaac aagcattagc acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca
    64381 ggacgcccct tagctgcgat ggcgcattat ctgcaagaat taggactggt agatgaaggt
    64441 gactacagca tcactttcaa cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg
    64501 gaaaagaaag tgatggaagt atcagatatc caacgcttat acaaactggc tcagcaattg
    64561 gatctgccac tagatgtttt gtctgacaat gtggttttac agctgcctag tgccccaagg
    64621 aagcaatcct tgtacaatgt gttgaataag ctgctgcaat tccaaccagc ggatataagc
    64681 gatattacag atgaatttgt gcttaataag gcagtaatcg cttatgacca agaagaatta
    64741 gatcctaaaa tcaaagaaat accagcagaa ttccatgaaa tgtatgagat catcaaaaca
    64801 agaagtatgc ttttggaatt tatgccaaaa ggtgttacaa aagcttatgg tatttcttta
    64861 ttggcaaaag atcttggttt gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat
    64921 gatttaccga tgatccaata tgctggacta ggcgtagcga tggagaatgc tgtgcctttc
    64981 gtcaaagaag cagcagattt cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg
    65041 atccaaaaat ttgtattaga gtagggggag tcaaatggga ttttttgata aaatcaagcg
    65101 tgcttttact ggcgaagaga aaacagaaga agttgaagta caggagaagt acgacaaggg
    65161 tttagaaaag acgcgtaaaa catttggtga acggatgaat gagttgtttg ccaattttcg
    65221 aacagtcgat gaagactttt ttgaagaatt agaagaaaca cttatcggtg cagatgtagg
    65281 ttttgaaaca gcgatacgaa ttaccgaagc attacgtcaa gaagtgaaac tcagaaatgc
    65341 gaaaaaatcg gcagaagtcc aaaatgcgat catcgagaaa cttgtggatc tctatgaagc
    65401 ggaaggaatc aatgaagtaa atgaactaaa tatccaagaa gacggactaa cagttatttt
    65461 gtttgtcggt gttaatggtg tagggaaaac aacaagtatt ggtaaacttg cccatcagtt
    65521 caaaaacgaa ggtaaaaaag tattgatggc agcagcagat accttccgcg ctggagcaat
    65581 cgaccagtta gttgtctggg gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg
    65641 aggcgatcca gcagctgtcg tattcgatgc tttggaaaga gcaaagacag agcaggcaga
    65701 tgttttacta gttgatactg caggccgact gcaaaataaa gtgaacttga tgaacgaatt
    65761 agaaaaaata aaacgagtaa tccagcggga attaccagaa gcaccccaag aaacacttct
    65821 tgtattagat gcaaccactg gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac
    65881 aacagatgtg accggtcttg ttttgacaaa attagatggt acagcaaagg gcggtatcgt
    65941 acttgctatc cgtaatgaat tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat
    66001 cgatgacttg gagccgtttg atccaaatga ttttgtcgtt ggattgttca aaggattact
    66061 aaaagaagaa taacccatgt ctaaaaagca ggagctgtga caaaagttgc agctccttgt
    66121 ttgaatgatt gtttttaaca tagctgtgtt tttgatgcct tcatttttat acttatttgt
    66181 aagcggttct caaacaattt cgttcttttt cttagacttt ttgattataa ttagattcat
    66241 atgaattgga ggtcacttaa tgatgaattt taagcaatta ttattgcatg tcaatgcgcg
    66301 cccttttatc gaccaagctc gatttggtat agaacgggaa ggacagcggg ttgatcttgc
    66361 aggaaatcta gcaaaaaccg atcatccagc aatctttggc gatcgatcct atcaccccta
    66421 tatccaaaca gattttagcg aaacacaaac agagatgatc acccctgtta ccgattctat
    66481 tcccgaatta tttcagtatc tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa
    66541 agaagagatg atctggccat taagtatgcc acctgcctta ccggaaaaag acgaagagat
    66601 cattattgca aaattaaaaa atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga
    66661 atacgggaag cgaaaacaga tggtaagcgg tattcatttc aattttgaat ttggcgatga
    66721 gttgctaaga acattgttca gccatcagga agaatttcaa gatttttccg aattcaaaac
    66781 agaactttat ttgaaaacag cgagaaactt tatgcgctat cgctggatga tcacttattt
    66841 attcggtgct tcaccaatga gtgagaagaa ctacttttta gatgaatcac atccgcaaga
    66901 acctgttcgc agtattcgca acagtgcgct gggctatacg aatcatccaa atgtgaaagt
    66961 ttcctatgct tctatgaaac agtatttagc agatatcgag cgaatgatcg aagaagggaa
    67021 actttcggag gagaaagaat tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc
    67081 agatttagca acaacaggtg ttcgttatat tgaattgcgg aatatcgatt tgaatcctta
    67141 tgcaagattg ggtatcaatc cagagcaagt tcggttctta caattattcc tgatgtacat
    67201 gttatggaca gaagaaaaag aagactgtga ccaatgggta gcagaaggta cgactcgaaa
    67261 taacaaagta gcactagaac aaccatcaga tcaaacagaa tttcatcaag aaggtagaga
    67321 aattcttgaa ggaatgaagc aaatgctggt tgaattggat tggttggatt ctctttattt
    67381 agtagaggaa gcattgactc aaatggatca tcctgaacaa acgttagcag cgaaactcta
    67441 tcaagaagca cagctatcga gtcagcaaga agttgctgtg gcattgggac atcaatatta
    67501 taaagaaagc catgaacgtc cctatcagtt ggccggtttt cgcgaaatgg aactttctac
    67561 tcagatattt atgttcgatg ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc
    67621 ggatcaattt ttgcgtctgc aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat
    67681 gacaagtaag gacagctata tcgtcccact tatcatggaa aacaaaacag taacgaaaaa
    67741 agtcttaaaa gaagctgggt tccgggtacc aggaggcgca gaattttcat ccatggaaga
    67801 agcggtaaaa gcttatccga gatttgccga gcaggcattt gtgatcaaac ctaaatcaac
    67861 caattacggg ttaggtatca cgattttcaa ggaaggagct agtctggaag attatcaagc
    67921 aggtttagcc attgcttttc gtgaagacag ttcggtttta gtagaagaat ttatgccggg
    67981 aacggaatat cgtttctttg tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc
    68041 tgctaatgtt attggtgaca gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag
    68101 tgaccctttg cgtggaacga atcatcgtgc acctttagaa ttgatccaat taggtgaatt
    68161 agaacaattg atgttgaaag aacaagggtt gacgatcgaa tcagttcccc aagccaatca
    68221 aattgtttat ctaagggaga actcaaatat cagtacaggt ggtgattcga tcgatatgac
    68281 tgatgaattt tctgaagctt acaaaaaaat cgccgtttct gcggtagaag cactaggagc
    68341 taagattagt ggtatcgatt tgattatacc agataaagaa atcgatccta caactgataa
    68401 aaaagcatat gggatcatcg aagcaaattt caatccagcc atgcatatgc atgtttatcc
    68461 ttttgcagga aaaggcagaa gattgacgat gaatgtattg aaattgttat atcctgaagt
    68521 attttaaaag aaaaagcttt tgacagattg tcctatttga tgtcaatctg atcaaaagct
    68581 ttttttgcat gtaaaaattt atgtgttttt tcgttttaaa ataaacgatc ccagaagctt
    68641 tttttctttt ttggtttttc tttttcagct ggcgaagagt tttcttctgg gtattttttt
    68701 tcgatatcaa tcacaggttc atttactgct tcttttgctg ttaagagcaa tccgaattcc
    68761 tcaggatttg cagtgacgta atcgttgact acagtgaaac ggctattgtt tttcgttgct
    68821 agctggatat attggttttg gacagaaagg ggtagagccc cattcaagag tatggaagca
    68881 tctggatgtt ttttgagttc ttttaaaaag ttttctttgt tttttggttg gatcatttca
    68941 gcaacagtca tactaagata gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct
    69001 tcagggttca ctaaaggtgt accataaagt cccttatcca aatggctttt tacatcatct
    69061 tgtgccataa aaaataccct ccttcttctc tttgtctttc ttacgattat agcatattcc
    69121 caataatacc taaaacatat gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata
    69181 agaatgaaaa tcattctcaa ttagaaaggt ggattggatg cgctacggac gactagtagc
    69241 attattagtt attttaagta gtatttcatt gttcatcgga gtatcgaatg tacaattgtc
    69301 agaactgctc cagttagatg ggcaaagctt acaaatcttg ctagtgactc gtttgccacg
    69361 aacgatttgt ttgatccttg tgggagcgac cagtagtatt tgtgggttga tcatgcaaca
    69421 tttgacccaa aataaatttg tttcaccaac gactgcgggc acgatggata gtgcgcgttt
    69481 aggtatttta gtggctatga ttttcctacc tggtgcatcc ttgattgtcc gatccttgac
    69541 agcattttgc tttgcttttg caggaacatt gcttttttta tcccttacgc gattgttcct
    69601 ccagaaaaat caattgatgc ttccgttgat tggagtcatg ttagggaata tcattggttc
    69661 agttgctact ttttttgctt atcaattcca gttggtacaa aatatgactt catggcttca
    69721 agggaatttt gcgactgtga tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt
    69781 gttgctcttg gtttggctat ttgcttaccg atttacgatc attggtctgg gcgaatcgtt
    69841 tgcagtaaat ctgggaatag attaccagaa gatgcagttt ttaggcattg gcttagtatc
    69901 attggctagt gcagtcatgt tgatcatggt aggaagcatt ccttttatcg gagtgatcat
    69961 tccgaatctt gtctctagaa tatatggaga tcaagtgcac aaaacaattg gcgtaactgc
    70021 tttagcaggt agtttgttct tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc
    70081 ttatgaaata ccagtcagtg tgatcgttgg aatcatcggt ggggctatct tcctttatct
    70141 tctgataagg ggacgacgat atgcttaaaa aaaagccatc tatttttatt ggtatgcttg
    70201 ttcttgtttt atttgttaca ggaatgtact tgacgatcca tacatatgga aactggcatt
    70261 ttgcatgtgc attgaggagt aagaaaattt ttgcatttat ccttgtctcg ctagctgtca
    70321 gcagtgccac aatcagcttt cagacaatta cgcataacca gtttttgact ccttcagtat
    70381 tgggacttga gtcgttatat gtccttgtcc aaacggtcct tttcttcttt atcggaggag
    70441 tgacgatgct tcagcagaca ggtacagttt ttttcttatt gaacgttggg ctgatggtcg
    70501 ggctgagtct attgttattt ttctttcttt tgaaaaaaat gagagcgaat ctttttttat
    70561 tactgatgtc agggatgatt ttaggtacgc tttttggtag tgtcagtacc tttttacaag
    70621 tcatcatgga tccgaatgaa tatgatttgc ttcaaggcag attatttgca agctttggca
    70681 atatccagag tcagtatctt ttgcctgctt ctttgatcat tatcggctgc tggtacctgt
    70741 tatttcgata tcgacatgtg ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc
    70801 tcgggataga cacagatcgt tttgaatttt tgattttatg ttgtgtcagt cttttgacag
    70861 ggacatcaac ggctcttgtg ggtccagtta cttttttagg gtttattgtc gcaaatatca
    70921 gttatcgtgt cttttctact tatcgtcatg gtcagctatt tatcggaagc tttctggtag
    70981 ctatgctgtt tctgacagga ggacagctcg cagtagagca gattttcggc tggaatacaa
    71041 cagtgagtgt cgtcgttgaa tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg
    71101 aaaggaagag tatcctatga ttgatttaca aaaggtcaat aaactgtacc atggaaaatc
    71161 agtggttaaa gatgtacagt taacgattcc tgaaaagaaa ttgactgctt ttatcggtcc
    71221 aaatggtgct ggaaagtcaa cggtgttgtc gatgatcagt cgtttgatac caaaagatac
    71281 aggtgaaatc tatttagacc ataacgaagt aaaggcttgg aaatccaatg aattagccaa
    71341 aaaattatcg atcctacgac aatcgaatga aataaatgtc caaatcaccg tgaaagaatt
    71401 agtagaattt ggacgatttc cttatacgaa aggaaaattg acgacaaaag acgaggaaat
    71461 cgttcaggaa tcactggaac accttgggtt agaagaaata gcggatcaat cgattgacac
    71521 tctttcaggc ggtcaactcc aacgtgcgta tattgcaatg gtgctagcgc aagacacgga
    71581 ttatatttta ctggatgaac ctttgaacaa tctagatatg aattttgcgg ttcaaatcat
    71641 gcagatttta aaaaatttag tacaagaaat cgggaaaaca attgttattg ttcttcatga
    71701 tatcaacttt gctgcaagtt atgcagacga aattgttgca atgaaagatg gacagttatt
    71761 tacgcaagga acgacccaag aaatgataaa aaaagatgta ttggatgaat tatacgaaat
    71821 ggacatacgg atttgtgaga ttgaaggccg cagattctgt ctttatttct aaaactaaca
    71881 ttaggagaga agagatgaaa aaattagtag taggtatctt gattctagca tcatttggtt
    71941 tagcagcatg tggaaatagc aacaacacca gtcaggcaga cacaaaaaaa agcagcacac
    72001 aaactgaaac tacattgacg ataacagata gtaatggaga ccaaatcgag gtgccaaata
    72061 atccagaaaa agtcgtcgta ttcgacaatg gttctttaga tacgatggat gctcttggtg
    72121 ttggagataa ggtagttgga gcagctacaa gcagccttcc tgagtatctt tcatcctata
    72181 aaaaagtaga atcagcaggt ggaataaaag aaccagactt agaaaaaatt aatcagcttc
    72241 agccagatct aatcatcatt tctggccgac aaagagattt tcaaaaagat ctaagtcaaa
    72301 ttgctccgac cattttcctt tctttagatg cgaaaaatcc atgggaatct ttccagcaaa
    72361 atgtcactgc tttaggggag atctttggta agcaagaaga agctaaaact caattagagg
    72421 aattatctag tgcaatcgat cagacgaaaa agaaagcaga agctactgac aaaaaagcac
    72481 tagtcacact tgtgaatgaa gggcaattat cagcctacgg atctggttct agattcggtt
    72541 tcatccatga tttatttggc tttgaacaag cagatgatca aatcgaagca tccactcacg
    72601 gtcaaagtgt ttcttatgaa tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag
    72661 acagaacaaa agctattggt ggagacgaca gcaaagatga tatttctgcc aatgaactgg
    72721 tagcacaaac gaatgctggt aaaaatcagc agatcatctc ccttgaacca gatgtctggt
    72781 atttaagcgg gggcggttta gaatcaatga aactgatgat cgaagatgtg aatcaagcat
    72841 ttaaatagca taaataacag aggattaaac tctcatacta tgtaacaata atgtttctta
    72901 cattcttaaa tagcaagagc ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg
    72961 tattgatgat tttacggatg gtttttattt atttgagaaa tcttttttca acgtgaattc
    73021 attatctaca tcatacgggt tgattctaga atggggagtg gcaaaaaata gtgtgatcca
    73081 ttttttcagc atcaagatcg cctcctttga tttgaatcaa aggtacaaag cgaaacttag
    73141 ataaaactta aatttagata tttttatttt gtaccgatct cttctttaaa cgatgttcca
    73201 tctgctgctt ctcggcaata agtccactag actagaaaat atgagaagct attttctgtc
    73261 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acgatgttcc
    73321 atctgctgct tctcggcaat aagcccacta gactagaaaa tatgagaagc tattttctgt
    73381 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    73441 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    73501 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    73561 cttactccac cgttttaata attgatagaa ataactctct tctgctttga tttt
//
LOCUS       DACDID010000002        54488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDID010000002 DACDID010000000
VERSION     DACDID010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.002"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV236_000083"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV236_000083"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8770310.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV236_000084"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV236_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8770311.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV236_000085"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV236_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8770312.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV236_000086"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV236_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8770313.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV236_000087"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV236_000087"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8770314.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV236_000088"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV236_000088"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8770315.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV236_000089"
     CDS             complement(8053..8523)
                     /locus_tag="IV236_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8770316.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV236_000090"
     CDS             complement(8569..8994)
                     /locus_tag="IV236_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8770317.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV236_000091"
     CDS             complement(9061..9330)
                     /locus_tag="IV236_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8770318.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV236_000092"
     CDS             complement(9341..10936)
                     /locus_tag="IV236_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8770319.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV236_000093"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV236_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8770320.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV236_000094"
     CDS             complement(14489..15049)
                     /locus_tag="IV236_000094"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8770321.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV236_000095"
     CDS             15402..16376
                     /locus_tag="IV236_000095"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8770322.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV236_000096"
     CDS             16546..17946
                     /locus_tag="IV236_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8770323.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV236_000097"
     CDS             complement(18024..18746)
                     /locus_tag="IV236_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8770324.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV236_000098"
     CDS             complement(18825..19331)
                     /locus_tag="IV236_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8770325.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV236_000099"
     CDS             19454..21253
                     /locus_tag="IV236_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8770326.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV236_000100"
     CDS             complement(21321..22895)
                     /locus_tag="IV236_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770327.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV236_000101"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV236_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8770328.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV236_000102"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV236_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8770329.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV236_000103"
     CDS             complement(24672..25310)
                     /locus_tag="IV236_000103"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8770330.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV236_000104"
     CDS             complement(25311..25961)
                     /locus_tag="IV236_000104"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8770331.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV236_000105"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV236_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8770332.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV236_000106"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV236_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8770333.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV236_000107"
     CDS             complement(29104..29844)
                     /locus_tag="IV236_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8770334.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV236_000108"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV236_000108"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8770335.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV236_000109"
     CDS             complement(31215..32153)
                     /locus_tag="IV236_000109"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8770336.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV236_000110"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV236_000110"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8770337.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV236_000111"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV236_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8770338.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV236_000112"
     CDS             complement(35313..35618)
                     /locus_tag="IV236_000112"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8770339.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV236_000113"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV236_000113"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8770340.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV236_000114"
     CDS             complement(36724..37008)
                     /locus_tag="IV236_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770341.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV236_000115"
     CDS             complement(37026..37916)
                     /locus_tag="IV236_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8770342.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV236_000116"
     CDS             complement(37936..38154)
                     /locus_tag="IV236_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770343.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV236_000117"
     CDS             complement(38124..39824)
                     /locus_tag="IV236_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8770344.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IV236_000118"
     CDS             40091..40864
                     /locus_tag="IV236_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770345.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV236_000119"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV236_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8770346.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IV236_000120"
     CDS             complement(41688..42659)
                     /locus_tag="IV236_000120"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8770347.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV236_000121"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV236_000121"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8770348.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV236_000122"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV236_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8770349.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IV236_000123"
     CDS             complement(45614..46438)
                     /locus_tag="IV236_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8770350.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IV236_000124"
     CDS             complement(46411..47091)
                     /locus_tag="IV236_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8770351.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IV236_000125"
     CDS             complement(47114..48052)
                     /locus_tag="IV236_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8770352.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IV236_000126"
     CDS             complement(48297..49148)
                     /locus_tag="IV236_000126"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8770353.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV236_000127"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV236_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8770354.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IV236_000128"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IV236_000128"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8770355.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV236_000129"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV236_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8770356.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT        15041 a        11859 c         9789 g        17799 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattt
//
LOCUS       DACDID010000003        53262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDID010000003 DACDID010000000
VERSION     DACDID010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.003"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..809
                     /locus_tag="IV236_000130"
     CDS             <1..809
                     /locus_tag="IV236_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8770357.1"
                     /translation="SGCSKWIDRGESITAVGSSALQPLVETVAETYQTENPGKFINVQ
                     GGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIVNKDIGVKD
                     ISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGETAVQAQEQ
                     DSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWTIWAYEHMY
                     TRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE"
     gene            924..1844
                     /gene="pstC"
                     /locus_tag="IV236_000131"
     CDS             924..1844
                     /gene="pstC"
                     /locus_tag="IV236_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8770358.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1844..2728
                     /gene="pstA"
                     /locus_tag="IV236_000132"
     CDS             1844..2728
                     /gene="pstA"
                     /locus_tag="IV236_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8770359.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2743..3549
                     /gene="pstB"
                     /locus_tag="IV236_000133"
     CDS             2743..3549
                     /gene="pstB"
                     /locus_tag="IV236_000133"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8770360.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3565..4323
                     /locus_tag="IV236_000134"
     CDS             3565..4323
                     /locus_tag="IV236_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8770361.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4336..5013
                     /gene="phoU"
                     /locus_tag="IV236_000135"
     CDS             4336..5013
                     /gene="phoU"
                     /locus_tag="IV236_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8770362.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5251..6282
                     /locus_tag="IV236_000136"
     CDS             5251..6282
                     /locus_tag="IV236_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8770363.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6279..7046
                     /locus_tag="IV236_000137"
     CDS             6279..7046
                     /locus_tag="IV236_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8770364.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7146..8714
                     /gene="liaX"
                     /locus_tag="IV236_000138"
     CDS             7146..8714
                     /gene="liaX"
                     /locus_tag="IV236_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8770365.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9014..9331
                     /locus_tag="IV236_000139"
     CDS             9014..9331
                     /locus_tag="IV236_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8770366.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9331..9681
                     /locus_tag="IV236_000140"
     CDS             9331..9681
                     /locus_tag="IV236_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8770367.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9848..10786
                     /locus_tag="IV236_000141"
     CDS             9848..10786
                     /locus_tag="IV236_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8770368.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10801..11631
                     /locus_tag="IV236_000142"
     CDS             10801..11631
                     /locus_tag="IV236_000142"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8770369.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11675..12700
                     /locus_tag="IV236_000143"
     CDS             11675..12700
                     /locus_tag="IV236_000143"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8770370.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12715..13653
                     /gene="galU"
                     /locus_tag="IV236_000144"
     CDS             12715..13653
                     /gene="galU"
                     /locus_tag="IV236_000144"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8770371.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13778..14218
                     /locus_tag="IV236_000145"
     CDS             13778..14218
                     /locus_tag="IV236_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8770372.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14221..14823
                     /locus_tag="IV236_000146"
     CDS             14221..14823
                     /locus_tag="IV236_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8770373.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14882..15988)
                     /locus_tag="IV236_000147"
     CDS             complement(14882..15988)
                     /locus_tag="IV236_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8770374.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16198..17199
                     /gene="ccpA"
                     /locus_tag="IV236_000148"
     CDS             16198..17199
                     /gene="ccpA"
                     /locus_tag="IV236_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8770375.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17536..19881)
                     /locus_tag="IV236_000149"
     CDS             complement(17536..19881)
                     /locus_tag="IV236_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8770376.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20160..21062
                     /locus_tag="IV236_000150"
     CDS             20160..21062
                     /locus_tag="IV236_000150"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8770377.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21275..23050
                     /locus_tag="IV236_000151"
     CDS             21275..23050
                     /locus_tag="IV236_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8770378.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23116..24702)
                     /locus_tag="IV236_000152"
     CDS             complement(23116..24702)
                     /locus_tag="IV236_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8770379.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            24953..25177
                     /locus_tag="IV236_000153"
     CDS             24953..25177
                     /locus_tag="IV236_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8770380.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25319..27070
                     /locus_tag="IV236_000154"
     CDS             25319..27070
                     /locus_tag="IV236_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770381.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27070..28854
                     /locus_tag="IV236_000155"
     CDS             27070..28854
                     /locus_tag="IV236_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770382.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29180..30031
                     /locus_tag="IV236_000156"
     CDS             29180..30031
                     /locus_tag="IV236_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8770383.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30085..31836
                     /locus_tag="IV236_000157"
     CDS             30085..31836
                     /locus_tag="IV236_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770384.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31840..32010
                     /locus_tag="IV236_000158"
     CDS             31840..32010
                     /locus_tag="IV236_000158"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770385.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32050..32952)
                     /locus_tag="IV236_000159"
     CDS             complement(32050..32952)
                     /locus_tag="IV236_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8770386.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33183..34586
                     /locus_tag="IV236_000160"
     CDS             33183..34586
                     /locus_tag="IV236_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8770387.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34586..36019
                     /gene="ascB"
                     /locus_tag="IV236_000161"
     CDS             34586..36019
                     /gene="ascB"
                     /locus_tag="IV236_000161"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8770388.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            36164..37090
                     /gene="trxB"
                     /locus_tag="IV236_000162"
     CDS             36164..37090
                     /gene="trxB"
                     /locus_tag="IV236_000162"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8770389.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37304..39031
                     /locus_tag="IV236_000163"
     CDS             37304..39031
                     /locus_tag="IV236_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8770390.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39103..39396
                     /locus_tag="IV236_000164"
     CDS             39103..39396
                     /locus_tag="IV236_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8770391.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(39488..40348)
                     /locus_tag="IV236_000165"
     CDS             complement(39488..40348)
                     /locus_tag="IV236_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8770392.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            40440..40868
                     /locus_tag="IV236_000166"
     CDS             40440..40868
                     /locus_tag="IV236_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8770393.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            41030..41206
                     /locus_tag="IV236_000167"
     CDS             41030..41206
                     /locus_tag="IV236_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8770394.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            41233..41679
                     /locus_tag="IV236_000168"
     CDS             41233..41679
                     /locus_tag="IV236_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8770395.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(41833..43212)
                     /gene="brnQ"
                     /locus_tag="IV236_000169"
     CDS             complement(41833..43212)
                     /gene="brnQ"
                     /locus_tag="IV236_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8770396.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            43918..44889
                     /locus_tag="IV236_000170"
     CDS             43918..44889
                     /locus_tag="IV236_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8770397.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            44913..47105
                     /locus_tag="IV236_000171"
     CDS             44913..47105
                     /locus_tag="IV236_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8770398.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            47122..47598
                     /gene="ybeY"
                     /locus_tag="IV236_000172"
     CDS             47122..47598
                     /gene="ybeY"
                     /locus_tag="IV236_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8770399.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            47576..47977
                     /locus_tag="IV236_000173"
     CDS             47576..47977
                     /locus_tag="IV236_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8770400.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            47992..48891
                     /locus_tag="IV236_000174"
     CDS             47992..48891
                     /locus_tag="IV236_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8770401.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            49034..49846
                     /gene="recO"
                     /locus_tag="IV236_000175"
     CDS             49034..49846
                     /gene="recO"
                     /locus_tag="IV236_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8770402.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            50230..51147
                     /gene="glyQ"
                     /locus_tag="IV236_000176"
     CDS             50230..51147
                     /gene="glyQ"
                     /locus_tag="IV236_000176"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8770403.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            51149..53224
                     /locus_tag="IV236_000177"
     CDS             51149..53224
                     /locus_tag="IV236_000177"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8770404.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17298 a         9450 c        11357 g        15157 t
ORIGIN      
        1 tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg
       61 ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat
      121 tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag
      181 tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat
      241 tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg
      301 taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga
      361 gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa
      421 cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac
      481 aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg
      541 ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga
      601 cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa
      661 gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac
      721 aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg
      781 attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa
      841 cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa
      901 acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa
      961 aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg
     1021 tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc acctttttta
     1081 ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg
     1141 gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga
     1201 cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg
     1261 agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta
     1321 ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta
     1381 tttttggcgg gactgggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct
     1441 tgccgactgt cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg
     1501 aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag
     1561 cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag
     1621 ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacaggt cttactactc
     1681 ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag
     1741 ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta
     1801 atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc
     1861 agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc
     1921 agctttacta ctttatatct tagttcgggg aataccgcac atttcatggt ccttcttgac
     1981 cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat
     2041 ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta
     2101 tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat
     2161 cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca
     2221 attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc
     2281 tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc
     2341 aggattggcc ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc
     2401 gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc
     2461 tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc
     2521 attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat
     2581 ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc
     2641 tgccgtgttg attattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg
     2701 attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg
     2761 aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat
     2821 ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa
     2881 aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg
     2941 ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa
     3001 ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg
     3061 gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg
     3121 aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa
     3181 caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct
     3241 ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg
     3301 ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca
     3361 cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca
     3421 gctcgtatca gcgactatac tgcctttttc tatttaggaa aagtaatgga atatgacaaa
     3481 actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat
     3541 tttggttaag acggaggcct caacatgaca aaagagatta tttcatctaa agatcttcat
     3601 ctgtattatg gaaagaaaga agcactaaaa gggatcgatc taagcttcaa tcaaggggaa
     3661 atcactgcga tgatcggtcc ttctggttgc ggaaaatcca cttatctgcg ttctttgaac
     3721 cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa
     3781 gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc
     3841 cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta
     3901 aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg
     3961 tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg
     4021 cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg
     4081 gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta
     4141 gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt
     4201 atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa tgatacgaag
     4261 aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga
     4321 taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat
     4381 cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac
     4441 atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac
     4501 atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca
     4561 actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc
     4621 gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg
     4681 gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc
     4741 ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa
     4801 agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat
     4861 cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc
     4921 ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact
     4981 gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt
     5041 taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag
     5101 aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga
     5161 tgcaagcttt tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc
     5221 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
     5281 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
     5341 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
     5401 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
     5461 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
     5521 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
     5581 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
     5641 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
     5701 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
     5761 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
     5821 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
     5881 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
     5941 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
     6001 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
     6061 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
     6121 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
     6181 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
     6241 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
     6301 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
     6361 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
     6421 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
     6481 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     6541 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
     6601 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     6661 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     6721 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     6781 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     6841 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     6901 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     6961 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     7021 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     7081 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     7141 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     7201 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     7261 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     7321 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     7381 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     7441 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     7501 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     7561 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
     7621 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     7681 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     7741 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     7801 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     7861 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     7921 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     7981 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     8041 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     8101 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     8161 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     8221 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     8281 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     8341 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     8401 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     8461 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     8521 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     8581 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     8641 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     8701 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     8761 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
     8821 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
     8881 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
     8941 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
     9001 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
     9061 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
     9121 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
     9181 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
     9241 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
     9301 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
     9361 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
     9421 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
     9481 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
     9541 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
     9601 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
     9661 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
     9721 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
     9781 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
     9841 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
     9901 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
     9961 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
    10021 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
    10081 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
    10141 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
    10201 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
    10261 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
    10321 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
    10381 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
    10441 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
    10501 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
    10561 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
    10621 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
    10681 agtagccgcg atgaatttcc gcgcacgtac aatgggcttt gacgcaacga aaacatttga
    10741 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
    10801 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
    10861 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
    10921 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
    10981 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
    11041 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
    11101 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
    11161 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
    11221 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
    11281 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
    11341 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
    11401 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
    11461 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
    11521 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
    11581 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
    11641 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
    11701 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
    11761 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
    11821 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
    11881 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
    11941 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
    12001 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
    12061 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
    12121 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
    12181 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
    12241 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
    12301 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
    12361 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
    12421 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
    12481 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
    12541 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
    12601 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
    12661 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
    12721 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
    12781 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
    12841 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
    12901 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
    12961 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
    13021 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
    13081 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
    13141 ttaatcgatg attacgaacg tactcatgct tctacgatcg ccgttatgca agttcctcat
    13201 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
    13261 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
    13321 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
    13381 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
    13441 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
    13501 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
    13561 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
    13621 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
    13681 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
    13741 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
    13801 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
    13861 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
    13921 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
    13981 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
    14041 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
    14101 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
    14161 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
    14221 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
    14281 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
    14341 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
    14401 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
    14461 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
    14521 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
    14581 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
    14641 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
    14701 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
    14761 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
    14821 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
    14881 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
    14941 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
    15001 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
    15061 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
    15121 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    15181 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    15241 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    15301 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    15361 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    15421 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    15481 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    15541 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    15601 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    15661 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    15721 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    15781 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    15841 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    15901 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    15961 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    16021 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    16081 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    16141 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    16201 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    16261 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    16321 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    16381 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    16441 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    16501 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    16561 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    16621 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    16681 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    16741 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    16801 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    16861 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    16921 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    16981 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    17041 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    17101 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    17161 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    17221 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    17281 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    17341 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    17401 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    17461 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
    17521 gtgtattcta ttaaatcaat catctttctc atctgattct ttagttgctt gattcgctct
    17581 tgcataagct gaagcggtgt tccagtaatc tgtatagttt tcatcggtac cgccaacgcc
    17641 aaatttaaag gcgcttttct tcggtccatt tttcgcccat agcgattcaa ctgtttttcc
    17701 tggtgtttgg atggattttt tattgacagt aacttttcca ggtgtttgac cagtaaatcc
    17761 agatactttt ttcttcatca catcagatga taattcgaat ttctcatcag tgccaaaaat
    17821 gtcaggtttt gcttggtaag cttgattgat cagattggca agataattag ccgaacgtat
    17881 ccttgctttg ctgtccattc cagtattgtc atcgtgccct gcccagctac tgatggtaat
    17941 agacggagta gagacaatta gccatgaatc tcggtattca ttggtagaac cagtttttcc
    18001 gacccaatct gcttttccta gatttccgtt taagcttgag atggcatctt tgaatggtgt
    18061 agtgattttt gcattgatca cactacgcat catgtcattc atgatcgatg ccgttgcttc
    18121 cgaatagatc cggacagagt tgctttcgtg tttataaatt acatttcctg cgttatctgt
    18181 gatggaatcg atgatatagc cttcttcata gacgccgccg tttgctaaag cctgaaatcc
    18241 gttggtctgt tgaagcgtcg tgacatctac tgtacctaaa ggagcagatt caacgcccca
    18301 attgtcattt gccgggtagt tcatctttgc caagtagtgc tcgtaagcat attgtttcga
    18361 tccaccggaa ttcaacacat cttgatataa gtgataagcc gggatattgt tggaccattc
    18421 tagtgactca cggacggttt ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt
    18481 ctcgccagca ttttctcctt cttgccaagt tgttgcataa tctgatacac gggattctga
    18541 tccgataagc cccatgtcga ttgcaggtcc ataaacaaga actggtttga ttgatgagcc
    18601 agcttgacgt tctgtatcga aggcatggtt attttgattc tgactgtaat tccgtccgcc
    18661 aacgaatccg tagatgcggc cagttctgtt atccatcaat acatttccgg tttctacttg
    18721 actgccagca ccatcatcta acatgtatcc ataattttca acgccatttt gcaatgcagc
    18781 atagacatct ttatcgattg tcgaatggat tgtatatcct ttattttgga tcgtttgctg
    18841 tgccttttga taataagcgt cactgaccga tgaatcagac atatcagcgt tgtctttttc
    18901 agccaattgt tgtgcaatga ttcttgttgc ttcattcatc acagtataat acaagaattc
    18961 tctgtcagtc tgttctgcga tttgttgtgg tagaaagtct tttgtaaggt cataggcttt
    19021 tgcttcttcg tattgttctt tcgtaatttg gccttcacga tacatactga ataaaacaaa
    19081 atcttttctg gcaaggccgg cagaaagatc ttcttttagc gcgcctgtgt tcgtgtatgg
    19141 actatacgtg atcgggcttt ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac
    19201 atcttttgca tctacaccaa agattccttt tgcagcttcc tggactccag caatgttttg
    19261 tcccttatta ttacgaccaa atggtgagac attcaaatac gtagcaatga tctcatcttt
    19321 tgaaaagtat ttttctactt gtgcagaaag aatgatctcg tttgctttac gtttgaatgt
    19381 tgtttcatct gtaaggatct gctgtttgac taattgctgt gtcaaagttg atccaccaga
    19441 agagccaata ccagtagctt cagatactaa tgctcgtaaa acagctttag gaacgtaacc
    19501 ttggtgttta tcgaagtatt catcctctgt gctgataatt gcagttttca ataaaggtga
    19561 aatcttatca gatgaaatag tcgtgcgcat cagatctgtc tggatttttg atatctctgt
    19621 attatctgca taaacaatct ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc
    19681 ttttttagtc ggcaattgtg tgtcttcgac gagataggca aaatagccag cgcctattcc
    19741 aatacctaga gctgcgaaca tcaaaagaag aacagtaata aaaacagtca atgattgaaa
    19801 aactctaaaa ataatcttag gaacaaacca tttttctttt tttcttctat tctgtttttt
    19861 tcgagtattt ttgcgtggca aaagggtcac ctcaactaat ggttttattt cgctaattat
    19921 agcaaataat agaacaaaaa aacagacaag gctgtttttg gcaggaattt cttttttaaa
    19981 agagttaaaa cgtgagaaaa tgatctttat tataaaagag ttcttaagct tttctttaga
    20041 gaaaatttca gttactatgg agaacagggc aaagaaaaat caatcatttt ctttgtatat
    20101 catcttacca atatatagga attgttagta acaattgcct ttcaaggagg aatccatcca
    20161 tgttattagg ctcacacgtc agcatgagcg gaaagaaaat gctgttagga gcagcagaag
    20221 aagcagccag ttatggatct tcgactttta tgatctatac aggcgcccct caaaacacta
    20281 gaagaaaagc catcgaagag atgaatattg atgccggaaa agcgtacatg gcagaaaaag
    20341 gtctaacaaa tatcgtcgtt catgcccctt atatcattaa tttggggaat acgatcaaac
    20401 cagaaatgtt tccttttgcc gtccaatttc tgagagaaga gattgttcgt gcggaagctt
    20461 taggcgctac tcaaattaca cttcacccag gtgctcatgt aggggcagga acagaagccg
    20521 gaattgctag aattattgaa ggcttgaatg aagttttgac aaaagaacag acggcacaaa
    20581 tcgctttgga gacaatggca ggtaaaggta cggaaatcgg tcgaacattt gaggaattag
    20641 cgcaaatcat cgatggagta aaattgaatg aaaaattatc cgttactttt gacacctgtc
    20701 atacgaatga tgccggttat aatattaaag aagacttttc aggtgtaatg gaagagtttg
    20761 ataagatcat cgggttagat cggcttaaag tactccatat caatgattct aaaaatccgc
    20821 aaggttcgca taaagaccgg cacgccaata ttggttttgg aacaatcgga ttcgacacgc
    20881 tgaataagat cgtccatgat ccaatgtttg acaatatttc gaaaatcttg gaaactccct
    20941 atgtaggcaa agataaaaag aaagcttacg caccatatgg aaaagaaatc gccatgttca
    21001 aagcgcaaac attcgatcca gaagtgtttc ctgagttatt agcagaagaa gtgatactct
    21061 aagattttct atctgacagc agcaaaaaag gaccaggcac attgccttgt ccttttttgt
    21121 ttaagaaaca aaggagtact ttttgcagtc tttttcatca tgcatcttct cccccctctt
    21181 tttaaattta gacaaacaag gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa
    21241 gcaagtggca aaacgtgatt ggaaggacga aaagatgctg aacactttaa aacccttgat
    21301 caaccaacta aaaaagaaac atacatactt tattcttggc atagccctat tatttacaat
    21361 tattcgtttg tatttatata gtaaagcaat ctatggaaca gacatgtcgg caacctatga
    21421 tgaccagctg ctgattcgct atgctcaaaa tattttggac ggtaaatggc taggggagta
    21481 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
    21541 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
    21601 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
    21661 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
    21721 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
    21781 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
    21841 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
    21901 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
    21961 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
    22021 tcctatagtt ggcttttcca tcgtctatca atggatcgaa tataaaaatg agacaacata
    22081 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
    22141 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
    22201 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
    22261 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
    22321 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
    22381 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
    22441 caagggaaca atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
    22501 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
    22561 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
    22621 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
    22681 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
    22741 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
    22801 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
    22861 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
    22921 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
    22981 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
    23041 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
    23101 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
    23161 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
    23221 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
    23281 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
    23341 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
    23401 gctgttggtc atcatcagta aaggaatcat cgaaaacggc aacgcaaaag ccaagaacac
    23461 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
    23521 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
    23581 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
    23641 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
    23701 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
    23761 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
    23821 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
    23881 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
    23941 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
    24001 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
    24061 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
    24121 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
    24181 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
    24241 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
    24301 aatatctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ctaatgtctt
    24361 acttgtgcgt gctcggattg cctgtgccaa atccatttgg ctcacgattc ctaattttgc
    24421 tgccatatac tgtaaaagca tagcaatcag actagacatc aaaataactg acatcaataa
    24481 atattggaaa ttttgtcctc cggtgatcga agtagaccag ttacccgggt ccatataccc
    24541 aactgctact aacgcaccag gtccagagta agcaagaagt gttttaaaaa aacttttccc
    24601 ctttggtaca tcaactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
    24661 aagtcgatgc ttatgaaatt gtttattgga ttgattattc aagaaaaaac cacctttcct
    24721 atttatctac gattacatta tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta
    24781 cgcctaattt tttttcatgg cgcgtcttgc tagatgacga aagaaatttt gttctcctct
    24841 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
    24901 ttagtaaaat aagtcattga gagcaattcg gaaatagaaa ggagagattt atatgttatc
    24961 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
    25021 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
    25081 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
    25141 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaac gactttttac
    25201 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agattattca
    25261 gcaaaaaaga caagcacaga caatcctagg ctcttgataa agacaaagga ggagtaaaat
    25321 gtcgattttt agaaaattag gctggttttt tcgtcaggaa aagaaacatt atttaattgg
    25381 ggttttctcg ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
    25441 catcattgat gagatcgccg ataaaaattt atcaataaag atcattttcg ggtggatctt
    25501 cctattgatt atcgctgctt ttttacaata tctttttcgt tatatctggc ggacgaatat
    25561 ttggggaagt gcagctcgat tggagaaaga acttcggcag cagttattca aacattttac
    25621 agaaatggac aatctctttt accaaaaata tcggactggg gatttgatgg ctcacgcaac
    25681 gaatgatttg aatgcgatcc agaatgtagc aggagctggt atcttgacct ttgcagattc
    25741 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
    25801 actgattgcg ctgattcctc tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa
    25861 actccatgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
    25921 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctagc
    25981 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
    26041 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
    26101 tttaggtgga aagtatgtct tggaaggcac gatctcgatc gggcagatgg tctcatttat
    26161 tagttatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
    26221 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
    26281 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
    26341 ctcttttaat tatccgaaag aagaatcacc agcattagaa caaatcaagt ttgcgatcca
    26401 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa
    26461 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
    26521 caaagaatat tcattgaatg ctttattagg atcaatcggc tatgtgcctc aggatcactt
    26581 cttgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
    26641 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattaccaaa
    26701 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacaaag
    26761 gatttcgatt gctcgagcat tgatcaaaaa tcctgaatta ttaatattag atgatgcact
    26821 ctctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    26881 agagaaaaca actatcattg cagctcaccg tttaagtagc gtgatgcatg caaaagaaat
    26941 cattgttgtc gatcaaggga aaatcattga acgaggaacc catcatgagc tgcttgcact
    27001 aggcggctgg tatgaacaaa tgtgggagaa acagcaatta gaagcgaaga tagaaggagg
    27061 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    27121 cagaaaaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    27181 gttgctatct tatttgcgtt tgctttgtct gtgatcaatg tcctattgcc aaggatcatc
    27241 caaactttta tggatgatca tctggctaaa cagtcggcta cgacacaagt tattttgttt
    27301 tttgctggta tgtacttatt tggagtcata gtaaaaagta tcatttggtt tttccaatgg
    27361 tatttgtatt ccatggcctc attgaagacc tatcagtata ttagggtaaa attatttgaa
    27421 aagctgcata cactaggtat gcgttatttt gatcagacac cggcaggatc aaccgtttct
    27481 cgagtgacga atgacacaga aacgctattt gaattttggt acgtcttttt gatggtattg
    27541 actgggatct ttgcagtcgt ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt
    27601 gccttgtgga ctttggtgtt tttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    27661 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    27721 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    27781 aaagaatttg aacaagtgaa caatgagtat ttagacacgc gttatgcgat gatcaaaacg
    27841 aattcgattc tgttaagtcc gatcatcaat tttctatatg cattggcgat tgcgttggtg
    27901 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    27961 tttacgacct atgtccaggg attttttaat ccaatgtctc aaatgatgga ctttttgagc
    28021 atcttcacag atgggatggt tgctggtagt cgtatcttga agattatgga tacggaagaa
    28081 tatgcaccac aacaaaaccc tggagcagat caaatgatta cttcaggtaa gattgagttt
    28141 cgcaatgtaa gcttttctta tgatggagag cacaatgtat taaacaatat ctcttttgtt
    28201 gctaatccgg gagagaccgt tgcattagtg ggacacaccg gaagcggaaa aagctcgatc
    28261 atcaacgtat tgatgcggtt ttacgaattt tatgaaggtc agatattgat tgatgatcat
    28321 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tggggctggt cttgcaagat
    28381 gctttcttgt tttatgggga tatcgcagga aatattcgga tgatgaatcc agctatcaca
    28441 gacgaacaga tacgccatgc ggctgagttt gttcaagcaa acaaattcat cgatgaatta
    28501 ccaaaaggat atcactcaaa agtgatcgaa catggtgcca gctattctag tggacaacga
    28561 cagctgatca cttttgcgcg aacaatcgtg actgatccta aaattctagt attggatgaa
    28621 gcaacagcaa atatcgatac tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg
    28681 cgtcaaggac ggacaacgat tgcgattgcc catcggctgt caacgattcg agatgcagaa
    28741 ttgattttag ttttagacaa aggtcatata gtagaacgcg ggaatcatga aacacttcta
    28801 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtat ataaaagatg
    28861 ttgtgacagt agaattttga cctaagcact agagtgaaaa taaaacatag cttctcatat
    28921 ttttcatctg tgagagttag tgtcgtagtc accagattta aaaaaagatt cgacagctag
    28981 tcagaagtgc ctaatagcca ctaagaaagc gttgacaaaa gcatttgctt tcgataatat
    29041 aaagaaaacg atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc
    29101 ccatggtttc atggacggct ttttgggttt ttctttttgt tttggataca gccctataca
    29161 tcatggaaag gagcaaaaca tgctgattga gaaagtactg aataacaacg tcgtaataac
    29221 cagggatgaa agaaatcagg aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa
    29281 agtgggtgac ccgatttttg actcatgcat cgacaaaaaa tttcagttga caaatgacgg
    29341 gctttcacaa cagttccaag aattggcaca agcgattcct cttgagtact tagaaatttc
    29401 ttgtgaaatc attgaatata caaaagaggc tctacaagtc cagttgaatg attctgtgta
    29461 tattgcattg acggatcatt tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt
    29521 accaaatgta ttgctgtatg atatcaaaca actatttcct aaagagtatg ccatcgggaa
    29581 aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag atgaagccgg
    29641 atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccagcaag acaataccta
    29701 cctgtttact ataacaatac aggatattct aaatatcgtc cgctattact ataagattac
    29761 atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt tttttctgac
    29821 acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat tgtttcaggt
    29881 tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag actttttagc
    29941 cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga tccatactgc
    30001 gcgtttagtg catgagaatc gttgtaaata aaaaaaggag gataaattaa caagaacgta
    30061 tgaaataagg gtagtgacat tcagttggct aaaccttcca ctaataaatt ggagggaata
    30121 gaaatgggaa aataccaaga attagcgaga aaaatcgtag aaaatgtcgg cgggaaggaa
    30181 aatatcaatg gtttgacaca ttgtatcaca aggcttcgtt tcaaattaaa aaacgaagaa
    30241 aaagcaaatg atgagatctt gaaaaatatg gacggtattg taacggtaat gcgagcggga
    30301 gggcagtatc aagttgtcat tgggaatcat gtgccagtcg tgtttgaaga ggttataaaa
    30361 gcaggaaacc ttacttttga tgaggctgtt tcaacagaaa aaatgagacc gtttgatatg
    30421 cttattgatg gaatatcggg ctgtttccag ccattccttg ctatcctagc tgctggcggg
    30481 atgattcgtg gactgacagc attccttgtc ttcttaggcg cgtttgaccg cggttctggc
    30541 acatttgtta tgtttgataa tatcggtgat tctgtattcc aatttatgcc tgttattatc
    30601 ggtttaacag ctgcccgtaa atttaaagta aatgaattcg tagggatgtt gcttggcgca
    30661 gcattaatga atcctagctt gtcgctggaa gctttgagtg gtgcagcaga agcaccacta
    30721 acgaccatat tttctggcac tatttttgaa gcacctattt atcaaacagt attcggcatt
    30781 ccctggattg cgcgtaacta cgcttcttct gtcattccca ttatctttat tgtgttgtta
    30841 gctagccaaa ttcagaaacc aattaaaaag ttagtacctg agatgatcgc gaatttcttc
    30901 gttccattct tcaccgttct tattacaatg cctttaggtt ttttactcgt tggaccagta
    30961 ttcacattcg caactgatat tttaatggct ggatttgaaa cacttttagc actttcgcca
    31021 gttatttatg gagcaatcgt aggcttcttc tggcaaattc tggtgatgtt tggtctccat
    31081 tgggcaatcg taccaatggg attgatgcaa ttttctgtga atggttggca aaatattatg
    31141 acacctgttg cagtagtgtc ttttggacaa actgcagcac ttaccgcact ctactttaag
    31201 cttagaaatc caaaagacaa agcaattgca ataccagcca ttgtttctgg tattgtgggg
    31261 attacagagc cagctattta tgggtttaca ttaccacgta aaaaaatatt tatttttacc
    31321 tgtgtcggtg gtgcgttagg gggcgcgtat agtgggttaa tgaatttaac ttcttggaat
    31381 caaggtggac taggcatctt tacaattccc aactatatta gaccagatgg tgatttaaca
    31441 gatgtgatta atgtattgat tgggatcgcc attgcgatgg tggtatcctt tacgctgact
    31501 tttttctttt ggaaggatga agcaggtgaa acagatgata tacagaaaaa atcaggtaaa
    31561 gaaattgtaa aaacaccgat acaaggacaa atcgctccac taaatgcagc aaaggatgca
    31621 gcttttgcac aaggaacact aggtcgagga atcttgatct accctgaaaa aggagaagta
    31681 cgtgcccctt ttgacggaac agtcatgacc ctgttcccga cgaaacaggc aattggaatg
    31741 gtttctgaaa cagggctgga attgctgatt catgtcggat tggatactgt gcagttggaa
    31801 gggaaatatt ttgaaagctt agtgcagcaa ggataaagag tgaaacaagg cgatctgttg
    31861 ctacgcttcg acaaaaagaa aatcgaaaag gaaggataca gtttagaaac acctgtgatc
    31921 gtcaccaatt acacggatta tttggatatt ttggaaaacc aaggggagac ggtcgggcca
    31981 gacgatgcac ttatcaccgc actgacctga acggttaggt gaagaatgga ctaggcttaa
    32041 ctattctttt tattccactt ctttattttc aaaattgaaa ggctctttca tgatggctga
    32101 gctgataggg cgtttatcga atttttgacc gacagaaatt agatggacta aattctgttg
    32161 ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa ttgatgtatt
    32221 gatcgtaaaa ttaccaattt ttgaatacag tcgctcgcgt gtcgtaagtc gcaaagcggc
    32281 tgtactcatt gcagatagcg ttgaatcacc gatactggtc aaagctaaaa ttggaacatg
    32341 attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg tataagaaat
    32401 cagaatcccg cacatatcac tcgtgcaatt gtacgcttca tagaatgatt caccaaaagt
    32461 ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat cttgagaaat
    32521 caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca ttagttgttt
    32581 ggctttttgc agttggtcat gatgaatgag agaaagtgta tcatgaatcg tcatctgttc
    32641 taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa aaggaagatt
    32701 cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa aagcattacg
    32761 aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg tagaggggtg
    32821 gacataacat cgctcagcaa tcgcttggat ggtcatttcg ccaagtgctg gagaatcgct
    32881 taagagaaac tggatcactt cattttctga aggagaaaag gagatttctt tgattttttc
    32941 acgtaataac atgtagatac cttcttctta tttttttacg taaattataa cttttttgaa
    33001 aatgatttgt aaacggattt atactatata acttaaaata gacatttttt gacaagatat
    33061 ttacaaaaat ccactagaaa acagtaaatt ttgtaaatgt tactggaaag aaacagattt
    33121 ctattgtttg ttttgtttac aaaaaagata atgaaagtgt aaaaaagtaa aggagtgaga
    33181 acatggcaaa aggaatcaaa atcgctacga ttggtggagg cagcagttac acccccgagt
    33241 tgatggaagg atttatcaaa agatatgagg aattaccgat acgagaaatc tggctcgtag
    33301 atgtagaaga aggaaaagaa aagcttgaga tcgttagtca gatgtctcag cgtatgtggg
    33361 atgcttctcc gtatgaagtc aagatctatg caactctgga tcgggaagaa gcattgaaag
    33421 atgcggattt tgttacgact cagtttcgtg tcggtcaatt agatgcacga atcaaagacg
    33481 agcggatccc tagttattat gggatgattg ggcaagaaac aaatggtgct ggcgggatat
    33541 tcaaagcttt tcgtaccgtt ccagtcgttt tggcaatcgt tgaggacatg aagcgtttat
    33601 gtccaaatgc ttggctgatc aatttcgcga acccaagtgg gatggtgaca gaagcagtta
    33661 tcagatatgg caaatgggat aaagtgattg gattatgcaa tgtgccagtg atggcgatga
    33721 tgacagaacc agcaatgctt ggcaagaaaa aagaagagct gatctacaag tttgccggtc
    33781 taaatcattt ccattggcac aaagtagcag ataataaagg aaatgaccgc acggcagaat
    33841 tgattgatcg tttatacgat gaagagaatg gattaccgaa aaatatcttt gatatcccgt
    33901 ttttcaaaga acaattaaaa caaatgaaca tgatcccttg tggctaccac cgctattact
    33961 atcgtgaaga agaaatgctg gaacatgctt tagaagaata tcaaacgatt ggtacaagag
    34021 cgcagcaagt gaaagaaaca gaagcagagc tatttgaatt atacaaagat ccagaattag
    34081 accataaacc tgaacaattg caaaaacgag gtggcgctta ttattcagac gctgcctgtg
    34141 aaacgattgc ttcaatctat ggaaataaag aaacacagat cgtggtatct acaaagaatg
    34201 atggagctgt tcctgacctg ccggcagatt gtgtagtaga agtcactgcc tatgttggcg
    34261 cacaaggagc gagaaatgta gcgtttggtt ctcttcctgc tgctgaaaga ggctggttgc
    34321 aagtaatgaa gaatatggaa ttgctgacga tcgaagcagc tgtgactggt gattatggga
    34381 cagctttaca ggcgtttacg atcaatccac ttatacgatc aggaaagaca gcaaaacgga
    34441 tcatggatga gttgtttatt gcacaccagc agtatttacc gcaattcaag gagacaatca
    34501 gccgattaaa agaagaagga atcgaagttc aagatgaact ggcaagagaa ttatcaggta
    34561 atacacatat aatggaggaa gtataatggg attcagaaaa gattttttat ggggcggtgc
    34621 tacagcagcc aatcaatgtg agggcggtta tgatgaaggt ggacgcggac tagcaaacgt
    34681 agatgttgtg ccaatcggaa aagaccgttt tcctgttatc accggaaaga aaaaaatgtt
    34741 tgaatttgat gaggatcatt tttatccagc aaaaattgca atcgatatgt atcatcgtta
    34801 taaagaagat attgctttgt ttgcagaaat ggggttcaag acttatcgaa tgtccatcgc
    34861 ctggagtcgt atttttccaa tgggggatga gaaagagccg aatgaagctg gcttgaaatt
    34921 ttatgaggat gcctttaaag aatgccaaaa gcacggaatt gagccacttg tgacgattac
    34981 gcattttgat tgtccaatgc atttgatcaa agaatatggt gcgtggcgtt ccagagaact
    35041 cgttggtttt tatgaaaatc tgtgtcgagt catcttcaat cgctataaag gacttgtcaa
    35101 atattggctg acattcaatg agatcaatat gatccttcat gcaccattca tgggcgcagg
    35161 gctgtatttt gaaaaaggag aaaatgaaga acaaatcaaa tatcaagcgg cacatcatga
    35221 gttggttgca agtgccatcg caacgaagat tgctcatgaa gtagatccgg agaacaaagt
    35281 aggctgtatg ctggcagcag gagcatatta tccatacact tgcaatcctg aagatgtttg
    35341 ggcttctaga aaatcagatc gagaaaatta tttcttcatt gatgtccagt cacgaggaga
    35401 atatcccgct tatgcattga aagaaatgga gcgaaatggc attcagatcg aaatggaacc
    35461 aggggatgcc gcattgctga aagaacatac tgttgatttt atctcttttt cttattatgc
    35521 ttcccgagtt tcgactaccg acaacgaact cttggaacag actgcaggaa atatctttgc
    35581 ttctgtcaaa aatccatatc tgcaagcaag tgaatggggg tggcaaattg atccattagg
    35641 cttgagaata acgatgaatg atatttatga tcgttatcaa aaaccattgt ttattgtaga
    35701 aaacggctta ggtgcagtcg atacgccaga tgaaaatggt tatgtggaag atgattaccg
    35761 aatcgattac ctggcagcgc atatccaggc gatgaaggat gcagtagaac tagacggagt
    35821 cgatctatta ggctatacga cttggggatg tattgattta gtatcagctg gtaccggtga
    35881 gatgaagaaa aggtacggat tcatttatgt cgatcgtgat aaccaaggaa atggcacatt
    35941 agcccgttca aagaaaaaat cattcaactg gtataaaaaa gtaattgatt caaatggaga
    36001 aaatctttcg aacgattgat caatgattta taaaagtcta ttgtttttca gcggattttt
    36061 cgagtataat aaagctattc gagaaacgca agcagaacaa tagacttttt ttcgcattgg
    36121 ctgtaaagta tgcttacttg attaagtaaa ggagaatcta catatgtatg atgtaatcgt
    36181 tattggcgca ggtcctgctg ggatgactgc tgctttatat gcttcacgtt caaacttatc
    36241 cgtattgatg atcgaacaag gtgcccctgg cggccaaatg aacaatacgg cagaaatcga
    36301 aaattatcct ggattcgatt cgatcatggg ccccgatcta gcttataaga tgtatgaaag
    36361 tgtctcaaga tttgggacag aaaatgctta tggaatcgtt caagatatca aggatcacgg
    36421 cagccataaa gaagtcatct gtgaagataa aagttacgaa ggaaaaacag tcattatcgc
    36481 aacaggatgt atccaccgta aattaggcgt acctggcgag gaagaatttg ctggacgagg
    36541 cgtttcttac tgtgcagtct gtgatggtgc gtttttccgt aacaaacgtc tagtagtcat
    36601 cgggggtggg gactcagcag tagaagaagc catctatttg acacaatttg cttctgaagt
    36661 agtgatcgtc caccgtagag atgaattacg tgcacaaaag atcatccaag accgtgcatt
    36721 tgcaaatgaa aaaatttcct ttatgtggga tacagtcgtt gacgaaatcg ttggaaacga
    36781 aatggtagtg acaggtgtga aagcacgtaa tgtcaaaacc ggcaatgaat ttgatttagc
    36841 agcagacgga gtattcatct atgttggact ggatccatta actgcacctt ttagagaagc
    36901 aggtttgacg aacgaagctg gctggattcc aacagacaac gaaatgaaaa cgaatatgcc
    36961 aggcgttttt gccatcggag atgttcgtga aaaagatttg cgacaaatca ctacagctgt
    37021 tggtgacggc ggtattgctg gacaacaagt gtataaatac ttggagtctt tagaagaaac
    37081 aacagcataa gtttaaaaag agtagaagaa cgggttcaat tgttctttta ctcttttttt
    37141 tcgcgtgaaa tttactgaat ctgatttatg cgaacacgtc caacaccttt tgataaaaga
    37201 atcctttctt gatttaagcg ttttttggtc ctcatacttt catatctaat aagtgaatgc
    37261 tatacttatt aagaaccata tttcagaaag aggtgttatt ttcatgtctt gggaacaagt
    37321 gtatgaacaa tgggtacatg aagaaaattt agaagaaaac ttaaaaaaac aattaaatga
    37381 tctgggtgaa gatcctgaaa aacttgaaga tgctttttat gcaccgttgg aattcggtac
    37441 tgcaggtatg cggggaatct taggacctgg gatcaatcga atgaacatct atacggtaag
    37501 acaagcaaca gaaggactag cacgtttcat ggatacacaa ggtgaagaaa caaaacgccg
    37561 aggtgtcgct atcgcttatg actcacgcca tcagtcacca gaatttgcaa tggaagcagc
    37621 caaaacacta gcacatcaca atatcccgtc ttatgtattc gaaagtctgc gtccaacacc
    37681 agagctttct tttgcagttc gtcatttgaa gacgtttgcg ggaatcatgg taaccgcatc
    37741 tcacaaccca gcggcttaca atggatataa agtctacgga gaagatggcg gacaaatgcc
    37801 tcctgcagac gcagatgcat tgacgaaata tgtccgtgaa gtcagcaatc cgctaaaagt
    37861 cgaagtatta tctgacgaag aagctaaaca tagcgggttg atcacgatca tcggagaaga
    37921 agtggacgct gcttatctag aagaaatcaa agcagtgacg attgatcgtg aattggttga
    37981 aacaatgggg aaagatttga aacttgtgta cacgccactt catggaacag ggaaaatgtt
    38041 gggcgaacgt gcgttgaaac aagctggatt cgaacaattc gtactagttc ccgaacaagc
    38101 tgtggcagat ccagatttca ctacggtcaa atcgccaaat ccagaagaac attcagcttt
    38161 tgaatatgct atacgacttg gagaaaaaga aggagctgac ttgctgattg caacagatcc
    38221 tgatgcagac cgtttaggcg cagcggttcg tttgccagat ggttcttatg aagtattgac
    38281 tgggaaccaa atcggagcat tgtttgctca atatattttg gaagcacatc aatcaagagg
    38341 cactttacca gaaaacgcag cggtattgaa atcaatcgtt tccagcgaat tgcctacagc
    38401 aattgcggaa cactacggtg ctaccatgtt caatgtgttg acagggttta aattcattgc
    38461 tgaaaaaatc caacaatttg aagaagacca ttcacatact tttatgtttg gattcgaaga
    38521 aagttatggt tatttagtta aaccttttgt tcgcgataaa gacgcgatcc aagcattgct
    38581 tttattagca gaagtagcag cttactataa aaaacaaggc aaaacgttgt atgacggctt
    38641 gcaaacgatt tttgaaacct atggctattt cgcagaaaaa acgatttctg tgacaatgag
    38701 cggacaagaa ggttctgcga aaatctctgc tttgatggaa aaattccgta aagaagcacc
    38761 gacagaattt gccggagcaa aagttattca aacagaagac tttaaagaat tgacgagaac
    38821 agaccgtgaa ggaaacgtag aaaaactaac aacaccgcca tccgacgtat tgaagtatac
    38881 attggaagaa aatggctgga tcgctatccg cccttcagga actgagccga aaatcaagtt
    38941 ttatattggt gtaaaaggaa gctctacaga agacgcagcc gaaaaagtag cctcttatga
    39001 agcgattatc aaaaagatga cagaagaata aaaaacttga gactgatgga aaaagtggta
    39061 aactttagaa gtcagtcttt tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa
    39121 tcaaaagtag agaaagtcag tcaattattc aaaatgttga gtgatcccac tcgtcttaaa
    39181 attctgctat atctgaaaga cggggaacaa aatgtgacag caatcaccca agcagtggaa
    39241 atggagcagt ctgctgtatc gcatcaacta cgtttattaa gagaaaatca tgtcgtcaag
    39301 tctcatcgcg agggaaaagc gattttgtat agtcttgatg attctcatgt actggatatt
    39361 ttgaaccaga cactcaaaca tgtggaacat caataacgat aggtgaaaat agataaacac
    39421 tggagtgtga caacgaagaa atttcgttgt cacactccag tgttttttaa gaaaaagaca
    39481 aaaatgatta atcgcttttt tctgttgttt cagttccata ataactatga tcttctaggt
    39541 ctaaagcatt caaaataagc gaagccgttt cttcaataga taaagatgcc acattgatca
    39601 ctacacatcc tagtttttca taaagatcat tcgcaaaatc gagttcagct ctaatttttt
    39661 cgatatcgga ataagcagta tcagggttta atccgtaaga gcgcatccgt tctttacgga
    39721 tattatttaa aatatctggg ttattcgtta gtcccacgat ttttttagga tcgatttccc
    39781 acagctgttt gggaatatgt gcttggggaa tcaaaggtaa attagcaact tttaaatttt
    39841 tatttgctaa aaacaagctc aaaggcgttt tggaagttcg ggatacgcct agcaaaacga
    39901 catctgcttc taaaaaacca cgaggatctt tcccatcatc gtattttaca gcaaattcca
    39961 ttgctttgat ccgtttgaaa tagttctcat tcaataaatg gagggcacca ggttgccgaa
    40021 ttggcttgat accagttaaa cgttcaattt ctgatatcgg tggcgtcatc acatcgaaac
    40081 taaataattg atgttcttca caaaaagaac gtgttatctc gatcagttct tgattgatta
    40141 atgtatgcag gacaagtgct ttttcttcta atgcatcttg caatgcctgc cttaatgttt
    40201 tttcatctgt cacgaacgtt ctgcggaata ggttgaactc aacggtagga tactgagcca
    40261 tagtagcttg cgctagtttt gtcgcagttt ctccagcaga gtcggagatg acaaaaaaag
    40321 taacgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt tttttatgtt
    40381 tattatacca tactctagaa tgcgtacttc gtaatcattt tgatttggag gaataagtca
    40441 tgaaaaaaca gacattgatc gaacaatcat tagaaacatt gaaaaataac ggttttaaat
    40501 atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgtaaccgc tacgtatctg
    40561 ctaaagaagt ttttgactat atgaatcagg aattcaaagg ggtaagttat gatacgattt
    40621 accggaatct ccatgatttt gcagagatcg atttactgga agaaaccgat ttaaatggtg
    40681 aaatgaagtt tcgttttcat tgctgtcagg gggaaatcgg ccatcaccat cattttatct
    40741 gtacagtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac
    40801 agctaggtgg atgcacgatc gaagggcata gattcgagat tttcggaaga tgtgaaaatt
    40861 gtcaataagg atttaaataa aataacattt gataatttaa aaaaaaagca agatttcctt
    40921 gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt tgagatatat
    40981 ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca tgtcaaaaac
    41041 agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc
    41101 aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag
    41161 acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt
    41221 ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaagc agcgatgaaa
    41281 gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat acaaaacgag
    41341 cagattaaag aaggtcacga cttgacagaa gaagaagaac taacggtgtt gtctcgcgaa
    41401 atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga cgatttagct
    41461 gaaaaagtaa aaagtgaaat tgtaatagtc gaaaaatata tgccggaaca actttccgac
    41521 gaagaaattc gacaacttgt tcaagaagca atcactcaaa ctggtgcttc atcaatgaaa
    41581 gaatttggca aagttatggg tgcaattatg cctaaagtca aaggaaaagc agatggaaac
    41641 caagtgaatg cgattgtgaa agaactatta caatcttaat tttatcattt aaactgcgga
    41701 cagtgattgt ccgcagtttt ttgtgttcgt aaaaaaagaa ctaaaaaaca gctagaagaa
    41761 agaataagat tctttcttct agctgttttt ttcctgttta atatctctta tgttaaattg
    41821 tgtttgattt ggttattctt ctggtagttc atttgcttct tgtttatacc ctgataggcg
    41881 ataggcgtaa taaaagagta agctgccagc gtaaccaagt aacgttggga ccatccagcc
    41941 aagtcctaat gcagcaaaag gaacgtgatg atgataaaat cctagtaagc ttgctacgac
    42001 agtttgctga ctcactaatg caggcgaatt tgccaacata tccaatacgg ctggaacggc
    42061 agaaaagaac atcgttactt gatagacaaa tggactcttt tggaagaatt ttgctgttaa
    42121 ggaaagcaag attaacgcaa gtgacaatgg atacaaaagc atcaatactg gtaaagacca
    42181 ttggatgata ttatccaatc cggcattcgc aacgacaaaa gagacaaaag tcgttaaccg
    42241 cagccatgtc atataactga ctttagggaa cagtttatgg aaatcttgtg caaaagaaac
    42301 aacgagtccc atagcggtag tgaagacacc taatgtgacc attaccccta agaagctagc
    42361 accgaagtta cctaaataaa aattagtgat ctgagaaagt gcagttccgc cattttctga
    42421 taaaggcatc ttattcaaac tcatcgcacc gagaaggacc agaccaaaat aaatcatgac
    42481 ttctaacgca acgctcaggc taccagcttt tgctgttaat tctgtgactt ttttatcttt
    42541 aaaaccaaga tcacgaactg catggacaaa agtgacactt aaagcaagta aagctactgc
    42601 atcaaccgtg ttgtatcctt ctaaaacgcc atttgtcaaa gcgttcgtct gataagaatc
    42661 tgcagcagat tggtttagat tccccatggg ttggaataaa gaaacgacaa aaatgatagc
    42721 taataatgca agaaacaagg agtttaaata ctttccgaca tatttgacca aacgtgttgg
    42781 atgtacagta aggaaataag caagtaaaaa gaaaagagca gagaataaaa acatacctaa
    42841 tgaatcatat ttttcaggca aaaagggttt agcagccatt tcaaaagcag ttgctgctgt
    42901 ccgcggtgta ccaaagaatg gtccgatcgt taaatgaaca aggatcaaga acaaggcagc
    42961 ataccatttt ccgacaggtt tagctaagtc atacagacca tcactttttg tcactactac
    43021 agctaagata gctaataatg ggaaaagagc gcctgagatg gcaaatccta caccagcgct
    43081 gatccagttt gatccagcca tttgacctaa gtggacagga aagatcagat tacctgaccc
    43141 gaagaacatc ccgaagataa gtgaactgac catcaataat tgcttccatg aataagtttt
    43201 tgtttcttcc ataaattttt cctccaataa ttatcatttt gagtaacaaa ataaacgttg
    43261 cagaaactag aaatgccact aatatcagat tccatgaaaa ccaccacctt aaaatataaa
    43321 aaaaagccct tttccacttg ggaaaagggc tctagattct agaacggtac cacctttttt
    43381 cgcttaaaaa agcttcttca ttcacggacg cttttcgata aagttatccg ctagcctgat
    43441 aatggaggca gccacaatga tctacgtttt cgacattgac tcggaaatga tgttcgtcta
    43501 ttcgatttat tgtcatttct cacttacatg actcactggg aataaatcgc tgaatagcct
    43561 actgttttcc tcgacgcttt gctgtatatt tatttgttga cgttataata cacctgtttt
    43621 ttttgaaatg caagaatatt tcaaatattt tgttttcttt ttttgaaaca agaaaaatac
    43681 ccagagaata cttgctaagt tagacggaat acttgtcctc tatcttatgc atagcttaca
    43741 tgatcagctt tttttattca aacgagacct ttagagaatg gaagcagcta ttttttaacg
    43801 tttcttggta cttatggtta agcaactctt ttagaacctt ttcttatttg ctatatagtt
    43861 tcttctgagt atggtaaaat aggaaagaat gaatcgaaaa aaaggggatg tctatttttg
    43921 acggaagagt ctagttcttt agagattaga ttgacggctg ctgatgatgt cagcatgctt
    43981 ttaggtgcac acgacaagca tataaaactg attgaagaaa caacagaaac aaccatccat
    44041 acacgtggag aaatcattca gatcattggc gaacaatccc agatttcctt ggctgcctca
    44101 gtcattcgga cattgcagga attgatcaaa cgaggaatcc atgtaagtac accagatgtc
    44161 gtgacggcac tgaaaatggg gcaaaaaggc acattagatt attttgttga aatgtatgag
    44221 gaagagatcg tcaaagaccg taatggaaaa ccaatacggg taaaaaacag cggccaaaag
    44281 aaatatgtcg aatcggtagc caaacatgat atcgtatttg gcgtaggacc agccgggaca
    44341 ggaaaaactt ttcttgcagt agtattagca atcgctgcct tgaaaaaagg tgaagtgcaa
    44401 aaaatcattt tgacgcggcc tgctgtggaa gcaggggaaa atctaggctt tcttcccggg
    44461 gatctaaaag aaaaagtaga tccttatctg cgtcctgttt atgatgcttt ataccaaatt
    44521 tttggattag atcatacgaa ccgattgatg gaacgaggcg tgatcgaaat tgctccttta
    44581 gcttacatgc gggggcggac attggatgat gcattcgtta ttttggatga agcacagaac
    44641 acgacagttg ctcagatgaa aatgttcctg acacgtcttg gttatcaatc aaaaatgatt
    44701 gtcaatggag atacgagtca gattgatctg cctcgaggga caacaagtgg tcttgttcat
    44761 gcagaacgaa cattaaagca aatcaagaaa atcgattttg ttaattttga agcaagtgac
    44821 gttgttcggc accctgtagt tgccgaaatc atcaaagctt atgaaaaggc agatctccat
    44881 caagaataag ctttgcttag gaggataagc acatgttgaa taaaacactg cagaaactac
    44941 aagaaaaact tggaaaggca gcattgcctt tcctgctgtt cttgtttttt atcattatct
    45001 gcggcgttac atttagtagt gtcaaacaaa aggccaatga tttcagagaa ggccaagtag
    45061 cagaagaaag tatccgtgcg aacaaaacga tcgagaacac ggaagaaacc gaacaaaaaa
    45121 gaaaactggc agcagaagca gtcactccag aatacactta ccaaaaagat cttgcagatg
    45181 accagaacaa tcggatcaag caactatttg aattgatcga taaaacgaat gatgccatca
    45241 acaaatctta tgacgaaaag attgataagg caaaggaaga tgaaaatatc ccaaagccta
    45301 ctacagaaga gcggatcgcc gcgttgaaga aaaattttga aaatgtgaat gcagaaaatg
    45361 ttgcgtttta tcaaaaacta ccgaacaatt tttattcgac gatatttgaa atgactgaaa
    45421 ctgagatcca tactgtgagg gatgagagtc tgaagctcat tgacgaacag atgagcaaac
    45481 aagttcgtga aagtgaattg gaagctttta aacaagaagc agaagagcaa atccaatatt
    45541 tgaatgtcac accagcacaa caacaaatga ttcgctactt agtggatcaa ggtatcgtag
    45601 tgaatgacgt attgaatgag aaaaagacag aagaattgaa acaatccgca cgagaagctg
    45661 tacaaccagt gatgatcttc caaggagaaa tcattgttcg tgaaggtaat caaatcgatg
    45721 cttcagctat gaaaaaattg gaattactag gactgactaa tcaaacaact tcgatttttc
    45781 cgctaattgc catggtttta gctgttttgc ttcaaatcgg cgtactgatt tataattcac
    45841 ttcaattcca aaatgcagga gaaagaacaa aatacgtgct tttctatgtg acagctatgt
    45901 cagtcagcat agtgctgatg aaatttttcc aattattcca aacggaacaa gctacttata
    45961 tcccgctgtt ttatcccgct gcttttgcac cacttgtttt aagctttttc ttgaaccgaa
    46021 gagcagggat catggctggt attttccaag cagtatctgc acttttcatt ttttatgatt
    46081 cgattggcac gaactttcta acgattatct tgatgtctta tgttttctct gggctcatgg
    46141 caacgattgt cagacgcaaa cggatctcag aacaaggatt atctgctgcc atgtgggtca
    46201 ttatttttcc tgtgatgatg gatgtcattt tgatcgtcta tcaaggaatg agctttggtg
    46261 atgcaacgac ttggttaatg cttgtctgcg ggttcactgg tgcactcttc tcattcttgc
    46321 tgacgatcgg tctacaccca tatattgaat tgatggtcaa tgatgacagt gtaattgtat
    46381 taaatgaact cagcaatcca aatcatcctt tattgaaaca gttgctggaa gaagcgccag
    46441 gtacttatca tcatagtatg atggtagcta atctaagtgc taacgctgta gcagaaatcg
    46501 gcggacgttc tttgctaact cgagtagctt gctattatca tgatatcggc aagatcaaac
    46561 atgctagctt tttcgttgag aacttgcctt caggagcgga aaacccgcac aatttcttat
    46621 tgccagaaga cagtaaacat attatctttg gacatgtgac agatggagcc aaaatattgg
    46681 aagattataa tatgccgcaa atggtgatcg atatttgcaa acagcatcat ggaaccacgc
    46741 taatgcgtta tttctacatc aaagcgaaag aacgcaatcc ggaagtaaca gaagagcaat
    46801 tccgttatcc tggaccaaaa ccgcaaagtc gtgaagcagg gatcgtcaat attgctgaca
    46861 gttgtgaggc tgcagtacgg gcaatggatc atccgacgag cgaaaaaatc gaaagttttg
    46921 tccataatct tatcgaagag cgtatttccg atggtcaatt agacgatagt ggactgacct
    46981 tgaaagaaat ccgaaaagtg gaaaaatcat tgatcagcgg tttaagtagc accttccatt
    47041 ctcggatcaa atatcctaaa atgaaaacag aagttgagaa gataaaggaa gaacaagaag
    47101 aatagaaaga agcgatgtat aatggatatt acctttatgg atgaaacaaa ccaagtctca
    47161 gaagagaaaa tgaaagaaat cgatgacttg ctgcaatttg cagcatctta tttaaagctg
    47221 ccagaggaaa cagaaatgtc tgttactttc atggacaatg cggcaatcca agtaatcaat
    47281 cgtgattacc gtggaaaaga tgcaccgaca gacgtcatca gttttgctct ggaagaagaa
    47341 ggcgaagacg aaatcccagt gatttttgaa gatggagaga atccattgcc tcgtgtacta
    47401 ggagacatca tgatttctat tgaacgtgct gctgaacaag cagaagctta cggccattca
    47461 tatgatcggg aacttggctt tttagcggta catggattct tgcatatcaa cgggtatgac
    47521 catatgacac cagaagatga gaaagaaatg tttggtttac agaaagagat cttggatgcc
    47581 tatggactca aaagataaaa acgtagagaa aaacaaacat ttcctgatgt ctgtggaatt
    47641 tgctgtccaa ggagtcaaaa ccgtttttga ggaagaacgc aatatgagga aacatgttgc
    47701 gttcggtatc cttgctttat ttatgggcgt gattttccag cttgatcgga tagagtggtt
    47761 atggctgctt ttagctgttt ttctcgtttg gatagtagag atcatcaata ctgtttttga
    47821 aaatgtagtg gacatgttta cggattttca ttttcatccg attgggaaaa aaatcaaaga
    47881 tatggcagct ggagcagttt tagtgaccgc ttgttttgct gtcatcattg ggttgatttt
    47941 atttgtccct aaaatatatc atttgttgtt taattaaaaa ggagaacgta tatgccagaa
    48001 cataaatcag ggtttgtcgc aatcgtcggc cgcccgaatg taggaaaatc cacattattg
    48061 aaccgaatcg tgggtcaaaa aattgccatt atgagtgaca aggcacagac aactagaaat
    48121 aaaatccaag gagtatatac tacaccagaa gctcagctga tttttatcga tacaccagga
    48181 atccataaac caaagcatcg tctaggagat tttatggtag aagcagctta cagtgcgttg
    48241 aaagaagtgg atgcagtctt atttatgatc agtgctgatc aaaaacgcgg ccggggagac
    48301 gattttatta ttgaacgtct aaaaaacgtg cattctccag tttatctgat tatcaataaa
    48361 atcgataaga tccatccaga cgagttgctg ggaatcatcg aagattattc gacgcagatg
    48421 ccttttgcac aagtgattcc gatctctgct acagaaggaa acaatgtcga acgcttaatg
    48481 gatgtattag tcagtgaaat gccagagggc ccacaatatt ttccagatga tcaagtgact
    48541 gatcatcctg aatactttat cgtttctgaa ttgatccgtg agaaagtctt gtttttaaca
    48601 cgagacgaag tgcctcattc tgtcgcagtc gttgttgatt cgatgaagcg aaacgagaat
    48661 gataaaatcc atatccaggc cacgatcatc gtcgaaagag atagccaaaa aggaatcatt
    48721 atcggcaaag gcggaaaaat gctgaagcaa gtaggaacga aagcaagaaa ggatatcgaa
    48781 aatcttttag gagacaaagt atttctagaa ctttgggtca aagtccaaaa agactggcga
    48841 gacaaaaaag tctatctgca agactttggc taccgaaaag acgaatactg atattctgaa
    48901 cttcttatag gttaagtata gaaataaact ggataaaaat ccgcacgttg agaaaaacgg
    48961 cggaagatcg aaacataaat aatctggatc ggcagaatct gcttttccag atttattttt
    49021 taggagggag aatatgagcc agcttgcaga atcgaaaggc ttgattttat ctagccgtaa
    49081 ttacaaagaa aaagataaat tagtcaaaat ctttacggaa tcttctggca aaatgatgtt
    49141 ttatatcaaa ggaatccatc ggcaaaatca gccattagct ccggctttgc tcccctttac
    49201 cgaagcagtc tatatcggta attttcgaag tgaaggtttg tcgtttttaa gcagtgtcaa
    49261 agacgtccat gcgtttcgga acatccaaga agatatcttt ttgagcggtt atggtaccta
    49321 tttgctaaat ctagtagatg cagcaatcga agatcatcaa tatgacccgc atttattcca
    49381 atttacgcaa caagctctgc agcggatgga tcaaggagat gatgcagaaa tcatcacgaa
    49441 tatctttgaa gttcaaatac tgcaacgctt cggaatcgcg ccaatttgga cgcactgtgt
    49501 cgtatgcggg gaaacaaaag gaaaatttga ctattcttcc aaatctggcg gtgtgatatg
    49561 cgagaaacac tggccaatgg acgaacaccg ttatcatgca gatcctcgcg ctgtttattt
    49621 cgttcgaatg ttttcggcta tttcttatga taagatcagt ggaatccact tgaaagaaga
    49681 aacgaagcag gcaatccgtg acctcattga ccagttgtat gatgaatatg tcggtattca
    49741 tctaaaaagc aaaaagttca ttgatcaaat gaagtcttgg gcacacgtat taaagacgcc
    49801 agagaaaaat atgcctgaaa aagagatacc tgaaaaagaa gaataaatga aagatacaac
    49861 aaaaaaatca aaactatttg acaaatacag ggacttccgt ctattattaa ccgtagatta
    49921 aatagatcgt tcactgagag gatctattat ataaacaaga aaactgcaaa atgaatacgt
    49981 acattgacca agatcagtaa aaaacacgtg gtttagcgag ttcgggagag tggaagccgg
    50041 acccaaacga tttttgaaga ggcacttgcg agtacgataa atgaagctgc cgaatcttta
    50101 ttcggaagta gggtggaacc gcgaaattat tcgtccctat gtctgtaatc agacataggg
    50161 actttttgtc gttttctttc ctgtattctt attgacagca acgcttaaaa attgaaaggg
    50221 gcaaaacaga tgagtaaaaa attgaccgtc caagatatga ttttaacatt acaaaaattt
    50281 tggtcatcaa acggctgcat gctgatgcaa gcttatgaca cagaaaaagg agcaggtacg
    50341 atgagcccat atacattctt acgggcaatc ggaccggaac catggaatgc tgcttatgta
    50401 gagccatccc gccgaccagc tgacgggcgt tatggagaaa acccaaaccg tttgtatcag
    50461 catcatcaat ttcaagtcgt aatgaaacct tcacctgaaa atatccaaga gctttattta
    50521 gaaagcttgc gtcttttagg aattgatcca ttagaacacg atattcgttt tgttgaagac
    50581 aactgggaga acccatcgat ggggtgtgcc ggtgtgggtt gggaagtatg gcttgatggg
    50641 atggagatca cacaattcac gtacttccag caagtcggag gactaccttg taaaccagtt
    50701 acttctgaga tcacttatgg actagaacgt cttgcttcat atattcaaga agttgaaagt
    50761 gtctatgatc tggaatgggc tgatggtgtg aaatatggcg agatcttcaa acaaccagaa
    50821 tatgaacatt ccaaatattc ttttgaagta agtgaccaag acttgctact aagcaacttt
    50881 gaccgttttg aaaaagaagc taaacgctgt atcgatgaaa atcttgttca tccagcgtat
    50941 gattatattt tgaaatgcag ccatacattc aatttattag atgctcgcgg tgcagtatcc
    51001 gtaacagaac gagcaggtta tctttcccgt atccgcaaca tggctcgtgc tgtagccaaa
    51061 atctttgtgg cagaacgcga aaaattagga ttccctttat tgaataaaaa cacagcaaat
    51121 ccagaaaaca tgaaggaggc tgaataagat ggcaaaagac ttattacttg aaatcgggct
    51181 ggaagaaatg cctgctcatg ttgtcacacc aagcatgaaa caattagaag aaaaaacagc
    51241 aaaattttta gatgaaaacc aattgactta tgatacgatc gaaacgtttt ctacaccgcg
    51301 ccgcttagca atcaaagtaa caaacattcc agaacgtcaa gcagacagtg aagaagaagt
    51361 caaaggacca gccaaaaaaa ttgctttgga cgcagatgga aactggacga aagcagctga
    51421 aggatttgtc cgcggacaag gtctgacgac agatgctatc acttttagag aattaaaagg
    51481 cgtggaatat gtctatgtaa cgaaatacac tcacggcaaa gaagcgaaag aagtattgac
    51541 aggtttagca gatgtcatta aaagcctcac tttcccagta acgatgcatt gggcagaata
    51601 cgattttgaa tatatccgcc ctattcactg gatcgtggca ttgttagatg acgaagtaat
    51661 cccgtttgag atcttagatg tcaaaacagg cagagcctct cgaggacatc gtttcttagg
    51721 cgaagatgtg acatttgcgc atgcaaaaga atatgaagaa aaattgaatg aacaattcgt
    51781 tattgcttca ccgcaaaaaa gacaagaaat gatcgttaca caagcaaatg aattagccga
    51841 aaaaaatcac tgggttattg atctagacga agatttacta gaagaagtca acaatttagt
    51901 cgaatatcca actgtctttt ctggcgactt cgaagaaaaa tatctttctg ttcctgaaga
    51961 agtactagtc acttctatga aagaacatca acgttatttc gaggtacgga acgatcaagg
    52021 tatgctgctt cctcatttta tttctgctcg taatggtgat cgggtaaaat tggaaaatgt
    52081 ggttcgaggc aatcaaaaag tattgacggc tcgtttagaa gatgcggaat tcttttataa
    52141 tgaagacaag aaagcttcaa tcgaccaata cgttgaaaaa ctaaaaactg ttactttcca
    52201 tgaaaaaatc ggtagtatct atgagaaaat gcagcgggta caagtcattg cccgtatcat
    52261 tggcgaacaa gtaggactat ctgaggaaga attgatggat ctgcaacgag ctgctgaaat
    52321 ctataaattt gatctagtaa ccaacatggt aggggaattt cctgaactac aagggatcat
    52381 gggcgaaaaa tatgctctgc tgaaaggcga gaaacctgct gtagcaacag cgattcgtga
    52441 gcattacatg cctatatcca gtgaaggaga gctacctcag tctacagtag gttctgtttt
    52501 agcgattgca gataaattag atagcatttt ttctttcttt gcagttggga tgattccaag
    52561 cgggtcaaac gatccttatg ccttacgtcg ccaagcatac ggtgtcgttc ggatcatcga
    52621 atcgaaaaaa tgggcctttc ctttgtctgt tctgcaagaa acgattagtg atgttatcag
    52681 taaagatacg gatcgtttcg gcattggttt atcagaaggg caacaacagg tgatcgactt
    52741 tatcaaaggc cgtctgcgcc aattattgac aacgaagaat attcgtcatg atgtcatcga
    52801 agctgttttg aatgcagaac aaaaagatct gacaaaagta tttgcagcag cacaattgtt
    52861 caagcagcat cttgcagatg aagacttcaa accatcgatg gaagctttga cacgtgtcgt
    52921 gaacttagcg aaaaaagcgg agcttgaaaa acaatcagaa gtagaccctg aactgttcga
    52981 aaatgaagcg gagaaagaac tgcacaaagc agtagaggca atgaaaaaaa gctttgctga
    53041 ccaaacgatc aacgaaaatt atgatcagct tgtaagcctt cgcccactga tcgaaaatta
    53101 ttttgatcag acgatggtca tggcagaaga accagcagta cgtaagaacc gattaacgca
    53161 attaagtcaa attgctgcca tggctttgtc gctagcaagt ttagatcaaa tcatcacaaa
    53221 ataatttcta atatgtaaat aaaccagaga ataggcacaa aa
//
LOCUS       DACDID010000004        47039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDID010000004 DACDID010000000
VERSION     DACDID010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.004"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..2346
                     /locus_tag="IV236_000178"
     CDS             202..2346
                     /locus_tag="IV236_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8770405.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2398..3438)
                     /locus_tag="IV236_000179"
     CDS             complement(2398..3438)
                     /locus_tag="IV236_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8770406.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3715..5517)
                     /locus_tag="IV236_000180"
     CDS             complement(3715..5517)
                     /locus_tag="IV236_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770407.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5531..6283)
                     /locus_tag="IV236_000181"
     CDS             complement(5531..6283)
                     /locus_tag="IV236_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770408.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6292..6654)
                     /locus_tag="IV236_000182"
     CDS             complement(6292..6654)
                     /locus_tag="IV236_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8770409.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6830..7702)
                     /locus_tag="IV236_000183"
     CDS             complement(6830..7702)
                     /locus_tag="IV236_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8770410.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7728..8240)
                     /locus_tag="IV236_000184"
     CDS             complement(7728..8240)
                     /locus_tag="IV236_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8770411.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(8378..9208)
                     /locus_tag="IV236_000185"
     CDS             complement(8378..9208)
                     /locus_tag="IV236_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8770412.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(9224..9832)
                     /locus_tag="IV236_000186"
     CDS             complement(9224..9832)
                     /locus_tag="IV236_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8770413.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9825..10142)
                     /locus_tag="IV236_000187"
     CDS             complement(9825..10142)
                     /locus_tag="IV236_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8770414.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10298..11026)
                     /locus_tag="IV236_000188"
     CDS             complement(10298..11026)
                     /locus_tag="IV236_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8770415.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11185..12066
                     /locus_tag="IV236_000189"
     CDS             11185..12066
                     /locus_tag="IV236_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8770416.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12303..13346)
                     /locus_tag="IV236_000190"
     CDS             complement(12303..13346)
                     /locus_tag="IV236_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8770417.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13349..14017)
                     /locus_tag="IV236_000191"
     CDS             complement(13349..14017)
                     /locus_tag="IV236_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8770418.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14159..16495
                     /locus_tag="IV236_000192"
     CDS             14159..16495
                     /locus_tag="IV236_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770419.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16610..16921)
                     /locus_tag="IV236_000193"
     CDS             complement(16610..16921)
                     /locus_tag="IV236_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770420.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17102..18577
                     /locus_tag="IV236_000194"
     CDS             17102..18577
                     /locus_tag="IV236_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8770421.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18624..19208
                     /locus_tag="IV236_000195"
     CDS             18624..19208
                     /locus_tag="IV236_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8770422.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            19210..19629
                     /locus_tag="IV236_000196"
     CDS             19210..19629
                     /locus_tag="IV236_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8770423.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(19910..20194)
                     /locus_tag="IV236_000197"
     CDS             complement(19910..20194)
                     /locus_tag="IV236_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8770424.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20546..21256)
                     /locus_tag="IV236_000198"
     CDS             complement(20546..21256)
                     /locus_tag="IV236_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8770425.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21253..22725)
                     /locus_tag="IV236_000199"
     CDS             complement(21253..22725)
                     /locus_tag="IV236_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8770426.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22863..23336
                     /locus_tag="IV236_000200"
     CDS             22863..23336
                     /locus_tag="IV236_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8770427.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23489..24040)
                     /locus_tag="IV236_000201"
     CDS             complement(23489..24040)
                     /locus_tag="IV236_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8770428.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24145..24675
                     /locus_tag="IV236_000202"
     CDS             24145..24675
                     /locus_tag="IV236_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8770429.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25158..25601)
                     /locus_tag="IV236_000203"
     CDS             complement(25158..25601)
                     /locus_tag="IV236_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770430.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25617..26777)
                     /locus_tag="IV236_000204"
     CDS             complement(25617..26777)
                     /locus_tag="IV236_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8770431.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27284..28984
                     /locus_tag="IV236_000205"
     CDS             27284..28984
                     /locus_tag="IV236_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770432.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(29086..29919)
                     /locus_tag="IV236_000206"
     CDS             complement(29086..29919)
                     /locus_tag="IV236_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8770433.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30223..32388)
                     /locus_tag="IV236_000207"
     CDS             complement(30223..32388)
                     /locus_tag="IV236_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770434.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32648..34942
                     /locus_tag="IV236_000208"
     CDS             32648..34942
                     /locus_tag="IV236_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8770435.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34935..35600
                     /gene="pgmB"
                     /locus_tag="IV236_000209"
     CDS             34935..35600
                     /gene="pgmB"
                     /locus_tag="IV236_000209"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8770436.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            35593..36600
                     /locus_tag="IV236_000210"
     CDS             35593..36600
                     /locus_tag="IV236_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8770437.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36622..37641
                     /locus_tag="IV236_000211"
     CDS             36622..37641
                     /locus_tag="IV236_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8770438.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37922..39289)
                     /gene="nhaC"
                     /locus_tag="IV236_000212"
     CDS             complement(37922..39289)
                     /gene="nhaC"
                     /locus_tag="IV236_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8770439.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39399..40820)
                     /locus_tag="IV236_000213"
     CDS             complement(39399..40820)
                     /locus_tag="IV236_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8770440.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(41063..42940)
                     /gene="tdc"
                     /locus_tag="IV236_000214"
     CDS             complement(41063..42940)
                     /gene="tdc"
                     /locus_tag="IV236_000214"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8770441.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43231..44487)
                     /locus_tag="IV236_000215"
     CDS             complement(43231..44487)
                     /locus_tag="IV236_000215"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8770442.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            45067..46368
                     /locus_tag="IV236_000216"
     CDS             45067..46368
                     /locus_tag="IV236_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8770443.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46437..47036)
                     /locus_tag="IV236_000217"
     CDS             complement(46437..47036)
                     /locus_tag="IV236_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8770444.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        14422 a         9712 c         8363 g        14542 t
ORIGIN      
        1 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
       61 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
      121 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
      181 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
      241 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
      301 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
      361 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
      421 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
      481 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
      541 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
      601 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
      661 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
      721 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
      781 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
      841 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
      901 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
      961 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     1021 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     1081 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     1141 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     1201 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     1261 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     1321 gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     1381 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     1441 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     1501 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     1561 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     1621 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     1681 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
     1741 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
     1801 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
     1861 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
     1921 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
     1981 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
     2041 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
     2101 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
     2161 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
     2221 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
     2281 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
     2341 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
     2401 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
     2461 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
     2521 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
     2581 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
     2641 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
     2701 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
     2761 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
     2821 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
     2881 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
     2941 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
     3001 tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag tctgatagaa
     3061 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
     3121 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
     3181 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
     3241 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
     3301 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
     3361 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
     3421 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt
     3481 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
     3541 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt
     3601 ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     3661 atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa
     3721 tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt agatgaaata
     3781 caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt gcatgaatag
     3841 accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg gtgctaagaa
     3901 taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg ttcctatctt
     3961 ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct tgaacatcaa
     4021 acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa attcaaaacc
     4081 agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt actctacttt
     4141 ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg gtttcaggtt
     4201 tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt tcagttgatc
     4261 aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca attcatagct
     4321 tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt tttctttcgt
     4381 gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag ccgcaaattc
     4441 acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag cagaaagtgt
     4501 tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca tcactagatc
     4561 atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta atcctaccgt
     4621 caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt gtgtatattc
     4681 acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca atgaaatctg
     4741 ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa tgaaataagt
     4801 tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg tccccacgtg
     4861 tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt caaggaaaaa
     4921 caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca atgctaaaat
     4981 cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca aaacagcaaa
     5041 aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg aaatattgag
     5101 atctaactga tccactaata atcggtggac gatacttgtc aatcctacac cgacagctga
     5161 acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca tctgtgcgat
     5221 ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc gcatcgtcat
     5281 caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta aaacagaacc
     5341 gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa aagcttcgtt
     5401 gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc ctgagaataa
     5461 caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc aaagcatagt
     5521 caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga tagaaggaat
     5581 cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg atgaataaaa
     5641 tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc gatacatgat
     5701 cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg gaatccagcg
     5761 cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca cgtgcggcgg
     5821 cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa atatgagaga
     5881 tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt ctgacgcctt
     5941 gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat aaattgaaat
     6001 cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt ccttttaatg
     6061 tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct aatgtggata
     6121 aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca ataaattcgc
     6181 ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca ttgttttttc
     6241 cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc cctacccttt
     6301 aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt catacgaagt
     6361 gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa gcggtcgagt
     6421 caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc caggcaggat
     6481 atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt ttgtaccatc
     6541 tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg cgattttcac
     6601 ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt tcactttttc
     6661 cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct gccttcgaat
     6721 caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa aaagagttca
     6781 agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt cacttacggt
     6841 ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg attgagacaa
     6901 gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct tgtccatctt
     6961 cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg gtttgtcgag
     7021 cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt tctgttgcat
     7081 aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct aaacgctctt
     7141 gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc gtcaaatcat
     7201 tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct gtctcacttt
     7261 ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct gtatcaacga
     7321 ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca ttgactttta
     7381 aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt gccaaaggta
     7441 tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa aacaaaatta
     7501 cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac gtgatcaacc
     7561 aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct aaatagaaga
     7621 aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa ccattgatat
     7681 aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca ataaatatct
     7741 aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag ttctaactct
     7801 ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg cggtaaataa
     7861 tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac caagacctct
     7921 agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat aaatcgttcc
     7981 ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata ataatccagc
     8041 tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc taattggcga
     8101 ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc atagaaggtt
     8161 tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa cttttctctt
     8221 tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat attttctttc
     8281 ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg gaaggatctt
     8341 tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca ttgtcctctt
     8401 gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt ttctgtcctt
     8461 gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat tcaccagacg
     8521 tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca ccgtttagga
     8581 tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg ttttcacctg
     8641 tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat tcatctttcg
     8701 cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt gtcttagtat
     8761 tcactttttt gattgatact tcgttgatat caattgcact attattccct tcgattgtca
     8821 attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt gtgtcttcta
     8881 aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg ccatttttct
     8941 cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa tccaatccgc
     9001 gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca tccttgattt
     9061 tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc agactctttt
     9121 gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc agaccaatac
     9181 cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg cccgctgaat
     9241 gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc atcatgacat
     9301 aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg cttcctgtgg
     9361 cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca aacacactga
     9421 ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa acatagatac
     9481 taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata aaccccagtg
     9541 ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa atcaggcgaa
     9601 tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct gaaaagtaac
     9661 tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg atctgatcta
     9721 atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca aaacgatcgt
     9781 cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc atttgtgctc
     9841 tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat aatttcttct
     9901 aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc ttcaaaaggt
     9961 tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg atatagtgta
    10021 gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc atacatatct
    10081 tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat ctggatgtgc
    10141 attacgtcac cttctattct caagtgtaat attttatttc aatctatatt atacaacata
    10201 taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag atttgatcct
    10261 ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc ataatccaat
    10321 ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg tcagctactg
    10381 ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat ccgccgcaag
    10441 ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt gccaataaga
    10501 acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca taccctaaat
    10561 tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc aaaatcgtac
    10621 tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt gcacaaaata
    10681 gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca agcttttcac
    10741 tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt atcccattac
    10801 ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata acagccggca
    10861 tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca ccaaaaacca
    10921 tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg ccaattagtc
    10981 cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa aaactcctct
    11041 ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt tcgctgcaag
    11101 aaagattgta acagacagag aagattacac caattcattt tttgtgttaa agtattcttt
    11161 attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt tcttgattta
    11221 gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca aggaaatatt
    11281 tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga acgcgcccat
    11341 agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa aaatgtcgta
    11401 gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa caatctttca
    11461 ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa aatcaccgag
    11521 tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag tgtcgaatta
    11581 acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa tttccaggcc
    11641 ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc agttctacct
    11701 aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac
    11761 tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct gtgtcgcgaa
    11821 gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat catcaatatg
    11881 attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa
    11941 gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc atttactcgt
    12001 aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa tcaagaagaa
    12061 aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg acgcatgact
    12121 ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat ttctcgtagt
    12181 tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc cctaatcaca
    12241 aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt ttattctttt
    12301 tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat actgctataa
    12361 tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc tagcccattc
    12421 ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg atctctggtc
    12481 tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt ggctgtctca
    12541 aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt atccatgata
    12601 ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc agcaagttcg
    12661 atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat tacttctctg
    12721 atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt ttccagtttg
    12781 ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc gataatccga
    12841 tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa ttgaacatat
    12901 ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac aaagtcattc
    12961 ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt atacagttgc
    13021 tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc
    13081 agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc aacaaaaatc
    13141 gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg gtataacgtt
    13201 ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa aataaaaccg
    13261 agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa atagatccat
    13321 aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct agtccccgta
    13381 ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt ttgatatgta
    13441 catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc atcaactgtt
    13501 ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga aattcttttt
    13561 gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc tctaattttg
    13621 tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc aatgcttcca
    13681 cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct gctcctaatt
    13741 tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt
    13801 caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc ggcatcatga
    13861 tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca tcctctccat
    13921 tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa agcaactgta
    13981 attgcggatc atcttctact actaaaatac gattcattga tctccctctg ttttttgaaa
    14041 aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt atactacgat
    14101 cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa gggtgattat
    14161 gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt
    14221 ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg
    14281 ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga
    14341 tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg
    14401 aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat
    14461 tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg
    14521 ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa agccaaatca
    14581 gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga
    14641 tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat
    14701 ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga
    14761 actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga tcttaacaga
    14821 ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat
    14881 gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac
    14941 tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct
    15001 ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc
    15061 gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag
    15121 caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc
    15181 acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa
    15241 cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa
    15301 tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt
    15361 atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga
    15421 cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc
    15481 ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt
    15541 gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg
    15601 tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt
    15661 tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc
    15721 ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta
    15781 tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc
    15841 tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca
    15901 aacattgctt agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa
    15961 caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc
    16021 agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg
    16081 attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag
    16141 tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg
    16201 tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac
    16261 gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt
    16321 ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc
    16381 tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct
    16441 tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg
    16501 tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa
    16561 cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg
    16621 gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta
    16681 tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa
    16741 gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta
    16801 tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca
    16861 aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc atttttttca
    16921 ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta
    16981 ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat
    17041 tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct
    17101 catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg
    17161 tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg
    17221 ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt
    17281 caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa
    17341 acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc
    17401 tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt
    17461 cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa
    17521 tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga
    17581 aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg
    17641 tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt
    17701 gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac
    17761 gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt
    17821 gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg
    17881 tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa
    17941 cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc
    18001 tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac
    18061 aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat
    18121 tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc
    18181 aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg
    18241 tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat
    18301 tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac
    18361 tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga
    18421 cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga tgagtgcaaa
    18481 aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt
    18541 ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga
    18601 catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga
    18661 ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag
    18721 aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc
    18781 tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca
    18841 aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac
    18901 cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac
    18961 gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc
    19021 cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca
    19081 taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc
    19141 atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa cgttggcagg
    19201 gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa
    19261 gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca
    19321 catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct
    19381 agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc
    19441 aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc
    19501 aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat
    19561 tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa
    19621 acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat
    19681 ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga
    19741 acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt
    19801 gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt
    19861 gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct
    19921 tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc
    19981 gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac
    20041 tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt
    20101 cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact tcggaagcat
    20161 agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta
    20221 gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt
    20281 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat
    20341 gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc
    20401 tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    20461 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    20521 aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact aattttgcga
    20581 atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc
    20641 tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa gcttttgctt
    20701 cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct gcttggtccg
    20761 tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg tcatattcgt
    20821 gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat tcccaataag
    20881 tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt gtttcgttat
    20941 ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga tacacttttt
    21001 cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa tcagcactgg
    21061 aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga ttggacaaat
    21121 tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa gataaaaagg
    21181 tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca
    21241 aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt ttttcttcaa
    21301 taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa acgcggcatg
    21361 gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg tattatccaa
    21421 tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga tcaaaatagc
    21481 tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc catgactgat
    21541 caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga ccatggagat
    21601 cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg tttttgtcaa
    21661 gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt
    21721 cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca tgatcaatgc
    21781 acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat tttgaatata
    21841 caatggtacg accatttctg cccctaccat cgccatattt gttacaccag ctaaaacagc
    21901 tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat gttccaaatg
    21961 taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga aaccaattac
    22021 tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta aactaccgaa
    22081 tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca atggaataac
    22141 acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca gtaccatacc
    22201 aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag ggcctaaagc
    22261 aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt tttctggcgg
    22321 gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc cgcaagcttg
    22381 gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag taatcgttcc
    22441 gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt tattgatcag
    22501 ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg ttaaccattg
    22561 cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa
    22621 ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta aaagtgtccg
    22681 attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc tccttctttc
    22741 tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt gtcaacctac
    22801 ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa ggagtgacaa
    22861 aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt gttggcatca
    22921 gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg
    22981 gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt ttacctacag
    23041 tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg tcaaaagctg
    23101 cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag
    23161 caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc ggtaacttca
    23221 aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa ttaacttaca
    23281 agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa ctctaaacgc
    23341 cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat acttgaaaaa
    23401 aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca tatagaaagg
    23461 aattctccat cactgttcgg atattttttt atttttttcg gttactttct tctaatacat
    23521 ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa atcaggtagt
    23581 gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt cgctctaata
    23641 gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct actaatgccg
    23701 gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta
    23761 gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct tctgttaatt
    23821 cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga atgattcctt
    23881 gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc caaaattctc
    23941 ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc ttacttaata
    24001 accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc tccttgacaa
    24061 atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta ttacatgttt
    24121 taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga ttttattgga
    24181 actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc caaaaatgac
    24241 catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt aaaagaccaa
    24301 gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac agagccaaca
    24361 gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg aatgatcttt
    24421 ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa cgaacatatc
    24481 gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact tgttcctgaa
    24541 tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt tattagcgaa
    24601 gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga tgaaaattat
    24661 aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg acctgagtat
    24721 tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc taataccgag
    24781 ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat gctttgagta
    24841 ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg cttattaccg
    24901 agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag
    24961 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    25021 gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata ttcacacagt
    25081 taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct gggagcatta
    25141 tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac aagttcctcc
    25201 tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa
    25261 tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt ggtttctttt
    25321 atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt cacaaagatt
    25381 cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc tatttctgct
    25441 aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt ctcccaataa
    25501 gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga tagaacagat
    25561 tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct ccttacttat
    25621 tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt tcgcccctca
    25681 aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc aactttagga
    25741 tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt gactttccca
    25801 tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt tttcttatgt
    25861 ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat aataaatgaa
    25921 aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat aaacactaac
    25981 agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat ggttctttcc
    26041 tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg atgtcctgtc
    26101 actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt aactaagtct
    26161 tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg tatattttta
    26221 acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa
    26281 cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa
    26341 tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc tccattttca
    26401 aggatcctac cattgagccg atcataaagt gaaatattta attggatctt aggtatattg
    26461 atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt tctgttcttt
    26521 tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg ttttttctcc
    26581 tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac ttgttcgata
    26641 cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa agcgatcagt
    26701 aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg agactgtttt
    26761 tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt cttttattat
    26821 atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc
    26881 atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg ggttgggctt
    26941 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta
    27001 tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct aggctagtga
    27061 gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac
    27121 tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg
    27181 tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt
    27241 tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa
    27301 agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat
    27361 tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc
    27421 cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca
    27481 ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg
    27541 cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta
    27601 tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact
    27661 gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac
    27721 gtattctatt caatactggt ctatcaacca aggattcact agctggggga cttgttttta
    27781 tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa
    27841 agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc
    27901 tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc
    27961 actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc
    28021 aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat
    28081 gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc
    28141 tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt
    28201 ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc
    28261 atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg
    28321 atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg
    28381 gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca
    28441 gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg
    28501 acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat
    28561 gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga
    28621 gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt
    28681 tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac
    28741 tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt
    28801 gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa aaggaatcaa
    28861 cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt
    28921 gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc
    28981 ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg
    29041 agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc
    29101 taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat
    29161 ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt
    29221 ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc
    29281 attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc
    29341 ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa
    29401 aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca
    29461 agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa
    29521 gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa ggacctgact
    29581 ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg
    29641 gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg
    29701 gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga acttcattag
    29761 cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta aagcaatcac
    29821 ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg
    29881 ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat
    29941 ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct
    30001 cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt
    30061 ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc
    30121 tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg
    30181 ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc
    30241 gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc
    30301 gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc
    30361 catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa
    30421 agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc
    30481 tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat
    30541 cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg
    30601 tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac
    30661 tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac
    30721 catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa
    30781 gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat
    30841 cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac
    30901 gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg
    30961 aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc
    31021 ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat
    31081 caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt
    31141 gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat
    31201 attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg
    31261 gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg
    31321 ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct
    31381 tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc
    31441 tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa
    31501 ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa
    31561 ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata
    31621 cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg
    31681 caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg
    31741 tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac
    31801 agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt
    31861 tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc
    31921 cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc
    31981 ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt
    32041 aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg
    32101 attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca
    32161 acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat
    32221 attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat
    32281 tgatttccct atactgatca tcaacccagc tgccggcata actgctacca caaccattaa
    32341 agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc
    32401 ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct
    32461 ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc
    32521 tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg
    32581 tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag
    32641 gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac
    32701 acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta
    32761 tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta
    32821 tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc
    32881 agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa
    32941 cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg aattagatat
    33001 gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata aagtacgcat
    33061 ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat
    33121 agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca
    33181 aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga
    33241 agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac
    33301 tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga
    33361 agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc
    33421 agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga
    33481 aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca
    33541 aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga
    33601 agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga
    33661 tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta
    33721 ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac
    33781 taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca
    33841 acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa
    33901 cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta
    33961 caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt
    34021 agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata
    34081 catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta
    34141 tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca
    34201 aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga
    34261 gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt
    34321 agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca
    34381 ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg
    34441 gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta
    34501 tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc
    34561 agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt
    34621 ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg
    34681 gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc
    34741 accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt
    34801 attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag gttttcctct
    34861 agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag
    34921 aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata
    34981 ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg
    35041 accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt
    35101 tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa
    35161 gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg
    35221 gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg
    35281 caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta
    35341 ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag
    35401 caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta
    35461 tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg
    35521 gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa
    35581 agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata
    35641 aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc
    35701 ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac
    35761 gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta
    35821 gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca
    35881 caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc
    35941 aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca
    36001 ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc
    36061 tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt
    36121 tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg
    36181 cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca
    36241 atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta
    36301 gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag
    36361 aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt attattttct
    36421 acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga
    36481 ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc
    36541 aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa
    36601 caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt
    36661 gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca
    36721 aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc
    36781 aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa
    36841 gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg
    36901 aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac
    36961 gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt
    37021 caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag
    37081 ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa
    37141 caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg
    37201 actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct
    37261 ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag
    37321 aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt
    37381 cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact ctccgtcatt
    37441 agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc
    37501 gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa
    37561 ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt
    37621 cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg
    37681 gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga
    37741 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    37801 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc
    37861 ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc
    37921 ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg
    37981 caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc atcgttccca
    38041 taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg
    38101 ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg
    38161 tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg attccgatac
    38221 agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt
    38281 gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta
    38341 aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta
    38401 gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga
    38461 ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta
    38521 atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca
    38581 atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag
    38641 ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga
    38701 atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa
    38761 tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac
    38821 cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca
    38881 aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa
    38941 ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat
    39001 aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca
    39061 aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga
    39121 tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa
    39181 ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca
    39241 acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc
    39301 tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta
    39361 aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc taaacttttt
    39421 tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac
    39481 atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta
    39541 gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg
    39601 tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta
    39661 agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt agcatttgta
    39721 agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa
    39781 ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg
    39841 aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt
    39901 ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata
    39961 tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata
    40021 tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca
    40081 ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg
    40141 tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata
    40201 ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa
    40261 aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa
    40321 acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact
    40381 tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa
    40441 cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat
    40501 ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat
    40561 gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct
    40621 tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac
    40681 aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata
    40741 ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta
    40801 cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg
    40861 tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt
    40921 gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct
    40981 taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg
    41041 ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga
    41101 ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt
    41161 cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac
    41221 ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat
    41281 agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt
    41341 tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat
    41401 cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat
    41461 gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac
    41521 gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta
    41581 agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga
    41641 tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt
    41701 aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa
    41761 catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg
    41821 cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat
    41881 gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac
    41941 ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta
    42001 atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc
    42061 ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt
    42121 tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt
    42181 ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa
    42241 ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt
    42301 cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca
    42361 ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg
    42421 gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag
    42481 caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt
    42541 cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata
    42601 gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc
    42661 agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg
    42721 atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg
    42781 gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca
    42841 ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata
    42901 gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa
    42961 atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt
    43021 tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg
    43081 aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta
    43141 acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt
    43201 ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt
    43261 tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac
    43321 aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc
    43381 acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc
    43441 aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc
    43501 atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa
    43561 gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg
    43621 tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa
    43681 agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta
    43741 gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc
    43801 gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctacttc
    43861 tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc
    43921 tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg
    43981 cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat
    44041 atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag
    44101 taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc
    44161 aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt
    44221 ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc
    44281 aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat
    44341 caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct
    44401 cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg
    44461 ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag
    44521 caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg aaataaaaaa
    44581 agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct
    44641 agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac
    44701 tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga
    44761 aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc
    44821 tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc
    44881 attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa
    44941 gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct
    45001 tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg
    45061 aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt
    45121 attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt
    45181 cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt
    45241 actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa
    45301 aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag
    45361 aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc
    45421 ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat
    45481 atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt
    45541 ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc ctttaacggg
    45601 cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt cagctattat
    45661 gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa caatgaaatc
    45721 aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat ggttcctctg
    45781 atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa aggtaccttg
    45841 gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca atatggagac
    45901 aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa
    45961 gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca agcttcacta
    46021 gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa
    46081 acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc ttattcagca
    46141 gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg taatgtcgat
    46201 gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc tcggatcaat
    46261 atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct tgatccattc
    46321 atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt
    46381 tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt tatgctttat
    46441 ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt atatggaagc
    46501 agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa atagaggatc
    46561 aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg atggaaaaga
    46621 tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt
    46681 ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt cactcgattg
    46741 agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg aagattttcc
    46801 gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa tacttcataa
    46861 atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac ctgagcgttc
    46921 tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat caagtcaaaa
    46981 gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc gttcatttg
//
LOCUS       DACDID010000005        43866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDID010000005 DACDID010000000
VERSION     DACDID010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.005"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(125..469)
                     /locus_tag="IV236_000218"
     CDS             complement(125..469)
                     /locus_tag="IV236_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8770445.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(462..896)
                     /locus_tag="IV236_000219"
     CDS             complement(462..896)
                     /locus_tag="IV236_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8770446.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1034..1771
                     /locus_tag="IV236_000220"
     CDS             1034..1771
                     /locus_tag="IV236_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770447.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1764..2975
                     /locus_tag="IV236_000221"
     CDS             1764..2975
                     /locus_tag="IV236_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770448.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3182..3961
                     /locus_tag="IV236_000222"
     CDS             3182..3961
                     /locus_tag="IV236_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8770449.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4031..4693
                     /gene="trmB"
                     /locus_tag="IV236_000223"
     CDS             4031..4693
                     /gene="trmB"
                     /locus_tag="IV236_000223"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8770450.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4791..6545)
                     /gene="ade"
                     /locus_tag="IV236_000224"
     CDS             complement(4791..6545)
                     /gene="ade"
                     /locus_tag="IV236_000224"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8770451.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6677..7606
                     /gene="cpaF"
                     /locus_tag="IV236_000225"
     CDS             6677..7606
                     /gene="cpaF"
                     /locus_tag="IV236_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8770452.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7563..8612
                     /locus_tag="IV236_000226"
     CDS             7563..8612
                     /locus_tag="IV236_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8770453.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8609..8887
                     /locus_tag="IV236_000227"
     CDS             8609..8887
                     /locus_tag="IV236_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8770454.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8862..9332
                     /locus_tag="IV236_000228"
     CDS             8862..9332
                     /locus_tag="IV236_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8770455.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9349..9651
                     /locus_tag="IV236_000229"
     CDS             9349..9651
                     /locus_tag="IV236_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770456.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9668..10039
                     /locus_tag="IV236_000230"
     CDS             9668..10039
                     /locus_tag="IV236_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8770457.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            10011..10370
                     /locus_tag="IV236_000231"
     CDS             10011..10370
                     /locus_tag="IV236_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770458.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10542..11549
                     /locus_tag="IV236_000232"
     CDS             10542..11549
                     /locus_tag="IV236_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8770459.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11574..12758
                     /locus_tag="IV236_000233"
     CDS             11574..12758
                     /locus_tag="IV236_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8770460.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13195..14778)
                     /locus_tag="IV236_000234"
     CDS             complement(13195..14778)
                     /locus_tag="IV236_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8770461.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15356..15499)
                     /locus_tag="IV236_000235"
     CDS             complement(15356..15499)
                     /locus_tag="IV236_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8770462.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15721..16200)
                     /locus_tag="IV236_000236"
     CDS             complement(15721..16200)
                     /locus_tag="IV236_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8770463.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16273..16557)
                     /locus_tag="IV236_000237"
     CDS             complement(16273..16557)
                     /locus_tag="IV236_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770464.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16635..16829)
                     /gene="ssrS"
                     /locus_tag="IV236_000238"
     ncRNA           complement(16635..16829)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV236_000238"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16901..18187)
                     /locus_tag="IV236_000239"
     CDS             complement(16901..18187)
                     /locus_tag="IV236_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8770465.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18325..18996
                     /locus_tag="IV236_000240"
     CDS             18325..18996
                     /locus_tag="IV236_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8770466.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18962..19450
                     /locus_tag="IV236_000241"
     CDS             18962..19450
                     /locus_tag="IV236_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8770467.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19463..20410
                     /gene="prmA"
                     /locus_tag="IV236_000242"
     CDS             19463..20410
                     /gene="prmA"
                     /locus_tag="IV236_000242"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8770468.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20411..21175
                     /locus_tag="IV236_000243"
     CDS             20411..21175
                     /locus_tag="IV236_000243"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8770469.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21408..23621
                     /locus_tag="IV236_000244"
     CDS             21408..23621
                     /locus_tag="IV236_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8770470.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23632..24078
                     /locus_tag="IV236_000245"
     CDS             23632..24078
                     /locus_tag="IV236_000245"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8770471.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24249..25508
                     /locus_tag="IV236_000246"
     CDS             24249..25508
                     /locus_tag="IV236_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8770472.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25597..26544)
                     /locus_tag="IV236_000247"
     CDS             complement(25597..26544)
                     /locus_tag="IV236_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8770473.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26938..28230
                     /locus_tag="IV236_000248"
     CDS             26938..28230
                     /locus_tag="IV236_000248"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8770474.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28245..30014
                     /gene="aspS"
                     /locus_tag="IV236_000249"
     CDS             28245..30014
                     /gene="aspS"
                     /locus_tag="IV236_000249"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8770475.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30123..30998
                     /locus_tag="IV236_000250"
     CDS             30123..30998
                     /locus_tag="IV236_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8770476.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31264..33147
                     /locus_tag="IV236_000251"
     CDS             31264..33147
                     /locus_tag="IV236_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8770477.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33222..33506)
                     /locus_tag="IV236_000252"
     CDS             complement(33222..33506)
                     /locus_tag="IV236_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8770478.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33496..34230)
                     /locus_tag="IV236_000253"
     CDS             complement(33496..34230)
                     /locus_tag="IV236_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8770479.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34359..34982
                     /locus_tag="IV236_000254"
     CDS             34359..34982
                     /locus_tag="IV236_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8770480.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35174..36295
                     /locus_tag="IV236_000255"
     CDS             35174..36295
                     /locus_tag="IV236_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8770481.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36302..39430
                     /locus_tag="IV236_000256"
     CDS             36302..39430
                     /locus_tag="IV236_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8770482.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39449..42643
                     /locus_tag="IV236_000257"
     CDS             39449..42643
                     /locus_tag="IV236_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8770483.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42789..43496)
                     /locus_tag="IV236_000258"
     CDS             complement(42789..43496)
                     /locus_tag="IV236_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8770484.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14157 a         7781 c         9244 g        12684 t
ORIGIN      
        1 cccatttcct ttaaaaacgt tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca
       61 ttcctaggaa atacgcaaac ttttctcact tatatagtta aatctgttga gaaaataatc
      121 attattatat ttgttcagga tactctggca gatcagcttt tagtttttct acttgttcat
      181 tgatttgtgt gatacgtggt tcggcttgga atttaaaatc ttcgatatct tttttgattg
      241 cttcttggaa agcaggaatc acagattcag ctgtttcttt tgtttcgatc aatgcgcttt
      301 taaaatgatt caggctgtta tttaaatcat ttgtcagagt tgttgcttct tccaattctt
      361 cgatcagttt ctttcttgtt tcattcccgc tcctgggcgc caataacaga cctccgacag
      421 tgccagcagc agctccaaat aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc
      481 tgctttacga ttgtttcttg aatagctttt agttcttccg atccataact atcttgatga
      541 ttgacaaagt gaatagaaaa atcatcctct ttgctgtagc gagggactaa atggatatgt
      601 gaatgaaaaa cagattgata ggctagttca cggttgttat tcaagatatt aagacctttc
      661 atttctggaa aagctttttc caatgcttga gcaatcttag gcacacgcga aaaaacctct
      721 cctgcaagtt cgggatcata ttcaaaaata tctgctacat gacgtttagg cacaactaat
      781 gtgtgtcctt tcgttgcttg agagatgtct aaaaaagcat agaccttgtc gtcttcatat
      841 attttatagc ttgggatttc ttgattgatg attttgcaaa aaatacagtt ttccatataa
      901 aacatccttt ctataaagaa taaatacttc agcttaactt taccataagt taaagctatt
      961 gtaaggaaaa aaacaaaata ccgtcatttt cttcgtggta taataaataa aaataaatgg
     1021 cggaggaaaa agaatgagtt taattatcga tcatgtcaca ggggggtacg gacatctccc
     1081 tgtgttaaaa gatatcagtt tcgatgtaca gcctggagag atagtagggt tgattggact
     1141 gaacggagca gggaaaagta cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa
     1201 aggaacgata gaaatcaatc atctatcctt aaagaaaaat acggaagaat atcgcaaaca
     1261 aattgggtat attccagaga ctccttcttt atatgaagaa ttgacattga gagaacatat
     1321 cgaagtcact gcgatggcct acgatatacc tatggatata gcaatgaaaa gagcagaaaa
     1381 gttgttggag acttttcggt tatctaatcg tcttgactgg ttcccagcta atttttcaaa
     1441 gggaatgaaa cagaaagtga tgatcttatg cgcctttttg atcgagcctt ctttatatat
     1501 catcgacgaa ccatttttag gcttggatcc actagctatc aatgcattgc ttgaattgat
     1561 ggtagaaatg aaaaaagaag gagccggtat tttgatgtct acgcatattt tagctactgc
     1621 agaaaaatat tgtgatcgtt ttgtggtctt acataacgga gagatacgag cagacggttc
     1681 tcttgagatg ttgagaaaag aatttcagct gccggattct tctttggacg aaatctatat
     1741 tgctttaaca aaggagcagg cacatgagta gtttttttca aaaacgttta gcaagacatc
     1801 aaaagaaaat gatgaaatat atgcgttatg tattgaacga tcattttgtt ttagtttgtc
     1861 ttttcttgat tggcggtgtg ggattatatt attctaattg gttgaaaacc ctgcagccac
     1921 cgtttgcaat tggagggctc ataatctctg tattttggat gctctgttta ttcgtgggta
     1981 agatggctac ttttgcagag gcagcagata ttgttttttt acttcctaaa gaaaaagaaa
     2041 tgcaaaccta tttggaacga gcttttcgct attcttgcat ttttccgttg atctttttga
     2101 tattggcatg cggattggcc atgccattag tggtggttag tacggagcaa tccttttcga
     2161 tgtttttcgt ctatctgatc atattatgga gtttgaaatt cagtcatctg caaatcaaga
     2221 gagcagaatt gtttcgacta gatcaacaag ttattcgtta ttggaaatta ggctggtttt
     2281 tttcttctct gatctttttg tttctatcat tttattggct aatatgggca ggaatagcag
     2341 gtgcattagt acaggcaagt ctatattact ggtttttgtg gaagcaacaa gaaaaaggat
     2401 tggattggga gaaaatggtg catgtagaag aacataggtt gcatcagatc tatcaattta
     2461 tcaatttatt cacagatgtt cctgaaatta cggcaaagat caaaagaaga cgctatttgg
     2521 atcctatatt gactcatatt aaaagagaat caaaaaacac ttatctttac ttgtttgcaa
     2581 gaagattggc aagaggttct gattttagtg gtctttattt ccgattagta attataggtg
     2641 ggttattgat tgccggagta gtcgactttt attttagtct gattatcggt ttcttatttt
     2701 tatatttgat cggtttccaa ctgttacctt tagcgaacca gttccgctat atggttctcg
     2761 ttcagcttta tccaatccat aagaatcaga agaagcaggc gattcagtat ctactcacat
     2821 gggtattaat tatcgcgtcg gttatttttg gtttgattgc tgcagtcgta ttagatgggc
     2881 agcagcgatg ggttccgcta gcggcttatt tgattgttac tatgctcttt gttaaactat
     2941 atgtaccaac tcgtttgaag aagatgtcag actgaaaaca aagctgtgta gggtaaaaat
     3001 gagaatgaaa aatagagcgt tgacagtaag aatgctgttc gttctatttt ttttgagaaa
     3061 agctggtttt ttgaatggca attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg
     3121 ttcaggcgac tagaataata agtactgcaa aacaaagagt aagaggacgg gagaatgcat
     3181 gatggagttt cagttagacc aagagtggcg gttacgtccc atcaaaggcg acacaggaaa
     3241 aacatacata ggcatgcgtg atgaggacaa agtctttatt aaacgaaaca cgactccgat
     3301 gttggctgct ctttcaaggg aaggaattac accaaaactt gtttggacaa aacgaacggg
     3361 gaatggcgat actttgacag cacaagagtg gctggaaggt cgcttgctga ctcctaatga
     3421 gattggtcaa agaaacgacg tgattgatgt tttgtaccat ttacatcatt caaattctct
     3481 taaaaccatg ctgacccgta tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa
     3541 atatgaaaaa gaattaccgg aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg
     3601 ttttcttcgt gagcagatcc cgtcaataga taaggaaaac ttatcagtag tacacggaga
     3661 tgtaaaccat cgtaattggt tagtatgtaa aaattattta tatctagtcg attgggattc
     3721 aatcatgttt gcagaccctg cggtggatat tgcaacgatt ttaggaaatt atgtgccact
     3781 gtctagctgg aatcagtggc tgatcagtta tggcatacgc ccgactgacg aaatgcttga
     3841 aaaatttcat tggtatgcgg ccatgaattt gctccaagaa atcaccagat attgcaaacg
     3901 tgaagatcat cgacggatga acgaagaaat tttacaatta aaacgaattt tcagtggctg
     3961 aggtttggcc caaactctgc ggagatttgg ccagaccttt ttaattagag gaaaggaatg
     4021 gagataaata atgcgtgtcc gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc
     4081 gaattttgta attagtgatc caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa
     4141 cgatcatcca atccacattg agattggtat ggggaaaggc cagttcatca ctggaatggc
     4201 aaaagcccat cctgaaatca attatattgg agtagaaatg caggtcagtg ttgtttcgat
     4261 tgcactagac aagttgatcg aacaaccgct tcctaacctg aaacttctac atgtggatgg
     4321 atcagcgttg acagagtatt tcgctgacag cgaagtagat caaatctatc tgaatttttc
     4381 cgatccatgg ccaaaaaaac gtcatgaaaa acggcgattg acatataaga cttttcttgc
     4441 tgtagatgaa cagattcttc gtccaaatgg agaaatccat ttcaaaacag ataatcaagg
     4501 attgtttgag tattctctgg caagtttttc tcaatatggc atgatcctca aacaggtatg
     4561 gttagattta catcaaagtc aatttgaagg caatattatg acggagtatg aagaaaaatt
     4621 ttcttcaaaa ggtcaacgta tttatcgagt agaagctcaa tttcaagata agaccaaaaa
     4681 gaaaagtctg tgaaactaac tttctgcttt cataaaaaca ttaatatcca taaataaatc
     4741 tcagaaaatg aacattacag atgtaatgta ttttctggga tttatttgat ttaaaatgta
     4801 tttatttcat cggtttttcc acaaggtgga atcaattgga agcgagaaaa tgaaaataat
     4861 cctttgtcgg tgatcttcaa ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa
     4921 aaaggatcaa aggttttagc tgctcctagt tgatgtgctt tagttattaa ttcattcaac
     4981 tgttcataga cgatcgaaaa aggttcctgt gtcatgattc cgcctatagg aagagaaaca
     5041 caagctattt cctgctgttt gttgacaacg gtcatcccgc cacctttttg aatcaaaaga
     5101 ttagcagcat acagcatatc ctcgtcgttt gtcccaacga caactagatt atgcgagtca
     5161 tgggcaatcg ttgtcgctat cgctccttgt ttcagctgaa atcctttcac tatgcctaat
     5221 ccaatgtttc ccgtttgatg gtgacgctcg atcaccgcaa tctttaattg gtctttttcg
     5281 attgaaggaa tgaatcgttc gccttgtcgg tccacttttt ctaccaaatc tttagtcact
     5341 agactattcg ggataatttc gatcacatgg gcttgtgaag aagaaagaag cagctctaac
     5401 gaatgtggat ttaatggttg agcgtttagt tttggcagac aacacgtata tgaatttgaa
     5461 tgatcagcaa ataaatggtc ttggagttgc ccagattgaa caactaatgt tcccttatag
     5521 aaaacttcat gaattggcag cttggtaaga tcgtctgtta agaagaaatc agcttgatac
     5581 ccaggggcaa tcgcacctag tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc
     5641 atctgaatgg cttgtaatgg atcgattcca agtgcaatag cttgacggac gatatgatcg
     5701 attgatcctt cttttactaa atcattgatc agtttatcat cggtgacaaa gaagcatctt
     5761 cttgaatttt caggtgtgat cgccggtagt agcgatgcca gatcttttgc aacggtccct
     5821 tctcgaacca ttaaatacat accaagatcc aaacgttctt tagcttcttt tgcagttgta
     5881 gcttcatgat ccgtccgaat acctgctgca ggataaacat ttaactcttc catgccgata
     5941 ccagctgcat gcccgtcaat ttttttctta tgctggtgca tcaattggat cttttctagg
     6001 atgtctgttt cattatttgc tactgcttga tagttcatga cttcagctaa gcctaaaact
     6061 tcgggttttt gtaaaaaagg ggctaatttt tttgcattta aatttgcacc attggtctca
     6121 aaaggggtta caggtacaca agaaggtagc ataaaaaaga tattcatgag tgtttgtctt
     6181 gcatcttcta tcataaactc aagacctgta ctcccagcaa tgtttcctat ttcatgggga
     6241 tctgtcacta cagtagtaac tccatgaagc aaggatactt ttgaaaattc gcttggtgat
     6301 aaaagtgagc tttcgatatg cacatggcca tcgatgaatc ctggcactag atattgccca
     6361 ttagcattat gtacgctttt tccttcgtat tcaccgattc ctacgatcat tccttcgcag
     6421 attgctacat ctttttccat gatttctttc gtaaagacgt tgacgatctt agcatgcttg
     6481 atcactaggt cagcaggctt ttttttactt gctacttcga tatatttcct taatgttgtt
     6541 tccaatggag tgcctcccaa gataaatttt tataagtata acataaattc ggcttttgcc
     6601 aatatttttt tgtaaattca ttttatttga aacaaaaaga tcaaaaagac gtatgttttt
     6661 tgtggagggg agaataatgg aaataaaacg attatcggat caaatgataa gtgaaagttt
     6721 tgccaaacat gcccaagacg tatatatatt gccagcttca gaagtatttt acatctattt
     6781 tcgctatgga aaaaaacgaa cattctggaa gaaagtctct tcggcagatg gacagcaact
     6841 gatctcaaga tttaagtatt tgggacagat ggatatcgga gaaaaaagaa aagctcagtt
     6901 aggtgcattt acttattctt tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg
     6961 agattacctt caacgagaga gtttagtgat tcggctatta tacggtactt ttgaaacacc
     7021 ccatcgttgc tttagttctc gagattttgc aactatttta cagaaaaccg tcagacgtgg
     7081 attatacctt ttcagcggga cagtcggatc aggcaagact tctctaatgt atcggttggc
     7141 tttggaagaa aaattgcaag tgattactat cgaagatcct gtagagatag aagaaccgtt
     7201 ttttctgcag ctacagatca atccgaaaat agcacagact tatgatacac ttttgaaact
     7261 cgcattacgg catcgtccag acttgctaat cattggcgag ataagagatg aatcaactgc
     7321 acatgctgcg gtaagagcag cactaacagg gcaccgggtt tttgcaaccg tacatgcacg
     7381 caatcttgaa ggtacattgg tcagaatcca tgaactgaca aagaaaaaag aagagctatc
     7441 tgaatgttta gctggtgtca tttatcaaga acttttggaa gatgccaatc accatccttc
     7501 tgttttatgg agttatcaat ttatggagga agaatcagta aaaaaatcat gggaggaaag
     7561 ttatgaagaa gctcaggaaa atatttggca gtcagaacta ttctaaagga ttaacaagaa
     7621 gccagcaaaa ccagtttgca caaaccatgg gagaactgct tttatctggt tttagtctgc
     7681 aagaatccct ttatttatta ctcacgatcc gttcgtttcc acctgttatt ttggcaaata
     7741 tccaacatca gctaaaggaa gggcaagcgt ttagtgaggt actttcgcaa ataggatatg
     7801 gagcagagca actggtacag atcgagttag ctgaacgtca tgggaatctg ccgacaactt
     7861 tgttggaaat tgccaaacag tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc
     7921 tgcttgcata tccgatgcta ttgctcacct ttttgctagg tatcttgttt agtatgcggt
     7981 tgttcgtttt gcctcagctt attggttcag gaatggtcat gcaagagcat tggggtattc
     8041 agctgataca agtctttcat tggtatgttc tactattcat cctagcggtt actctattgg
     8101 ctttactagt aaggtatcgt ctgatgaagt tgtctcctgt tgatcagagt gaatggataa
     8161 gcagacaatt attgattgga ccgatatatt ccttgtatca atcggcttat ttgtcactag
     8221 agattggaaa gctttttcat gaaggtttcg aattaaggca aatcatagtc tgcttaaagg
     8281 aaacgaaaca agaaagtctg attcagcatc ttgcctctaa gttgattcaa ggattggagg
     8341 caggtatgtc attggctgag cagttccaga attatcgttt tctatcagaa gaattcagta
     8401 aaattgtttt acagggagag gcaaagggaa gtttaggtaa agaactgatt ttttacagcg
     8461 aatggacaag aaaacagctt ttcaaacgtc ttcatcactg gatgcatttt attcagccaa
     8521 tcattttttt gggcgtagcg gctttgattt tattagttta tgccgctgtt ttattacctg
     8581 tttacggaag catagaggag gttttgccat gaaaaagaga aaaatatctt attatagcgg
     8641 ttttacattg atcgaaatgc taatcgtctt actgatcatc agtgtattag tgttgttgtt
     8701 cgtccctaat ttgtcgcgtt ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat
     8761 ccagttggta gacacacaaa aagagctcta tgcgcttcaa aataatggaa gagttcctac
     8821 tgtggaggaa ttgttgaatg aaggctatat caaacgagaa catgcagaaa tctatcagcg
     8881 gccttgaagg taaggtttta gcatcaggat atacgttgtt tgaatcactc ttactgattt
     8941 tattacttac aacgatcctt tcttttccag ttctcgcatt ttctgcatgg aagaatgaat
     9001 tggcagtcta tcaatttttc ggccaatttg aaaaacgaat ctattctacg caaaaaatcg
     9061 ctattgtgaa tcagatccaa acaggctttt attggaatag tgaagagaat caaatcattt
     9121 ttcatgttcc gaatccagat aaaataaatt gggaagtatt agatattcct gatgagatta
     9181 ccttgaaacg ccatgcttct attacttttg ctgctagaac cgggaatgaa agttcactaa
     9241 aagcttatca attttattgg gaagaaaaag agcggacgat tacgtatcaa ttccagatag
     9301 ggagtggtcg ctatacaaaa agaattgaat aaaacaggat ttgttttgtt ggagtccctt
     9361 acagcgctta tgatcgcttc tgtctgtgtc tacttagtga gctttggcca gctgtttttg
     9421 cttcaggcag aaaagaaaag tggggaggaa ctgctattta ttcgaatgat ttatgaagat
     9481 atcaagacgc agcggatgta tcacaaaaga ccagagcgaa gggaagaaat gatcccatac
     9541 gatatccaaa ttgtcgataa taatggagta atcgaaaaag tgtttgtcaa tatggagaaa
     9601 gaagcgatca gtattgaaaa aacaagtaat tccagcattt acgctgattg aatgtctaat
     9661 tggactgatg atattgagca gtctattgct cacattcaat cttttgattc agcaaaccat
     9721 ccaagtaagt gaatttttgc agcgtaaaga tcaaaaagag tggctaatat ttttagcaca
     9781 attagaagaa gaactcaaga gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta
     9841 cacgatcgag gataaacagt atatcatcga aagatatcag gcaatgatac gtaaaagaag
     9901 aacttccggc ggacatcaac ctatgttaac aagtatcact gagcttcaat tattggaaaa
     9961 agaacacaca atttcttttt atgtacactt tgaaaatgga gaagatggat atgccatatg
    10021 gacaaaaaac gatcagtaga aaatgttata agggctctat tttattttct gtcttgcttt
    10081 tatttctttt atgtacgttt ttattgatgg cagcattaga aagctaccgt ttgtcaatgg
    10141 atcttaatat acgaatcaaa aattattata tagcaaaaat aatgaagcag atggctttat
    10201 cggaacagtc tacgcttgcc gaaagttcgg aaggagtttt ttactacaca actggatctg
    10261 taacatatca gtgggtacaa cagtccctat tcttagaggt ggaagtctca ccgtttatct
    10321 ttcggttcac agaagaagta aaaaatgata cagatactag tacagaatga tggaatgttc
    10381 gtgaaaacgc cctcttttct gttataacac tgtttttaat aaaaaaatgt ttgccgtttc
    10441 acaaataaaa aacagtgaaa acgattgaaa ttttgtcaat tgttcgatag aattaaacag
    10501 gtgaaaaaat tgttataata tatctagaaa tgaggtgcac catgttacca gaaaaaatgg
    10561 aacaagggtt taatcaaaac ctggaagcta ttcagctttt acagaatgca ttgggaactt
    10621 cctttttaga agcctatgtc gaaaatgcag aaaatttaat agatgattat caagtacgtg
    10681 tggtggatgg agtgccgaca aaagagacaa cgcaacggat cacagctctt tatgaagagt
    10741 tgaagaaact ttctttcgag ccagaagaat ggcgcagact gtctcaatta cttttattga
    10801 agggcagcca gacagaacat ttgcaggcga accatcaact cactcctgat agtatcggtt
    10861 ttttatttgt ttttcttatt gaacaactat acacaaataa aaaagcaccg gttaagattc
    10921 tggatattgc ggcaggaatg ggcaacttgt tattaacagt tcttttaaac ctatcgaatg
    10981 cggggtacca aacagaagga attggtgtgg atattgatga tacattgttg gctgtcgcag
    11041 cttctacaag tgatttgaca caagcaaatg ttcaatactt tcatcaagac gggttgcagg
    11101 aattattaat agatcctgtc gattttgcaa tcagcgattt gccaatcggt tattatccga
    11161 atgacgaaaa agcaaaagag tttttaacaa gtaccgaaga aggccatagt tatgcacatc
    11221 atttattgct agagcaatca atgaaatatg tgaagccgga tggttttggt ttgtttttga
    11281 tgccgagtgg atttttagaa acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag
    11341 aaggttatct tcaaggaatg attcaattgc cagatgaatt gttccgtaat aaacaatcgc
    11401 aaaaaagtat tttgatctta cagaaaaaag gaccacaagc acaacaagta aaagaagtct
    11461 tgcttgtgaa acttgcttca ttaaaagaac cagagaaggt gacggaattt ttcaacgagt
    11521 ttaaaaactg gaaatcatct gctctgtaat cattcaacga gaaggagagt catatgtcta
    11581 aaacaattgc aatcaatgct gggagttcaa gtttgaaatg gcaattatat caaatgccag
    11641 aagaaaccgt agttgcgaaa gggatcgtcg aacgtatcgg tttacaagat tctattttta
    11701 ccatcaagta cggcgataat caaaaattcg aacaaatcat ggatatcgaa aaccacgaag
    11761 tagcagtaaa aatgctgtta gatcagttga ttgaactgca aattttaagc tcttatgatg
    11821 aaatcaccgg agtagggcac cgtgttgttc acggaggaga aacttatggg gattcagtag
    11881 tgatcgatga gaaagtaatg gaccgcattg ctgaattagc agaatttgct cctttgcata
    11941 atcctgcaaa cttaatgggg atcaaagcgt ttaaaaagat tttacctgac attttgagtg
    12001 tggctgtatt cgatacatct ttccactcaa caatgccaaa gcaaaattat ttatatagct
    12061 taccggtgtc ttattatgaa gatttcaaag taagaaaata tggcttccat ggcacaagtc
    12121 atcgttatgt gtcggaacgt gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa
    12181 tcatcacttg ccacttagga aatggtgtat cgatcacagc agtcgatgga ggtaaatcca
    12241 tggatacttc tatgggattc acaccattag ctggggtgac aatgggtaca cgttcaggtg
    12301 atatcgatcc agctgttctc ccatatttaa tggaaaaatt agagattgat attgatgaaa
    12361 tgatcaatgt tttgaacaag aaatctggtc tgctaggcct aacaggattg tctagtgata
    12421 tgcgtgatct tgaaaagaac tatgaaaaag aacatattcg tttagcttat gacattttcg
    12481 tggaccgcat ccgaaaatac atcggcggtt atgtgacaac gatgaatggt gttgatgcaa
    12541 ttgtctttac tgctggtatc ggcgagaatg acggacatgt acgtagtgaa atcatcaaag
    12601 gtatgtcttg gtttggttgt gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag
    12661 tagatatctc tacaaaagaa tcaaaagtaa gagtattagt gatcccaaca gatgaggaac
    12721 taatgattgc ccgtgatgtg gagcgtctgc gcggctaagc caaagcattc gttaggataa
    12781 aagatgaaac gcatgcaaga gggaattctt gcatgtgttt tttttgcgga gaagaccgca
    12841 aaatggtaat ttaacttcaa tttcccttct tttttacagg atcttcttag ggaattttgc
    12901 ttggaaaagt ggtcaaaagg gaaaagactg gcattttatt tattgatttt caggaaaatc
    12961 ccaatatttt attttcattc atctttataa gggacattac atatgtaaaa agaaaaaaca
    13021 tttttagatc agcctcgtgg gctgtcgact ttattcttcg ctttatgttt agctagaaaa
    13081 ccgatccgaa acattcattg atcgaataat aaaaacttga ttttccacct gtactttgcc
    13141 tgaatcgtga aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc
    13201 aatcagttgg ccgagcggct ggcggctgat ctgctcctga tcaataacga gtgcttcttg
    13261 gaagtgttgc tgcaaaaagt agcggtcatg gctgactgct aggagcgttc cttgatagtc
    13321 ttgcagcatt tcttcaattt cttctcgagc atagctgtcg aggtgattgg ttggttcatc
    13381 caaaatcagg acgttgattt tttctaaaaa gagtttcagc aacgccaaac ggattttttc
    13441 accaccggat aaatctttga tccgtttacc cacatcgctt tgataaaagc cacttttggc
    13501 taattgccgt cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg
    13561 gctatcggga tcggcaaact gcagctgctg cggcaaataa ccgatttgca agctactacc
    13621 taatttgatg gtgccaccgt aaggcttttc ctcaccgagt aataatttga gtaaggttga
    13681 ttttcctgca ccattgtcgc cgatgatggc taaatgatct ccacgataga ctgcaaaact
    13741 gctgtctttg aatagtactt gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac
    13801 ttctttgcca gattttccac cattagacaa gctttgcagc cgatttttcg ggggaggtgg
    13861 cggtttgacg agtgtcagtt tcgccaggcg gcgttccagt tctttggctt ttttgaagaa
    13921 ggactcattg tctccttcat gtgcccactg acgaaaacga cgaatcatga ctttcaaccg
    13981 ctggacttct ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt
    14041 caagactcga aaacggctgt agtttccggg atactcaatc aatgcacctt cttccagttc
    14101 ccatgttttt gtggtgactt gatcgagaaa ttgccgatca tgagaaacga ccagaaccga
    14161 tttttttgta ttctgtaaat agttttccaa ccagcgaata ctgttaagat ctaaatgatt
    14221 ggtcggttca tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg ctaaagcgac
    14281 tttaactcgt tcgcctccgc taagttctgt taaccgtgct tcctgacagt gtggaatccc
    14341 taagccttgc aagatcgctg caatccggtc ttctaatacg taaccgccta gttcttcaaa
    14401 gcgatgctgc agctcaccgt aacgggccag acacttctct aagtttgtat ctggttctgt
    14461 catcaatgct tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag cggcagaatg
    14521 gagcaagtag tggttgacgg tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat
    14581 ctttaagcct tttttgcgac tgataacacc gctgtcgaca ccttctctgc cggtcaagat
    14641 ttgtaaaaag gttgatttgc cagttccgtt gatatcgact aaggcaattt tttcaccagc
    14701 ttcaatttga caggataaat cctcaaataa tggattcccg ccgattgttt ttttaataga
    14761 aattgcttgt atgatcatga tctctctcct tgagaccact caaaaaggcc atggttatcc
    14821 ctggcctgtg ttatttaaac tcagattatc atgagaaaaa taccttgaga tggccattga
    14881 ttttacgtga attcaactgt tttggacaaa cttgtcccgt caacagcaac tgtaaaacca
    14941 attgcatggc taaatgcgat atggtatcgt tggtaatttt cattacttgt tattttagtc
    15001 atgcgcatca tagtattttc cctccttcgt gtagttgtct tttccagtgt acctgaaagt
    15061 gtcagaaagt tcaactagcg ctgacattta cttaatattt atcgatgtgt aattctgtca
    15121 gagagctttt tttatctgac tgagggtaat gtctcgtgga ccacatcatt gtattctgag
    15181 ctaaaccagc gttcaggcga aatcatatgg tgtttgataa aaaactttgt ctacatgact
    15241 tccttttgcc atttgcttaa taccgttgca ttaaacagct cttctctttg gaaatctcac
    15301 caacatcgcg ttcaggagta tcgttgtggt tgcaatggca agcggtagcc tttcatcaat
    15361 tcttgaatga tttaccgaat gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa
    15421 attttagttt ggcgataaaa tcggtatccg ccatcgatac ttcatatttt ttcgtatcag
    15481 attcatcgga aacttccaca ttgacgaacc aataagcttt ggcatgctta ggtcgtttta
    15541 aaccacacca gggcgactaa tcgccctggt gttcactttt tttattttac ttgaactcat
    15601 tgactgtttt ccctttattt taaacattat aaagaccgat gagtgcttgc cgcgtctttt
    15661 gtttttatca cggttttttg tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg
    15721 ttaagctttg gtgtggtctt tagctggcaa ttggttttct aaatcagttc ggacaacaag
    15781 cacatcgcaa gctgcgtttc taataacata ttcagaaaca gaaccaataa ataaacgttc
    15841 tacagcattc aatcctgtgg ctcctaacat aatcaaatct acttgattat cttctgggat
    15901 ttctctggca ataatttgtt ttggtgagcc atattctacg acagaggtta cattattcaa
    15961 gccagctttc ttcgcattgc tttcataatc tgccaatgtt tgctttgcca tttcagtagc
    16021 ttgttctgca agcattccat caaaggatga aacggtttgg aaagaacgtg tgtcaatgac
    16081 atgtgctaat agaagttctc cgttatttcg gatagctaca ttgaccgcct ttttaaaagc
    16141 taattcagct tcatcagaac catctacggc aaccatgatt tttttgtatt gttgtaacat
    16201 aagcattcac tcctcaagtc aatcgtattg tactctttta ttgtagaaca ctttagggaa
    16261 aaagagaatt atttactgct ttggttcagc aatttttttt gcaaatcgga tactgaaggt
    16321 ggcagaaaca ataattacga tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa
    16381 agctgttaaa atacccatta cgcctaaaac agcatatgca gcaccgtaaa attgtaggaa
    16441 tccttgattt ttatcatttt tgttgatcaa tacaaaaaag atatcctgtt tattaaataa
    16501 ataatagcta acgaataata atactaaagt ggcgataatc attaaaatcc taatcatgaa
    16561 caaatcctcc aaaataagca acaaatagaa aaagcaattt gccaagagca aattgcttta
    16621 ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc
    16681 gaattcaagc aggtgggtcc catggtcgta gcttcgaact accaaatgat aaggcatgtt
    16741 accagcagtg acacaagacg ggtcccaaga tatcaataaa aatgttcggt caacacacaa
    16801 agaggacaat tcgaacatca cagatttccc acctctatag tagcatatga atttaaaaag
    16861 agcaatgaga gagctcgcag aaacagaacc gttttcttgt ctagtttttt ggaggatgct
    16921 ttttccactc ttttatacgg taatactgtt gacccagagc ctgttcatat ttccctgtat
    16981 cttttggctg ataatactga gcattcttca gtttatctgg taaatattgc tgattgaccc
    17041 aagcctgatc aaaatgatga ggatactgat aacccacgcc acggttgagt gatttcgcac
    17101 ctttgtaatg gctatctctc aaatgatcag gaacatctcc agctttgccc tctcgaatat
    17161 cagcaagtgc tgcatctaat gccatgtagg cagaattgga ttttggcgac aagcaaagat
    17221 cgacgacgac gtctgctagt gggatacgtg cctcaggcaa tcccaatttt tcagcagcta
    17281 atacagcgtt cactgtacga gccgcagcag caggattacc taaacctata tcttcataac
    17341 cgataaccat cagacgccgg caaatacttg ccagatcacc tgcctcaact aaacgggcga
    17401 gataatgaag tgcagcatcc acatcacttc cacgaatcga tttttgaaaa gccgaaataa
    17461 cgtcataatg agcatctcca tttttgtcat gggtaagagc ttttctttgg acacattctt
    17521 caatgatcga cagtgtaaga tgaatccttc cttctttatc tgaaggagtc gatagtgtag
    17581 ccagttccag cccattcaat gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa
    17641 gaagagcatc ttcgtctaac tggattgctt gttgtccaag gccgcgttct ttatccctta
    17701 aagcatgttc gactgccaac tggatgtctt gttctgttaa tggtttgact tcaaagatct
    17761 gtgtacggct gcggatcgct ggattgatgg taatataagg attctcagta gtcgctccga
    17821 tcaagatgat tcggccactt tctaaatgag gaagcagaaa atcctgtttt gttttatcta
    17881 atcggtgtac ttcatccaat aagaggatca ctgtaccact catttttgct tcttccgcta
    17941 caacttgtag atcctttttt gaatctgtag cggcgtttag catgcgaaat gcataattag
    18001 tagagccggc gatcgcactt gcgatactcg tttttccagt accaggaggt ccatatagaa
    18061 tcatggaaga gagcatgcga gcatcgacca tgcggcgtat gatttttcct tccccgacta
    18121 gatgctgctg accaacgact tcatcgatcg ttcgaggtct catgcgataa gccaatggct
    18181 gttgcatagg attttcctcc ttcttgtttt tacgtccctt aattatatca tatttagcaa
    18241 gatgcctaga aagaacagcc ttctgttgtt ttgttttcat agcttatcca actaatattg
    18301 tgtatgataa agtaggtgag aaaaatgacg gataaacaaa agatagatga atttttttgg
    18361 aataaaacga cgccagaagt ggcacgggat ttattgggga tgtatttgga gcatgacaca
    18421 ccagaaggaa gattagctgg atatattgta gacgcggaag catacctagg tccagaagac
    18481 gaggccgccc atagctatgg tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa
    18541 ccaggtacga tctaccttta cacgatgcat actcatcgga ttttgaacat cattactcag
    18601 ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg aacccgccgc aatgattgat
    18661 caaatgagta aaaatcgtgg cggaaaaact ggtccagata tcagcaacgg tccgggaaaa
    18721 ttagtggaag cattagcaat tcctcaagaa ctttatggtc agtctatagc agatagttcg
    18781 ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc
    18841 ggtatcccaa acaaaggtgt atggacagaa aagccgttgc gttttgttgt gtcgggaaat
    18901 ccttatatct ctttacaaag aaaaaaccaa atcgagaaag attggggttg gaggaaagaa
    18961 aatgaaaaag aaggatctat taacatattt agatgaaata attgaaaaac aagtgacaga
    19021 ctatgatgta gctttagatt gggatacaaa aaatcatacg atcgagatcg ttgttcgctt
    19081 gtttgcagaa aataaagcac aatttgagat tgatgatgca gaagggatcg tttcgcaaga
    19141 accaattatc gaatttgaag acggcgtact tctttttaat cctcagaaat ctgtttttga
    19201 tgaacgcaat tatctagcag taatccctta tgaaggaaaa aaagggttgg caaaatcagt
    19261 agctgattct ttagtggaat atctaaatga agtattagct caagggcaaa gcgatttgct
    19321 tgattttttg gatgaagacg atgacgaagc tgtattcgag cttcactggg ataatcaaca
    19381 actagcagaa ttagtagaag taaaacagca aaatgaagca gatacttatt taccgtaccc
    19441 aagttattaa ggagtgacag atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga
    19501 agcagtcgaa gcagttgcta atattttgat ggaggcaggg gcgagtggtg tcgcgattga
    19561 agattcttta gatgttgaac attttcagtc ggatcaatat ggtgagattt tagataaaaa
    19621 tgattttaca tcgctgaaag aaggcgcact agtcatggct tattttccgg aaacgacttt
    19681 cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca caattacctg agtttggtct
    19741 agctattgga aaaaataaag taacagttag tgaagtagag gaaagcaact gggcaacagc
    19801 ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg
    19861 ggaaaactac cagacagaag atcctctaga aaaaattatc acactagatc ctggtatggc
    19921 atttggcaca ggcactcatc ccacgacttc cttgacattg acagcactag aaacagtctt
    19981 gcgtggtggt gaaacattgc tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc
    20041 aaaatatctt ggtgcaaaag atgtctatgc ctttgattta gacgaagtag ccgttcgttc
    20101 ggcgaaagaa aatatggata tgaatgaaat agccaaagat gtccatgtat cagctaacga
    20161 tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat
    20221 catcttattg atgatcccag atgcttggcg tttactgaag caaacaggca cgctgatcgt
    20281 ttcaggaatc attgaagaga agaagcaaat ggttctggat gccatgttag aacaagggtt
    20341 tatcgtggat caaattttcc agcaaaaaga ctggtatgca attattttga aaaaaccaga
    20401 ggtggaatag atgcagcggt attttttaaa agaatcatat gaatcaaaag actattttgt
    20461 tttagaaggg gaaaactatc accacattgt acgggttatg aggatggaac ccgaggatga
    20521 agtttttttg gcattcaatg atcaaatagc cattgttgca gaaatcacgg agattactga
    20581 agaggctgtt tatctgaaag aaattaaaaa agaagaaaat caaaaggaac tgccaagttc
    20641 gattaccata gcgagtggct atcctaaagg ggaaaagctg gattggatcg ttcagaaatc
    20701 cactgaatta ggagcgcaag cttttcttgg tttcccggca tcagcctctg tcgttaaatg
    20761 ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa aagattgctc aagaggcagc
    20821 ggaacaatcc catcggcaga tcgtgccatc agtagagtta ttggaaacga aacaagattt
    20881 atttgagcgg ctagatgatt atgacgtggt cttggttgct tatgaggaat cagcaaagca
    20941 aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca aaaccagata gtcgaatctt
    21001 agctgtcttt ggaccagaag gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca
    21061 caaagggata ctttgtggat taggaccaag aatccttcgt acagaaacag cacctcttta
    21121 tttattaagt gctgctagct atcaatggga attaatgaat ccttctttca gctaacgttc
    21181 ttcattaata aaagagtatc gacagaaaga attgaaaaaa tagtcaacaa tttgtataat
    21241 agaaacaatt atttatacaa tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga
    21301 acatttccgt aaaaaaacgt ataactgcac ggattttggg acaattatca aacagatatt
    21361 tgtcccgatt cccgttttac cgaaaaaaga aagggggatc gattcctgtg acaaaagaag
    21421 aaattttaac gggacccggt gtcataaagc tcgttagtca atacatgggt cccgaacatg
    21481 tagcatttgt tgagaaagca tgcgaatacg cgactgctgc acatgacgga cagtttagaa
    21541 aatcaggcga accctacatt attcatccta tccaagtcgc aggtatatta gcagatttaa
    21601 aaatggatcc ccatacagtg gctacaggct tcttacatga tgttgttgaa gatacagaaa
    21661 tcactttaga agatctgaga gaagaatttg gcgatgacgt tgctatgtta gtggacggcg
    21721 taaccaaatt agggaaaata aaatataaat cccacgaaga gcagctagca gaaaaccacc
    21781 gaaagatgct gctagcaatg gctcaagatt tacgagttat catggtcaaa ttagctgata
    21841 gactacataa catgcgtacg ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc
    21901 aggaaacttt agaaatctat gcgcctcttg cacatcgtct agggatcagc cggatcaaat
    21961 gggaattaga agatacagca cttcgttatc taaatccaaa acagtattac cgtatcgtcc
    22021 acttgatgca gacgaagaga gaagaacgtg aaaaatacgt aagtggtact gttgaagata
    22081 ttcgaatagc gacagaagag ctggggattt ttgcagaaat ctatggacgg ccaaaacaca
    22141 tttattcaat ctatcgtaaa atgaaagatc agaaaaagca gttcaacgaa atttatgact
    22201 tgctagctat tcgagtgatc gtagattcaa tcaaggattg ttatgctgta ttaggagcaa
    22261 tccacacaaa atggaaacca atgcccggca gatttaagga ttatattgcc atgccaaaag
    22321 ctaatatgta ccaatctttg catactaccg tgatcggtcc ggcaggaaat ccggtagaaa
    22381 ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt cggggttgct gcacactggg
    22441 cctataaaga aggaaaaaat gaaaaagtag aaccagatgg tatgacgaaa caattaagct
    22501 ggttccatga gatactcgaa cttcaagacg aaagctatga tgcttctgaa tttatggaag
    22561 gcgtaaaagg agatatcttt agtgataaag tctacgtctt cacaccaaaa ggagacgtta
    22621 ctgagttacc aaaaggatcc ggaccattgg actttgcata cagtatccat acagatatcg
    22681 gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt gcagcttgat tacaaattga
    22741 aaaacggaga tatcattgag atcatgactt ctccaaattc atttggccca agtcgcgact
    22801 ggttgaaatt agttgctact agcaaagcaa gaaataagat caaacgtttc ttcaaagccc
    22861 aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt ggtcaaatgt attacagatc
    22921 taggatttac gcctaaagat attttgacga agaacaaact gcaagaagca ctcgatcgtt
    22981 ttaattatca aacagaagat gatctctatg cagctgtagg gtatggagaa gttagcccct
    23041 tgacgatggc caatcgtctg actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa
    23101 agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac
    23161 gtcatgaagg tggcgttgtc attcaagggg tagaaaactt actaattcgt atcagtcgct
    23221 gctgtaatcc gattcctggt gatgatatcg ttggttatat cactaaaggc agagggatat
    23281 ccattcatcg tcgagattgt ccgaatgttc agcctgacaa accaaatgta gcagaacgtt
    23341 tgattgaagt cgaatgggaa gatacatcga atacacgaaa agagtatgat gcagatttgg
    23401 aaatttacgg ctataatcgt tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc
    23461 taacgaaaaa tctcaacagc gttgaagcac ggacgaataa agataaaatg gcgacgatcc
    23521 atttgacggt tggtatccag aatttatccc acctaaagag tatcgtggat aaaatcaaag
    23581 cagtacctga tgtctacagt gtacgccgga cgaatggata ggaggacaga tatgagagca
    23641 gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt
    23701 ggctacggcc tacttgtttt attaggcatt catgaaaccg atacgcaaaa agatgtggac
    23761 tatctggtaa aaaaaattgc acaaatgcgt atttttgaag atgaacaagg aaaaatgaat
    23821 attagtgtag aagatgtggg aggacaaatt ttaagtatct ctcaattcac actgtttgcg
    23881 gatacgaaaa aaggaaatcg accaagcttt gtggaagccg cacgaccaga aacagctatt
    23941 cctttatacg aatcattcaa tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga
    24001 caattcggag cagatatgtc ggtttctctc gtcaatgatg gacctgtaac gatcattatt
    24061 gattctaaaa acaaataaag taatctggtt ttcactagta aaaactatga aacacgtcgc
    24121 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
    24181 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
    24241 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
    24301 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
    24361 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
    24421 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
    24481 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
    24541 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
    24601 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
    24661 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
    24721 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
    24781 cgattgatca cataaaaggt gtcgagaatc gcaaaaaatc agaaaaatgg gcgcaaggct
    24841 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
    24901 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
    24961 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
    25021 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
    25081 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
    25141 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
    25201 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
    25261 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
    25321 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
    25381 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
    25441 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
    25501 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
    25561 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
    25621 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
    25681 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
    25741 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
    25801 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
    25861 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
    25921 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
    25981 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
    26041 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
    26101 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
    26161 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
    26221 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
    26281 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
    26341 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
    26401 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
    26461 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
    26521 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
    26581 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
    26641 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
    26701 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
    26761 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
    26821 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
    26881 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
    26941 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
    27001 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
    27061 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
    27121 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
    27181 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
    27241 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
    27301 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
    27361 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
    27421 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
    27481 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
    27541 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
    27601 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
    27661 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
    27721 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
    27781 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
    27841 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
    27901 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
    27961 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
    28021 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
    28081 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
    28141 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
    28201 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
    28261 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
    28321 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
    28381 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
    28441 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
    28501 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
    28561 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
    28621 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
    28681 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
    28741 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
    28801 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
    28861 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
    28921 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
    28981 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
    29041 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
    29101 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
    29161 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
    29221 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
    29281 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
    29341 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
    29401 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
    29461 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
    29521 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
    29581 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
    29641 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaattaggcg
    29701 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
    29761 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
    29821 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
    29881 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
    29941 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
    30001 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
    30061 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
    30121 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
    30181 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
    30241 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
    30301 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
    30361 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
    30421 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
    30481 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
    30541 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
    30601 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
    30661 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
    30721 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
    30781 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
    30841 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
    30901 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
    30961 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
    31021 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
    31081 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
    31141 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
    31201 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
    31261 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
    31321 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
    31381 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
    31441 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
    31501 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
    31561 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
    31621 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
    31681 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
    31741 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
    31801 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
    31861 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
    31921 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
    31981 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
    32041 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
    32101 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
    32161 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
    32221 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
    32281 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
    32341 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
    32401 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
    32461 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
    32521 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
    32581 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
    32641 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
    32701 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
    32761 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gattggacta
    32821 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
    32881 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
    32941 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
    33001 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
    33061 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
    33121 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
    33181 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
    33241 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
    33301 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
    33361 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
    33421 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
    33481 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
    33541 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
    33601 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
    33661 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
    33721 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
    33781 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
    33841 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
    33901 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
    33961 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
    34021 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
    34081 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    34141 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    34201 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    34261 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    34321 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    34381 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    34441 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    34501 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    34561 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    34621 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    34681 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    34741 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    34801 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    34861 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    34921 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    34981 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    35041 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    35101 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    35161 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    35221 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    35281 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    35341 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    35401 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    35461 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    35521 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    35581 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    35641 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    35701 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    35761 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    35821 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    35881 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    35941 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    36001 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    36061 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    36121 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    36181 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    36241 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    36301 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    36361 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    36421 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    36481 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    36541 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    36601 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    36661 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    36721 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    36781 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    36841 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    36901 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    36961 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    37021 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    37081 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    37141 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    37201 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    37261 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    37321 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    37381 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    37441 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    37501 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    37561 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    37621 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    37681 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    37741 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    37801 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    37861 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    37921 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    37981 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    38041 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    38101 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    38161 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gaaagaatag agctggaaga
    38221 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    38281 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    38341 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    38401 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    38461 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    38521 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    38581 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    38641 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    38701 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    38761 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    38821 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    38881 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    38941 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    39001 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    39061 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    39121 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    39181 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    39241 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    39301 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    39361 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    39421 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    39481 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    39541 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    39601 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    39661 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    39721 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    39781 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    39841 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    39901 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    39961 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    40021 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    40081 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    40141 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    40201 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ctgatcaaaa
    40261 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    40321 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    40381 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    40441 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    40501 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    40561 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    40621 cgatttcgtc tatggtgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    40681 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    40741 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    40801 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    40861 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    40921 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    40981 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    41041 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    41101 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    41161 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    41221 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    41281 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    41341 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    41401 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    41461 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    41521 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    41581 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    41641 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    41701 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    41761 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    41821 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    41881 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    41941 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    42001 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    42061 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    42121 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    42181 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    42241 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    42301 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    42361 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    42421 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    42481 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    42541 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    42601 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    42661 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    42721 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    42781 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    42841 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    42901 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    42961 gtctcttctg gaagcatatg aaattctttc tcagccagct gaaaaatctc tttttctggt
    43021 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    43081 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    43141 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    43201 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    43261 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    43321 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    43381 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    43441 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    43501 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    43561 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    43621 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    43681 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    43741 ggaattttcg aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa
    43801 taacgttatt tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat
    43861 actacg
//
LOCUS       DACDID010000006        42635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDID010000006 DACDID010000000
VERSION     DACDID010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.006"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            402..1091
                     /locus_tag="IV236_000259"
     CDS             402..1091
                     /locus_tag="IV236_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8770485.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1078..2286
                     /locus_tag="IV236_000260"
     CDS             1078..2286
                     /locus_tag="IV236_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8770486.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2580..3851
                     /gene="serS"
                     /locus_tag="IV236_000261"
     CDS             2580..3851
                     /gene="serS"
                     /locus_tag="IV236_000261"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8770487.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4400..5884)
                     /gene="guaB"
                     /locus_tag="IV236_000262"
     CDS             complement(4400..5884)
                     /gene="guaB"
                     /locus_tag="IV236_000262"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8770488.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6389..7084)
                     /locus_tag="IV236_000263"
     CDS             complement(6389..7084)
                     /locus_tag="IV236_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8770489.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7174..8274)
                     /gene="ychF"
                     /locus_tag="IV236_000264"
     CDS             complement(7174..8274)
                     /gene="ychF"
                     /locus_tag="IV236_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8770490.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8283..8474)
                     /locus_tag="IV236_000265"
     CDS             complement(8283..8474)
                     /locus_tag="IV236_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8770491.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8495..9385)
                     /locus_tag="IV236_000266"
     CDS             complement(8495..9385)
                     /locus_tag="IV236_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8770492.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9372..10139)
                     /locus_tag="IV236_000267"
     CDS             complement(9372..10139)
                     /locus_tag="IV236_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8770493.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10151..10864)
                     /gene="rsmG"
                     /locus_tag="IV236_000268"
     CDS             complement(10151..10864)
                     /gene="rsmG"
                     /locus_tag="IV236_000268"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8770494.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11096..12997)
                     /gene="mnmG"
                     /locus_tag="IV236_000269"
     CDS             complement(11096..12997)
                     /gene="mnmG"
                     /locus_tag="IV236_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8770495.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13014..14411)
                     /gene="mnmE"
                     /locus_tag="IV236_000270"
     CDS             complement(13014..14411)
                     /gene="mnmE"
                     /locus_tag="IV236_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8770496.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14755..15093
                     /locus_tag="IV236_000271"
     CDS             14755..15093
                     /locus_tag="IV236_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770497.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15261..16019)
                     /locus_tag="IV236_000272"
     CDS             complement(15261..16019)
                     /locus_tag="IV236_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8770498.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16037..16855)
                     /locus_tag="IV236_000273"
     CDS             complement(16037..16855)
                     /locus_tag="IV236_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8770499.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16852..17208)
                     /gene="rnpA"
                     /locus_tag="IV236_000274"
     CDS             complement(16852..17208)
                     /gene="rnpA"
                     /locus_tag="IV236_000274"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8770500.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17326..17460)
                     /gene="rpmH"
                     /locus_tag="IV236_000275"
     CDS             complement(17326..17460)
                     /gene="rpmH"
                     /locus_tag="IV236_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8770501.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17983..19317
                     /gene="dnaA"
                     /locus_tag="IV236_000276"
     CDS             17983..19317
                     /gene="dnaA"
                     /locus_tag="IV236_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8770502.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19515..20645
                     /gene="dnaN"
                     /locus_tag="IV236_000277"
     CDS             19515..20645
                     /gene="dnaN"
                     /locus_tag="IV236_000277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8770503.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20874..21116
                     /gene="yaaA"
                     /locus_tag="IV236_000278"
     CDS             20874..21116
                     /gene="yaaA"
                     /locus_tag="IV236_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8770504.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21103..22227
                     /gene="recF"
                     /locus_tag="IV236_000279"
     CDS             21103..22227
                     /gene="recF"
                     /locus_tag="IV236_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8770505.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22224..24170
                     /gene="gyrB"
                     /locus_tag="IV236_000280"
     CDS             22224..24170
                     /gene="gyrB"
                     /locus_tag="IV236_000280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8770506.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24190..26661
                     /gene="gyrA"
                     /locus_tag="IV236_000281"
     CDS             24190..26661
                     /gene="gyrA"
                     /locus_tag="IV236_000281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8770507.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26897..27196
                     /locus_tag="IV236_000282"
     CDS             26897..27196
                     /locus_tag="IV236_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8770508.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27246..27758
                     /gene="ssb"
                     /locus_tag="IV236_000283"
     CDS             27246..27758
                     /gene="ssb"
                     /locus_tag="IV236_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8770509.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27784..28020
                     /gene="rpsR"
                     /locus_tag="IV236_000284"
     CDS             27784..28020
                     /gene="rpsR"
                     /locus_tag="IV236_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8770510.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28163..30136
                     /locus_tag="IV236_000285"
     CDS             28163..30136
                     /locus_tag="IV236_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8770511.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30142..30594
                     /gene="rplI"
                     /locus_tag="IV236_000286"
     CDS             30142..30594
                     /gene="rplI"
                     /locus_tag="IV236_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8770512.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30868..32235
                     /gene="dnaB"
                     /locus_tag="IV236_000287"
     CDS             30868..32235
                     /gene="dnaB"
                     /locus_tag="IV236_000287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8770513.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33015..34307
                     /locus_tag="IV236_000288"
     CDS             33015..34307
                     /locus_tag="IV236_000288"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8770514.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35043..35906
                     /locus_tag="IV236_000289"
     CDS             35043..35906
                     /locus_tag="IV236_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8770515.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36035..37657)
                     /locus_tag="IV236_000290"
     CDS             complement(36035..37657)
                     /locus_tag="IV236_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770516.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37906..38427
                     /locus_tag="IV236_000291"
     CDS             37906..38427
                     /locus_tag="IV236_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770517.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38613..40343)
                     /gene="spxB"
                     /locus_tag="IV236_000292"
     CDS             complement(38613..40343)
                     /gene="spxB"
                     /locus_tag="IV236_000292"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8770518.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40508..41326
                     /locus_tag="IV236_000293"
     CDS             40508..41326
                     /locus_tag="IV236_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8770519.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41429..41626)
                     /locus_tag="IV236_000294"
     CDS             complement(41429..41626)
                     /locus_tag="IV236_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770520.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41795..42373
                     /locus_tag="IV236_000295"
     CDS             41795..42373
                     /locus_tag="IV236_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8770521.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        12949 a         8197 c         8430 g        13059 t
ORIGIN      
        1 aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtca tttccaacat
       61 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
      121 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      181 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      241 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      301 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      361 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      421 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      481 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      541 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      601 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      661 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      721 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      781 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      841 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      901 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      961 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
     1021 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1081 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1141 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1201 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1261 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1321 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1381 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1441 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1501 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1561 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1621 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1681 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1741 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1801 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1861 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1921 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1981 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     2041 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2101 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2161 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2221 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2281 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2341 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2401 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2461 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2521 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2581 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2641 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2701 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2761 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2821 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2881 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2941 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     3001 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3061 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3121 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3181 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3241 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3301 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3361 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3421 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3481 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3541 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3601 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3661 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3721 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3781 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3841 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3901 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3961 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     4021 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4081 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4141 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4201 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4261 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4321 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4381 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4441 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4501 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4561 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4621 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4681 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4741 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4801 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4861 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4921 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4981 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     5041 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5101 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5161 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5221 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5281 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5341 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5401 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5461 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5521 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5581 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5641 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5701 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5761 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5821 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5881 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5941 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     6001 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6061 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6121 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6181 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6241 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6301 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6361 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6421 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6481 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6541 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6601 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6661 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6721 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6781 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6841 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6901 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6961 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     7021 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7081 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7141 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7201 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7261 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7321 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7381 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7441 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7501 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7561 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7621 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7681 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7741 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7801 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7861 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7921 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7981 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     8041 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8101 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8161 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8221 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8281 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8341 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8401 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8461 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8521 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8581 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8641 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8701 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8761 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8821 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8881 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8941 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     9001 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9061 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9121 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9181 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9241 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9301 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9361 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9421 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9481 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9541 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9601 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9661 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9721 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9781 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9841 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9901 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9961 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
    10021 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10081 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10141 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10201 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10261 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10321 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10381 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10441 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10501 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10561 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10621 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10681 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10741 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10801 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10861 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10921 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10981 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    11041 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11101 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11161 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11221 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11281 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11341 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11401 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11461 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11521 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11581 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11641 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11701 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11761 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11821 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11881 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11941 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    12001 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12061 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12121 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12181 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12241 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12301 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12361 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12421 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12481 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12541 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12601 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12661 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12721 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12781 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12841 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12901 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12961 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    13021 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13081 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13141 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13201 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13261 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13321 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13381 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13441 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13501 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13561 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13621 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13681 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13741 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13801 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13861 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13921 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13981 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    14041 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14101 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14161 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14221 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14281 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14341 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14401 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14461 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14521 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14581 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14641 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14701 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14761 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14821 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14881 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14941 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    15001 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15061 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15121 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15181 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15241 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15301 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15361 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15421 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15481 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15541 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15601 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15661 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15721 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15781 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15841 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15901 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15961 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    16021 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16081 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16141 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16201 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16261 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16321 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16381 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16441 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16501 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16561 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16621 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16681 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16741 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16801 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16861 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16921 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16981 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    17041 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17101 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17161 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17221 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17281 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17341 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17401 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17461 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17521 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17581 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17641 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17701 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17761 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17821 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17881 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17941 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    18001 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18061 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18121 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18181 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18241 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18301 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18361 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18421 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18481 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18541 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18601 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18661 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18721 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18781 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18841 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18901 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18961 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    19021 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19081 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19141 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19201 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19261 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19321 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19381 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19441 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19501 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19561 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19621 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19681 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19741 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19801 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19861 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19921 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19981 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    20041 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20101 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20161 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20221 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20281 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20341 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20401 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20461 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20521 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20581 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20641 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20701 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20761 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    20821 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    20881 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    20941 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    21001 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    21061 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    21121 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    21181 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    21241 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    21301 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    21361 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    21421 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    21481 agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    21541 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    21601 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    21661 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    21721 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    21781 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    21841 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    21901 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    21961 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    22021 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    22081 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    22141 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    22201 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    22261 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    22321 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    22381 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    22441 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    22501 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    22561 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgttaacg
    22621 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    22681 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    22741 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    22801 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    22861 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatta
    22921 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    22981 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    23041 attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga acacatgaat
    23101 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    23161 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    23221 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    23281 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    23341 aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc agagctcgtc
    23401 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    23461 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    23521 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    23581 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    23641 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    23701 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    23761 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    23821 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    23881 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    23941 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    24001 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    24061 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    24121 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    24181 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    24241 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    24301 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    24361 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    24421 taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc acagccattc
    24481 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    24541 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    24601 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    24661 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    24721 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    24781 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    24841 tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt
    24901 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    24961 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    25021 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    25081 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg
    25141 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    25201 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta tatcgaacat
    25261 cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga agcaagggca
    25321 cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    25381 cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc
    25441 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    25501 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    25561 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    25621 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    25681 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    25741 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    25801 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    25861 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    25921 gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc gagtgaaagc
    25981 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    26041 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    26101 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    26161 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    26221 gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct gcgtgaggaa
    26281 gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt gatcacagaa
    26341 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    26401 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    26461 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    26521 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    26581 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    26641 gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa gaatgaccga
    26701 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    26761 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca gagcttgtga
    26821 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    26881 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    26941 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    27001 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    27061 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    27121 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    27181 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    27241 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    27301 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    27361 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    27421 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    27481 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    27541 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    27601 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    27661 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    27721 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    27781 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    27841 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    27901 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    27961 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    28021 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    28081 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    28141 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    28201 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    28261 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    28321 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    28381 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    28441 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    28501 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    28561 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    28621 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    28681 tggtatttta tctattgata actatgcaga tgccattgat aaattggatg acaaagagat
    28741 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    28801 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    28861 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    28921 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    28981 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    29041 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    29101 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    29161 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    29221 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    29281 cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    29341 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    29401 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    29461 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    29521 tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    29581 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29641 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29701 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29761 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    29821 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    29881 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    29941 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    30001 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30061 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30121 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30181 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30241 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30301 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30361 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30421 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30481 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30541 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30601 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30661 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30721 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30781 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    30841 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    30901 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    30961 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    31021 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31081 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31141 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31201 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31261 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31321 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31381 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31441 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31501 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31561 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31621 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31681 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31741 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31801 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    31861 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    31921 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    31981 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    32041 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32101 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32161 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32221 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32281 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32341 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32401 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32461 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32521 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32581 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32641 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32701 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32761 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    32821 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    32881 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    32941 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    33001 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33061 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33121 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33181 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33241 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33301 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33361 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33421 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33481 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33541 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33601 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33661 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33721 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33781 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    33841 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    33901 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    33961 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    34021 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34081 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34141 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34201 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34261 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34321 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34381 acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34441 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34501 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34561 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34621 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34681 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34741 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    34801 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    34861 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    34921 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    34981 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35041 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35101 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35161 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35221 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35281 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35341 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35401 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35461 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35521 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35581 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35641 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35701 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    35761 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    35821 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    35881 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    35941 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36001 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36061 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36121 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36181 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36241 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36301 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36361 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36421 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36481 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36541 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36601 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36661 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36721 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    36781 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    36841 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    36901 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    36961 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37021 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37081 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37141 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37201 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37261 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37321 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37381 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37441 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37501 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37561 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37621 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37681 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37741 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    37801 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    37861 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    37921 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    37981 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38041 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38101 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38161 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38221 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38281 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38341 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38401 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38461 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38521 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38581 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38641 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38701 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    38761 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    38821 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    38881 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    38941 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39001 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39061 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39121 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39181 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39241 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39301 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39361 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39421 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39481 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39541 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39601 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39661 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39721 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    39781 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    39841 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    39901 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    39961 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40021 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40081 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40141 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40201 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40261 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40321 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40381 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40441 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40501 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40561 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40621 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40681 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40741 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    40801 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    40861 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    40921 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    40981 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41041 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41101 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41161 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41221 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41281 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41341 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41401 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41461 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41521 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41581 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41641 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41701 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    41761 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    41821 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    41881 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    41941 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42001 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42061 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42121 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42181 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42241 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42301 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42361 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42421 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42481 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42541 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42601 aataaacatc tttatgtaat ctgatgttct atcta
//
LOCUS       DACDID010000007        42203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDID010000007 DACDID010000000
VERSION     DACDID010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.007"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            557..1394
                     /locus_tag="IV236_000296"
                     /pseudo
     CDS             557..1394
                     /locus_tag="IV236_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            1459..1893
                     /locus_tag="IV236_000297"
     CDS             1459..1893
                     /locus_tag="IV236_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770522.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(1961..3283)
                     /locus_tag="IV236_000298"
     CDS             complement(1961..3283)
                     /locus_tag="IV236_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8770523.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3437..5077)
                     /locus_tag="IV236_000299"
     CDS             complement(3437..5077)
                     /locus_tag="IV236_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8770524.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5407..5877
                     /locus_tag="IV236_000300"
     CDS             5407..5877
                     /locus_tag="IV236_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8770525.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            6506..7609
                     /locus_tag="IV236_000301"
     CDS             6506..7609
                     /locus_tag="IV236_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8770526.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7893..8573
                     /locus_tag="IV236_000302"
     CDS             7893..8573
                     /locus_tag="IV236_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8770527.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8663..9697
                     /locus_tag="IV236_000303"
     CDS             8663..9697
                     /locus_tag="IV236_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8770528.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9711..10046
                     /locus_tag="IV236_000304"
     CDS             9711..10046
                     /locus_tag="IV236_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770529.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            10030..12510
                     /locus_tag="IV236_000305"
     CDS             10030..12510
                     /locus_tag="IV236_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770530.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            12566..14125
                     /locus_tag="IV236_000306"
     CDS             12566..14125
                     /locus_tag="IV236_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8770531.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            14122..15486
                     /locus_tag="IV236_000307"
     CDS             14122..15486
                     /locus_tag="IV236_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8770532.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15657..16727
                     /locus_tag="IV236_000308"
     CDS             15657..16727
                     /locus_tag="IV236_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770533.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16742..17410
                     /locus_tag="IV236_000309"
     CDS             16742..17410
                     /locus_tag="IV236_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770534.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            17430..18008
                     /locus_tag="IV236_000310"
     CDS             17430..18008
                     /locus_tag="IV236_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8770535.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            18411..19322
                     /locus_tag="IV236_000311"
     CDS             18411..19322
                     /locus_tag="IV236_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8770536.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(19578..20495)
                     /locus_tag="IV236_000312"
     CDS             complement(19578..20495)
                     /locus_tag="IV236_000312"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8770537.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20697..21134
                     /gene="zapA"
                     /locus_tag="IV236_000313"
     CDS             20697..21134
                     /gene="zapA"
                     /locus_tag="IV236_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8770538.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            21192..21739
                     /locus_tag="IV236_000314"
                     /pseudo
     CDS             21192..21739
                     /locus_tag="IV236_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            21854..24214
                     /locus_tag="IV236_000315"
     CDS             21854..24214
                     /locus_tag="IV236_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8770539.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            24301..24615
                     /gene="trxA"
                     /locus_tag="IV236_000316"
     CDS             24301..24615
                     /gene="trxA"
                     /locus_tag="IV236_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8770540.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24797..26575
                     /gene="uvrC"
                     /locus_tag="IV236_000317"
     CDS             24797..26575
                     /gene="uvrC"
                     /locus_tag="IV236_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8770541.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26720..27619
                     /locus_tag="IV236_000318"
     CDS             26720..27619
                     /locus_tag="IV236_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770542.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27612..28874
                     /locus_tag="IV236_000319"
     CDS             27612..28874
                     /locus_tag="IV236_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770543.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            29037..30935
                     /locus_tag="IV236_000320"
     CDS             29037..30935
                     /locus_tag="IV236_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8770544.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(31234..31953)
                     /locus_tag="IV236_000321"
     CDS             complement(31234..31953)
                     /locus_tag="IV236_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8770545.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            32290..32622
                     /locus_tag="IV236_000322"
     CDS             32290..32622
                     /locus_tag="IV236_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770546.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            32609..34612
                     /locus_tag="IV236_000323"
     CDS             32609..34612
                     /locus_tag="IV236_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8770547.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            34631..35101
                     /locus_tag="IV236_000324"
     CDS             34631..35101
                     /locus_tag="IV236_000324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8770548.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            35122..35706
                     /locus_tag="IV236_000325"
     CDS             35122..35706
                     /locus_tag="IV236_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8770549.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            35721..36707
                     /locus_tag="IV236_000326"
     CDS             35721..36707
                     /locus_tag="IV236_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8770550.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            36697..37008
                     /locus_tag="IV236_000327"
     CDS             36697..37008
                     /locus_tag="IV236_000327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8770551.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            37030..38811
                     /locus_tag="IV236_000328"
     CDS             37030..38811
                     /locus_tag="IV236_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8770552.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            38804..40180
                     /locus_tag="IV236_000329"
     CDS             38804..40180
                     /locus_tag="IV236_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8770553.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            40187..40822
                     /locus_tag="IV236_000330"
     CDS             40187..40822
                     /locus_tag="IV236_000330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8770554.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40852..>42203
                     /locus_tag="IV236_000331"
     CDS             40852..>42203
                     /locus_tag="IV236_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8770555.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        13870 a         7343 c         8968 g        12022 t
ORIGIN      
        1 aattaataag atataaaaaa agggattgtg acaaacgtct tgtcacaatc cctttttcca
       61 aataaacgat gttcaatcag ctgcccctcg gcattcgctc gaaaaacggc gaaatatgaa
      121 gaactatttt gccatttgtt tctctaataa tcaggtcaaa acgttgactt cacagtctct
      181 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
      241 gagaagctgt tttttgaaat tgttccttgt gactcggagc tgaacggcag gctcacgacc
      301 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagttcaac caaacgaaaa
      361 ttatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac
      421 aacctctttt cgtttagcac taaatttcct taaaaagtag aaaagctttc gaagcctcgt
      481 ttgtttcttt tactgtataa taaaagaaaa gagtaacaat cgctaaaaga tataaacaag
      541 agaaaggaaa tacaagatga ttggtctgat ttattttgtg gtgatcgttc tggcgaacac
      601 agttggcgct atttccggga tgggtggagg cgtgttgatc aaaccgattc ttgatcttat
      661 cggcgcccat tctgttgcag ggatttcttt ttactcaaca gttgcggtat tcacgatgtc
      721 cattgtttcg actgtgcgac aagtatcaag cgggaaatca ctgaattggc agattgtcgg
      781 ttgggtatct agtggagctg ttgttggagg agtagtcggc aacattgtat tcgaagtttt
      841 tttacagtta tttgaaaacg agaagcatgt gcagctgatc caaatttttt tgacggtatt
      901 gaccttggtt tttgcgttct tctacaccaa gcatcatcag ccaaaatttc atttgacgtc
      961 atggacttgg taccttatat gtgggggggg ttctgggctt tttagctagt tttttaggta
     1021 ttggtggtgg accagtcaat gtttctttgt tgatgctgat gtttgcattg ccaataaaag
     1081 aagcaacact atattctcta tctacgattt tcttttctca actggcaaaa ttggtaacga
     1141 ttgctttaac aagtagcttt atgcgctttg atttatctat gttgttttat gttatccccg
     1201 ctgctgttat tggaggtttg tggggagcaa gatttagcag ggttttgtct ccaaaaaaag
     1261 tgacctttat ttttcaagta attgtcctcg tggttctttt ggtcaattgt tacaatgcct
     1321 ttgtcatctt acaaacatta ggagaatcta gactaacgca agtcaaaatc actgaaacat
     1381 attgttctaa atgaaaaaaa gagtatacta tgataaaaaa attcatttta aaaaatcaga
     1441 aattggctgg tgatttggat gttacgggaa atgcgtttag aagatgcaga agcactacgt
     1501 gaaatcaatg cggatcaatt agggtatgat ataccattaa ccgttacggt gagacaaatg
     1561 aagaaactgc tgaaggagcc agagaaaaat tttttcttgg tttatgaaga tcctacttct
     1621 aaaaaagcag ctggctatgt acaagcggag tcctatgaat cgatttttag cgatccaatg
     1681 ttcaatatta tggcacttgc tgtgtctaaa tatgctgaaa aaagaggcat cggaaaggct
     1741 ttgatgactg gcgtagaaga agaagcgaaa aaacgaggaa taacgacaat aaggcttaat
     1801 tctgcagaat acagagtaga agcacaccaa ttttatgaac acatcggtta tcattccgat
     1861 aaaatgcaaa aaagattttt gaaaatcctg taaatcttta tcaaaaaaat acccgatcac
     1921 caagccgata cttgatgatc aggtattttt gtttttttga ctaagaaagt aattgcatca
     1981 agaaaccaga gatgatcaaa acgatggcac caccaagtcg attgcccatt tgagcaaaag
     2041 caataagttc catccgatta gctgcagata agacggctac atttcctgtt ccgcccatcg
     2101 agttgttgca taagccagca gtaatagttg attcaattgg atataaacca aacaaacgac
     2161 cgacaaatcc tgaaacaact gaaatcgcga taacacttgt cagacataag acaacaaatt
     2221 gccatgtcaa agcagaagct aatacattca gattcagcaa cgcaataccg ataccggcaa
     2281 gaacagctgc tgtcaagttt ttgacaatca agttattgaa catgatagct gcatcttcat
     2341 aatactctgg taataggttg gcgattttac agatgacaac gataataatc ataaatgcgt
     2401 aagcatggac tttaggaaca aaatagttac caatttgtcc taagatgaag aaagtcaatg
     2461 ataccatcaa accaacacct aactgtggaa tggaaggttt cacagttcga ggttttccat
     2521 cgccaagatc gcctttttcc attaatttac cattgccgtt gaattttggc attgattgtc
     2581 cagcacgagc aactaatgct gcaccgatga tagctaagac atttgtcatt gctgtcgcag
     2641 gaatcaggcg ggaaatgata gaagaagcat ccgtacctaa aacatttgca taaatcgttg
     2701 ataaaggaac ggctccggct ccaacaccac cagccatcgc aggtaatgag atgtataaaa
     2761 cggaatttgc aaaaccattt ccgataagca tgcccacgag tcctacggca aaaaaggaga
     2821 cgaccattga aataagtgca acgggaataa aacgaacaga agctttcatc aatagattac
     2881 ggttcatacc aaggatgcta ccagtaatca aggcagcaat ataaaagtcc caaaagccca
     2941 tattgctgac aaagttttct gtggatttca ctacatattc aggtaagatc ccaaatgtag
     3001 caatcgcagc agaagcgaag atcgcaaaaa cggaaccacc acccaaataa cttttgacga
     3061 tagggatttt tgtcccgata aaatgaaata aattaccgaa aataaccaaa actgcgattg
     3121 gtccaatcat attcgtcggt aatttcccta aagccattgc aatgatcagg acaaggacca
     3181 tgatcaaata aagtggcaaa cttacgccgg tgatttttgt ggaccaaaaa ccttcttttt
     3241 tcttttcctc aactgcagtt gcagttgttg ttttttgtgc catttttaca gatcctttct
     3301 tattctgtat catatgaaaa aagccaaaga aaagcgacaa aagagcagga gttggctggc
     3361 cgctgttttg ccgcagtcgc ttttttcttt ttagacgtca tacaaagttt ctgtatgttc
     3421 ttttactata acgatcttaa tcttctaaag atttatattc aggaacccat ttagcagatt
     3481 caactgcttc tttgatatcc gtgatttctt cacgattcaa gttttgtgcg acaactgatt
     3541 ttgcgacagt ttcagcaatc gtttgagaaa attcagtgat ttttgctact ggaggcaaga
     3601 tagctgctcc tggttttgtt acatcaacga ttccgcctaa tgctcgacta gcttgagaaa
     3661 tgatttcagc atttactcgt gtagctgttg aagcaatcaa accaagtcct aaacctgggt
     3721 acatcaaagc attatttgct tgaccgattt ggtaagttac accattatat tcaacatcag
     3781 cagcagggat acctgttcca actaatgctt ttccgtccgt ccattctatt aaatcttttg
     3841 cttttgcttc tgccaatttt gttggattag acaaaggcat gatgatcgga cgtggtgtat
     3901 gagcagccat ttctttcacg atttcttcag tgaatgctcc aggttgggta gaagtaccaa
     3961 tcatgattgt cggatggatc gcttttacaa ctgcttctaa gtttgtcaat tcatcagcat
     4021 tagtgaaatc tgagcgtttg cgagcgaatg ggatttgacc aggagtcaat ccttctgtat
     4081 cttcaaacaa taaaccttgt ttatctactt gatagaaatg ttggcgtgct tcttcttcag
     4141 gtacaccttg acggatcatc tcatctaaaa ggatgtttgc gatacccaca ccagcagtac
     4201 cagcaccaaa tgtcaaaatt atttgatctt tcaattgttc accggatata ttcaatccgc
     4261 ctaatacacc tgctaggaca acaattcctg taccttggat atcgtcgttg aaagtagtga
     4321 tttgatcttc gtatttttct aagatcgctg cagcatttcc tcgtccaaaa tcttcccaat
     4381 gcaataacaa ttctgggaat aattcggtag ctgattgaac gaattgatcg atgaattcat
     4441 agtaagtttc gccttctaca cgagcatgac gattgcctaa gtataaggga ttattcaata
     4501 attcttcatt attagtacca gcgtcgatcg atacaggcaa aacttgtgct ggattaatac
     4561 cagcagcagc agtatagacc atcaatttgc cgatagcgat gtcaacaccg ttcacacccc
     4621 agtcgcccat acctaagatt ccttctgcat cagtcacaac aatcaaacga atgtctcgtc
     4681 catctgccgc gttcttcaaa cttgctttga tgttttctgg ttcgtcgatt gaaagaaatg
     4741 cagcatcttg cggtttcaag aaaatttcgt tgtattgttc gattgaatcc gcaacgacag
     4801 gatcataaac gacaggcatg aattcaacta aatgttcgcc catcaattta taaaacaatg
     4861 tccgattcgt attgaataga ttcattaaga agatacgttt ttccaaatct gtttgtttgc
     4921 ttaagtattg accataagct tgaacagatt gttgttccag tgtttggaca gtacttggaa
     4981 gcataccagt gattccatat tttgcccgtt cttcttttgt aaaagctgtt cctttattta
     5041 gaaaaggatt atttaataag tttaaccctg taagcatgaa tgattcctcc taagttttaa
     5101 tacaaacgaa gtatagaagt cagatgatga tatagtcaaa aagagcaggt atgataaatt
     5161 ttatttatat gtaatatagc caaaagaaat caaaatagca gtatttgttc tttttatatc
     5221 gacaatcctt gcttgaatac tatgcgtatc tcttatatgc tagagaaaga aaagtagaag
     5281 aataagggat agatggaatg gataaatagg atttctgagg aaaaaagcac atgatagcgt
     5341 tgataggagt cacctatgtc aggtgataaa aatacataag tagaaaatta agaggaggaa
     5401 gagaaaatga aagaagttca attgattaga aaaagcgaac tatcagaagg cggttgcaat
     5461 gcatgtggcg tagtagaagc gacaagctat acattaaaat tagattccaa tcaagcaata
     5521 atctctgaac tgacagtagg cggtcttgtg gacagtctgg cgcttgctga aggttttact
     5581 ggagaagata tttatgagat gttttcagaa gtccgtcagt taaaaaaagg agaaaaatgc
     5641 atagaggttc atcatgagag cccgaatgtg aggttcaaac gtggtgataa tgagatgatc
     5701 ttcaagaatc atgtgtctga tcacactgaa ctatatgaaa tcgtgaatca aatactgtct
     5761 gggatttttg aattaggacc ttatgagttc aaggaagaaa acggtaatcc aaaattaaat
     5821 gaagaatggc aagaaacgat tgaaacacaa agaaacaatc cacacttatt tcaatagtct
     5881 tccacagtat ttcgtaaaaa tgttgatgaa aaagaaacac cgtgaatcag aggtcgtgaa
     5941 aaacctgccc tgcatcaagt aataagaaca gccaaacaaa aatagctcct cctatttttc
     6001 gtttggttgg gcttattacc gcagatgcaa gtttttgaac accgtttaag gaagagactg
     6061 tgaagtcaac gttctgacct gagtattaga gagacaaatg gcaaaatagt tcctcatatt
     6121 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgtttt atcgagaggt
     6181 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
     6241 tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt ttattcaaaa
     6301 aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta tttttattac
     6361 aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag attatttgcc
     6421 tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt tcatcattca
     6481 aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta caaatgaagg
     6541 aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag tgattccagc
     6601 tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc aagaaaatga
     6661 acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag aattacccga
     6721 tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg caccagttgc
     6781 tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg tagcagattt
     6841 tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga taagagaagc
     6901 aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata atggaatcaa
     6961 tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat tgacagcgga
     7021 tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct tcccgctgcc
     7081 aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg ctgtatataa
     7141 atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc attctgattt
     7201 gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat tagaatctgg
     7261 tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg ggcctgctgc
     7321 gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa tcgtgtttga
     7381 cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg ccgatcttgt
     7441 tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag gagtccatca
     7501 agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg gaacacaaac
     7561 agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag aaataaatga
     7621 cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt tcaaggatgt
     7681 cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca tacttaataa
     7741 aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat acaaaaaggg
     7801 tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt ctctactata
     7861 cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt gggacattat
     7921 tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag ggtcaagcga
     7981 cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct cttaacccta
     8041 ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact ggccagacag
     8101 gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag attgaaagcg
     8161 gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc caagtatcag
     8221 atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca gtttcggttt
     8281 cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt ggaacatcca
     8341 ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact gtaactgggt
     8401 tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa ggtctgggaa
     8461 catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa gcagcagctg
     8521 gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca tagagtatga
     8581 aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta ccgcagttgt
     8641 tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt cgtttttttc
     8701 tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt cagcaggaga
     8761 ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg gttattttga
     8821 tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca attcttcaga
     8881 tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg gtggaacgat
     8941 cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg tccgtgacgt
     9001 tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa ctatacctat
     9061 agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta ttcatgtttc
     9121 acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca aaaatcgtat
     9181 tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac cggatctgaa
     9241 acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca
     9301 aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct tttatgaaaa
     9361 tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt cgaattttca
     9421 cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg cagaaatcat
     9481 agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga tcaaaaaaga
     9541 aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat ctttcccatg
     9601 gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc tgtttttgtg
     9661 ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg ttgaaaacat
     9721 cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa gagccagtcc
     9781 aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca gttccgcatc
     9841 caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt tataaagaat
     9901 ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt ctttatttga
     9961 tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg
    10021 aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt ttttacttcg
    10081 ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg agcaacgcct
    10141 cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa tggtggacgt
    10201 agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg
    10261 actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac ggtccgtttt
    10321 cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat cactgcacaa
    10381 ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag tgatgtatta
    10441 aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat caaggttact
    10501 agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg gtcgtgggat
    10561 acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt caacgtggac
    10621 gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga aaacggagat
    10681 aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc ttctatgtct
    10741 aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca agatcgtatg
    10801 ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac tggcggaaac
    10861 ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg gaatagttac
    10921 atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac agaatacgtg
    10981 gatggaaata accagaacct tccaggctct accaatagtc ggacattgac gatggggctg
    11041 actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt caactatttt
    11101 acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag cacaagtgga
    11161 ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg attgctttct
    11221 cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt accaacaaac
    11281 ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca agtcacggcc
    11341 aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta ttcgaaagga
    11401 acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca atcaaataac
    11461 gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca atattatttt
    11521 gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc gtacaaatgg
    11581 cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa ttttccatct
    11641 gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg agatcccctg
    11701 tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac aaaatctggg
    11761 gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac ttatatcaat
    11821 ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca aggagtttct
    11881 gtcccagcag atctattgag caaaatagga gataccattc atcttactta caactatcaa
    11941 ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc agctgtactt
    12001 tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt ccaaacatct
    12061 gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc agatgatttt
    12121 acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc aaaaggcgat
    12181 ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca actgacaggg
    12241 acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac aaaattatac
    12301 ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt gatttatgag
    12361 agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga tggtttactc
    12421 ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca
    12481 tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga tctgtgataa
    12541 ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa aatcagaaat
    12601 gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac aacggcaaat
    12661 caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata acgtacttac
    12721 ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac aagctaactt
    12781 ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat taatcagaca
    12841 tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt ctacaaaata
    12901 tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga
    12961 acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg attctttaaa
    13021 tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc aaatttgtta
    13081 tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa aagaatacag
    13141 tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt ttactattac
    13201 agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc gaatgcgctg
    13261 tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc aaacgacaca
    13321 atttttattg gacctccata catttttgac ccgttacttc tttcaatatg cctttccttg
    13381 gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta cattagatac
    13441 acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg acgtagtagc
    13501 tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt atgtttttcc
    13561 agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc attttgccta
    13621 ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt taactgaagt
    13681 agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat ttgctattgt
    13741 tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc
    13801 tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt atgatcttcc
    13861 ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat tgctgaatac
    13921 gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca ttttgactag
    13981 ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca ctagcaacga
    14041 atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt tagaaaaatt
    14101 gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca tgcaggcatc
    14161 acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact gatcgatgaa
    14221 gatcataaag atggactagg ctatgtttca aatggaatca acctttattt aaaaggaaag
    14281 atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag tggggcaaaa
    14341 ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct catcctcgca
    14401 tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc aacaggttcc
    14461 tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta taaaaatcta
    14521 gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt gatcttcggt
    14581 gctggttata ttggattgga attagcagat gctttgagaa ataaaggata cattattcat
    14641 ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat gatcaacagt
    14701 tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt cttgatcgat
    14761 tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc aataaaggca
    14821 gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa agaaaaagtg
    14881 gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag tgatccggac
    14941 atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg gcatctcttt
    15001 ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac tttgagtggt
    15061 cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat agactacttt
    15121 ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa taccggttcg
    15181 tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac agtaaatgca
    15241 aagctgatct accatccgga tacattagaa ataattggcg gacagctgat aagccaagaa
    15301 ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac tacgattcca
    15361 cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt tcactacctc
    15421 aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt agataaaaga
    15481 ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta atcaatcgag
    15541 aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata ttcgccaaaa
    15601 atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg agttaaatgt
    15661 ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt gcaggggtga
    15721 tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc ttagctcaag
    15781 ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg aaagatgcaa
    15841 attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc gaaggtggga
    15901 aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt acagaagaag
    15961 gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg cccaatcttg
    16021 ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt ttgaaagaag
    16081 aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag acgtttaaac
    16141 taactggctt tacggatcat gccaaattca atgtctcacc agttctctat acaaccatca
    16201 atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca cggattaacg
    16261 caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag atcaaaattt
    16321 ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt ggatttatga
    16381 tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat gtactgacga
    16441 tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga ggatttattg
    16501 cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt cttggtctat
    16561 taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa agcaattggc
    16621 tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc gcactatttt
    16681 ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa ggaggaagag
    16741 catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg accaagtagt
    16801 taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg caatcatcgg
    16861 tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc agacgcctag
    16921 cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa gtgaacgcgt
    16981 taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg ttccttttct
    17041 gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc gtcaaaaaga
    17101 agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt atccagaaga
    17161 actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc
    17221 cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac gagaagtagt
    17281 cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg ttactcacga
    17341 tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg tattgaaaga
    17401 aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa gatacacagc
    17461 aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc ggcacttcaa
    17521 caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat tatttcggtg
    17581 ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt ggaaaagctt
    17641 tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc
    17701 agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat cttgaaagca
    17761 atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat tatcgtgaac
    17821 cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa
    17881 tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg aaaagacctg
    17941 tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt gtattaggag
    18001 aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag atatgacgaa
    18061 gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa tttgttattc
    18121 tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa
    18181 tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga
    18241 atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt
    18301 agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc gtacttaagc
    18361 tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa
    18421 taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt
    18481 ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga
    18541 caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc
    18601 gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc
    18661 tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata
    18721 ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg
    18781 aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg
    18841 cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag
    18901 taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat
    18961 tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca
    19021 gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt
    19081 actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc
    19141 tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt
    19201 ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag
    19261 acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt
    19321 aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat
    19381 tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc tgccgtttaa
    19441 ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac
    19501 ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac tgtgacatga
    19561 cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt attggcaaaa
    19621 tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag ttttgccgct
    19681 acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc agctgatgct
    19741 ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc aacagctaaa
    19801 tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt tttttctaaa
    19861 cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat cgcttctggt
    19921 ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa agcgactttc
    19981 atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga taagacaagc
    20041 agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg gctatctttt
    20101 aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc acgtttcaca
    20161 taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc atcactacct
    20221 aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc tggcttggct
    20281 tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt accttgaaac
    20341 atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta
    20401 gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat ttgactaagt
    20461 tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt tttatacata
    20521 aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca tagcattttt
    20581 ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga
    20641 accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg attcgtatgg
    20701 cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact attatcggac
    20761 gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag ttagcagaat
    20821 tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg gctgtcaatg
    20881 cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa gaaacagcag
    20941 aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag
    21001 caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat cataccatta
    21061 aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa attaaacaaa
    21121 aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa
    21181 aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg
    21241 gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta
    21301 ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac attccatacc
    21361 cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct tttcacctag
    21421 atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta
    21481 cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag
    21541 actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt
    21601 tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg agcatagttt
    21661 cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt
    21721 aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt
    21781 agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag
    21841 ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca
    21901 gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc
    21961 agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt
    22021 gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc cccatatgaa
    22081 acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt
    22141 gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt
    22201 tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt
    22261 aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag
    22321 cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt
    22381 ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta
    22441 cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag
    22501 tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt
    22561 gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc
    22621 tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg gtaaaatgga
    22681 tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag
    22741 tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt
    22801 agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa
    22861 cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc
    22921 aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc
    22981 ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac
    23041 gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct
    23101 cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct
    23161 tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg
    23221 atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata cactaagtcc
    23281 aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg
    23341 attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca
    23401 agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata
    23461 tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc
    23521 ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa
    23581 cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat
    23641 gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca
    23701 attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa
    23761 agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg
    23821 gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa
    23881 tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat
    23941 ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa
    24001 acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg
    24061 ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac
    24121 tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg
    24181 cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt
    24241 cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga ggtctttgat
    24301 atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg
    24361 attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag
    24421 ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat
    24481 ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat
    24541 ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc
    24601 gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc
    24661 cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa
    24721 ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt taattgaata
    24781 tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca
    24841 gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa
    24901 aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga aaacggaacg
    24961 gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt
    25021 gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga
    25081 tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac
    25141 tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc
    25201 gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca
    25261 gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt attatttcga
    25321 cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg
    25381 acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga
    25441 gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg
    25501 ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg cgattgacaa
    25561 aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt
    25621 ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt
    25681 ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa
    25741 gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa
    25801 aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac ggtttgatct
    25861 gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa
    25921 tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc
    25981 tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata
    26041 taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg
    26101 cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg
    26161 aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat
    26221 tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc tattcggtat
    26281 agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat
    26341 ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag
    26401 ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct
    26461 taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat
    26521 cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa
    26581 gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt
    26641 acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc
    26701 ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta
    26761 cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg
    26821 ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga
    26881 ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg
    26941 ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta
    27001 ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg atttttggat
    27061 ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa
    27121 tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga
    27181 tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga
    27241 attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa
    27301 aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca
    27361 tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca
    27421 ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga
    27481 gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg
    27541 ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc
    27601 aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta
    27661 agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg
    27721 tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata
    27781 atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag
    27841 ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag
    27901 tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac
    27961 aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcaa
    28021 gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc
    28081 gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat aactcgacag
    28141 tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg
    28201 cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc
    28261 tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt ttacttgttg
    28321 cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag
    28381 atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat
    28441 tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag
    28501 ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga
    28561 cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca gatccaaaca
    28621 atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt atgccggttc
    28681 gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag
    28741 ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgtgt
    28801 atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg agaaatgaac
    28861 gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat tgttcccagc
    28921 ttcttttttg tatagaccta tttctatttt tttattatat tggatatata tattcattga
    28981 aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc gttttaatgg
    29041 accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc atcacggaaa
    29101 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagcgata
    29161 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
    29221 aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc caattcacta
    29281 tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa caagataaat
    29341 cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa tacactcgat
    29401 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
    29461 tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa caacttgtct
    29521 tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa aaatctgtca
    29581 tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca gccaaagtaa
    29641 tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat gatattttgt
    29701 ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg attgccgcac
    29761 gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt ccattatttt
    29821 cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac aggaaagatc
    29881 tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct atcctccaat
    29941 tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac gatcgtaaat
    30001 tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt
    30061 tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc ttggaagagg
    30121 aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga atgcaaaaac
    30181 acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtactgt
    30241 tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa ttagaccaat
    30301 cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag
    30361 atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg agcgggccat
    30421 tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt ctcacagagc
    30481 ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa gagtggttca
    30541 gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac
    30601 acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga aaagcatttg
    30661 ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct ggctactcat
    30721 tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg cctattgaac
    30781 aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc
    30841 cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag attactcaac
    30901 tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca acattgctca
    30961 atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa
    31021 aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc aggtcagggc
    31081 gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc
    31141 acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagttg
    31201 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
    31261 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
    31321 gattttgtgc atattatctt tactttgtac tgcttcaatg agtccatcgt tggaaaaggc
    31381 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
    31441 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
    31501 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
    31561 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
    31621 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
    31681 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
    31741 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
    31801 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
    31861 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
    31921 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
    31981 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
    32041 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
    32101 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
    32161 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
    32221 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
    32281 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
    32341 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
    32401 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
    32461 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
    32521 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
    32581 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
    32641 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
    32701 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
    32761 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
    32821 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
    32881 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
    32941 catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc taactgaaaa
    33001 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
    33061 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
    33121 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
    33181 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
    33241 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
    33301 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
    33361 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
    33421 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
    33481 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
    33541 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
    33601 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
    33661 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
    33721 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
    33781 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
    33841 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
    33901 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
    33961 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
    34021 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
    34081 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
    34141 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
    34201 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
    34261 tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
    34321 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
    34381 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
    34441 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
    34501 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
    34561 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
    34621 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
    34681 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
    34741 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
    34801 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
    34861 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
    34921 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
    34981 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
    35041 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
    35101 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
    35161 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
    35221 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
    35281 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
    35341 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
    35401 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
    35461 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
    35521 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
    35581 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
    35641 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
    35701 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
    35761 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
    35821 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
    35881 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
    35941 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
    36001 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
    36061 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
    36121 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
    36181 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
    36241 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
    36301 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
    36361 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
    36421 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
    36481 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
    36541 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
    36601 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
    36661 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
    36721 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
    36781 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
    36841 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
    36901 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
    36961 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
    37021 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
    37081 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
    37141 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
    37201 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
    37261 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
    37321 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
    37381 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
    37441 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga
    37501 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
    37561 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
    37621 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
    37681 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
    37741 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
    37801 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
    37861 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
    37921 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
    37981 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
    38041 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
    38101 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
    38161 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt
    38221 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
    38281 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
    38341 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
    38401 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
    38461 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
    38521 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
    38581 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
    38641 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
    38701 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
    38761 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
    38821 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
    38881 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
    38941 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
    39001 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
    39061 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
    39121 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
    39181 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
    39241 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
    39301 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
    39361 cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
    39421 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg
    39481 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
    39541 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
    39601 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
    39661 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
    39721 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
    39781 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
    39841 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
    39901 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc
    39961 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
    40021 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
    40081 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
    40141 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
    40201 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
    40261 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
    40321 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
    40381 ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc
    40441 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
    40501 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
    40561 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
    40621 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
    40681 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
    40741 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
    40801 aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa
    40861 caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt
    40921 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
    40981 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg
    41041 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
    41101 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
    41161 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    41221 tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat ccaattgatt
    41281 ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg
    41341 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    41401 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    41461 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    41521 atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    41581 gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa
    41641 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    41701 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc
    41761 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    41821 cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga
    41881 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    41941 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    42001 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    42061 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    42121 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    42181 ccagaagaaa gtgtcatgat tgg
//
LOCUS       DACDID010000008        42044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDID010000008 DACDID010000000
VERSION     DACDID010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.008"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..497)
                     /locus_tag="IV236_000332"
     CDS             complement(99..497)
                     /locus_tag="IV236_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8770556.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(494..1267)
                     /locus_tag="IV236_000333"
     CDS             complement(494..1267)
                     /locus_tag="IV236_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8770557.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1264..1947)
                     /locus_tag="IV236_000334"
     CDS             complement(1264..1947)
                     /locus_tag="IV236_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8770558.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2114..3001)
                     /locus_tag="IV236_000335"
     CDS             complement(2114..3001)
                     /locus_tag="IV236_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8770559.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2998..3867)
                     /locus_tag="IV236_000336"
     CDS             complement(2998..3867)
                     /locus_tag="IV236_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8770560.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4004..5290)
                     /locus_tag="IV236_000337"
     CDS             complement(4004..5290)
                     /locus_tag="IV236_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770561.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5563..6141
                     /locus_tag="IV236_000338"
     CDS             5563..6141
                     /locus_tag="IV236_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8770562.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6141..7070
                     /locus_tag="IV236_000339"
     CDS             6141..7070
                     /locus_tag="IV236_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770563.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7067..8803
                     /locus_tag="IV236_000340"
     CDS             7067..8803
                     /locus_tag="IV236_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8770564.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8866..9702)
                     /gene="pheA"
                     /locus_tag="IV236_000341"
     CDS             complement(8866..9702)
                     /gene="pheA"
                     /locus_tag="IV236_000341"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8770565.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9699..10208)
                     /locus_tag="IV236_000342"
     CDS             complement(9699..10208)
                     /locus_tag="IV236_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8770566.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10201..11496)
                     /gene="aroA"
                     /locus_tag="IV236_000343"
     CDS             complement(10201..11496)
                     /gene="aroA"
                     /locus_tag="IV236_000343"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8770567.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11509..12600)
                     /locus_tag="IV236_000344"
     CDS             complement(11509..12600)
                     /locus_tag="IV236_000344"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8770568.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12617..13783)
                     /gene="aroC"
                     /locus_tag="IV236_000345"
     CDS             complement(12617..13783)
                     /gene="aroC"
                     /locus_tag="IV236_000345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8770569.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13785..14849)
                     /gene="aroB"
                     /locus_tag="IV236_000346"
     CDS             complement(13785..14849)
                     /gene="aroB"
                     /locus_tag="IV236_000346"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8770570.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14882..15904)
                     /gene="aroF"
                     /locus_tag="IV236_000347"
     CDS             complement(14882..15904)
                     /gene="aroF"
                     /locus_tag="IV236_000347"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8770571.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16563..17537)
                     /locus_tag="IV236_000348"
     CDS             complement(16563..17537)
                     /locus_tag="IV236_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8770572.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17518..19401)
                     /locus_tag="IV236_000349"
     CDS             complement(17518..19401)
                     /locus_tag="IV236_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8770573.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19419..21644)
                     /locus_tag="IV236_000350"
     CDS             complement(19419..21644)
                     /locus_tag="IV236_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8770574.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21759..22793
                     /locus_tag="IV236_000351"
     CDS             21759..22793
                     /locus_tag="IV236_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8770575.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22860..23873)
                     /locus_tag="IV236_000352"
     CDS             complement(22860..23873)
                     /locus_tag="IV236_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8770576.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23951..24139)
                     /locus_tag="IV236_000353"
     CDS             complement(23951..24139)
                     /locus_tag="IV236_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8770577.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24579..24707)
                     /locus_tag="IV236_000354"
     CDS             complement(24579..24707)
                     /locus_tag="IV236_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8770578.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24710..26083)
                     /gene="rlmD"
                     /locus_tag="IV236_000355"
     CDS             complement(24710..26083)
                     /gene="rlmD"
                     /locus_tag="IV236_000355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8770579.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26194..27249)
                     /locus_tag="IV236_000356"
     CDS             complement(26194..27249)
                     /locus_tag="IV236_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8770580.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27259..28689)
                     /gene="gatB"
                     /locus_tag="IV236_000357"
     CDS             complement(27259..28689)
                     /gene="gatB"
                     /locus_tag="IV236_000357"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8770581.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28686..30158)
                     /gene="gatA"
                     /locus_tag="IV236_000358"
     CDS             complement(28686..30158)
                     /gene="gatA"
                     /locus_tag="IV236_000358"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8770582.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30158..30463)
                     /gene="gatC"
                     /locus_tag="IV236_000359"
     CDS             complement(30158..30463)
                     /gene="gatC"
                     /locus_tag="IV236_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8770583.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30569..32605)
                     /gene="ligA"
                     /locus_tag="IV236_000360"
     CDS             complement(30569..32605)
                     /gene="ligA"
                     /locus_tag="IV236_000360"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8770584.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32622..34856)
                     /gene="pcrA"
                     /locus_tag="IV236_000361"
     CDS             complement(32622..34856)
                     /gene="pcrA"
                     /locus_tag="IV236_000361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8770585.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35011..36570
                     /locus_tag="IV236_000362"
     CDS             35011..36570
                     /locus_tag="IV236_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8770586.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36681..38612)
                     /locus_tag="IV236_000363"
     CDS             complement(36681..38612)
                     /locus_tag="IV236_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8770587.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38630..39544)
                     /gene="pfkB"
                     /locus_tag="IV236_000364"
     CDS             complement(38630..39544)
                     /gene="pfkB"
                     /locus_tag="IV236_000364"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8770588.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39541..40293)
                     /locus_tag="IV236_000365"
     CDS             complement(39541..40293)
                     /locus_tag="IV236_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8770589.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40452..40661)
                     /locus_tag="IV236_000366"
     CDS             complement(40452..40661)
                     /locus_tag="IV236_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770590.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40672..42000)
                     /locus_tag="IV236_000367"
     CDS             complement(40672..42000)
                     /locus_tag="IV236_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770591.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        11626 a         9131 c         7560 g        13727 t
ORIGIN      
        1 ctatttcata agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg
       61 ccttcttgtt tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta
      121 tcgttatttt catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta
      181 gtgataccaa tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg
      241 atgttgatgc cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat
      301 gtcaacacaa tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt
      361 acaccagaac ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag
      421 tcgataacat cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat
      481 gctacaagat ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt
      541 ctttttcatc ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca
      601 atgcaatcat tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg
      661 cagcgattgc cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc
      721 caccgttatc aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg
      781 ctagcccgta tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca
      841 tagacaatcc tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc
      901 ggattctccc ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag
      961 caaaccctac taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca
     1021 atcccgcaat agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt
     1081 gcaggaaaag gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac
     1141 gctttttgcg catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg
     1201 ccactgccaa aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga
     1261 aactcattgg aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat
     1321 accgaccatt ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc
     1381 tccggcaagg gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc
     1441 aaaagcaatc ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta
     1501 gcctatagcc atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc
     1561 tagaaatact ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc
     1621 gtcagatata tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat
     1681 cacgatcccg atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat
     1741 atatttccct actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag
     1801 cgtcattccg atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc
     1861 taacggtgca aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc
     1921 aatcacttct ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt
     1981 aaaatgaaag agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata
     2041 aaataacaca cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc
     2101 ttattgtgtt tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa
     2161 caaaaaatac gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat
     2221 agtctggtgt ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag
     2281 gagattgcaa agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga
     2341 ttgcagccgc ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta
     2401 gttcttttag accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat
     2461 tttgtcgaaa gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga
     2521 aactgttgat tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca
     2581 tcaatgccgc aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc
     2641 gtttactttg ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca
     2701 cagtggtcac caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat
     2761 tggaaaacaa attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg
     2821 tgatatccac gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa
     2881 taaatgatag gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt
     2941 ctaatgcttg ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca
     3001 tcgactttct cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat
     3061 aacaaaggaa actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata
     3121 gatgtattga gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac
     3181 ttcatcaaaa agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg
     3241 ttttaaattt gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga
     3301 tgctgcttca taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt
     3361 gtatccagtc caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg
     3421 agtcaaccag ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag
     3481 gccagattga gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca
     3541 tggaagaaag atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag
     3601 gttggaaata aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa
     3661 tgtattaaaa aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa
     3721 cccgttaaat gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac
     3781 catcggaata aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc
     3841 gtttttgaat aaatctttct gcttcattct tttccctcca attctatcat tgattatcta
     3901 catgaaaaga caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt
     3961 ttgtctgatg aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg
     4021 cttctacttg tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc
     4081 gatgaagagc ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt
     4141 ctggtatttc agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt
     4201 cagaagcttc ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga
     4261 tttctttcgc aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat
     4321 ctttcgctga atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt
     4381 tagaagcttg cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt
     4441 ttccagattg gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag
     4501 aagctactgc tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga
     4561 ctagatcttc tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt
     4621 cttggatcgt gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa
     4681 tcatccttac tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt
     4741 ctttgacatc tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt
     4801 ctgtataacc tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat
     4861 cgaaaggaac accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa
     4921 aatgatcttc ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat
     4981 agccttgtat cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc
     5041 tcgctaaagc ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta
     5101 tctctgtttc ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg
     5161 tctcatccca cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc
     5221 cgcatcctgt taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc
     5281 tgatgttcat ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca
     5341 tcataccaaa atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa
     5401 aataataaaa aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa
     5461 gatcgaaatg gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa
     5521 gtatttttaa attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt
     5581 cttatcgatg atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag
     5641 cacgggtttc aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg
     5701 gtacatgcaa tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa
     5761 ttatcgcggc tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat
     5821 gatgaatttg atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag
     5881 cctcgattga gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa
     5941 aaaaagccca acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca
     6001 cagcagattg ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg
     6061 tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac
     6121 agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta
     6181 ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt
     6241 ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt
     6301 ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac
     6361 gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca
     6421 accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag
     6481 ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt
     6541 atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc
     6601 atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg
     6661 ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact
     6721 tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc
     6781 aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat
     6841 atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa
     6901 aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg
     6961 aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag
     7021 agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat
     7081 caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca
     7141 agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt
     7201 caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga
     7261 tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc
     7321 caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa
     7381 tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa
     7441 tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca
     7501 atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca
     7561 ccacggattg actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa
     7621 aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc
     7681 gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga
     7741 acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa
     7801 aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct
     7861 gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat
     7921 gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt
     7981 ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa
     8041 acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca
     8101 attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac
     8161 gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca
     8221 gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac
     8281 agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa
     8341 aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct
     8401 tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca
     8461 gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc
     8521 ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct
     8581 tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa
     8641 tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg
     8701 cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg
     8761 tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg
     8821 atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc
     8881 ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat
     8941 ttcttggagc gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa
     9001 aaaatattct cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc
     9061 ccgccagcca aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt
     9121 caagatgaca ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac
     9181 gatccaaaaa cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa
     9241 tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc
     9301 tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata
     9361 atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt
     9421 aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt
     9481 aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa
     9541 tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca
     9601 aacagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg
     9661 ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct
     9721 ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca
     9781 ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt
     9841 ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc
     9901 gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca
     9961 ctttggacaa tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa
    10021 atatctgact ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc
    10081 gacaattgga tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat
    10141 tgagctaacc ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg
    10201 ctagccatga gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata
    10261 agagattgaa acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc
    10321 tacttgcagc atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag
    10381 aggtgtcggc ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt
    10441 ctgtaattct tctgccaccg catcaattct gttggtttct tttactttta attcttctgc
    10501 gtctttgatg atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc
    10561 atcaatcaga cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt
    10621 gacacttaaa tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc
    10681 catctcctcc aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa
    10741 caacagttgg ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat
    10801 atcacctgga acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt
    10861 tttatcttcc actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg
    10921 ggaacgttct ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa
    10981 agcagatttg acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg
    11041 ggttcctgtc acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc
    11101 catctcgcgt aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc
    11161 gaacagagta gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt
    11221 accggagttt cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc
    11281 atgaatcttg atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg
    11341 taaagtactc agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc
    11401 aattgctcca aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt
    11461 ccctcttaat ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca
    11521 gcaatgataa ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt
    11581 tttaaaactg atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt
    11641 aatcaaagaa actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc
    11701 atctggcaca tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg
    11761 caactggtct cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc
    11821 agtcatgatc cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa
    11881 taatatctgc ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat
    11941 cctagtgata tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt
    12001 gaattgttct gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat
    12061 tcctgttata cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca
    12121 aaatagttct tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta
    12181 ataggcattt tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc
    12241 cattggatgt ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg
    12301 cttcgtactg ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac
    12361 atcaagataa gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc
    12421 tgcacctgtt tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc
    12481 acgggttttt gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat
    12541 acaaagtgcc atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat
    12601 cttatttgcc ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt
    12661 tcaactcaac aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg
    12721 ctacaacatt ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta
    12781 cacttgcttt gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg
    12841 taggaatggg tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt
    12901 cgaagccgcc tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct
    12961 catccatcac ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc
    13021 ctttaaaggc attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc
    13081 attgtacgta gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc
    13141 cgatcgtatc tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg
    13201 ctggatcaac aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga
    13261 tatttccagg tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga
    13321 tgtcaagttc ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct
    13381 ctcttgcaga ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc
    13441 ctaccaaatc cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt
    13501 caggcacttc tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca
    13561 ctaaagtgac aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac
    13621 gatctttttc gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt
    13681 cgtgattgat ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg
    13741 ttaattctgg tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag
    13801 taatctttga atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc
    13861 aagatgatat tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat
    13921 tcatctggat gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct
    13981 aaagcagctg tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct
    14041 accattccaa tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca
    14101 attgcatggc caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca
    14161 tcttcttcca ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt
    14221 tccagaagat ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct
    14281 atagctgctg atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt
    14341 tttagaacct caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt
    14401 ttcgctttgt ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt
    14461 agcgtagtcg gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct
    14521 gctagatcac ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca
    14581 ttatctgcta aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc
    14641 gccggtacga ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt
    14701 tctgcatata gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa
    14761 atcgtttgaa gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat
    14821 ttttggttat caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga
    14881 attaatcgag tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca
    14941 tgcgtgagta agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat
    15001 caggatggat ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg
    15061 gtggtacaag ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag
    15121 ttaatttttt catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt
    15181 aagtccgaat cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt
    15241 ctgcagcatt aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt
    15301 tacctgtttt accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct
    15361 gtaggatatc gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa
    15421 tcactttcat tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta
    15481 aaccttggaa agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg
    15541 cgcccccggc tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac
    15601 aaggaccagc catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga
    15661 ctgtattgcc aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag
    15721 cattttctac tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt
    15781 ttagtccaat cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt
    15841 cgatatgatc gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta
    15901 tcattgtttt tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt
    15961 ttcaaggtgg ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg
    16021 ctccttcttt cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat
    16081 tgcctctaca gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc
    16141 cactatagat gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat
    16201 agatacaaac ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag
    16261 ctaaagtaat acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa
    16321 ctgatgtaag aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg
    16381 aaactttttt taattttctg atagttaact ttgtttttca gaaaaataac aattgatttt
    16441 attgaagaaa atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga
    16501 catatagaat aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat
    16561 tgctactgaa taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac
    16621 tcaacatccg ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta
    16681 agaaccgttc gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct
    16741 gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa
    16801 aaatcttctt gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat
    16861 ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct
    16921 gccactttat aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat
    16981 aggccttcat actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg
    17041 ggtaattccg gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct
    17101 ccagcgattt ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta
    17161 aaaatgattt caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca
    17221 atcggcaaat cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac
    17281 aatccggctc ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg
    17341 gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta
    17401 ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg
    17461 acacctaatg tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta
    17521 tttactgtat ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc
    17581 tgcaaaaacg atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc
    17641 gccatagcgc atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt
    17701 aaaatcccat atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga
    17761 atccattcca cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt
    17821 ttccgcttta ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt
    17881 tacatctgaa atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag
    17941 tcgatcgggg tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata
    18001 agattcattt ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt
    18061 cgtttttgct cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta
    18121 agaaggctca aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc
    18181 catcacatgg attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg
    18241 agatgtccgt acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc
    18301 ttctatagtg atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat
    18361 aatcatccgt tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc
    18421 gaaggaatag tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag
    18481 cttatataaa ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg
    18541 aatcgtcatt tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc
    18601 tagtatcgct acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc
    18661 aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg
    18721 gttttgtcct cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc
    18781 ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc
    18841 ttcttccaaa taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc
    18901 gataaaatga ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga
    18961 gatttgtttt ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg
    19021 attgtcttta gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg
    19081 gggacggcga aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc
    19141 ataatttgct aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa
    19201 atctttttta taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca
    19261 tgatccattt aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata
    19321 acaaacatga tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa
    19381 ccattttata tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc
    19441 agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg
    19501 gcccccgaaa atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc
    19561 atcagatata cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca
    19621 atagcttcat cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga
    19681 ctcgccaaac gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata
    19741 atttggtctt ttacttgctt tttttcttca ggagacagct ttgtcaaatc cagttcataa
    19801 ccaaaaacag cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc
    19861 tgatgatttg gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt
    19921 gttccatatt ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc
    19981 ggcatataat aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat
    20041 aagatttccg gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa
    20101 cgatgcgcta cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc
    20161 gaacggttca tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt
    20221 tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg
    20281 gaagcagaag gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt
    20341 tcagaatcaa ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta
    20401 tgagcataat ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag
    20461 tctcctaatg aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac
    20521 atttcgatgc ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag
    20581 tcaaagtaag tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga
    20641 cctcttgcga ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc
    20701 tgacttgagt aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc
    20761 cacgaaaaac gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt
    20821 tcaagttcga aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta
    20881 ttttctgtcg tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt
    20941 cctcgacggc tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt
    21001 tcattgacat gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag
    21061 tctagctgga aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc
    21121 tttacagatc gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa
    21181 atcgctgttt ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc
    21241 acataagttt gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca
    21301 tatctcagat ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga
    21361 aagtccatgc tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg
    21421 tcaggattta agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg
    21481 tctttgtact gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct
    21541 atctttatca gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca
    21601 aataagatga aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca
    21661 aaacggaaac cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt
    21721 caataaaaat tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg
    21781 cacgaaaggc aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga
    21841 tccatgtggc tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg
    21901 acatcagtac tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga
    21961 tcggcaaagc gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag
    22021 aagaagctcg acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta
    22081 aacaatggga agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag
    22141 aatcgattac cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca
    22201 tccagcaaga ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg
    22261 aattgttctt agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata
    22321 tggatgaatt cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc
    22381 attttatgat cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag
    22441 aaaaacaagg tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg
    22501 ccattcttgt cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa
    22561 atatccaaat tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa
    22621 acctctcccc tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg
    22681 ttcgaatggc attggagcga attgacggta tacgggacga accaatcatt ttgacttatc
    22741 ctgcacgatt gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag
    22801 gttgtgaaaa gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct
    22861 tacttatact tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc
    22921 gtgtaatcaa attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat
    22981 tgcacgggta cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat
    23041 gagtatgcat ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc
    23101 acactagctt ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac
    23161 gcaacggttt tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc
    23221 ttcaaatcaa caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc
    23281 tgtttgattt tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat
    23341 gcgatttgtg aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg
    23401 tcgtcttttt cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta
    23461 gggtattctg aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct
    23521 gaaactaatt ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca
    23581 ccatgtaccc aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca
    23641 taagtattct ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt
    23701 tctgcttgat cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg
    23761 atcaagaatg ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc
    23821 ccaagaatga ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc
    23881 cctcagtagt tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct
    23941 cctagatgat ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt
    24001 ttttccattg ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag
    24061 gacttaagca caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca
    24121 cttgctggtg ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat
    24181 ttatcttcag agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct
    24241 aaataatagg cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc
    24301 cctgatgccg ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa
    24361 tcatttgaga atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa
    24421 cgaaaaatct gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa
    24481 cagtccttat tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat
    24541 aacaaaagtt aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg
    24601 ccttttctca tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca
    24661 ccttgtttct ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt
    24721 taacgaatga agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa
    24781 ctttcttcac ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg
    24841 gattacatga gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat
    24901 gatgatctaa tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt
    24961 cttgccattt tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt
    25021 tattatttaa agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat
    25081 acaccttatc cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt
    25141 cgatcacaat gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact
    25201 ctgcttgttg agtatttact tgataaaatg actttgctga gattcgatag gtttttccta
    25261 atagctgatc ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca
    25321 catttgtttt ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat
    25381 gaatagcttc agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga
    25441 ccatcatttc atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat
    25501 ttttttcttc attatatcct cgtacttgga accgttgcaa gatatctctg actactttga
    25561 ttgcttgatc gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc
    25621 tattcttttt gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg
    25681 ctttattacg gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg
    25741 gcatcttagc aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt
    25801 gttcatactt caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta
    25861 caggctgacg atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat
    25921 tgcctgcttt tactactagt atttctactt cttctccagg gagtgcattt tcgataaata
    25981 atggataacc atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga
    26041 ctgtatatcg ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc
    26101 ctatttttca atcctactga actctactat aaatcagcta attacacaac taaaaccggc
    26161 actgacctta ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc
    26221 tgctattttt ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa
    26281 ttctttgctg acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat
    26341 ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc
    26401 ttccccgccg tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt
    26461 ttcaacgaac aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc
    26521 gccattcagc atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat
    26581 gattaaagaa aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc
    26641 aacagagttc gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc
    26701 atacttcaag cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag
    26761 ataagcaaga taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt
    26821 caaatagcca cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat
    26881 ttttacggtt tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat
    26941 ttttaatgct cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg
    27001 gtgttcaagc ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc
    27061 cactactaga tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg
    27121 ttctggtgta gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat
    27181 atccgccaaa ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc
    27241 ttttttcatt ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac
    27301 accaggattt gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc
    27361 acgatctttt ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt
    27421 aatgattggc agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac
    27481 ttcttgggct tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga
    27541 gctgatcgtt ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt
    27601 tgtctcagct aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca
    27661 gttagatgct tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat
    27721 ttcttttgta agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata
    27781 gcgttcgcga cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc
    27841 gtcatcgatc acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc
    27901 ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg
    27961 ttggatttct cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc
    28021 ttttttcacg aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc
    28081 ttgtccataa gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt
    28141 cacatcgctg acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc
    28201 ttcttctggt gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg
    28261 gttcaaatca acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc
    28321 taaatggaca cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca
    28381 tccgtcatga ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg
    28441 gtaaaagtaa tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa
    28501 agctgctttc atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata
    28561 accccagtcg atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg
    28621 agagaaaatt tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc
    28681 aaaattcatt cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct
    28741 tgttcaaacg catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc
    28801 tgcaatccaa ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca
    28861 gccaagttga cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg
    28921 ttctcaccga ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct
    28981 gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag
    29041 taagcgtcat aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact
    29101 tcttcaccga atccttctga acgtgagttg acatatacat catctaatga tttcacattt
    29161 tcagagcgat aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat
    29221 gaggcaatga tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc
    29281 acaacagcac ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca
    29341 ggggccacac cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg
    29401 cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg
    29461 tcataaccgc tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt
    29521 ccgatttggt caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa
    29581 gtaggtttca tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta
    29641 tcactaccta aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa
    29701 gagccaccag gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt
    29761 tctgtacttc cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct
    29821 gcttgataga ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc
    29881 ttagatgcag cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga
    29941 ataccagcaa ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc
    30001 aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt
    30061 cgttcaaatg ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga
    30121 tgcagttctt ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat
    30181 cgatgattgc gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt
    30241 catcgcggtt ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca
    30301 cattcgacgt aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga
    30361 tgattttacc taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg
    30421 ctaatttggc aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta
    30481 aacgagttaa aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta
    30541 ttgaaatttg tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg
    30601 catctgccat ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct
    30661 tgctgccagc atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca
    30721 ctttcccgcc aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt
    30781 taccagtaag gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat
    30841 ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat
    30901 ggacttcttc gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat
    30961 caagcgcaat aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt
    31021 ctgctaaaat ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc
    31081 gctcgaccga attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt
    31141 cttttatttt gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat
    31201 ctgctactag ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat
    31261 tcatcgcatt tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag
    31321 gattgatgca ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg
    31381 gacaatgtgt aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt
    31441 gcgaaacttc tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga
    31501 tatccttcaa cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg
    31561 ctactcggac tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc
    31621 attcgatgtt ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc
    31681 atctcggtgc tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga
    31741 tgacgattcc atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt
    31801 atgaccacac ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa
    31861 ttttttctag ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag
    31921 ctactgtgta taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac
    31981 gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc
    32041 tgtcttggtt caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga
    32101 tgttcattgg ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga
    32161 tgttttctcc tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat
    32221 aacgcaaaga aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc
    32281 ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt
    32341 tcaaactgta aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc
    32401 ctactcgttg agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta
    32461 ctaattctgc gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt
    32521 agtattcgcg agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt
    32581 cgtcaatcgt catttcatgt tccatcattt accctccact actaagcttt ttcaattggc
    32641 gcaaaggcag ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga
    32701 tccttcgacg tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact
    32761 ttatctccag gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc
    32821 gctgtctctg gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta
    32881 ggcgtaggac gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga
    32941 gaaggtcggt tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac
    33001 aacgcttctt ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg
    33061 ctttcttcca tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat
    33121 acaactggaa attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca
    33181 tcttcttcta aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt
    33241 ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct
    33301 tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt
    33361 tggattttta agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc
    33421 actgtcaagt atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat
    33481 tgctgtcctg cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt
    33541 ggccattcat gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct
    33601 cgtttgggcg aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca
    33661 ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc
    33721 attttataag gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc
    33781 gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc
    33841 atccggtcta cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc
    33901 ttgtttcctt ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca
    33961 atgacttggt ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa
    34021 attactgcag catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg
    34081 cgccagccgt aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct
    34141 gccaataaat tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga
    34201 atataatgga atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga
    34261 atcgtattca tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg
    34321 taagcagcat aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa
    34381 tcctgagggg tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga
    34441 tcatattttt tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt
    34501 tcagaagaat cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt
    34561 aaaatacgca cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg
    34621 agaatcgcat tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct
    34681 aggatgttcc aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc
    34741 actctcgtct ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga
    34801 acagcttctt tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac
    34861 aacatccttt cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta
    34921 gctagttcaa aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg
    34981 cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta
    35041 gataagactc agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc
    35101 gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat
    35161 gaaatgagtc gggaacagcc gatttactta attggcctag ctatttgctg catccttgca
    35221 gcatttttcg tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca
    35281 caagtagagg gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa
    35341 aagttcattg gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct
    35401 tctatacagc ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct
    35461 tcagaagaaa agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat
    35521 gctcctatcg caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt
    35581 gtttggctga catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc
    35641 ggcttgagac ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt
    35701 tccttagtga tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg
    35761 ctggctttgc caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt
    35821 cctttcttgc tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat
    35881 caaatcgttt tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt
    35941 gttcttcgat tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc
    36001 cttccgatat tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat
    36061 ttcgggatgg atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc
    36121 acagcgattg gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct
    36181 cttaatcatc tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt
    36241 ctcggcggta atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc
    36301 gcctcactaa aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta
    36361 gacggattaa tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc
    36421 agaggaaatc aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg
    36481 gttatcttga ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct
    36541 tctatcacaa atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact
    36601 attcatctac actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc
    36661 cagcctcttc ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca
    36721 taaatcacac cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga
    36781 ctcagtaaga atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct
    36841 ccgactaatc cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg
    36901 tcagctgctg caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc
    36961 gtcaaaccag catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg
    37021 aaaattgcca atggaggtac cattcctcca gccataacag aagccatcac gacacttccg
    37081 ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt
    37141 ggaccgccta aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc
    37201 gaaccactca tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca
    37261 ttgaccagca acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc
    37321 gttttaattc catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg
    37381 acataaccag ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta
    37441 gctaatgcac cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca
    37501 ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac
    37561 cagctagcag cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata
    37621 aattggtcga tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac
    37681 atgtgtgata cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca
    37741 tttccttctt ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta
    37801 agggctagat tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc
    37861 agttcttttc cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct
    37921 tctgcacggg cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt
    37981 tcgactttga tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg
    38041 taagtgtgag cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga
    38101 tgagcagaag cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc
    38161 tcttcagctg tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt
    38221 cctacgaaat ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg
    38281 tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag
    38341 gcttcataat ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca
    38401 ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg
    38461 cgggctagaa ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt
    38521 ttttggacca tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc
    38581 gcttctttga ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt
    38641 ttgtgatttc aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag
    38701 aaaaagctgt tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg
    38761 tacggctgta agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt
    38821 tcaatgggcg aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac
    38881 cgtctccagc cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat
    38941 acggaatgat ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat
    39001 tgtttggttt gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg
    39061 tatcaatcac gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc
    39121 cttttcgcag gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg
    39181 ttcctaatgc ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat
    39241 tgatctcagt ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg
    39301 ttgtgaacaa agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga
    39361 accctcctaa aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa
    39421 cattgatccc ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta
    39481 gttttaaccc atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa
    39541 tcattgtatt gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg
    39601 acaacgctcg gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg
    39661 gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg
    39721 tttaacagct gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt
    39781 cattcccata aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc
    39841 taaaacagca ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa
    39901 atcaactaaa tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg
    39961 cagaaaagga atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc
    40021 attaatggtt tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga
    40081 tttttcactc atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac
    40141 tctttctaga aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga
    40201 agcattcaaa aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc
    40261 taggattttt tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat
    40321 agcatatttt ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa
    40381 tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc
    40441 atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca
    40501 ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa
    40561 aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat
    40621 gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt
    40681 ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg
    40741 ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt
    40801 aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg
    40861 aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc
    40921 tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg
    40981 atcgataact ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc
    41041 ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata
    41101 actttttgtt acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg
    41161 atactggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa
    41221 taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga
    41281 ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca
    41341 tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg
    41401 tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca
    41461 taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc
    41521 gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg
    41581 ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa
    41641 caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt
    41701 gaaaacagag atagacaata cattttttat caataattga ttatcttcca agcgaaaact
    41761 acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat
    41821 tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat
    41881 aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt
    41941 ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat
    42001 tttctcacct ctttcaaatc tttttataat ttaattataa ccaa
//
LOCUS       DACDID010000009        37824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDID010000009 DACDID010000000
VERSION     DACDID010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.009"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..328)
                     /locus_tag="IV236_000368"
     CDS             complement(<1..328)
                     /locus_tag="IV236_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8770592.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            505..1233
                     /gene="sfsA"
                     /locus_tag="IV236_000369"
     CDS             505..1233
                     /gene="sfsA"
                     /locus_tag="IV236_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8770593.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1249..2262
                     /locus_tag="IV236_000370"
     CDS             1249..2262
                     /locus_tag="IV236_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8770594.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2262..3539
                     /locus_tag="IV236_000371"
     CDS             2262..3539
                     /locus_tag="IV236_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8770595.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3602..6304
                     /locus_tag="IV236_000372"
     CDS             3602..6304
                     /locus_tag="IV236_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8770596.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6456..6773
                     /locus_tag="IV236_000373"
     CDS             6456..6773
                     /locus_tag="IV236_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8770597.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6803..7123
                     /locus_tag="IV236_000374"
     CDS             6803..7123
                     /locus_tag="IV236_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8770598.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7231..8691
                     /locus_tag="IV236_000375"
     CDS             7231..8691
                     /locus_tag="IV236_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8770599.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8759..8980
                     /locus_tag="IV236_000376"
     CDS             8759..8980
                     /locus_tag="IV236_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770600.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9011..9193
                     /locus_tag="IV236_000377"
     CDS             9011..9193
                     /locus_tag="IV236_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8770601.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9193..9606
                     /locus_tag="IV236_000378"
     CDS             9193..9606
                     /locus_tag="IV236_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8770602.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9729..10910
                     /locus_tag="IV236_000379"
     CDS             9729..10910
                     /locus_tag="IV236_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770603.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            11299..11372
                     /locus_tag="IV236_000380"
     tRNA            11299..11372
                     /locus_tag="IV236_000380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11333..11335,aa:Arg,seq:cct)
     gene            complement(11409..11609)
                     /locus_tag="IV236_000381"
     CDS             complement(11409..11609)
                     /locus_tag="IV236_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8770604.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(11914..13146)
                     /locus_tag="IV236_000382"
     CDS             complement(11914..13146)
                     /locus_tag="IV236_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8770605.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            13400..13969
                     /locus_tag="IV236_000383"
     CDS             13400..13969
                     /locus_tag="IV236_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8770606.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(14147..14587)
                     /locus_tag="IV236_000384"
     CDS             complement(14147..14587)
                     /locus_tag="IV236_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8770607.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            14745..15509
                     /gene="map"
                     /locus_tag="IV236_000385"
     CDS             14745..15509
                     /gene="map"
                     /locus_tag="IV236_000385"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8770608.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            15541..16464
                     /locus_tag="IV236_000386"
     CDS             15541..16464
                     /locus_tag="IV236_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8770609.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            16540..17679
                     /locus_tag="IV236_000387"
     CDS             16540..17679
                     /locus_tag="IV236_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8770610.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            17672..18472
                     /locus_tag="IV236_000388"
     CDS             17672..18472
                     /locus_tag="IV236_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8770611.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            18472..19299
                     /locus_tag="IV236_000389"
     CDS             18472..19299
                     /locus_tag="IV236_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8770612.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            19277..20011
                     /locus_tag="IV236_000390"
     CDS             19277..20011
                     /locus_tag="IV236_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8770613.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            20111..20977
                     /gene="rfbA"
                     /locus_tag="IV236_000391"
     CDS             20111..20977
                     /gene="rfbA"
                     /locus_tag="IV236_000391"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8770614.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            20991..21563
                     /gene="rfbC"
                     /locus_tag="IV236_000392"
     CDS             20991..21563
                     /gene="rfbC"
                     /locus_tag="IV236_000392"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8770615.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            21585..22613
                     /gene="rfbB"
                     /locus_tag="IV236_000393"
     CDS             21585..22613
                     /gene="rfbB"
                     /locus_tag="IV236_000393"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8770616.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            22711..23562
                     /gene="rfbD"
                     /locus_tag="IV236_000394"
     CDS             22711..23562
                     /gene="rfbD"
                     /locus_tag="IV236_000394"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8770617.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            23597..25630
                     /locus_tag="IV236_000395"
     CDS             23597..25630
                     /locus_tag="IV236_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770618.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            25674..26954
                     /locus_tag="IV236_000396"
     CDS             25674..26954
                     /locus_tag="IV236_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8770619.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            27164..27970
                     /locus_tag="IV236_000397"
     CDS             27164..27970
                     /locus_tag="IV236_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770620.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            27982..29202
                     /locus_tag="IV236_000398"
     CDS             27982..29202
                     /locus_tag="IV236_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770621.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            29192..30778
                     /locus_tag="IV236_000399"
     CDS             29192..30778
                     /locus_tag="IV236_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8770622.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            30817..32955
                     /locus_tag="IV236_000400"
     CDS             30817..32955
                     /locus_tag="IV236_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8770623.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(33323..34306)
                     /locus_tag="IV236_000401"
     CDS             complement(33323..34306)
                     /locus_tag="IV236_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8770624.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            34684..36075
                     /locus_tag="IV236_000402"
     CDS             34684..36075
                     /locus_tag="IV236_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8770625.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            36091..37131
                     /locus_tag="IV236_000403"
     CDS             36091..37131
                     /locus_tag="IV236_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8770626.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            37150..>37824
                     /locus_tag="IV236_000404"
     CDS             37150..>37824
                     /locus_tag="IV236_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8770627.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNS"
BASE COUNT        12163 a         6369 c         7789 g        11503 t
ORIGIN      
        1 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
       61 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
      121 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
      181 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
      241 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
      301 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
      361 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
      421 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
      481 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
      541 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
      601 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
      661 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
      721 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
      781 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
      841 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
      901 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
      961 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
     1021 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
     1081 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
     1141 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
     1201 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
     1261 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
     1321 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
     1381 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
     1441 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
     1501 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
     1561 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
     1621 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
     1681 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
     1741 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
     1801 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
     1861 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
     1921 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
     1981 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
     2041 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
     2101 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
     2161 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
     2221 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
     2281 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
     2341 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
     2401 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
     2461 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
     2521 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
     2581 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
     2641 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
     2701 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
     2761 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
     2821 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
     2881 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
     2941 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
     3001 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
     3061 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
     3121 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
     3181 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
     3241 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
     3301 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
     3361 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
     3421 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
     3481 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
     3541 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
     3601 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
     3661 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
     3721 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
     3781 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
     3841 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
     3901 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
     3961 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
     4021 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
     4081 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
     4141 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
     4201 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
     4261 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
     4321 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
     4381 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
     4441 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
     4501 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
     4561 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
     4621 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
     4681 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
     4741 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
     4801 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
     4861 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
     4921 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
     4981 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
     5041 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
     5101 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
     5161 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
     5221 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
     5281 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
     5341 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
     5401 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
     5461 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
     5521 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
     5581 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
     5641 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
     5701 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
     5761 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
     5821 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
     5881 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
     5941 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
     6001 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
     6061 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
     6121 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
     6181 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
     6241 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
     6301 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
     6361 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
     6421 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
     6481 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
     6541 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
     6601 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
     6661 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
     6721 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
     6781 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
     6841 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
     6901 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
     6961 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
     7021 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
     7081 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
     7141 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
     7201 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
     7261 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
     7321 gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
     7381 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
     7441 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
     7501 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
     7561 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
     7621 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
     7681 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
     7741 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
     7801 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
     7861 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
     7921 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
     7981 atcattgatt tggtgcaaaa atatattgca gagccattcc taggattatc tcaagggttg
     8041 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
     8101 ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
     8161 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
     8221 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
     8281 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
     8341 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
     8401 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
     8461 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
     8521 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
     8581 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
     8641 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
     8701 gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat
     8761 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
     8821 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
     8881 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
     8941 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
     9001 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
     9061 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
     9121 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
     9181 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
     9241 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
     9301 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
     9361 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
     9421 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
     9481 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
     9541 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
     9601 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
     9661 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
     9721 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
     9781 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
     9841 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
     9901 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
     9961 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    10021 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    10081 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    10141 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    10201 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    10261 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    10321 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    10381 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    10441 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    10501 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    10561 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    10621 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    10681 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    10741 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    10801 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    10861 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    10921 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    10981 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    11041 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    11101 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    11161 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    11221 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    11281 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    11341 ataggtcgct ggttcaattc cagtcaggaa cgtacaaata acccctgtat cctttgcggt
    11401 acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    11461 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
    11521 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
    11581 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
    11641 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
    11701 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
    11761 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt
    11821 ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt
    11881 tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat ttcggaaaat
    11941 cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag atcctaccat
    12001 gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt catcggacat
    12061 ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt gattcgatgc
    12121 gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag gtgaagggtc
    12181 aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc
    12241 taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt taaaagtaaa
    12301 tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg aaatgtaacc
    12361 atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa
    12421 tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt taggcaatcc
    12481 gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt ctcgattcaa
    12541 ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga cagggtcttc
    12601 ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt gtttttcttc
    12661 tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca cgacagtcca
    12721 actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc cattcgctga
    12781 ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat cggcagaaac
    12841 aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga ttttagattg
    12901 tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt ggatctggtc
    12961 gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt gtgtgatcaa
    13021 tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt
    13081 ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat taaaatcaga
    13141 taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat gataccattt
    13201 aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt ttaaggttaa
    13261 gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca aaaactaatt
    13321 ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa
    13381 attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac agattttggc
    13441 agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga gaaacatcgc
    13501 gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata tggatgactt
    13561 gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt tcactgttga
    13621 gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg caagaagaga
    13681 gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag agatgcagaa
    13741 tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg tgaatctttc
    13801 agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg ggatcgcagc
    13861 tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga tgatccaaga
    13921 cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa tatactcaaa
    13981 agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct
    14041 tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg aataaaagag
    14101 ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa atttttgcga
    14161 ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca ttcagatcca
    14221 ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat
    14281 ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt
    14341 ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca tcaggcagat
    14401 cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg aattcatctg
    14461 catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca aatgcttctg
    14521 caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg atttttgcta
    14581 aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat accaaaacat
    14641 cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct aaatgtatgc
    14701 cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt actttaaaat
    14761 cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat gtacataaaa
    14821 atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta tttgtcagaa
    14881 actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg
    14941 cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa aagcctttga
    15001 aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc atttctgatt
    15061 cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg atggaagtaa
    15121 cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg attggtgaca
    15181 tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta
    15241 ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat tacggagaag
    15301 ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct atggttaata
    15361 ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca agagacggcg
    15421 gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca agaattttga
    15481 catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg ggagaggtac
    15541 atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat tgagacgact
    15601 caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc ttattattta
    15661 ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt tctccacatc
    15721 gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt ttaccagttt
    15781 atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt atccgtttct
    15841 gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat
    15901 aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt ctcactaatt
    15961 gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg gttgggacag
    16021 ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat cgatcagttc
    16081 caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt cttgatttat
    16141 ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac gatcttcgct
    16201 actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta ttttgcaacg
    16261 aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg gcttaatttt
    16321 gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga atacgtgacg
    16381 ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa attgaaaact
    16441 aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta tatcagtaat
    16501 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    16561 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    16621 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    16681 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    16741 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    16801 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    16861 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    16921 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    16981 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    17041 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    17101 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    17161 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    17221 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    17281 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    17341 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    17401 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    17461 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcgtatt gattattgcc
    17521 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    17581 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    17641 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    17701 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    17761 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    17821 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    17881 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    17941 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    18001 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    18061 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    18121 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    18181 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    18241 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    18301 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    18361 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    18421 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    18481 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    18541 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    18601 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    18661 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    18721 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    18781 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    18841 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    18901 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    18961 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    19021 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    19081 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    19141 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    19201 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    19261 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    19321 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    19381 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    19441 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    19501 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    19561 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    19621 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    19681 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    19741 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    19801 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    19861 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    19921 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    19981 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    20041 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    20101 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    20161 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    20221 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    20281 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    20341 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    20401 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    20461 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    20521 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    20581 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    20641 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    20701 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    20761 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    20821 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    20881 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    20941 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    21001 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    21061 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    21121 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    21181 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    21241 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    21301 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    21361 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    21421 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    21481 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    21541 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    21601 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    21661 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    21721 ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    21781 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    21841 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    21901 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    21961 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    22021 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    22081 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    22141 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgagta
    22201 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    22261 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    22321 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    22381 aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca gggcacgatt
    22441 tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag cctgagttta
    22501 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    22561 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    22621 gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    22681 taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg aaatggacaa
    22741 ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt ttcaacagat
    22801 gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac agaattaaaa
    22861 cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga agaagaagca
    22921 aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact tgcagcaaaa
    22981 gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    23041 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    23101 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    23161 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    23221 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    23281 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    23341 aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga tacagatgta
    23401 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    23461 gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg gcaagaagca
    23521 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt ttatcaaata
    23581 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    23641 agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    23701 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    23761 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    23821 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    23881 tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    23941 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    24001 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    24061 ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag ctctatcgct
    24121 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    24181 gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt atttctacaa
    24241 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    24301 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    24361 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    24421 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    24481 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    24541 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    24601 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    24661 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    24721 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    24781 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    24841 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    24901 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    24961 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    25021 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    25081 ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    25141 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    25201 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    25261 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    25321 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    25381 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    25441 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    25501 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    25561 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    25621 cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg agagtgaaga
    25681 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    25741 tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca actttgttta
    25801 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    25861 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    25921 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    25981 tggttatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    26041 tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    26101 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    26161 tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    26221 ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc gttgcagcct
    26281 ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    26341 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcctaca
    26401 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    26461 atttgtttac cgatcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    26521 ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac caatttttag
    26581 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    26641 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    26701 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    26761 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    26821 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    26881 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    26941 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    27001 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    27061 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    27121 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    27181 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    27241 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    27301 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    27361 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    27421 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    27481 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccattt
    27541 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    27601 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    27661 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    27721 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    27781 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    27841 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    27901 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    27961 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    28021 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    28081 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    28141 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    28201 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    28261 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    28321 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    28381 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    28441 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    28501 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    28561 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    28621 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    28681 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    28741 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    28801 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    28861 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    28921 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    28981 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    29041 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    29101 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    29161 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    29221 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    29281 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    29341 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    29401 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    29461 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    29521 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    29581 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    29641 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    29701 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    29761 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    29821 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    29881 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    29941 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    30001 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    30061 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    30121 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    30181 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    30241 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    30301 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    30361 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    30421 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    30481 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    30541 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    30601 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    30661 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    30721 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    30781 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    30841 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    30901 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    30961 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    31021 caaagcggtt tcaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    31081 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    31141 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    31201 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    31261 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    31321 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    31381 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    31441 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    31501 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    31561 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    31621 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    31681 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    31741 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    31801 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    31861 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    31921 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    31981 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    32041 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    32101 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    32161 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    32221 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    32281 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    32341 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    32401 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    32461 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    32521 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    32581 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    32641 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    32701 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    32761 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    32821 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    32881 attgcgcatg atccatttta caatgataat ttaacaaaag acggaatcgg cgacttttcg
    32941 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    33001 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    33061 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    33121 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    33181 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    33241 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    33301 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    33361 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    33421 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    33481 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    33541 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    33601 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    33661 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    33721 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    33781 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    33841 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    33901 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    33961 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    34021 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    34081 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    34141 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    34201 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    34261 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    34321 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    34381 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    34441 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    34501 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    34561 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    34621 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    34681 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    34741 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    34801 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    34861 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    34921 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    34981 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    35041 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    35101 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    35161 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    35221 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    35281 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    35341 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    35401 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    35461 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    35521 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    35581 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    35641 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    35701 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    35761 ggaccacgtc ctgaacgttc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    35821 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    35881 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    35941 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    36001 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    36061 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    36121 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    36181 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    36241 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    36301 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    36361 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    36421 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    36481 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    36541 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    36601 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    36661 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    36721 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    36781 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    36841 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    36901 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    36961 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    37021 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    37081 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    37141 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    37201 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    37261 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    37321 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    37381 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    37441 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    37501 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    37561 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    37621 tttcaaaacc atccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    37681 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    37741 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    37801 ttagaaatac taaaaatgaa ttct
//
LOCUS       DACDID010000010        37317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDID010000010 DACDID010000000
VERSION     DACDID010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.010"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..749
                     /locus_tag="IV236_000405"
     CDS             72..749
                     /locus_tag="IV236_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770628.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            916..1878
                     /locus_tag="IV236_000406"
     CDS             916..1878
                     /locus_tag="IV236_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770629.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2078..2518)
                     /locus_tag="IV236_000407"
     CDS             complement(2078..2518)
                     /locus_tag="IV236_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8770630.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2529..4562)
                     /locus_tag="IV236_000408"
     CDS             complement(2529..4562)
                     /locus_tag="IV236_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8770631.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4564..6267)
                     /locus_tag="IV236_000409"
     CDS             complement(4564..6267)
                     /locus_tag="IV236_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770632.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6361..7311)
                     /gene="manA"
                     /locus_tag="IV236_000410"
     CDS             complement(6361..7311)
                     /gene="manA"
                     /locus_tag="IV236_000410"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8770633.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7350..8375)
                     /locus_tag="IV236_000411"
     CDS             complement(7350..8375)
                     /locus_tag="IV236_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8770634.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8584..9453)
                     /locus_tag="IV236_000412"
     CDS             complement(8584..9453)
                     /locus_tag="IV236_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8770635.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9912..11804)
                     /locus_tag="IV236_000413"
     CDS             complement(9912..11804)
                     /locus_tag="IV236_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770636.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11801..13528)
                     /locus_tag="IV236_000414"
     CDS             complement(11801..13528)
                     /locus_tag="IV236_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770637.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13544..14194)
                     /locus_tag="IV236_000415"
     CDS             complement(13544..14194)
                     /locus_tag="IV236_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8770638.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14398..14694
                     /locus_tag="IV236_000416"
     CDS             14398..14694
                     /locus_tag="IV236_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8770639.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14866..15486
                     /locus_tag="IV236_000417"
     CDS             14866..15486
                     /locus_tag="IV236_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8770640.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15578..16744)
                     /locus_tag="IV236_000418"
     CDS             complement(15578..16744)
                     /locus_tag="IV236_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8770641.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16756..17109)
                     /locus_tag="IV236_000419"
     CDS             complement(16756..17109)
                     /locus_tag="IV236_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8770642.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17237..18259
                     /locus_tag="IV236_000420"
     CDS             17237..18259
                     /locus_tag="IV236_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8770643.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18698..19132)
                     /locus_tag="IV236_000421"
     CDS             complement(18698..19132)
                     /locus_tag="IV236_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770644.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19242..21101)
                     /locus_tag="IV236_000422"
     CDS             complement(19242..21101)
                     /locus_tag="IV236_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8770645.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21219..22379)
                     /locus_tag="IV236_000423"
     CDS             complement(21219..22379)
                     /locus_tag="IV236_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8770646.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22686..24110)
                     /gene="araA"
                     /locus_tag="IV236_000424"
     CDS             complement(22686..24110)
                     /gene="araA"
                     /locus_tag="IV236_000424"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8770647.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24126..24824)
                     /locus_tag="IV236_000425"
     CDS             complement(24126..24824)
                     /locus_tag="IV236_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8770648.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24781..26442)
                     /locus_tag="IV236_000426"
     CDS             complement(24781..26442)
                     /locus_tag="IV236_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8770649.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26660..28060
                     /locus_tag="IV236_000427"
     CDS             26660..28060
                     /locus_tag="IV236_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8770650.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28312..29391)
                     /locus_tag="IV236_000428"
     CDS             complement(28312..29391)
                     /locus_tag="IV236_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8770651.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29477..30436)
                     /locus_tag="IV236_000429"
     CDS             complement(29477..30436)
                     /locus_tag="IV236_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8770652.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30448..31971)
                     /locus_tag="IV236_000430"
     CDS             complement(30448..31971)
                     /locus_tag="IV236_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8770653.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31981..32544)
                     /locus_tag="IV236_000431"
     CDS             complement(31981..32544)
                     /locus_tag="IV236_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8770654.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32550..33413)
                     /locus_tag="IV236_000432"
     CDS             complement(32550..33413)
                     /locus_tag="IV236_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8770655.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33427..34323)
                     /locus_tag="IV236_000433"
     CDS             complement(33427..34323)
                     /locus_tag="IV236_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8770656.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34498..35769)
                     /locus_tag="IV236_000434"
     CDS             complement(34498..35769)
                     /locus_tag="IV236_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8770657.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35759..37276)
                     /locus_tag="IV236_000435"
     CDS             complement(35759..37276)
                     /locus_tag="IV236_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8770658.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10807 a         7808 c         6453 g        12249 t
ORIGIN      
        1 cttttgttag catttaattt agatgttttc cattctatta tgttattaaa ttttatattg
       61 gaggcctatc tatgacatta ctgatcaaaa acatgaacat gaaacgtggt aaaaaacgtg
      121 tgattcgaca attgaatctt ttagtagaat caggagaagt agctgctttg attgccccga
      181 acggatttgg caaaacaact ttgctgaatg gcattgccca actgctgcca acaacttatg
      241 atcaattgtc ccttcacaac atctctccta ctgcttatac agattatcgc cggtctttct
      301 tttttctaga atcaacgaat cagttatccg actatctatc ggctgttgat tatttgactt
      361 tgatcaaata ctattggcag agcaaagtag aatgccaaac tgttttccaa cgattgaaga
      421 tgactgagta ccttactttg ccgattggaa aaatgtctct aggaatgaaa caacatgtct
      481 tgcttgctgc ttacttgatc agtgattcat ctgtcatgtt attcgatgaa ccgttgaacg
      541 gattagaccc aggaagtatt gatatcttga atagcctcat cagacaatgg cagaaagagg
      601 ggaagacgat tcttctttct tcccacgcta tcgcgaatat ccaatcgatt tgttctaaag
      661 catgttttct aaaaaatgga gaaatcgtca cagaatcctc aaatatggaa gagattttag
      721 ctatttatcg taaattatat gaggtgtaac tatgtcttta cttacatttg aacgcaatct
      781 cttactaaaa aacaaagtaa actttttatt tcctctattg ttggtcgtgc tctttgcatt
      841 cccacttttt ttgatcataa acttgcttat acagaattcg atgaactgaa tcataactat
      901 gaagagatgc aacgtttgat tgaaacgttg aaagaagatg agaatgagaa agaatttgtg
      961 gaaagtttag agaaaagcaa caaactgatc gaagcaatct tacatgcaaa aaacacaggg
     1021 aatgtccagc aaatggtcga agcaacttat cattatgaaa aagatatact ggatcgactg
     1081 atttctggtc aaagacaagg aattccaatc attgaacaac aaaaacgtgt cgagttgctc
     1141 cgttatatga aagaacatca gatccaaaga tattctattt ttgacttgcc agcgcatctc
     1201 cctttagcaa attactacga aaatattttt tcaggaatga tttctagttt tttgatttta
     1261 tgtataaccg ccttgtttct ttcttccatc atctcttatg aaaaaagaaa gcaggtaatc
     1321 tctctagtca atctattacc ggatagtatg gtaaagaaac atagcatccg ttttaccatt
     1381 tattatggtg cagctatgct aagtctcgtg atgccatttt tgatcgtctc tatactagta
     1441 ataatcaaaa atggtttggg agacttccgt tatcctgtag gaacgatcat tggtcaggag
     1501 atacgaatcc ttccgatgta tgagtatctt ttccagagct ttctgttcct gttgttgtgg
     1561 gtgctctttt taagtacaat cagttttctt ctatctgctt tatttgaaca ttctctagtc
     1621 aatcttttag gtacacttct ttgtcttttc ttagcagagt atcgcttgtt ttcttcgatt
     1681 ggatggatag aatcaatcag tcattacttg ccaacttctt atgttgattt tcaaaatgtg
     1741 atcataggag gagatatttt ctcgccacta gcttcagaac aggttacttt catgaatggt
     1801 atcttgacac ttggtatatg gagtatcgtt ttattattca taggtatggg cactatctat
     1861 ataaaaaaga gctattaaaa cacaaaaata ataggggcgg tgacaaaaac cttgtcaccg
     1921 tcccctattt caaataaacg gttttctaga ataaatagtt ttcaaaagct tgcatccgca
     1981 gtaataagaa cctcccaaca aaaatagctc ctcttatttt tatcttggtt gaagttatta
     2041 cttgatgcaa ggcagctttt tttactgtga acattggtta tatatgtcca tgttcaaaaa
     2101 tcatttggac ttcttcaatg gttttcgcat cactgagcgc cattacttgg tcgatgtctg
     2161 aacagaaaaa ggctaaatct tggagcaccg ctaactgatg ttcttttttt aaagccacta
     2221 caaataagag tgtcgcgatt tttggttcct cttttgttcc aaagtccaca ccatcaggca
     2281 cttggactaa atagattcct tcttctacta cttcttcatc tttgtcagtc ccatgtggca
     2341 aggctacgaa attcccgata tagacactcg ctttttcttg tctttcgatc attgattgga
     2401 tatatgccgg tttgattccc ccttgttggg tcatccactg gccgatctcg gtcaacgctt
     2461 ctttccaatt ttgataggat tgatcgagca atattttttc tgctggtagc tgtatcatct
     2521 tttcaccttc atttctttat ttcttcaatc aggattttcg ctaacaactg ataaacgatt
     2581 tctttatttc ctgagcggaa gatctcggtg ttcaagtcat tcatgatgat tgctccactg
     2641 atctttccta aaagaatccc gtctttttct tggattggcg aaggcgccag cataactaac
     2701 atacgggaca aatcgactgg ctgtctatcc atcccttcaa tggtcaacgg atgtttcaaa
     2761 ttgaaaatac aaaagatcgg tttagaaatc aatgagctgg atgtatggaa taaccccata
     2821 tttgtatgag gaataccgat aggtgcttgt tcatatcgct taagcagccg gcgatgaacc
     2881 acctcttcat ccgttactag tccttcagga aaataaggaa ataagctgcg aatcgtttcg
     2941 gaaatcgtct caggattatc cagttcttgg ataaaaaaac gaccgagtaa ttgattgatt
     3001 tgatccatga aatgcattgt ttcctcataa gtatcttcta tcatccactc gttattgctg
     3061 ttatacgtaa ttttttgcct ttttttcttc tcaatatgcg aaaaagcttc ttttagctgc
     3121 ttgacttcat cttctagcag tagcggggag actaaaatat attttccacc gtatcctggt
     3181 aaaatagatg tagaaataac aacatcatat tcttcttgta aatcgatttt ttttaaatca
     3241 gcaatccgat agaaatgaat ttgttggatg aagggaaaat accttttcaa tcgtagttcc
     3301 aacatgctag tagaagctaa tccacttggc gaaaatcctg caatactgat attgctttct
     3361 ttcggacttc gttccaatga attcgcaaaa tgaaggacca tataagcgat ctcttcttcg
     3421 gataattttt tttcttgaaa aatccgctct actgctttac ggattgcatt cgcaacttcc
     3481 tcgtattgaa ccatgatttt ttctaaaatc ggattggtca gtgtctcttc ttgtaaaacg
     3541 gctcttgaaa acacaccaga taaatgagtc aacagcattt tgtacaaagt ttcatcttca
     3601 taaaaatgga cattcgtctc ttcacttact tcttgaatca gttgttttac taaataagca
     3661 agctgtgtat cgaacgtctc ttcaaagaaa ttctgatcaa atgagttcgt gaagtcattt
     3721 aataatcctg caaaaaagac gatctcattt actggataaa gttgttttgt ctcagaagcg
     3781 atccgtgaaa acaatcgttt agctgtctgt agtaattctt tgtttgccag actattatat
     3841 gtctcatttg tcagtaaatg atttttagtc acacgctcca tcgtcaatgt cagcatcaaa
     3901 accaaatgtt caagctttct atcacttaga ttggtctttt tttggatcaa ttcttccccg
     3961 attactttgc gtgcaagaga tagatgttgc ggatcaacga atcgaaaaaa ataattcagt
     4021 gatgcatcga aagaaatact atgaaaaagt tcatattcat tgatttctaa ttctaataca
     4081 ttcgctgtca gaagccggcg gtatttttcc ggtccttcga tctcgtatcc ctggttccga
     4141 atgatttcca aaggcagttg gcgaatactt tcttccaact gtttgatatc tgcataaaaa
     4201 gtggtattac tgatgcgata tgattccatg aaatgagcaa gactcaacgg ctctgtcgtc
     4261 agtaacaact cggctaaaat cgcgtgttgt cgtttagcag tggataagtc attacttttc
     4321 ccatctgtat tttcttttaa ttgctctaat acttcttctg atgcctcaat gacaaatgag
     4381 cctctagctg cttttttcaa agaagcattt accgatttca atgagacttc aagattatct
     4441 aattcgcggt aaatagtccg tttactgacc ttcaataaat tcatcatttt ttggatagat
     4501 acaggtgacg ggctactgag aaattctgct aataattgtt tttctctggg cgataaatac
     4561 atcttatatt tctcccttca cgagtttttc gtattcattc gtagctaccc aatgttcaac
     4621 tagaactagc cgagggatct tttcttctag ctgttcagtt acagctagtt ctctttgtgc
     4681 gatataaatc gtttcttctt ggattttgat tttttctatt gattcttttg agataacaaa
     4741 ctgttttttc gctttttttg ctaattgact taaaatcgtc atgcccatag tttgtgagcc
     4801 gcgtctgttg tttttataca aaaatacaat attagcaggc gacttttttt gttctgtgat
     4861 atctgctatc tctttttttt cttcccgctt tggaaattgg atcgcttctt ttattcgtga
     4921 cagcaagata tctgtttcca aaaagtgatc cagtacatag atttgacttt gaggtacttt
     4981 ttctcttgca agacccgcta gttcatgttg cacgataacc agctgattta cttggttatt
     5041 caattcatag atagagctgt atccgacaga aagatcgatt ttttctgctg ctaatttctt
     5101 tttcagcagg gcggctccca tagcacttga tcccatacct gagtcgcaag caaaaatgac
     5161 ggaatgaatg atagatggtt ccaagatttc tcccctttct ttgaccgttt tgttctcttt
     5221 ttctttattt ggtaatttca atagtatcgc tgcacaaatg aaggtcacaa atgtactgat
     5281 tgctatccct atacttaccc cagctagttg tggataaggc gcattggcaa aaatcacgag
     5341 tagtgaccca ggcgatacag gtgcagctaa tcccgtatct aaccattgaa atatcaacgt
     5401 gccgcttgcc ccaccagcca ttacagctaa aaatagatac ggtcgcagca agacaaatgg
     5461 aaaatagatt tcgtgaatcc cgccaatcgc atggatcatc aatgctccac tcgcatttac
     5521 tttttcctgt ttttctgcaa aacaaaggat agaaagtaag atccctaaac caggtccagg
     5581 atttgcctcc atcaaaaata aggaagagta cccgactgtt tgtgagaatt cgattcctaa
     5641 aggtgtcaat acaccatgat tgattgcatt attgaaaaat agtactttta gaggttctaa
     5701 gatcagactg acaagtggta acagattcat tgtgactagc caagacacaa cggaggcgag
     5761 caacaagtga acactattta aaactggttc aataaaaacg attccaatta tacaaataac
     5821 gctaccagtc agtcctacta gtaggttgct gacaagcatt tcatatccag ccttgaattt
     5881 cacgtggaat ctagaaacaa attgttcata tacgatgccg gcaagcggcc cgataatcat
     5941 cgctcctacg atttgtggag cattggaacg agcgatcaac cctaaagaag caattgcacc
     6001 ggcgactgcc cctttttgtc cccctaccga tttcccgcct gaataactga tcaagatggg
     6061 taaaagcagc tggatcatcc aatggcatac ctcttctaat gtggtgtgca aatcgccaga
     6121 cacacctttt gttgctaatg acaagattcc ccaagcgata aacagactta gatttggcat
     6181 gatcatttca tttaagtgat gtccagcttt tctcagttgt ttcatagaca cgcgactgct
     6241 gttgtttgct ttccatttgt attccatctt tttcaccata cttctattta ttgatggacg
     6301 gattttatgt agaaaaagca gcaaacgaaa aaccgtctgc tgctttcagc tatctgggaa
     6361 ctatttgcct ggttcagtcg caatgattgc tagttcacct tggaccgccc actcagaaac
     6421 gccatttgga ataatacagc ttgttccttt ttctaatgga tatgtttttc cgtcaacgac
     6481 aaggtctcct gctccttcga ttaccgtcat caatgtataa ggggcttgtt ttttgaaaga
     6541 tgtgattcct ttaatatccc actcatatac attgaaaaac tcagtttcta aataagtgac
     6601 gatcgatgaa ttcccttttc ggacttcttt gatttgtaat tctggtgttt tcgcagggac
     6661 agttgttaca tcgatcgatt gttggatatg cagctctctt gtttgtcctt gcgcatcttt
     6721 tctgtcgtag tcatacacgc ggtaagtcgt gtcagaactt tgctgtgttt ctaaaatcat
     6781 gattccttta ccaatagcat ggatcgttcc gcttggtaca taaaagaaat cacctttttt
     6841 aactggtact ttcttcaaaa ggtcatccca gcgcccatct ttgatcatct cagctagttc
     6901 ttctcttgtc tttgcatgat gaccatagat gatttctgca ccaggttcag catcgatgat
     6961 ataccagcat tcagtttttc ctaattcacc ttcatgtttc atcccatagg catcatctgg
     7021 atgtacttgt acagatagat catcttctgc gtctaagatc ttgatcaata atggaaacac
     7081 tggttctgaa ggatggccaa acaattcctt gtgttctgac catagttcat ctaatctttt
     7141 ccctttgaat tcgccatttt ctactacact gacaccatga ggatgcgcac tgatcgccca
     7201 atcttctccg attttatcgt tagggatatc aaaaccaaag actgaacgta aacgacttcc
     7261 tccccaaatt ttttcttgaa aaactggttt taaaaacata ggttcctgca taattcaaac
     7321 tcctctcatt tttcatagct attatagcat tatttcggta ataccgcttt ctctttcaaa
     7381 aagttttcaa caatttcatc tcgacatatg agatactccg cacgatgatc gacaggatgt
     7441 acgcgatttg acaaaaatat caacgcttct tgtgttttta tatcaatcaa gaaaaacgta
     7501 cctgtataac cagtatgaaa aagaatcgca tgaccgtctt ctacatcctt ttttaagtcc
     7561 catcctaaag aacgatagcc attttttttc ggtgtttgat cttgcagtaa agaatcaatc
     7621 gtctcttctt ctaaaaaacg accattacgg atttttccct tgttcaaata catttggaca
     7681 aaagtaatgc tgtctttcaa atcaataaag agacccgcgt tacctgcatg ttcagccaaa
     7741 actcttgctt tcggatcatg agtttgtcca cgcaaaacct cacccgaagg cagttgttct
     7801 gtgggaacga tttttttgga aggaaaagga agaaataagc tgttcatcat tcctaatggt
     7861 tccaatactt gttcttgaaa gatttccact gcagtttctt ggtaaatttc ctctagcatc
     7921 aacccaagaa gaatcgtacc ggcatctgta tattttactt cttttcctag ttgctcacct
     7981 ggttctagct tcaaataagc ggcgatcaat tcttcctgag atagcttatc tctgtttggt
     8041 atccatgttt tgatatcaga agtatgtgtc agtaaatgac gcaaagtgat tgtctgattt
     8101 tcaaattcag gaagatattt atgaagcggt tgatcgagtt ctatctttcc atcttctaat
     8161 agacgtaaaa ctaatgaagt ggttgcaacg actttcgtca gtgaggctgc atcaaacaac
     8221 agatcttctg tcatcagctg cttttcagga acgacttggg caaagccggc tgtaaatgtt
     8281 tctacttgat tttcttttat gaaggcgact gctacacctg gataaaatcc ttcttccatc
     8341 tttttcaaaa taagctgttt cgtgtgtgga tacatctttt tctcctgctt ttctatcaga
     8401 ttcttacagt ccaagcttaa agcttaatct atgaggaatc aacctttttt acacaaaaaa
     8461 actgcgcgaa aaaccgcgca gtttcccccg gtatactatt ttctctttct atctctagaa
     8521 taagcgatag tatgaccgtt cataaaatga cgagacatgc gacaatcgtt aagaaagaaa
     8581 aagttaagcg tcgcatcgaa tgtcagtttt attcttttct tttggctcac tgacgaacca
     8641 ccattcgatt cctatagcat catagattgt gagtactgtg tgctttttgt ctaagaagaa
     8701 atcaactgat agttgctgca agtgagcaat cagtccttcc atgctttcag tatcgatcat
     8761 gaatttcaga aaatcaagtc caatcacttc atctgtaggc aaatcaattg cgcctccgct
     8821 agcttctgtc aaaccaacat ggaaatctcc ttcattcaat acatggatgc cctcttcttc
     8881 attatgtacc ttcaaaccaa gtacatgatt aagaaattgg cgttcttttg ttaagttagt
     8941 aacatttaaa tggatttttt caaaataaga acctgttgat agtttactga atttttcttc
     9001 agattttctt aataatgctt cttgatccat tgtttctgga atttctcttt ggtcgttctt
     9061 attctcttca tggtaaattt ccaattcatt ctcttctggg tctacgagca aaatccccat
     9121 acgtccatga tcttctaaag cttctttgat tggataattc attttacgga tacgacaaag
     9181 gatatctgcc atttcttctg tgctagggat aatcaatgaa aaacggttca gctttttgat
     9241 cacccccatg tgatcattcg ctctaggact ttcttccagc agcaacattt cacttccagc
     9301 ttcttctgtt cctaaaatgg ctaattcgtt ttcttctctt ttcaaatcaa agccaacaac
     9361 gttcttgtaa aaatcaatca ttttatctcg gtctttcacc cgaatggcta cactttttaa
     9421 ataggtggca gcccccaatt gaaaatcttt catatttctt gctcccctct acgtcaagct
     9481 ttattttaac gatggattct tcttctttca agcaataact ttgattttgt taaaaaaaat
     9541 gtgagaaaat tcgcattttg cttgtttttt gtaaattaat gagtaaaaac tcttataaaa
     9601 agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     9661 ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     9721 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat
     9781 agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt
     9841 ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa
     9901 tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag
     9961 aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc
    10021 atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt
    10081 gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct
    10141 acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca
    10201 attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg
    10261 ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca
    10321 atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct
    10381 gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa
    10441 agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt
    10501 tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc
    10561 tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg
    10621 aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat
    10681 ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact
    10741 gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt
    10801 gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact
    10861 ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg
    10921 atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg attttctttt
    10981 tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca
    11041 tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac
    11101 acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac
    11161 gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg
    11221 actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata
    11281 tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt
    11341 acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca
    11401 cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt
    11461 actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg
    11521 ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc
    11581 cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca
    11641 gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag
    11701 aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga
    11761 ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc
    11821 ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg
    11881 ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg
    11941 tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt
    12001 ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga
    12061 tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga
    12121 agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat
    12181 ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac
    12241 catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc
    12301 ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga
    12361 tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg
    12421 aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt
    12481 ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg
    12541 cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt
    12601 catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa
    12661 tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca
    12721 gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta
    12781 cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa
    12841 ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc
    12901 gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt
    12961 ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc
    13021 cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat
    13081 ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac
    13141 gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca
    13201 aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga
    13261 catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg
    13321 cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa
    13381 tataatcgac atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca
    13441 aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca
    13501 aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt
    13561 ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt
    13621 cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta
    13681 attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca
    13741 attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt
    13801 ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt
    13861 tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg
    13921 attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag
    13981 agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat
    14041 actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt
    14101 cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt
    14161 ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat cctcctttct ttcaagatga
    14221 cacattgtca tatgacgctg tgtcattata acacctccta cctgttttgc aagacatttg
    14281 catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt
    14341 tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg
    14401 gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt
    14461 ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc
    14521 cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt
    14581 ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt
    14641 cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg
    14701 cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg
    14761 tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt
    14821 ctcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta
    14881 gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct
    14941 tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat
    15001 cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct
    15061 ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa
    15121 ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta
    15181 aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc
    15241 cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa
    15301 tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta
    15361 aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct
    15421 gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca
    15481 ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca
    15541 tttgtacctt ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg
    15601 gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta
    15661 ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc
    15721 cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta
    15781 ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt
    15841 tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa
    15901 atgatcctaa gtaagggatc aggttcaaaa aacccgcaat aatccccaac gtgacagcat
    15961 actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag
    16021 ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg
    16081 tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt
    16141 ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta
    16201 ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat
    16261 cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga
    16321 acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga
    16381 agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc
    16441 atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt
    16501 ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa
    16561 tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt
    16621 ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt
    16681 gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt
    16741 ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct
    16801 atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat
    16861 caatgatacg tgagctactg tctctaaatt cccattggcc atcggaccga cataatcgat
    16921 cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc
    16981 ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa
    17041 caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc
    17101 ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc
    17161 tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta
    17221 ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag
    17281 taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt
    17341 agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac
    17401 agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt
    17461 gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca
    17521 attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga
    17581 tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa
    17641 ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg
    17701 tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct
    17761 tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa
    17821 tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa
    17881 taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga
    17941 cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc
    18001 taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca
    18061 aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag
    18121 caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca
    18181 tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg
    18241 tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga
    18301 cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta
    18361 tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt
    18421 acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac
    18481 ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc
    18541 gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt
    18601 atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt
    18661 agataactct agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag
    18721 tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg
    18781 tattgaatag atcttcatct acccctgatt caagatcgat actttggatc atttcttcac
    18841 agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg
    18901 ctggtggacg caaagtactg actttcttaa gcattgcccg atattttttt gttccttctt
    18961 caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat
    19021 caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt
    19081 ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt
    19141 tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag
    19201 tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa
    19261 ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg
    19321 tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga
    19381 tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa
    19441 aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg
    19501 gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat
    19561 tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct
    19621 ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc
    19681 gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca
    19741 agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg
    19801 tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg
    19861 agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc
    19921 gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa
    19981 acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat
    20041 tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg
    20101 taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat
    20161 tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga
    20221 tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga
    20281 ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca
    20341 gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct
    20401 ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg
    20461 ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta
    20521 ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt
    20581 ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg
    20641 cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact
    20701 gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa
    20761 tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat
    20821 acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc
    20881 agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa
    20941 gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat
    21001 aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc
    21061 tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc
    21121 tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac
    21181 ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt
    21241 aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc
    21301 tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta
    21361 cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat
    21421 atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt
    21481 gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct
    21541 tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc
    21601 aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta
    21661 gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt
    21721 cctacattga taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat
    21781 ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact
    21841 gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag
    21901 acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca
    21961 agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct
    22021 gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc
    22081 ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca
    22141 cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc
    22201 accccaacac cagctcttga aattgctagc aaatctggtg taagccattc atctttcact
    22261 ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc
    22321 ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata
    22381 aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat
    22441 ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag
    22501 gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata
    22561 tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt
    22621 gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc
    22681 tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga
    22741 tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa
    22801 tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta
    22861 ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta
    22921 ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc
    22981 gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca
    23041 gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct
    23101 agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc
    23161 aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc
    23221 atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta
    23281 gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga
    23341 gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta
    23401 tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt
    23461 acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct
    23521 tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact
    23581 tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt
    23641 tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca
    23701 ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata
    23761 tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt
    23821 tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg
    23881 atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca
    23941 gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt
    24001 tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa
    24061 tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac
    24121 tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta
    24181 tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc
    24241 atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc
    24301 caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg
    24361 cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt
    24421 tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc
    24481 gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga
    24541 gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta
    24601 tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg
    24661 tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt
    24721 tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag
    24781 tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt
    24841 ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat
    24901 aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg
    24961 tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata
    25021 ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac
    25081 tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc
    25141 cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat ccattccgat
    25201 tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg
    25261 gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc
    25321 ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt
    25381 gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc
    25441 ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc
    25501 tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc
    25561 tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtccgttt
    25621 ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg
    25681 gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt
    25741 gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac
    25801 gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt
    25861 tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt
    25921 aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc
    25981 ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta
    26041 ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg
    26101 aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact
    26161 gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc
    26221 catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat
    26281 accattttct agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga
    26341 atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc
    26401 atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc
    26461 ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact
    26521 tttgtacgta catgtcaagc ggttatttac aagaatacaa taaaaaacac ttgttttgta
    26581 cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt
    26641 ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt
    26701 ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt
    26761 acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac
    26821 gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa
    26881 gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct
    26941 ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg
    27001 tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc
    27061 tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct
    27121 ttcctatgtt gcagattatt tgctaaaagt gttgcctgaa acgatggctt ggcgcgtgat
    27181 gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga
    27241 gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta
    27301 tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca
    27361 agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt
    27421 cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta
    27481 ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg
    27541 gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga
    27601 caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttcattcat
    27661 cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt
    27721 gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat
    27781 cataggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt
    27841 taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc
    27901 tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca
    27961 agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa
    28021 aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg
    28081 tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa
    28141 aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt
    28201 ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa
    28261 cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc
    28321 gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc
    28381 ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt
    28441 tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt
    28501 tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat
    28561 taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa
    28621 ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc
    28681 ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta
    28741 ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat
    28801 caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt
    28861 taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac
    28921 ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc
    28981 taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt
    29041 actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat
    29101 cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt
    29161 caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt
    29221 acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac
    29281 gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc
    29341 acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc
    29401 ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa
    29461 tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt
    29521 gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc
    29581 ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg
    29641 aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata
    29701 ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc
    29761 acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt
    29821 tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt
    29881 catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt
    29941 cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat
    30001 atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt
    30061 ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta
    30121 cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt
    30181 aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg
    30241 gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg
    30301 actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat
    30361 aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat
    30421 aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta cattccaaga
    30481 gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc
    30541 aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa
    30601 tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg
    30661 ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact
    30721 gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg
    30781 tttcaatact tgacctttag catatttcga taagtgttgc atcacataat aagttggatt
    30841 cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga
    30901 ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa
    30961 taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc
    31021 cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt
    31081 tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg
    31141 acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg
    31201 aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc
    31261 tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg
    31321 tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt
    31381 tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg
    31441 accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt
    31501 ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt
    31561 ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac
    31621 atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca
    31681 tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata
    31741 accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata
    31801 agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat
    31861 ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat
    31921 ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc
    31981 ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa
    32041 gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca
    32101 acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg
    32161 taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga
    32221 ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa
    32281 ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa
    32341 atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca
    32401 ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
    32461 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
    32521 aaatttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
    32581 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
    32641 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
    32701 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
    32761 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
    32821 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
    32881 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
    32941 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
    33001 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
    33061 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
    33121 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
    33181 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
    33241 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
    33301 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
    33361 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
    33421 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
    33481 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
    33541 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
    33601 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
    33661 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
    33721 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
    33781 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
    33841 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
    33901 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
    33961 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
    34021 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
    34081 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
    34141 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
    34201 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
    34261 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
    34321 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
    34381 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
    34441 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
    34501 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
    34561 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
    34621 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
    34681 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
    34741 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
    34801 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
    34861 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
    34921 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
    34981 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
    35041 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
    35101 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
    35161 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
    35221 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
    35281 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
    35341 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
    35401 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
    35461 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
    35521 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
    35581 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
    35641 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
    35701 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
    35761 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
    35821 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
    35881 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
    35941 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
    36001 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
    36061 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
    36121 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
    36181 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc aattgagcaa
    36241 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
    36301 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
    36361 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
    36421 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
    36481 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
    36541 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
    36601 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
    36661 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
    36721 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
    36781 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
    36841 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
    36901 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
    36961 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
    37021 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
    37081 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
    37141 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
    37201 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
    37261 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgt
//
LOCUS       DACDID010000011        35141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDID010000011 DACDID010000000
VERSION     DACDID010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.011"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..369
                     /locus_tag="IV236_000436"
     CDS             37..369
                     /locus_tag="IV236_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8770659.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            960..1289
                     /locus_tag="IV236_000437"
     CDS             960..1289
                     /locus_tag="IV236_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770660.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1276..1617
                     /locus_tag="IV236_000438"
     CDS             1276..1617
                     /locus_tag="IV236_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8770661.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1731..1970
                     /locus_tag="IV236_000439"
     CDS             1731..1970
                     /locus_tag="IV236_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770662.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2037..2276
                     /locus_tag="IV236_000440"
     CDS             2037..2276
                     /locus_tag="IV236_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770663.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            2362..3033
                     /locus_tag="IV236_000441"
     CDS             2362..3033
                     /locus_tag="IV236_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8770664.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3086..5062
                     /locus_tag="IV236_000442"
     CDS             3086..5062
                     /locus_tag="IV236_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8770665.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            5075..5731
                     /locus_tag="IV236_000443"
     CDS             5075..5731
                     /locus_tag="IV236_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8770666.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            5827..6654
                     /locus_tag="IV236_000444"
     CDS             5827..6654
                     /locus_tag="IV236_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770667.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            6664..6912
                     /locus_tag="IV236_000445"
     CDS             6664..6912
                     /locus_tag="IV236_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770668.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            6943..9054
                     /locus_tag="IV236_000446"
     CDS             6943..9054
                     /locus_tag="IV236_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8770669.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(9092..11149)
                     /locus_tag="IV236_000447"
     CDS             complement(9092..11149)
                     /locus_tag="IV236_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8770670.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(11263..11475)
                     /locus_tag="IV236_000448"
     CDS             complement(11263..11475)
                     /locus_tag="IV236_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770671.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            11662..12129
                     /locus_tag="IV236_000449"
     CDS             11662..12129
                     /locus_tag="IV236_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770672.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            12126..12368
                     /locus_tag="IV236_000450"
     CDS             12126..12368
                     /locus_tag="IV236_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770673.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12386..12688
                     /locus_tag="IV236_000451"
     CDS             12386..12688
                     /locus_tag="IV236_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770674.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            12759..14813
                     /locus_tag="IV236_000452"
     CDS             12759..14813
                     /locus_tag="IV236_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770675.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            14813..16645
                     /locus_tag="IV236_000453"
     CDS             14813..16645
                     /locus_tag="IV236_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8770676.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            17340..19256
                     /gene="ltrA"
                     /locus_tag="IV236_000454"
     CDS             17340..19256
                     /gene="ltrA"
                     /locus_tag="IV236_000454"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8770677.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            19373..20173
                     /locus_tag="IV236_000455"
                     /pseudo
     CDS             19373..20173
                     /locus_tag="IV236_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            20170..22701
                     /locus_tag="IV236_000456"
     CDS             20170..22701
                     /locus_tag="IV236_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770678.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            22751..23083
                     /locus_tag="IV236_000457"
     CDS             22751..23083
                     /locus_tag="IV236_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770679.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23084..23677
                     /locus_tag="IV236_000458"
     CDS             23084..23677
                     /locus_tag="IV236_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770680.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23840..25534
                     /locus_tag="IV236_000459"
     CDS             23840..25534
                     /locus_tag="IV236_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8770681.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            26222..28099
                     /gene="ltrA"
                     /locus_tag="IV236_000460"
     CDS             26222..28099
                     /gene="ltrA"
                     /locus_tag="IV236_000460"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8770682.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            28230..28607
                     /locus_tag="IV236_000461"
                     /pseudo
     CDS             28230..28607
                     /locus_tag="IV236_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            28607..29812
                     /locus_tag="IV236_000462"
     CDS             28607..29812
                     /locus_tag="IV236_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8770683.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            29828..30472
                     /locus_tag="IV236_000463"
     CDS             29828..30472
                     /locus_tag="IV236_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770684.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            30483..31289
                     /locus_tag="IV236_000464"
     CDS             30483..31289
                     /locus_tag="IV236_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770685.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            31311..31541
                     /locus_tag="IV236_000465"
     CDS             31311..31541
                     /locus_tag="IV236_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770686.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            32087..32362
                     /locus_tag="IV236_000466"
     CDS             32087..32362
                     /locus_tag="IV236_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770687.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            32404..32826
                     /locus_tag="IV236_000467"
     CDS             32404..32826
                     /locus_tag="IV236_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770688.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            32845..34437
                     /locus_tag="IV236_000468"
     CDS             32845..34437
                     /locus_tag="IV236_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770689.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            34449..34784
                     /locus_tag="IV236_000469"
     CDS             34449..34784
                     /locus_tag="IV236_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770690.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            34931..35125
                     /locus_tag="IV236_000470"
     CDS             34931..35125
                     /locus_tag="IV236_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770691.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
BASE COUNT        12917 a         5443 c         7173 g         9608 t
ORIGIN      
        1 gttaaaaata tttaaagtaa gtgggtggat tattttatgt catatacagg aattttgagt
       61 ctcgaagata tttgtcacta cggtaaacgg tgtaccgcta cagaaaagat cacgaagaaa
      121 ctatcaactg gtcaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
      181 aaagtctcag aagaaatgat ttattacatt gggaaacaaa aacaaataat tttaaaagat
      241 cctatccctt taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
      301 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgttta
      361 atcagctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
      421 attgtcgaga cttctaaagg aacttagaat cactagaaaa ataaggagta gacttgatct
      481 atcaacaatt cctttcgggg ggtgacgggg taggttttta cgattggtaa cgtaaaaaat
      541 tggtcaatcg agtaaatgcc cttgtttgga tagtggtgaa atgaatttgt gtaaataata
      601 tgcacagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gcctagatta
      661 atcatgagca gctactaagg gatctaggct ttgacgcaaa ctcatggaat aaggagtagg
      721 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
      781 acttgcgaag acgttgatct ttggatcaat cgagcattta ccgttttcta ctagggtttc
      841 tcgtttcgga cgccataaac gagtcgattt attttatata tttttgagtg aacgaaaaac
      901 gataaaaata ttcgttatga agtaaaacta atttagtaga tagcaaaggt ggtagaggaa
      961 tgattcaaaa atttgcgaaa atgagtaaaa ctgcatggat tgaaaagctt gaaaatgact
     1021 tgagtgtcat gcgattagct ccaaagaaat tcaaacgaga gattgcagaa aaagaagcaa
     1081 ttttgtcggt attgaatgcg gtagacgata aatgtccagt aaaaattagt ttttcagata
     1141 tagcaacaat tatcgaaggt atttattatg gtgaagttga tgaagagata tcttctacaa
     1201 aagatttatt agattttttg gataaaaaca aagatacctt tatagtacta actgattatg
     1261 aggaggttac aacaaatgaa aaattatgat ccgaatagcc gatggggaac acatacgatt
     1321 aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag aggcggaaat
     1381 tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca aaaattgact
     1441 gataatccaa ttggattcgg cttgctacct gaagatgatc aaggtaatga atggtttaaa
     1501 atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg
     1561 agtgattata ttgtcagcgt tgaaatcatt gaatttgtag cggataaaga agaataaaaa
     1621 ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg
     1681 tagtattacc ttaggcattg agtaaggaat ttatttagga ggaaaattag atggcgcgac
     1741 cagttttaga ggcgatggat atggatactt tgacaaaagg aaggtatgta cagctgttag
     1801 ctcgtggtta ttcacctaaa gaagtattca aatccctgtc taaaggaaca gatatggaaa
     1861 aagaaagatt acgaaaagaa ttcgattatt ggagaaccca taatggaatc aaggatttga
     1921 aaccagcacg gccagtaatt aaacgaaaga aaaagataac ccaagactga agtgtagagc
     1981 gtatgtgatc ataggctttt ttatttttgc aaaaaacaag gaaggtagga aaactcatga
     2041 attatgaaga aatcgaaaac agaaaaaaag tgtcgaaaga aatggaagaa aagttgctaa
     2101 aaacgatgaa acaaaaacac cttaaaagat tatctgtcat gcaatatatc aatgatatgc
     2161 agataacagg aaaagagaaa gcttgtttgt taggttcaat gaaaaacttt gaacagttga
     2221 gacgaactta tgtgaaaaaa agttctaatt gtcaactatt gttagaagtc tcatgacatt
     2281 cattaataga attcattttt aaaacttttt ctatggaaat gagaaaaaga ttttttgttt
     2341 tgtctgaaag ggggtagctg gatgagaaaa tggttatttg gttttttagg agtcgcttta
     2401 atcgtctttt gcagtgtgtt tggctatgtt agctaccaaa aacatgaagg aaaggtgttt
     2461 aagcaaaata tagaaaaaaa gatgcccgta gatcaaatca atgaacatgc aaagagttat
     2521 aaggaggatg caacgaacgt caataatgat atgtctttgg ggcaaatgtt gtccatacaa
     2581 aaagaagcaa tcgaaatggg tgtcaataaa caagcctttg cacaaattca gatacctgca
     2641 cttggtttag cattgccaat ttttaaaggg gcaaatcaat acactttgag tcttggagcg
     2701 gctacttatt tttatgaaga tgccgaaatg ggcaaaggaa actatgtcct agcaggtcat
     2761 aacatggaaa tgccaggtgt tttattttct gatattcaaa agttgtcatt aggggaggtg
     2821 atggacttag ttagtaacga tggcgtctat cgttacaagg taacgcgtaa atttatcgta
     2881 cctgagtact ttaaattaat cgatggcgtt cctgaagaga actctttctt gtctttacca
     2941 aagaaaggag agaaaccctt attaacgttg tttacttgtg tttatacgtc acaaggaaaa
     3001 gaaagatacg tggtacaggg agagctacaa taacaaggtt ttaatcaacg cgagtcgttg
     3061 aaatatgaaa tgaggagagt aaaaaatggc aataaatcgg tatgtaaaaa aagctggaat
     3121 gcttgttgga atgttaggaa tcattgcaag ctgctttcag gggtatcaag tacacgcaga
     3181 agatgtcacg caaaaaacac caccagagaa ggtcaatatc acggttcata aattaatgta
     3241 cgatcaagga acgcaattga atgttgatat tgacgggatc aaaaacgatg gttatacgca
     3301 cgatcaatat cctacaggtg taacgaaata caacaaggca gattatggtg atgttgagtt
     3361 tacgttagga aatattacgg atcaagtatt accgagagaa gattctgatc taaccaacgc
     3421 gaaagtggac gagattgtac aagatgtaga agacaaggga tctaacagtg agtacgtcaa
     3481 gaatgcaacc aacaaaatta cttcagctgt agatgaaaat ggtgagatca cttttactga
     3541 tcaaccagcc tatgtcaatt ccaaagggaa tgtatatgtg gtttatgaaa gtaaatccgc
     3601 tgctggatta gtcacacaaa aagcaaaacc aatggttgtg attgcgccaa tgacagataa
     3661 aacaggatca ggatttttaa aaaatattca tttgtatcct aagaacattg tttctaagtt
     3721 gagttttgaa ttaacgaaat ttggtgacga tggcgcagcc caaagcaaac aaaccctttt
     3781 aaaaggggca aaatttgagt tatacaaggg cgaacctgga aaaggaacaa aattgggtga
     3841 tcttgtatcg gatgatcaag gaaagctgac tgcgactgat ttgactttag gaaaatacta
     3901 ttttgtggaa gttccttcgg aagttgtagt gggatcagat caagaaccaa cagcagatca
     3961 atatttatta ggcgcagacg ctcgcaacga tgcgcataat aaattaacct ttgaaattac
     4021 caatgacggc gtgacaagtg acttaaaagc ttcctacgtg aactataaag caccagtgat
     4081 cgataaaaca gtgacaaatg gaacaggaca agaacattct ttccaaattg gtgacgcagt
     4141 gaattatcaa ggaacgattc atatccctac agatattgca ggtggaacag atgggattac
     4201 agtaaatggt gtgaagagtg aaacttctcc ttacagcgta tttaagtggg gagatacagc
     4261 aggacaagga ctttcttatg tcgctgctaa agcaaatatc aaagtaacaa ataaagatgg
     4321 atctgtggta ttaaaagaaa atacagatta taaaatccaa aattcagaaa atggctttgt
     4381 gatcgatttt atcgtgaata atggccaagt aagtgatact gtagcaagtt tacacggaca
     4441 agatcttcaa atgacttata acatgtatgt aaatgacagc gcagctgttg ccaatccatt
     4501 aacaaatagc gtggatttcg tttataacaa caatccattt aatcaggaag aacaccacga
     4561 aaaaacgaaa gctgacgttg tgacttatgg tgcaaaattc ttaaaagtcg atagcggact
     4621 atttggaaca ggaatcaaag caacaccgct tgaaggcgca gaatttgctg ctaaaaacgc
     4681 agaaggaaaa tattatggtg gattagttga tacggataaa gacggtgtca aagaagcagt
     4741 atgggtagac gatgtagcaa atgcagcgat cctaaaatca gataaagaag gtcactttga
     4801 aatcactggt ttaactgaag gtgaatattc actggaagaa acaaaagcac caactggtta
     4861 ccaaaaatta acagaagata ttgcatttaa agtaaacaaa aactcattta aagaggaaaa
     4921 tcgaatcact gttaaaaata atcaaaaagc agctattcca ttaacaggta gtaatggttt
     4981 ccaaacgtat gtattgatct cttgcttgtt gctaggagca ggggcacttt ctgcagttgt
     5041 ttactttaag aagaaagcct agaaggatca gctaatgatt attcgtaagc ccccaaaaag
     5101 aagagtcatg cacaaactga tgatattttt ttggttgctg tcggcagtag cagtctattt
     5161 aattccagct gtggaaacaa atgtagcgaa atttcaacaa cagcaaagaa ttttagccat
     5221 tgaaaatctt gaaaaaacag attcttcttt ggatgttgcg aaaatagagt taggcgatcc
     5281 tgttggtgtt cttacaattc caagtattcc tttaaaacta ccaatttatg acggaacttc
     5341 cgacaaaatt ttagagaatg gtgtagggat tacggaagga actggcgata ttacaggagg
     5401 aaacggtaaa aatcctttaa tcgcggggca cagtggactt tataaagata atcttttcga
     5461 tgatttgcct tcagtaaaaa aagggcaaaa attctttatt aaggtaaatg aagaacaaca
     5521 tgcctatcaa atttatcgaa ttgaagaagt taaagcagaa gaattacaaa aaaattattt
     5581 agattatctc atgcctgaaa aagaccaaga taggatcacg ttaatgacat gcacgccaaa
     5641 aggtgttaac tcacaccgct ttttagtgta tggtgaacga gtgtcattca gtaaagaaga
     5701 tttaaaacta cagaaaaaag caaaaaaata ggaaatcaat ttcattggat tggcgattag
     5761 cactgctatt cgcttttttt gcgttcctat tatttttatt tataaaaaaa taaggaggag
     5821 atcggaatga ataagagaaa atggctgttt gtttgtgtag cagctctctt tgcaactgca
     5881 gcttactcac agatatcttt tgctgatagc agtactactc caactaccag tcagacaaca
     5941 gatagttcaa caactgcaac aagtgacacg gacagttcga agaacactgc agaagaaacg
     6001 gcagattcaa gtacgagttc tactgaatca gcggaatcaa cgtcagaaag ttcttcagag
     6061 acttcagaaa aactgcaaga agtagaagat acgcaagtaa tcaagcaaaa cattcatatt
     6121 caaaaaatta tttcttcgaa tcgaatcaac aatcaaggaa ggcaagtgac tcagcaagaa
     6181 ggtgtcaatg gtgcaaagtt tattgtctac gatatcacga atatcttaga agaaatgaca
     6241 caagatgata atgagaaaaa tcaatcggta gaaataattg agtcaaaatt gaaagatcga
     6301 gcaaaaaaac tttcatacga tcagttgaaa aaagtgactg aaggagaaac ccaaacaatt
     6361 gatgatcagg aaggcgtgat tgatttttca attgaagtac ctgcaaacaa aaaacaagca
     6421 tactatatcg tcaatgaatc aagccctgaa aacatttcaa atagcgaaga tattgtttta
     6481 ttgacacctg tttcagatga aaagggtgaa tttttgacgg atgtttggtt atatcctaaa
     6541 tcaaagaaat cagagccgaa agaagaaatt aaaaaagttg tatcaactgg tgtaaagaaa
     6601 aacttttttg ataattgttg ggattttttc actcatttat tttttaacga ttaggatgaa
     6661 atgatgaaaa taataaaaat gttagttttt aatctatctt ttttagccgc aacaatattg
     6721 tgttgcggtt tagttttttc tcaattatca atttacccaa gatatcaacg attgttcgtt
     6781 cattttctaa atatccagca tgcttccatg ccactagaag tgattaacaa gctcttaatc
     6841 aatccaattt ttatggctag tttgtttctt cttgcaggat tggtaacgtg ggtattagga
     6901 aaaaaagaat gagaagctta caatcgttta acaaaataat agatggaggg cattatggaa
     6961 aataaaattc attgcactac gggaagagta tttactttag tgttgttttt gttccttctt
     7021 ctttttgcaa ggggaactgc agtccaggcc ttagaaaaag ttgatccgag tcaaatcaaa
     7081 gaaaataata atctggtact ttatcatgat agtgattcat tgagaaatat cgaagtaact
     7141 gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa agtacttgct
     7201 tggccaaaag tcaaagattt gaaaagcacg tcaaaaattg ttgcccatta tcaaaaggtg
     7261 ggcacttatc taggaaaaga aattgattgc gacgtaactt tttctaattt tgtagcgcac
     7321 gctggggtta accaagacgg attagctaaa acaattaatg ggaaaaagta ttataattat
     7381 ttgctctttg ataaaaacat ttatcgaggc tttttcatgc agtcaatcaa cgttatgaat
     7441 gttaaactag attttaagta tgcaggatca aaaacaaaag tagctttgag tgaaccacag
     7501 ccagaaatta attcaaaaat gagatcgatg agagcagctg gtattgagtt gaatgataca
     7561 tttctatcta tttacagttt aaatggacag tatcagcaac gtgtgtcttg gtataatgac
     7621 aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg gtacgaaaaa
     7681 atggatcgct taccttattt tgtcacaaca gatacagcat gtgcagaata caaagctcct
     7741 gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga aaaggcttat
     7801 agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag aagtggtgta
     7861 acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac gaatagtatg
     7921 atgtttagct ttgatactgc attgctttgg acaccacaac catcgaagcc tacaaaagaa
     7981 gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa agtgattcca
     8041 caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac agtcaacaca
     8101 atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa ttttgatatg
     8161 gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa tgggacttgg
     8221 aaaaaagctg atacaggagg aaaattaact gtatcaggta atacagtcac ttatactgct
     8281 tctgacagct ttttagctaa tgctgcaaat tacggtaggg actacatttt gcagctccat
     8341 atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt aacgtttaac
     8401 aatgatccga acaaaggaac acaaaaaaca aataaagtag aaaactattt ctctcttaaa
     8461 ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga tggtaaaaaa
     8521 ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt cacacaaaaa
     8581 tggcatacaa aaggtgtaga cattgcgctc gatcattaca agcaatttag tattcaagat
     8641 ccagtggaag ctcgtttgac gtataaagaa ggttctgcgc gagtgataga tatgagtaca
     8701 ggaaaagata ttacttcaga aggtatacta acctatgata gccattcaag aacgctgaaa
     8761 tgggaagctt ccgctgattt cttaaataac aatcttttag atggacgaga aattcaactg
     8821 atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca agcaaaagtt
     8881 tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa acctaactta
     8941 cctcaggtca ttgtgccaaa aactggatcg aatcatttag tgctaactgt tattggaagt
     9001 atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa ataaatataa
     9061 aaacaaggct gattttcagc cttgttttct tttatttgaa gattggattc gcactaataa
     9121 atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga cctaatccga
     9181 gaatactttg gttattataa agtaaatttg cattatgggc agggctattt ttccatccta
     9241 ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc ccagtttgac
     9301 ttatgttttc tcccttagca ccatagcagc ctaagctttc caataaatct tggaaagtac
     9361 caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga atatctgcag
     9421 cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg cgataatcat
     9481 ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat gaaatctttt
     9541 tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt tggccgtttt
     9601 tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga tcagtcatat
     9661 ttttccaagg agtagagaag ctatcccctt cgtatactgt ttttgattca ctagagagaa
     9721 cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga gtgtagttga
     9781 aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt ggttctaccc
     9841 gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactt tcaccgtgag
     9901 tgacgttgat tatctgttct ttcaaaatat caactcgccc ctcatttttg tagttgttat
     9961 tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt gtattacctg
    10021 tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta tctttagcac
    10081 cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa gtcacttttg
    10141 cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaatg gtaaccatat
    10201 attcttttgg cgtttcaggt ttgtgttgca accctttaaa tgcagcatca agactatttt
    10261 tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt ttagcattgg
    10321 ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta gcagttgatt
    10381 ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact ggttttttct
    10441 ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc ttttgagtcg
    10501 cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat gcgttccagg
    10561 tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga gcttctagag
    10621 ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata ccttcgtatt
    10681 tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg tagtgatgtg
    10741 agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt ttttcggcag
    10801 aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat ttcacttcat
    10861 cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga tagacaagat
    10921 ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat tttatcttta
    10981 ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta tttggattgt
    11041 aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga ctaagtgagg
    11101 cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt ttttcaactc
    11161 ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggttaaccga tatagtcaat
    11221 aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt tagttgaagt
    11281 attaatatca acgatttctt cagccaattt atttaatttc atagcattta tgaatctcat
    11341 gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg gtacgccaga
    11401 ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt cgattttctt
    11461 tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc tcttttttgt
    11521 aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt atgagtattc
    11581 gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat actaaaaata
    11641 aacaacgaaa gaaggaataa aatgaagaaa aagcaactat ggtttgtctc tatgtttgca
    11701 atatttttta ttattttaag tggatgttca agtaataaga aatcggaagc taaagaagat
    11761 aatagtttat tgcaaccagg aactgtttgg aaagaagaag taggtggtct tattcataat
    11821 ttaaaaatta tagatgatac tacctgggaa tattctgaaa gtgtatggca tccagatcct
    11881 gtacaaataa cagttaagag gcaaaaagac tatgaaggtt tagaaagata caaaatagta
    11941 gattctgcag gcattaggga atttataaac aaacctgatt cattatttat tgttgttccc
    12001 tatgagaagg atggcgtaaa aaaaataatt tttcttggat ctagcaagga tgagaagcga
    12061 acgaaagaga aattaattca tgatggatca caatcaaata aatacaaatt acaaaagact
    12121 agcgagtgat tgctagtctt tttatttgtc ctgaaggagg aatcacagtg gcaaaagcta
    12181 aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg ccgcggtgta tctgaaacac
    12241 agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg
    12301 acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta
    12361 aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca
    12421 accgaaagta gtagctgcgg ttgatatttt tagagcatta caaactgccg caactagttt
    12481 gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg gcttccattg ctcttggagg
    12541 tattttcttc ttctttgggc gaagaggagc tgaaaacggg aagacacacg taggttatgt
    12601 attgttgggg gtaggagtta ttttagctgc cgcttctatt gtagtttcaa tgttctcaat
    12661 gtttggacaa accccacaag gactataaaa aaacaagtag aaggagtaaa aagtacgtgg
    12721 ctacttttta ctcctttttt tatggaagga gaatattcat gttttttacg atttcaggca
    12781 atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga aagcgtactt
    12841 cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
    12901 taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc acgttacatg
    12961 ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggctca ctggattatc
    13021 aaatttcaga aggtttttat cctacattga ttgatttaat ccgcctagat atatttaaag
    13081 atacggatga aaccatgagt gtggaagagt cttatcgaaa cgaaaaaatt ttacaagttt
    13141 tagaagaaga gttagaaagc gaccaaagaa aagtgaatca agtgagtgaa gtcgaagcaa
    13201 acgaagaagt accatgggaa cgcccaatct ttccaaagga tgaatcaaaa gaaaaagaga
    13261 aaggaattat tgacgaacaa gctgacattt tgactcataa atcaatattt gaggaagaga
    13321 acgaggtaat gggtcaattg gaggtagatc cctttgaaga aacaatggag gatgaagaga
    13381 cggccgaagt agaagttgat ccagttgaag ctaatgaaga cccaacatgg actgaagggg
    13441 attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaat gaaacagaga
    13501 tacaaaaggc aacggtattt gattatattt tggatcaata tccgccagag catgaatgga
    13561 tgaaagaaaa aaataaatta tttttaaatg aactttattc gaaatatcaa ttacctcaaa
    13621 cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa ggcacacttt
    13681 tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa gaagatttat
    13741 tagctacttt tgaagaacgt caacaagaaa cagatcaaga aattaatgag tatgcgacgg
    13801 aacagtcgtc gaattgggag aaagcaaaag aggaactaaa taaagaagaa cagcgagtga
    13861 ttgaagaaga agtacaaaaa attaaagagc ggtacgaaaa gaaacgtgac aatgcgtatt
    13921 ccttgtgtgt agaaaagatc aacaatttta ctcagacgaa tcaagaagca ttatcgaaag
    13981 aaaaagaaaa cttattaaaa gaacgtataa aagaacgaaa agaagaattt taccgagggt
    14041 tgcgtactga taagctaagg attagcaaag aattaaatga acaactgaat aaactctatc
    14101 aaactacgac tgaagttctg atggaaaaac acgagcagat ccaacaagaa ttacaagcac
    14161 aaattccaaa atggaaaaaa gagtatgaaa ttcaccaaca agaattgaaa gaacaagaag
    14221 aaaaacaacg gaaaaaagaa aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg
    14281 atcttgcgga acaaaaaatg gcgatagaaa aacaacggct agaacaagaa aaggaaaaag
    14341 aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgacaa gcacagttaa
    14401 attatctgac acatcctcaa ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa
    14461 atttaactcc ttcaccccag cgttccttta acggatggat ggtgggatgt attgcaagtt
    14521 taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat caatcttcac
    14581 aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa caagcaagaa
    14641 aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca acaacagagt
    14701 caagcatgga aaatagcaaa gagagtgatt ctagtaaggc taaagaagga tcaagcgatt
    14761 caactgtaca atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt
    14821 tagagaaaca acgaagcgaa aagacaaacg attcaattta gaaaagaata ggtatctttc
    14881 tcatttctat cgctatactc cctcaggaga agagctacca gaaaaacatg acaaacgcat
    14941 ttcgaaaaag attttttata gtggacttgt atacctccct ttagagtatt tagtaacaag
    15001 agtaccagaa gttttcaacc gattggaaat tgttaaggga agcttacaag gaataaatct
    15061 gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt tttggaatcc
    15121 atttactcct acaactagtt tgggtacacg tttagcaatt tcgacagttt gtgtggttcc
    15181 agctgtactt atgggattga aaatggacat gtggtttcgt cctctagcag atggacaaaa
    15241 aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc aaatacctga
    15301 aacaaaaact acttttccag gttatggagg tattccaatc acacattaca aaaaatattg
    15361 gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat cagggaaagg
    15421 tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac aaagttcaat
    15481 ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac tagaagctag
    15541 agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga gtgataatcc
    15601 attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa cacagttaac
    15661 caatactttc acaaatacga tttattatga tgccaacgct tcagataaca aatactttaa
    15721 cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac aagctgatca
    15781 aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt ctgagattgg
    15841 cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg ccttagatga
    15901 atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat
    15961 tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat tgaatccatt
    16021 tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata aatcggttgg
    16081 gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt
    16141 agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag gctataaagg
    16201 atttagtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt gtttaaaata
    16261 cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag tattgaaaga
    16321 cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc cagaattaga
    16381 aaaagaagcc gttctaacat tagatgagga aaaaagtaac ttattagtag agtctattcg
    16441 gatgcactat accgataaac caatggctat ttttatgctt acacctgatt atgatccatc
    16501 taaccatatc attgttagta tttttcttag tcagttatat aaagaattat ctacgcaatg
    16561 tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag atgaatttgg
    16621 gaatagtgtg acctgtttag cctaaggata aaagaggcta gttccaacga acattggaat
    16681 aactttacta tcgagaaatc aaatgacggg gtaacgccct gaagggtttc cactgatact
    16741 gcgacatgcg ttagtaggat gtgcatccaa acgcgtatga agctcgctga agtcggcaga
    16801 agggtaccgt aaggaaagac acgatacgta tctaccacgc aagtgctcta gaggtagggt
    16861 atgtacctga tatgtcgggt gtcctaaaaa gttcaataag gtgagaatga tttgattatc
    16921 tgacaaactt gcgaatggaa tgtctgaaac gaataactca tagaaatgtg agggggaagg
    16981 gggatacgtc ctctttccat tgactcgagg ttgagtaaaa atgtgtttct atgaaaaacc
    17041 ttgcatggtt aaaggctatg gagtagtcaa caggcaaaag caagcaccta agttgaaatc
    17101 tatggataga tagtaaggaa caggggaagc atctagcacg gagggagcat accgaagatg
    17161 tgtttctaag gaaaaaccga aatcggttat aacttaggtg aatagatgtg aaagtagagc
    17221 cacgaccagt gaaaatttcg taatgaaatt ggaggaacag gctcaagtcg ttatttataa
    17281 tataagaaac ctatttaaaa tcatggattc tcgtaagact aaaaaggtca cgaaccagaa
    17341 tggagcgtga taaaaccatg actaacacaa aaggaattag cttaaagaac aaacaactca
    17401 gacacgccga atactacgac atgactgaga ctttcgatga attgcacgca atgagtgttg
    17461 caaaatcaaa ttttaaaaat ctcatgaaat tcattattga tgacaataat atctttctag
    17521 cgtatagaaa tatcaaaaag aataaaggaa gtgcaacggc cggatacgat ggtgtcacta
    17581 taaaagacat tgaaaaattg gcatgtggtg actttttaac tattattaaa aggcgattta
    17641 aggattacag accaagaaaa gttaaaagag tagagatccc gaaaccaaac ggaaaacttc
    17701 gacctttagg aattccttca atatgggaca ggatagtcca gcaatgtatt ctacaaattt
    17761 tagaaccaat atgcgaagca aaatttaata agcatagcta tggattcaga ccaaattgtt
    17821 ctacagaaaa cgccatagca gattgtcttg ctagaatcaa tcaaagtaag atgaattatg
    17881 ttgtagatat tgatataaaa ggattctttg acgaagtaaa ccataccaaa cttatgcgtc
    17941 aaatatggac aatggggatt cgagataaac aactccttgt aattattcgt aagatgttaa
    18001 aggctccgat tgttatgcga aatggcaatg ttgtttttcc aaccaagggt actccacaag
    18061 gagggatttt aagtccactg cttgcgaata tatgcctgaa tgaatttgat tggtggatag
    18121 ctaatcaatg ggaggaacga actgtcaaag agctgaaaca ccactatcga aaaaacggag
    18181 catgggataa aggaaatatc tattcgaaat taagaagcca cacaagttta aaagagatgt
    18241 atctggtacg ctatgcagat gattttaaaa ttttctgccg aacaggagaa gaagcccaaa
    18301 gggcttttta tgccacacaa aaatggctca gagaaagatt aaagctccca atttcaatgg
    18361 agaaatctaa agttacgaac ctaaataaag ctgaaagtga attcttgggt ttttcaataa
    18421 gactccacaa aaagggaaaa aagaaagtgg ctttcacgta cgtttgtcgc aaagcagaag
    18481 aaaacattaa aaataaacta aaacaacaga ttaaaaaaat tcaatacgct ggagcaaaaa
    18541 aaaataggca ccgagcaata ggtgactaca acatgatggt catcggtatc cacaattact
    18601 atcggatagc ttcacattgt agtgtcaatt tcactgagat ttatagaagt gttaggacag
    18661 taatagaagc aagactgaaa gctagaggaa tgacgaaaaa tggaatctat ggacaagatc
    18721 atgaaggata ccgtccgtat ctaaagtcta agcaaatgag atatattgaa ggttttccga
    18781 tacttccaat tggctatatt aaaaaaagaa ctccactcaa caagcggaga aatctaaata
    18841 aatataccaa ggaagggaga aagctcattc atcaagagca acaagctata gaaagctgga
    18901 aaatcaagta tcttgtaaaa ggttttagta acaataagaa atacacggtg gaattttctg
    18961 ataacaaaat atctaagttt atttcccaaa aagggaaatg cgctatcaca ggaaacgaac
    19021 ttttgatgga agaaattgaa tgccatcaca ttaacccata tcatttgtcc aaagacgata
    19081 gttacaagaa ccttgtcatt atctctagag aaatccatag attaatccat gcaactagtg
    19141 aagaaactat tctgaaactg cttcggttac tgaaactaaa tcactatcaa attagaaaac
    19201 taaacacgta tcgaaaaaaa gcagctaatt ttccaatatc aatcaaaatg aattagatag
    19261 gaaacaaatt atattgtaat taacgatgga acgccgtgtg cggtgaaagt cgcacgcacg
    19321 gtgtggaccg ggggaaaaga cggagataat atcaaactct tacctatcgg aattgccacc
    19381 aatggataat atggaaggaa tcatgacggt cacagctggc cgaaacatgt tgtgggatct
    19441 gtttattcaa tcttatcagc aactttatgc gaagtatgga gaacaaaacg cagcgattat
    19501 caaagaaaat tgtcagacac atatttatat tatgtcgaca gatgataata ccatcgaaga
    19561 attttctaaa aaagtgggga ataagacggt tgaacaagag aacagtacca tcaattcatt
    19621 aggtatgaat actcatatca atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag
    19681 aatttctact tttttagaag gtgaaacgat tgtactcgct cctttaaaac gacaagattt
    19741 gaaaggtcat aaaatacggc cgtatcctat ttttaatcat ggggaaacga atcttcctta
    19801 tgcttatcaa tatattgaag atttcaatcc atcgtgggat atcaatgata tagacataca
    19861 ttgtacacat gcggacttgg atcttaaggc tttacaaatt gattatgatt atttcctacc
    19921 agatgaatat gctagaaatc tctatcatgg actgcatgat caattagaag aaaaaaacga
    19981 agaaaatatg ttttataaaa tcgttcaatc tcttgagaat gagacggcta aaaaattgtt
    20041 aattcaattt tacgaagaac aagcagcaga tcgacttcaa gaatatgttg aaaagttgat
    20101 caaaaatgaa gaaatttctg aaatgattgg gaaacggttg ttattggaat ctgaaaaggt
    20161 ggtgaaagaa tgatcattgg aggaattttt tctggcatag ccgactggtg gtcaggctta
    20221 ttcggagaag gggatgcaaa ccccaaaacc gtaacgaaag tcttacaaga ttatggtaat
    20281 tatctgcagt atcaagatgt gttcagttgg atttgtaata cttttttatg ggggttgatc
    20341 aaagggttat ataatctcaa tactttacta gaaaaaacca tttatcaaag ctttgattta
    20401 aaagacttat tgaacgccgc tggggtaaat gaactatttc aaagtttgat tagtaaggta
    20461 ttggctcttt tttgtgtcgt cacattaatt tatttaggct tgaaattttt tatgagtaaa
    20521 cacccaccaa aagtcaaaaa tatcttggtt aatttattta tggccatgat cttgattttt
    20581 ggtggtagtt ctttaatcga tgaagggtta aatatcagta aaagctttta cggagacgtc
    20641 actgcagcaa ataaagcaga taaaacgggt tcacctgctt ttcaaattat tcaaaataat
    20701 gtgatcgatg tcagtacatt actagcaact gacccgaata aagtagaatc cctgccaaca
    20761 gacaaaagaa atgctttaac agcgaaaaat tttaaaacag cgaatatcaa tgggattatt
    20821 actcctaaac aagctaaaga aatggcggat aaagataaga gtggtttatc agatttacaa
    20881 aaggatcggt taagagcgtt acaataccga ctggaactgg atgataatgg aaaagaaatt
    20941 cctgtgcaaa tcagtgatga aggcttagcc aaatatgttt atacttcagg gtatcgtcga
    21001 tacattagta gtccagggat tattttagct ggtgaatctt ctttggcggt ggcctattta
    21061 tttatcctat ttacgatcgt tacttgcatt attgagctgg tatttaaaaa attctattta
    21121 gtgattgccg cgtcaacgga ctttgagaca ggacaacgga tgaaaagtgc tatcgaagat
    21181 atttctcaat ccttcttgtt attggcattt acaattttag aattacgaat ctatacctta
    21241 atgctttcag gtattggtga tttgcatgcg gcaggaaaga tcaatggatt cttgtatgtt
    21301 gtcgctttga ttgtactaac cattgcgcta ttcaaaggct ctcaatcagt tacgaaaatt
    21361 ttcggcgttg atacgagtct gaaaaacggc agtaattctc ttcttagcat gtttgcctta
    21421 ggaaatattg cgaaaaatgt gggtagcgga gctaaaaacc ttgcaagtgc tggtaagagt
    21481 ggtttaagcc atttaaatga tattcgtaaa aatggatttg gccatcgaaa agatgttacc
    21541 cctgataacg gggaagaaca atcagacgat aaagctacaa ctgtacctaa tagtaagaaa
    21601 acagtttccg aaatgttcgg gaaagcgaaa aatggtgttt ctaaagccgc agaaggtctt
    21661 ggttacgtca atgaacgtgg gttaaaagga acagctgaag atcttacaga aaaagccaaa
    21721 gacaccttag aagataaatt agacggcacg agagctaaag aattggctga agctgtgaag
    21781 aatccatccg aagcactaga aaaacaaaaa gaaaaagctt cagaagtcaa agaaggacta
    21841 aaagatgcgt atggtgaagg acaagtcaat gcagcagtga aagctaatac aacaaccgaa
    21901 aaagaaaatc aagaaggtat agtgattcct tcagtcaaag atcaccaaga agcgttgcac
    21961 tctaatgaaa attcgattga agagaaaaat tccatagaag aaagaaattc agaagaagga
    22021 aaaaatggac agttaccaaa aactgatcca gaacaatatc gctataaacc taacagtgac
    22081 cttgaaaatg agaagcgaat ttcagattta tcaaaagtaa atcaagaaga caaagcagaa
    22141 aatgagggac agttaccaaa aactgatcca gaacaatatc ggtatagatc tgttaactcc
    22201 gaaaatgaaa aacgcatttc agatttacca aaacgaaatc aagaagtaca aacggatgag
    22261 gattccatcg ttctgccaaa tgataatgga atcgaagttg gaactgaaaa atcaacgaaa
    22321 attggtttac ctaaggcaca aaatttacca atgaaagata gtcgtttgaa tcaattcaag
    22381 gataatgatc aaagttttgt tcatcaatca cctgcaggta ctgttcaaat gaaaccaagt
    22441 cgtttgcctg aaaatggaaa tctgacaact gtttcttaca atttatctga aagtggtgag
    22501 acagggaatg cgtccgttca aaatgttgtg acaagagaag ctgcaccaaa acctattcaa
    22561 aacgttcata caggttacaa gccagcaaat gtggcattta ctgcaccaac aaaaataaat
    22621 gtgacaagag aaactaaaaa caggaacaat gcattgaaag aacaactatt gagggatatc
    22681 aaacaggata tcaaaccata aaaggcctac aagttaggcc ttttctttat atgaaaagga
    22741 gaaatcaatt atggataaat atataaacat gaaaaaccct ccaagattta gtggagaatg
    22801 gaagtttttc gcttttttct ttttagatct attaacgctt ggtggctgct ttttttgggc
    22861 gaaaatggtc aataatcttg tccacttacc actctttttt gcactcttga attatggctt
    22921 atggattgcg atagcgttag tgattgtttg gcgaccctat aataacccag gtcaacgaca
    22981 aatttttgcg attatcgagt ctattttttt tgcggacaag aaccattatc atgcgttaga
    23041 tcctaaccga aattatcatg gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca
    23101 aaattcagtt gaaaggaaat ccattttgca gtttggaaaa ccagaagagc gctctatctt
    23161 ggacttattt ccgtataaat cctgtacaga agagggagtc ttaattacta gagaagatca
    23221 ttttcaacgt tattatcgtg tagtttctac agacgtggaa ggattaaatg aacaagagaa
    23281 actagaacga atgaatcagt tgaccaccgt catgcgtacg tatgtgccaa caattaagtt
    23341 agttagtttg accacagaga cggatctaac ggaacaaatc gtgcaaaagc ggcagttatt
    23401 agataaaaat cgtatggaac agtccttagg aagaaatcta cagcgattaa gaaaatatga
    23461 aaagaaatta gtagaggaaa tccaggaatt gaaacgagca gaacaggaac gccctgattt
    23521 aagcttcttt ttcgtcattg aaggaaagac aatcaaggat ttgcaaacca gaaaccgtca
    23581 gttaatgcga tctagtggca ttttagggtt aaaaccactc tacaaaaaag aattgatcaa
    23641 gattctttat cgaatgaata atatgaatga tgagtaggaa tggggaagag agaatgaaat
    23701 atccgttgat atggttagaa aaagatgttc cgaaagaaga gaccactaga aaatttcctc
    23761 acactttaag cctgggtaag ttgggaaaag gaaaatcttt taaccaagta aagaatcaaa
    23821 caagaagagg tgactgtaca tgttagaagc aattaaaaca gctttttcac cacaaaatga
    23881 tcaaaaggta cttgatccat taaagcaact aggaaatcgg gaaatagaaa ttttaaaatc
    23941 caaagggtat gatttagact ttttaaaaca agtgatgcca caaggtggat taaatttcga
    24001 tgaagagtat gtgacctatg gaaatggtta ttcacgatgt gtgacagttt atcactacac
    24061 aaaaaatcct acgctttttt ggttggccaa tctcatgaac aatcctcata cagtggcaac
    24121 actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaatc gtgccttaga
    24181 tgaattaaat gatcggtcag ataatgaacg aaaagcgact gaccgaaata aaagcgcgga
    24241 agaataccta gaactagcgg aatatgcccg aaaattgact tctaatgggg aaatttctaa
    24301 acaagtcaaa gtgagagtac atgtttacgg aaacacgatt caagaagtag atgaacgagc
    24361 acaggaactg atcgaaaatc tcaaagggat ggattatcgt gcagtgacct atctgcacaa
    24421 aacgaaaaga gactggcaat cgatgttcac catgttcgat gaacaagctg aatggtttgg
    24481 aaatatcaaa gggcaatcac taccatcgct aaacattggc ggaggtttcc cgttccacca
    24541 ccaaacgcta aaagatccag gtggaacaca atatggggaa accatgacag gcggcccgtt
    24601 tgtttggaat gccacgtata aaaatagtgt gcggttgtct accaatacct tgcttctagg
    24661 aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc atttagctgc
    24721 aggagacttt atttggggct ttgaaaaggg aaaagacttt attccctttt taaaggaata
    24781 taacggcatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt tagaaatatt
    24841 tgccacacgt acgtatgatg aagcttccag tttatatgac gatggatcag tcaaagattt
    24901 gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtggtct atcaggtaca
    24961 gttagtgagt cctcagctaa aaggaacgat gaaagcagaa tttaagacct atttaaatcg
    25021 tttttatgaa gagtatggaa cagtaccacg cggctttact tcctcaaatt cgcgttcaaa
    25081 tactgatacg caagtcacag gtaaagatcc agaagcttat ccgacgttca aagaattttt
    25141 agacttttta ggacaactgg aattaccagg tgcttcccaa gagaaaaaga atcgaaaaga
    25201 agaaatggaa tccatcgtag aatccttatg tgagacgtat ggcatgattt ttgatggtca
    25261 tagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg actctattac
    25321 aggcttagat cgaggggtac aacaatgtca aatgtacatg gctatgacgt tgatttggaa
    25381 tcaggcattg attcaaggaa gaagacaacg acgtttaatt aaagaaaaac aaatggaaga
    25441 acaagacttc aaatgcttct tggcgatgat tgatgagtgc cataatggtg cgacctgttc
    25501 tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta ttgtaataac tcttataacg
    25561 agaagtcaaa tgagtgggta acgccacaaa gggcttccac tgatactccg acatgcaaca
    25621 agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa gtcggctgaa ggataccgta
    25681 aggtaaaaga aatagtgaat accacgaaag ttctctagag gtaaagggtg tatccgataa
    25741 gtcgggtgtc caaaaaaact taatatggtg agtatgtaca aacgtactga cgaactagcg
    25801 aatgtactgt ccgaaaggca tagctcctag aaatgggaga gacagtgagc aaaaaatcac
    25861 tcattgtgct aacttgtggt tgagtaaaag tcgttgttat gaaagaccat gctgcgttac
    25921 aggcgtagga gaagttaggg ggcaaaagct agcatctaag ttaaggcatg tggatagata
    25981 taagggaaca ggggaagcca tcaacatgga gagtgcatac tcgatgaagt ggtagtaaga
    26041 aatatgtgaa tgacaaataa cttactttag tgatggtgaa agtagagtca cgactgatga
    26101 aggttctgta atggaactgg aggaacaggc tcgattcgtc tgtaaaagtc atgaaacccg
    26161 ttcaaccatg ggattctcgt atgactaaaa aggtcacgag ccaattggag cgtgataaac
    26221 catgacaaaa gaaacaagtc caaaagatag gaagttgcgg catgcagaat attatggcat
    26281 gacggaaatc ttcgataaga tatactccaa aagtttgaag ggagataatt tcaagaatct
    26341 aatgcagatt atcatttctg ataacaacat tgtcctagca tatcgtaata tcaagagaaa
    26401 tcacggtagt gtgacaagag gagaagatgg agtaacgatt aaagatattg aaaatctcaa
    26461 ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca cattataacc ctcggaaagt
    26521 aaggcgtgta gaaattccta aatataatgg taaattaaga ccgttgggta ttccatcaat
    26581 atgggataga atcgcgcagc aatgcatctt acaagtatta gaaccaatat gtgaagcaaa
    26641 attcaataat cacagctatg gtttccgccc aaatagatca gcagaacacg caatagcaga
    26701 ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg gtagatgtag atatcaaagg
    26761 tttcttcgac gaagtcaatc atactaaact catgagacaa atctggtcta tgggtatacg
    26821 agacaaacag cttctcttaa tcattaggaa aatgttaaga gcacccgtag tacttccaaa
    26881 tgggaagttg atatacccaa caaaaggaac accacaaggc ggaatactta gcccattact
    26941 tgcgaacatc aatctcaacg aatttgactg gtggatagca aatcagtggg aggaaaaaga
    27001 tgccaaagag ctaaaacatc attttaggaa aaatggagct tgggataaag gtaatatcta
    27061 tcacaagctc agaaagagta caactctaaa agaaatatac ctagtgagat acgcagatga
    27121 ttttaaaatt ttctgtagaa attataatga agcgaaaaaa gttttctatg caactaaaat
    27181 gtggttacag gagcgtctaa agttaccaat atctgaagaa aagtcaaaaa tcacaaatct
    27241 ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa ttagtgacca aaggtaacaa
    27301 aaaggttgtt aatacgcata taggtacaaa agcacaaagc atgattaaag tgaaactaaa
    27361 aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa gataccttta tctatattgg
    27421 agtctataac agtatggtta ttgggattca taattacttc aaaattgcga ctcattgtag
    27481 tgtagcaatg cgaaaaattg caagagaact cagaccaagt tttgaaatac gactacgaga
    27541 acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa ggatacaagc cttacctacg
    27601 gtcaggacaa atacgatata taagagggtt tccaatatta ccagtagggt atattcaaca
    27661 cagacatccg atgtgtaaaa aaagaaatat taataaatac accgaagaag gtagggaagc
    27721 cattcataag aagcaacaaa ccgtagaaga atggaagata tcgtggttgt gtagcaatcc
    27781 agtgcttagt aagagagcaa cgattgaatt tgcggacaat cgaatttcaa aattcatcgc
    27841 tcaaaaggga agatgtgcag taacaggtga ggagcttatc ttatctgaaa tgcattgtca
    27901 tcatattatc ccttatcatg aaagcaaaag tgatagctat gaaaacctag tgattgttac
    27961 tgaggaggtt catcgggtaa tacacgctac acaaagtgaa acgattgagg aacttctaaa
    28021 atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat gagttgagat taaaagttgg
    28081 taacgaagaa atatcttaat aaatatttgt aggacgggaa aacagaatgt cttttataga
    28141 cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg tggactgggg gaaaagacgg
    28201 agataatatc aaactcttac ctatcagtat cgattaatcc agattttatt gaaacggtaa
    28261 attatgtcac aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta
    28321 cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga
    28381 agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac
    28441 gtcatataga aggattagtt ggtaaatcaa tgacaaactc agaattagaa tccattcctc
    28501 aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt
    28561 ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa
    28621 tgaaagttta atcaagcagt atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt
    28681 gtttggtact tctgcaggtt tgattactat tttgattact gtgttcgtga ccctttttct
    28741 aatacttgcg gcgggttcga ttgataattc tgattcagat tcaagtagtg gtggcgaagc
    28801 ttttacagga gaatattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc
    28861 tttttctgat gagattgcgc aatatgctgt aggtgcagca gtgaaatata aattattacc
    28921 aagtgtgatc ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag
    28981 aaatgatctc aactattttg ggattacttg gtttgatggt tgtctgtttc ctaaaggaac
    29041 tgcgcgagga atcggtggta ttgaaggtgg ctggtacatg aagtttccca actcaaaagc
    29101 agccttctcc tattacggct ttatggtagc cacacaatcc aattttaatg catgtgtagg
    29161 aaataaaagc cctggcgcaa gcttattgat cttaggacga ggtggttatg ccgctgcagg
    29221 aattactgaa gatagtccgt attatacagg ttgtatgtcc attatcactt ctaataaatt
    29281 aaccgaatat gatgaattcg ccatcaaaca ttggggtgaa ggtggcaata acaacggcac
    29341 gatcactggt gaatggacga atccattccc tggcagcagt ttagataaaa gttccttcag
    29401 tggtggtcaa ttatttggaa caaatccagg cggtgaattc cgtccgaatg gcttccatga
    29461 tggattagac tttggttcag tcgatcatcc aggaagtgaa attcacgcgg tgcatggagg
    29521 aaaagtcgtg tacgttggca atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat
    29581 taattacgac ggtttgaata tggtatatca agaatttgcg aatagtacag gaaattctcg
    29641 tgtgaaggtt ggcgatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca
    29701 cttgcattta ggtttcactc gaatggattg gcgtcaagca caaggacacg cattcacaga
    29761 cgatggtaca tggattgatc cattaccatc tttgaatagc agcaaaaaat agatcaggag
    29821 aaaaaatatg gaaaataaaa ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt
    29881 aatcggcaat atcgcgttgt tttggcagct gaaagaagaa caagagacaa atacctttaa
    29941 aattgaagaa gtggctaaaa acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca
    30001 ggcgaagatc gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct
    30061 gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc
    30121 agaagagttc ggtcaattgg ccattgataa atcattaagt agaaatgaat taacggaaaa
    30181 actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag acgatgatca
    30241 ccaacatgat gattacggaa gttactcctt tacattaggt gacgtgcaaa cttatgctca
    30301 aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa
    30361 tccgcaattc aaagatgtca aaccacaaaa aataaaaggt ggattaactg tcactgaaaa
    30421 acaagtagat ggtcaatgga aagtcataga ctttagttat ttcactcgtt aggaggaaga
    30481 aaatgagtaa gaaaaaagga gaaagacaac gtttcaaaaa acgaaaaagt tatcttcaat
    30541 ggctgggaat ctttatgatt ctcggctttt tgattcttgc tagtccgatt tatacgaatc
    30601 agaaggtaga tataccagat gaggtaagca cgaacgcctt tttaaacacg aaagagctga
    30661 caatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcgatcgatg
    30721 atgaagatca aagcttgtta aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga
    30781 cagctaaagg ggattatcaa gcaatacgag ccaaagtgat caaggtgtct gatgattatt
    30841 ttgcagtgaa gctaacgcat gtaccagaag aatatatcgc catgcgttta accattattc
    30901 ctgaaaaaat tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgtgc
    30961 acgaagataa ggttaaagat caagtgaaag atgaagatta tgaacaacac gcgatcaatt
    31021 acaaagtgaa aggatacaag aaagaacaaa aagcagctca aaaacaaatg gaatctttcc
    31081 aagcaaccat tgatttgaat gaagaattag tgaagaagct aaaagatcaa ttgccttatc
    31141 aagtcgcaga tgatcaggaa aatactgaaa acaagattaa tagctaccag caagaaattg
    31201 aaacatcgaa aacgcaactg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa
    31261 ttgatcgtat gacaaaagaa aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa
    31321 caaacttggc tgacaaagat actttcgctg agcagaaagt attgattaaa gcctatctca
    31381 aagttgtaaa agctaaagaa atatgtcgag aagtgaattt atctaacaca acaatatctg
    31441 agttgaagaa tggtcgaaag aatttagata ctttaaaatt cagatcattt gaagctctat
    31501 atgtagctgc taaaaaatat tttacagctg ataacaaata aaaaacagga gtattttatg
    31561 tgaaaaaagt atataaaaaa tgcttatgaa tcagctatat caccttaacc atttgttaat
    31621 gagatgggag aaaacattaa ccacgattct aacgcaaaca tcaagcacat aaaacattta
    31681 acactcctcc tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag
    31741 catacacttt ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa
    31801 gttagaagga ttttttattg tctaaataca caaataacct taatttttaa agagcataag
    31861 gaaacgtgat atcactttct cacgaaaggt gttaggtagg ctgtgcctac cacgtttcta
    31921 agaaagataa atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag
    31981 cgaatgtggg cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata
    32041 tgtgtagcta cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat
    32101 tagaaatatt gaagaaaagt atattaataa aattgacaaa cgttgtgaag aacttacggt
    32161 caagactggc aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt
    32221 tgatcacgct cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt
    32281 ggatattcaa acttataata cgaaaacttt agaagaatat attgcaacaa ataatcaatt
    32341 aattggtttg ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca
    32401 attttggcaa aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt
    32461 gatgaaataa tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg
    32521 tgtatgatgt atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat
    32581 gcaatgggaa aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatcttcat
    32641 ttgaaagata caggaatact aattggtaca aattcaactg tttatcaaga agcagaagaa
    32701 cgtgtggaag gaaaaattaa atccaatcaa accaaaaaat attcttctaa gttaaagaaa
    32761 ccagtgaatg aatctgttca taccagagta gatccatttg atccaacgaa gattctaaaa
    32821 agataaaagt agaaggagaa cgtgatgaat aaatcgcctg cagttattct tacttgtcaa
    32881 tttaccttgc ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa
    32941 gcactggaag aaatggaacg acgtacgccc gaagaagaac aggaactgca acgaatcaag
    33001 aatgctttag aggattttta tattcctgaa ggagaaactt tttcggaaag taagacggat
    33061 aaagaattca gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt
    33121 gacaagttag aggaacaaga ttttaccaaa tatctttctt atatgacccg tcactatgcg
    33181 ttagaacaaa agaaagaact gacgcaaagg gaacaaaaag aataccaacg gttgaaccaa
    33241 gcaatgaaaa aatatcaaac agagccacat aaaaaagctt caggaaatct tcctggagtt
    33301 ttttctatgg cttcagacga agtgaaagga agtgatctga aggagatcaa acaaatcttc
    33361 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttatctctca cgacaattcg
    33421 tatcttgaaa aaatgggctt gtacgatccc aaaacagata ccttagatga agaaggtttg
    33481 aaagcagctg gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca
    33541 ggttactgga tggcaacgat tcaccgtaac actaagcata tccatattca ttttgcagtt
    33601 gttgaaaaag aaaatacgcg taaaccgtat actgagattg aaaatggtgt gagttatatc
    33661 gtaccgaagg ggaagagaag acaagcaaca ttagacgcaa tgaaaaccaa ttatgtacat
    33721 gagttagaac gtttctctta tgaaaaggat cgaatgatcg gtatggagaa acgaaattta
    33781 ttgacacgaa aaagtgattt gcgtaacacc ttaacaaaag tagtaaaaga accagcacgt
    33841 tacgatcgac acgccatgaa cttattaaat gaagtatata aaaccctacc tgaaaaacgt
    33901 tcggaatgga attatggaga tgaaaatcga accaagcttt caccatttac aaggggaaaa
    33961 cttgatgagt taaccaaata tgttttaaca aaagaacctg gatatcaaga atatatacag
    34021 atttcaaaag cattgaaaga agaagccaaa agcctttatg gggaaagcaa aagagaaaca
    34081 aagaatgcag aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgcgatttta
    34141 acagaactga agagacagga tagtcaaata aaaccattaa tagaaagtgt caatcagatg
    34201 caatctattt acaatgaacg atcctttcga acactcaaac agttgccaac aaatgaatac
    34261 aagtatagtt atcaagaatt cgtaaagcgt aggaaacaat tccaacaaag agttgtcacg
    34321 cagcgaagct tgcgtcaatt aaatcgaaca attaatgcac atgaagaaaa ataccacgca
    34381 agtaaagcat atgaagagca acagcgaaga atccaaatgg aacaagaaag acaataggag
    34441 gaagagaaat gaaacaatat ttagatcaat ggaaagtcat agaaggatca ctaaaagaag
    34501 aacgaatcga acaacttcct gattgtttag aaaaagagca tttgtttcag ataagcgaaa
    34561 tgttgagaaa cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg
    34621 gtgtgtattg ttgtaatcat gtgaagggtg aaaaatattt tattattcaa gaatacgaag
    34681 gtaagttagc gccttattat actacttggg aaataaatga agagggaatc aataatttcc
    34741 catgtgaaag cattgaagag tctatttctc tcacggaatg ttaaataaat agagacgtaa
    34801 aagagtcctg aacgtgattg tccaggactc tttttcaatg aattgttttc cgccaattca
    34861 ctgagtggtg cgattcaatt ttattatatc aaaagaaagt gagattccaa agtgaaggga
    34921 ggaagaagaa atgactcaga caacgattgg tgttgtggtt gaaaaaagca gggataatct
    34981 cgtttttgtg acagaaattc aaacaggtag agcttttgta gtcacagaca aagctgcaaa
    35041 agcgtatcaa aatggcaata ttttaacgtt aaatctgact acgaagatgt tcgttgatgc
    35101 agtagaaaat tatccatttg tttagacaaa cgatatttta c
//
LOCUS       DACDID010000012        34709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDID010000012 DACDID010000000
VERSION     DACDID010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.012"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV236_000471"
     CDS             156..1862
                     /locus_tag="IV236_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8770692.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV236_000472"
     CDS             complement(2020..2922)
                     /locus_tag="IV236_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8770693.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV236_000473"
     CDS             complement(2919..3623)
                     /locus_tag="IV236_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8770694.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV236_000474"
     CDS             complement(3654..4295)
                     /locus_tag="IV236_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8770695.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="IV236_000475"
     CDS             complement(4333..4602)
                     /locus_tag="IV236_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770696.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="IV236_000476"
     CDS             complement(4575..5264)
                     /locus_tag="IV236_000476"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8770697.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV236_000477"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV236_000477"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8770698.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IV236_000478"
     CDS             complement(5984..6916)
                     /locus_tag="IV236_000478"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8770699.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IV236_000479"
     CDS             complement(6913..7689)
                     /locus_tag="IV236_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8770700.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV236_000480"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV236_000480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8770701.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV236_000481"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV236_000481"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8770702.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IV236_000482"
     CDS             complement(12014..13294)
                     /locus_tag="IV236_000482"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8770703.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IV236_000483"
     CDS             complement(13313..14239)
                     /locus_tag="IV236_000483"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8770704.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IV236_000484"
     CDS             complement(14252..15535)
                     /locus_tag="IV236_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8770705.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV236_000485"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV236_000485"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8770706.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IV236_000486"
     CDS             complement(16323..17234)
                     /locus_tag="IV236_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8770707.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IV236_000487"
     CDS             complement(17231..17695)
                     /locus_tag="IV236_000487"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8770708.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IV236_000488"
     CDS             complement(17700..18398)
                     /locus_tag="IV236_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8770709.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IV236_000489"
     CDS             complement(18385..18771)
                     /locus_tag="IV236_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770710.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IV236_000490"
     CDS             complement(18918..20588)
                     /locus_tag="IV236_000490"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8770711.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IV236_000491"
     CDS             20771..21004
                     /locus_tag="IV236_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8770712.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IV236_000492"
     CDS             complement(21103..21534)
                     /locus_tag="IV236_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8770713.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IV236_000493"
     CDS             21613..22026
                     /locus_tag="IV236_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8770714.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IV236_000494"
     CDS             complement(22064..23212)
                     /locus_tag="IV236_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8770715.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="IV236_000495"
     CDS             complement(23334..23930)
                     /locus_tag="IV236_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8770716.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IV236_000496"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IV236_000496"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8770717.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="IV236_000497"
     CDS             27160..27591
                     /locus_tag="IV236_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770718.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="IV236_000498"
     CDS             complement(27636..28985)
                     /locus_tag="IV236_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8770719.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="IV236_000499"
     CDS             complement(29055..30218)
                     /locus_tag="IV236_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770720.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="IV236_000500"
     CDS             30347..30670
                     /locus_tag="IV236_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770721.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="IV236_000501"
     CDS             complement(30853..32202)
                     /locus_tag="IV236_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8770722.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="IV236_000502"
     CDS             complement(32271..33224)
                     /locus_tag="IV236_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8770723.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="IV236_000503"
     CDS             complement(33326..34516)
                     /locus_tag="IV236_000503"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8770724.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9863 a         7599 c         6092 g        11155 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagat
//
LOCUS       DACDID010000013        30099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDID010000013 DACDID010000000
VERSION     DACDID010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.013"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(209..694)
                     /locus_tag="IV236_000504"
     CDS             complement(209..694)
                     /locus_tag="IV236_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8770725.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1174..1815
                     /locus_tag="IV236_000505"
     CDS             1174..1815
                     /locus_tag="IV236_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8770726.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1834..2469
                     /locus_tag="IV236_000506"
     CDS             1834..2469
                     /locus_tag="IV236_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8770727.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2487..3302
                     /locus_tag="IV236_000507"
     CDS             2487..3302
                     /locus_tag="IV236_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770728.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3621..5057
                     /locus_tag="IV236_000508"
     CDS             3621..5057
                     /locus_tag="IV236_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8770729.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5246..7693
                     /locus_tag="IV236_000509"
     CDS             5246..7693
                     /locus_tag="IV236_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8770730.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7923..8990
                     /gene="rlmN"
                     /locus_tag="IV236_000510"
     CDS             7923..8990
                     /gene="rlmN"
                     /locus_tag="IV236_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8770731.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9183..10286)
                     /locus_tag="IV236_000511"
     CDS             complement(9183..10286)
                     /locus_tag="IV236_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8770732.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10839..11387
                     /gene="aac(6')"
                     /locus_tag="IV236_000512"
     CDS             10839..11387
                     /gene="aac(6')"
                     /locus_tag="IV236_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8770733.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11933..13594
                     /locus_tag="IV236_000513"
     CDS             11933..13594
                     /locus_tag="IV236_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8770734.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13664..14011)
                     /locus_tag="IV236_000514"
     CDS             complement(13664..14011)
                     /locus_tag="IV236_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8770735.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14095..15036
                     /locus_tag="IV236_000515"
     CDS             14095..15036
                     /locus_tag="IV236_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770736.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15037..16080
                     /locus_tag="IV236_000516"
     CDS             15037..16080
                     /locus_tag="IV236_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770737.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16086..17156
                     /locus_tag="IV236_000517"
     CDS             16086..17156
                     /locus_tag="IV236_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770738.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17146..18099
                     /locus_tag="IV236_000518"
     CDS             17146..18099
                     /locus_tag="IV236_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770739.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18111..19121
                     /locus_tag="IV236_000519"
     CDS             18111..19121
                     /locus_tag="IV236_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8770740.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19406..20227)
                     /locus_tag="IV236_000520"
     CDS             complement(19406..20227)
                     /locus_tag="IV236_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8770741.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20324..20602)
                     /locus_tag="IV236_000521"
     CDS             complement(20324..20602)
                     /locus_tag="IV236_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770742.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20870..22879
                     /gene="metG"
                     /locus_tag="IV236_000522"
     CDS             20870..22879
                     /gene="metG"
                     /locus_tag="IV236_000522"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8770743.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23358..23945)
                     /locus_tag="IV236_000523"
     CDS             complement(23358..23945)
                     /locus_tag="IV236_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770744.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24120..25442
                     /locus_tag="IV236_000524"
     CDS             24120..25442
                     /locus_tag="IV236_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8770745.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25597..26373
                     /locus_tag="IV236_000525"
     CDS             25597..26373
                     /locus_tag="IV236_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8770746.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26366..26935
                     /gene="rnmV"
                     /locus_tag="IV236_000526"
     CDS             26366..26935
                     /gene="rnmV"
                     /locus_tag="IV236_000526"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8770747.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26928..27812
                     /gene="rsmA"
                     /locus_tag="IV236_000527"
     CDS             26928..27812
                     /gene="rsmA"
                     /locus_tag="IV236_000527"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8770748.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28263..28892
                     /locus_tag="IV236_000528"
     CDS             28263..28892
                     /locus_tag="IV236_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770749.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29016..>30099)
                     /gene="ugpC"
                     /locus_tag="IV236_000529"
     CDS             complement(29016..>30099)
                     /gene="ugpC"
                     /locus_tag="IV236_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8770750.1"
                     /translation="YKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLRMIAG
                     LEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKYDKAD
                     IKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSNLDAK
                     LRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEVYDTP
                     VNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEVIFGI
                     RPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDFHKPG
                     EIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         9897 a         5285 c         6277 g         8640 t
ORIGIN      
        1 tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata aatatggatt
       61 tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg
      121 tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga
      181 attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta
      241 agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat
      301 ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt
      361 ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga
      421 ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta
      481 gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt
      541 ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt
      601 tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta
      661 gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta
      721 gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta
      781 aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg
      841 tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata
      901 gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct
      961 ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc
     1021 ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga ggttttttct
     1081 gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg catttgccaa
     1141 gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg
     1201 caattattag taggagcact cacaacattg aaattatttt tatttacctt gattggctca
     1261 ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac
     1321 ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt
     1381 attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt
     1441 gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg
     1501 attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg
     1561 acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt
     1621 atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt
     1681 gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc
     1741 atctatctta cattgactgg tattctaaca atcataatga aacaagttga aggaaaatta
     1801 aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa
     1861 gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca
     1921 ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc
     1981 ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag
     2041 cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat
     2101 ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa
     2161 gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat
     2221 atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc cagagcattg
     2281 gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta
     2341 cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt
     2401 gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca
     2461 attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg
     2521 caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga
     2581 ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat
     2641 ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg
     2701 aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac
     2761 tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa
     2821 agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag
     2881 aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt
     2941 catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc
     3001 aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg
     3061 tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac
     3121 tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac
     3181 gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa
     3241 caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt
     3301 aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt
     3361 tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct cttggtaaac
     3421 ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     3481 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcctttaca
     3541 taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac gattgaaggt
     3601 tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca aaagattttc
     3661 tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag
     3721 gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa ggaacgaacg
     3781 gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta atgaaagaac
     3841 aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct aatggacgag
     3901 gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa
     3961 acgatattga acctatgttg actttatatc attgggatct tccacaagca ttgcaagatg
     4021 aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat gcagaaacat
     4081 tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa caaaatatct
     4141 tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac ccgaaacgaa
     4201 tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat aaatttcacg
     4261 agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat tatccaattg
     4321 acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg gccaattatt
     4381 ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc ttagaagaaa
     4441 aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct gctaaaccag
     4501 attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca ttaaacggag
     4561 ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa actggagttc
     4621 ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg gactgggaga
     4681 ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac cgcatgccgg
     4741 tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat aaacaaatcc
     4801 atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa gaagcaattt
     4861 cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg cttagctggc
     4921 tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa acacaagaag
     4981 gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg attgaagaaa
     5041 atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga tatggccgtg
     5101 ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc tgaaaaatga
     5161 aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt tgtataatga
     5221 aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta aaaaaaggaa
     5281 aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga cgattggtgt
     5341 tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt ttttaggaat
     5401 attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc tctgtctttt
     5461 attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa ttggtaaaaa
     5521 ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt tacatgccca
     5581 tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat gggatttgtt
     5641 agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga tgattggagc
     5701 aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt tagcagcagt
     5761 gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc agctgttaaa
     5821 tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc ctgccaagca
     5881 agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag cgaaagaagc
     5941 aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg agaagaaaaa
     6001 agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga
     6061 gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa
     6121 tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga
     6181 tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa
     6241 tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag
     6301 tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt
     6361 cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt
     6421 ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct
     6481 tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg
     6541 tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca
     6601 accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt
     6661 acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg
     6721 gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga
     6781 agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc
     6841 tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt
     6901 agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc
     6961 tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt
     7021 accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga
     7081 agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat
     7141 cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc
     7201 ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa
     7261 tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa
     7321 accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt
     7381 tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga
     7441 aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt
     7501 cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa
     7561 ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg
     7621 aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga
     7681 taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca
     7741 aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat
     7801 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
     7861 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
     7921 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
     7981 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
     8041 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
     8101 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
     8161 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
     8221 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
     8281 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
     8341 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
     8401 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
     8461 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
     8521 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
     8581 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
     8641 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
     8701 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     8761 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     8821 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     8881 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     8941 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     9001 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     9061 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     9121 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     9181 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     9241 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     9301 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     9361 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     9421 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     9481 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     9541 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     9601 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     9661 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     9721 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     9781 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     9841 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     9901 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     9961 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
    10021 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
    10081 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
    10141 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
    10201 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
    10261 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
    10321 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
    10381 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
    10441 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
    10501 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
    10561 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
    10621 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
    10681 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
    10741 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    10801 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    10861 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    10921 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    10981 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    11041 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    11101 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    11161 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    11221 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    11281 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    11341 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    11401 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    11461 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    11521 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    11581 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttattcagat
    11641 aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt ttatctttgt
    11701 atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga ccatggaaaa
    11761 ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt
    11821 ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac
    11881 aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa
    11941 gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg
    12001 cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt
    12061 taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat
    12121 cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa
    12181 accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa
    12241 aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta
    12301 cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta
    12361 tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg
    12421 gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt
    12481 ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa
    12541 tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga
    12601 agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg
    12661 ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac
    12721 tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc
    12781 acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat
    12841 cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat
    12901 ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt
    12961 ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc
    13021 aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa
    13081 agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga
    13141 ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt
    13201 aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga
    13261 ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga
    13321 tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga
    13381 agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt
    13441 ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc
    13501 agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc
    13561 atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa
    13621 aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa
    13681 aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt
    13741 acataccgta gctggcagac gagagcgagg aaaattctgg ttttttcttt cggttccttg
    13801 ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata
    13861 aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa
    13921 tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag
    13981 ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt tggttcatta
    14041 tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac attagtgaat
    14101 agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct ttggttaatt
    14161 gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac gaatcaggaa
    14221 cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga taaaccagtt
    14281 atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg aatttccttc
    14341 caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc atccttgcag
    14401 ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg aacgatctct
    14461 gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat attaggtcgt
    14521 tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat caaattacaa
    14581 gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac aattgcgttg
    14641 gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt ggaagtactg
    14701 catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga aatcgccttt
    14761 aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc gttagctgta
    14821 gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg tatcggggaa
    14881 caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt aacgatcctt
    14941 tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg tctagttgat
    15001 ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc aaagcgttat
    15061 gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa
    15121 gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg gcgccgttta
    15181 aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat tttgatctct
    15241 gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc ttatatcaat
    15301 ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc aatggttggt
    15361 ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa tttcttttta
    15421 ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat gagaatctcg
    15481 ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc ttacgggttg
    15541 gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta
    15601 tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga accaggaatt
    15661 ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg tatcgttcgt
    15721 gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac attaggtgaa
    15781 tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt cattattatt
    15841 caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag ctttatcggt
    15901 ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg gtacaagaca
    15961 ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt tatcatgatt
    16021 ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat gaaagagtga
    16081 agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat acgtatgctg
    16141 gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa acattggcga
    16201 tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt
    16261 caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat atcgttggaa
    16321 aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc
    16381 caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag tcccttagaa
    16441 agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg cttaaattag
    16501 ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc tcaggaggtc
    16561 agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaatc ctgattgctg
    16621 atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag ctgctgaaag
    16681 aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt ggtgtagtag
    16741 cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa gttggaacat
    16801 cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt ggttcaatgc
    16861 cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca ccggatttat
    16921 tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg aaaatcgatg
    16981 cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca acttggctac
    17041 ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct
    17101 ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc aaataaacta
    17161 cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga agtacatgct
    17221 gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt tggagaatct
    17281 ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc tactgatggt
    17341 gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg tttgacgaaa
    17401 ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa tcctcgtatg
    17461 aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa atcatctgaa
    17521 gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc aagccacggt
    17581 actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat tgcacgagca
    17641 ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact agatgtgtcg
    17701 atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca attgacctat
    17761 ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg
    17821 cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca cggtgttcat
    17881 ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca tgagagaaaa
    17941 cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca acttcgtgaa
    18001 atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa
    18061 ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag atgctaaaag
    18121 cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt cgtacttttg
    18181 gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa
    18241 caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac ccattcttaa
    18301 aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg gcttttcgca
    18361 attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat
    18421 cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac ttaaagttac
    18481 caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga tcgattcgct
    18541 ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt tgtgatgttt
    18601 cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat ccgaagacat
    18661 tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag acagaagaaa
    18721 agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac catagaaaga
    18781 tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg gatctttatc
    18841 tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa ttggtcaaac
    18901 aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct gatttttcta
    18961 ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg tataataaaa
    19021 aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga aagagccaag
    19081 tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat gaaactgata
    19141 ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt ctaggaattt
    19201 ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc agaaaaagtt
    19261 cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga atacatcaaa
    19321 attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc ttgaatgatt
    19381 aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc tgatagattt
    19441 ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat tggagcatag
    19501 ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat ttagcaaata
    19561 cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt
    19621 gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta tggtcaaaag
    19681 ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata gccacttctc
    19741 cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt acttccttgg
    19801 catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg actgtgacga
    19861 cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca atattttctt
    19921 ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct ccagttgact
    19981 catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga tgcccttttc
    20041 tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc agtacgatat
    20101 cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac cagttaagaa
    20161 attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta gaagcaaaac
    20221 ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg tagcagactc
    20281 tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata tgaaaaataa
    20341 atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact ttataccgct
    20401 cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata aaacgcaaaa
    20461 tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa ccatacatac
    20521 gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa
    20581 aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga tagtctttat
    20641 tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga aacaaaacaa
    20701 aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg tagatagaag
    20761 tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt tttatggtat
    20821 actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg tggcagaaaa
    20881 ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc atatcggcaa
    20941 ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga tgggctttga
    21001 tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga
    21061 actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca agaaattatg
    21121 gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt accataaagc
    21181 agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact taggtgaata
    21241 tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac ttgcagaagt
    21301 gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga
    21361 attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc gtctattgga
    21421 atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg aaatgatcaa
    21481 caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt ttacttgggg
    21541 agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg cattatccaa
    21601 ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa aatactggcc
    21661 tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct attggccaat
    21721 catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg gctggctatt
    21781 gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt
    21841 ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc cgtttgggtc
    21901 agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc tggcaaatga
    21961 tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg atggactcgt
    22021 tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga cggctgctaa
    22081 cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag cattggctga
    22141 aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac catgggtatt
    22201 agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt tagctgaatc
    22261 tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag agaagatctt
    22321 caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact ttggcgaatt
    22381 tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga
    22441 agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa acgaagatac
    22501 agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt
    22561 tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc aaaaagtaga
    22621 aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag accgtcaaat
    22681 cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt
    22741 cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag gaatgatcct
    22801 ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa
    22861 tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt cataccccct
    22921 ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga agccaatcat
    22981 ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta cgagcttcgc
    23041 tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa aaaagcataa
    23101 tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta ttgtccttac
    23161 attcataaag tttgaatgca atgactttat tacatctata tttatatcta acagtagcgt
    23221 tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct cttatatgtg
    23281 tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat tgttttcttt
    23341 attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt cttcaaacgg
    23401 cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca gaaaatcgta
    23461 cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa gatcaaccct
    23521 aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc actaagtgcc
    23581 atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg ccagttcttt
    23641 gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga
    23701 aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg acctttgatc
    23761 aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa cgttgcaaca
    23821 gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg ttgttcttta
    23881 cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga acgcttaaaa
    23941 tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga
    24001 aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt
    24061 aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag aggatgggaa
    24121 tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc gttatgggaa
    24181 ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt actcagtcta
    24241 caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt gctggattga
    24301 cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa tatattgaag
    24361 ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg atttactttc
    24421 ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac ttattacaat
    24481 tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg acagggttga
    24541 atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt gttaaattaa
    24601 taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga actgtccagt
    24661 tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc agtgcgttgt
    24721 tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca ggggaaatga
    24781 aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg
    24841 tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat cttgccggta
    24901 atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt ggaaagctgg
    24961 tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca ctaactggga
    25021 tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa cagctgacaa
    25081 agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta gcagtagctt
    25141 gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt tttgtgatgt
    25201 ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa gcgccggaat
    25261 tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg attctcggtg
    25321 ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg atcggtatag
    25381 gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt
    25441 aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc ttcgtgcttt
    25501 ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc aagtagtgct
    25561 tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc tcatacccat
    25621 ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc acaagaaatg
    25681 ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa
    25741 ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac agaggctggc
    25801 agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc aaaggttgta
    25861 gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga agtccaagat
    25921 aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat cagtattcat
    25981 actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt tcgagatatc
    26041 ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt tcttgactta
    26101 gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga cgtccaagag
    26161 gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc ttatctggca
    26221 ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac agtagaaaag
    26281 attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg gacaaatgcg
    26341 caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga ttatagtcgt
    26401 tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata cgatagaaac
    26461 aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc aagagactcg
    26521 tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa aaatcattat
    26581 ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta aaccaaagaa
    26641 aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag cattgaaaaa
    26701 ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac aagacctttt
    26761 agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga
    26821 gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat
    26881 tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg agtgaatatc
    26941 gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt ttttcattta
    27001 aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa atcgtagaaa
    27061 ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc ggtgcattga
    27121 cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga
    27181 tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta catcaagata
    27241 ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag cttccgttaa
    27301 aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat tttcttgaat
    27361 cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct
    27421 ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa tattatatgg
    27481 aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt
    27541 cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca gacgaaaaag
    27601 catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt tggaataact
    27661 tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa agcttggaaa
    27721 cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa ttcgccgcat
    27781 taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa
    27841 aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catgtttttc
    27901 gtttgtttgg gcttgttacc gcagatgcaa gcttttgaac accgtttaag aaagaggttg
    27961 tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    28021 tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat gccgaacaag
    28081 gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa aaaattctta
    28141 tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc ttgacagtta
    28201 ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag gagagagaaa
    28261 aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt gggatggggg
    28321 tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt actttggcgt
    28381 ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc
    28441 taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg cgagcattgt
    28501 cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg agtatcgttt
    28561 taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat tttgtgatcg
    28621 gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt attggtaata
    28681 tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg atggggattg
    28741 gtgggcttat ccacagttta gttgattata atattgctta ttttatagca ggaacactca
    28801 gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga
    28861 ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa aaaactctca
    28921 tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa agagctgcac
    28981 caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat gacttgttct
    29041 gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac tatttcgcct
    29101 ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc agtttttgtg
    29161 taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt cactacggct
    29221 tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg aataccgaag
    29281 ataacttctt ttccttcata acctttttca actaatactt tgttttttcc ttcaggaata
    29341 cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac attaaagaag
    29401 ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt atcataaact
    29461 tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat tcggtcagcc
    29521 atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc aagacgctgg
    29581 tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa atttgataat
    29641 ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt
    29701 tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt cagacctaag
    29761 atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt acgtaatttc
    29821 aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta gttttggaaa
    29881 accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc gccgatattt
    29941 agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt tgactttcca
    30001 caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag attaaaatca
    30061 gttacagaat aattttctgc attgtcatat tttttatat
//
LOCUS       DACDID010000014        30024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDID010000014 DACDID010000000
VERSION     DACDID010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.014"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..513)
                     /locus_tag="IV236_000530"
     CDS             complement(<1..513)
                     /locus_tag="IV236_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8770751.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQV"
     gene            complement(590..1120)
                     /locus_tag="IV236_000531"
     CDS             complement(590..1120)
                     /locus_tag="IV236_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8770752.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1124..1984)
                     /gene="rlmB"
                     /locus_tag="IV236_000532"
     CDS             complement(1124..1984)
                     /gene="rlmB"
                     /locus_tag="IV236_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8770753.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1977..2372)
                     /locus_tag="IV236_000533"
     CDS             complement(1977..2372)
                     /locus_tag="IV236_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8770754.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2369..3781)
                     /locus_tag="IV236_000534"
     CDS             complement(2369..3781)
                     /locus_tag="IV236_000534"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8770755.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3778..4314)
                     /gene="cysE"
                     /locus_tag="IV236_000535"
     CDS             complement(3778..4314)
                     /gene="cysE"
                     /locus_tag="IV236_000535"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8770756.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4636..6096)
                     /locus_tag="IV236_000536"
     CDS             complement(4636..6096)
                     /locus_tag="IV236_000536"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8770757.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6266..7423)
                     /locus_tag="IV236_000537"
     CDS             complement(6266..7423)
                     /locus_tag="IV236_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8770758.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7457..8827)
                     /gene="radA"
                     /locus_tag="IV236_000538"
     CDS             complement(7457..8827)
                     /gene="radA"
                     /locus_tag="IV236_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8770759.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8890..9417)
                     /locus_tag="IV236_000539"
     CDS             complement(8890..9417)
                     /locus_tag="IV236_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8770760.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9647..10453
                     /gene="proB"
                     /locus_tag="IV236_000540"
     CDS             9647..10453
                     /gene="proB"
                     /locus_tag="IV236_000540"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8770761.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10450..11697
                     /locus_tag="IV236_000541"
     CDS             10450..11697
                     /locus_tag="IV236_000541"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8770762.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11773..12117
                     /locus_tag="IV236_000542"
     CDS             11773..12117
                     /locus_tag="IV236_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770763.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12234..12560)
                     /locus_tag="IV236_000543"
     CDS             complement(12234..12560)
                     /locus_tag="IV236_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770764.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13175..14377)
                     /locus_tag="IV236_000544"
     CDS             complement(13175..14377)
                     /locus_tag="IV236_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8770765.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14494..15009
                     /locus_tag="IV236_000545"
     CDS             14494..15009
                     /locus_tag="IV236_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8770766.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15208..15654)
                     /locus_tag="IV236_000546"
     CDS             complement(15208..15654)
                     /locus_tag="IV236_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8770767.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15701..17179)
                     /gene="msr"
                     /locus_tag="IV236_000547"
     CDS             complement(15701..17179)
                     /gene="msr"
                     /locus_tag="IV236_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8770768.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17533..18162)
                     /locus_tag="IV236_000548"
     CDS             complement(17533..18162)
                     /locus_tag="IV236_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8770769.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18146..18610)
                     /locus_tag="IV236_000549"
     CDS             complement(18146..18610)
                     /locus_tag="IV236_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8770770.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19097..20365)
                     /locus_tag="IV236_000550"
     CDS             complement(19097..20365)
                     /locus_tag="IV236_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8770771.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20596..21333)
                     /locus_tag="IV236_000551"
     CDS             complement(20596..21333)
                     /locus_tag="IV236_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8770772.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21400..21969)
                     /locus_tag="IV236_000552"
     CDS             complement(21400..21969)
                     /locus_tag="IV236_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8770773.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22097..22969
                     /locus_tag="IV236_000553"
     CDS             22097..22969
                     /locus_tag="IV236_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8770774.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23311..24183
                     /locus_tag="IV236_000554"
     CDS             23311..24183
                     /locus_tag="IV236_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8770775.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24180..26138
                     /locus_tag="IV236_000555"
     CDS             24180..26138
                     /locus_tag="IV236_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770776.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26470..27321)
                     /locus_tag="IV236_000556"
     CDS             complement(26470..27321)
                     /locus_tag="IV236_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8770777.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27444..27911
                     /locus_tag="IV236_000557"
     CDS             27444..27911
                     /locus_tag="IV236_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8770778.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28174..28656)
                     /locus_tag="IV236_000558"
     CDS             complement(28174..28656)
                     /locus_tag="IV236_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8770779.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28756..29619)
                     /locus_tag="IV236_000559"
     CDS             complement(28756..29619)
                     /locus_tag="IV236_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8770780.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8567 a         6358 c         5445 g         9654 t
ORIGIN      
        1 tacttgcttg tgatccattg ctaaacattc agccatgtct acaaaatcaa cgccttgcat
       61 ataataatga aaaattttag cttctatttt tgaaaactgg atttcgcaat tttcgattgc
      121 ttcttttaat aaaagatgct gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa
      181 gtctggtcct tctgtctcta ttttttggtc gattgagact tcatcttttt gatcacgacg
      241 cttcagcgcg ttctgcttcc tcaacagaga atagatttga ttgcttataa cgcttttata
      301 ataacttcca aagctaacac ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc
      361 gatccttgct tcttgttccc aatcctcaga aacataattt cttaaataat aggtgttttt
      421 caaacgtagt aaaagtggaa aatatttttg atacagggca gcaaatgctt cttcgtcacc
      481 attacgtgca gcgcgcaata actcctcaga cataaaataa ttcctccata aattcgttta
      541 tggaggaatc ttatcatcaa tgattcatcg aactgattag aacgatatgt tatttatttt
      601 ttctcattaa atcgtccaat ttttgggaca atttttttaa ctgctcttca ttccatggag
      661 aattacgtcg gaaatcaata aaatgcatat ctttattttc ttgggctatt gttttttccg
      721 ttttttttat cgtcttatat aattcataag cagaagttcg taatgctcct tgggaaaaga
      781 tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca
      841 gctgaccagc gattctctcg atataactgt cagccgtttc gtcttctttc gtaaaaacaa
      901 catccaactg gtatttctta taattttgtt ggatgcccgg aactagttgg gcatcgaaga
      961 caacgatgac ttccattcct tcatatttcg cataatttga taaacgatgt aaaagagctt
     1021 ctcttgcatc cgccattttg tcctgctttt taagctggac aagttccggc cacgaaccaa
     1081 tcatgttgta gccgtctaca atcaacagct gttttttcat ggtctactcc ccctgacgtt
     1141 gggaaaaagc ctgatacata agcaaaccag cagcaacact ggcatttaag ctctggacgt
     1201 gcccggtcat cgggatcgtc agcaattcat ccatttcttt tttcaatcct ggactcattc
     1261 ctttgccttc attaccaatg actaaagcaa ttgatccttt ggcattccat ttttggaagt
     1321 ttgtgccggt catatctgtg ccaaataccc aaaatcctgt ttctttgagc tgttgcacag
     1381 cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc gactgcccca gtggaagtct
     1441 ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc
     1501 cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg tggatcttcg atgctatcca
     1561 aaatcaaaaa gaaggggtct tcttttttct cttttgttcg ttcaatcagt tggtcaaggg
     1621 ataaatattc ataaggtgta atcgcaagaa cgactccttg atgcacgccg tgatcgctca
     1681 aagtttctaa tttttgttta ggaacccatt tgactggcac agcttgttca cgtgcgattt
     1741 ctttcagttc ttctactttt tctcctcgtg aatcttcttg aagaaagagt ttattccctc
     1801 gcccttgttg tagcgcttct actgcggcat ggtgcccaaa gacgaaatta tcttctaatg
     1861 cttcttcatt ttgatcagaa actgactgat ttcgtccagt ttgcttttta tttgaatatc
     1921 tattttgttt tttgtcttca aatccacgtt tcgattgatg tttgtttgtt ctttctttac
     1981 ccattttttt caccaacttt ctgaatacac cagcggatca attcttccat ccgctcggtt
     2041 tgttttgtca aatgcagata gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc
     2101 gtaacatcag cattttttgc gctcgtatgg cttttagcat ttcttcctct gcgataataa
     2161 agttcttctt cttctgtcaa aagatgttct tccagcattt gctgaatcaa aaaggcttgt
     2221 gcattcgcag aaacataatt cgttgccgca cgatgcagct tattgggttt tgtttctcct
     2281 tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg catctccaac ataagccaag
     2341 gctaaaccgt tcaattgtgt gtaatctctc atgcttctct tctccatcgg gttccttgcg
     2401 gtgtatcttc taataagatc cctttttctt tcaacaaatc acgaatttcg tcgcttcgga
     2461 caaaatcacg attgcttctt gcttgatttc gttcttcgat caattgttca atctcttcat
     2521 ctaataactc ttctgctgta aataaaatac cgaagatcga aagccattgc gagaataaag
     2581 cttctccttg tgccaagaca gcggcagaaa ctttcggctg gtctagataa gtgttcagcc
     2641 acttagcaag ctcatagacc actgtgatcc catttgctgc attaaaatca tcgtccattt
     2701 cttcgatgaa tcgctgttct aaattttcta acaaagcgat tttatccgca tcatctgcca
     2761 aattctctga ggcagtttct tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt
     2821 gtaaattggt ggctgcttct ttcatcgtcg tttcactgta gcggatcgga cgacggtatt
     2881 gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac gtttttgatc atttcatgta
     2941 cagtgatgaa attccctaga gatttactca ttttttcatc gtcttcgcca atcgttacat
     3001 agccattgtg catccagtaa ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga
     3061 tttcattttc atgatgagga aattctaaat cttgcccgcc accatggata tcgatcgttt
     3121 ctcctaaatg ttttgttgcc ataacggaac attcaatatg ccaaccggga cgtcctttgc
     3181 cccatggtga atcccaagaa atctcgccag gttttgcttg tttccataaa gcgaaatcta
     3241 atgggtcttc tttttgctgc tgttcttccc ctgtacgctg acttgctccg acttccagtt
     3301 catcgatgga ttggtcactt aatttcccat aattttcaaa tttacgggta cggtaataca
     3361 catcacctga agattcataa gcatagcctt tttcaaccaa cacttgaata aatgctaaaa
     3421 tatctggcat atgatccatg actcttggat gcagtgtagc aggttcgaca ttcaacgctt
     3481 tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac ttctggtgct gtaataccta
     3541 attcgtttgc tgtacggatg attttatcgt ctacatccgt gaaattcgat acgaaatcga
     3601 ctttatagcc tcgatattca aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag
     3661 catttccgat atggatataa ttatacacag tcggtccgca gacatacata ccgacatgtc
     3721 ccttttgtat agggatgaat tcttcttttt ttctggtcaa tgtattgtag atttttatca
     3781 ttctgatctc ctcacttttt ctcctttgat tcgaacgact tttgctggta ctcctaccgc
     3841 tgtagcgtcg tctggaacat ctttcaacac aacggcacct gcaccgattt tggcgttttt
     3901 tccgatcgtt accggtccta atatctgcgc attcgcagag agcatggctc cttgtttaac
     3961 agttgggtgt cttttacccg tttcttttcc tgtgccgcct aatgttacgc catggaataa
     4021 aatcacatcg tcctcgattt ctgcggtttc gccaatgacg attcccattc catggtcgat
     4081 gaacacgcca gttccaatcg ttgctcctgg atggatctca acaccagtta ggaaacgcca
     4141 gaattgagca ttgatcttcg ccaataaaaa gagccggtgc tgatataaaa aatgagagaa
     4201 acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg
     4261 tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt
     4321 ccactccttt cgtaagaatt ttatgtagga aatcatcttc ttataaagaa aagaagcgcc
     4381 ttttaaataa gtttccttat caaaagacgc tttccgcgtg gttccacttt tcttcataat
     4441 caatgattat ctctgacagc taacgcctgt tatacgttct tggctacttt gtttcctata
     4501 aaacagtttt tcctgttttc tgtgtttcgc caagaccact cccagaggca tttcggcaac
     4561 cattcattga taccttgcac cacccggtat ctctcttgta atgatcagct gcttactttt
     4621 ttctgttcat cgtgtttagt tcagtacttg atttaaatgt gctaatgatt tttcacgacc
     4681 aagcaattcg atcgtttcag gcaattctgg accatgcatt tgtcctgata cagctacacg
     4741 gattggcatg aaaagatttt ttccttttac gccagtttct ttttgaacag ctttgatggc
     4801 agctttgaca gttggagcat ctactacttc catttgttct agctgtgctt tgaatgcgtg
     4861 gagaacagta ggaactgttt caccagcaag tacttctttt gctgcttcat tcaatacagg
     4921 atgttcattg aaaaatagcg tagacagatc aacgatttct gcagcatagc tcatttgagg
     4981 ttgatacagg ctaacgatct tcttcaccca ttcgattgtc ttttcatctg ggttttctgc
     5041 tacacgtcca tcagcgatta gataaggcag acacatgtca gtaaatgcat ctagatccat
     5101 tgctttgatg taatggttgt ttacccattg cagttttttc gcatcgaaag ctgcgggtga
     5161 tttacttagg cgttttggat cgaacatttt gatcaattct tggtgttcga agatttcgtc
     5221 ttcgcccaca ggtgaccagc ccaataatgc aataaagttg aacattgcat ctggtagata
     5281 gcctaattcg cgatattgtt cgataaattg caagattgat tcgtcacgtt tgcttagttt
     5341 tttacctgtt tcactgttga tgatcaatgt catatgtccg aaggttggtg gtgtccagcc
     5401 aaatgcttca tagatcatca gttgttttgg tgtattggca atatggtcat ctccacgtaa
     5461 aacgtgagtg attttcatca aatgatcatc gaccgcaaca gcaaaattat aagttggcat
     5521 accatcgcgt ttttggatga caaagtcgcc accgatatta tcggattcga acgaaatagc
     5581 gcctttcacc atatctgtaa atgcgtattc tgtattgcga ggcacacgga aacgtacaac
     5641 agattctaga ccttgtgctt ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt
     5701 tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct tcccgttctg cttctaattc
     5761 ttcttctgtg caatagcatt tgtatgctcg attgctagca agcagctgat caatcagcgg
     5821 ttgatagatt tctttacgtt cagattgacg gtaagggccg tattctccag gattttcagg
     5881 agattcatcc caattcattc ccaaccaagc aaggttttcc aactggcttt tttcgccatc
     5941 ttcgatatta cgtttttggt cagtatcctc aatacgaata ataaagtctc catcattgtg
     6001 gcgggcaaac aaataattga ataatgcagt tcttgcattt ccaatatgca ggtgcccagt
     6061 tggacttggt gcatagcgta cgcgtacttt cgtcattttc ttcctcttct ttcaaactag
     6121 actttgacta acagcaaaaa ttccgtcagt tacggcttct caaatatcat ttatttaaag
     6181 ccactcagca caaattttac cttttctaag gtaaatagta aagccaactg gttgaatttc
     6241 cttaccagtt ggctgaattt cttttttatt ttgattgttt agtctttttc tctgtctctt
     6301 ttttattgtc gattcctcgt cctgcatgtg caggtttagc aaagatcatg cgtccagcag
     6361 ctgtttgaag ggcacttgtt acaacgactt cgatacgttc attcatgtaa tgctgtccat
     6421 cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc
     6481 cggcttttac tactagaaca ttcatcgttt caccagggat gacgactggt tttacggcat
     6541 ttgccaacgc attgatatta agaacaggta cattttggaa ttcagagact ttattcaagt
     6601 tatagtcatt ggttacaatg acaccatcta aaagcttagc caatttgatc aatttgctgt
     6661 ctacttcact gatatcttca aagtctccat cgtacatttc gacagatata ccctcttctt
     6721 tttgcaaggc atttaggata tctaatccgc gacgaccacg cacacgtttc aaactatccc
     6781 ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt agggatcatc aaggttcctt
     6841 ctaagaatcc agtctttgta atatcataaa tccgaccgtc aatgatcacg cttgtatcca
     6901 agattttata tttatggaaa ttttctcctg attttcgttc taacagttga ctctcaactt
     6961 gactagctgt ttcttgctcg atttttttgc ttcgtgaacc aaaaactttc ttccattctt
     7021 cgattcgtgt cgttcccatt ctcaatccca gatagcctaa gatgatcatc agtaaaatcg
     7081 gcaatacact attgacgaat ggaatttcca tattgtaaag aggggtcgag atcacaacac
     7141 ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag aagataagtc aagctgacct
     7201 cacttagttt ttgttctact tgtttgataa tagctgagat atgtttggcc aacagcaaag
     7261 ataataaaaa gaaaataagt gcgcctaaca ggctgttggt aaatgtatta ttcagccatc
     7321 cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa aaatgaaata cccaagcttg
     7381 cgccgacaat gatcatcagc aaagtaatga cacgtttttg catcgttttt cctccgtttc
     7441 gacaatcatt tattagttag cggaacacac gcttcaacgt ttcagccaaa gttgctactc
     7501 caactatctc gattcctttt ggcgcttccc agttacctaa gttgttttta ggtaggtata
     7561 cttttgtgaa gccgagtttt tggacttctt tgactcgttg ttcaatatga ctgacacgtc
     7621 tgatttctcc agtcagtcca atttcaccga tgaaacattc cgttggctgt gttccttttt
     7681 ctttatagct tgaagcaata ctgacagcga tagctaaatc aatcgctggt tcatttaatt
     7741 ttaccccacc tgcagctttt aagtaagcat cttgattctg aagaagcaac ccagctcttt
     7801 tttccagcac tgccatgatc agagataccc gattgaaatc aagacctgtt gttgttcgtt
     7861 tggcatttcc gaacatagta ggtgtgacta atgcttggat ctctaccaaa attggacgtg
     7921 tcccttccat tgccacaacg atagcagagc cagttgcacc gtctaggcgt tcttctaaga
     7981 aaatctgtga aggattagcg acttcttcca atccatgctc atgcatttca aaaatcccaa
     8041 tctcattcgt tgagccaaat cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt
     8101 gttcaccttc aaaatataac accgtatcca ccatatgttc taacattctt gggccggcga
     8161 tcgagccttc tttggtaaca tgaccgacga taaaaatggc tatcccattt gttttagcgg
     8221 ttttgagtag ttctgcagtg gtttctctta cttggctgac acttcctgcc acacttgtga
     8281 tgtctggctg cgtcatcgtt tggatggaat caatgataac ataatcaggg gagatatgtt
     8341 cgatggcacg actgatttcg ttcatatctg tttcagcgta tagataaaat tctgtgttga
     8401 tacttccgag gcgttccgcg cgtaatttta tctgctctgc gctttcttcg ccggatacat
     8461 ataagacttt tccgcctatg gctgctaatt gctgagaaac ttgtaacagc aaagtggatt
     8521 tcccgatacc tggatcgcca ccaatcaaga ccatagaccc tggcaccaca cctccgccta
     8581 acacacggtt cagttcttct aattttgttt tgatccgagg ttctttttta gggacgacgt
     8641 ctgcgatttt tgttggtttt gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg
     8701 ttgtatcttg ttcgatttct tcgaccatcg tattccattt tccgcaattt gggcagcgtc
     8761 ccaagaactt aggcgacaca tacccgcaat tttggcaaac aaattgtact tttgattttt
     8821 ttgccattgt accgcctcct tttatcctaa tcatgagaca ttaaatcaag cttaagtatc
     8881 tttaacgttt tatttactgg aagaaccaaa tccgccttga cgttgagcag tcgtttgatc
     8941 ttgatctgct tttaaaaacg gcaaaaagat cccttggccg atacgctcgc cttttttgat
     9001 cactacatcc ttgaaaccaa agttggtgaa ttggaacatg atatggcctt catttgcctc
     9061 attattgtaa taatcactgt caatcacacc tactccgttt gtcaacacta aaaaccgctt
     9121 caacggattg cttgaacggt tagcaagctg caaaaattca tcttctccca tgtaagcttt
     9181 gattccagta ggcacgaggg ttggtttcaa ttgtttttca ttttctgata catctttttt
     9241 caatccatct gcgactgttt ccaacccctt tttccagcct gctttccaga tacttggaat
     9301 cacgacctct tctgctgctt caaaatcata gccagcagca tgatgtgtag cgcgctgcgg
     9361 tagattgata ttttgatctt tatatttaga aataacttca aatcctcttt ttctcaccgg
     9421 ttttcctccc taaataaact actcctattc taacatatcc gaggctgtaa aagactttct
     9481 ttacaaattc ttcaagaaat tctgctatct taaagtaaac cattaattcg ttctttttcc
     9541 aaacgactgc aagttgtaca aaaaaactat tttttacatg actttactcg tcaaaacaag
     9601 tagaatagaa cgaaattaat gagtggatat ttgattgagg tgtcacatgc gtaaagaaat
     9661 taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt acattgattt atcccaatgg
     9721 aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg
     9781 gggaaaggaa gtgatcttag tctcatcagg agctgtaggc gttggtatgc ataaattaca
     9841 tttgtcagaa cgtccgactt tgattcctga acagcaagct gcggcggctg ttggtcaagc
     9901 cgaactgatg aatatctata atcaaagatt cagtgtatat aatcaacaga cggcacagat
     9961 cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt
    10021 ggaacagctt ttgattatgg acacgatccc tatcgtgaac gaaaacgata ctgtagcagt
    10081 ggatgaatta gatcatctga caaaatttgg cgataatgat cagctgtcag ccatcatggc
    10141 aaaacttgtc gcggcagacc ttttgatcgt cctttctgat atcgacgggt tttattcaga
    10201 taatccgact acaaatcccg aggctcagat gtatcgctat atcgataaaa tcgatgaaaa
    10261 gattctggca caagcaggtg gtgctggtag cacatttgga acaggcggta tgcagagtaa
    10321 attaaaagcc gcttctagga tatttgaagc aaacagcgca atgattttag caaatggaaa
    10381 aaatccggca attatttttg atattcttgc aggaaaagaa gtaggtactt tatttaagga
    10441 ggacttgaaa tgactgactt gatgcaatta ggacaacagg caaaagatag cgcgcaacaa
    10501 ctagcactta tgcctacaaa ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc
    10561 aaaaagaatc aacaagcgat cattgaagca aatcataaag acttagaaaa agcagtcgaa
    10621 aacaatatca gtgaaacgat gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc
    10681 atggctacag agatcgaaaa aattgcttcg ttagacgatc ctattggaaa agtagatgag
    10741 atgtggacga acaccgacgg attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc
    10801 ggcatcattt acgaatctcg tccaaatgtc acgacagatg cagctagtct agcattcaaa
    10861 tcagggaatg ccgttatcct tcgcggtggc aaagaagcat tcttctcgaa tcaattatta
    10921 gtccaattat tgcagcaagt actggtcagt gctggcgaat ctccttatgc catccagttt
    10981 gtcgatgata cttctcatga aactgcagga cagttgatgc gattgacaga atacttagat
    11041 gtgctgattc ctcgaggcgg agcaaaatta atccaacgag tcaaagaaca ggcaacggta
    11101 cctatcattg aaacaggtac aggaaataat cacgtatata tcgataaaga agcacagtta
    11161 tctatggcag tgaacatcat cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg
    11221 gcagaaacat tgctgatcca ttctgagatc gcaccttttt tcttgccagc tattgaaaaa
    11281 gaactggtag aacacggggt ctctttacgt gcggatgctc gtgcgttaga atatctagag
    11341 acggctgctt tagcagaaga cacagattgg gatactgaat atctagatta tatccttgct
    11401 gtcaaagtgg tcgattcttt ggatgaagca attgcacata tcaatcggca taatactaaa
    11461 cattcagaaa cgatcgtgac agatagttat gcggctagtc aacgtttctt aaatgaagtg
    11521 gacgcggcgg ctgtctatgt caatgcctcc actcgtttta cagacggatc ggtatttggt
    11581 tttggtgcag agattggtat ctctactcaa aagttgcatg cccgtggtcc aatggggtta
    11641 aatgaattaa cttccacaaa atatatcatt tatggcgatg gacaaatccg cgaataatgt
    11701 ttcgtgtgaa acattattcc cgccttttgc cttttttctg taaaggttct atccaactag
    11761 aaaggtgtca atatgtttga aattttaatg atttttttta tttaccttat ttctctgaat
    11821 atcgctgctt ttctgggagt cggtatttta tcccttttct ttcaattcaa aaaacgaagc
    11881 atagggagtc aacgggaaaa atggtcgcag tattttgaca aaatcgggca aaaagggctg
    11941 gttacacgct tgcacattag ttacatggtt gcattatgct tattagcgac aatcaattac
    12001 tattcttttt tcgatcattc aatcgcttat acgattacgc tactgattgc tgggattttt
    12061 cacttatctt ataaatatca gctgaataaa aaccacttga atcggacttt ccgctaaaaa
    12121 atatccgtac gataagtaga gtgccttgaa gatcggcaaa aaactgccgg actttttaga
    12181 ggctgtgaca aagattttgt cacagcctct ttttccatat aaacggcgtt cgcctaccag
    12241 ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca
    12301 tcataataac gaagcagctc atccatggac aagttcatcg ttttttcttt ttcttcttcc
    12361 gctgattcag ccagtagctg ttcccgatac acctgataca taccagctat tttttgctgc
    12421 atcgcttttc tagtttcacc ttccgcattg attattggca attctttgat atagccacga
    12481 taaatcaacc gatcatctga aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc
    12541 tgaaaatatt cttccagcat ctattatccc cccattcttt ttcttaaatg aattttagct
    12601 tacctactga aaattttcca gtttcattat tttgagaaaa aaagggaatg tgacaaaaat
    12661 cgtgtcacat cccctttttc cgaataaatg gtgttcttct gccaactcct caatcataag
    12721 tcacaatatt caacaaacat gagaaactgt tttttgaaat tgttccttgt gattcagagt
    12781 taaacgacag tctcacaacc tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta
    12841 gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt ttgtttctct aatacttagg
    12901 ataaaacgct gattttacaa cctctgttaa acggtgttca atcagctgtc cttcggtatt
    12961 agctcaaaaa acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg
    13021 acaaaacgct gattttacaa cctcctgata aacggtgttc aaaagcttgc atctgcggta
    13081 ataagcccaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttatgactt
    13141 gatgcaggac agctttttca caacctctga tttcttattc ttcttcattt gtagcaaaat
    13201 aattacggtg ataaatgata caacatacac tgataagaac aatggcacct gcaaaataaa
    13261 acgcatagct ttcaccaata tgttcaccaa tcacacctgc aataaatggt gctaaagctc
    13321 caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac gtttcgttgc agacctggta
    13381 tttccatagc aatcgtcgtc aacagtgtat tgataattcc ttggaaaaat cctgctaaca
    13441 caatgctgat cggtacaagg actggcatag aagttccagc tcctaataac agcaaacaaa
    13501 taatgaataa gacaaatgct gctaaaattg tccgataagt cgtaaacttc cgctcaagag
    13561 ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc ccagccaaaa aatacaaaac
    13621 cgacttctaa ttcagaatag ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga
    13681 atccgaaatt atataacaat gcgattagtc caactgaacg aagtttatga ttgcttaacg
    13741 ctttgacacc ttcccaaagg cctacttttc tctttggttt ctcagtttca ggtaaaagta
    13801 ccgctagggc tataaaaccg caaaacatca aaaatgctac cccaaaaaat ggataacgcc
    13861 atgaaccact gcccaagacc ccaccgacta gagggccaac agccattccg cagccaagtg
    13921 ctgcttcata catcatgatt gctttttctg tgttaccaac taaaatactg acgatcgttg
    13981 ctaaagcagt agagacaaat aaagcattcc cgacacccca gccggcacgt aacccaatca
    14041 gcattccgat attcccagat aatccgccaa gaccagcgaa tacaacgatg ataaacaatc
    14101 ctgtcatcaa tgtttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca
    14161 ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt
    14221 ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga
    14281 tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt
    14341 gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat
    14401 acatgattta ttttacatgt caatatgcac atgtaaaacg tggaaacttt actgtgatat
    14461 aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta
    14521 tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt
    14581 tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca gcagggttat
    14641 gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa
    14701 ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat
    14761 gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg
    14821 ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat
    14881 gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt
    14941 atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt
    15001 tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc
    15061 aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat
    15121 cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa
    15181 ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact
    15241 tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt
    15301 ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc
    15361 gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat
    15421 ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc
    15481 gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa
    15541 acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat
    15601 caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta
    15661 ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta
    15721 ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt
    15781 aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag
    15841 cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg
    15901 gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata
    15961 acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg
    16021 attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt
    16081 gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc
    16141 cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg
    16201 cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc
    16261 tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg
    16321 gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga
    16381 gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt
    16441 tagaggaaga caaccgatcc agtctgattt gttgcttttt ctttttactg gacacttttt
    16501 ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt
    16561 tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat
    16621 aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg
    16681 atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat
    16741 aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt
    16801 ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt
    16861 cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta
    16921 attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg
    16981 tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca
    17041 aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac
    17101 gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat
    17161 ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga
    17221 tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc
    17281 tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt
    17341 cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat
    17401 ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga
    17461 cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta
    17521 actgaaaacc ttttattcga acatagtgat cttttctggt gctaaacgaa cagactcgta
    17581 accttcttct accgcttttc ctattgataa aataataact ggaacataac ggtctttatc
    17641 caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata
    17701 gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac
    17761 tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg
    17821 ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttgt agatttcttc
    17881 gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt
    17941 ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc
    18001 gaccacaaag cgccatggtt tcatattgac agaagaaggt gcagtcgtcg cttctttgat
    18061 catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct
    18121 tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg
    18181 tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt
    18241 tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt
    18301 cacaatgctt gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt
    18361 tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag
    18421 taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt
    18481 cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa
    18541 agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt
    18601 ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa
    18661 tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat
    18721 agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc
    18781 acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag
    18841 tagataattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat
    18901 attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca
    18961 caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa
    19021 acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct
    19081 ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg
    19141 gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat
    19201 ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat
    19261 aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt
    19321 ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag
    19381 caatcccttc atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg
    19441 tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg
    19501 gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat
    19561 aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg
    19621 tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca
    19681 ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat
    19741 tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat
    19801 tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc
    19861 cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac
    19921 ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag
    19981 atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat
    20041 gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg
    20101 gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag
    20161 ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtacttt
    20221 ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg
    20281 tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc
    20341 taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa
    20401 ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt
    20461 tctttttaat aaatttgctt tttctgcata aaaagacgta aatgaagaat attcttctcc
    20521 attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt ctctctggat
    20581 attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga
    20641 catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cagatgttaa
    20701 tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg
    20761 atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg
    20821 ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac
    20881 ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga
    20941 tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt
    21001 gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat
    21061 gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat
    21121 aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc
    21181 tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt
    21241 agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat
    21301 gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc
    21361 tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg
    21421 tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac
    21481 agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc
    21541 tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg
    21601 ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac
    21661 tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac
    21721 atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac
    21781 cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc
    21841 atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tccgcttgga tctgatggat
    21901 cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag
    21961 gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt atgatgtcta
    22021 atacttataa aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa
    22081 aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga
    22141 aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca
    22201 actgaaagat ttagaagaag aacttggtgt gacacttttc actcgaggct cccgagagat
    22261 acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt
    22321 agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg
    22381 cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc
    22441 gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact
    22501 ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca
    22561 gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgga atcatccttt
    22621 agcaaagcaa acagtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg
    22681 gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc
    22741 gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt
    22801 tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaag tattcatccc
    22861 actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc
    22921 tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt
    22981 ttccttcgct ttcagctttt taacaacgaa taagttaagc aaaagatcca atgattaaat
    23041 aacataagtt tcttaatttg ttccttcctt ttatttctga taattccagt aaattactat
    23101 aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa
    23161 taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt
    23221 ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt
    23281 gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat
    23341 gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt
    23401 gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa
    23461 ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt
    23521 gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat
    23581 gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc
    23641 gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt
    23701 gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa
    23761 gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc
    23821 agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa
    23881 acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga
    23941 ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa
    24001 atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg
    24061 accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc
    24121 gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat
    24181 tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg
    24241 ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg
    24301 ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg
    24361 ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg
    24421 tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact ttattgaata
    24481 tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta
    24541 ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg
    24601 ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc
    24661 tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg
    24721 ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt
    24781 tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag
    24841 gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt
    24901 atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt
    24961 acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgtta gcagcagctt
    25021 ttgcttcatt ctttactggt gtaacagaac cacttgaatt ctcatttatg ttcgttgctt
    25081 ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct
    25141 tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga
    25201 aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga
    25261 tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg
    25321 aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg
    25381 cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata
    25441 actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa
    25501 tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg
    25561 taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcaa
    25621 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt ggaacgatcg
    25681 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
    25741 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
    25801 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
    25861 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
    25921 aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga
    25981 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
    26041 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
    26101 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
    26161 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
    26221 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agccaaacgt aacatatgaa
    26281 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt ttacaacctc
    26341 tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt caaatttatg caaaaatgtg
    26401 gaaagccatt ttcacaaaat ttttcttcat actcaaaact aatcggcttt ttcacaacct
    26461 ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag aagttaatgg
    26521 ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct ttttaatatc
    26581 atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt cttttagatt
    26641 ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tttttacctg tcgcacgctc
    26701 taatgccttg gctaattctt catagtcagt tagattgccg gataattcta agatttctgg
    26761 agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt tcaatgccca
    26821 acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag cctgcccaat
    26881 gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg aaatacctga
    26941 atccttaatc attttttcag tgaattggtg atctggcgct aatacactga tcgagtgatc
    27001 ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg ctgccttcac
    27061 tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc
    27121 tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa tacgtatatt
    27181 aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta catagatttc
    27241 attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac ctccagttgc
    27301 tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt taagatttac
    27361 agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata agtcaagcaa
    27421 aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat gcaatccatg
    27481 tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag atgattgcca
    27541 gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg
    27601 gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt ccagatcaga
    27661 tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc caagtcgatc
    27721 caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta gaagaaagct
    27781 acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt gatagtctga
    27841 tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga
    27901 gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt ctacataaat
    27961 tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc taatgctcag
    28021 gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg accctcggca
    28081 ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct ctaatgctca
    28141 ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta ccggtatcca
    28201 gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat ttggaccccc
    28261 aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat tcgtcaaatc
    28321 atttcttaat tcttcaggcg attctgctaa agcgattaca acaagatacg ttccttttgg
    28381 gaatgggatg attcgggtag tgtttggaac atcctgactc gtttgaacac cgacatagta
    28441 catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt cagcagcttg
    28501 tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt gtttctctgc
    28561 agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac ccgtcaccgt
    28621 aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt ctattatatt
    28681 aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt ttctagggat
    28741 atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac tgtaaacatt
    28801 actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta actcataatc
    28861 tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag tttggggaaa
    28921 tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca tttctaaatc
    28981 agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc
    29041 ttccaagcgg gctccatcaa atccataaga aacgttgatt acttgatttg gatataaatc
    29101 tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta acttttgtat
    29161 ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg ccacataaaa
    29221 ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat caataaaaaa
    29281 tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag gcagatatcc
    29341 tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc
    29401 cgtttctgtc actcttgatc catctaggag atcgcgatta gccaaagcca atctacggtt
    29461 tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt ttaattgata
    29521 ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg tcaaatgttc
    29581 ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc tccttgcttg
    29641 cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac caatttagtc
    29701 agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt cgtaacggat
    29761 aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt ggggaagaca
    29821 cattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat ccgcttacta
    29881 cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct tggatttttc
    29941 tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct aacaaaatca
    30001 cttcattttt gtcagataaa taaa
//
LOCUS       DACDID010000015        29607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDID010000015 DACDID010000000
VERSION     DACDID010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.015"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..283)
                     /locus_tag="IV236_000560"
     CDS             complement(<1..283)
                     /locus_tag="IV236_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770781.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVL"
     gene            complement(458..2593)
                     /locus_tag="IV236_000561"
     CDS             complement(458..2593)
                     /locus_tag="IV236_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8770782.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2676..4331)
                     /locus_tag="IV236_000562"
     CDS             complement(2676..4331)
                     /locus_tag="IV236_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8770783.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4333..4647)
                     /locus_tag="IV236_000563"
     CDS             complement(4333..4647)
                     /locus_tag="IV236_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8770784.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4817..5077
                     /locus_tag="IV236_000564"
     CDS             4817..5077
                     /locus_tag="IV236_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8770785.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5091..5405
                     /locus_tag="IV236_000565"
     CDS             5091..5405
                     /locus_tag="IV236_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8770786.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6135..6689)
                     /locus_tag="IV236_000566"
     CDS             complement(6135..6689)
                     /locus_tag="IV236_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8770787.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6815..8743)
                     /locus_tag="IV236_000567"
     CDS             complement(6815..8743)
                     /locus_tag="IV236_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8770788.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(9049..9765)
                     /locus_tag="IV236_000568"
     CDS             complement(9049..9765)
                     /locus_tag="IV236_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8770789.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9766..11589)
                     /gene="glpO"
                     /locus_tag="IV236_000569"
     CDS             complement(9766..11589)
                     /gene="glpO"
                     /locus_tag="IV236_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8770790.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11603..13099)
                     /gene="glpK"
                     /locus_tag="IV236_000570"
     CDS             complement(11603..13099)
                     /gene="glpK"
                     /locus_tag="IV236_000570"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8770791.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13235..14695)
                     /locus_tag="IV236_000571"
     CDS             complement(13235..14695)
                     /locus_tag="IV236_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8770792.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14685..15980)
                     /locus_tag="IV236_000572"
     CDS             complement(14685..15980)
                     /locus_tag="IV236_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8770793.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16312..16989)
                     /locus_tag="IV236_000573"
     CDS             complement(16312..16989)
                     /locus_tag="IV236_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8770794.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(17019..18110)
                     /locus_tag="IV236_000574"
     CDS             complement(17019..18110)
                     /locus_tag="IV236_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8770795.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18211..18645)
                     /locus_tag="IV236_000575"
     CDS             complement(18211..18645)
                     /locus_tag="IV236_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8770796.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19229..20686)
                     /gene="ascB"
                     /locus_tag="IV236_000576"
     CDS             complement(19229..20686)
                     /gene="ascB"
                     /locus_tag="IV236_000576"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8770797.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20683..22578)
                     /locus_tag="IV236_000577"
     CDS             complement(20683..22578)
                     /locus_tag="IV236_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770798.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22636..24411)
                     /locus_tag="IV236_000578"
     CDS             complement(22636..24411)
                     /locus_tag="IV236_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8770799.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24461..25957)
                     /locus_tag="IV236_000579"
     CDS             complement(24461..25957)
                     /locus_tag="IV236_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8770800.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26151..27773)
                     /locus_tag="IV236_000580"
     CDS             complement(26151..27773)
                     /locus_tag="IV236_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8770801.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28222..29535)
                     /locus_tag="IV236_000581"
     CDS             complement(28222..29535)
                     /locus_tag="IV236_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8770802.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         8491 a         6376 c         4947 g         9793 t
ORIGIN      
        1 tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga
       61 aatacagcat tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg
      121 gtctcgttaa tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa
      181 gagccactaa cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt
      241 tcaccaaaat tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt
      301 ttttgttacg acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta
      361 aatttttcct aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc
      421 tttttatcag ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga
      481 caatgctttt cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat
      541 ctccatcgtt ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac
      601 tcaaaggaat gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta
      661 gcgtttgggt ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa
      721 ccaaaatctc agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg
      781 gttcttttcc ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg
      841 ttttgaaaag ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat
      901 ggatcggcag atggtagtcg tcgaaacaac gcataatatc agcaaaagta gtggaagaat
      961 taaagattgc ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga
     1021 tcaatagacc taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa
     1081 atgctggtac atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt
     1141 catcaacagt acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc
     1201 tttcaaacaa ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc
     1261 gatatttttt ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag
     1321 aaatcaattt accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct
     1381 gcacagcagg actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct
     1441 ttttcgttgc ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt
     1501 tttcgatcgt ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat
     1561 tcggatgatc taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac
     1621 gcaaacgttc tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt
     1681 caccttctat ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg
     1741 gatttctttc acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag
     1801 gtgatagaat cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg
     1861 caatacgctg catgatagca gaggttttac cacttcctgc tacgccattt accaagatat
     1921 tttgactttt cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag
     1981 ccttcatttc attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag
     2041 ctacagacgt atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt
     2101 ctttaataac cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt
     2161 tattgtaaaa aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat
     2221 catctgtgaa atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt
     2281 ctacaacgac ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat
     2341 tttctgcgtt tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca
     2401 tcgaaaaagt atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca
     2461 tttctttcaa cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt
     2521 tttttttatc aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt
     2581 gtgtgttgac catacacatt ctcctctttt tttaaagtgg tggacccgat tagtttacct
     2641 cttttaagag tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct
     2701 tcaggtcgga cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca
     2761 gactgcttca agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata
     2821 tattcttgtt ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt
     2881 ttggcttcct tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt
     2941 tttccttttg ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct
     3001 gcaatgttca ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc
     3061 ggtgcatacg tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct
     3121 gttgctaaaa tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa
     3181 atctcgccag tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa
     3241 tagcttgcat gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg
     3301 gctgttcttt cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct
     3361 gttccaatac cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta
     3421 ccttgacgat tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt
     3481 tttacaacga tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg
     3541 tctactagga tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta
     3601 tcaggacgaa caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc
     3661 agtcgcaatc gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct
     3721 actccttgat gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga
     3781 agaatatgtg gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct
     3841 gccatttcca atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct
     3901 aatgcatgca tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct
     3961 tctgttatcc ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca
     4021 tcgtactgtt caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga
     4081 aagatggctg tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat
     4141 tctggcgttt ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc
     4201 aagccgcaat ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct
     4261 tccattaatc ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata
     4321 acgattttca tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg
     4381 tacattcact acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat
     4441 ccctgattga caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact
     4501 ttgtaatgga acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct
     4561 aacatcaagt aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga
     4621 ttttattgat tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc
     4681 tcatggggta tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt
     4741 tttcactact ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa
     4801 tagaggagaa tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac
     4861 agagggacaa attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt
     4921 catcactcaa ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc
     4981 agataatctg aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa
     5041 actagaacaa gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg
     5101 ttttccatat tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga
     5161 taaaagcttc tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt
     5221 atctagactc tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg
     5281 ccaatgctat gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg
     5341 taccagcagg tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc
     5401 cttagtcagt agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc
     5461 gaagaagcta cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa
     5521 gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta
     5581 ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac
     5641 ctaagtatta gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta
     5701 ataccgaagg tcagctgatt gaacaccgtt taaggaagag attgtgaaaa agtcgcttag
     5761 tttcgagtat taagaaaaat tttgtgaaaa tggatttcta catttttgca taaatttggc
     5821 ttaataccga gaaactggct tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt
     5881 ttgacctgag tattagagaa acaaacggaa aaatagctgc tcatattttg ccgttttttg
     5941 agctaatacc gaaggtcagc tgattgaaca ccgtttacca agaggttgtg agcctaccat
     6001 ttaactccga atcacaagaa ataatttcaa aaacaacttc tcctgttttt ggtttggttg
     6061 gcttattatc gagaagtagc tactgggagt ctatttatca aaaaaaggaa tgcgacatag
     6121 gtcacacccc ttttttattt tctttgaaac atctttcggt actcgacagc atacaagaca
     6181 aaagattgta aaagaccgta agtgatgacg gcaaaaatga ctctcaccca cagcgttggt
     6241 aattctggga aaagcagcaa aaggatcatg ccaacataga tgacagcagt cgctactttg
     6301 ccataccact tcgctccact taatttgata tttttagata gcccgattaa tccagctaca
     6361 aacataaagc cttcttttat caagaataga ccaaacaaca gccaaagcca cggtcgatac
     6421 catgccatag acaaaatcag aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc
     6481 ttccctaagt ctgttacctg attatatctc cgagcaatga ccccgtctag ataatcagac
     6541 aaacctgaga caacaatgac cgctcctgct atataaaatg actgtctcag cacaaaataa
     6601 agataaatcg gcagcaaaaa aatacggaat atcgataaaa gattcggaat cgtttgtatt
     6661 tctttacgcc agtctgtgct taatttcatt ttatcgccct acctcctcag cactcttttc
     6721 tttttatttt agcttacacg gggaataaaa ccaatgattt cactgttttt atttttcttt
     6781 acagccactg atattatgag ctaatttgag ataatcactt gctgttttca tcgtttactc
     6841 ttctttcttc ctgtcgtttg cgcatttctt ttactagtac tttatgagct ttttttgatc
     6901 ctcgctctag gtcacgctgc cgtttacgtg ttttataatc gataaacaga ctgtctaaat
     6961 cgtaacctgt cgggtacaga tcttctgctg aacgcaccag acgaacacgc tgacgaggca
     7021 cgtggattat ttttttgtct aaatatacat caatcgtatc ttcacctata tcttcataaa
     7081 ctaacgcttc tttttggtat tcattgacaa atatccgatc accttttgca aagagctggc
     7141 gttctttttt attctccttc ctatttgtaa ctgctgtctt aggaagaaat tcttttttct
     7201 gtgtccgata ggctttttcc gccaagtaac gctctgcttg atggatcaat ttcatcgaca
     7261 tatccatttt atgagcaatc cacaaagctt ggctttctcc tgtttctcca acccgcaact
     7321 gatatttagg acgaagtgct tcccgatcga aagccatagc agccggcaca aaatcttcat
     7381 gatctccggc gaatttcttg atttctccat aatgggtcgt ggcaatgatc aatgcccctt
     7441 gctcatacat agattccatg attgcgatcg ctagtgcagc cccttcattt ggctctgttc
     7501 cactgccaat ctcatctaaa agtaccaacg tattcctttt tatctttttc aaaatagcag
     7561 cgatgttttg catatgacca gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac
     7621 cgacatcgac gaaaatctca tcaaagacag caagctctgt cccttctttt gccggaactt
     7681 gcataccgaa catcgccatc aaagtcaata aaccgactgt cttcaacacg actgttttgc
     7741 ctccggcatt tgctccggtg ataatcaagc ctcgatagtc tttaccgatt tcaacatcca
     7801 atggtaccgc atgctcaggt aaaaatggat gtctgccttg cttgataatg atatgttccg
     7861 atttattgat tctaggtgtg cttccattga tttctcggct aaattttgcc cgagcaaaga
     7921 tgatatctaa caccgtgatc gtttcgatca ataaatcgat cacttcttcg ttttctgcga
     7981 tagcaccagt caatgccgcc aggacttgat attcttcact gatttcttca gcttttaaca
     8041 actgatagtg ttcattcaat ttagaaatca cagttggttc aataaaaacc gtcgttcttt
     8101 tatttgattc atcgataatc gttccatcta ttttattttt atagctggct ttgataggaa
     8161 tcgtatagta ttcgcctttt tgaacaatca ttgcttcttg gatcatttct ttgtttttcg
     8221 gatgacgcaa gaatttcagt aattttgact ggatttcttt ttcgattagt tgtaattgtt
     8281 tgcgtacttt acgcaaatta cgtgacgcgt cgtcactgac tttattattt tttattttct
     8341 ggtcaatcaa ctcttctaca tttatcaaat caggaagatg tttgctataa gcaaacaaca
     8401 agggtgtctg gtattgattc ttatcaaaaa actttgtgat catccgactg cttctaagaa
     8461 aatccgcata ttcgatcaaa tcggttggct gcagaatcaa cccttttttg acttgttctg
     8521 tcagtgcatc gatcctcggc aaccccataa atggaacatg ctgactgctt tctaagatca
     8581 agcgtgcttc tcttgtttct tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg
     8641 tcatatcgct gattcgtttt ttgctatagt ttccaattgc tcttgcttgg atttcctttt
     8701 tgatccgatc aaattgtaat tgactaatca tttcgttgtc catattctct ccttttcttg
     8761 aagagatacc tgtcccaaaa taaataaggg cctagagata aataaaagga tagaacacag
     8821 cttaaaaaag tcatgtccta tccttattag aatacaaaga tattgttttc ctgacaggta
     8881 ccgtttcata agcacaccaa ataaactttg tttattggct attcgactta ctgattagat
     8941 accagctaca ctcacaaaac ataccccatt cttctaggtc ttatttattt tccacttaac
     9001 tatagcaaga cacattttgg ttgtcaaact ttagtatgtt ttatagattt acaccattaa
     9061 tgaatagaca cctaccgcga taattgctcc gatcattgga cctgcgattg gtacccatga
     9121 atatgcccaa tctgaagttc ctttattagc tattggcaat accgcatgtg ctaatcgagg
     9181 accaaagtcc cgagcagggt tgattgcata tcctgttggc cctcctaatg aaagaccgat
     9241 ggctaagatc aatccaccta cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc
     9301 aaaagctagc aacccaagaa ctaatacaaa tgtaccaatt aattctgtga tcacatttgc
     9361 tggataattc cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt
     9421 cgttgcattc caatgtggca aataagaaag ccaaacaaga atggcccctg caaaagctcc
     9481 taaaacttgc gcaatgatga aaggaacgac aagattccaa ctaattgccc ctgtcatcgc
     9541 catagctaga gagactgcag gattcaaatg agctgggccc atgaatccag aaatatagac
     9601 agccattgtt acagcgagtc cccaaccaaa tgcgatgaca atccagccag aagcctgtgc
     9661 tttacttttg tttaaattca cagccgcaca cacaccatct ccaagcaaaa ctaggatcag
     9721 tgtcccaata aactctccca tgatttgagt catatcactg ttcatttatt tctccccctt
     9781 tagttcccgc agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt
     9841 ctcctcttct gtccattcaa aatatccagc aattgcatcg atcacaggtt gcttgatctc
     9901 atcaagctga tctcgttcaa acaacagatg atttgtacga cgaatgagat aatcgcctgg
     9961 ggccaaaacc atttcttctt ctaaaccata acgtaaccga gctgactcag cgagactcaa
    10021 accaggataa ggtgccattt cttttgctaa ttcaaagata cgtcgggcat tagtcccgta
    10081 aaaatcagcg atataagtag catcttcttc ctctaaaccg gctgctactc cttcttttgc
    10141 taattctgtc actgtttctt ctaatttagt tggatcgaag tttccacctg aaatctgata
    10201 ttttttagag tcgatttctt tcgtctctat tccgtattct tcttttaata attggcgtat
    10261 caatcgcaaa gctccttctg ccatcttacg gtaatcggtg attttcccac cagataaagt
    10321 cagcaaacca tctggttctc tttctaaaga actacctctg gaaatcgtag aaggtgcttt
    10381 ttcatcacgg ctgttttcca aatggttcaa cacgtcttct acttcagcac gagaaacttt
    10441 gttttcctta tattcactta ctgtatcgac aactttattg aagctcttgt ctgaaatcga
    10501 tccattatct ccaccattgt aatcagaacc agaattacca atcaacagtg gacgaagtcc
    10561 tgcccagctt gcttcgatat cattcaatgt gatatttgct tctggatagc gatgattgat
    10621 cacatccaat agataatcta catcttcttg tgtgactttc ggatccgtaa aatctccttg
    10681 gtaatccgta tctgtcgtac caaagtaagt cttgttttct cttggaatag caaaaaccat
    10741 ccgcccatct tgctttcctg tatcgaagta tgtgggttgc ggtacaggca gttttttcgc
    10801 atctacaact aaatggatcc ctttagttgg acgcatttta ggagagactg gacgcgtaaa
    10861 attcaagttt cgtactttat ctacccaagg accactcgta ttgatcacta attttgcttt
    10921 gatttcgatc acttcatctg tcagcaggtc acgagctttg acgccaacga tttgatcgcc
    10981 atcatataaa aagccaaccg ctttcatttt acttactaga taagccccat cttcggcagc
    11041 ctttttgata ttatcaatca ctaaacgggc atcattgttg cggaaatcta gatagacacc
    11101 cgcgcctttt agcccttctt ttttcaaaaa cggttctctt tccaatactt cttctggtgt
    11161 cagcgtataa ttctcatatt tggttcctgt cacatttgcc agtttgtcat aaaggtccat
    11221 tgctactttg accgagaaca tattgaaggt tgttgctcct tcgccctcat agattggcaa
    11281 aagcattgga tctggttttg ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc
    11341 aactgtgtca gctactactt ccacatcaaa tgtcttcaga taacgaatac cgccatgaac
    11401 aagtttggtc gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt
    11461 tttgattcct gatgctgctg actggactgc cacaccggca ccagtgattc cgccaccgat
    11521 gatcaacaga tccaattctt cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt
    11581 tgaaaacata tggatttcct ccttattcaa atgcttgggt agcttttact gcttttttcc
    11641 aaccgttgta aagtttttct cggcgctctt cctccatttg aacttcaaat agcttgccag
    11701 cttcataaaa ttcgcggatc tcgtctgtat ctttccaata accgacagct aatcctgcta
    11761 aaaatgctgc ccctagcgcc gttgtttcca agttttctgc tcgttggatc ggtacattca
    11821 agatatctgt ttggaattgc atcagatatt cattgtttgc tgcaccgcca tctactttca
    11881 atacaggaat cttgattcca gtgtcatctt gcatcgtatc gatgatatct ctcacttgat
    11941 aagcaatcga ctgtaaagta gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc
    12001 caaacatagc ccctcgtgct gcctgatccc aataaggagc acctagccca acgaatgcag
    12061 gaacaacata aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag
    12121 ctttttgcaa catttgaagt ccatctctca gccattgaat cgaagaacca gccacaaaaa
    12181 tacttccttc taaagcatag taaacctttc catttatgcc gtaaccaatt gtcgtcaata
    12241 aattattttt ggataattgc ggtttttcac cggtattcat gacaataaat gaacctgtac
    12301 cataagtatt cttgaccatg cctggttcga atgccatttg tccgaataat gcggcttgct
    12361 ggtctcctgc catccctgaa attggtgctt cgcttccata aaaatgataa ccttgcgtat
    12421 aaccataaac ttctgagtta gaagtgactt ttggtaacat cactcgagga atatttagta
    12481 aatctaaaat ctcctgatcc caatctagct gatggatatt gaacaacatc gtccgactgg
    12541 cattcgagta atcagtaaca tgtgcttgtc catcggtcag tttccatact aaccaagaat
    12601 cgatcgttcc aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct tctacatgat
    12661 caagaatcca gcgtatcttc gttgctgaga aataggcatc gatcactaac cctgtttttt
    12721 cgtggaaaaa ttcttgatag ccttcatttt ttaagttatc tgcaattcca gctgactgtc
    12781 tggattgcca aacaactgca tgatagattg gacgtcctgt atctttttcc catattactg
    12841 ttgtttcacg ctggttcgtt atgccaatcc cagcaatctg atttggtttg atccctgatt
    12901 cgatgaacgc tccggcaata actgactgta cagaattcca gatttcattt gcatcatgtt
    12961 ccacccaact ttcttgcggg aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc
    13021 ttcctttctt gtcaaagata attgctcgtg agctagttgt accttggtca atcgccatga
    13081 tatacgttga ttctgtcatt gtgattccct ccattatatg caaacgcttt ctttaactac
    13141 tcttatcata ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt tttttctttt
    13201 tttgacagtt ttttattatg aagctatttt ttaatcaatt cttttctttt ttcagcgtct
    13261 ctattcttct tttcaatcga ataatgtcgt aaggtgacgc aaattctgac agataatatt
    13321 cacactgttt tttgtttctc tggcaaaatg tgatcaccaa atcatattct ttaccttctt
    13381 cataacttcc aatctcaata ccgccgattc ccctcagttc tctgatcaga tattgttgaa
    13441 aagcaatacg ataaatcggc agactttcta aatcgattcc aatcactact ggttttgcaa
    13501 tataaaaatc gatcatcaaa agaacattgg catactggat catcaggtaa ctaagctcac
    13561 tatcttcagc tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta
    13621 actgtgttaa ggaatgcccg agcattttct gctgctgttc caaaagccga tcacgctcat
    13681 agacttcgat ctttccaaca aagaaaaacc attcattgtt gatctgggaa atttggtagc
    13741 ctactgcctt ttcttctttg attttcaaac gacgaggacg ataatgccat aacatcgttt
    13801 cccgaatgta agtatctaaa acagcggttg gtaacttttt gctccgtgtc aaatcatact
    13861 gataaaatat ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa aacatcattg
    13921 tttcgtatac attcagttct gctgctgttc gactaagatg catcacgatg attggatcga
    13981 ttgcttgata aagccggtcc gactgataga gtaatttctt ttctttcaaa gttgcgtacg
    14041 tacttttttc attcaatagt ctttttttcg taatcccgag ccagcaagca atctgtgctt
    14101 ctgctgatgc cgtaaacgtt gtttctaata cacggtccaa tcctagaatg aacggtcttt
    14161 tttctggtgt attttgcaaa aaaagcggtc gctgatcttc cgggatatat tggaatagtt
    14221 cataataaaa atatcgtatc tgtagctctt ctccgtataa ttgaccgttt ttgatttgta
    14281 atccaaattc ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca gaagactcac
    14341 tgatattaaa cgctgtcgct aatcgaacga tggatacttg ctgatgttct aataaataga
    14401 cgagcatctt aaacttcaag ctttcctgca ataaaaacaa tagaattttt tccaaactta
    14461 catcttcgtc caacacaagc agcgcctgat actcatttcg ctgtacttct actttttttt
    14521 ccatcattcg gccaatcaat tcaatatctt ccaaatattg atcaaacgag gcttttgaca
    14581 gttggacatg ctctctcaaa ccattgatag aggctgttcc cccttgcaga acaaggtttc
    14641 gcaaaatcag gatctgtaac tgctcttttc tttctaataa tgcttcaatc ttcataggca
    14701 gtattcgcca cctttgacca caccatatct ggcacacggt attctggctg gaaaaatagc
    14761 tcatgttctt ctaactgttc aaacgtccat ccttcttcta ctgcaattgt cagttggtca
    14821 agaaggtgga acatctcttt tttgctttgg agctgtgctc caagaatctt tttttcagaa
    14881 tcaaatagaa atttgagttt aactttgctt tgatttattg ctgtaagctt gtagtctacg
    14941 tatcctgttt caattttttg gggataaaaa atgctctctt cttctgtcaa acctacgctt
    15001 cctaagaaat agccaaacca ctgattgccg actggacgat aggttccctg atcctttgtc
    15061 tgtgagccac taattgtctt gctgacattt cgtgctgtac ggacagcgtt atttactaga
    15121 gaaacataca ttttttcatt tgttggacga aaagttactt ggatcgcatc gccaatcgca
    15181 taaacgtctt tttgagacgt ttgcaagtag tcatctaccc agatcgttcc atcatcattc
    15241 aatgttaatt gttcttccca catctgattg tcggggcgag aatgattagc cagcaaaaca
    15301 agatcatagt tctcctttct tttttccgtc tctacaatag cggtttcatt ttcggtcaaa
    15361 gacacaactt gttcttctaa aaaaagtgaa agcccttgat ttttcatttc ctttactaac
    15421 ggttctgtca tttcaggatc tagataacga aaaagaattg actttcggct ttcgtaaaga
    15481 tggatttgtt tttttcttga tgccaaccct tcagctattt cgattcctgc ttgtcctgca
    15541 ccaataattg caattttctt tgcattcatg atcttttgct gaagagccgt tacgttttgt
    15601 atctttttaa ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa cttttgattt
    15661 gaaccagtcg ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc taaaacgact
    15721 tttttttctt tcaatcctat tactgtcttt tctgtgtaaa catggattcg atagcggtca
    15781 gtcagttctt ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt tccttttaat
    15841 atcaaattga acccgctggg aatccatcct aacttcgatt gcttttcaat caaatatatt
    15901 tcacttgttg gcatcaattg gcgaagataa atagccgcat gaatccctgc aaacgacccg
    15961 ccgacaatta caatgttcat ctgcattcct ctttcttata aggtgttcta ctgctaactt
    16021 ctcaatcaga aaacacagta ttcataactc gctttcaacc ttatgataca tagcgatgca
    16081 gcagaatcaa ccctctccat cataccgtgt tctaggtctg cttctcaata atgtactagt
    16141 ggacctaatt gaagagtatc atttctgaag agatgtttca aaaaatgtac tcttataaaa
    16201 gaaaaaaact cgccttttac gtacctctgt ccaaatagga aacagaaaag aacgaaaaga
    16261 cgagttttag cgtgtgtaat gtaatagtta gaatagacac atcatttatt tttaattctt
    16321 taatgcttca acaactgctt cgcctaattg atgagcagat tgaggatttt gccctgtaag
    16381 aagccgccca tcaatttgaa ccacttctga aaaagctgct ccagactgat agtttgctcc
    16441 tttttctttc aaagcatctt ctgctaagaa tggtacttca ttcgttgtac catttgcttc
    16501 ctcttcttca ttactgaacc cagtcactgt acgtccagct aaaatagatt ttccagactc
    16561 atctgtaact ggcaacaatc ctacaacacc gtgacaaaca gctgagacca gcccttcatt
    16621 tcgatagatt tgtgaagcga ctttcccaag atctattgct tctggaaaat cccacattgt
    16681 tccgtggcca ccagcataat aaaccgctgc ataatcattt gcatccacat catcaggacg
    16741 taaggtattt cccaaggccc gatttctgaa atcttcatct tcataatagg cccaatcgat
    16801 ttcatccata ttcaaaacac tttgcggttc taaaggtaca tatccgcctt taggactgac
    16861 aaaatctgca tcgatcccat tttttactaa tacatcataa aaatgagtca gttcacttaa
    16921 ccacaaacct gtaggacgct cgatcttttc gtacttttct acatttgtta atacgatcaa
    16981 tactttcatt ttcccatcct ctttctataa aaattgcgtt attgataacg tgctttcaag
    17041 aaattatata atgggcttgg caaataaagc tctttatatg cttcttcaaa aggtttcgtt
    17101 acaaattcat cctcacggtt ttccaataat ttctctgccc agtcaggatc gacgatcatt
    17161 tgctgtccaa ttgctgcaag ttctgcatat ttcaatacct gctctacatc ttcgcgattg
    17221 cggatcccac cgacaccaac taaagggatc tttccttgaa tcgtgttatg gaccacttca
    17281 agaatcgatt tatcgttgta tttatcagaa cgagctattc gatcatacat atttaatgaa
    17341 acatgcagat aatctagttt tgactcacgt aatcgttcaa gaagccagat cgtgtcttca
    17401 aaacggatac ctggtgtttc atattcttct ggtgagaaac gataccctac gatgaatggt
    17461 tttttagcat attgttcaac cgctgagaaa accgttgtta ataattcttc aataaaacga
    17521 taacgatttt ccaagctacc gccccattga tcctctcgtc tattcgaatg cggagagaaa
    17581 aattgttgga tcaaatacgt atttgctcca tgcaactcga ttccatcaaa tcctgattca
    17641 atcgctctac gtgtggcttt gccaaatgct tggattgttt caacgatttc ttcttcactc
    17701 attgctcgtg gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc agatacaggc
    17761 tgttctccag ataaagttgc tcgacttgtc attcgtccac cgtggaaaat ctgaataatc
    17821 gcttttgcgc cttctccttg gattgctttc gctagctcac ttaatcgagg aatcatttca
    17881 tcatgagcga tgctgagttc acctggccag ccttttccga tatcttggac atttgcagca
    17941 ccagtaataa cagcaccaag acctttagaa cgttgtttat aataagtgat ctcatcttcg
    18001 gtgatcactc cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat caatcggttc
    18061 ttcaattgaa ctcctgaagg taaagtaatt gattgatcaa attgtgacat tgtcattcct
    18121 cccataattc gctctgatta acctatcgag aatcatctat cattaaacca ctcgattagt
    18181 tgtacatgca actaatttgc ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc
    18241 attcattgtc tgggctaatt ctttacttgc ttcatttccc aaaactgctt tgtatgactg
    18301 atgaagttct tcccagctac catcgacttc cttagcaaat tggcgtcctt gttctgtaag
    18361 ataaactaat gacaagcgac cttcgctaac tgttttgacg tatcctttat attctaattt
    18421 ttcaacaaaa cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa tatctttttg
    18481 tggtattcca tctttttctt ttaatatcaa taaaataaaa ccataagttg gtgccagacc
    18541 tgtccttcta aacgcttcat cagctaattt attcaacatg cgatctagct ttttcacgct
    18601 aaagaaaaga cattcatcta acgctttttc aattggcttt tccatttcat cccctccatt
    18661 ggcgtacata tcatagtatg atttttattt tttgtcaaaa aacacggaat aatgtccgcc
    18721 tcctctgtct agatttgaaa aaaagaagta gccatcttca aagaatttcg gcttgttttc
    18781 gacaaaactc cttctggaac accgtgaatc agaggttgtg aaaaagtcgt ttagttttga
    18841 gtatgaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata
    18901 ccgaaaaact ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat
    18961 caagtaataa gaacagccaa acaaaaatag ttcctcatat tttgtcgttt tttgagctaa
    19021 tgccgatggt cagctgattg tacaccgttt attgagaggt cgtgagcctg ccgttgaact
    19081 ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg ttttatgact atggtgactt
    19141 gtgatcgagg agttggcagg agaatactgt ttattcgtaa agaaggggct gtgacaagac
    19201 ttttgtcaca gtcccttatt tccttttatt ataggatttc gccatttgat tggatgactt
    19261 gtctgtacca atcaaatgat tttttcttta accgtttctt tgtgccattc ccttcgtcat
    19321 cttgatcaac atagatatat ccataacgtt ttgacatttg tgaagtagaa gcgctgacga
    19381 tatcaatgca gccccacgac gtataaccaa ataaatctac tccatctttg atagcttcct
    19441 tcatctgctc aatatgctgt ttcaaatagt caatgcggta atcgtcttga acggttccat
    19501 cttcttctac tttgtctata gctccgagtc cgttttcagc tatgaacagt ggtttttgat
    19561 acctatcata taattgattc aaagcaattc tcaaaccctt aggatcaatc tgccagcccc
    19621 attcacttga tggaagatac ggatttttaa ttcctccagt tattaagttt ccttggaccc
    19681 cttcccaatt ttctggatga gcactgactg cactagacat gtagtagcta aaagaaacaa
    19741 aatcaacagg gtaggctgct aagatttctt catcacctac ttctattttt aagttgacac
    19801 ctttttcttc aaacattcgt tgcgtatacg ctggataata tcctcttgct tgaacatcac
    19861 taaagaacaa agaagttctt tgctgctcaa ttgttttttg aatatcatcc ggatcacaag
    19921 aatacggaac aagaagctga tagctgatca tacaaccgac ttgtgaacct ttgataattt
    19981 catgacaagc ttttgtagct aaagcacttg ctataaactg atggtgtgca ccttggtatt
    20041 tcgcttgttc caaatgggga tggttttctt caattactcc tacgctaaca aaagggtgat
    20101 gttttacaca gttgatctca ttgaaagtca tccaatattt tactttattt ttatagcgtt
    20161 tgaatactgt ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta ggccatccac
    20221 catattctgt aaccaaatat aacggcattt cataatggga aagagaaacg actggctcaa
    20281 tcccatattt tagacattcg tcaaacactt gatcataaaa aaataatccc gcttcattgg
    20341 gtgtttcttc atcaccatga ggaaaaattc ttgtccatgc gatagacatc cgatagcatt
    20401 taaaacccat ttcagcaaat aaacggatgt cttctttaaa gtgatgatag aaatcatttc
    20461 cgtgtctttt aggataataa tacttgctgt tgggatcctg cgccgctttt acttgctgat
    20521 gcgtcatcat atgccattga tctgtccatt tttctttagg taaatcgctg tcgaatgtat
    20581 acacatcaga cacagataat cctttttcat ctacatccca tgcaccttca cattgattgg
    20641 ccgctgtcgc tccgccccat aaaaatcctt ctggaaattt tttcatatcg atccccctaa
    20701 acttgctagt tttcttgttt gattattttt cttatgacta gtaatgatat ttttgaatct
    20761 actactattt gtaacaataa ccattgtggt cggatcatat ccctctttga ttaattcttg
    20821 caaatctact tccatcaata aatcgccttt acgtactttt tttccatcag atactgcact
    20881 ttgaaagtat ttgccattta aattcactgt atcaataccg atatggatca gtatctctgc
    20941 tccttcttca ctgactaccc caattgcatg tttcgttgga aaaactgttg aaacttcccc
    21001 gtttacagga ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga
    21061 tccactagca aatgttggat cgtttacttc agataaatca attatttcac ctgctgcagg
    21121 agtacttaac actatatcct ttgaggattc ttcttcgatt tcatccttgt agccgaaaag
    21181 aaaagttagc atacaggaac tgatcatcgc taagaaataa ctagcaacta gccaaacaaa
    21241 tccttcacca atataaatcg ggaaagacaa aatacttctt gtagcaactg ccaaagcacc
    21301 ggctcctgcg gcaccagcta ctccaccaaa tatagctcca catatacaag caatgtaaaa
    21361 tggtcgttta tatttaagag tcacaccata aacagcaggt tcagtgattc caaataatgc
    21421 tgtgataaat gcagataaag ataaactttt tactttctta tttttcgttt tcaaaaatac
    21481 cccaagtact gctcctgctt gcgcagctac ggcagtcata catgctggac ctagcgtatc
    21541 acgaccgtag aaagaaagat tgttggtcat tactggtaca agtccccaat gtaacccaaa
    21601 aatcactaac acttgccaaa atccgccgat aacagcacca gataaaacag ggctaaagtt
    21661 ataaacagct aaatatccag ctgcaatcag ctgacttgca taatctgcaa ttggacctac
    21721 aactaataaa gtaattggga ccatcacgat gatacaaatg aatggtgtca ataaatttcg
    21781 aatactttcg tgcaagcttt tgcgaatctt aggctcaata taagaaagaa cccatacagc
    21841 taaaattgct ggaataaccg tacttgtata ccttgctaga atcacgggca tccctagaaa
    21901 tcgcaaggtt atgctatcac tatatgcact gacaatcgta ggataaacga gagctgctgc
    21961 aataaccata gcgatgaaac gatctgtatt gaactttttt gcagcagtat atgccaaaaa
    22021 tataggcaag aaatagaaaa atgcatcagc tgctgcattc aaaatgatat aagttcctga
    22081 ttcagtagtt aataaattca aactcgtaca taaggcgagt actcctttga tcaaaccact
    22141 tcctgccaag acgcctaaag tcggggcaaa aacagcggcg atcatgtcta taaaagagtt
    22201 taaaatgcct tttttctcag ttcctctctt tccactagat gcatcatctt ctagtttcat
    22261 ttgtttggat aatacgtcat acacttcatt tactgcattt cctactacga cttgaaacat
    22321 tcctccagct tcgactacag tgatcacacc ttctagttgc tctaccttct ccttatctgc
    22381 cttcttatta tcttttaggg taaagcgtaa ccttgttgca caatggtaaa cattgctgac
    22441 attttcttcg cctccgacaa atcggacaat atctttcgct aattgtgaat agtccatttt
    22501 tacttccccc ttgttaatta atgccgtagc aatccttctt acaagataag tatagttctt
    22561 agaaaacggt ttctccattc aattagtacc ccttataata gcgaacgaaa ctgtacaata
    22621 gtgtgtatgg tttacttagc cgagcaaatc actcactgct tgaaagaaaa caagtatatc
    22681 gtttctctta tagtcaaagg agacaaaaaa aacacgcttg atcttccttt gctgatgtat
    22741 aagtggtatc tcaagctgat actctgtgat ttttgtttct acttggcttt tgataagaca
    22801 gcacaacaaa gtctcggcat ttaacggaag cataactagg tcagaactag gagagattag
    22861 ttgctgaatg gaatctatct ttttatggat ttttatttga ttttcttctt tgtagtaatg
    22921 atcaagcagt tttgttttct ttttttcttc aactatggtc agcttttcac gcaaaatctg
    22981 ataaatctcc caagattcaa actcatctag aacactaggt agataaatca aatcatccca
    23041 ctcaaaaata agctcttgct cagtagtcaa aattgcatca tattccgtta ttttgtttga
    23101 ttgatttttg aaaagataca ctggttctat ccggcattct ttgatccaca tgttcatact
    23161 ttgttgaatc tgttttttta gtgtattcaa aatactaatt ggctggttac taacaatcag
    23221 tatctcccct tgttttcgaa aaggattcaa cgcttcggca agatacaaaa caaaataagc
    23281 gctttcagct aaattgggac gtaattgaaa atgtattggg aagaaacgcc gaattaaata
    23341 aatttctaaa ggataacggg agatgatctt ttgtacaaaa tgattcatgg tattatgtcc
    23401 ggcatccatc ctcgttttca tttgtttgat atgggttagc aaaaattctg tttcatggaa
    23461 caataacgaa acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt
    23521 cttctctatt tctttgtttt tttcatcatt aatcatcaaa taatttaact ctaatagtcg
    23581 attttcaatt gcttgttttt cttgattgga aaaagaatag ggtagttctg atgataactt
    23641 ttttgtgagg gtctctacca tcggaataaa taatgtatac ctgtttgttt tttctaaaag
    23701 ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga agtcctctcc
    23761 tgaaacaata taattatatg atataagaac ctcttttaat gtcttttgga cagtcggcat
    23821 gatattggaa aaaattgtac tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc
    23881 tgttccaaca agagataaga aaattctttt tgaattttct tcagcaatga tttttagtcc
    23941 ttttgtcgac gaaacttcca gttctaattt aggattgcgc tcgatccaac gcaaaatcgt
    24001 ttttatttgt aatgatacgg ctgtttttga aagaaagaaa atgtctgcta tcttttgcat
    24061 actgacatac cctttttgaa acaataagta cgtcacaata gttgaagctc ggaaatccat
    24121 gctggaatag attttatgtt tcgtcacctc ttgtatgatt tgctgtttag tttcagattc
    24181 aattgattct aaaaaataac ctgaacgttt atttgactgg ataatgattt tcttctcgta
    24241 gatagcaagc atatttttga tttcagtttg gatcgttttc ttgttggtac ctaaagcttc
    24301 agctaattct tcgcttgtta tccaatgatt cttatttctt aatactacaa gaatttggag
    24361 ttgtcgttta tttaagtcca tttgcttaca cctcctcttt gttataaaca tacaggaaaa
    24421 acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag aaagatctat
    24481 attcaaggtt tttaccatta gactggataa tttgtttata atattcaaaa ctatcttttt
    24541 tcaatctcct aaagcttccg ctcccatcat tttgtctatc cacataaata aagccatatc
    24601 gcttcctcat ttcccctgtt cctgccgata cgatatcgat tggtccccac cataagtaac
    24661 caatgatttg gcaaccatct tttattgcct cagcaatttc ctgaagatgt atttttagat
    24721 actcacaacg ttttggatcg tggattcgat tcaattcatc tggtgcttca tctagtccaa
    24781 tgccattttc aactatgaat aatggtaatt cataacggtc tgtcaattca ttcatgacat
    24841 aacgaaaccc tagaggatca attggccaac tccaaggttc gggagatttt tccttcaaaa
    24901 agggattatc gaaaccattt gctccgccag tatctacgcg catttctgct tcttttttat
    24961 agacagcgct acgataataa ctaaatgcaa tataatctgc cgtgttagct tgcaagcact
    25021 gttcgtctcc atctaacatt actggtgcac agtcttcttc cttccatgtc ctgtaaatat
    25081 aagctggata atgccctcgg cacatgacat cggagaaaaa ccatgaattg cgtttaaatt
    25141 gcagtgaacc aaatacattc tctggatcac agtcatatgc ataagtcgct aatgcagaaa
    25201 agctacacat aatacccatc ttagcatttg gatcgatttt ctttaataat tgagtagcac
    25261 gggcgttcgc tagaaacata tgatgacagg cttgataaat cacttttttt gttagtgtag
    25321 caatgggttc aagtttatct ctagcaggga atgtaaccaa tccagcagct gcataaggca
    25381 tacgaaaagc attattgatc tcattaaagg tcatccaata tttcactttc ccttggtagc
    25441 gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat taattttcga ttttgccacc
    25501 ccccatactc tcctaccaat gctaaggggg tttcataatg actaagagta attactggct
    25561 cgatatcata tcgatttaat gtattgatta gtctgtcata aaatacgaga ccagcctcat
    25621 ttggcttctg ttcgtctcct cttggaaata ttcgtgccca tgagatgctt gttcgaaaag
    25681 cttttaaacc taactccttc agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa
    25741 tcgcttcatg agaaagataa tggagtgatt cgtctaaagc aatctcccaa atcttttttt
    25801 cttcattcca ctgaatacta ggatgctggt tcatgatccc gataagcgta tctgtcacat
    25861 taggtaattt gccgtcttcc aaccatgcac cttcacattg attagcagct actgctccgc
    25921 cccataaaaa attttctgga aatatttcct gatccataac tatctctcct cgctatcaac
    25981 aatttcttac ttgaagtata gctcgattca tcaaaacaag atattctaac tatttcggca
    26041 gaagagaaga aaaaaattga atgctactct tcatttttaa tatttatata taacaaatac
    26101 ataagacgat ccccgaaaga aaacactcgt taaccatcta agcaaacgat ttattttact
    26161 tccaacacaa atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga
    26221 ggataatttg acacaataac ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca
    26281 ctggacatat tacaaataac gagccattgc tggtcttcat atatgcgaga ataggcaaag
    26341 acatgctcag gcgtcttttc cactaattca tagtctcccc agacgatgat gtcatgtttt
    26401 ctacgcaaat cgatcaattt cttgtacata taaaaaacag aatcttgatc tgctaacgct
    26461 tcttctgcat tgatttcttt ataatttgga ttgactcgaa gccatggaat tccagtagta
    26521 aacccagcat gttcacgggc gttccattgc ataggcgtcc tagcattgtc tcttcctttc
    26581 gcattgattg attccaagat ttcctctttt gtaaatccag aagaaatcct ttcgttatac
    26641 atattgattg tctcaatatc ttttgcttcc gcgatatcat cgattgggca attcgtcatg
    26701 cctaactctt ctccttgata gatataaggg gtgcctttca tcatatggag caagatagca
    26761 aacatcttgg cactttcttt tcgatacaca tcgtcatttc cccaacgaga aacaattcgc
    26821 ggaagatcgt gattattcca aaataaacta ttccatcctt catcacctaa agatgtttgc
    26881 catttggata atactttttt cagttcagag atactcaagg gtttcaaatc ccatttgtct
    26941 ttgccttctt gctgatctaa tcctatgtgt tcgaattgga agaccatgga taattcattt
    27001 cttttaggat tggaataaag tttagcgatt tcggtagttg ctccccatgt ttcgccaaca
    27061 gtcatcagat ttttgtcacc aaaagttgct tgattcattt cctgaagata ctcatgcagt
    27121 ttaggtccat tacttgtgat ttctttatcc ggttctttgc caatcatatc gatcacgtcc
    27181 atacgaaagc ccccaatacc tttatcgatc cagaaattca tcatttgata aatctcttgg
    27241 cgaacctgtt tatttttcca attcaaatca ggttgacgct tgctatacaa gtgcagaaaa
    27301 tattgccccg ttttttcatc caactgccaa gcagaaccac tgaatattga gcgcaatcta
    27361 ttaggctctt ttccattgac cggatcacgc caaatataat agtcccgata aggattttct
    27421 ttatttttac gggcctcaat gaaccacgga tgttcatcag aagtatgatt caccaccaga
    27481 tccatgatga tacggatatt tcttttcttt gcttcttcaa tcagttcttc catttcttcc
    27541 atcgtaccga attctgccat gattgcttga taattactga tatcataccc attgtcatca
    27601 ttcggagact cgtatactgg agaaagccaa attgcatcaa tacctaaata cgccagataa
    27661 tccaacctgg aaataatccc ccgcaaatct ccaataccat cttgatttga atcttgaaaa
    27721 cttcttggat agatttgata aacaactgaa tggtgccacc atttttgttc catgattttc
    27781 ctcctataaa tggatgtttc tattttttgc ttaatcattt attttagtct ctctcactaa
    27841 aaattgtaac gctttcaaat agctattgca ccttgttatc ggtaacagaa taaaacaaaa
    27901 aaacagctga ggaaattccc tcaactgttt tttgaaagga ttgatttatg cagggaagaa
    27961 tgctgatttc acaacttctt tataaacggt gttcactcag ctgtcccttg gtattagctc
    28021 aaaaaacggc aaaatatgag gaactatttt ttcgtttttt tctctaatac tcaggccaga
    28081 acactgattt cacaacctct ttataaacgg tgttcactca gctgtccctc ggtattagct
    28141 caaaaaacgg caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag
    28201 aacgctgatt tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac
    28261 aaagaacaga ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt
    28321 aattggatag aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat
    28381 gccgatcaag atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg
    28441 caaagattgt gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt
    28501 aacaactgat agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat
    28561 agctgtgata cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc
    28621 aacaggagaa gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc
    28681 tttccctaca cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac
    28741 taaagttcct gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat
    28801 ttgaagagaa ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact
    28861 tggtgctgcg gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat
    28921 cgttgtagct gctatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc
    28981 agttacgact aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc
    29041 tactaatgta gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat
    29101 aaatagaccg ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat
    29161 catttcccgc agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc
    29221 ggctaatgct gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc
    29281 attgatccca agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac
    29341 acagccgatc gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga
    29401 agcacctaat acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa
    29461 tcctgccagt acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag
    29521 tattttttcc ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa
    29581 atttcgattt attaataaaa caacgtg
//
LOCUS       DACDID010000016        29568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDID010000016 DACDID010000000
VERSION     DACDID010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.016"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            403..1512
                     /gene="pdhA"
                     /locus_tag="IV236_000582"
     CDS             403..1512
                     /gene="pdhA"
                     /locus_tag="IV236_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8770803.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1515..2492
                     /locus_tag="IV236_000583"
     CDS             1515..2492
                     /locus_tag="IV236_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8770804.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2517..4160
                     /locus_tag="IV236_000584"
     CDS             2517..4160
                     /locus_tag="IV236_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8770805.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4168..5574
                     /gene="lpdA"
                     /locus_tag="IV236_000585"
     CDS             4168..5574
                     /gene="lpdA"
                     /locus_tag="IV236_000585"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8770806.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5736..6011
                     /locus_tag="IV236_000586"
     CDS             5736..6011
                     /locus_tag="IV236_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8770807.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6017..6784
                     /locus_tag="IV236_000587"
     CDS             6017..6784
                     /locus_tag="IV236_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8770808.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6952..8781
                     /gene="typA"
                     /locus_tag="IV236_000588"
     CDS             6952..8781
                     /gene="typA"
                     /locus_tag="IV236_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8770809.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9042..10814
                     /gene="recQ"
                     /locus_tag="IV236_000589"
     CDS             9042..10814
                     /gene="recQ"
                     /locus_tag="IV236_000589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8770810.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10928..11230
                     /locus_tag="IV236_000590"
     CDS             10928..11230
                     /locus_tag="IV236_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8770811.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11233..12396
                     /locus_tag="IV236_000591"
     CDS             11233..12396
                     /locus_tag="IV236_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8770812.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12422..15850
                     /locus_tag="IV236_000592"
     CDS             12422..15850
                     /locus_tag="IV236_000592"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8770813.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15871..16995
                     /locus_tag="IV236_000593"
     CDS             15871..16995
                     /locus_tag="IV236_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770814.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16985..17422
                     /locus_tag="IV236_000594"
     CDS             16985..17422
                     /locus_tag="IV236_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8770815.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17406..17744
                     /locus_tag="IV236_000595"
     CDS             17406..17744
                     /locus_tag="IV236_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8770816.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17863..18423
                     /gene="rsmD"
                     /locus_tag="IV236_000596"
     CDS             17863..18423
                     /gene="rsmD"
                     /locus_tag="IV236_000596"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8770817.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18420..18911
                     /gene="coaD"
                     /locus_tag="IV236_000597"
     CDS             18420..18911
                     /gene="coaD"
                     /locus_tag="IV236_000597"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8770818.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18901..19944
                     /locus_tag="IV236_000598"
     CDS             18901..19944
                     /locus_tag="IV236_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8770819.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20103..20831
                     /locus_tag="IV236_000599"
     CDS             20103..20831
                     /locus_tag="IV236_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8770820.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20947..21192
                     /locus_tag="IV236_000600"
     CDS             20947..21192
                     /locus_tag="IV236_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8770821.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21189..21407
                     /locus_tag="IV236_000601"
     CDS             21189..21407
                     /locus_tag="IV236_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8770822.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21543..22082
                     /locus_tag="IV236_000602"
     CDS             21543..22082
                     /locus_tag="IV236_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8770823.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22075..24348
                     /locus_tag="IV236_000603"
     CDS             22075..24348
                     /locus_tag="IV236_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8770824.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24386..25432
                     /gene="holA"
                     /locus_tag="IV236_000604"
     CDS             24386..25432
                     /gene="holA"
                     /locus_tag="IV236_000604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8770825.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25521..25775)
                     /locus_tag="IV236_000605"
     CDS             complement(25521..25775)
                     /locus_tag="IV236_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8770826.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26175..26444)
                     /locus_tag="IV236_000606"
     CDS             complement(26175..26444)
                     /locus_tag="IV236_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770827.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26914..27369)
                     /locus_tag="IV236_000607"
     CDS             complement(26914..27369)
                     /locus_tag="IV236_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8770828.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27507..29435)
                     /locus_tag="IV236_000608"
     CDS             complement(27507..29435)
                     /locus_tag="IV236_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8770829.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9854 a         5160 c         6259 g         8295 t
ORIGIN      
        1 tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca ttatttgtac
       61 gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata acaccaacgt
      121 ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa
      181 aataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg cggattggca
      241 taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata aatccatgat
      301 aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt caatcgcaga
      361 atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa caaaaaagca
      421 ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt atatcaagca
      481 cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc cgatgatgaa
      541 ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg
      601 ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga ggcaagtcag
      661 ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta tcgggatgta
      721 cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc tcgaggacat
      781 gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat tatcatcggt
      841 gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa caagaaaaat
      901 gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta cgaagcaatc
      961 aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg
     1021 atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca
     1081 gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata tactgtatct
     1141 aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga aacactgact
     1201 tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg tacaaaagac
     1261 acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata tttgacggaa
     1321 aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa agaagaaatc
     1381 aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa
     1441 aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag
     1501 gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat gccttagcac
     1561 ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg aaaaacggag
     1621 gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga gtattcgata
     1681 ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc
     1741 gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac gaagtagtag
     1801 ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc atcacaattc
     1861 gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat ttagaaggtt
     1921 tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat gatgcaaaag
     1981 gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa cacatgaaac
     2041 tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct ttggataaag
     2101 cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca atggtgcgtg
     2161 aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa atcattgatc
     2221 ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa aaaactggtc
     2281 gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg
     2341 agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt tctgcacctg
     2401 atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca aaagatatcg
     2461 aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg
     2521 cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
     2581 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
     2641 acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc
     2701 cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca ccaggacacg
     2761 aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct aaaccggctg
     2821 ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc ttccaattta
     2881 agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac
     2941 caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac aaatcagtag
     3001 aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca gaaggcactg
     3061 tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt
     3121 caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa
     3181 gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct gttcgtcaat
     3241 ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa ggtggcagag
     3301 taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca ccagcgaaat
     3361 cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag
     3421 ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt gtagcaatga
     3481 cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca gctccacacg
     3541 tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa cgatttaaag
     3601 aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg aaagctttaa
     3661 cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca aaccaagaaa
     3721 tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac ggactatatg
     3781 taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg
     3841 agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc aatggaacaa
     3901 ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc atcaactatc
     3961 cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta aatgctgaag
     4021 gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag
     4081 atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct gatccagagc
     4141 tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt agaattagat
     4201 acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc tgctgaaatg
     4261 ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt gaatgtcggc
     4321 tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc actagattct
     4381 gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg
     4441 aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt aaaaaaacat
     4501 aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact acgcgtcatt
     4561 catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac aggttcccgt
     4621 ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac tggcggatta
     4681 gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa
     4741 ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg ctcaccacaa
     4801 attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt caagaaaaaa
     4861 ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg
     4921 acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc
     4981 actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg tgtcgaagta
     5041 ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc taacatctat
     5101 gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta tgaagcaaaa
     5161 atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc aatgccggca
     5221 gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga agcaaaagat
     5281 gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg ggcattatcg
     5341 cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa cgtgcttatt
     5401 ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc tttagcaatc
     5461 gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc atctcttggc
     5521 gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat ttgatttgaa
     5581 taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga attatcaata
     5641 atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac ttcctatgct
     5701 acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag tatccacttg
     5761 atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac
     5821 gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag cagtttaaaa
     5881 ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa aaagcttccg
     5941 gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa
     6001 tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc ttgaagaagc
     6061 tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac
     6121 ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa aaatccattc
     6181 ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat tatcggatct
     6241 cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg tgattgaagg
     6301 ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc ttggtttcat
     6361 ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg tgtataaaaa
     6421 tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac ttggtgtgaa
     6481 tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa gcagcatggg
     6541 gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag gaaaccacat
     6601 tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct tattggaaga
     6661 gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa aaagggaata
     6721 ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga aaaaagaagg
     6781 ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag attaattttc
     6841 tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata attagcagtc
     6901 gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac attgaattat
     6961 agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa aacaacatta
     7021 gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt gcaagaacgt
     7081 gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc caaaaacaca
     7141 gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca cgcggacttc
     7201 ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg
     7261 tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga acaacatatc
     7321 acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga gcatgtcgta
     7381 gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt agactttcct
     7441 gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc agcagatcaa
     7501 gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc tcctgtcgat
     7561 aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga ttatgtaggt
     7621 cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca agtagcatta
     7681 attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg tttctttgga
     7741 ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt ctcaggtatg
     7801 gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc gttacctatc
     7861 cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc accatttgcc
     7921 ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat ggcagaactt
     7981 caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg gacagtttct
     8041 ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga gggatacgaa
     8101 ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg
     8161 tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat cgagtccttg
     8221 agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca aatccgtttg
     8281 acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct ttcaatgaca
     8341 cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct tcctggtcaa
     8401 attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc aacgacttat
     8461 tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac agaagtatat
     8521 gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa catcacgaaa
     8581 gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt catcaaaaaa
     8641 ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga atattgcgaa
     8701 gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa
     8761 gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc ttgttataaa
     8821 aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg
     8881 gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa acttatttct
     8941 agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac ttgtccttcg
     9001 aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac
     9061 aatactttgg ttatgatgag tttagacctg gtcaaaaaga aattatccag aaagtcattg
     9121 atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc tgttaccagc
     9181 ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc tctttgatga
     9241 aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc aatagctcgt
     9301 tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag taccgtttac
     9361 tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg aagcgatgga
     9421 acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg catgatttcc
     9481 gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt cctgtaattg
     9541 ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga cttttaaaaa
     9601 ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga tttcaagtaa
     9661 ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac aaaaatcaat
     9721 ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat ttgcttaaaa
     9781 aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag cgaacagaaa
     9841 tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat gcatttggta
     9901 tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct ggctctctag
     9961 aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt gaagccatcc
    10021 tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag tcccaaagag
    10081 aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa tacgtgtata
    10141 ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc agtccgtgca
    10201 accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca gaagcacaga
    10261 tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg ctgatgaaag
    10321 tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat ttatctacct
    10381 atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa tatttgatat
    10441 cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta acagaaagag
    10501 ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag aaagtccagc
    10561 agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg cgaacggatc
    10621 tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca ttgaaagaaa
    10681 tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga gttggccaaa
    10741 gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg gactctaacg
    10801 ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg gttaaatctg
    10861 ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga atgggaggta
    10921 agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga
    10981 acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc
    11041 cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg gttttgctgt
    11101 ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc tagaacgaga
    11161 attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag aagttggcaa
    11221 ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta tagcatcttg
    11281 attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc tacgtcgtat
    11341 ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag tattttttgg
    11401 gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt tttgaaaaac
    11461 cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat tgttgtgttt
    11521 tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa
    11581 cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt atctaaaaga
    11641 caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc aatggttatc
    11701 ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt tatctttttg
    11761 attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt gattgtagga
    11821 gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac aaacggaaag
    11881 atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca aaatcctttc
    11941 tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat gttcaacgga
    12001 ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt acaagaagca
    12061 caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat ggggattttg
    12121 atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa
    12181 gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat ccaagtattt
    12241 gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt cccattttta
    12301 agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt cctcaacatc
    12361 agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta ggagtggttc
    12421 gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg
    12481 tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat attccgtaca
    12541 ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga ttgatgcgta
    12601 tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg ctattcatcc
    12661 gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat
    12721 catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa tcaaagcaaa
    12781 agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac caatcgcttc
    12841 tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga tcaaggcagc
    12901 tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg caagagaggg
    12961 ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag tgtatgttga
    13021 aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata cgcatggaaa
    13081 cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga
    13141 agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt
    13201 ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg
    13261 agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga
    13321 aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag gtttggatct
    13381 gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt cagccattca
    13441 atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag ggaaaatcga
    13501 tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg cttacgcggg
    13561 ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc atggtgctac
    13621 ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt
    13681 gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc aatcaggaaa
    13741 tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc gactacgaga
    13801 ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt ttccaggtat
    13861 cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc taatcacaag
    13921 aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg ctttggtcga
    13981 atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag acgcccatca
    14041 aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac gtttgactgg
    14101 agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat ttgatgtggc
    14161 ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt ctttaatgcc
    14221 taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc
    14281 tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg
    14341 aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag ctgtacggga
    14401 tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg atccaagtcg
    14461 ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac aactgggtgc
    14521 gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag cttatcgttt
    14581 gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc atgataccag
    14641 cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca tcgttgacgt
    14701 ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct tgtattatgc
    14761 actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga aattaaacca
    14821 ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga atgctcctga
    14881 aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac agcaacaggc
    14941 aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc atactgtcaa
    15001 cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg gagatatggc
    15061 actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag gcgaaacact
    15121 aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac ccgttggcgg
    15181 atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga atgaacgtcc
    15241 aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat
    15301 tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc aagtcttttt
    15361 ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca caccaacatt
    15421 tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct
    15481 tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg tactgttctt
    15541 caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca tcagtacggt
    15601 ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg
    15661 ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat
    15721 gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg gagaagtcgc
    15781 acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga ttgaagtgac
    15841 tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt ttggctgatt
    15901 ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc aaaacttgaa
    15961 gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa ataccaggag
    16021 cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct ggaaaaggat
    16081 tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag atattatgaa
    16141 aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt cacaactgtt
    16201 aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat gacgatgcaa
    16261 gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca tgatacagaa
    16321 gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc ttttgataac
    16381 gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc agttgtgtat
    16441 ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga aggccttcca
    16501 cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa
    16561 agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata caatcaaaca
    16621 cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag gcctgaggaa
    16681 atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca
    16741 gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca aaaattagaa
    16801 aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt tttatattgg
    16861 tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc tgtaggcatt
    16921 gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga aagtagtgat
    16981 agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc aagtcgctga
    17041 aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt caatggaaga
    17101 atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag catttgaaaa
    17161 tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa
    17221 agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata gtgaaacaca
    17281 gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt ctaatgaaat
    17341 caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat gcggaggtaa
    17401 ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg ctgatcgtat
    17461 gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct
    17521 caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa gaaacagaag
    17581 aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga ccggatatcg
    17641 aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa
    17701 ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct cttatgagca
    17761 gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc caagtatttt
    17821 tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat aatatcagga
    17881 aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc aactacagat
    17941 aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga gacagtattg
    18001 gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg ctgcagtcat
    18061 gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt
    18121 acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc aatcgaacgg
    18181 ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata tgcaaaacaa
    18241 aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa aaccgcagtt
    18301 atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa
    18361 ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga gggaaatcaa
    18421 tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac ttagatacgg
    18481 tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc aatacttcaa
    18541 agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct gtttctcatc
    18601 ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca gcaaaagaga
    18661 ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa tatgaacgag
    18721 agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc ttattagcaa
    18781 aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac tttggcggag
    18841 atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag cgtgaatcaa
    18901 atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat gttgatcatt
    18961 ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac gatcgatctg
    19021 aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt ctctcttacc
    19081 tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat gacccctttt
    19141 gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga atataatcaa
    19201 atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt
    19261 gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca
    19321 tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga cgggaaaagc
    19381 tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg acaaacaatc
    19441 gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct gatcgagtta
    19501 ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga gatcgaatca
    19561 gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg attgatgttc
    19621 actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa gcaaattgca
    19681 ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa
    19741 gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga tacgatttca
    19801 gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc aaaagctgcc
    19861 gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt
    19921 gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg acacgagttc
    19981 tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa
    20041 attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt cttcaatttt
    20101 aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga acaatgatag
    20161 aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg tctatgatag
    20221 ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt tatagagaaa
    20281 cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga gaagatccat
    20341 cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac accgatatca
    20401 aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg gtctatgatg
    20461 tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc aacttttctg
    20521 cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag ctgccagaac
    20581 atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc gagcctccca
    20641 aaataaatat caatacagcc actccatcag aacttcagca gattcctggg atcggatcag
    20701 tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag aaagtggaag
    20761 acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa aacttcgtta
    20821 caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg ctaatggata
    20881 ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt ttggatagga
    20941 aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa
    21001 aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa cagtcaagct
    21061 ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt atttattctc
    21121 attgggacga atgatctagc agagcataaa caactttcta aagaaacgta ccaaaaaatc
    21181 tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct ttctttatca
    21241 cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg atcaaagaat
    21301 atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg atttttatac
    21361 ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg atgacggact
    21421 tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt atatttaaag
    21481 aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga ggaatataaa
    21541 acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt actgataagt
    21601 cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta ctgttatctt
    21661 tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa aaaaggatca
    21721 ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt
    21781 acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca ctcctacagt
    21841 gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat tttccatgtt
    21901 tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat ctccatgact
    21961 atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct gtacaccaag
    22021 ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga aaacatgtct
    22081 agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt
    22141 atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc cggaattttt
    22201 ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt gtgtagcata
    22261 acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa aacaacatct
    22321 gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt gacaatggaa
    22381 ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa cacagaagca
    22441 gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc tgaaggcgag
    22501 ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc ttacttcagg
    22561 aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac catgcaaaat
    22621 cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt ccaatctgaa
    22681 tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa agatgcagga
    22741 ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt cagtctatcg
    22801 ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg tatcggatgg
    22861 ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt ttctattgca
    22921 ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa
    22981 ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg gcttctttta
    23041 ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt catgagtttt
    23101 ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc tcacgtcctg
    23161 tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc tattttaggt
    23221 agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta
    23281 tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgctgat agaagaggga
    23341 ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat tataggacgt
    23401 ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat ggataggttg
    23461 aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat ttttttgact
    23521 ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca aggagacagt
    23581 atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg aggtcagtta
    23641 cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc
    23701 gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt gacacacgac
    23761 gatacggatc atgtgggaga attacccacg ttatctcagc attttactat caaaaaaatt
    23821 tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc tgaagctcaa
    23881 aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg ttattatgat
    23941 ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc gattggtcta
    24001 tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca agcaatggaa
    24061 aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt aggtcatcac
    24121 ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa acatgggatt
    24181 atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt ggagacacta
    24241 gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac ctatcgctgg
    24301 catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta attctcatct
    24361 gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag cagagttaca
    24421 aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag aaaagtttct
    24481 ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg aagatgactt
    24541 aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag ctgaagcaga
    24601 gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt attttttaac
    24661 aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg cttatttgaa
    24721 agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt tggatgaacg
    24781 gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa
    24841 tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa tcaaaatgga
    24901 acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca aaatgatgag
    24961 agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg taagagaagt
    25021 agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc aatatatttt
    25081 aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc aaggggaaga
    25141 aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac agacaaaatt
    25201 tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa tccatccata
    25261 tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc tggtacagtt
    25321 gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg aaaaagaatt
    25381 aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat aagaagaaag
    25441 acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa
    25501 atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg
    25561 ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc tttaacagct
    25621 tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc aaattttttg
    25681 attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt ttcgcttgta
    25741 cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga taacttaagt
    25801 tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc agcgtgcttt
    25861 gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt ttttttcagt
    25921 aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg
    25981 tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg aatgtttggt
    26041 actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa agggatacga
    26101 ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa ccagtatcct
    26161 ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc gatatttttt
    26221 atcaacattt tttttatgac caactggttc cgacggttcc atttgccact tacttccagc
    26281 acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc tttaagcagg
    26341 ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat tggctgttct
    26401 tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt cttcctttct
    26461 tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt tgaagtggat
    26521 gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc agaagagcca
    26581 aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct aaattattac
    26641 aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc gttttaatag
    26701 gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa tagataagct
    26761 aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat gaaatacttt
    26821 aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta aattgggggt
    26881 gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa atacttgtaa
    26941 gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc ttaagaagac
    27001 aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa
    27061 tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag ctacccagcc
    27121 aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg ttgcgatagg
    27181 gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc cttgaagcca
    27241 agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag ctgccgctat
    27301 catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta aaaaaagcca
    27361 actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt ttttagaaag
    27421 ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt tcataaaata
    27481 aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc tttgtatagt
    27541 ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc
    27601 aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga
    27661 tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat cgaaaaaata
    27721 atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta gacaaattta
    27781 tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc tgtcccttgc
    27841 cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac tccatcacca
    27901 accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt tttttcttcc
    27961 ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc ctttgctgtg
    28021 ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa gtaggaaatt
    28081 acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat taactgctga
    28141 tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat catttccgca
    28201 atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac tttccattgc
    28261 gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg taagtattgg
    28321 atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc
    28381 gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag
    28441 ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct
    28501 tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc attttttgca
    28561 gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc acaaggagaa
    28621 gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca acctaggaaa
    28681 aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac ataattattt
    28741 tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc ttctactaat
    28801 tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat cgtgatggga
    28861 tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc tacaggcaca
    28921 gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa aaagccatct
    28981 atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag ttgagaaaca
    29041 tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg ttgaagattc
    29101 atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa tgcaccgctt
    29161 aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa acaggcacca
    29221 atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca caattctttc
    29281 aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata aaaaagtggg
    29341 gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa aaggcagccg
    29401 attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac
    29461 aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga acgataatgg
    29521 ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatga
//
LOCUS       DACDID010000017        28145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDID010000017 DACDID010000000
VERSION     DACDID010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.017"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(603..1547)
                     /locus_tag="IV236_000609"
     CDS             complement(603..1547)
                     /locus_tag="IV236_000609"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8770830.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1843..2787)
                     /locus_tag="IV236_000610"
     CDS             complement(1843..2787)
                     /locus_tag="IV236_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8770831.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            3008..4222
                     /locus_tag="IV236_000611"
     CDS             3008..4222
                     /locus_tag="IV236_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8770832.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4244..6259
                     /locus_tag="IV236_000612"
     CDS             4244..6259
                     /locus_tag="IV236_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8770833.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6342..7715)
                     /locus_tag="IV236_000613"
     CDS             complement(6342..7715)
                     /locus_tag="IV236_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8770834.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7741..8025)
                     /locus_tag="IV236_000614"
     CDS             complement(7741..8025)
                     /locus_tag="IV236_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8770835.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8027..10042)
                     /locus_tag="IV236_000615"
     CDS             complement(8027..10042)
                     /locus_tag="IV236_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8770836.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10221..10526)
                     /locus_tag="IV236_000616"
     CDS             complement(10221..10526)
                     /locus_tag="IV236_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8770837.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10615..11646)
                     /locus_tag="IV236_000617"
     CDS             complement(10615..11646)
                     /locus_tag="IV236_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8770838.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11853..12728
                     /locus_tag="IV236_000618"
     CDS             11853..12728
                     /locus_tag="IV236_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8770839.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12880..13722)
                     /locus_tag="IV236_000619"
     CDS             complement(12880..13722)
                     /locus_tag="IV236_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8770840.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13814..15691)
                     /locus_tag="IV236_000620"
     CDS             complement(13814..15691)
                     /locus_tag="IV236_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8770841.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15688..17109)
                     /locus_tag="IV236_000621"
     CDS             complement(15688..17109)
                     /locus_tag="IV236_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8770842.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17112..20501)
                     /locus_tag="IV236_000622"
     CDS             complement(17112..20501)
                     /locus_tag="IV236_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8770843.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20758..22149
                     /locus_tag="IV236_000623"
     CDS             20758..22149
                     /locus_tag="IV236_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8770844.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22222..22869)
                     /locus_tag="IV236_000624"
     CDS             complement(22222..22869)
                     /locus_tag="IV236_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8770845.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22877..23563)
                     /locus_tag="IV236_000625"
     CDS             complement(22877..23563)
                     /locus_tag="IV236_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770846.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23569..24642)
                     /locus_tag="IV236_000626"
     CDS             complement(23569..24642)
                     /locus_tag="IV236_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8770847.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24864..25538
                     /locus_tag="IV236_000627"
     CDS             24864..25538
                     /locus_tag="IV236_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8770848.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25543..26883
                     /locus_tag="IV236_000628"
     CDS             25543..26883
                     /locus_tag="IV236_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8770849.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27032..28018)
                     /locus_tag="IV236_000629"
     CDS             complement(27032..28018)
                     /locus_tag="IV236_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8770850.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8239 a         5281 c         5090 g         9535 t
ORIGIN      
        1 atgtttaatc atacaaaata gttttttcat aaaaatatgg aaaatatgga ttaattttga
       61 aataattgat ttaaaaggaa tacaaaagag tgaatagtag gaaatagtgg tttagaacta
      121 gtgggctgat tttttatata ttttagttta cataatatat attatacaaa gtagatttaa
      181 aaaatgataa acctctctcg aacggttttt tatcaaaaat cgtcattttt ccatcaaaat
      241 ctgcttatgc aaaattataa aaaaattttg cagtttttct tacttttttt gtcgtttaat
      301 tgaatgataa aaaccagttt ctttattcag ttaatcaaat atgaacgaat tagatatgaa
      361 tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac attgataacg
      421 attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg atacattttc
      481 ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc aataaaaaac
      541 tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat attcattgtt
      601 tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct gaacattgct
      661 ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc gtcaacgatt
      721 gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata agcagataca
      781 ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt actcatacca
      841 ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt acggacttta
      901 tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac aatttcaaac
      961 actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc accatgttca
     1021 cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc gatttcttta
     1081 cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt tggcagacca
     1141 gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac aacgataata
     1201 ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga cgcatttgtt
     1261 ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt gataacaacg
     1321 atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt ttcatcgccc
     1381 caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg attcacatcg
     1441 atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat acttgttcca
     1501 acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa taaacagctc
     1561 cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc attttatcaa
     1621 catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc tgaatacgca
     1681 taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc aaaagtttag
     1741 aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc aatcgcacct
     1801 aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt tatttctcca
     1861 ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag tcgtttgttt
     1921 atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa tcagttccaa
     1981 cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa aatacatttt
     2041 caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt cttttaacgt
     2101 acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat tttctgcgca
     2161 tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga tcagtaatag
     2221 tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat aaatcaacag
     2281 catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat gaatgaattg
     2341 tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta gattatttgt
     2401 gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata agatgttgat
     2461 taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat caatgatcac
     2521 cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac atgtaccgct
     2581 atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg catatcgacg
     2641 gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga tttttggcgg
     2701 tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt cacgctgcag
     2761 agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt tccaattgac
     2821 tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact gctattatat
     2881 gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac ttcctgtgat
     2941 tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt acaaaaatga
     3001 aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag tctctcttat
     3061 tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta cttatgccac
     3121 gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg tcactatttt
     3181 tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg
     3241 attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt acagtattgt
     3301 cttagcttca agagctggac taggtattgg ctttggattg atcaattctt tggcagtcag
     3361 catgattgct gccttttatc acggagatga acgagcctct atgattggtt ttcaaagtgc
     3421 atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag tacagttcgg
     3481 ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct tgtttgtttt
     3541 gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc caacaagaca
     3601 agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc tggtcatcgt
     3661 ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt ctctaggaaa
     3721 cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg ctgccggagt
     3781 ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt ctttactact
     3841 gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga ttgggtttgg
     3901 tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca accaagtaag
     3961 tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg ttggcgctaa
     4021 tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt ctcttgggag
     4081 taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg cgcttattag
     4141 ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg aagaaataaa
     4201 agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca
     4261 taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac tgctacaaaa
     4321 tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg atgattgcca
     4381 tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt ttaaagtgac
     4441 gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg gaaaatggct
     4501 gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat cagggctacc
     4561 agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag caagtaattt
     4621 tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa aagatccaaa
     4681 tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga ttggcggagg
     4741 aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat ctctggatga
     4801 ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta tggatatcga
     4861 ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta aacgcgcttt
     4921 tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag tttttcatga
     4981 atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac tgaatcagat
     5041 ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta tcgacggtat
     5101 caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga tcaatcaata
     5161 tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag gacaaaatga
     5221 aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga ttggtatcga
     5281 aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc ctaagatgca
     5341 atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt atgggagagc
     5401 aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc ttgctgcaga
     5461 acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata tcttagctga
     5521 aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat atcaactatt
     5581 gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta tcgattgcta
     5641 tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt
     5701 tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg acactatcac
     5761 acagattact actccatatt tcaaattcta tgaacaagat gctttatgca aactcggaca
     5821 gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat ctcatgatgc
     5881 ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt acagtatgta
     5941 tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa tctatctttt
     6001 aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat ttatgataca
     6061 acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag atggaaagat
     6121 ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag gcggaacagt
     6181 gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct taacaagcta
     6241 ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga ctttaaatct
     6301 tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg ttctttagaa
     6361 acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag taaaaagaaa
     6421 gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc aagtacaagt
     6481 cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag atctcctaat
     6541 actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc agaacctaat
     6601 accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc tccgcagaag
     6661 aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat agcaaacatg
     6721 gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt aggtgcatat
     6781 ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt tttagcaatc
     6841 ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag gacgataaca
     6901 aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt gtctccattg
     6961 cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac atatacgatc
     7021 gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact tttaggaatc
     7081 tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca tccactagaa
     7141 gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt gatgttcttc
     7201 ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa gaccccgcct
     7261 aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga
     7321 caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct tgtgaatcta
     7381 gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg tgtaccgtat
     7441 tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg agtatgcaga
     7501 gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc agcaccgccg
     7561 atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc tgaaaatttc
     7621 ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga gggctgactg
     7681 acgattgaca caaacaaatt caacaattta tccattgata gttcctccct tgaatcattt
     7741 ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt caatgatcga
     7801 ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca aagaatgctc
     7861 aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat gacttacttc
     7921 aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca tctctaccat
     7981 aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt
     8041 ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct tgaagatttt
     8101 gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc tcattatcga
     8161 tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc agatccacag
     8221 gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct ggacgagcat
     8281 gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc acgcgctcga
     8341 tcatagcttt gatataactt tcttctattt tattttctga taatagcggc tgtgcggcaa
     8401 atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt tcttctgtta
     8461 ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg ttcattttac
     8521 gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg atataaggca
     8581 gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc agccaatctc
     8641 tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga tgcagaacat
     8701 agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata atatcgtaag
     8761 aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg
     8821 ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca cttggacaga
     8881 cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg atctctccgc
     8941 caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct attcgtcttt
     9001 ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga tgcttaattt
     9061 gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag gcagggacca
     9121 agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg gccatcagga
     9181 attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt gcaggctcct
     9241 ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc tccgaatttt
     9301 gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg actgcaagag
     9361 aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt cgaatggttg
     9421 tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga agatctgata
     9481 gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg gaaagggttg
     9541 cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga gcagtgcgat
     9601 tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca gttttcctag
     9661 tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg atatctgaca
     9721 ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg
     9781 cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta tacacagctt
     9841 caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat ttctttatac
     9901 ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg
     9961 tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt gttttcagga
    10021 gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct agtatggtag
    10081 cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt acgactgtac
    10141 cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt
    10201 tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt cagttcttca
    10261 aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg attgggctca
    10321 ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc tttattttct
    10381 gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc accagaatta
    10441 gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct taatcgtttg
    10501 tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat actttttcat
    10561 cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat
    10621 ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca tttgtaagat
    10681 tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt aaatcccttc
    10741 gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat aactcccttg
    10801 ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa agtcttcatg
    10861 atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa
    10921 catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga aataactgga
    10981 ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat cattcattcg
    11041 tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc cgaaataaga
    11101 aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg cagtagaaaa
    11161 ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt ctacttctaa
    11221 aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt cagaatcgaa
    11281 ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg caaataactc
    11341 ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct taccatgttc
    11401 gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga caactaattg
    11461 gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt cttcgaatgt
    11521 tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt tcgtaagttt
    11581 tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat aagcgattgt
    11641 aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa agcgcagaaa
    11701 gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat cattcggttt
    11761 ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg aaccaagtgt
    11821 ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa ctaacaaaac
    11881 aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg aatatcgaag
    11941 ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc cattcagcta
    12001 tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt ttcgagctgg
    12061 ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct aattttcctt
    12121 tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta tcgatccagt
    12181 caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc ggtaaagtat
    12241 tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc acggcaagga
    12301 aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa gaatacagtg
    12361 attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt attagctaca
    12421 gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta gaggccccta
    12481 ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat atgtgtcttg
    12541 ttatatcgca ggaggaatat gattttgcta aagtagcgac tttctcctct caaattgcct
    12601 tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt gaagagaata
    12661 tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa gaatatcgga
    12721 aaacttaaga aaagatcctc atttagtaat aagaaagata gtgtttgata ggctctcttt
    12781 gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg tttgtttacg
    12841 gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt ttcttatttt
    12901 gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg cagctaacaa
    12961 tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt gaacttcttt
    13021 tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga ccatataagg
    13081 tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat cttttgtatc
    13141 agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc cattgatttc
    13201 gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact ttgcttgagg
    13261 cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag gataagacgt
    13321 tccttccaat aatgaggcac ctttttctag cagcacagta ttcgtttcat caaaaattgg
    13381 caaatggaca ttgatctcag ggatcgtcaa aataccgatc gtatgtttct caaaataggt
    13441 tctatccttt ttcgcaggtt cttttttctt agtaaaggga tctgcacctg gatttccacc
    13501 ttcttttgct aattgctggt ttttctttgt catgttttct tggattttag ccatgacttc
    13561 ttcattttct ttgaccgctt gttgctgata atgagagatg atctgctgat ccagataatt
    13621 attcagcgcg tcgctaacaa atggataagc tagtatccct attcctgaga gaagcaagaa
    13681 aagcatcagc agatctagaa gtttcctttt ctttggtttc atttgtgttc tccttgtgat
    13741 gactgtttga taataaaaga aaaaggagca tttgcgtaat ccgctgctcc tttttccaat
    13801 tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc aaagtacagt ccagcaatca
    13861 gtagaaggac tactcctgca ccgatataca catagattcc cttaccgcct gttgaaggaa
    13921 gtgtaccttt gtgtttattt ggtatttttt caggagaaat caactgtcct gctgttacat
    13981 aagattgttc atcgatagta aatgctacac ggtttgttaa tggaacatat ttttctggcg
    14041 ctttcgtttc ttccagatag tacgtgccat attttagacc tgtcacatcg attaaaccgt
    14101 tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga tacccagctg acggcttttg
    14161 tggatgggtc gatcgataaa tatttcgctg tgtcactatc ttgatcacga acgacgaatt
    14221 ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca aatcgtttgc
    14281 ccccagtaac gacatcgact gacggtggtg tttgatcatt tgtatgcccg ttatcgacat
    14341 ttgcttggtt agaaaatcct ttggttggat ctgctttttc gttcaaatgc atatagtaaa
    14401 cgaatttcaa tgtaccgcca ggagttaatg aaggaatata attcggatca actgaaaccg
    14461 tgaatccgtc tgtttgctca gtgacagaat aatttactgg gtcgatttct ttatttccat
    14521 catataaggc ataagcatac gttcctgaag aatcattatc aaatgttaaa gcagcgtcat
    14581 gagtatcgat aagtttgaat gttgtgtact tgttttgcgt tccttcttta tcagcgatac
    14641 ctaatgggat attgacagaa atctcatatt gaattttttc accgattgcg acatcttttc
    14701 cattcaattg aggtgttgtt ttatcaactt tagacgtatc atttttgatg gtcttttcta
    14761 ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt tcaatcattt
    14821 ccgcattatc aggagctttt acttcttcta aattatattt tccaacttct aaatgattaa
    14881 cgatcaactg gcctttttca atagatgcct cggcaaagtc attgttgccg atgtcataag
    14941 aatgaccagt aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg tacaatccat
    15001 ctgtgacact ttggatgtat tttttgacgc ttggtgttcc ttcttcttta gaaataataa
    15061 attctgctcc atttagtgct tcgttttcgg cagtaccgat ttttgtaact ttcaacgttc
    15121 catcattacc gactgtattt ttagggtaga gatggatagt atctagttct tctgtcccgt
    15181 atttataaga gccatctgct tgtttgatca tctcatatac agggaaagct aaaaccatgt
    15241 ttgcggcagc tgacactccg tcttttggtt cttctttgat cgtgtagact gcatctttcc
    15301 cattttgttt tttaggtaaa gataaagtga catttccatc agcatctgtc gttcctgaag
    15361 caactggtgt accaggagtc aaagactgga ctgcttgttt tgctgcatcc acggacgctc
    15421 ctttatctcg ttgcgcataa aattcttgag tgacgttata aactgaaaat gaaatatcgg
    15481 ctaatccttg gtattgatcg aattcgctca tttctttccc gctgttttgg attaaaggat
    15541 cgggtaaatc agtcattttc tttttatgaa ggatgacttg agctgctgtc tcctctgcaa
    15601 acactttttt tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa aggactccta
    15661 acataacgtt tattttttta tgatttttca ttttttcact cctgttcttt ttttgataaa
    15721 caaccagatt gcaaagcttg cgaataccat accaacaacg cctaaaagtg tatttttcgt
    15781 ttcgtttgtt tttggaagaa atggacggct attttctttt ttattgacta ctttcaatat
    15841 actgtccgcc tctttatcct cagaaagaaa agttgaaatg gtaaacggaa tttctgtttc
    15901 acttaaaata taaccagaag gtgcttcgat ctcttttaat cgataacttc ctgttttcaa
    15961 cccggaaatt gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat ctgattcgtt
    16021 ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt tgtttttaat
    16081 aaggaatact gcgccagcta atcgtttatc tggtttattt gtctcgattt tgacaaattg
    16141 cttactccct gtatatacag agactttctt tgtactgaca ccaaaaccaa aatccagtgt
    16201 tccgttgttg ttgatttcct tgttcgcctg tgctgtactg cttagctgca tctgataaga
    16261 aatggtcagt ttttttccag catatttttc caacgaaggg atcgaaaata aaacatcaaa
    16321 tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat tcgatgaaat
    16381 cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca attcgaactt
    16441 ttgatagtca aggatattta caggaattgt cgtatgcaag gaataagtga ttttttcacc
    16501 aatcgtaaaa tcatttctag gctcttctaa taccttctca aaaggtggat cagggtagtc
    16561 gttttcttca tttttaggat acaaatggat agttgaaagt ttttgattgt tttgtccatg
    16621 tacaggaaga acaactacca tattctctgc cttttctttg acgacttctg gtactttgga
    16681 ttcaatgaaa agatacgctt tatctctttt ttcttgatct ttagcggcca gagaaaaaga
    16741 agcgatacca tcttcgttgt taagagttgt agttgttgct tctgcggtaa acataccgga
    16801 agacgcaccg ttttttgcga tctctgcttg tgcttcttct accgttttgc ccttttcccg
    16861 tagatggtaa aaagaatctg tgacatcata aacttggaat gtgacaccat ttaatcctcg
    16921 atacgtttgt aataaagctt tttcttgtcc gtcatttgga tgatttttcg gcagttgccc
    16981 gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg tttctgttgc
    17041 tacctgagta aaagctggtt taaatagtaa caagaagaga cacatactaa gccaaccaag
    17101 ttttttcata tttagctgcc ccctctttct tttttcaaaa aaagatacca aatagaaaac
    17161 gcacaaccaa tcatccctac tagatagatt cctaaacggc caattcctcc cgtttcaggt
    17221 aatggtactt ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt ggctcctggt
    17281 gacagaacag attcatgatc ttgtccatcc acttgaattg acccatcttc gtgtataact
    17341 atagtaactg gctcttttag accttggtat ccttctggtg taaaagtttc ggtcagcgta
    17401 taagttccag gttttaaatt ttcgaataga aaggtatctg tttcttgtcc atcttttggc
    17461 gattctaagt ccatttctgg tccctgcagt ctgaattttg ctccttttaa tgtctgtccg
    17521 ttgtcttctt ttttgtgtac cgttagatca aacgctttta gtcgattttg atgagtgagc
    17581 gtccatacat tatttgtcat ctcggtattt tttcccgtgt aaatgatttt tccatattta
    17641 tcgatttgga attcataatt tcccggcgaa gtatcatatc cggcaggacc agctgtttca
    17701 acgactcgat atttaccagg tgtgagtgaa tcaaaactgg caagaccttt ttcattcgtt
    17761 gtttggctgt caattggctg ataagtacca tttgcttctt ttttctgtag ggaaaatgct
    17821 gctcctttaa gatttatctc tttgtccgtc ccttgcatcg catacttacg tactgctaca
    17881 ggaacttcaa caaaaggatt ttcaatagtc aactggatcg tatcatcgga agtagtgact
    17941 gtttttccat caatggttac cgtaccatca gaagcgatct tgatttccca agtagtcttt
    18001 ttgcttaatc catgcccttc tggagctttt gtttctgtca gcgtataggt catttctttt
    18061 tgcaatttga cattttctgg aagagaatag gttccgtcgc catgatctgt tagtaaagta
    18121 tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa gatccttttc acctgtagag
    18181 gaatttttcg ttattttcaa gtttaacggt gtgaattgtt tagtattttt ccatgtcatt
    18241 gcatctattt gttgagaatc gtatcctggt acaggtaatt ctttgattgt ttggtaactg
    18301 aatgcttgac cttgattatt gtattgaggt agtgataata tctcttgata actttctccg
    18361 ctatttgcag ataatttgtc aatgtctgca cgttcccacg tatttgttgt atctttagct
    18421 ggtttaatga ttcgaatata tccgtttttc caagctgcag catttgtcgt atgttcccgt
    18481 tgaatctcaa aagtaacttg atctggacga tcagctagat tgttgtcaaa ttcttcccat
    18541 aactttttga tgtgaagact gactccggga gctttagcag atggtatacc gaattcagct
    18601 aattcattgg tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg ataccagaaa
    18661 tttggatgga agtcctcatt ttctgtttgg attctcactt ggtaatggat ttggatttct
    18721 tggtcttttc ctaaatagat ctgattgctc ttgatggtat ttccttctat ggaaatagat
    18781 ggagtgaccg ttgtaggact tgtccccaca ctcttgactg aaagtgtacc aggctgataa
    18841 atgaatggtt ctgcgattgg atcatttatt tgtccattgc ttacagttgc tgagatctgt
    18901 acagcttttt tggctaggta ttcagagata tctgttgcat gatcagctga ttcatagtaa
    18961 agatcgcctt tttcatctga tgaaaccatt tgtcgcatac gagactctac ttctgctttt
    19021 gagagaccag ctgccggatc gctttgaagt tggatgccaa gaccatgtat ttcgattcct
    19081 cgttctttga gtgccatcgc ttctccgatg gttgcgataa acgtactgtc gattcttctg
    19141 gatagattgt tttggtcagg tgcatcatag attcttgaga ttagagaagt atttcccggc
    19201 cgatcttgcg tattagaaaa ctgagttccg taataattgc tgcttgattg tgcgtgtacc
    19261 cgctgtactt tataggaaaa tgttggtaca ccatccgtca gcaaaacgat cactttttta
    19321 tgtccatttg gaacggatag catgcttcct gcatctctta gtgctttttg tgtaaaagta
    19381 ccaccatttg tccgtgaagg tgtaatggat tttacttgat ttttcactga atcaaatgaa
    19441 cccatctgta ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc gacatatccc
    19501 atattgattt tgtctgtgat accgctatct gctaaagtat cgacaaaacg atcgacacca
    19561 atctttactt caccgatccg attattgtcg ttcatacttc ctgaccagtc tacgaccaat
    19621 acgagatcaa gaggcgtgat atctttttgt acatttccac gagcattcaa ataaacatca
    19681 aacaatccgg gtgtacttgt ttcctttgca tactttcgta tcgcatagtc tgcttgatta
    19741 ccggttcctc catattcgat atacgaatgg gtccgatcat taggatctcc attccaagaa
    19801 tttataccat cccattgatt tgttccgttt ttattgcctt gatgatcaag aacatttgta
    19861 tttcctgtag gttgccaact ggctttagga taagtgccct gatcatctgt tgtatattct
    19921 gtaggaatcg tttgattaga tggcacactc acagctggca aggataatgt aggcaatgta
    19981 aatgggctga cagaattttc gttttctttt gatgattctt cttctatttc gtctggcttt
    20041 ggtactgaca catctgtatc tttcttattt tctgtctcat caataatgtc atattcttta
    20101 ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat tggaaaactc
    20161 agatcgatag tttgtttagt gtttgatgag atttccaacg taattttccc gctatttacc
    20221 tgataagcat agccatctcc ttcttttctt tccacatcac ttaagtaatt ttcgactaga
    20281 ccaatttctt tttctatctt ttcagcactg gtgttattcg catttagctt gatgttcagt
    20341 gctttttttc cagactgatc cgaaatgact tcagctgaaa ttgtttctaa aactgttgga
    20401 tcatctgtag tgtctgctat tgcagataat ggcacaaagt ttgataaact caatattatc
    20461 agcatgaaaa taactttcag tttcttccct gtggtcgtca tttttcccct cctttttctt
    20521 tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt acataatttt
    20581 atttatttta atatgataat tattatacga agggcgctaa agggaatcac accaattttc
    20641 ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt tcattgaaag
    20701 aaaagggttg agggagaaaa acatatgatc aggttgttgg aatgaaacta tagaaagatg
    20761 gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa agaacttgcc
    20821 aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat caaaatccgc
    20881 tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg
    20941 aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt
    21001 ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt
    21061 tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc
    21121 tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc
    21181 cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca
    21241 tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat
    21301 ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat
    21361 ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag
    21421 ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag
    21481 tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg
    21541 gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt
    21601 tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt
    21661 tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac
    21721 tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt
    21781 caattttatt ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt
    21841 tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt
    21901 atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt
    21961 gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac
    22021 aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct
    22081 catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc
    22141 gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca
    22201 aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg
    22261 aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt
    22321 cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga
    22381 gaaatgattg attcttaata attccagcca tttttcgttc attggagaat cagccgatag
    22441 ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca
    22501 atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa
    22561 gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact
    22621 gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc
    22681 gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga
    22741 gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa
    22801 aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt
    22861 cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca
    22921 ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg
    22981 ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg
    23041 tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc
    23101 gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa
    23161 gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg
    23221 aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag
    23281 agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa
    23341 gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc
    23401 ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt
    23461 ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac
    23521 caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat
    23581 tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc
    23641 gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat
    23701 cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac
    23761 caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc
    23821 ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat
    23881 accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt
    23941 ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc
    24001 cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg
    24061 ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag
    24121 ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt
    24181 caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc
    24241 cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt
    24301 tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc
    24361 tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag
    24421 atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat
    24481 ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag
    24541 tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga tcaagccata
    24601 tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg
    24661 atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg
    24721 tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt
    24781 aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt
    24841 gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat
    24901 tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg
    24961 gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga
    25021 tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga
    25081 ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac
    25141 gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga
    25201 aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata
    25261 tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag
    25321 gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag
    25381 agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag
    25441 tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa
    25501 ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca
    25561 cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt
    25621 cttttgggat tcattacttt acagatatta aaccaatctg catacagaga aacagatctg
    25681 tcattagaga aaataaattt tgattctcga acagtacaat tagagataca aagatatcag
    25741 caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag
    25801 ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat
    25861 ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa
    25921 atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca
    25981 tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc
    26041 tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg
    26101 attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg
    26161 tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag
    26221 aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt
    26281 tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca
    26341 gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct
    26401 gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt
    26461 aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca
    26521 acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat
    26581 aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat
    26641 aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca
    26701 aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac
    26761 gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta
    26821 tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa
    26881 taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc
    26941 gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga
    27001 ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt
    27061 gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt
    27121 caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc
    27181 ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt
    27241 ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt
    27301 tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc
    27361 cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat
    27421 catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct
    27481 cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt
    27541 gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag
    27601 tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt
    27661 ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat
    27721 attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa
    27781 aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat
    27841 ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag
    27901 tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg
    27961 ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt
    28021 aaattcctcc tagcaaatag atttatgatt ggtatattgt tacttataaa tttattctat
    28081 aggagacgat tgttttctac aaatatttag attggaattt gattaaaaca atttaattga
    28141 aattt
//
LOCUS       DACDID010000018        27437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDID010000018 DACDID010000000
VERSION     DACDID010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.018"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IV236_000630"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IV236_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IV236_000631"
     CDS             complement(407..766)
                     /locus_tag="IV236_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8770851.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IV236_000632"
     CDS             complement(796..1137)
                     /locus_tag="IV236_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770852.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IV236_000633"
     CDS             complement(1134..1808)
                     /locus_tag="IV236_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8770853.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV236_000634"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IV236_000634"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8770854.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IV236_000635"
     CDS             complement(4322..5512)
                     /locus_tag="IV236_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8770855.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IV236_000636"
     CDS             complement(5533..6060)
                     /locus_tag="IV236_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8770856.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IV236_000637"
     CDS             complement(6107..8896)
                     /locus_tag="IV236_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8770857.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IV236_000638"
     CDS             complement(9043..9243)
                     /locus_tag="IV236_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8770858.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IV236_000639"
     CDS             complement(9696..10016)
                     /locus_tag="IV236_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770859.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IV236_000640"
     CDS             complement(10519..11637)
                     /locus_tag="IV236_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770860.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IV236_000641"
     CDS             12108..13415
                     /locus_tag="IV236_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8770861.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IV236_000642"
     CDS             13536..14735
                     /locus_tag="IV236_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8770862.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IV236_000643"
     CDS             complement(14762..16030)
                     /locus_tag="IV236_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8770863.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IV236_000644"
     CDS             complement(16200..16841)
                     /locus_tag="IV236_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8770864.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IV236_000645"
     CDS             complement(17250..17753)
                     /locus_tag="IV236_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8770865.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IV236_000646"
     CDS             complement(17876..18640)
                     /locus_tag="IV236_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8770866.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IV236_000647"
     CDS             18748..19023
                     /locus_tag="IV236_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8770867.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IV236_000648"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IV236_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8770868.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IV236_000649"
     CDS             complement(20579..20980)
                     /locus_tag="IV236_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8770869.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IV236_000650"
     CDS             complement(20994..21803)
                     /locus_tag="IV236_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8770870.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IV236_000651"
     CDS             complement(21790..22680)
                     /locus_tag="IV236_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8770871.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IV236_000652"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="IV236_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="IV236_000653"
     CDS             complement(23348..24289)
                     /locus_tag="IV236_000653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP8770872.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV236_000654"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IV236_000654"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8770873.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IV236_000655"
     CDS             complement(25274..26470)
                     /locus_tag="IV236_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8770874.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IV236_000656"
     CDS             complement(26488..27216)
                     /locus_tag="IV236_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8770875.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         7959 a         5866 c         4770 g         8842 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcga
//
LOCUS       DACDID010000019        26922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDID010000019 DACDID010000000
VERSION     DACDID010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.019"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IV236_000657"
     CDS             complement(152..2305)
                     /locus_tag="IV236_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8770876.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IV236_000658"
     CDS             complement(2599..3447)
                     /locus_tag="IV236_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8770877.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV236_000659"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV236_000659"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8770878.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IV236_000660"
     CDS             complement(5948..6127)
                     /locus_tag="IV236_000660"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8770879.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV236_000661"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV236_000661"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8770880.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="IV236_000662"
     CDS             8275..10554
                     /locus_tag="IV236_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8770881.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="IV236_000663"
     CDS             complement(10647..11294)
                     /locus_tag="IV236_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770882.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="IV236_000664"
     CDS             complement(11473..12192)
                     /locus_tag="IV236_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8770883.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="IV236_000665"
     CDS             complement(12323..13474)
                     /locus_tag="IV236_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8770884.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="IV236_000666"
     CDS             complement(13691..14431)
                     /locus_tag="IV236_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8770885.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV236_000667"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV236_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8770886.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /locus_tag="IV236_000668"
     CDS             complement(14815..15408)
                     /locus_tag="IV236_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8770887.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16039)
                     /locus_tag="IV236_000669"
     CDS             complement(15392..16039)
                     /locus_tag="IV236_000669"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8770888.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV236_000670"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV236_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8770889.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV236_000671"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV236_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8770890.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="IV236_000672"
     CDS             complement(17485..18012)
                     /locus_tag="IV236_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8770891.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="IV236_000673"
     CDS             complement(18192..18977)
                     /locus_tag="IV236_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8770892.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="IV236_000674"
     CDS             complement(18970..19839)
                     /locus_tag="IV236_000674"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8770893.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="IV236_000675"
     CDS             complement(19840..21219)
                     /locus_tag="IV236_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8770894.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV236_000676"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV236_000676"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8770895.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV236_000677"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV236_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8770896.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV236_000678"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV236_000678"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8770897.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV236_000679"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV236_000679"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8770898.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV236_000680"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV236_000680"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8770899.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="IV236_000681"
     CDS             complement(25177..25401)
                     /locus_tag="IV236_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8770900.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="IV236_000682"
     CDS             complement(25454..26416)
                     /locus_tag="IV236_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8770901.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="IV236_000683"
     CDS             complement(26426..26866)
                     /locus_tag="IV236_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8770902.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7345 a         5980 c         4686 g         8911 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat at
//
LOCUS       DACDID010000020        25323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDID010000020 DACDID010000000
VERSION     DACDID010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.020"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     regulatory      6..103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            219..800
                     /locus_tag="IV236_000684"
     CDS             219..800
                     /locus_tag="IV236_000684"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8770903.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            803..2110
                     /locus_tag="IV236_000685"
     CDS             803..2110
                     /locus_tag="IV236_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8770904.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2403..2891
                     /gene="purE"
                     /locus_tag="IV236_000686"
     CDS             2403..2891
                     /gene="purE"
                     /locus_tag="IV236_000686"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8770905.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2875..4008
                     /gene="purK"
                     /locus_tag="IV236_000687"
     CDS             2875..4008
                     /gene="purK"
                     /locus_tag="IV236_000687"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8770906.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4021..5316
                     /gene="purB"
                     /locus_tag="IV236_000688"
     CDS             4021..5316
                     /gene="purB"
                     /locus_tag="IV236_000688"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8770907.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5496..5873)
                     /locus_tag="IV236_000689"
     CDS             complement(5496..5873)
                     /locus_tag="IV236_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770908.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6129..6857
                     /locus_tag="IV236_000690"
     CDS             6129..6857
                     /locus_tag="IV236_000690"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8770909.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6857..7111
                     /gene="purS"
                     /locus_tag="IV236_000691"
     CDS             6857..7111
                     /gene="purS"
                     /locus_tag="IV236_000691"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8770910.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7113..7784
                     /gene="purQ"
                     /locus_tag="IV236_000692"
     CDS             7113..7784
                     /gene="purQ"
                     /locus_tag="IV236_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8770911.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7785..10007
                     /gene="purL"
                     /locus_tag="IV236_000693"
     CDS             7785..10007
                     /gene="purL"
                     /locus_tag="IV236_000693"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8770912.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            9992..11431
                     /locus_tag="IV236_000694"
     CDS             9992..11431
                     /locus_tag="IV236_000694"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8770913.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11463..12506
                     /locus_tag="IV236_000695"
     CDS             11463..12506
                     /locus_tag="IV236_000695"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8770914.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12503..13081
                     /gene="purN"
                     /locus_tag="IV236_000696"
     CDS             12503..13081
                     /gene="purN"
                     /locus_tag="IV236_000696"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8770915.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13078..14613
                     /gene="purH"
                     /locus_tag="IV236_000697"
     CDS             13078..14613
                     /gene="purH"
                     /locus_tag="IV236_000697"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8770916.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14629..15903
                     /gene="purD"
                     /locus_tag="IV236_000698"
     CDS             14629..15903
                     /gene="purD"
                     /locus_tag="IV236_000698"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8770917.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16025..16939
                     /locus_tag="IV236_000699"
     CDS             16025..16939
                     /locus_tag="IV236_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8770918.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17072..18637)
                     /locus_tag="IV236_000700"
     CDS             complement(17072..18637)
                     /locus_tag="IV236_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8770919.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18630..20081)
                     /locus_tag="IV236_000701"
     CDS             complement(18630..20081)
                     /locus_tag="IV236_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8770920.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20095..21111)
                     /locus_tag="IV236_000702"
     CDS             complement(20095..21111)
                     /locus_tag="IV236_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8770921.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21331..22419
                     /locus_tag="IV236_000703"
     CDS             21331..22419
                     /locus_tag="IV236_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8770922.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22473..23999
                     /locus_tag="IV236_000704"
     CDS             22473..23999
                     /locus_tag="IV236_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8770923.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24001..25206
                     /locus_tag="IV236_000705"
     CDS             24001..25206
                     /locus_tag="IV236_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8770924.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         8044 a         4312 c         5797 g         7170 t
ORIGIN      
        1 actattatta gttttacata cctacttata tatcgtcata atatggatga cagtttctag
       61 ccagtaccgt aaatgctgga ctataagtaa aagattggct atttcaccct tcttttctta
      121 tgataaagtg gagggtgttt ttctttaaca gaaacatagt aggtttttgc acaaaagcgg
      181 ttatttcggt attaataaat gataaaggag caaataaagt gaaagaatta gttgaacgta
      241 ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc
      301 accaagtaga ccctgtattg atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag
      361 aacaaggaat cacgaaagtt gtgaccattg aagcttctgg gatcgcacca gctttatttg
      421 ctgcaagaga attagatgtt ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg
      481 atgaagaatt attgacagct tctgtttatt cttttacgaa acaagtgaca agtacgattt
      541 ctatttctcg taaattttta tcttcagaag acaaagtatt gattatcgac gatttcttgg
      601 caaacggaca agcggcaaaa ggattgatcg aattatgcca gcaagctgga gcgcaagtag
      661 aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa
      721 tgggattgag agtcgtttct cttgcgcgta ttgcttcctt gacgaatgga caagttgagt
      781 ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt
      841 agggctgcag catctgctag cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg
      901 tacaggacta ggctttaatc aagaacagat gacttatttg atttcaattg atatctttat
      961 gtgtggatta gcgacattat tgcaattgac agttaaccga tttttcggaa ttggcttgcc
     1021 tgtcgtttta ggatgtgcga tccaagcagt agctccactg atcttgatcg gaaccaatga
     1081 aggagtagga gctatctacg gatctattat cgcttctggg atctttgttg tgttagtttc
     1141 tggtgtcttt tctaaaatta aaaaactatt ccctcctata gtaacaggaa cggttatcac
     1201 cgttattggt ttgactttga ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac
     1261 agcagctgat tttggtgaca aaacaaatct gctacttgca tttgtgacga ttctcctaat
     1321 tgtaggcgtg caaatgctgg caaaagggtt cattcgttca atcgcggtat tgattggttt
     1381 agtcggagga agcatcttag ctgctttcat gggaatgatt gatctgagcg cgattggaga
     1441 tgcgccgttg ttccatgttc cacaaccctt ttattttggt aaaccaactt tcgacgtatg
     1501 gtcgatttta ctaatgatca tcatttccat ggtcagcatg gtagaatcaa caggtgtata
     1561 ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa gaagaattga aaaagggata
     1621 tcgtgcagaa ggacttgccg ttatcttagg cggtattttc aatacattcc cttatactgg
     1681 cttttctcaa aatgtcggat tggttcaact ttcgggaatc aaaacaagaa aacccatcta
     1741 tttctctgca ttcttcttga ttgttttagg attattccct aaaatcggtg cgttagcaca
     1801 aatcatccct gagccggtac ttggtggagg aatgttagtc atgttcggga tggtggcagt
     1861 ccaaggaatc cgtatgctgt tggaagttga tttcagcaat gataaaaatc tattgattgt
     1921 tgctgtttct gttggatttg ggctgggttt caatgttacg ccgacattat tcagtcaaat
     1981 gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta atgagtagca ttacggctat
     2041 catattgaat ttgatcttta atggattgaa aaaagatgaa gaaatattgg aagaagcctt
     2101 caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg ttttgacctg attgttagag
     2161 aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt ttgagctaat accgagggtc
     2221 agctgattga acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct
     2281 ttttttcatc tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt
     2341 tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa
     2401 caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag
     2461 aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc
     2521 atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa
     2581 tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa
     2641 ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc
     2701 tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg
     2761 gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg
     2821 aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc
     2881 aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta
     2941 ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct
     3001 gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac
     3061 acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac
     3121 gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac
     3181 ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc
     3241 gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg
     3301 atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat
     3361 gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt
     3421 gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt
     3481 caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat
     3541 gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc
     3601 cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca
     3661 aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat
     3721 tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg
     3781 ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa
     3841 caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat
     3901 ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac
     3961 atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac
     4021 atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac
     4081 cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc
     4141 ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg
     4201 gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca
     4261 ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact
     4321 gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt
     4381 acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca
     4441 cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa
     4501 atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc
     4561 tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa
     4621 caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct
     4681 gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata
     4741 cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa
     4801 ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta
     4861 gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa
     4921 agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg
     4981 aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac
     5041 atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa
     5101 ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg
     5161 tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca
     5221 tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac
     5281 gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa
     5341 ttaaaataga atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg ttctgcttct
     5401 tttagtttat tatattttct tatcaataat aggaacctga ttttaaaagc ggctacctct
     5461 ttaccgtgcc tctttacata caaaaaatag aggaattaag cgtaaatggc ttggctatta
     5521 taactaaaat gctggttatg gagatgttgc agcaagtttt cgagaaaagc gtattccatc
     5581 tcttgtccat tgaataagat ctggatacga taatgatttt tgaaacgctt ctgttcatga
     5641 atctgactga ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg
     5701 cattcagcac agcgcttggt tgctcggcgg atttttcgga atgtataaaa actagtatct
     5761 ttattgcata aataaacgtc tttgatcgac agcaaatcat tatatagaca ggaacgttct
     5821 tcagctgtta attctgataa aaaaagctga ccttgtttta ttgcatcttc catcggcaag
     5881 tgcctcctcg aacccattat ccaaattttt agttaattct aatataccat cattaagtat
     5941 aaatgtaaac gcttgttgat ggtataagat tttcgtttct gataaaacac gaacattaaa
     6001 agaatctaat gccttaaatg tacatatttt tgttgattta taaaaagatg attgctaaaa
     6061 tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag
     6121 agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta
     6181 cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg
     6241 cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt
     6301 tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg
     6361 aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag
     6421 caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca
     6481 ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct
     6541 atttcttaca acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta
     6601 ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc
     6661 tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt
     6721 gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc
     6781 taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag
     6841 aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt
     6901 agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga
     6961 agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga
     7021 tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg
     7081 atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag
     7141 gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg
     7201 aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag
     7261 gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg
     7321 aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc
     7381 agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt
     7441 ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg
     7501 aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg
     7561 aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca
     7621 tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa
     7681 tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt
     7741 tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag
     7801 aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg
     7861 aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg
     7921 gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac
     7981 gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg
     8041 tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag
     8101 ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca
     8161 gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc
     8221 caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta
     8281 tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat
     8341 tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg
     8401 ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg
     8461 gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac
     8521 aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc
     8581 atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta
     8641 gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac
     8701 aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt
     8761 tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc
     8821 tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag
     8881 gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata
     8941 aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag
     9001 ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa
     9061 aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag
     9121 gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag
     9181 actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag
     9241 aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga
     9301 actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa
     9361 ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata
     9421 atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg
     9481 aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat
     9541 taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa
     9601 tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat
     9661 tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg
     9721 caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat
     9781 tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg
     9841 aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga
     9901 cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag
     9961 tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga
    10021 agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg
    10081 gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg
    10141 aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa
    10201 attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa
    10261 tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt
    10321 agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg
    10381 ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga
    10441 agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc
    10501 ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc
    10561 attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt
    10621 agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa
    10681 tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat
    10741 ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc
    10801 acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt
    10861 tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc
    10921 ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc
    10981 tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg
    11041 taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt
    11101 ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt
    11161 gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa
    11221 tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga
    11281 agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat
    11341 ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga
    11401 atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt
    11461 caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag
    11521 aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag
    11581 gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg
    11641 ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa
    11701 tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt
    11761 tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag
    11821 taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag
    11881 ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga
    11941 tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg
    12001 gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg
    12061 agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag
    12121 aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga
    12181 aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt
    12241 taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg
    12301 gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg
    12361 gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg
    12421 agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg
    12481 tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa
    12541 ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt
    12601 gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc
    12661 agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca
    12721 taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg
    12781 accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc
    12841 tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg
    12901 tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa
    12961 agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga
    13021 gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg
    13081 acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg
    13141 attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct
    13201 gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt
    13261 gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc
    13321 catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta
    13381 tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac
    13441 attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc
    13501 tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat
    13561 acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat
    13621 gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg
    13681 acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct
    13741 gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat
    13801 gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag
    13861 gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca
    13921 gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt
    13981 ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag
    14041 ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa
    14101 aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga
    14161 gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa
    14221 aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca
    14281 ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac
    14341 gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt
    14401 gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt
    14461 ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca
    14521 ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg
    14581 gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg
    14641 gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag
    14701 gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt
    14761 gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt
    14821 gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg
    14881 gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa
    14941 agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt
    15001 gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa
    15061 gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg
    15121 gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc
    15181 gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag
    15241 gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca
    15301 aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa
    15361 aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct
    15421 ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa
    15481 ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac
    15541 tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa
    15601 gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca
    15661 ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc
    15721 tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt
    15781 gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat
    15841 ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag
    15901 taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta
    15961 gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt
    16021 tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg
    16081 ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa
    16141 cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt
    16201 tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg
    16261 tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga
    16321 aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga
    16381 tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa
    16441 agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata
    16501 tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat
    16561 cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt
    16621 acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg
    16681 attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga
    16741 agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg
    16801 ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat
    16861 cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc
    16921 tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag
    16981 acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat
    17041 ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct
    17101 gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt
    17161 tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca
    17221 ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa
    17281 acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc
    17341 aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca
    17401 aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga
    17461 acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg
    17521 tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga
    17581 gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt
    17641 tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg
    17701 agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat
    17761 agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa
    17821 aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat
    17881 ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga
    17941 tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag
    18001 agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt
    18061 aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg
    18121 ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt
    18181 tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt
    18241 gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt
    18301 cgctactttt aatcggttta agcagataat catctgcctg tacctggatt gctcctttca
    18361 tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc
    18421 ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga
    18481 tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga
    18541 cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa
    18601 tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg
    18661 ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg
    18721 ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg
    18781 tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt
    18841 ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt
    18901 ttgcatgcgg taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca
    18961 gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct
    19021 ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa
    19081 tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac
    19141 gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt
    19201 tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct
    19261 tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa
    19321 gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt
    19381 gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg
    19441 atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca
    19501 tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg
    19561 acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag
    19621 atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc
    19681 ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg
    19741 atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg
    19801 atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg
    19861 gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa
    19921 gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc
    19981 gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt
    20041 gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata
    20101 atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat
    20161 atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc
    20221 tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag
    20281 cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt
    20341 gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta
    20401 taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc
    20461 tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc
    20521 gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga
    20581 gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg
    20641 tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt
    20701 gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc
    20761 gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga
    20821 ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc
    20881 ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt
    20941 ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga
    21001 ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat
    21061 catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca
    21121 ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg
    21181 aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa
    21241 aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact
    21301 cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg
    21361 actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca
    21421 gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc
    21481 gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa
    21541 tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg
    21601 gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa
    21661 gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat
    21721 gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt
    21781 gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc
    21841 tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca
    21901 tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct
    21961 acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat
    22021 tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc
    22081 atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact
    22141 ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg
    22201 atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt
    22261 gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc
    22321 cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc
    22381 gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta
    22441 aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag
    22501 atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac
    22561 gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg
    22621 tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc
    22681 tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag
    22741 aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag
    22801 cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata
    22861 aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc
    22921 aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc
    22981 ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga
    23041 aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg
    23101 cagatcgaat caccatttta cgggatggac agacgattga gacactagaa aatgaaatga
    23161 tcagcgaaga gcatattatt cgcgggatgg tgggcagaga tttagttgat cgttatcctg
    23221 aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc
    23281 ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga
    23341 ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg
    23401 gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag
    23461 ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat
    23521 ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata
    23581 aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc
    23641 ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg
    23701 ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt
    23761 tggatgagcc aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg
    23821 aagaaatggc agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat
    23881 tagggatgtg tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc
    23941 gagaagatgc agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag
    24001 ttggaacagg caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga
    24061 gcagctgatc tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg
    24121 tttcaattga tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg
    24181 caaaatagcc atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat
    24241 gtggatttgt cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg
    24301 gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg
    24361 atcggcgcat ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact
    24421 ttagcaggat tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta
    24481 gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa
    24541 ggaagcagcc ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt
    24601 gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca
    24661 acagctttct tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc
    24721 tttgcctctt atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca
    24781 tatgggtttt tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat
    24841 cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc
    24901 aacatgggag tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca
    24961 acacctcaag caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt
    25021 gcttcgactt ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg
    25081 ggtgtattga acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc
    25141 aaaggattga tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca
    25201 acgtaggaga acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt
    25261 tttttcgcta aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt
    25321 ttt
//
LOCUS       DACDID010000021        25315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDID010000021 DACDID010000000
VERSION     DACDID010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.021"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(242..1177)
                     /gene="murB"
                     /locus_tag="IV236_000706"
     CDS             complement(242..1177)
                     /gene="murB"
                     /locus_tag="IV236_000706"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8770925.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1250..2170)
                     /locus_tag="IV236_000707"
     CDS             complement(1250..2170)
                     /locus_tag="IV236_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8770926.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2283..3035
                     /gene="xth"
                     /locus_tag="IV236_000708"
     CDS             2283..3035
                     /gene="xth"
                     /locus_tag="IV236_000708"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8770927.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3899..4432
                     /locus_tag="IV236_000709"
     CDS             3899..4432
                     /locus_tag="IV236_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8770928.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4922..5446)
                     /locus_tag="IV236_000710"
     CDS             complement(4922..5446)
                     /locus_tag="IV236_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770929.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5439..5912)
                     /gene="tsaE"
                     /locus_tag="IV236_000711"
     CDS             complement(5439..5912)
                     /gene="tsaE"
                     /locus_tag="IV236_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8770930.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6035..7015)
                     /gene="pta"
                     /locus_tag="IV236_000712"
     CDS             complement(6035..7015)
                     /gene="pta"
                     /locus_tag="IV236_000712"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8770931.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7168..7848)
                     /locus_tag="IV236_000713"
     CDS             complement(7168..7848)
                     /locus_tag="IV236_000713"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8770932.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7974..8843
                     /locus_tag="IV236_000714"
     CDS             7974..8843
                     /locus_tag="IV236_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8770933.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8869..9384
                     /locus_tag="IV236_000715"
     CDS             8869..9384
                     /locus_tag="IV236_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770934.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9431..9994)
                     /locus_tag="IV236_000716"
     CDS             complement(9431..9994)
                     /locus_tag="IV236_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770935.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10049..10228)
                     /locus_tag="IV236_000717"
     CDS             complement(10049..10228)
                     /locus_tag="IV236_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770936.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10305..10814)
                     /locus_tag="IV236_000718"
     CDS             complement(10305..10814)
                     /locus_tag="IV236_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770937.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10910..11290)
                     /locus_tag="IV236_000719"
     CDS             complement(10910..11290)
                     /locus_tag="IV236_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8770938.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11620..12369
                     /locus_tag="IV236_000720"
     CDS             11620..12369
                     /locus_tag="IV236_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8770939.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12553..13830)
                     /locus_tag="IV236_000721"
     CDS             complement(12553..13830)
                     /locus_tag="IV236_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770940.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13909..14553)
                     /gene="pcp"
                     /locus_tag="IV236_000722"
     CDS             complement(13909..14553)
                     /gene="pcp"
                     /locus_tag="IV236_000722"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8770941.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14752..15696)
                     /locus_tag="IV236_000723"
     CDS             complement(14752..15696)
                     /locus_tag="IV236_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8770942.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15698..16384)
                     /locus_tag="IV236_000724"
     CDS             complement(15698..16384)
                     /locus_tag="IV236_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8770943.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16858..17178
                     /locus_tag="IV236_000725"
     CDS             16858..17178
                     /locus_tag="IV236_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8770944.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17332..18237
                     /locus_tag="IV236_000726"
     CDS             17332..18237
                     /locus_tag="IV236_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770945.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18234..19007
                     /locus_tag="IV236_000727"
     CDS             18234..19007
                     /locus_tag="IV236_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8770946.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19188..20612)
                     /locus_tag="IV236_000728"
     CDS             complement(19188..20612)
                     /locus_tag="IV236_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8770947.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20823..21269
                     /gene="nrdI"
                     /locus_tag="IV236_000729"
     CDS             20823..21269
                     /gene="nrdI"
                     /locus_tag="IV236_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8770948.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21334..23316)
                     /locus_tag="IV236_000730"
     CDS             complement(21334..23316)
                     /locus_tag="IV236_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770949.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23330..24226)
                     /gene="murQ"
                     /locus_tag="IV236_000731"
     CDS             complement(23330..24226)
                     /gene="murQ"
                     /locus_tag="IV236_000731"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8770950.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24229..25290)
                     /locus_tag="IV236_000732"
     CDS             complement(24229..25290)
                     /locus_tag="IV236_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8770951.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         7341 a         5276 c         4486 g         8212 t
ORIGIN      
        1 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
       61 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
      121 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
      181 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
      241 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
      301 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
      361 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
      421 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
      481 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
      541 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
      601 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
      661 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
      721 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
      781 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
      841 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
      901 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
      961 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
     1021 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
     1081 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
     1141 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
     1201 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
     1261 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
     1321 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
     1381 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
     1441 ttccatcagc ctcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
     1501 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
     1561 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
     1621 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
     1681 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
     1741 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
     1801 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
     1861 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
     1921 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
     1981 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
     2041 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
     2101 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
     2161 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
     2221 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
     2281 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
     2341 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
     2401 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
     2461 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
     2521 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
     2581 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
     2641 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
     2701 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
     2761 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
     2821 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
     2881 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
     2941 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
     3001 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
     3061 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
     3121 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
     3181 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
     3241 ttattaccgc agatacaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
     3301 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
     3361 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg tgaaaaagct
     3421 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
     3481 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc
     3541 agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat
     3601 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa
     3661 aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca
     3721 taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg
     3781 agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa
     3841 atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat
     3901 gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct
     3961 tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc
     4021 tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg
     4081 caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg
     4141 tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta
     4201 ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct
     4261 gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt
     4321 agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca
     4381 agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac
     4441 ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat
     4501 ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag
     4561 gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca
     4621 gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa
     4681 gcttttggac accgtttacc aagaggttgt gaaatcagcg ttttgacctg agtattagag
     4741 aaacaaacgg caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca
     4801 gctgattgaa caccgtttat tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc
     4861 cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt ctcactcttt tcctatttct
     4921 tctattcaat caaataactc attttatata catctaaaaa ttcacctaga tctgttcttg
     4981 cccctctagg catgaccgct tcgatttgga agcccatttt tcggtaaagg tgtacggctc
     5041 tttcattacg aacttgaacg tcaagctcca atcgaaaaag tacacccatt tccttcgccc
     5101 aactgatgat ttcttctagc ataagagtgc ctagtcccat tccccaatat tcctgcagaa
     5161 tactgatacc gacttctccc acgtgagaca aacgaaattg actgtctgcc ttgacagatg
     5221 ctgttccaat aatcgtccct tcatagattg caagcagcaa taaattatta ttgctttcgt
     5281 atatataatc caactcttct tctaaggttt ctggtggtaa caggagttct gcttcatcca
     5341 aaactaaaaa ttctgtctcc ttattaactt gtcccatcat caaaagaatg tcttttgcat
     5401 ctcctggcac tgcttcccgc aagatgatcc caacttcttc actcatgatt ttcagcccat
     5461 ttttctagaa ttcgctgttt gaaggtttca ctttgctccc cgtatgagat tagtttcaca
     5521 taccgctttt ctaaatctgt atcacttttt tctaaaatca gttccaaata atcctccggc
     5581 agtgcttctt ctaatagatt tccccattca acaacggaaa gcccatcacc ttcaaaatat
     5641 tcatctaacc ctaaatctgc tccgctagct gctacccgat aaatatccat atgatacaac
     5701 ggaatccgac cttgactata ttctctaata atcgtgtatg tcggactttt gatcatttgc
     5761 gttatcccta atccttgggc aattcctttc gtcaacgttg ttttacccgc acctagatca
     5821 ccagtcaata tcagattgtc acctggcatt gcggaatgac caattgcctc tgcgaattgt
     5881 tctgtcattg ttaatcctga tagttcgatc atttttcatc ttctcccgct cttctttctt
     5941 tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag tcaagaacag
     6001 cagctgctgt ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg
     6061 tgataatcga taatttatat acatcttctt catttgctcc acgagacaaa tcagaaaccg
     6121 gtttgttcaa gccttgtaga attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa
     6181 ttttatagcc gatatttcct gactgcaatt ctggaaatac aaagactgtt gcttgaccag
     6241 ctacttctga atctggcgct ttcagttgtg caactgaagg aacataagaa gcatcaaatt
     6301 gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt agcgatttta gttgcttcaa
     6361 ctactttgtc tacttctggc gatttcgctg agccttttgt cgagaagctc atcattgcta
     6421 cttttggttc gatatcgaac aattctgctg tttttgcaga atctacggcg atttcagcca
     6481 attcttgcgc agttggattc acgttgatcg cacaatccga aaagacatat ttttcttgat
     6541 ctcttccacg taccatgatc atcgctccgc ttgtccgact gatgccaggc tttgttttga
     6601 tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca gagaccattc
     6661 cgtctgcaat ccccatatac gtaagcatcg taccaaagta attcacatct tttaaaatct
     6721 tttctgcttg ttctttggat gcctttccat ttctacgttc aacaaaagta tccaccattt
     6781 catcccactt atcgtaatta tcagggctga tgatcgtaaa gctagatgtt ttgattcctc
     6841 tggcatgcgc tgcatttctt atttcttctt cgttccctac taaaatgggt tccacaagtt
     6901 cttctgcttt caaccgggca gctgcgccta aaatacgagc atcagtagct tccgggaaaa
     6961 cgattttgat cccacggcga acaattttaa atcttaaact atcaaacaat tccacaagat
     7021 gacctccaga cgtttttata tgtattatca tacactattc cagaaaaaaa gaacagttta
     7081 ttttatctgt atcagtcaaa aatgctggaa aaattaagtt tttcacatac aaagcgaact
     7141 gcactataac aggaaagcgc tgttttatta cactgtttca ggcagttgcc aatcaatcgg
     7201 ttcttctcct aacgcaagga gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa
     7261 gccacgactt gcagataacg gactgggatg tggagatgtt atgatgatat ggcgactctt
     7321 atcgatcatt ttgatttttt cttgtgccgg ttttccccac aagataaaca cgattggttt
     7381 ttcccgttca tttaattttt caatgatcgt gtctgttaat ctttcccaac ctttcccgcg
     7441 atgagaatac gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa ggactccctg
     7501 tttagcccat gacactagat ttccatgttg aacaggtgcg attccaagat catcataaag
     7561 ctctttatag atatttctta acgatggcgg gatctttacg ccaggttgca cagaaaaaga
     7621 taagccatga gcttgatttt ctccatgata aggatcttgt cctaaaatca ccactttaac
     7681 ttcttcataa ggagtcaatt ccaatgcttc atagatatga tacatatctg gatggatctt
     7741 ttgggacgca tattcttttt ttaaaaattc tcttagatgc agataataat cttttgaaaa
     7801 ttctgaggac aaaacttctt gccaactgtt atgaatgatt tctttcaaaa actccacctc
     7861 ctaataaaaa gaataactca cattttcttc aatcacaagg gacaatcttc accctcagca
     7921 cttgattaat atggtaaact aggaacaaga agcagaaagg aacgataatc attatgatca
     7981 aattaattgc atcagatatg gacggcacat tattagatgc ccatatgagt gtttctcctg
     8041 aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg gtcgctactg
     8101 gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga
     8161 tcactttgaa cggggcacag gtatttgata aagaaggaaa ttcattgttt accgtgtcga
     8221 ttccaaataa tcaagcaatc tctgttttag atattctaga tgctaacggt atttactatg
     8281 aagtagcaac caacgatggt ctttattctg aaaatcaagc gaaacgaatt gaaagttttg
     8341 cttccatggt ggctacccat ttacctcacc ttacgtataa aatggcgatt gccatggcct
     8401 cagctaagct ggagttattg cacatcacat acgtggacag tattcgtgct attttggaag
     8461 aaaagaatct ggaagtttta aaaattattt gttttcatac agaagggcca gctgtattag
     8521 gtccagttgg aaagcagatt tctcttttag gcgatctagc cgttacttcc tctggtcaaa
     8581 acaatatcga agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc
     8641 acgagcgtgg gatttcttta gaagaagtca tgacgattgg cgataacttc aatgatgtca
     8701 gcatgctgca agtagcagga gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag
     8761 attatgccaa atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc
     8821 gcgcgattgc agaagacttg tagttttatc tagaaaagag ggatatagat gtcagaattt
     8881 ttcattcagg aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga
     8941 cattccctgt ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat
     9001 gccatgaatg gcgatctcgt tgctcacatc aagcagataa gcctgacatt cggtacgcgt
     9061 tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt caattggccc
     9121 ggtgactttt attatattcg tagacttcac tggacagtcc aaggtgatat ctataatcat
     9181 caatatcaaa tccatcattt caatcagcaa atcatgaaaa tggataaagc aacacttttt
     9241 tcaggagatt actatgtatt ggatgtctca aatgatgaag atgctccagt ttgtatttgt
     9301 attgctgcgg ttttagatta ctggctctac cgtaaagaca aaagtcgcga taagcctgtt
     9361 tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag
     9421 gtacatcttt ttagatattc ttttgcatat gatcataaag atgaccactg atcatccgct
     9481 gaccgacaac tttgaagtca tggcgttcgt ataatttttt agcctttgga tttttttcat
     9541 ccacacttag accgatgacg gagcgattag ctttcttcgc caatttaggt aatgcttcta
     9601 ataatcgcga accgatgcct tgtcctctaa aatcttctga tacactgata gagtccaaat
     9661 accattcgtt gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca tctagctgat
     9721 atttctctaa gataggtacc agtggctgat cgatgattga ttcttcttca tctgtataac
     9781 cgtaagcaac tcctgcaact ttaccgtcta tttcttctac gatacctcgt gaataactgt
     9841 aacgataatt tggatcagtg attgcttctt ctaaaatctc taaagtcttt tgttcccctg
     9901 tctgcttcaa aaatggcagt tccatatctt ttagaatcac taagatcaat ggggcgattt
     9961 ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt ctccattcta
    10021 acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa
    10081 tgaaataacc atgagtaacg attcttgccg cttcagttcg gcaataaggg cagacgactg
    10141 gcatttcttt atcattatgt tcgactacat tattgacttc ttcgtatgca aatttttttc
    10201 cgcaattagg gcatttcatt ttttccatgt tatctcaact tcctttcatc tttactctct
    10261 tagcttactc ttatattgta aaaaagagaa gcaatatcct tcttctatgc agatatccat
    10321 acctgacggt cctgttctcc atctattaat cggactttat aataaggacg ttcatctttg
    10381 actagaagtg tccactcttt cacttttgct tttttcccgt agtagctgac tgcttccttc
    10441 atcgctgctt gcggttgttt gaactcttcc gttttttcta tatcaaaaag atgttctgcg
    10501 gccttttccg tgtcatttcg tacggttgtt ttcccagtgg atggattgac tgctatgact
    10561 tgtccttcac aaaaaaactg gtattcatat gttgcattcg taggattctc caaatctttt
    10621 tcaactgcaa aatgaatagc agatatgtta tgttcttttg tttcgttttt tacgattgct
    10681 gctgcttttt tcaatgagat ccgatattcc tggtcactaa tatcatccat gtgaaaagcg
    10741 gcattgagca gtcccgtcat accggtaatc cctattacga tgagggcagc aacaccaaac
    10801 atcatttttt tcattcttca ttctcctgtt tctttttttt aaagaatatc aagaaatgaa
    10861 cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca
    10921 gttctaatgg caatccgtct ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat
    10981 cttctctaat cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa
    11041 tatcgtctac tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt
    11101 gtggcgcatt agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt
    11161 tatagtcatc tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa
    11221 cataaaaatc acgtgatttc tgataatctg aaacaatgat ggctatatga tggatcgttg
    11281 taaggttcat atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt
    11341 tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag
    11401 gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg
    11461 aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt
    11521 ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac
    11581 agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt
    11641 ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt
    11701 ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt
    11761 atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac
    11821 ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac
    11881 tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta
    11941 agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg
    12001 tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt
    12061 tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct
    12121 ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt
    12181 gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc
    12241 gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat
    12301 aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa
    12361 cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    12421 acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    12481 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    12541 agcctctatt ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag
    12601 tgattcttct aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata
    12661 atgttttcct ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac
    12721 tactagttgc catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc
    12781 ttctcgttct ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc
    12841 gaagaaccct tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt
    12901 tggttggatg aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt
    12961 tcgttgacgt tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg
    13021 tactggcact tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt
    13081 gatcaagaat tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt
    13141 ttttatctgt cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt
    13201 ttccaatgct tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag
    13261 ccgctgagct aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa
    13321 gacaaaacag tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca
    13381 taataattcg tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac
    13441 tgtcggagag acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc
    13501 ggtttttggc gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa
    13561 tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca
    13621 atctgttgca gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg
    13681 cttatcgact agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact
    13741 gcttgtgtat tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca
    13801 ttcttcaaag acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa
    13861 gctggctact aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt
    13921 tccggcattt ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa
    13981 agaagaagca atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg
    14041 atcgggtgag aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat
    14101 caaatgaagc aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat
    14161 gtacgctggt agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt
    14221 aaagtaagcg gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt
    14281 atcagggatt cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg
    14341 cccacctgct tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt
    14401 gactacttga ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc
    14461 gctgatttca tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc
    14521 aaatggatca aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta
    14581 acatatggtt atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata
    14641 gtttaaataa cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta
    14701 caatgttaga agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa
    14761 tagaatcata taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt
    14821 gatcactccg tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc
    14881 agccatcggt gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa
    14941 cgctggattc gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt
    15001 gatggccgca aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat
    15061 gatgatgccg atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac
    15121 tgtcccaact ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat
    15181 gcttaacggc cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg
    15241 attatctcga gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc
    15301 gactaaagca cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa
    15361 agctggaata aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga
    15421 tggaagccgg ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa
    15481 gatcaagcca ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa
    15541 gaccgtccag aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct
    15601 tactccagtg taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc
    15661 gcctaataaa atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat
    15721 atttcttcgc taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg
    15781 tgatagccac tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat
    15841 ctttcagtgt tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa
    15901 agaaccagca gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag
    15961 gatggacttc taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac
    16021 gaacaaatcc agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga
    16081 tcccataaat atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag
    16141 ccgctgattc tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa
    16201 aaatcgccat cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa
    16261 tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt
    16321 ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt
    16381 ccatatattt tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa
    16441 aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt
    16501 atcacaaaaa ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa
    16561 tatatagaaa taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt
    16621 tctatgaaat tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg
    16681 gtacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
    16741 aaaagatata caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt
    16801 gaagggatga ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg
    16861 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
    16921 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct
    16981 agtttttact ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc
    17041 tggacaggga ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag
    17101 ctgaatcttt ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag
    17161 attactagcg gccactagac acttggaata agtactgatc agaaaaaata agccttttta
    17221 tttaatattc aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct
    17281 ccactcatca ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt
    17341 ctttcggtcc aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt
    17401 tttagcttat cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca
    17461 acgatcatga aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt
    17521 gaaaatgaga tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa
    17581 tacccgggta tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc
    17641 aacgatgtat ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac
    17701 aaaaaagcga aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta
    17761 ctgaaccatc cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca
    17821 acaagagatg tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc
    17881 tccagtcact tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa
    17941 ggaaagatca tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc
    18001 gttatctcta caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact
    18061 ttggaagatc ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg
    18121 atccagctat tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat
    18181 ttggaatctt ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat
    18241 tgcttgtaaa acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg
    18301 ttctattagt tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca
    18361 ttgatccagt tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca
    18421 tgattgcagc tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc
    18481 tcctttatcg aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta
    18541 tttattctgt ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc
    18601 ttttcccttc gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct
    18661 tgttcactta tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga
    18721 tgattgcttg ctttatcaat agttccggcg catccatctc agcaggaatc gtcttttatt
    18781 ttgcttcgtc aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca
    18841 aatggaatcc gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga
    18901 agcaattgac tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt
    18961 tgttcttagc attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa
    19021 aagaggctgt gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt
    19081 tattctgaaa aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt
    19141 gaataaacga caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc
    19201 atcccagtgg caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa
    19261 atcttatcta agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa
    19321 aaataaatgc ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact
    19381 agcatagccc atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct
    19441 ttccgctgat aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata
    19501 gctgctgtac caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt
    19561 aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg
    19621 ttgagttttc cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca
    19681 atcggtaaga tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg
    19741 atcacgactg ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc
    19801 gccttgatcg tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag
    19861 acgtaaataa taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt
    19921 ttttttgctc gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc
    19981 accatgatgc aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat
    20041 ataatgtcgc tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact
    20101 acaataaata aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta
    20161 gcattttcga ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc
    20221 acgatcgatg ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc
    20281 atcaacaatg ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg
    20341 ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt
    20401 aaatctggac gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt
    20461 cctacgagta cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata
    20521 ataccaaata cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa
    20581 ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt
    20641 ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga
    20701 aatataattc taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa
    20761 ttacaccacc aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat
    20821 ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag
    20881 cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag
    20941 aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta
    21001 cttatttaga cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga
    21061 tgcgtgaata tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg
    21121 gaaataaaaa cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg
    21181 gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt
    21241 atcagatttt agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca
    21301 gtttcttctt tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc
    21361 ttttgctttt gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt
    21421 taccaagacg atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc
    21481 tgctacaagc gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt
    21541 accgcctaag tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt
    21601 tgtgatccca atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga
    21661 atagatatgt ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa
    21721 aactggatca cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat
    21781 agtcacactt tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc
    21841 tgtttcagtc gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt
    21901 agcaacgaag ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc
    21961 gtcagagatc aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt
    22021 tccgatcatt ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg
    22081 gcgtcctaga ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac
    22141 tggtaaagct cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa
    22201 tgccaatgct gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt
    22261 acgaccagtt tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa
    22321 cataaccatc ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc
    22381 aagtacccag ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat
    22441 gaagaaaatt tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc
    22501 taccgcatca ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc
    22561 gaagatgaca cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt
    22621 tttcagtggt gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc
    22681 aagagttggt gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta
    22741 gctaaacatc ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc
    22801 atcaagtgtt ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc
    22861 agtaccgaca aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga
    22921 tttgatttta gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc
    22981 gtttacacgg tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag
    23041 tttttctcct aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt
    23101 cccaggtccg atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat
    23161 cgcttttaat tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt
    23221 catgcagtgg acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt
    23281 tgccagttct tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc
    23341 cttttacaaa tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg
    23401 tcaaaatcat gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag
    23461 ctgtttcgta atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt
    23521 tttcatttgt tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca
    23581 tactgatagt agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag
    23641 tagagcctgt caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac
    23701 tgatttcggc ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt
    23761 aatctaatcc accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat
    23821 cattttttgt caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg
    23881 ccccttcgac agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt
    23941 ccggttctac accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac
    24001 ttgttccggc tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag
    24061 caatcactgg ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt
    24121 ctttgttcat tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt
    24181 ggtttctgcg ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc
    24241 ttttcttata aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc
    24301 tttgataact ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa
    24361 ttggatctca cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat
    24421 acgcgtagct gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct
    24481 tcgtatgaca tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta
    24541 aaattcttca tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc
    24601 aaattgctcc tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt
    24661 acttgctaat agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt
    24721 tggcaatcct tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac
    24781 aaggagacct tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc
    24841 tgtttcggga cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc
    24901 ttctttcaat tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa
    24961 ttgatgtgac cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt
    25021 tacaccaatt gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc
    25081 ctctcctgaa atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc
    25141 gatcaatcgc ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat
    25201 accgacaaag ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga
    25261 atcgttcata aatattgaaa agccaaacat attatccctc tttttctgta tccgt
//
LOCUS       DACDID010000022        24839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDID010000022 DACDID010000000
VERSION     DACDID010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.022"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(77..445)
                     /locus_tag="IV236_000733"
     CDS             complement(77..445)
                     /locus_tag="IV236_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8770952.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            595..1077
                     /locus_tag="IV236_000734"
     CDS             595..1077
                     /locus_tag="IV236_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8770953.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1114..2124
                     /locus_tag="IV236_000735"
     CDS             1114..2124
                     /locus_tag="IV236_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8770954.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2239..2952)
                     /locus_tag="IV236_000736"
     CDS             complement(2239..2952)
                     /locus_tag="IV236_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770955.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3142..3732)
                     /locus_tag="IV236_000737"
     CDS             complement(3142..3732)
                     /locus_tag="IV236_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8770956.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4216..4443
                     /locus_tag="IV236_000738"
     CDS             4216..4443
                     /locus_tag="IV236_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8770957.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4459..5625
                     /locus_tag="IV236_000739"
     CDS             4459..5625
                     /locus_tag="IV236_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8770958.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5859..6902
                     /gene="hrcA"
                     /locus_tag="IV236_000740"
     CDS             5859..6902
                     /gene="hrcA"
                     /locus_tag="IV236_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8770959.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6996..7559
                     /gene="grpE"
                     /locus_tag="IV236_000741"
     CDS             6996..7559
                     /gene="grpE"
                     /locus_tag="IV236_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8770960.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7610..9439
                     /gene="dnaK"
                     /locus_tag="IV236_000742"
     CDS             7610..9439
                     /gene="dnaK"
                     /locus_tag="IV236_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8770961.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9590..10756
                     /gene="dnaJ"
                     /locus_tag="IV236_000743"
     CDS             9590..10756
                     /gene="dnaJ"
                     /locus_tag="IV236_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8770962.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10838..11503)
                     /locus_tag="IV236_000744"
     CDS             complement(10838..11503)
                     /locus_tag="IV236_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8770963.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11479..11652
                     /locus_tag="IV236_000745"
     CDS             11479..11652
                     /locus_tag="IV236_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8770964.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11697..12434)
                     /locus_tag="IV236_000746"
     CDS             complement(11697..12434)
                     /locus_tag="IV236_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8770965.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12436..13938)
                     /locus_tag="IV236_000747"
     CDS             complement(12436..13938)
                     /locus_tag="IV236_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8770966.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14197..16191
                     /gene="uvrB"
                     /locus_tag="IV236_000748"
     CDS             14197..16191
                     /gene="uvrB"
                     /locus_tag="IV236_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8770967.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16201..19017
                     /gene="uvrA"
                     /locus_tag="IV236_000749"
     CDS             16201..19017
                     /gene="uvrA"
                     /locus_tag="IV236_000749"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8770968.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19280..20545
                     /locus_tag="IV236_000750"
     CDS             19280..20545
                     /locus_tag="IV236_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8770969.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20549..21280
                     /locus_tag="IV236_000751"
     CDS             20549..21280
                     /locus_tag="IV236_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8770970.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21415..22299
                     /gene="rapZ"
                     /locus_tag="IV236_000752"
     CDS             21415..22299
                     /gene="rapZ"
                     /locus_tag="IV236_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8770971.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22296..23294
                     /locus_tag="IV236_000753"
     CDS             22296..23294
                     /locus_tag="IV236_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8770972.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23328..24263
                     /gene="whiA"
                     /locus_tag="IV236_000754"
     CDS             23328..24263
                     /gene="whiA"
                     /locus_tag="IV236_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8770973.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT         8204 a         4260 c         5311 g         7064 t
ORIGIN      
        1 aggataggat tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc agtaaaaggt
       61 ttattcgtat aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa
      121 atgaaatcaa ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga
      181 cgttatccgt atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg
      241 ctttttccgg tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac
      301 catggtcagt aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt
      361 ctgttatctt agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatttgat
      421 tgatgatcgg ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt
      481 taccagaaaa acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg
      541 ctatatttat atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt
      601 gacttggcaa agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc
      661 atttatacaa atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag
      721 accaaaagct tattcttaaa agataaaaaa gataataact acgtatatct gacttttact
      781 acgaaacaaa cggatttcaa agcattaggg aagatcgtcg gtaaaaggct ttctattgtt
      841 tcatcagaac gcatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt
      901 tatgaaaaac agattccgat tattattgat gaagagctct tatcaaatga aaaacttgtt
      961 tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc
     1021 atccagctac ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca
     1081 agtaaaatag tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga
     1141 aatgcaacga ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta
     1201 ctcgaatcag ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg
     1261 ctttggacgc aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat
     1321 caaatgatgc aagatgaaaa tatccaaatg atctttccga cttggggagg agaattattg
     1381 attgagattc tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat
     1441 tcagatatta gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat
     1501 ggaactaacc ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa
     1561 agtgtactga aaacaagaga aggagaatcg attctacagc aatcttctga aaagtatcaa
     1621 aaagtgtggc cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat
     1681 tggcaatcag tcagcggaga tgcagtaagg atgaagggac gattattagg tggttgtatt
     1741 gatacgatca ggcatttaat cggtactcct tatggagatg tccaatcttt taaaagaaag
     1801 tataatgatg gaaagccgat cttgtggttt ctagaaaact gtgagctatc gtcagttgat
     1861 ttaagaagat cacttgttca gatgaaactg gcaggctggt ttgatggcag ctcggggatc
     1921 atgttcggta gaagtgcggc agaggatttg ttagattata aaatgaagga tgtatacgaa
     1981 gatctgtcaa aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg
     2041 ccacaactga cattgattaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg
     2101 actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc ttggagtcta tcaaagacca
     2161 ataaatctaa atagaaaaga aagtgaaaga agctgacaaa atgctgtcag cttctttttg
     2221 tataacatat tagaaagtct aattatctat aactgtcaca tcgattaact gtttcacgtc
     2281 attgggattt cctgtaggaa tagctactac agatgcatta tcaatgattt cttgagttgt
     2341 ttctctaatg aacgctggat aagatgtttg atctgcaggc atttgccaat gtgaaggaac
     2401 tggataacta gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact
     2461 gtatttccca gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg
     2521 ggcgataggt gttcctgcag gataatgaat aacatgtaat tcgaaatcac ccgatgtatc
     2581 tctaaaagta ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga
     2641 atatcctgct aacggatcta ttgactgaga agactgtgag gaggttgtgt ctattgttga
     2701 agaagaaata tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac
     2761 tttactgctt gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt
     2821 ttcagctgaa ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt
     2881 ttctttacta ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc
     2941 taactttttc atataaaata cctcattatt tttttaatac cagtatagca taatcctgaa
     3001 tttttgaatc cgtaaaacct tgtctatgaa tataaataga gtgtatcttc tttgataaat
     3061 gtcagaaaaa gtgtgacaaa taatcatcag taaaattact tgtcacacta ttgataagga
     3121 gtatttaatt gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata
     3181 gaatccaagt ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac
     3241 tgcttgaaga atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt
     3301 acgatctttc acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc
     3361 taaccaagga aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc
     3421 taaataatca actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc
     3481 gattatttga tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa
     3541 aaggtcaact ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc
     3601 aatggaagtg acctctgatt ggatagaagc agttttgttt tgccatttct cattttggtt
     3661 tagtgtgcta gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat
     3721 cgcagttttc attttcaagc tctcctttac tatctataac aaggacaaca gttttaacct
     3781 acagacattc attaaaattg ggtagcgaca gcgaaaacaa caattcttaa gttttagaca
     3841 gagtatacag atagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt
     3901 tttgtttacc agcattataa cattttgcat acatggatga caaaagaacc aattcccact
     3961 tgcttgagta caatattaaa agaaactgga aggcaaaaag taattataga aacgaaaaat
     4021 aaatcatatc gggcactcag acatgaaaac aaccctagag atatataact aggtaaccag
     4081 tttaatgaaa gaaaaaatca tccaagaagt cgattcttgg attttttagt ggatttgttc
     4141 gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca
     4201 cttaaggagg aatctgtgaa aatagctttt catatttttg cagattttga cttaatgccg
     4261 aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg tagctccgat
     4321 gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc ggcttatttc
     4381 aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga cttttcgacg
     4441 taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg
     4501 tacattcata tcccattttg cgagcatatc tgctattact gtgattttaa taaagttttt
     4561 ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat gaaaataatg
     4621 ctagaaagtc atccgacagc gaatttagaa acactttacg tagggggcgg gacaccaact
     4681 tctttatctg ctaaacaact tgatcgactg ttatcaggtg ctagagagat cctgcctttt
     4741 caaaatggta atgagtttac tgtagaagcg aatccaggtg atttgacccg tgaaaaactt
     4801 caagtaatga aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac atttgataat
     4861 cgcttattga aaaaaattgg acgtaaacac accgcagaag atgtgtatca aactatgcaa
     4921 tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc attaccggga
     4981 caaacactag aaggatatct ggatactttg gaaaaagctt tggctttgga tctgcctcac
     5041 tattctttgt actcattgat cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa
     5101 ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa
     5161 gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct ctccggacat
     5221 gaatctcaac ataacctcat gtactggaac aatgaccact attttgggtt tggagctggt
     5281 gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca acactattta
     5341 cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac acgaaaaaat
     5401 caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac taaaaaacat
     5461 ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct aagggaatta
     5521 gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc aagagggacc
     5581 tttttgggga acaatgtgtt tgagcgcttt ttgtttgaaa aatagtttaa agaatatttg
     5641 ttttttatct gaatttaaat aaaaaaagtt caaaaataca ataaaggact attgaaacgt
     5701 tgatataact ggatttacat caattattta gaattagcac tcttgtgttt agagtgctaa
     5761 aaaatggagt ctttctcttg acaggagaag ggcttcttgc tatatttata agtggattag
     5821 cacttagaat atatgagtgc taatgagagg tgaggaagat gttaacaaag cgacaaaacg
     5881 acattttacg tctgatcatc caacactata cgagtagtgg cgtgcccgtt ggttccaaaa
     5941 cattgatgtc tgagggagtc aaggccagct cagcaacgat tcgcaatgat atgaaagcat
     6001 tagaagatga aggattgctg ttgaagactc actcttcttc tgggcggatt ccatctgctt
     6061 taggctatcg ttattacgtg gatcatttgt taaaacctgc tcgcgtagca gaagatgatt
     6121 tgctgctgat tcgtcagtct ctaggcagag aatttcatga aatcaatgag attattaagc
     6181 aatctgctga gatattatct gagctgacaa gctatacgac tttttcattg ggacctgaaa
     6241 taaaagaccg ccgactgact ggctttcgta tcgtaccctt gaataatcga cagattattg
     6301 ctatcgtcgt aacagatatg gggaatgtag aaagccaagt gttcactctt cccgaatata
     6361 tgggaagtca ggaactagaa aaaatggtac agattgtaaa cgaccgtcta gtcggagatc
     6421 ctctgatgac ggtgtaccat aaacttcgta cggagattcc gatgattctt cataaatatt
     6481 tccaaacgac agaagggatg tctcagttat ttgacaccgt tcttggtcat gtttttgaag
     6541 ataaggttta tgtcagtgga caaatgaatc tcttggatta tgagccccgt caagatcttg
     6601 atcagttcaa atcgatgttt tcttttatga aagactcaga cggacttaca caaatgatca
     6661 ctccgatgga tagcaatatt catatcaaga ttggtgctga attaggcaat gatttattgc
     6721 agaatatgag tatgattcaa gcaagttacg agatagatgg tcatggaaga ggtacaatag
     6781 ccttgttagg gcctactagt atgccttatt ctcggatgtt cggtctagtc gatgtttatc
     6841 gaagagaact agcagctaaa cttgctgatt actaccgttc gcttgacttg tcaaactcgt
     6901 aatgtgaata acgaagctat ttgcagctga tcgttctctc tttcttagta agagcaatca
     6961 aacagctgac tgaataatga aaggaagact gtatcgtgaa agaaaatcaa gaagaactag
     7021 ataaagaaat gacagatgct cagccagaac cggaaatcga tgtcgaagct gcagaagatt
     7081 cgggtatttc agaagctgaa gcggaagaat tcgaaacagc gaaattaaaa gctgagttag
     7141 aagaaatgga agataaatac ttgcgggctc gtgctgaaat cgcaaatatg gcaaatcgag
     7201 gaaaaaatga gcgtgagcaa ttacaaaaat accgttcaca agatttagcc aaaaaattac
     7261 ttccatcaat tgacaacctg gaacgtgcat tagccacaga agtgtcagat gatcaagggg
     7321 caggtttgaa aaaaggcgtg gaaatggtct tggaaagttt gcgtaacgca ctggaagaag
     7381 aaggaattga aaaaatccct gctaaaggag aagcctttga tcctaatctt catcaagcag
     7441 tacaaactgt tccagcgact gaagatacac cagccgatac gattgtagaa gtactacaag
     7501 aaggctacaa attacatgat cgcgtgttaa gaccaaccat ggtcattgtt gcacaataat
     7561 tgtgcggata taaaacttac actttcaatt aaagaaggag acgttaatta tgagtaaaat
     7621 tatcggtatt gacttaggta cgactaactc tgcagtagca gtattggaag gaaacgaagc
     7681 aaaaatcatc gctaacccag aaggaaaccg tacaacacct tctgttgtat cattcaaaaa
     7741 tggagaaatc caagtagggg aagttgcaaa acgtcaagct gtgacaaacc caaatacgat
     7801 ttcatcaatc aaacgccata tcggtgaagc aggttacaaa gtagatgtag aaggtaaatc
     7861 atatacacca caagaaatct cagcaatgat ccttcaatat atcaaaggat tcgcagaaga
     7921 ctatttaggt gaaaaagtcg aaaaagcagt tatcactgtg ccagcttact ttaatgatgc
     7981 acaacgtcaa gcaacaaaag atgctggtaa gatcgctggt ctagaagtag aacgtatcgt
     8041 caatgaacca actgcagctg ctcttgccta tggtttagat aaaacagatc gcgacgaaaa
     8101 aatcttagta ttcgaccttg gtggcggtac ttttgacgta tctatccttg aactaggaga
     8161 cggtgtcttt gatgtattgt caacagctgg cgacaaccat ctaggtgggg acgactttga
     8221 taacaaaatc atcgactaca tggtagcaga attcaaaaaa gaaaacagca ttgacttgtc
     8281 tcaagacaaa atggctttac aacgcttgaa agacgctgct gaaaaagcga aaaaagattt
     8341 atctggcgta tcaagcacac aaatcagctt gccatttatc acagcaggag aatctggacc
     8401 attgcatttg gaaatgacat tgacacgtgc aaaatttgat gaattaacag ctgacttagt
     8461 agaacgtaca aaagtaccag tacgtcaagc gctaaaagat gcaggtttat cacaatcaga
     8521 aatcgacgaa gttattttag tcggtggatc tactcgtatc cctgcagttg tagaagcagt
     8581 acgtaaagaa acaggaaaag aaccaaacaa atcagttaac cctgatgaag tagttgctat
     8641 gggtgctgct atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg ttttacttga
     8701 cgttacacca ttgtcattag gtatcgaaac aatgggtgga gtgttcacta aattgattga
     8761 ccgcaataca acgattccaa caagtaaatc acaagtattc tcaactgcgg cagataatca
     8821 accagcagta gacatccatg tattacaagg tgaacgccca atggctgctg acaacaaaac
     8881 tctaggaaga ttccaattga cagatattcc agcagctcct cgtggtattc cacaaatcga
     8941 agtaacattc gatattgata aaaacgggat tgtaaatgta tctgcgaaag atctaggtac
     9001 acaaaaagaa caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg aagaaatcga
     9061 acgtatggta aaagatgcag aagcaaatgc agaagcagat aaagctcgta aagaggaagt
     9121 tgacctacgt aatgatgtag atgcattgct attctctgtg gacaaaacat tgaaagaatt
     9181 agaaggcaaa gtggacgaag aagaagtgaa aaaagcagaa acagctcgcg atgaattgaa
     9241 agccgctgtt gaagcaaaca acatcgaaga aatgaaaact aaacgcgatg cattgaacga
     9301 aatcgttcaa aacttgactg ttaaactata cgaacaagcg gcacaacaac aagctcaaga
     9361 aaacccagaa gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt
     9421 cgatggcgac gataaataat cagttcactg aatttcgaga gaaggccaaa gttgctactt
     9481 tggctttttc ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac aaaatatgat
     9541 atgataacaa gcgacgtgaa aatgatgaga accaatagga ggaaagccta tggcaacaaa
     9601 acgtgattat tatgaagtct tagggctctc aaaaggagca tcagaagatg aaataaagaa
     9661 agcttatcgt aagctttcga aaaaatacca tcctgacatc aataaagaac ctgatgcgga
     9721 agaaaaattc aaagaagtat cagaagcata tgagatttta agtgacccgc aaaaacgcgc
     9781 tgcatatgac caatacgggc atgcaggcac agatccaaat tacggtgctg gtggagctgg
     9841 tggcggattt ggtggattcg gcggcttctc tggtggcggc ttcggtggtt ttgaagatat
     9901 cttcgactct ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc ctcgtcaagg
     9961 tgctgatttg caatatacgg tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa
    10021 agaaatcaag tataatcggg aagaaatctg tcatacttgt ggtgggaacg gtgcaaaacc
    10081 aggcacacaa ccaactactt gtcataaatg tcatggtgca ggtacgatta atgtagaacg
    10141 ccaaacacca cttggacgag tgatgagccg tcaaacttgt gatgtttgtc atgggacagg
    10201 gaaagaaatc aaagaacctt gtccaacctg tcatggctca ggacatgaga agaaagcaca
    10261 ttcagttaaa gtgaatgtac cagctggtgt agaagaagga caacaaatgc gtttagctgg
    10321 tcaaggagaa gctggagaaa acggcggacc atttggtgat ttgtatgtgg tcttccgtgt
    10381 agaagaaagt gatatctttg atcgtgaagg ttctgaaatt tactatgaat tgcctttaag
    10441 ttttgtacaa gcagctttgg gggatgaagt ccaagtacca actgtccatg gagatgtgaa
    10501 attgaagatc cctgctggaa cacagacagg tacgaacttc cgtttacgtg gaaaaggcgc
    10561 tccgaagtta cgtggtggtg caacaggcga ccaacatgtc aaagtaaaac tgatcacacc
    10621 gaaaaatcta aatgaacaac aaagagaagc attacgtgca ttcgctgaag ctggcggcat
    10681 caaagttgaa gaacagcaag aagacggttt cttcgataag tttaaagatg cttttggcgg
    10741 gaaaaagcgg aagtagtttt tctagtaaaa acgctaagtc attaataatt aaaaaccaga
    10801 cgaacaatca tatgttcgcc tggttttttt gtagttgtca tgtttgtacc aacaaaaaag
    10861 ccagctttag aatatgataa tcgtcatagt ttctaggggt ataaccagtg agagaatgga
    10921 acttgttcaa tcgatattgc aaggtgtttt tatgaataaa taattcttct gagcaatttt
    10981 tgatgcttcc gttattttta ctgaagcatg ccaaagtctt cttgaacaaa tgtacttctt
    11041 cttctgtcag gcttttcaaa atacgtttta catataatgt tcgttgatct gacgggattg
    11101 ctggtaaaac gatcccgagg tctaaatctt caaaaaagcg gatctcgttc acagattgat
    11161 ggaaagctat ccattcgcaa acttgtttgg catgaaggaa agattgcggt gtctcttgct
    11221 ctgtataaca aagcgaccca attccaaagt ttagctgtaa ctgatacatt ttattaaact
    11281 ttgtctggat tttatcaagc aatgatagca atgaggctct ggaaatccga ttgactagga
    11341 taatgatttg ttgtctatgt acagtgagca aagcctgtcc gtaataagag aagacccttt
    11401 cgagcaactc caataaagga ttgatccgtt cttgacacaa ttgctgattg tcagaaagtt
    11461 gtcccacaac tacataatat ggagaagaaa aatcaagctc aattaagtga gggtggtatc
    11521 gactatgtac ttgatatcat caatgaaccg atggtacttt cttatgcgat ggaacaggta
    11581 gagcgattgc ttgtttgtgc aggaaaacaa gcgatgcggc tgatacgatt aggtgagaaa
    11641 ataagaagat aaaaatacac ggtcccttca tggcgaccgt gcatttttgg tttttattaa
    11701 atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt ttcaaagatt
    11761 tgttgaggcg ttccatcttc taaaaagttt ccgtcgtcaa tgaacatgac gcgattggcc
    11821 acttctttag cgaaccccat ttcgtgcgta acgataacca tcgtcatacc ttgttctgct
    11881 aactttttca tgacgttcaa gacatcaccg accatttcag gatcgagtgc agaagtaggc
    11941 tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc cacacgctgt
    12001 ttttgtccac cagaaagcat ttcaggatac atatcttttt tatcttctag accaactgtc
    12061 tgcagtaatt tgattgcttt tttctctgct tcttctctag attctttctt caggtccatc
    12121 ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa gtgttggaat
    12181 accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat caagtgggcg
    12241 ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca acgcaagaat
    12301 gtactttttc ctgaacctga agggccaatg atacagacga catctccttc gttgatggat
    12361 acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg ctcaactaag
    12421 attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt ttgtcaatgc
    12481 tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac tttgtgttgt
    12541 acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg cggaaatgat
    12601 cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca tgatgcgtat
    12661 cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta aacttctgga
    12721 agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct ctgcgatata
    12781 agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa tcgtaaatcc
    12841 aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg gaattcctcg
    12901 gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg ctacactgaa
    12961 taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag ttaggagaat
    13021 tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc caagaaatgt
    13081 tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta catattcttt
    13141 aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat tgaacatatt
    13201 aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc cagattcttt
    13261 tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta ccggataatc
    13321 atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat cagcagcatc
    13381 aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt cagcactttc
    13441 ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact tgatcgaagt
    13501 atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag aaaaatcata
    13561 gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg cttgtcctga
    13621 ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt taaatccttg
    13681 cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc cctttgcgtt
    13741 ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt cttttttggg
    13801 ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt tgtcagcttg
    13861 tgcaacatga ccaaacgcaa atgtcacaag tcctgtcatt aagaaaacaa atgagtgaaa
    13921 taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta ttataggcga
    13981 aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc gagtttctaa
    14041 cattttatgt tataaaacat aacatgtata ataagataaa aaagtggcga aagtatgttc
    14101 ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt tattggtcgt
    14161 aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga tacctcccga
    14221 cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc gattaaccaa
    14281 ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc gactggaaca
    14341 gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac actgattatt
    14401 gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt tttccctaac
    14461 aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc ttatgtgcct
    14521 tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga caaattacga
    14581 cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc atccgtttca
    14641 tgtatttttg gattaggctc gccttttgag taccaaaaac aagtagtttc gattcgacag
    14701 ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca attcgaaaga
    14761 aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt ggagattttt
    14821 ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga ggtcgaacgt
    14881 atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca cgtagcaatc
    14941 ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt agcgaatatc
    15001 aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact tctggaagct
    15061 caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat gggctacacg
    15121 tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga accgccttat
    15181 acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc gcacgtaacc
    15241 atgcctcaaa tcagaggaat gtataatggt gaccgagcga gaaaacagat gctagtagat
    15301 tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga agagtttgaa
    15361 aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga acatgaacaa
    15421 acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc agtgatcgaa
    15481 gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga acgagtcgaa
    15541 aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga cctcacagat
    15601 tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa aacgctggaa
    15661 cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt tggaatcaat
    15721 ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt ggatgcggat
    15781 aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg agctgctcgg
    15841 aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat gcgtctagcg
    15901 atggatgaaa cgtcaagacg tcgtacgatt cagcagaagt acaatgaaga acatgggatc
    15961 gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa agaatcggaa
    16021 gatgatacaa aagaagcggt acaagtctcg tacgaagaaa tgacgaagga agaaaaagac
    16081 acgttattga tgaaattaga aaaagaaatg aaagatgcgg caaaagcttt ggattttgaa
    16141 acagccgcga atgtgcgaga tatgatttta gaattaaaag cttcaaagta ggaggacaac
    16201 atggctaacg ataaaattac gattcatggt gcacgtgccc ataacttgaa aaatatagat
    16261 gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc tggaaaaagt
    16321 tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga gagcctttct
    16381 gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag catcgacgga
    16441 ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg gtcgacagta
    16501 ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt aggacatccg
    16561 atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat ggcagatcaa
    16621 gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt ggtaaagaaa
    16681 aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt ccgcgttcga
    16741 gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa caaaaaacat
    16801 gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc tcgcctattt
    16861 gattctttag aggcggccct gcgattagca gacggctatg ctctggttga cgtgatcggt
    16921 gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt tactgttggc
    16981 gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc agactgtgat
    17041 ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag aacgaagaca
    17101 cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta tcctcaaatg
    17161 ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga agaattaccg
    17221 gaagagcatc aggagatcat cttgaatggg acagatgatc ttttccattt ccattatgaa
    17281 aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt gaagaatatc
    17341 gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg tctttatatg
    17401 acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc tctatctgtt
    17461 caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa agcagtagat
    17521 ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc gattttaaaa
    17581 gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct aactttgagt
    17641 cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc tacacaaatt
    17701 ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg attgcatcaa
    17761 cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg caacaccctc
    17821 gttgtagtcg aacatgatga agatacgatg tgggcagcag attatttgat tgacgtcgga
    17881 cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga agaagtagct
    17941 aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat ccctgtacca
    18001 aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc agaaaacaat
    18061 ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac aggtgtgtct
    18121 ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc gcaaaaatta
    18181 aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga aagtatcgaa
    18241 aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa tccagcgact
    18301 tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga ggcaaaaatg
    18361 cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg tgaagcttgt
    18421 cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta tgttccttgt
    18481 gaagtatgtc atggtaaacg atataattct gaaacattag aagtgcatta caaaggaaaa
    18541 agcattgctg atattttgga aatgacagta gaggatgctg tagaattctt caagcacatt
    18601 ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta tgtgacgatg
    18661 gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact tgccagtgaa
    18721 ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac gacaggactt
    18781 catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga tgctggtaac
    18841 acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta tatcattgat
    18901 ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac accagaagaa
    18961 atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat ggtataaaga
    19021 ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct tgttttatta
    19081 atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg tatagataaa
    19141 aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga ctttcctttg
    19201 attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa aacaagagat
    19261 tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta tccctatatt
    19321 attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag catacggcaa
    19381 aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta aaattgtcaa
    19441 tgtagaagtt attccaggat ttgataaaga ccctgtattt atcgagacga tgcttcgttt
    19501 ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt gtggtgatgg
    19561 atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta tctgtccata
    19621 tattgattat tctttatttg aaaggctgat caataaagta agcttttatg aagtttgtga
    19681 agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt tagtaaatgg
    19741 acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg caaacagtgt
    19801 ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag agactagaga
    19861 agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg aaatgattgt
    19921 acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt atgtagatga
    19981 agatcatcaa gttcggatga tgtgccttgg tcatccacta ttagaagatc ctacaccagc
    20041 atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct atcaaaccat
    20101 taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata tgaagtatga
    20161 tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc gtagcagttt
    20221 ctttgtgaca ttgaacggct tgaatctggc tcgttttgtc acagaagatc gagtgttcaa
    20281 caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa gattatggat
    20341 gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca aaaaactagc
    20401 tgagcaaatg atcaaagaaa atcgttacgg tactactgta ttttatgaaa aagaccgatc
    20461 aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa gattcaaaaa
    20521 gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt taggcggaat
    20581 gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga aagctacaaa
    20641 agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg atcgtacagc
    20701 ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg atattgagaa
    20761 acaaaattta ttgcgaccaa acttcattgt gttgacttgt aacaccgcac actacttttt
    20821 tgaagagtta caagcggcaa cggatattcc tatcttgcat atgccaagag aagcagcaaa
    20881 tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag aaggaagtat
    20941 gaaagctgga atctatgaac gcgaagtcaa aaatcttggt ttcgaaacag tgattcctga
    21001 tactgcgctt caagaaaaaa tcaattacct gatctatcat gagatcaagg aatcggatta
    21061 tttgaatcaa gagctctatt acgaaatctt agaggaagcg gtagagcgcc taaactgtga
    21121 aaaagtcatc ctaggttgca cagaattatc tttgatgcat gaatttgctg aagataatca
    21181 ttacccagtg atcgatgctc aatctatttt agcggatcga acgatcgaac gtgcattagc
    21241 tgaaagaagc gaggcattgg atacagcatc ggaaaagtaa aaaaattgga tttggggcaa
    21301 atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa ctggtaatga
    21361 tacgcccggg atgatataat tttattatgc acaatgtgag aggggttcgt aaatatgaca
    21421 gataatttgc aattagtcat cataacaggt atgagtggtg cagggaaaac agtagctatc
    21481 caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca
    21541 aaattctggg aattgatcaa agaatcaggt aaagtcacaa aaatggcttt aggagtggat
    21601 ttgcgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg
    21661 aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc
    21721 tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata
    21781 cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg
    21841 acaacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga
    21901 gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat
    21961 tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga
    22021 ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa
    22081 tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa
    22141 ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca
    22201 ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg
    22261 gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa
    22321 gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt
    22381 tacgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat ggtggaagca
    22441 gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag
    22501 tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg
    22561 aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga
    22621 tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac
    22681 atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtttttaaa gacggaacga
    22741 ctgcagtagg agaatctaaa atcgcgatca accgtaaaac cattgaccgt gtgtatgtct
    22801 gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg
    22861 acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag
    22921 tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta
    22981 atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt
    23041 tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc
    23101 ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact
    23161 tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgacttttta gaattgaaag
    23221 atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg
    23281 gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg
    23341 cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat
    23401 tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga
    23461 acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt
    23521 atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct
    23581 atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg
    23641 gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt
    23701 cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagtcgtt
    23761 atcatttgga aatattttct atttatgaac agcataacca agacatttgc gatatgttaa
    23821 attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcgtatttaa
    23881 aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc
    23941 gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt
    24001 gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg
    24061 aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag
    24121 aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg attccctcag
    24181 gtgcaatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa tttgcggaaa
    24241 aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc aggagatgcg
    24301 aatgtatcag ttttttttaa aagctatact tgcttttctt ccgtcaaact ataattccaa
    24361 ctttactgga gtaacataat gttgaaaggc ttctattgtt ttttaataca atcgtataaa
    24421 aagcggggag acaactgaat gaagatttta gttttataca taaacttttg cttaatatag
    24481 aagtcctgat atagatagga tcaagtattt atctataact ggagattaat taaaaactgg
    24541 aaggaactgc tcgatcaacc ttgagaactg tttttcttac ttctggtcaa gattctcgtg
    24601 gttggtggat aaggatggtt gataagttca ttggattagc tagtcgaaaa caaccaggtg
    24661 taattaatgt agaagtttta acaaggggat ggagtgattt attcagtgat aatcataagt
    24721 tcataaatag taattgggat gtatggttgg tatacgttat acctgaactt gaactatcaa
    24781 gatttggttt agaatttgag aatcgatgtt tataaaggga gggcaaaaaa tgtgctttt
//
LOCUS       DACDID010000023        24383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDID010000023 DACDID010000000
VERSION     DACDID010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.023"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..677
                     /locus_tag="IV236_000755"
     CDS             30..677
                     /locus_tag="IV236_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8770974.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            761..1705
                     /locus_tag="IV236_000756"
     CDS             761..1705
                     /locus_tag="IV236_000756"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8770975.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1840..3291
                     /locus_tag="IV236_000757"
     CDS             1840..3291
                     /locus_tag="IV236_000757"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8770976.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3312..4148
                     /locus_tag="IV236_000758"
     CDS             3312..4148
                     /locus_tag="IV236_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8770977.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4148..4807
                     /locus_tag="IV236_000759"
     CDS             4148..4807
                     /locus_tag="IV236_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8770978.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4933..5622
                     /locus_tag="IV236_000760"
     CDS             4933..5622
                     /locus_tag="IV236_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770979.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5628..6584
                     /locus_tag="IV236_000761"
     CDS             5628..6584
                     /locus_tag="IV236_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8770980.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6628..7140
                     /locus_tag="IV236_000762"
     CDS             6628..7140
                     /locus_tag="IV236_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8770981.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7396..7944)
                     /locus_tag="IV236_000763"
     CDS             complement(7396..7944)
                     /locus_tag="IV236_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8770982.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8033..9214)
                     /locus_tag="IV236_000764"
     CDS             complement(8033..9214)
                     /locus_tag="IV236_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8770983.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9450..10958)
                     /locus_tag="IV236_000765"
     CDS             complement(9450..10958)
                     /locus_tag="IV236_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8770984.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11117..11308
                     /locus_tag="IV236_000766"
     CDS             11117..11308
                     /locus_tag="IV236_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770985.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11511..12428
                     /locus_tag="IV236_000767"
     CDS             11511..12428
                     /locus_tag="IV236_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8770986.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12421..13494
                     /locus_tag="IV236_000768"
     CDS             12421..13494
                     /locus_tag="IV236_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770987.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13642..14160
                     /locus_tag="IV236_000769"
     CDS             13642..14160
                     /locus_tag="IV236_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770988.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14141..14533
                     /locus_tag="IV236_000770"
     CDS             14141..14533
                     /locus_tag="IV236_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8770989.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14609..15544
                     /locus_tag="IV236_000771"
     CDS             14609..15544
                     /locus_tag="IV236_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8770990.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15677..16456
                     /locus_tag="IV236_000772"
     CDS             15677..16456
                     /locus_tag="IV236_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8770991.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16449..16766
                     /locus_tag="IV236_000773"
     CDS             16449..16766
                     /locus_tag="IV236_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8770992.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17010..18620
                     /locus_tag="IV236_000774"
     CDS             17010..18620
                     /locus_tag="IV236_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8770993.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18777..19625
                     /locus_tag="IV236_000775"
     CDS             18777..19625
                     /locus_tag="IV236_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8770994.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19856..20335
                     /locus_tag="IV236_000776"
     CDS             19856..20335
                     /locus_tag="IV236_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770995.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20368..22596
                     /locus_tag="IV236_000777"
     CDS             20368..22596
                     /locus_tag="IV236_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8770996.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22725..24368
                     /locus_tag="IV236_000778"
     CDS             22725..24368
                     /locus_tag="IV236_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8770997.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
BASE COUNT         8048 a         4342 c         4951 g         7042 t
ORIGIN      
        1 actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat
       61 ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata
      121 cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca
      181 catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag
      241 tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta
      301 gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc
      361 aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag
      421 aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg
      481 ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta
      541 cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa
      601 aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa
      661 ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg
      721 atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt
      781 tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac
      841 ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac
      901 gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat
      961 caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta
     1021 tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt
     1081 ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga
     1141 attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc
     1201 cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt
     1261 gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc
     1321 attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca
     1381 agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa
     1441 gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat
     1501 cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc
     1561 aagcatgtta cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat
     1621 cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa
     1681 aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt
     1741 ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg
     1801 aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta
     1861 gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa
     1921 aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt
     1981 aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt
     2041 cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta
     2101 tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt
     2161 ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca
     2221 gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt
     2281 ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc
     2341 ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga
     2401 gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc
     2461 ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag aagtatccat
     2521 tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg
     2581 ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta
     2641 cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt
     2701 ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa
     2761 acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa
     2821 cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac
     2881 atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa
     2941 tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg
     3001 caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt
     3061 ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg
     3121 gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg
     3181 cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc
     3241 tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa
     3301 ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt
     3361 taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa
     3421 aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg
     3481 atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa
     3541 atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa
     3601 gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct
     3661 ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt
     3721 caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta
     3781 gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga
     3841 atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt
     3901 atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg
     3961 tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa
     4021 agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg
     4081 aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga
     4141 gtgagtgatg tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt
     4201 actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct
     4261 ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat
     4321 tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata
     4381 ttttgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac
     4441 cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca
     4501 gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt
     4561 gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa
     4621 aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat
     4681 aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga tttattgggt
     4741 catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc
     4801 aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg
     4861 tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg
     4921 gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat
     4981 tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt
     5041 gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag
     5101 ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat
     5161 aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga
     5221 ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca
     5281 tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca
     5341 aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc
     5401 gtaaaatatt ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa
     5461 gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca
     5521 atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa
     5581 caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt
     5641 atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc
     5701 tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg
     5761 tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt gatccaaaat ccccaagtag
     5821 gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg
     5881 tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat tcacctccag
     5941 cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg
     6001 caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg
     6061 aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag
     6121 ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg
     6181 gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag
     6241 cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg
     6301 tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata
     6361 aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg
     6421 aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt
     6481 cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta
     6541 aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg
     6601 aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta
     6661 gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct
     6721 caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa
     6781 gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc
     6841 gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta
     6901 tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc
     6961 ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact
     7021 tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg
     7081 atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga
     7141 ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg
     7201 atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa
     7261 atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac
     7321 tcccttcatc gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag
     7381 tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa
     7441 cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc
     7501 taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt
     7561 tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt
     7621 cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc
     7681 tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg
     7741 gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa
     7801 ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat
     7861 cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc
     7921 gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa
     7981 gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta tattatacca
     8041 gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca
     8101 atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa
     8161 tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg
     8221 tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg
     8281 taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc
     8341 tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata
     8401 tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag
     8461 caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg
     8521 tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat
     8581 ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat
     8641 atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag
     8701 ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg
     8761 aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt
     8821 tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa
     8881 tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg
     8941 aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt
     9001 gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac
     9061 caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta
     9121 gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga
     9181 caaataaacc aataatacct aataaataac tcaaataatc actcctattc caaattccgt
     9241 tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt
     9301 tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa
     9361 aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt
     9421 cgttttcaac atttataagt aaactttttc tattggtaat atagattttc ggaagggtag
     9481 acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg
     9541 accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact
     9601 tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt
     9661 agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact
     9721 aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag
     9781 ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct
     9841 ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta
     9901 tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct
     9961 tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg
    10021 cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta
    10081 tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca
    10141 gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc
    10201 cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg
    10261 acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga
    10321 acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt
    10381 tcatgataca gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc
    10441 actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag
    10501 acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg
    10561 tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg
    10621 ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata
    10681 tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca
    10741 tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca
    10801 tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca
    10861 aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct
    10921 tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta
    10981 aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa
    11041 caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg
    11101 ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat
    11161 ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac
    11221 gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt
    11281 cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct
    11341 gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag
    11401 cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact
    11461 attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc
    11521 cagatacaaa tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata
    11581 caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc
    11641 aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga
    11701 tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc
    11761 acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt
    11821 ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt
    11881 accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta
    11941 agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa
    12001 tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac
    12061 aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc
    12121 gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg
    12181 aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag
    12241 cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa
    12301 ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct
    12361 tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt
    12421 atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa
    12481 gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc
    12541 cctatgctag aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat
    12601 actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa
    12661 gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat
    12721 ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat
    12781 cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac
    12841 ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct
    12901 tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa
    12961 ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt
    13021 ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct
    13081 ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat
    13141 cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca
    13201 ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat
    13261 gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag
    13321 atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca
    13381 aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt
    13441 gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat
    13501 aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc
    13561 cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat
    13621 gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct
    13681 gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact
    13741 gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt
    13801 ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg
    13861 atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa
    13921 aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt
    13981 atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa
    14041 ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg
    14101 ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag
    14161 tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca
    14221 atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca
    14281 agaaaatggt caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact
    14341 gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga
    14401 agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag
    14461 tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt
    14521 gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga
    14581 gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc
    14641 cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc
    14701 aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa
    14761 tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca
    14821 atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt
    14881 cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga
    14941 agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag
    15001 ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat
    15061 gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg
    15121 tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat
    15181 tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat
    15241 tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt
    15301 atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac
    15361 agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa
    15421 agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc
    15481 tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa
    15541 ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat
    15601 aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat
    15661 taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc
    15721 agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca
    15781 aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg
    15841 cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt
    15901 gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat
    15961 gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta
    16021 tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata
    16081 tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg
    16141 ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt
    16201 catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt
    16261 attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt
    16321 cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa
    16381 actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc
    16441 caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat
    16501 atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca
    16561 gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt
    16621 tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag
    16681 tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt
    16741 ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac
    16801 ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag
    16861 tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt
    16921 aggaattaga gagggatttg tccttctatg attttttgct ataataggga agatgtttat
    16981 tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt
    17041 ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc
    17101 taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct
    17161 atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa
    17221 aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa
    17281 gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg
    17341 ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca
    17401 gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc
    17461 aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag
    17521 tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga accatattag
    17581 aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata
    17641 tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc
    17701 gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt
    17761 ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac
    17821 cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc
    17881 gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag
    17941 ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa
    18001 aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa
    18061 tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg
    18121 gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta
    18181 cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta
    18241 tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct
    18301 tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag
    18361 aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca
    18421 ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa
    18481 tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag
    18541 tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc
    18601 atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa
    18661 ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta
    18721 cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga
    18781 aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg
    18841 acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg
    18901 atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt
    18961 cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg
    19021 acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg
    19081 cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta
    19141 tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg
    19201 atgaaatact tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag
    19261 atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca
    19321 gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa
    19381 aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata
    19441 acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga
    19501 ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt
    19561 tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc
    19621 aataattcct tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct
    19681 acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc
    19741 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
    19801 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
    19861 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
    19921 tggaagaaat gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa
    19981 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
    20041 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca
    20101 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
    20161 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
    20221 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
    20281 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
    20341 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
    20401 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
    20461 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
    20521 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
    20581 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
    20641 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
    20701 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
    20761 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
    20821 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
    20881 gaaaaaggat atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
    20941 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
    21001 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
    21061 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
    21121 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
    21181 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
    21241 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
    21301 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
    21361 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
    21421 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc
    21481 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
    21541 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
    21601 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
    21661 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
    21721 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
    21781 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
    21841 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
    21901 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
    21961 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
    22021 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
    22081 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
    22141 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
    22201 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
    22261 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
    22321 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
    22381 aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
    22441 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
    22501 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
    22561 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
    22621 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
    22681 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
    22741 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
    22801 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
    22861 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
    22921 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
    22981 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
    23041 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
    23101 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
    23161 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
    23221 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
    23281 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
    23341 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
    23401 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
    23461 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
    23521 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
    23581 aagggcaatt agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg
    23641 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
    23701 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
    23761 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
    23821 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
    23881 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
    23941 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
    24001 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
    24061 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
    24121 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
    24181 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
    24241 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
    24301 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
    24361 acaagtaaaa cttcctcgtt aaa
//
LOCUS       DACDID010000024        23740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDID010000024 DACDID010000000
VERSION     DACDID010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.024"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..1061
                     /gene="mvk"
                     /locus_tag="IV236_000779"
     CDS             117..1061
                     /gene="mvk"
                     /locus_tag="IV236_000779"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8770998.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1054..2031
                     /gene="mvaD"
                     /locus_tag="IV236_000780"
     CDS             1054..2031
                     /gene="mvaD"
                     /locus_tag="IV236_000780"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8770999.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2044..3129
                     /locus_tag="IV236_000781"
     CDS             2044..3129
                     /locus_tag="IV236_000781"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8771000.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3126..4190
                     /locus_tag="IV236_000782"
     CDS             3126..4190
                     /locus_tag="IV236_000782"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8771001.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4439..5242)
                     /locus_tag="IV236_000783"
     CDS             complement(4439..5242)
                     /locus_tag="IV236_000783"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8771002.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5262..6104)
                     /gene="kduI"
                     /locus_tag="IV236_000784"
     CDS             complement(5262..6104)
                     /gene="kduI"
                     /locus_tag="IV236_000784"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8771003.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6120..7127)
                     /locus_tag="IV236_000785"
     CDS             complement(6120..7127)
                     /locus_tag="IV236_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8771004.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7134..7778)
                     /locus_tag="IV236_000786"
     CDS             complement(7134..7778)
                     /locus_tag="IV236_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8771005.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7878..8636)
                     /locus_tag="IV236_000787"
     CDS             complement(7878..8636)
                     /locus_tag="IV236_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8771006.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8763..9914)
                     /gene="fucO"
                     /locus_tag="IV236_000788"
     CDS             complement(8763..9914)
                     /gene="fucO"
                     /locus_tag="IV236_000788"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8771007.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(9996..10307)
                     /gene="rhaM"
                     /locus_tag="IV236_000789"
     CDS             complement(9996..10307)
                     /gene="rhaM"
                     /locus_tag="IV236_000789"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8771008.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10309..11154)
                     /gene="rhaD"
                     /locus_tag="IV236_000790"
     CDS             complement(10309..11154)
                     /gene="rhaD"
                     /locus_tag="IV236_000790"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8771009.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11168..12424)
                     /gene="rhaA"
                     /locus_tag="IV236_000791"
     CDS             complement(11168..12424)
                     /gene="rhaA"
                     /locus_tag="IV236_000791"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8771010.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12421..13881)
                     /gene="rhaB"
                     /locus_tag="IV236_000792"
     CDS             complement(12421..13881)
                     /gene="rhaB"
                     /locus_tag="IV236_000792"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8771011.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14034..14903
                     /locus_tag="IV236_000793"
     CDS             14034..14903
                     /locus_tag="IV236_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8771012.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15029..15928)
                     /locus_tag="IV236_000794"
     CDS             complement(15029..15928)
                     /locus_tag="IV236_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8771013.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16093..16917)
                     /locus_tag="IV236_000795"
     CDS             complement(16093..16917)
                     /locus_tag="IV236_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8771014.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(16928..18223)
                     /locus_tag="IV236_000796"
     CDS             complement(16928..18223)
                     /locus_tag="IV236_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8771015.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18239..18718)
                     /locus_tag="IV236_000797"
     CDS             complement(18239..18718)
                     /locus_tag="IV236_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8771016.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18724..19752)
                     /locus_tag="IV236_000798"
     CDS             complement(18724..19752)
                     /locus_tag="IV236_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8771017.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19769..20875)
                     /locus_tag="IV236_000799"
     CDS             complement(19769..20875)
                     /locus_tag="IV236_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8771018.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21104..23701)
                     /gene="adhE"
                     /locus_tag="IV236_000800"
                     /gene_synonym="adhC"
     CDS             complement(21104..23701)
                     /gene="adhE"
                     /locus_tag="IV236_000800"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8771019.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         6686 a         5279 c         4316 g         7459 t
ORIGIN      
        1 ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt
       61 tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg
      121 caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg
      181 tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg
      241 aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg
      301 aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg
      361 acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg
      421 gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc
      481 ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccacggta
      541 atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag
      601 ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag
      661 gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc
      721 ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag
      781 caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc
      841 tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata
      901 ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag
      961 cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg
     1021 aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat
     1081 accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg
     1141 aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt catcttttct
     1201 gatagctata tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca
     1261 aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct
     1321 tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg
     1381 ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct
     1441 ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc
     1501 gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat
     1561 tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg
     1621 tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta
     1681 gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa
     1741 cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc
     1801 cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa
     1861 ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt
     1921 ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa
     1981 gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt
     2041 tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt
     2101 gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa
     2161 tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg
     2221 acaagacgca acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt
     2281 gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat
     2341 gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct
     2401 agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta
     2461 tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt
     2521 tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac
     2581 catccttggc tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat
     2641 tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt attgattggt
     2701 tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa
     2761 gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg
     2821 atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga
     2881 caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac
     2941 aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg
     3001 ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca
     3061 tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa
     3121 aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa
     3181 aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg
     3241 atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg
     3301 cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa
     3361 gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt
     3421 tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca
     3481 tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg
     3541 ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt
     3601 ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg
     3661 taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag
     3721 ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc
     3781 gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat
     3841 tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca
     3901 atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa
     3961 ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat
     4021 ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac
     4081 accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg
     4141 acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt
     4201 tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag
     4261 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
     4321 aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg
     4381 tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt
     4441 aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa
     4501 cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc
     4561 cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt
     4621 tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc
     4681 cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta
     4741 atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg
     4801 agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg
     4861 aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca
     4921 tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac
     4981 ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc
     5041 agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg
     5101 attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa
     5161 tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa
     5221 acatatccat actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt
     5281 tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag
     5341 taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca
     5401 ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta
     5461 tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa
     5521 ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag
     5581 ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct
     5641 tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga
     5701 tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat
     5761 acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt
     5821 tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc
     5881 tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta
     5941 ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct
     6001 ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg
     6061 atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc
     6121 tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat
     6181 tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg
     6241 atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct
     6301 ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc cacatattca
     6361 tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg
     6421 gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt
     6481 agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt
     6541 tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct
     6601 tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt
     6661 tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg
     6721 attcctgaaa catgaaatat atctattcct tggaataact cctctagcga ccattccaaa
     6781 tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg
     6841 ccactaccag tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc
     6901 gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt
     6961 acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct
     7021 tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct
     7081 gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc
     7141 ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct
     7201 cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg
     7261 aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc
     7321 atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg
     7381 acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt
     7441 aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta
     7501 acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg
     7561 cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact
     7621 tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt
     7681 gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg
     7741 gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt
     7801 agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat
     7861 tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg
     7921 agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca
     7981 ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca
     8041 cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt
     8101 ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca
     8161 agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa
     8221 ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga
     8281 tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca
     8341 tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt
     8401 tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa
     8461 tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag
     8521 gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag
     8581 tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa
     8641 tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat
     8701 atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt
     8761 ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt
     8821 cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt
     8881 acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca
     8941 ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga
     9001 acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt
     9061 ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga
     9121 gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt
     9181 cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg
     9241 ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg
     9301 ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc
     9361 ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca
     9421 acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct
     9481 gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca
     9541 gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg
     9601 gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc
     9661 tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg
     9721 tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata
     9781 ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga
     9841 aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc
     9901 attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa
     9961 gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat
    10021 tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac
    10081 catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact
    10141 aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg
    10201 tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct
    10261 tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc
    10321 tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa
    10381 atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt
    10441 atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt
    10501 tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc
    10561 agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc
    10621 ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg
    10681 ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac
    10741 gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa
    10801 ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc
    10861 ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata
    10921 tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat
    10981 cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag
    11041 taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt
    11101 cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc
    11161 cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc
    11221 aatccaaacc aactggaact tgatttttgt aacagaaata attccataca tcggcatacg
    11281 ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca
    11341 tttctttcaa tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc
    11401 tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga
    11461 tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat
    11521 catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga
    11581 ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa
    11641 aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt
    11701 catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat
    11761 cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac
    11821 gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt
    11881 tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt
    11941 tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt
    12001 ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt
    12061 ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt
    12121 cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat
    12181 aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc
    12241 ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat
    12301 ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg
    12361 tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt
    12421 tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc
    12481 atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat
    12541 attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc
    12601 actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg
    12661 attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc
    12721 tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat
    12781 agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc
    12841 tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg
    12901 tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg
    12961 ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg
    13021 ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt
    13081 tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg
    13141 acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac
    13201 atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg
    13261 ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc
    13321 ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac
    13381 catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt
    13441 tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg
    13501 aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc
    13561 tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc
    13621 aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt
    13681 ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg
    13741 atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa
    13801 agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat
    13861 ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat tagcacttta
    13921 ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt atattttgct
    13981 tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt
    14041 actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta
    14101 cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa
    14161 tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg
    14221 gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt
    14281 ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg
    14341 attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg
    14401 gccataacac cttccattca acaggagatc catcagaaaa caagatccac catttgatca
    14461 cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag
    14521 ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag
    14581 aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa
    14641 cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag
    14701 atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt
    14761 acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag
    14821 ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga
    14881 gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc aaaatatttt
    14941 gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa caacagcagc
    15001 tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt tcataatgaa
    15061 ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg aactctctct
    15121 tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat acagcactag
    15181 agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc attacatact
    15241 gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat ttgctgacaa
    15301 aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt gagatatatt
    15361 gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc gcactctctt
    15421 ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt tcatatttta
    15481 tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat ttttgcttca
    15541 atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt tgaaaacaag
    15601 ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg acaaagatat
    15661 agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca ttggaatgga
    15721 tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt
    15781 ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc gttttatttt
    15841 ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc gtattgactt
    15901 gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca cttatgcaca
    15961 attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac tcttataaaa
    16021 aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta gttaaccaac
    16081 gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat cttttgtttc
    16141 ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga tttttgtgta
    16201 ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga cacgaaaatc
    16261 tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt ctaccaattg
    16321 gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa tgattcctag
    16381 atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat acaccggatg
    16441 attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt ttaccaagcg
    16501 acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt ccgtatattg
    16561 gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa aggaagaagc
    16621 atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta aatctggatg
    16681 aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt ctaaaactcg
    16741 tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta tttcaggaaa
    16801 cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt tttctgcaag
    16861 atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt tcaacataac
    16921 tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag taagtaatca
    16981 aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc attgcctctg
    17041 cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc ggcgtgatgg
    17101 agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca acccctacac
    17161 ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc acatccataa
    17221 acatacccac cagtaataac acaagattga ctaataacag aataataaat ttattatccg
    17281 ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag
    17341 acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct actgattgaa
    17401 acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata gcaatcaaca
    17461 gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc gtcaagatac
    17521 caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt atttttccag
    17581 catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca acgatgaatg
    17641 ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc ataaacaaag
    17701 cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta cttggaggaa
    17761 tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc
    17821 cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg gcaatggccg
    17881 agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc aaagcccccg
    17941 gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc cctccattat
    18001 tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac
    18061 ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata actaacaata
    18121 aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat acaagcaaca
    18181 caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc ctccattttt
    18241 atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg atcaggctat
    18301 agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc ataggaatac
    18361 ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg ccatacacca
    18421 tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa attttacttt
    18481 ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa
    18541 catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga
    18601 ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca acagcgacca
    18661 tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc attttcatca
    18721 cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag gatcgttttt
    18781 gaatatatca tgaagtgggg caactgcctc tcgaaacggt gtattatcta catcattgaa
    18841 ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc
    18901 ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt cctgctcatc
    18961 agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac gtgtatgctc
    19021 atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga caaattcatt
    19081 gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat cacttcccat
    19141 aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc tttgcatcac
    19201 acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta ccatataaaa
    19261 gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt tggtcgattg
    19321 gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt cttcatcatt
    19381 gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca acgctgaggc
    19441 acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc
    19501 acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt tttcttttac
    19561 taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg cataagcaaa
    19621 gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac tagaattatt
    19681 tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa acagcagtga
    19741 tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat tctgtcaaac
    19801 aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc cgataaaaat
    19861 ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct ttttcatcga
    19921 tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct tgtggtaaat
    19981 aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca gaagattcta
    20041 cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac tgataagttt
    20101 ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt
    20161 tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag cagtttccac
    20221 gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg tgataccaca
    20281 atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa cgagcttctt
    20341 ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc actgtcatca
    20401 tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca tgctgtaacc
    20461 cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt tctaaataag
    20521 gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat agcggtgcca
    20581 ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac cattcattga
    20641 ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata ccatatagac
    20701 cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc agtccatcaa
    20761 agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca acgagcaggt
    20821 caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata atcatcttta
    20881 cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata ccgtccacta
    20941 taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa ataacaaaag
    21001 taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt ttccaagttc
    21061 tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg ttgtaagcat
    21121 caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct gtcgtacatt
    21181 ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt tcttgtgtga
    21241 ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca taaacagccg
    21301 ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag aattttgcga
    21361 tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc gcatgttttt
    21421 gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt gtccgtccat
    21481 gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata cctaggaatg
    21541 cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca cgtgattcca
    21601 tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt gcttgtaggc
    21661 tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt gcatgtgtta
    21721 acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg aattgaggat
    21781 cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc acatgtgttt
    21841 cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat gttgtcggga
    21901 tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta cggatatcca
    21961 agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac atccacattc
    22021 ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct ggcttgaagt
    22081 ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt tcaacatctg
    22141 agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga actgtatcgc
    22201 agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca acgttttcca
    22261 ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt aatttgaacc
    22321 attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt acagcagaga
    22381 cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat gacggaatca
    22441 tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact aaaatacgac
    22501 aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa tggataactg
    22561 ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac gcatggtccg
    22621 tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat agcggataat
    22681 ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt acactgattc
    22741 ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg accgcatatt
    22801 ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt acgatataaa
    22861 cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc acgatcacag
    22921 cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc aaatcgttca
    22981 ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct ggacctacac
    23041 ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca cctgtcgcta
    23101 agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc gaaggatgtt
    23161 cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca cgtaccactc
    23221 gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg attggatttc
    23281 ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc gttgttggag
    23341 ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt tggacatctt
    23401 cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat tcggacgcat
    23461 acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct acagccatct
    23521 tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg atatggtcta
    23581 ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt tctgccaaat
    23641 catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt actttagcca
    23701 tcattatcct cctaatgggt catggttatt tgttaaataa
//
LOCUS       DACDID010000025        23583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDID010000025 DACDID010000000
VERSION     DACDID010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.025"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..1375
                     /locus_tag="IV236_000801"
     CDS             35..1375
                     /locus_tag="IV236_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8771020.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1593..2036
                     /locus_tag="IV236_000802"
     CDS             1593..2036
                     /locus_tag="IV236_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8771021.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="IV236_000803"
     CDS             complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="IV236_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8771022.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4361..5584)
                     /locus_tag="IV236_000804"
     CDS             complement(4361..5584)
                     /locus_tag="IV236_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8771023.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5615..6454)
                     /locus_tag="IV236_000805"
     CDS             complement(5615..6454)
                     /locus_tag="IV236_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8771024.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6471..7151)
                     /locus_tag="IV236_000806"
     CDS             complement(6471..7151)
                     /locus_tag="IV236_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771025.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7153..8226)
                     /locus_tag="IV236_000807"
     CDS             complement(7153..8226)
                     /locus_tag="IV236_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8771026.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8873..9544)
                     /locus_tag="IV236_000808"
     CDS             complement(8873..9544)
                     /locus_tag="IV236_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8771027.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="IV236_000809"
     CDS             complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="IV236_000809"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8771028.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12968..13405
                     /gene="ndk"
                     /locus_tag="IV236_000810"
     CDS             12968..13405
                     /gene="ndk"
                     /locus_tag="IV236_000810"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8771029.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13427..14293)
                     /locus_tag="IV236_000811"
     CDS             complement(13427..14293)
                     /locus_tag="IV236_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8771030.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14453..16546)
                     /locus_tag="IV236_000812"
     CDS             complement(14453..16546)
                     /locus_tag="IV236_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8771031.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17295..17918)
                     /locus_tag="IV236_000813"
     CDS             complement(17295..17918)
                     /locus_tag="IV236_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771032.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18077..19474)
                     /locus_tag="IV236_000814"
     CDS             complement(18077..19474)
                     /locus_tag="IV236_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8771033.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19506..20477)
                     /locus_tag="IV236_000815"
     CDS             complement(19506..20477)
                     /locus_tag="IV236_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8771034.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20851..21276
                     /locus_tag="IV236_000816"
     CDS             20851..21276
                     /locus_tag="IV236_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771035.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21764..22465)
                     /locus_tag="IV236_000817"
     CDS             complement(21764..22465)
                     /locus_tag="IV236_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8771036.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22481..22921)
                     /locus_tag="IV236_000818"
     CDS             complement(22481..22921)
                     /locus_tag="IV236_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8771037.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22921..23496)
                     /locus_tag="IV236_000819"
     CDS             complement(22921..23496)
                     /locus_tag="IV236_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8771038.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6779 a         4895 c         4059 g         7850 t
ORIGIN      
        1 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
       61 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
      121 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
      181 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
      241 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
      301 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
      361 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
      421 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
      481 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
      541 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
      601 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
      661 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
      721 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
      781 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
      841 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
      901 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
      961 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
     1021 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
     1081 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
     1141 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
     1201 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
     1261 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
     1321 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
     1381 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
     1441 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
     1501 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
     1561 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
     1621 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
     1681 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
     1741 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
     1801 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
     1861 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
     1921 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
     1981 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
     2041 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
     2101 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
     2161 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
     2221 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
     2281 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
     2341 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
     2401 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
     2461 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
     2521 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
     2581 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
     2641 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
     2701 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
     2761 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
     2821 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
     2881 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
     2941 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
     3001 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
     3061 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
     3121 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
     3181 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
     3241 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
     3301 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
     3361 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
     3421 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
     3481 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
     3541 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
     3601 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
     3661 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
     3721 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
     3781 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
     3841 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
     3901 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
     3961 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
     4021 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
     4081 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
     4141 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
     4201 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
     4261 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
     4321 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
     4381 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
     4441 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
     4501 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
     4561 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
     4621 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
     4681 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
     4741 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
     4801 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
     4861 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
     4921 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
     4981 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
     5041 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
     5101 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
     5161 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
     5221 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
     5281 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
     5341 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
     5401 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
     5461 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
     5521 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
     5581 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
     5641 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
     5701 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
     5761 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
     5821 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
     5881 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
     5941 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
     6001 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
     6061 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
     6121 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
     6181 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
     6241 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
     6301 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
     6361 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
     6421 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
     6481 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
     6541 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
     6601 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
     6661 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
     6721 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
     6781 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
     6841 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
     6901 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
     6961 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
     7021 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tattgtttgc
     7081 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
     7141 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
     7201 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
     7261 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
     7321 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
     7381 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
     7441 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
     7501 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
     7561 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
     7621 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
     7681 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
     7741 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
     7801 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
     7861 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
     7921 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
     7981 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
     8041 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
     8101 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
     8161 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
     8221 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
     8281 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
     8341 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
     8401 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
     8461 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
     8521 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
     8581 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
     8641 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
     8701 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
     8761 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
     8821 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
     8881 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
     8941 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
     9001 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
     9061 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
     9121 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
     9181 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
     9241 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
     9301 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
     9361 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
     9421 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
     9481 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
     9541 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
     9601 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
     9661 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
     9721 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag
     9781 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
     9841 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
     9901 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
     9961 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    10021 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    10081 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    10141 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    10201 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    10261 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    10321 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    10381 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    10441 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    10501 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    10561 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    10621 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    10681 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    10741 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    10801 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    10861 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    10921 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    10981 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    11041 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    11101 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    11161 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    11221 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    11281 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    11341 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    11401 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    11461 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    11521 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    11581 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    11641 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    11701 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    11761 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    11821 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    11881 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    11941 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    12001 atctccagga gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa
    12061 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    12121 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    12181 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    12241 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    12301 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    12361 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    12421 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    12481 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    12541 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    12601 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    12661 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    12721 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    12781 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    12841 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    12901 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    12961 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    13021 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    13081 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    13141 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    13201 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    13261 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    13321 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    13381 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    13441 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    13501 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    13561 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    13621 gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    13681 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    13741 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    13801 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    13861 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    13921 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    13981 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    14041 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    14101 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    14161 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    14221 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    14281 caccatcata cataatagtt ctccttttta atttaaattt ttccacattc aataaataga
    14341 tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac
    14401 caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat
    14461 tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat
    14521 taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat
    14581 tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg
    14641 gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct
    14701 tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga
    14761 tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa
    14821 agttcccatt tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa
    14881 aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga
    14941 tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca
    15001 atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa
    15061 gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca
    15121 caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg
    15181 attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca
    15241 aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa
    15301 gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata
    15361 aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat
    15421 caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta
    15481 cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg
    15541 cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg
    15601 tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga
    15661 aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt
    15721 cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga
    15781 tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct
    15841 ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag
    15901 tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa
    15961 tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta
    16021 gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga
    16081 cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca
    16141 cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg
    16201 acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga
    16261 attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca
    16321 aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata
    16381 aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta
    16441 atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca
    16501 aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc
    16561 tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt
    16621 atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac
    16681 cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac
    16741 tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa
    16801 ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa
    16861 acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc
    16921 tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag acctcttgat
    16981 acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct aattttacaa
    17041 cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    17101 aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac ggcaggctca
    17161 agacctcttg atacacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca
    17221 aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
    17281 acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta
    17341 ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa
    17401 ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca
    17461 tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa
    17521 taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa
    17581 agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt
    17641 acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat
    17701 tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt tgagaattct
    17761 tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca
    17821 agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg
    17881 aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa
    17941 ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat
    18001 cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc
    18061 tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat
    18121 gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat
    18181 atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc
    18241 ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa
    18301 attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt
    18361 ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat
    18421 tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt
    18481 tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat
    18541 cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat
    18601 ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa
    18661 gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga
    18721 atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt
    18781 cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca
    18841 tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga
    18901 tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga
    18961 ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa
    19021 aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt
    19081 tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt
    19141 tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc
    19201 cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag
    19261 tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag
    19321 gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct
    19381 ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc
    19441 accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt
    19501 tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc
    19561 tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt
    19621 ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag
    19681 tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca
    19741 ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct
    19801 tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc
    19861 gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct
    19921 gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc
    19981 acttttgcta cactttctcg atcattcact aatattgtat caatacctgc tggtccttct
    20041 ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac
    20101 tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt
    20161 ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt
    20221 tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg
    20281 tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt
    20341 tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt
    20401 tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca
    20461 tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt
    20521 tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa
    20581 ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac aagtatttga
    20641 accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt
    20701 tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt
    20761 gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat
    20821 ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg
    20881 tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg
    20941 gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta
    21001 tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat
    21061 aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct
    21121 gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga atatattatt
    21181 tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt
    21241 ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg
    21301 tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg
    21361 gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt
    21421 taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt
    21481 taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa
    21541 tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt cttcctttga
    21601 tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag
    21661 atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac
    21721 atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac
    21781 cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta
    21841 atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga
    21901 atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac cattgattat
    21961 agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat
    22021 agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga
    22081 ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta
    22141 ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat
    22201 ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag
    22261 catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact
    22321 gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg
    22381 gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg
    22441 ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt
    22501 aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat
    22561 acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga
    22621 tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg
    22681 tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat
    22741 ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt
    22801 gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac
    22861 gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca
    22921 ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta attcattgac
    22981 cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc
    23041 ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa
    23101 tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa
    23161 ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa
    23221 taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg
    23281 caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag
    23341 tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat
    23401 ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt
    23461 accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa
    23521 gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta
    23581 gtg
//
LOCUS       DACDID010000026        23477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDID010000026 DACDID010000000
VERSION     DACDID010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.026"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IV236_000820"
     CDS             173..1309
                     /locus_tag="IV236_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8771039.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IV236_000821"
     CDS             1446..1790
                     /locus_tag="IV236_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8771040.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IV236_000822"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IV236_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8771041.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IV236_000823"
     CDS             complement(3485..4084)
                     /locus_tag="IV236_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8771042.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IV236_000824"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IV236_000824"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8771043.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IV236_000825"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IV236_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8771044.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IV236_000826"
     CDS             7290..8408
                     /locus_tag="IV236_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8771045.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IV236_000827"
     CDS             complement(8468..9760)
                     /locus_tag="IV236_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8771046.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IV236_000828"
     CDS             9910..10470
                     /locus_tag="IV236_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8771047.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IV236_000829"
     CDS             10599..10976
                     /locus_tag="IV236_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8771048.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IV236_000830"
     CDS             10909..12981
                     /locus_tag="IV236_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8771049.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IV236_000831"
     CDS             complement(13192..14139)
                     /locus_tag="IV236_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8771050.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="IV236_000832"
     CDS             14385..14849
                     /locus_tag="IV236_000832"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8771051.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="IV236_000833"
     CDS             complement(14911..15954)
                     /locus_tag="IV236_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8771052.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="IV236_000834"
     CDS             complement(16331..17296)
                     /locus_tag="IV236_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8771053.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="IV236_000835"
     CDS             17530..18375
                     /locus_tag="IV236_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8771054.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="IV236_000836"
     CDS             18566..19753
                     /locus_tag="IV236_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8771055.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="IV236_000837"
     CDS             19759..20115
                     /locus_tag="IV236_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8771056.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="IV236_000838"
     CDS             20117..20455
                     /locus_tag="IV236_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8771057.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20850..21884
                     /locus_tag="IV236_000839"
     CDS             20850..21884
                     /locus_tag="IV236_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8771058.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="IV236_000840"
     CDS             21877..22563
                     /locus_tag="IV236_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771059.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="IV236_000841"
     CDS             22587..23435
                     /locus_tag="IV236_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8771060.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         7704 a         4254 c         5002 g         6517 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggctt
//
LOCUS       DACDID010000027        23261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDID010000027 DACDID010000000
VERSION     DACDID010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.027"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV236_000842"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV236_000842"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8771061.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IV236_000843"
     CDS             complement(1172..1867)
                     /locus_tag="IV236_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8771062.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IV236_000844"
     CDS             complement(1880..2596)
                     /locus_tag="IV236_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8771063.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IV236_000845"
     CDS             2926..3534
                     /locus_tag="IV236_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8771064.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IV236_000846"
     CDS             3859..4182
                     /locus_tag="IV236_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8771065.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IV236_000847"
     CDS             4364..5734
                     /locus_tag="IV236_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8771066.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IV236_000848"
     CDS             complement(5864..7165)
                     /locus_tag="IV236_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8771067.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IV236_000849"
     CDS             complement(7519..9510)
                     /locus_tag="IV236_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8771068.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFSNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IV236_000850"
     CDS             complement(9654..9920)
                     /locus_tag="IV236_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8771069.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV236_000851"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV236_000851"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8771070.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV236_000852"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV236_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8771071.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IV236_000853"
     CDS             13264..13827
                     /locus_tag="IV236_000853"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8771072.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IV236_000854"
     CDS             complement(14080..14601)
                     /locus_tag="IV236_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8771073.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IV236_000855"
     CDS             complement(14604..15446)
                     /locus_tag="IV236_000855"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8771074.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IV236_000856"
     CDS             complement(15439..16164)
                     /locus_tag="IV236_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8771075.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IV236_000857"
     CDS             16340..16636
                     /locus_tag="IV236_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771076.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IV236_000858"
     CDS             17204..18796
                     /locus_tag="IV236_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8771077.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IV236_000859"
     CDS             18809..19549
                     /locus_tag="IV236_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8771078.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IV236_000860"
     CDS             19717..21066
                     /locus_tag="IV236_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8771079.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IV236_000861"
     CDS             complement(21194..21784)
                     /locus_tag="IV236_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8771080.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="IV236_000862"
     CDS             22020..23099
                     /locus_tag="IV236_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8771081.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4819 c         4527 g         7254 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattact gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DACDID010000028        23012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDID010000028 DACDID010000000
VERSION     DACDID010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.028"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IV236_000863"
     tRNA            2..74
                     /locus_tag="IV236_000863"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IV236_000864"
     tRNA            78..150
                     /locus_tag="IV236_000864"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IV236_000865"
     tRNA            155..225
                     /locus_tag="IV236_000865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IV236_000866"
     tRNA            238..311
                     /locus_tag="IV236_000866"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IV236_000867"
     tRNA            323..394
                     /locus_tag="IV236_000867"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IV236_000868"
     CDS             660..1853
                     /locus_tag="IV236_000868"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8771082.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IV236_000869"
     CDS             1983..3455
                     /locus_tag="IV236_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771083.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IV236_000870"
     CDS             3538..4326
                     /locus_tag="IV236_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8771084.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IV236_000871"
     CDS             complement(4393..5061)
                     /locus_tag="IV236_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8771085.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IV236_000872"
     CDS             5176..6888
                     /locus_tag="IV236_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771086.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IV236_000873"
     CDS             6891..8660
                     /locus_tag="IV236_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771087.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IV236_000874"
     CDS             complement(8865..9431)
                     /locus_tag="IV236_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8771088.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IV236_000875"
     CDS             complement(9431..10387)
                     /locus_tag="IV236_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8771089.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IV236_000876"
     CDS             complement(10387..11043)
                     /locus_tag="IV236_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8771090.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="IV236_000877"
     CDS             11413..13431
                     /locus_tag="IV236_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8771091.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13847..16261
                     /locus_tag="IV236_000878"
     CDS             13847..16261
                     /locus_tag="IV236_000878"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8771092.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16609..18162
                     /locus_tag="IV236_000879"
     CDS             16609..18162
                     /locus_tag="IV236_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8771093.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18543..20327)
                     /locus_tag="IV236_000880"
     CDS             complement(18543..20327)
                     /locus_tag="IV236_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771094.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20342..22063)
                     /locus_tag="IV236_000881"
     CDS             complement(20342..22063)
                     /locus_tag="IV236_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771095.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22163..22924
                     /locus_tag="IV236_000882"
     CDS             22163..22924
                     /locus_tag="IV236_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8771096.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7253 a         4165 c         4793 g         6801 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttgtgaag
    13741 tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat
    13801 atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca
    13861 taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca
    13921 tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca
    13981 agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa
    14041 aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc
    14101 tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga
    14161 aacattccga cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa
    14221 tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa
    14281 aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat
    14341 ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa
    14401 accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct
    14461 agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc
    14521 agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac
    14581 gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct
    14641 tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac
    14701 agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac
    14761 tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta
    14821 tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga
    14881 ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt
    14941 tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat
    15001 gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa
    15061 aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga
    15121 accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct
    15181 accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt
    15241 agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg
    15301 cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga
    15361 ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga
    15421 catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca
    15481 taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa
    15541 tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt
    15601 caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt
    15661 catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa
    15721 attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat
    15781 cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac
    15841 agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa
    15901 tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact
    15961 cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg
    16021 tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga
    16081 agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg
    16141 aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg
    16201 caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta
    16261 attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga
    16321 acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg
    16381 ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag
    16441 agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact
    16501 acctgaaatt gagagtacat accaaattca taaatttttt attagtggga ctagtgattg
    16561 tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa
    16621 ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
    16681 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
    16741 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
    16801 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
    16861 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
    16921 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
    16981 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
    17041 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
    17101 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
    17161 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
    17221 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
    17281 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
    17341 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
    17401 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
    17461 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
    17521 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
    17581 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
    17641 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
    17701 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
    17761 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
    17821 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
    17881 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
    17941 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
    18001 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
    18061 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
    18121 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
    18181 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
    18241 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
    18301 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
    18361 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
    18421 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
    18481 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
    18541 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
    18601 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
    18661 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
    18721 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
    18781 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
    18841 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
    18901 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
    18961 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
    19021 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
    19081 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
    19141 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
    19201 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
    19261 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
    19321 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
    19381 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
    19441 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
    19501 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat
    19561 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
    19621 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
    19681 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
    19741 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
    19801 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
    19861 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
    19921 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
    19981 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
    20041 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
    20101 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
    20161 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
    20221 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
    20281 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
    20341 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
    20401 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
    20461 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
    20521 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
    20581 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
    20641 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
    20701 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
    20761 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
    20821 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
    20881 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
    20941 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
    21001 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
    21061 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
    21121 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
    21181 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
    21241 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
    21301 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    21361 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    21421 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    21481 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    21541 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    21601 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    21661 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    21721 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    21781 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    21841 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    21901 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    21961 attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag
    22021 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    22081 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    22141 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    22201 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    22261 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    22321 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    22381 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    22441 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
    22501 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    22561 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    22621 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    22681 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    22741 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    22801 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    22861 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    22921 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    22981 ttttctggtt cgattacggg tttaatgtgg gg
//
LOCUS       DACDID010000029        22848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDID010000029 DACDID010000000
VERSION     DACDID010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.029"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..280
                     /gene="nrdH"
                     /locus_tag="IV236_000883"
     CDS             56..280
                     /gene="nrdH"
                     /locus_tag="IV236_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8771097.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            439..2598
                     /gene="nrdE"
                     /locus_tag="IV236_000884"
     CDS             439..2598
                     /gene="nrdE"
                     /locus_tag="IV236_000884"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8771098.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2648..3613
                     /gene="nrdF"
                     /locus_tag="IV236_000885"
     CDS             2648..3613
                     /gene="nrdF"
                     /locus_tag="IV236_000885"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8771099.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3702..4841)
                     /locus_tag="IV236_000886"
     CDS             complement(3702..4841)
                     /locus_tag="IV236_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8771100.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5150..5863)
                     /locus_tag="IV236_000887"
     CDS             complement(5150..5863)
                     /locus_tag="IV236_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8771101.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6117..6818)
                     /gene="nagB"
                     /locus_tag="IV236_000888"
     CDS             complement(6117..6818)
                     /gene="nagB"
                     /locus_tag="IV236_000888"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8771102.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7052..8584
                     /locus_tag="IV236_000889"
     CDS             7052..8584
                     /locus_tag="IV236_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8771103.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8775..9977)
                     /locus_tag="IV236_000890"
     CDS             complement(8775..9977)
                     /locus_tag="IV236_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771104.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10059..10748)
                     /locus_tag="IV236_000891"
     CDS             complement(10059..10748)
                     /locus_tag="IV236_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8771105.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10854..11222)
                     /gene="rplL"
                     /locus_tag="IV236_000892"
     CDS             complement(10854..11222)
                     /gene="rplL"
                     /locus_tag="IV236_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8771106.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11275..11778)
                     /locus_tag="IV236_000893"
     CDS             complement(11275..11778)
                     /locus_tag="IV236_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8771107.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12188..12877)
                     /gene="rplA"
                     /locus_tag="IV236_000894"
     CDS             complement(12188..12877)
                     /gene="rplA"
                     /locus_tag="IV236_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8771108.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12981..13403)
                     /gene="rplK"
                     /locus_tag="IV236_000895"
     CDS             complement(12981..13403)
                     /gene="rplK"
                     /locus_tag="IV236_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8771109.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13746..14414
                     /gene="sdaAB"
                     /locus_tag="IV236_000896"
     CDS             13746..14414
                     /gene="sdaAB"
                     /locus_tag="IV236_000896"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8771110.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14427..15299
                     /gene="sdaAA"
                     /locus_tag="IV236_000897"
     CDS             14427..15299
                     /gene="sdaAA"
                     /locus_tag="IV236_000897"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8771111.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15390..16409)
                     /locus_tag="IV236_000898"
     CDS             complement(15390..16409)
                     /locus_tag="IV236_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8771112.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16582..17469)
                     /locus_tag="IV236_000899"
     CDS             complement(16582..17469)
                     /locus_tag="IV236_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8771113.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17546..18091)
                     /gene="nusG"
                     /locus_tag="IV236_000900"
     CDS             complement(17546..18091)
                     /gene="nusG"
                     /locus_tag="IV236_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8771114.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18192..18362)
                     /gene="secE"
                     /locus_tag="IV236_000901"
     CDS             complement(18192..18362)
                     /gene="secE"
                     /locus_tag="IV236_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8771115.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18386..18538)
                     /gene="rpmG"
                     /locus_tag="IV236_000902"
     CDS             complement(18386..18538)
                     /gene="rpmG"
                     /locus_tag="IV236_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8771116.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18700..19344
                     /locus_tag="IV236_000903"
     CDS             18700..19344
                     /locus_tag="IV236_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8771117.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19400..19903)
                     /locus_tag="IV236_000904"
     CDS             complement(19400..19903)
                     /locus_tag="IV236_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8771118.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19916..19962)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20500..21465
                     /locus_tag="IV236_000905"
     CDS             20500..21465
                     /locus_tag="IV236_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8771119.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21564..22736)
                     /locus_tag="IV236_000906"
     CDS             complement(21564..22736)
                     /locus_tag="IV236_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771120.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22723..>22848)
                     /locus_tag="IV236_000907"
     CDS             complement(22723..>22848)
                     /locus_tag="IV236_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771121.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7142 a         4586 c         4108 g         7012 t
ORIGIN      
        1 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
       61 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
      121 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
      181 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
      241 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
      301 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
      361 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
      421 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
      481 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
      541 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttct atctattatg
      601 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
      661 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
      721 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
      781 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
      841 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
      901 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
      961 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     1021 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     1081 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     1141 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     1201 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     1261 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
     1321 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
     1381 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
     1441 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
     1501 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
     1561 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
     1621 aacggcaaac aggaatatga agtattagga acggatatct catgtaactt aggctcgaca
     1681 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     1741 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     1801 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     1861 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     1921 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     1981 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     2041 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     2101 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     2161 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     2221 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     2281 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
     2341 atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac acgtcgatca aggcatgagt
     2401 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
     2461 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
     2521 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
     2581 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
     2641 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
     2701 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
     2761 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
     2821 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
     2881 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
     2941 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
     3001 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
     3061 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
     3121 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
     3181 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
     3241 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
     3301 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
     3361 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
     3421 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
     3481 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
     3541 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
     3601 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
     3661 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
     3721 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
     3781 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
     3841 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
     3901 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
     3961 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
     4021 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
     4081 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
     4141 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
     4201 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
     4261 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
     4321 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
     4381 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
     4441 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
     4501 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
     4561 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
     4621 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
     4681 ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa gcattttcaa tattggacta
     4741 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
     4801 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
     4861 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
     4921 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
     4981 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
     5041 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
     5101 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
     5161 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
     5221 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
     5281 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
     5341 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
     5401 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
     5461 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
     5521 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
     5581 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
     5641 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
     5701 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
     5761 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
     5821 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
     5881 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
     5941 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
     6001 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
     6061 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
     6121 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
     6181 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
     6241 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
     6301 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
     6361 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
     6421 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
     6481 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
     6541 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
     6601 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
     6661 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
     6721 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
     6781 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
     6841 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
     6901 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
     6961 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
     7021 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
     7081 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
     7141 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
     7201 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
     7261 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
     7321 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
     7381 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
     7441 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
     7501 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
     7561 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
     7621 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
     7681 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
     7741 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
     7801 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
     7861 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
     7921 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
     7981 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
     8041 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
     8101 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
     8161 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
     8221 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
     8281 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
     8341 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
     8401 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
     8461 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
     8521 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
     8581 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
     8641 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
     8701 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
     8761 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
     8821 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
     8881 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
     8941 aacataaata cagtattatt tcggttgctt gctgcttcac ctagcccttg tactcgtgtt
     9001 tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc taaaagcaaa
     9061 atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg cataacaagt
     9121 cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac gataggtgca
     9181 gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt tccccaattg
     9241 taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac
     9301 atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac taaatgcggc
     9361 aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac agaacgcggc
     9421 attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa taaatagatc
     9481 atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag caaaatcccc
     9541 acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg tcccgctagt
     9601 accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa
     9661 gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat
     9721 aacgctgcca ttcgaatgat caattttgga cgatccacca cgtcccctaa aggaactaaa
     9781 aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc gactgcactt
     9841 gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta atacatattg
     9901 gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt taaaggtttt
     9961 ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac ttatggaaaa
    10021 gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt ttgttttcct
    10081 tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac agtaacactt
    10141 gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc accacacgca
    10201 tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg ctgtagaggc
    10261 aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg tatcatatag
    10321 cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac ccaatgattc
    10381 gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc agtgatgtta
    10441 ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa gctcaaacca
    10501 gcaatgactc caactactga agtaggaata cctggaacag cacacatcac cgctgtcaat
    10561 acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc
    10621 ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa atacaaccga
    10681 tagcccacta ccagtatcag aatgattccc aacgagatca atatcaatcg gattattttc
    10741 tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt aacataaaaa
    10801 caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa aaattattta
    10861 acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc ttcttcttta
    10921 gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc ttctttcaag
    10981 cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc tccagctgca
    11041 gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc agcagcagct
    11101 acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt aactaggtcg
    11161 ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat gttcaatgcc
    11221 attataattt cctccgttta attgttcttt tttatttaat gctttatgcg gcaattaagc
    11281 tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt tgcggactgg
    11341 cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca attttgccaa
    11401 tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt tgatttctaa
    11461 tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta catcttcgtt
    11521 actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt
    11581 tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt tagcatcacg
    11641 aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt caacgactac
    11701 tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg ctttttttgc
    11761 gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa gagttttttg
    11821 cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat tcatcaccta
    11881 ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc
    11941 tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa taaaaaactc
    12001 tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc tcggtaggaa
    12061 attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc tcaactagct
    12121 tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc aacattctaa
    12181 tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt gttgtgatcg
    12241 ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc aatacatcat
    12301 ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt ccgattggta
    12361 catggatatt tccagctttg tccacacggt aagttacttt accagctttc acttcgttta
    12421 ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt aagccttttg
    12481 gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt gcaacaacta
    12541 catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca cctacgaaat
    12601 ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact aaaacagttt
    12661 gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg tctgcttttt
    12721 tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt gcaatgtttg
    12781 tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa tcaacttttt
    12841 ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct tgattgtggt
    12901 tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa ctgagaagct
    12961 gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc agttccttca
    13021 accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt taattcagcg
    13081 atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg ttcgcctgaa
    13141 cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt ttttgtaacg
    13201 aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa acctgcttga
    13261 tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc cgcttgacct
    13321 agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg caatttaacg
    13381 attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg tggttaattg
    13441 gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga caaataatgt
    13501 gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa gatttttttc
    13561 tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa caacaatttt
    13621 tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca gatgaaagca
    13681 aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa aatgaggtgg
    13741 agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct gtgatgattg
    13801 gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata cgtagtatat
    13861 ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca aaaacctatc
    13921 gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa cccgatgatg
    13981 aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc ttgtttgttc
    14041 ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa aaaggaacac
    14101 gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt tctgaattga
    14161 atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc caccaagatg
    14221 ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg aatatcggca
    14281 ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt gaagtagatg
    14341 atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa
    14401 attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag
    14461 caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa atgagtggcc
    14521 gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa
    14581 tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt gatgcaaaaa
    14641 aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc ttaaaagctg
    14701 tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt tgtgctacac
    14761 cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga gatcggcttg
    14821 atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc gggttagtca
    14881 tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa gttggttctg
    14941 caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct catcaagcag
    15001 cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat cccgttgcag
    15061 gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa gcttttatct
    15121 ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg
    15181 caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca gaaggtggct
    15241 tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag agggcgtgat
    15301 aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa aacgaagaga
    15361 ggactaattg cccttctctt cgttttttat taatccatca atttcgtatt ttttacttca
    15421 tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt caggtgttca
    15481 aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac tggtcggcca
    15541 atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc tcgacggaca
    15601 ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt tggcaatact
    15661 tcaaaggacg caggacctcc atctagttga cgtccaccat gattggatac ccagatacca
    15721 tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc ttttacgata
    15781 actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt
    15841 gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc actttgttcg
    15901 ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat gacgtcttct
    15961 tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc ccctgcttct
    16021 actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata taattggaaa
    16081 aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg
    16141 ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc ttcccctttt
    16201 tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc taattcaatt
    16261 ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg tggcatgatt
    16321 ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc agaaccaccg
    16381 cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc tagttaggca
    16441 atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat agtagatcct
    16501 tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta agcaaacatt
    16561 tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa gctgatctac
    16621 ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta ctttttcctc
    16681 tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt ttaacggtac
    16741 tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa atcgagtggt
    16801 tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa cgacagcagg
    16861 cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat cattaaaagg
    16921 cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc catacgttcc
    16981 taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga ctttatctat
    17041 gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt gattccactc
    17101 gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt ttccagacaa
    17161 atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt cttttactgc
    17221 ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg tcccgctgta
    17281 gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg attctgccgt
    17341 tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac cacttaacgc
    17401 aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat ttttgattgg
    17461 ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa ggccaatcat
    17521 atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg aaatcaagtt
    17581 ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct ttttctccgt
    17641 caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt ttcacgactt
    17701 cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa cgcaagatat
    17761 gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga
    17821 atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc atgatcattt
    17881 caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc ccatttttga
    17941 cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc attttcatac
    18001 tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa tatgtatgca
    18061 agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat tgtaaaataa
    18121 aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa aagtacgttg
    18181 tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt atccattacg
    18241 taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc tttacggagt
    18301 tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt tagaaatttc
    18361 atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta tattgattac
    18421 aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca ctgactgatt
    18481 ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc tttgccattt
    18541 acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac gtaacttttt
    18601 acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga tatgattaat
    18661 ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa aacacttgta
    18721 tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga agcattaaaa
    18781 attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg taatattttc
    18841 cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg tgatatgacg
    18901 cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga tacttctttc
    18961 ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga cagtgaaaat
    19021 catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca atcttggagc
    19081 gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg agatttggct
    19141 gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac tcttgatatc
    19201 gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac tgaagcgaaa
    19261 gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt agaaattgaa
    19321 aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt
    19381 cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga tctaaataat
    19441 acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc gtgccatagg
    19501 ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt cccatgctga
    19561 tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact ttgttttgca
    19621 ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc acatccatga
    19681 taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc caaagcaatt
    19741 tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg atcggccctg
    19801 aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg atgccattta
    19861 caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct cctcttttct
    19921 cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca aacaataatt
    19981 ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag caaaagctta
    20041 aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg taaggggtca
    20101 taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg ttgtgaaact
    20161 taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa cggtctagca
    20221 agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac gtaagtaatg
    20281 gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt tcattttcct
    20341 agacttctgc tataataacc aagaattaat tttgtaacag atttttttga aaaaacaagt
    20401 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
    20461 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
    20521 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
    20581 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
    20641 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
    20701 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
    20761 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
    20821 tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
    20881 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
    20941 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
    21001 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
    21061 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
    21121 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
    21181 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
    21241 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
    21301 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
    21361 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
    21421 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
    21481 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
    21541 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
    21601 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
    21661 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
    21721 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
    21781 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
    21841 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
    21901 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
    21961 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
    22021 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
    22081 gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat gacgctgaaa
    22141 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
    22201 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
    22261 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
    22321 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
    22381 cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt aaagtctgca
    22441 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
    22501 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
    22561 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
    22621 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
    22681 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
    22741 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
    22801 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagttt
//
LOCUS       DACDID010000030        22749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDID010000030 DACDID010000000
VERSION     DACDID010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.030"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..444
                     /locus_tag="IV236_000908"
     CDS             34..444
                     /locus_tag="IV236_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8771122.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            437..1138
                     /locus_tag="IV236_000909"
     CDS             437..1138
                     /locus_tag="IV236_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8771123.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1138..2724
                     /locus_tag="IV236_000910"
     CDS             1138..2724
                     /locus_tag="IV236_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8771124.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2743..2964
                     /locus_tag="IV236_000911"
     CDS             2743..2964
                     /locus_tag="IV236_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8771125.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2961..3722
                     /locus_tag="IV236_000912"
     CDS             2961..3722
                     /locus_tag="IV236_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8771126.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4102..4611
                     /locus_tag="IV236_000913"
     CDS             4102..4611
                     /locus_tag="IV236_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8771127.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4686..5225)
                     /locus_tag="IV236_000914"
     CDS             complement(4686..5225)
                     /locus_tag="IV236_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8771128.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5365..5976)
                     /gene="rpsD"
                     /locus_tag="IV236_000915"
     CDS             complement(5365..5976)
                     /gene="rpsD"
                     /locus_tag="IV236_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8771129.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6292..7089
                     /locus_tag="IV236_000916"
     CDS             6292..7089
                     /locus_tag="IV236_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8771130.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7117..7752
                     /locus_tag="IV236_000917"
     CDS             7117..7752
                     /locus_tag="IV236_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8771131.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7772..8545
                     /locus_tag="IV236_000918"
     CDS             7772..8545
                     /locus_tag="IV236_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8771132.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8953..9750
                     /locus_tag="IV236_000919"
     CDS             8953..9750
                     /locus_tag="IV236_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8771133.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9728..10141
                     /locus_tag="IV236_000920"
     CDS             9728..10141
                     /locus_tag="IV236_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771134.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10125..12770
                     /gene="mutS"
                     /locus_tag="IV236_000921"
     CDS             10125..12770
                     /gene="mutS"
                     /locus_tag="IV236_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8771135.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12788..14896
                     /gene="mutL"
                     /locus_tag="IV236_000922"
     CDS             12788..14896
                     /gene="mutL"
                     /locus_tag="IV236_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8771136.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14918..15478
                     /locus_tag="IV236_000923"
     CDS             14918..15478
                     /locus_tag="IV236_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8771137.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15563..17551)
                     /gene="treP"
                     /locus_tag="IV236_000924"
     CDS             complement(15563..17551)
                     /gene="treP"
                     /locus_tag="IV236_000924"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8771138.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17774..18487
                     /gene="treR"
                     /locus_tag="IV236_000925"
     CDS             17774..18487
                     /gene="treR"
                     /locus_tag="IV236_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8771139.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18564..19010
                     /gene="msrB"
                     /locus_tag="IV236_000926"
     CDS             18564..19010
                     /gene="msrB"
                     /locus_tag="IV236_000926"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8771140.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19180..19782
                     /gene="ruvA"
                     /locus_tag="IV236_000927"
     CDS             19180..19782
                     /gene="ruvA"
                     /locus_tag="IV236_000927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8771141.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19795..20796
                     /gene="ruvB"
                     /locus_tag="IV236_000928"
     CDS             19795..20796
                     /gene="ruvB"
                     /locus_tag="IV236_000928"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8771142.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20825..21403
                     /locus_tag="IV236_000929"
     CDS             20825..21403
                     /locus_tag="IV236_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8771143.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21455..21937
                     /locus_tag="IV236_000930"
     CDS             21455..21937
                     /locus_tag="IV236_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8771144.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22054..22428
                     /locus_tag="IV236_000931"
     CDS             22054..22428
                     /locus_tag="IV236_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771145.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7402 a         4070 c         4921 g         6356 t
ORIGIN      
        1 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
       61 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
      121 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
      181 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
      241 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
      301 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
      361 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
      421 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
      481 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
      541 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
      601 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
      661 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
      721 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
      781 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
      841 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
      901 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
      961 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     1021 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     1081 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     1141 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     1201 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     1261 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     1321 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     1381 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     1441 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     1501 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     1561 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     1621 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     1681 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     1741 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     1801 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     1861 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     1921 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     1981 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     2041 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     2101 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     2161 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     2221 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     2281 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     2341 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     2401 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     2461 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     2521 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     2581 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     2641 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     2701 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     2761 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     2821 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     2881 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     2941 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     3001 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     3061 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     3121 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     3181 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     3241 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     3301 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     3361 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     3421 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     3481 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     3541 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     3601 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     3661 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     3721 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     3781 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     3841 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     3901 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     3961 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     4021 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     4081 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     4141 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     4201 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     4261 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     4321 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     4381 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     4441 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     4501 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     4561 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     4621 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     4681 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     4741 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     4801 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     4861 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     4921 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     4981 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     5041 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     5101 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     5161 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     5221 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     5281 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     5341 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     5401 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     5461 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     5521 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     5581 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     5641 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     5701 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     5761 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     5821 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     5881 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     5941 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     6001 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     6061 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     6121 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     6181 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     6241 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     6301 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     6361 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     6421 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     6481 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     6541 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     6601 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     6661 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     6721 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     6781 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     6841 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     6901 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     6961 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     7021 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     7081 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     7141 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     7201 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     7261 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     7321 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     7381 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     7441 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     7501 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     7561 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     7621 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     7681 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     7741 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     7801 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     7861 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     7921 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     7981 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     8041 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     8101 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     8161 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     8221 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     8281 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     8341 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     8401 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     8461 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     8521 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     8581 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     8641 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     8701 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     8761 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     8821 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     8881 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
     8941 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
     9001 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
     9061 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
     9121 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
     9181 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
     9241 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
     9301 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
     9361 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
     9421 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
     9481 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
     9541 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
     9601 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
     9661 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
     9721 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
     9781 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
     9841 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
     9901 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
     9961 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    10021 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    10081 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    10141 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    10201 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    10261 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    10321 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    10381 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    10441 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    10501 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    10561 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    10621 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    10681 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    10741 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    10801 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    10861 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    10921 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    10981 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    11041 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    11101 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    11161 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    11221 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    11281 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    11341 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    11401 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    11461 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    11521 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    11581 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    11641 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    11701 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    11761 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    11821 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    11881 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    11941 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    12001 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    12061 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    12121 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    12181 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    12241 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttccact cattatcatg
    12301 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    12361 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    12421 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    12481 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    12541 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    12601 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    12661 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    12721 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    12781 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    12841 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    12901 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    12961 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    13021 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    13081 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    13141 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    13201 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    13261 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    13321 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    13381 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    13441 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    13501 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    13561 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    13621 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    13681 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    13741 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    13801 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    13861 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    13921 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    13981 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    14041 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    14101 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    14161 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    14221 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    14281 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    14341 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    14401 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    14461 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    14521 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    14581 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    14641 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    14701 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    14761 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    14821 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    14881 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    14941 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    15001 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    15061 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    15121 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    15181 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    15241 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    15301 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    15361 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    15421 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    15481 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    15541 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    15601 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    15661 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    15721 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    15781 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    15841 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    15901 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    15961 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    16021 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    16081 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    16141 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    16201 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    16261 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    16321 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    16381 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    16441 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    16501 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    16561 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    16621 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    16681 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    16741 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    16801 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    16861 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    16921 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    16981 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    17041 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    17101 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    17161 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    17221 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    17281 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    17341 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    17401 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    17461 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    17521 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    17581 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    17641 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    17701 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    17761 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    17821 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    17881 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    17941 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    18001 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    18061 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    18121 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    18181 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    18241 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    18301 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    18361 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    18421 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    18481 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    18541 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    18601 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    18661 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    18721 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    18781 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    18841 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    18901 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    18961 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    19021 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    19081 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    19141 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    19201 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    19261 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    19321 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    19381 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    19441 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    19501 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    19561 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    19621 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    19681 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    19741 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    19801 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    19861 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    19921 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    19981 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    20041 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    20101 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    20161 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    20221 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    20281 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    20341 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    20401 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    20461 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    20521 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    20581 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    20641 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    20701 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    20761 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    20821 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    20881 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    20941 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    21001 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    21061 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    21121 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    21181 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    21241 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    21301 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    21361 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    21421 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    21481 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    21541 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    21601 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    21661 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    21721 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    21781 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    21841 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    21901 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    21961 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    22021 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    22081 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    22141 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    22201 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    22261 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    22321 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    22381 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    22441 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    22501 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    22561 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    22621 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    22681 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    22741 ttaagtttt
//
LOCUS       DACDID010000031        22705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDID010000031 DACDID010000000
VERSION     DACDID010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.031"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IV236_000932"
     CDS             133..3660
                     /locus_tag="IV236_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8771146.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IV236_000933"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IV236_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8771147.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IV236_000934"
     CDS             7448..7789
                     /locus_tag="IV236_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8771148.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IV236_000935"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IV236_000935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8771149.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IV236_000936"
     CDS             9146..11566
                     /locus_tag="IV236_000936"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8771150.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV236_000937"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV236_000937"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8771151.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IV236_000938"
     CDS             13000..13737
                     /locus_tag="IV236_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8771152.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IV236_000939"
     CDS             13751..14566
                     /locus_tag="IV236_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8771153.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IV236_000940"
     CDS             14579..15226
                     /locus_tag="IV236_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8771154.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IV236_000941"
     CDS             15242..15898
                     /locus_tag="IV236_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8771155.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IV236_000942"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IV236_000942"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8771156.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IV236_000943"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IV236_000943"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8771157.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IV236_000944"
     CDS             18240..18758
                     /locus_tag="IV236_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8771158.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IV236_000945"
     CDS             18865..19365
                     /locus_tag="IV236_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8771159.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IV236_000946"
     CDS             complement(19645..20565)
                     /locus_tag="IV236_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8771160.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IV236_000947"
     CDS             20730..21743
                     /locus_tag="IV236_000947"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8771161.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV236_000948"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV236_000948"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8771162.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7567 a         3955 c         4829 g         6354 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaa
//
LOCUS       DACDID010000032        22699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDID010000032 DACDID010000000
VERSION     DACDID010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.032"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..432)
                     /locus_tag="IV236_000949"
     CDS             complement(64..432)
                     /locus_tag="IV236_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8771163.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            603..1559
                     /locus_tag="IV236_000950"
     CDS             603..1559
                     /locus_tag="IV236_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8771164.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1632..1748
                     /locus_tag="IV236_000951"
     CDS             1632..1748
                     /locus_tag="IV236_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8771165.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1803..3485)
                     /gene="recN"
                     /locus_tag="IV236_000952"
     CDS             complement(1803..3485)
                     /gene="recN"
                     /locus_tag="IV236_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8771166.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3503..3952)
                     /locus_tag="IV236_000953"
     CDS             complement(3503..3952)
                     /locus_tag="IV236_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8771167.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4059..4877)
                     /locus_tag="IV236_000954"
     CDS             complement(4059..4877)
                     /locus_tag="IV236_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8771168.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4886..5776)
                     /locus_tag="IV236_000955"
     CDS             complement(4886..5776)
                     /locus_tag="IV236_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8771169.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5778..6002)
                     /locus_tag="IV236_000956"
     CDS             complement(5778..6002)
                     /locus_tag="IV236_000956"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8771170.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6007..7344)
                     /locus_tag="IV236_000957"
     CDS             complement(6007..7344)
                     /locus_tag="IV236_000957"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8771171.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7345..8199)
                     /locus_tag="IV236_000958"
     CDS             complement(7345..8199)
                     /locus_tag="IV236_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8771172.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8296..8745)
                     /gene="nusB"
                     /locus_tag="IV236_000959"
     CDS             complement(8296..8745)
                     /gene="nusB"
                     /locus_tag="IV236_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8771173.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8738..9163)
                     /locus_tag="IV236_000960"
     CDS             complement(8738..9163)
                     /locus_tag="IV236_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8771174.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9409..10473)
                     /locus_tag="IV236_000961"
     CDS             complement(9409..10473)
                     /locus_tag="IV236_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8771175.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10645..10890)
                     /locus_tag="IV236_000962"
     CDS             complement(10645..10890)
                     /locus_tag="IV236_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8771176.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11029..11403)
                     /locus_tag="IV236_000963"
     CDS             complement(11029..11403)
                     /locus_tag="IV236_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8771177.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11486..12400)
                     /locus_tag="IV236_000964"
     CDS             complement(11486..12400)
                     /locus_tag="IV236_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8771178.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12421..13224)
                     /locus_tag="IV236_000965"
     CDS             complement(12421..13224)
                     /locus_tag="IV236_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8771179.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13257..14246)
                     /gene="manX"
                     /locus_tag="IV236_000966"
     CDS             complement(13257..14246)
                     /gene="manX"
                     /locus_tag="IV236_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8771180.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14349..14840)
                     /locus_tag="IV236_000967"
     CDS             complement(14349..14840)
                     /locus_tag="IV236_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8771181.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15031..17865)
                     /locus_tag="IV236_000968"
     CDS             complement(15031..17865)
                     /locus_tag="IV236_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8771182.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18272..18565)
                     /gene="rpmA"
                     /locus_tag="IV236_000969"
     CDS             complement(18272..18565)
                     /gene="rpmA"
                     /locus_tag="IV236_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8771183.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18578..18922)
                     /locus_tag="IV236_000970"
     CDS             complement(18578..18922)
                     /locus_tag="IV236_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8771184.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18949..19257)
                     /gene="rplU"
                     /locus_tag="IV236_000971"
     CDS             complement(18949..19257)
                     /gene="rplU"
                     /locus_tag="IV236_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8771185.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            19539..20180
                     /locus_tag="IV236_000972"
     CDS             19539..20180
                     /locus_tag="IV236_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8771186.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20242..21873)
                     /locus_tag="IV236_000973"
     CDS             complement(20242..21873)
                     /locus_tag="IV236_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8771187.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21996..22355)
                     /locus_tag="IV236_000974"
     CDS             complement(21996..22355)
                     /locus_tag="IV236_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8771188.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22514..22587)
                     /locus_tag="IV236_000975"
     tRNA            complement(22514..22587)
                     /locus_tag="IV236_000975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22551..22553),aa:Arg,seq:tct)
BASE COUNT         6636 a         4980 c         3762 g         7321 t
ORIGIN      
        1 tttttttaaa aatcaagctg caaaaagcct tttaaaagac tttttacagc ttttctattt
       61 ttttcatagg taagaaagta tgttcacaat gatcaagaca gcgtacaaga cgattgtcaa
      121 cagaaaaacc aaacgccaag tcatcttgaa ataacgcgga taaataattt cttcataata
      181 atatgcttgg aaaacagcaa tacaaattcc cattagcaaa acagaaataa agaaaaacgg
      241 taagatagaa attgtccctg tatttctaga caatgcatgc ataccgcccc acaaaaaagg
      301 aatcgccaaa tcaggggctt ttattttaaa acgacgattt aacgaaaata attttacaag
      361 aaattggcaa ccaaacaaaa cgattaccgg gtaaatatac caaaacaaga taagtggtgt
      421 gaatgtgccc atgaacgtaa actccttttt tatcgttgct tcaagtatac tgtatatact
      481 ttacacaact gtttcatttt atttaaattt atgcagttat attctatatc ttacaaacac
      541 attccatgta ttccaattcc ctatccgtta taataaaaga aacaggcaac aggaggaaaa
      601 tcatgatcaa atattttaca ttagataatg agaaactgcg taataccgga aaggaaactg
      661 accaaacgat ctggtattgt gtagaacgtc ctaatcaaga agagattcat cagctgacac
      721 atcaatttga aattccgtca gattacatca cttccatatt ggacgatgca gaaaattccc
      781 gtacagaaga attcaatcaa tcaaagttga caaagccagc gttattattg ttacaatatc
      841 cttttgctaa aacaagtcct agcggttatt ttcaagtgga tacatacccg ctctctatta
      901 ttttgacgcc caaaaagaag cttatcacaa ttacgaatca tgaaccactg tttttaaaga
      961 aagtctttag ccagaccttt ccaaaaagtg atatttcgcc taatctcaat atcttctttc
     1021 aattcatttg gcaggtcaca ctctcttaca acagttattt agctacgctt caatctcaat
     1081 gcgatacttt ggaagccaaa cttcaagtat ccactgaaaa caaacaactt tatcaaatca
     1141 tggatatcca aaaaagcctc gtttatttca gagaagcgac ttctgctaat ttagatgcat
     1201 tgaaagaact agcgcaaaat aaacttttcc aagaaaatca tgctttgaaa aatcatttac
     1261 gtgatgtatt ggtggaaaca gagcaggcaa tgactacttc tcgcattcaa ttaaaactag
     1321 tagaacaaat gaatcagact ttttcagcaa tcgtatcgaa taatctgaat aatattatga
     1381 aaattttaac ttctttaacg attattttaa ctataccgac aatcgtcggc agtatctatg
     1441 gaatgaacat taagttacct attgctgaaa gggatgatgc ctttatatgg ctagttctat
     1501 taacattatt tatttgtatc gtaactacct attatttaaa aaaagggaag tttttataaa
     1561 aaagccctct tgtggaaatg gaaaaaagat tgtacacttg aatcaactaa ttaatgacga
     1621 ggttgaaaac aatgcgtgaa aaaaaacaaa caagtacttt tgctaaagta acaaaatttg
     1681 tcatttggac aatgctgatt ttaacgattg gatctgttgt attgactgct gtaatgagtg
     1741 tcatgtaaaa ataaaatata gctatcagct gctgaaaagg acctgaacca ctgcgaggtg
     1801 gctcaggtcc tttttgcgag tttcaatagt tcttttgcat gttcgagtgt gagttttgta
     1861 atctcacttc cagccagcat acgtgcaatt tcttcttctc tttcttttgt tttcaactct
     1921 gaaacagaag tttgcgtacg tccatcaatc actgctttca caatataata ttgatagtcg
     1981 gcgactgcag caacttgagg cagatgagtg atgcacaaaa cttgagagta tttcgatatt
     2041 tgagaaattt tatctgcgat ggcctgtgca actcttccgc tcacacctgt atccacttca
     2101 tcaaaaataa tacttgtcac accttgctca gaagaaaaaa tagttttcaa agcaagcaac
     2161 atccgagaca gttcgccacc agaagcgact ttcaccaaag gcttcagtgg ctctcctgga
     2221 ttggtcgtaa tgtaaaattc gatttggtca aatccatcat gattcaattg tctattttct
     2281 tcttttaaga aacgtacctc aaactcagtg ttttccatat acagactttt caattcatgc
     2341 agaatggatt tttccaattc ttttgcgatt tttttacgcg cttcatgaag atctgcggct
     2401 tgttgctgaa gtagaagctc tttttgctct agttcctctt tcattttgtc caattgacct
     2461 tctgaaaaat cagaagaagc tagctcttcg gtgatttcgt catagtaagc caatattgac
     2521 tcaatggatt cgccgtattt acgtttcaac tgtcggatta attctaaacg ttgcgtaact
     2581 tcttctaaac gactctcatc taactccaat aaatcaatct gacgactcat atcgccgatt
     2641 gcatcttgca gcaaatagta agcactttgg atattttcat aaattgcttc atactccaca
     2701 tctagatggg cgatcccttg cagttctgaa acggccaagc caacaccatc tacactgttt
     2761 tgttcattat tcgtcagtgc tccgtaacct gcagctaatc catcagcaat tttttgataa
     2821 ttgctaagtt tttctctttc ttcttttaat ttctcttctt ctccgacttg caaatcagca
     2881 gcagctattt cttcttgctg gaaatggagc atatctattc tctgaacata ggatttttca
     2941 ttttgctgca cttttcgtac ccttttttcc agctcacgat aagagatata ttcttgctga
     3001 taactctttt tcttctgttg gaaagctaca tctccaaaac gatccaataa agcaagatgt
     3061 gattcaggct ggagcagttc ctgatgttcg ttttgtcctt ggatatctac taaataactg
     3121 ccgattcttc tcagattagc taatgtgata atatgaccat tcactcgaca aacatttttt
     3181 ccagttaagg acatatcccg tcgaacaatc agattgtcct catctgtttc gatccctaat
     3241 tcaaccatca gttcagagaa tccttcttgt tttggtagtt caaacaaccc ttcaagcaca
     3301 catttttctg ctccttgacg tatataatcg cttgaacctc ttcctcctgc taacagacct
     3361 aaagcatcga taataatgga tttacccgca ccagtctctc cggtcagcgc ggtcattcct
     3421 tcgtgaaaag aaagccgcaa ttccggaata atcgcaaagt tagtaatact gatttcttgt
     3481 agcatcacat tcgctcctca acttaaaggt aatgcaggaa gtctttcttc aataaaactg
     3541 cctggtcttc agttcgtgta atcatcaaaa cattatcgtc atcattgata atcgaaaata
     3601 attctttctt gtaacgttta tcaatcaaat ttgctgctgc cgaagcattt cccggcaatg
     3661 ttttcaaaat aacaaatttt tccatttggt caactgccac gaaagcgtct tttaatagtt
     3721 tttccaattt agtatttaca tcttcgtttg tttcagctgg tatgctataa cggtagccgc
     3781 catctgaagc cggtaccttg atcagtttca gttcctttat atctctggaa atcgtagctt
     3841 gagtaacaga tattcctttg cttttcagga tttcaacaaa ttcttcctgc tttcggatat
     3901 cttgctcact caacaatcga gtaatcaaac gatgccgatc tttttttctc attacgtctt
     3961 cgctccttag catattcttc ttaatcaatg atagcttatt tccttggtat aataaagaag
     4021 aatccgcata cttctcactt gattttcaca gttacaattt atttatggtc aaactgttcg
     4081 tgtgcatttg aaaccacttc tgccatatta atagctgaat tgattgttcc gctttcgggt
     4141 actttcctca agtgagccaa gaactcgata tttccttctc cccccgtgat cggagaataa
     4201 cttaattcca gcacatcata tccaccttgt gccgcaaatg acaaaatgtc ttctaaaact
     4261 ttttgatggg tctgagggtc acgaacaatt ccgtttttcc ctacagcttc tctgcctgct
     4321 tcgaattggg gtttgattaa agcaatcact tctccctctt cttttaaaat agcatgtaat
     4381 ggaggcagca tcagttttag tgaaataaaa gaaacatcga taacagcaac ttctggaatt
     4441 cctaaggtaa agtcttcggg ttttgcataa cgaaaattca ctctttccat tacgacgact
     4501 ctaggatctt gtcttatttt ccaagctagc tgattgtagc caacgtctaa agcataactc
     4561 atttttgcgc cattttgcaa agctgcatca gtaaatccgc cagtggatgc accaatatct
     4621 aacaatgttt ttccttcaac agatagatca aatgctttga gtgctttttc taattttaat
     4681 ccccctcgag aaacataagg aagtttttta cctttgactc ttaattcact actcactgga
     4741 attttttctc ccggtttatc aaaccgctcg tttttttcgt tgtaaatcaa accagccatc
     4801 actgaacgtt tggcttgttc tcttgtttca aataatcctt gtttgaccgc taatacatct
     4861 acgcgttctt tttccattct ttccctcatt tctccacatc aaattgcttg atcatctggc
     4921 gcatcagccc gccatcaaaa gaaaaaacgt cctcttctaa tttgtctatg atcttgttgg
     4981 cggataacag ctcaatctcc aacgcctcac gtgtcttttc taggcctaat aaacgcgggt
     5041 aagtattttt ttccatacgt tcatctttac cagctgtttt ccctaatgtt ttcgttgtac
     5101 caatcacatc gagtagatca tctcttatct gaaaagctaa acctaaatgt tcggctaatc
     5161 gttgaagtag caataagatt tcttcaggtt gcttcgctaa tatgccacca gccagcaagg
     5221 cataacgaat caaatgccct gtctttcttt catgaataaa tgctaattct tctaaagaaa
     5281 gctttttcga ttcgccctcg atatctgcag cttgcccagc aaccatacct tgtgatcccg
     5341 cacaaacagc taattgctga agcagcagaa gcttaggaga attacctaag tgtgccatac
     5401 tgattagctg gaatgctcct gtaagcaaac catctccggc caatatagct aatgcttcgc
     5461 cgaatacttt atgattggta ggttttcctc tacgaagatc atcattgtcc atcgcaggca
     5521 aatcatcgtg gatcaaggaa tacgtatgca ccatttctaa agcagctgct acttgataga
     5581 cgggaacatc aataggctca tcaaaggaag caacggccgt taaaagtaat aagggacgta
     5641 ttcttttccc gcctgctttc actgaataac tcatcgcatc atagagtcct ctatccgttg
     5701 tatgctcatc taaaaaagac aagatttctt tttctacagc aggtagatga atagctgaaa
     5761 agtcagttaa tctcatctca gctttcctcg tttccgtcaa aatcaacttc ttcatttgat
     5821 tctgtcatca ttttcgtcaa ggtctcttct gctttagcta atgtatcttg gcaatgtttg
     5881 cttaattcca ttcctcgttt aaacgaatct agagctgatt ctaatggaac gtctccttgt
     5941 tctaactgca tgacaatttt ttctaattct tgcagtgact cttcaaaagt tggattttcc
     6001 atatttttat tctcctttgt ctatctcttg tacttgtcct ttaacgattc catcttgata
     6061 atggatcgtc agcaaatccc cttgatgaat ctcggtgaca ctttttacta cttgatcatt
     6121 taacgtcgta tagctgtagc ctcttcccat gattttcaat ggactcaaca aatcaagaga
     6181 ttgaacagct tgagcaaaac gctgctgctg ttgtttcata tattgttgga ttttctcttc
     6241 taagcgtttt tctagataag cagtttcttg gcgagctgct ttaactcgat aaagcggagc
     6301 cacttgttcc aattggtggc ttaatgcgat cgtttgtttt tctttcgcat gataaatttg
     6361 ctgtgtagct tgatataatc gctgtttcaa ctggtctaat ttgatggtct gaccttcata
     6421 caatctttca ggctgccgaa atacatagga acgtcgagca cgctcgaacc gttcttgttt
     6481 gcgctggatc tggcgtacaa acgcctgctg caatctagct tgccgttctt gaatgcgcat
     6541 caactcttct gacaaaacag gaacagctaa ttctgcagct gctgttggag tggcagcccg
     6601 aacatctgct actaaatcag cgatcgtggt atctgtttca tgtcctacag atgaaatcac
     6661 cggcgtatta gcttgataaa tcgcccgagc aacccgctct tcattaaacg gccataaatc
     6721 ctcaatcgaa ccacccccac gaccgatgat cattgtatcg aaattgccca tctcttctac
     6781 tctctggata ttcctgacaa cattgtccgc tgcttggttt ccttgtacaa ctgttgggaa
     6841 caaaaccaat tggacaattg gatatcttct tttcaccgtc gtgatgatat cgcggataac
     6901 cgctccgctt ggactggtaa ggaccgcgat tcgctttggg aaacgtggca gcattttttt
     6961 aggcccttga aaaaggcctt ccttcatcag cttttctttt cgttcttcca attcttgata
     7021 taaagcacct attccatcag gctccatgtg ctctacatag atttgataag aacctcccgc
     7081 ttcatataat gaaatacgcc caacgacaag gactttcatt ccttcttttg gctggaatcg
     7141 taatttttgg aatgctcctt tgaacataat agcagatatt tttgcatggt catcttttaa
     7201 gctgaaatat tgatgggcat tggcacgtaa acggaaattc gatatttcgc ctgttaaata
     7261 tacgcgttct aaataaggat cggcatcaaa ttttcttttc aaatacttcg ttaatgtagt
     7321 gacagttaaa tattcttgtg ccatctattc acctgctcca aaagcttcgg cagattttac
     7381 ggtttgatac atcagcatcg tgatagtcat tggccctaca ccctttggca caggagtgat
     7441 ccagcctgca ataggctcca cttcatcaaa tttcacatca ccgatcagct taccgttagc
     7501 atcacgattc atccctacat ctatcactac agcacctggt tttacgaatt cttttgtgac
     7561 aaaatgtcca cgtcctattg ctacgactaa aatatctgct tcttgtgcca gttcagccag
     7621 attttcggtt ttagaatgag caacagtaac agttgcatct gccatcaaga gtagctgagc
     7681 catcggttta ccgacgatat tgcttcgtcc gataactact gctcgtttac ccgccggatc
     7741 gatagaataa gcttcaaaca ttttcatgat gccgtatgga gtacaaggaa tcttgtccgg
     7801 tttacccgca aggagtctgc ctaaattcat cggatgaaaa ccgtctacgt ctttttcagg
     7861 gcggatagct aacaggatct tttcttcatc tatatgcttt ggcaatggta attggaccaa
     7921 tataccatga aaactaggat cttgattgta tttttcaatt tctgcaagta gttcgtcttc
     7981 agaaatcgtc tcaggataac gctctaccaa tgaatggatt ccgatttctt ttgctacacg
     8041 ttctttattt cttacataga tttggctggc tggattttcc cctaccaata aaacgactaa
     8101 tccgggatgg ataccattat ctttcaattt ttggattttt tcagcaatct ctgcctgcat
     8161 tttttcagct aattcttttc cgttaatcaa ttctgccatc taaccactcc cagatatttt
     8221 ttgtgttttc attctaccat agaattttcc cgatttataa caggtttatg atgattcata
     8281 gaaaaaagct aggacttacg ccccagcttc taattctttc aatacattgg ataaaacacc
     8341 gttgacgaat ttacgtgaac ggtcatcact aaatgttttt gccaactcga ttgcttcatt
     8401 taaagcaaca gccgctggta catctgtcac ataagtcatt tcaaaaatcg cgatacgcaa
     8461 gatcaccaaa tccatttttg agatccgatt gatgtgccaa ttgtttttta aatgtttttc
     8521 aatcacttta tccaattcat ctttttttgc acagacaccg ccaactagcg tatcaagata
     8581 aacaggaaca aactctgatt catcttcgtt gatcatgtcg cggtggtcta attcgattgc
     8641 gttgaaaatc gcatcttctt ttgttaggtc tgcattgaag tctaatggaa ataaagcctg
     8701 caatgccatt tcccgaatct cgtgtcttga taattcttta ttcattctct tcctcttcgt
     8761 cttcaaaaag ttcttcgatt gttggttgtt ctaatttttc aggaacaaca gctactacat
     8821 ggatattgac ttcagataaa tcgatatccg tcatgaaaag gacttgctgg ctgacccgtt
     8881 cctgcatttc cattgctact ttaggaactg aaacaccata ttctagataa cagtaaagat
     8941 ctactttcaa gccatcttcc tcgtttgcta agtaaacacc ttttccgtga gctgcacgtc
     9001 ctaataattc agtgacattg ctggcaaaag ttccgcgcat tccgtatacg ccttctactt
     9061 tagaagctgc aatcccgata attacttcga tgacttcagg agcgatcacg atctctccta
     9121 aatcttggtt tgcgtttaaa atcaaatttt tttcatctgc catttattta ttcctccctt
     9181 tgcctaagca cgagatacat ccgaattctc tatgatgcgg catagtgatc acgtggtaag
     9241 aaaactgccg ccgatcagct gcaaactgct atgaattgga gaaaacaccc aactttatta
     9301 tctcttacag cttaaaagtt cttctatagt gtatcattaa atcgagtgat tttctataac
     9361 aaagtctgtg aacactgatt tttacgctta cttcttctat tgatgaaact ataagatgat
     9421 caattctttt ggtgaacgag tcaacacacg atttccatct tctgtgatca acaagtcatc
     9481 ctcaatacgt actccaccga ttcctggtaa gtagatccct ggttcatcag tgatcacatt
     9541 tcctggtaca aattgctttt ctgcacgaga agaaacgttt ggtccttcgt ggatctctaa
     9601 accgatccca tgtcctgtag aatgtccaaa tgcttccccg taaccatgtt ttgaaatata
     9661 atctctagca actgcatcta attggacacc agtcattcct ggttttgcag catcaataac
     9721 tgctaattga gcatctaaga ctatttgata aatttctttt aatttttccc caggatcacc
     9781 aatagcgaat gtacgtgtca tatcggaaac gtacccttcg taatagcaac caaagtccaa
     9841 tgtgatcaag tcaccttgtt cgatgatttt tttgcttgct acaccatgcg gcatagccga
     9901 acgcaatccg ctagcaacga tcgtatcaaa agaaacacct gaagcgccaa gagaacgcat
     9961 ataaaagtct agttgatttg cgacttcaat ctcagtcatg cccggttgga tcattttcag
    10021 gatgtgatca tatgccatat ccgcaatatg acatgctttt tcgataatag cgatttcttc
    10081 ttcatctttc acttctctga gttcttcgat catctgtgaa acaggtacta attccgcatc
    10141 gatgatttct tctaaaaccg aatattctaa aaatgagact gttgtttcct caaaagctaa
    10201 tgagtgcagt tcttctttgc gtactaagtt tgctacctca tcaaaaatag gtccgacatt
    10261 tttaataatt tcaaaccctt gagcctgcgc tgccgcctgc tcggtataac gaaaatcagt
    10321 aataaaaaat gctttttcca aagtaataac agctaatcct gtcgtacctg taaagttcgt
    10381 taagtaacga agattgtacg gactggtaac caaaaatgaa tccaaatttt cttcttgcat
    10441 ttttttgcgc aatttttcta cacgtagcat cataatgaaa ctccctcttt ctttgcacgc
    10501 ttttttccat tatagcaaac ttttcttcgt acacgacagt taaataaaaa tttccggaaa
    10561 acttcttttt attttaaata aataaagcca aaaaggagtc acagtctcgc tgcaactcct
    10621 tttagtttac tattttgtaa aaacttatga tcgttttcca aagaaaaatg agacaactgc
    10681 taccaaaatc actgcgccta aaatagatgg aataagtgcc atacctgcag cttttggtcc
    10741 ccattcgccg aaaatcgctt gtccaatact tgaaccgact agtccagcaa tgatattcgt
    10801 tacacagccc atcgaatttc ctttactggt gatcgcacct gcaattgctc cgatcacggc
    10861 tccaacaatc aatgtccata accaactcat aatataacct ccttctttgc taatcaattt
    10921 tagcatatgg gatttaaagg ggacaaataa ttggttttgc tatttcatat tttcttctgc
    10981 aaagtaaaaa aataaagaaa acctcctctt agaagttttc tttatttttt acttgcgttt
    11041 aaacatacta cgaataccac gtttcactac atcaaagaaa ctcaatgatt gtaccattct
    11101 atcttcatct acatattctc tgatcgcacg aagcactttt ttaggttctt tggaagaaaa
    11161 tgtgtatgta ccattttttt tcgtttgaat ggcatatcga ggaatccatt ttccctttag
    11221 catgactgat gctagtacat aatccacttc ttcccatgga atttggatat atttgcgtct
    11281 atctcgcgca tggtaaaact caaaaccatt atcacctatc atgatttgcc cgtaatctgt
    11341 cagacccgta aacgaagtag catcaattac caagtctact tttgtattga ttgattgaac
    11401 cattttctcc ctccagatat ttgtttatga acagtatact atataaaaaa agctcgggac
    11461 aacgcccgag ctatattttt atggattata aaagtccgat gacgtgtcca acaacaccga
    11521 ctacgaataa accaaggatg ataacgattg gagaaacctt tttcttaagc aaccacatac
    11581 ataacaatgt taatcctaat gcagctaaac caggaatcaa ttggtctaag ttgtcttgta
    11641 atgttgttac cttttcagga gaaagggcta agccattatt aacttgttca aatgcactct
    11701 tgattccttc accacctgaa ggcaatttat cccattcgat gtaagcacct ttatctaatt
    11761 ttacagttga tacaactggt aagaatttga ttgataccca tctttgtaca agggcagcta
    11821 atacgaacat ccctaaaatt gaagcacctt ttgttacgtc ttgtaacaat ccgccagaaa
    11881 ggtcatcagt aattttagaa ccagctttgt agccaaattc ttgtgtatac cacatgaatg
    11941 cccaacgaat aatgttccaa cctaagaaga agatgattgg tccaaggata tttcctccca
    12001 tagctaatga agcacctagt gcacctagca ttggacgtac tgtaaaccag aacactgggt
    12061 caccaacacc agctaaagga cccatcatac ctactttaac cccttgaatc gctacatcat
    12121 caacaggcgc gccattcgca cgttcttctt ccagagccaa tgtaacccct aaaattggag
    12181 aagcaatgta tgggtgtgta ttaaagaatt ccaagtgacg ttttaatgct gcagaacgtt
    12241 cttcttttgt tttgtataat tttttgattg ctggaatcat agaaaatgcc cagccaccgt
    12301 tttgcatacg ttcgtagttc catgaacctt gaatgaaggt agagcgccac gcaacagcta
    12361 aacgatcttt ttttgataat ttgatttttt cttctgccat tttattttcc tccttcaaga
    12421 ttaatagtca ttcaaaatgt cgcctaaagg gtcaccagag ttgcttccgc ctccattatt
    12481 tgatgaacca cccattttag aaaggttcaa gtaaataaga gctaatgcaa cacctaaagc
    12541 acctagagca atcaatgtta attgagaaat tgcagctaca acgaaaccaa taacgaagaa
    12601 tggccatact tcttttgtag ccatcatgtt gattaccaaa gcgtaaccta cagctacgac
    12661 cattccccca ccgatagcca taccatctgt taaccatgct ggcattgatt caaggaatga
    12721 ttgcacagtt tgtgctggga taaatagcaa cgctgctgct gggaccgcga tacgaacccc
    12781 ttgtaaacat acagcaatga tgtgccacat ttcaacctta cggatgtttc cttcttcagc
    12841 agccgcatcc atcaaatgaa cgattggaac agcgattgta cgtacgatca ttgttaagaa
    12901 caaccctgca acagccaaag gaacggcaat cgcgatcgca gaaggaacac ctttaacacc
    12961 ttgtccacct aaaactaaaa taattgctga tgctactgat gctaatgcag catctggtgc
    13021 tacagcggca ccgatatttg cccaacctaa agcgatcatc tgaagcgtac cgccaagtac
    13081 gataccagct tcgaggttac cggtaactaa accgatcaat gtacatgcca ctagtggttg
    13141 atgaaattga aattcatcaa gaataccttc cataccagct agaaaggcaa ctaatacgac
    13201 taaaattatt gaaataatag acatgataag cctcctataa attttaatat tatttattac
    13261 gcttttgcta attcagcttt tgcttttttc aaaatatcat ccatgttatc gcgtgaatca
    13321 ttaggtactt tacgtacatc gaatttcaca cctttttgtt caagcttctc aaacgcttca
    13381 acgtcttctt gtcccataga caatacttta cttacaacga ctttaccgac cgaatgcgcc
    13441 attgacccaa catttaattc tttgatgtct acgccacctt caacagctgt caatacgtct
    13501 tcaggatttt caaataaaag caatgctttg gtatttccaa aacgagggtc ttttgctact
    13561 tcgatcattt tgctaatagg aataacattt gcttttacac ctggaggagc agcttgttcg
    13621 attagtctct tacgcagatc atcctttgat actgcatctg aaacaacgat aatacggttt
    13681 ggttgcactg cttttgtcca agctgtcgca acttgtccat gtaacagacg tgagtcaaca
    13741 cgtgctaata catatttgat ttttccgtct ccgaccacag ttccttctgg aagtgctcct
    13801 ttaggttgtg cgtcttcaac tgctgctttc ggtgcttctg ccggttctaa ttcttccggt
    13861 tttactttta caccgtcttt tgctgtttca atgatgtgtg ctgcaatttc atgagcagat
    13921 tccattgaaa agcgtgatgc gtaggcttca atcaacattg gtaaattcaa accgctgacg
    13981 attgcccatt tatctttgtg ctcttcgaat aatgtgtttg attgattgaa cggtgttccg
    14041 ccccataaat caactaagaa taacacttcg tcttgattat caaaagaagc aaccgcttct
    14101 tccaatttgg ctctcaagtc atctggacct tcacttggtt tcaaaatcac ggctttgacg
    14161 ttttcttgtt cgccaaagat cattgaaccg gattgtaaga ttccttcagc aaattgtcca
    14221 tgacttgcta ggataatccc taccatattt atacctccta tttacttttg ttgtaacaaa
    14281 ttcaaaaata ttaataattg ctaaccatta caacagaatt atttgacctt ttccaacaac
    14341 cattcttgct acaccttttt gattttcgct agtagttcca tcaaaaggat tttcctgtca
    14401 gcaggtactt ttcgaatctc taactccact tcttgactgt tcaagtaacg aaaggaatca
    14461 atatcttgtt gatttactga aacaaaattg gtaatcattg atttgccttc agtaaaactc
    14521 ataccgccga tattcaacga cttcaactgt acaccgcagc ggacaaccgc ttcaacatct
    14581 tggggattgg tgaataaaag catcactttt gctcgtgata ataatccact atgatagatt
    14641 tgaatcattt tctgcacggt tattacattt gctgtgatcc ccggcggtgc agcttctttt
    14701 agtagaaact ttctcaattt atcttgagcg actacatcgc tgatcacaat gatacgttca
    14761 acacctgttt gcttcgacca aactgttgcg acttgcccgt ggattaaacg atcatctata
    14821 cgtgccaatc gaatatccat ctgcaaaact ccttcttcct cactacttca cagaaacccc
    14881 tatcatttct gtgtgaacaa ccacctccta gcacacacta ctataagcaa aaagcgtgcc
    14941 aactttttta cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat
    15001 acactataaa agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa
    15061 ctgattatgg actagacgta gcagataata catttcttct atcggcactt ttacttttat
    15121 ctgatcttcc aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt
    15181 atttgtcaat tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat
    15241 tcctgctgtg tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt
    15301 cgtctgagag atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat
    15361 aaagatgaag ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag
    15421 ggagatgaat tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc
    15481 gatattttga tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt
    15541 aatcccagta gtagctatga tctgatattc tttttggata actggcagtt cttctttcac
    15601 ttccacaatc gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg
    15661 ttcgatcaat tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac
    15721 agcttttttc ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc
    15781 gtaacctcga aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga
    15841 gacctttcgt acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg
    15901 aattccagtg tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa
    15961 gagtatgacc ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc
    16021 tgttttcgga tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa
    16081 ctgttggaca acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac
    16141 accgattctg ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata
    16201 gtcttcactt tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt
    16261 cgcaacagca tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg
    16321 ccgttcttct cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata
    16381 aataaagttt tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt
    16441 ggtcacttct atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga
    16501 aaaaccatgg tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg
    16561 attgattgct tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat
    16621 atcatataga ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt
    16681 tggtgctaca gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt
    16741 gcttgctaat tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga
    16801 gatttcgggt gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga
    16861 cttcaattgt cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc
    16921 tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa
    16981 atcgactttc tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat
    17041 acgccgaaca aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat
    17101 ccgtacattt gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc
    17161 cataaagtaa aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa
    17221 cagcataccg ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa
    17281 agcacctttt gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc
    17341 agcacaattg aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa
    17401 atgatacatc gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca
    17461 atttaatcct cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat
    17521 gctgccgtta gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt
    17581 tttttcttca gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac
    17641 gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt
    17701 tccttcgcgc actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac
    17761 ttcagcaatt tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt
    17821 tttttctttg acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt
    17881 tctatagact attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt
    17941 caactattta cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt
    18001 aaaagaacga aaaatcccga tacgattttt ataatggaaa caagaaaaag aacactgttt
    18061 accatataat tatttcactg atagcaaaca atatagcttc catcaattga aaacgatagt
    18121 tatcaaaaca tcctgattcc aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa
    18181 acttcgtagc ctccactaca acttttattt ttataaacaa aaaaggctgt gacaaaagtc
    18241 ttgtcacaac ctctttaatc ataagattca attaagcttc ttgagctact ggataaacag
    18301 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    18361 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    18421 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    18481 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    18541 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    18601 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    18661 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    18721 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    18781 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    18841 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    18901 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    18961 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    19021 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    19081 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    19141 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    19201 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    19261 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    19321 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    19381 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    19441 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    19501 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    19561 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    19621 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    19681 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    19741 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    19801 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    19861 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    19921 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    19981 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    20041 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    20101 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    20161 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    20221 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    20281 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    20341 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    20401 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    20461 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    20521 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    20581 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    20641 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    20701 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    20761 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    20821 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    20881 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    20941 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    21001 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    21061 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    21121 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    21181 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    21241 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    21301 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    21361 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    21421 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    21481 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    21541 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    21601 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    21661 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    21721 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    21781 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    21841 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaattt
    21901 tctaaaatac cattcattag aatatcatat ttcatgagat aaagtaatat ttttcacaaa
    21961 gtttcctgtt agataaattc ttattttttc tatatttata agaaaataga aagcaatgcc
    22021 aacagagcta gcccaccttg tttgagtaaa atccaacgat cgctagtcaa actgccatat
    22081 aatgcaacaa aaaaaataga tattaatagc atacttgtga tttctttaga tgcggaagaa
    22141 agataagctc cataaagtag tcccactcct aatagtccat tgtacactcc ttggttctta
    22201 aacaatgttt gaacagattt tctgcttaac tcttctttac tcattttaaa aaccctactt
    22261 gtcgtgtcag aatcagttct caccgtttcg atatacatga tataaaaaaa ttcaattgca
    22321 actagagtca ctaaaattct agatagtatc gtcatatctt cttcctcttt tctgatgagt
    22381 tttatttttt tgctccttat aattgtactc gataaggatg tttttttatt ttatttgcct
    22441 ttgaaaacaa acaatattgg cacaaaaaac aaagaaaaac cgttagaaac ctcagtttct
    22501 aacggttttt tagcgtcaca ggagggattc gaacccccga ccgtccgctt agaaggcgga
    22561 tgctctatcc agctgagcta ctatgacaag aatcagcgga acatcaataa ttatagataa
    22621 ttaaattgct tctgtcaata cgaaatagtt atttaattag caaataatga attattttgg
    22681 cccaactact aaatcaatc
//
LOCUS       DACDID010000033        22596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDID010000033 DACDID010000000
VERSION     DACDID010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.033"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(314..1162)
                     /locus_tag="IV236_000976"
     CDS             complement(314..1162)
                     /locus_tag="IV236_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8771189.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1326..2228
                     /gene="gnd"
                     /locus_tag="IV236_000977"
     CDS             1326..2228
                     /gene="gnd"
                     /locus_tag="IV236_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8771190.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2230..3231
                     /locus_tag="IV236_000978"
     CDS             2230..3231
                     /locus_tag="IV236_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8771191.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3228..3512
                     /locus_tag="IV236_000979"
     CDS             3228..3512
                     /locus_tag="IV236_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8771192.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3545..4342
                     /locus_tag="IV236_000980"
     CDS             3545..4342
                     /locus_tag="IV236_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8771193.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4356..5189
                     /locus_tag="IV236_000981"
     CDS             4356..5189
                     /locus_tag="IV236_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8771194.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5207..6064
                     /locus_tag="IV236_000982"
     CDS             5207..6064
                     /locus_tag="IV236_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8771195.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6086..6595
                     /locus_tag="IV236_000983"
     CDS             6086..6595
                     /locus_tag="IV236_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8771196.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6632..7033
                     /locus_tag="IV236_000984"
     CDS             6632..7033
                     /locus_tag="IV236_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771197.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7145..7498
                     /locus_tag="IV236_000985"
     CDS             7145..7498
                     /locus_tag="IV236_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771198.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7532..7975
                     /locus_tag="IV236_000986"
     CDS             7532..7975
                     /locus_tag="IV236_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8771199.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7992..9077
                     /gene="uxuA"
                     /locus_tag="IV236_000987"
     CDS             7992..9077
                     /gene="uxuA"
                     /locus_tag="IV236_000987"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8771200.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9070..9783
                     /locus_tag="IV236_000988"
     CDS             9070..9783
                     /locus_tag="IV236_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8771201.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10535..10735
                     /locus_tag="IV236_000989"
     CDS             10535..10735
                     /locus_tag="IV236_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8771202.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            11303..11866
                     /locus_tag="IV236_000990"
     CDS             11303..11866
                     /locus_tag="IV236_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771203.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(11927..12292)
                     /locus_tag="IV236_000991"
     CDS             complement(11927..12292)
                     /locus_tag="IV236_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771204.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12368..12613)
                     /locus_tag="IV236_000992"
     CDS             complement(12368..12613)
                     /locus_tag="IV236_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8771205.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(12683..12937)
                     /locus_tag="IV236_000993"
     CDS             complement(12683..12937)
                     /locus_tag="IV236_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771206.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(13101..13424)
                     /locus_tag="IV236_000994"
     CDS             complement(13101..13424)
                     /locus_tag="IV236_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771207.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            13611..13684
                     /locus_tag="IV236_000995"
     tRNA            13611..13684
                     /locus_tag="IV236_000995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13645..13647,aa:Ile,seq:cat)
     gene            complement(14069..14566)
                     /locus_tag="IV236_000996"
     CDS             complement(14069..14566)
                     /locus_tag="IV236_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8771208.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(14606..15415)
                     /locus_tag="IV236_000997"
     CDS             complement(14606..15415)
                     /locus_tag="IV236_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8771209.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            15749..16606
                     /locus_tag="IV236_000998"
     CDS             15749..16606
                     /locus_tag="IV236_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8771210.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            16599..17501
                     /locus_tag="IV236_000999"
     CDS             16599..17501
                     /locus_tag="IV236_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8771211.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            17534..17884
                     /locus_tag="IV236_001000"
     CDS             17534..17884
                     /locus_tag="IV236_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8771212.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            17918..18856
                     /locus_tag="IV236_001001"
     CDS             17918..18856
                     /locus_tag="IV236_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8771213.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            18934..19827
                     /locus_tag="IV236_001002"
     CDS             18934..19827
                     /locus_tag="IV236_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8771214.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            19865..20428
                     /gene="amaP"
                     /locus_tag="IV236_001003"
     CDS             19865..20428
                     /gene="amaP"
                     /locus_tag="IV236_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8771215.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            20442..20630
                     /locus_tag="IV236_001004"
     CDS             20442..20630
                     /locus_tag="IV236_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8771216.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            20643..21545
                     /locus_tag="IV236_001005"
     CDS             20643..21545
                     /locus_tag="IV236_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8771217.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            21577..21819
                     /locus_tag="IV236_001006"
     CDS             21577..21819
                     /locus_tag="IV236_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8771218.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(22075..22446)
                     /locus_tag="IV236_001007"
     CDS             complement(22075..22446)
                     /locus_tag="IV236_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771219.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         7808 a         3688 c         4561 g         6539 t
ORIGIN      
        1 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
       61 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
      121 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
      181 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
      241 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
      301 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
      361 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
      421 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
      481 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
      541 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
      601 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
      661 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
      721 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
      781 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
      841 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
      901 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
      961 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
     1021 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
     1081 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
     1141 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
     1201 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
     1261 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
     1321 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
     1381 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
     1441 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
     1501 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
     1561 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
     1621 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
     1681 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
     1741 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
     1801 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
     1861 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
     1921 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
     1981 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
     2041 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
     2101 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
     2161 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
     2221 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
     2281 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
     2341 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
     2401 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
     2461 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
     2521 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
     2581 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
     2641 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
     2701 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
     2761 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
     2821 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
     2881 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
     2941 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
     3001 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
     3061 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
     3121 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
     3181 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
     3241 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
     3301 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
     3361 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
     3421 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
     3481 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
     3541 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
     3601 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
     3661 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
     3721 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
     3781 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
     3841 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
     3901 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
     3961 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
     4021 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
     4081 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
     4141 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
     4201 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
     4261 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
     4321 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
     4381 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
     4441 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
     4501 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
     4561 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
     4621 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
     4681 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
     4741 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
     4801 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
     4861 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
     4921 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
     4981 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
     5041 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
     5101 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
     5161 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
     5221 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
     5281 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
     5341 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
     5401 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
     5461 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
     5521 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
     5581 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
     5641 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
     5701 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
     5761 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
     5821 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
     5881 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
     5941 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
     6001 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
     6061 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
     6121 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
     6181 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
     6241 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
     6301 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
     6361 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
     6421 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
     6481 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
     6541 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
     6601 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
     6661 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
     6721 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
     6781 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
     6841 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
     6901 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
     6961 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
     7021 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
     7081 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
     7141 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
     7201 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
     7261 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
     7321 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
     7381 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
     7441 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
     7501 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
     7561 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
     7621 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
     7681 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
     7741 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
     7801 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
     7861 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
     7921 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
     7981 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
     8041 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
     8101 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
     8161 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
     8221 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
     8281 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
     8341 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
     8401 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
     8461 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
     8521 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
     8581 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
     8641 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
     8701 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
     8761 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
     8821 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
     8881 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
     8941 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
     9001 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
     9061 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
     9121 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
     9181 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
     9241 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
     9301 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
     9361 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
     9421 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
     9481 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
     9541 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
     9601 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
     9661 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
     9721 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
     9781 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
     9841 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
     9901 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
     9961 agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc ttgcactgct
    10021 tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa aacgacaaat
    10081 agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata gaatgtcata
    10141 gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat aacgaaattc
    10201 catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa
    10261 gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg gttattcaac
    10321 tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt gcattatcct
    10381 tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa cgtaatttga
    10441 gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg caacgaaaaa
    10501 acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag taaaatggtt
    10561 caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg tatttgcaca
    10621 tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag cagtgtctta
    10681 cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag cttaataaaa
    10741 attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt
    10801 ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt tgtggagcgt gaagctttta
    10861 tggtagagga aaatacgaat aaagtgatgt tgataaaaag attcctatct tgaatatgaa
    10921 tcgagatgtc ttgaaaatga atgagtttga ccaatcatta tcctatcgaa atttacagtt
    10981 tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg
    11041 cggcacatct aaatttttaa gtaacggaca gttcaaatat caagcattga aagaggctat
    11101 cctactgttt gaaggaatac gaatatataa aaacgacaaa gaggaaaaag tagttcatta
    11161 aatcccttca agtgatgaaa gaatgagatc taataaaacc tgatctttca tctgtttttt
    11221 taataacgta ataatttttt tattttttgt gctttcgccg ttattgtaga gtgatttagc
    11281 attcttattt ttaaaaataa ctgtgttata tattaaaaaa ggcataatag ttataagctt
    11341 ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat
    11401 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
    11461 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
    11521 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
    11581 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
    11641 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
    11701 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
    11761 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
    11821 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
    11881 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
    11941 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
    12001 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
    12061 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
    12121 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
    12181 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
    12241 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
    12301 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
    12361 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
    12421 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
    12481 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
    12541 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
    12601 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
    12661 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
    12721 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
    12781 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
    12841 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
    12901 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
    12961 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
    13021 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
    13081 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
    13141 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
    13201 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
    13261 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
    13321 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
    13381 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
    13441 aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag ttatgacaca
    13501 agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt gtgagagttt
    13561 gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat gtacctttag
    13621 ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg
    13681 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
    13741 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
    13801 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
    13861 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
    13921 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
    13981 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
    14041 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
    14101 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
    14161 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
    14221 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
    14281 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
    14341 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
    14401 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
    14461 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
    14521 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
    14581 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
    14641 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
    14701 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
    14761 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
    14821 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
    14881 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
    14941 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
    15001 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
    15061 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
    15121 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
    15181 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
    15241 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
    15301 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
    15361 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
    15421 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
    15481 aaaaaatgaa actaacttgc tttgtcatta tgaatttttt caaaaaacat tcttaagaaa
    15541 aaggtggcta tatatagtag tttttggtca atacctttga ataaaaagcc atgaaaaacg
    15601 aatagttagc gcttaccgat gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa
    15661 caaagcatat attttgcttt aaaaccaaat gaggaatatt ctagatgtag aacaggttag
    15721 aaaaagatta gaaaaaggag tgattttgat ggagaagaga tacggcgtag ttcctgtggt
    15781 aactgcttca gacgaaaatt atgctcctta cttgagcgtg atgatcgcaa ctgcattaga
    15841 aaattgcaat aaaacaagac gcataaaatt ttatgtgatt gacgatggtc tatcagaata
    15901 tagtaaagaa ggattggaag aaacggtaaa taaatattca agtaatgcaa gtatccaatt
    15961 cctgacagtg gaaaaagata tctacgaaga cttcctagtc agtgaccata tcacaactac
    16021 cgcttactta agaatttctt taccaaactt attagctaaa gaggattata agaaagtcct
    16081 ttacttagat tcagatgtgt tagtactaga cgatatcgta aaactatatg atgagccgtt
    16141 aaatggaaaa acaatcggcg ctattatcga tccaggccaa gtgaaagctt tggaaagatt
    16201 aggaatcgat tccgatgact tgtacttcaa ttcaggagtg atggtgattg acattgatca
    16261 atggaataaa aaagaaatta cagaaaaaac tatccattac ctcagtgaaa atggcgatcg
    16321 aatcatctat cacgaccaag atgctttgaa tgctgtactt tatgaagatt gggaacaact
    16381 tcatccaaaa tggaacatgc agacttcgct gatttttgaa cgtcatccag caccaaatga
    16441 aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct tcgatcgttc actttacagg
    16501 acatgataaa ccttggaata cgttgaaaga tcatccgtat accaatttat acttaaaaaa
    16561 attagcacac agtactctaa cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag
    16621 tagtcgcaag ttgtaacaca aaattcgtac cacatttagc tgcattattt gtctcagtat
    16681 tagataattg caacccgtct aaatttgttc ggttctatgt gattgacgat gatatcgatt
    16741 ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg
    16801 aattcttgaa aatcaataaa gaattcttta caaatgtggt aatcagcgat cgtattccag
    16861 aaacagctta ttatcgtatt gcgattccgg aattgttccg cggcacagaa gttgaacgta
    16921 tcttatatat ggattgcgac atgatcgctt tacaagatat ctctaaatta tggcgtcttg
    16981 acttcggtga ttcaatcgta gcagctgttg aagacgctgg gttccatcaa cgactggaaa
    17041 aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg
    17101 tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt agatttcatc gaacataatc
    17161 cagaaaaatt acgattccat gaccaagatg cattaaatgc tatcttacat gaccgttggt
    17221 taccacttca tccgcgttgg aatgcacaag gctatatcat ggcaaaagca aaaaaacatc
    17281 caacagcagc aggtgaacgt gaatacgaag aaactcgcaa caatccttac attatccatt
    17341 tttcaggaca tgtgaaaccg tggagcaagg attttgaagg gccaacgaag aaatattacg
    17401 aaaaatatgc agggatgacg gctttccgtt gtgtagctaa attccctaaa tatccaaaat
    17461 atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata atagaagaca acaactgaag
    17521 agggggagta ggaatggaaa ttacaatgtc taaaaatgca gtagagacgt taattgaaaa
    17581 ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat gattccgatc catttctaag
    17641 ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc attccttttg tttcggaatt
    17701 tggagaatat gcgactaaaa tcgagcatcc attattggac atctatacat ctaaattaga
    17761 gcaaaattat ttcggtaaac gattgaacat ggacttcaac aaacagcttg acagcttttc
    17821 attagaaaat gaaatcgcag tactcgatta taatatcaaa ttaaagaact gcttcttcag
    17881 ctaatagttt cgagaaagtc caaaaaggag gtatacattg agatactact ttgtggatga
    17941 agaaaagaat gtaatagaaa cagatgaaaa aaaatataat tggctcgtac ttgatagtgc
    18001 aaagcccgaa gagattgagc aagcaatcga agaattcgag cttcctgatg atattttcgt
    18061 aggaaccgaa tatccggaag aagtttctcg gatggagcgc ctttatgaga cgaaactaga
    18121 gcatccattt tctttggtag tgattaatct agatcagtca gataatcgta tcgaaaaaaa
    18181 attaacgcca atatcttttg tactttccga taatctcttg attacttgta tcgatagaaa
    18241 aaccgaattt gtggatcggc tgctgacaaa gcatggaaat catttaaact cgtttgaaaa
    18301 gatcatcacg tatacattat tagatattta cacccattac gtgaaagaac taagagagat
    18361 gaaaaagcga attgacgctt tggataaagc agctcgtaaa acaacggaaa atgaagaact
    18421 gtataaacag gcagatcttg aaagagacat cgtatatatc gatcatacct taagagatca
    18481 acgggaaaca atggatcaat tatggaattc tgaaactttc atcaaacgtt tagacgatga
    18541 acagctactc tatgatgtcc atttgagaca gcgacaaaca gaaaaaatga tcgagatcta
    18601 tcgagattta ttagagagta ttggtgatct attcaacggg atgatggaca ataatctaaa
    18661 tcatatcatg aaatatctag attcagctgc attaatcatt tccgttccgg cgttgttcgc
    18721 agggatttgg ggaatgaata caggaggatt accgggtaaa agttctgctc taggctttat
    18781 actcgtagtc tttggctctt tgatagcagc agtagtttta ggttaccatt tataccggaa
    18841 agattataca aattaaagaa tctatttaaa acaggtatca aacaaagaaa tttgttttgg
    18901 caatacaatt taaaaactag gaggaataca gcaatgacag aaccaaaatt aatcgacccg
    18961 cgaaacttgt accatacgga gaaatttcct aaacaagatc aggatacacc agctttacag
    19021 cagaatatga ttcctgttcc tgattgtgga gaagaatcct atgaaggcaa taatcaacta
    19081 gaaaaccgcc gtgtactaat cactggtgga gattcaggga tcggtcgtgc agcagcgatt
    19141 gcatttgcta gagaaggggc agatatcgct cttcaattct tcccaggaga agaaaaagat
    19201 gcagaggaag tagcaaaata cgtgaaagtt gcaggacgta agattgtctt actacctttt
    19261 gatcttcgtg acgaacaagc acctaaagaa atcatagata aagccgtgag tgaacttggc
    19321 gggctagata cgatggtact aaacgcagga cagcaaattt cttgtcagtc tattgctgag
    19381 ttaccacttg agcaagtcaa agacacattt atgataaaca tcatcgctat gtttgcttta
    19441 gtgaaagaag ctgttccgca tatcccagca ggaggagcaa tcgtaacaac aacttctgtt
    19501 caagcattca atccaagcga acatttatta gattacgcag caacaaaagc ttcaatcgct
    19561 aactttacga tcggtttagc aaaacagctt gcacctaaag gaatccgtgt aaatggtgta
    19621 gcacctggtc caatctggac accattacaa ctagacgaag gacaaccttc tgattcgatc
    19681 cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc agccaggaga attagcacca
    19741 gtttatgttt tcttagcatc gaataaagca agttatgtta cagcccaaat ttacggagta
    19801 actggtggag aagcgatcaa cttataagaa gttgatcgtt tcacccctat gaggaggtgc
    19861 gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga acattacttt tgctatctgt
    19921 gcttttgttt gcgatactat taagtgcaac ctatccattg ccttttcagc tagagaagtt
    19981 tcgattcttt tccatcacta attcatacgt gcaacaatat gtattttggc ttgccgttgc
    20041 atttgctatt ttgacaatta tcacgttact tgttttcctg ttttatccaa aaacaatcgg
    20101 aaactttcta ctgaaacatg gtgatgggga gttgacgttg gataaaaaag caattgaagg
    20161 aatggttcgt tctcacttgc atgaagatga attcatccat tcaccaaaag taaatgtcaa
    20221 agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact
    20281 cattggtaaa acaggaacat tgatgaaaga aatcgaaaat gaagtacaac acatattggg
    20341 aacaaaagag ccagtgaaag tatcggtgaa atacacaggc tttgaagaac cagctaaacg
    20401 aagcaatgac cgcaaacgag tagaataggg ggggaccaaa aatgaaagaa ctattttcga
    20461 catacaagct ccctatcatc tttggggtac ttggattgat attagccgtt ttatggatca
    20521 ccgtgggctt tttcaagacc ttattgatct tgatcatggc agcgatcggc gtcgctatcg
    20581 gtttttatct gaaacaaacg aaaatcttag atcactattt taaatcttag gaggaaacat
    20641 ccatgaataa caataacaaa aataataaca ataaaatgaa caaccaaaaa ccagtgtctg
    20701 cacatccaga cccaacgaca ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa
    20761 cagcaccagt atctgcacat ccagatccag tagtccctaa cgttcaacaa ggagtagaag
    20821 agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc aggaactcca gaatctaaag
    20881 ttccagaaca acatgatgca atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt
    20941 ctgaccatgc gaaagcacaa gaaaaactta gttctgacca cacaaacgga caaggaaaac
    21001 ttggttctga tcatacaaat aaccaaggaa agcatgattc tgaacaggaa gattcaatgc
    21061 gcggtgagct gaattatgaa gataaagttg tgcaaaaaat catcggaatc gcaattgaac
    21121 aagtagacgg tttattatct gcaaacggtg gtttcttctc taatgtggca ggaaaacttg
    21181 tgaatacaga taatgtaacg gctgggatcg aaacagaagt cggtaaaaaa caagtagcag
    21241 ttgatatgga tgtcattgtg gaatatggca aagatatcga aaaaatcttt gaagaaatgc
    21301 aagaagtaat cggcaaagaa gtaaaaaata tgacacattt agaagtgatc gaagtcaacg
    21361 ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa agatcaagaa acggtgcaag
    21421 acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag
    21481 taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc
    21541 agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg gatttatttg gtcacttatc
    21601 gtcggaggaa ttatcggagc aattgcagga gcaatcacga ataaaggttc ttcgatgggg
    21661 attattgcaa atgtcgtagc aggtttagtt ggatcagcac ttggtcaagc attattaggt
    21721 tcttggggac caagtattgc aggaatggca atcgtgccat cattgattgg tgccatcatt
    21781 ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa aagactttta aaaagaggtc
    21841 gtgaaaaagc tgttcttcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    21901 ttttcatttg gttgggcata ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa
    21961 taagaggctg tgacaagact tttgtcacag cctcttattt atgcgggcaa attcccaatt
    22021 agatttttaa aagtttttcc ttaaatgtgt ctttattgat acagagaagc ttctttaata
    22081 aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg aacatacaaa ataatacgac
    22141 cactttgaaa acccagatga tccacaaatg gatcgtatga tactgatgaa gtaaaaagca
    22201 atggatcaga aactcaaaga gattgaaaaa gacaaggaag aaataatatc tcttttttac
    22261 tcttgtctgt tttagccaat aatagctcgt aaagtaatct agacaaacaa atagaagtaa
    22321 acaaagtttc gtaagagcag caatcgtatc atgttgctct gctagacgat tgacgagcag
    22381 gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa agaaaaaagt aaacaataaa
    22441 tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt
    22501 ttgtgaagct ttttcgtgtc gtaacaaatg ttatctaatg tatttttaaa taaataatca
    22561 ttattatcaa aataatgtta aaaataaaaa agaatt
//
LOCUS       DACDID010000034        22349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDID010000034 DACDID010000000
VERSION     DACDID010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.034"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(342..3995)
                     /gene="rpoC"
                     /locus_tag="IV236_001008"
     CDS             complement(342..3995)
                     /gene="rpoC"
                     /locus_tag="IV236_001008"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8771220.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4074..7691)
                     /gene="rpoB"
                     /locus_tag="IV236_001009"
     CDS             complement(4074..7691)
                     /gene="rpoB"
                     /locus_tag="IV236_001009"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8771221.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8419..9387
                     /locus_tag="IV236_001010"
     CDS             8419..9387
                     /locus_tag="IV236_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8771222.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9405..10310
                     /locus_tag="IV236_001011"
     CDS             9405..10310
                     /locus_tag="IV236_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771223.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10307..11113
                     /locus_tag="IV236_001012"
     CDS             10307..11113
                     /locus_tag="IV236_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8771224.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11270..12238
                     /locus_tag="IV236_001013"
     CDS             11270..12238
                     /locus_tag="IV236_001013"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8771225.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12555..13079)
                     /locus_tag="IV236_001014"
     CDS             complement(12555..13079)
                     /locus_tag="IV236_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8771226.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13366..15177
                     /gene="scm"
                     /locus_tag="IV236_001015"
     CDS             13366..15177
                     /gene="scm"
                     /locus_tag="IV236_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8771227.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15332..16318)
                     /locus_tag="IV236_001016"
     CDS             complement(15332..16318)
                     /locus_tag="IV236_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8771228.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16341..16907)
                     /locus_tag="IV236_001017"
     CDS             complement(16341..16907)
                     /locus_tag="IV236_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8771229.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16921..17337)
                     /locus_tag="IV236_001018"
     CDS             complement(16921..17337)
                     /locus_tag="IV236_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8771230.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17794..19728)
                     /locus_tag="IV236_001019"
     CDS             complement(17794..19728)
                     /locus_tag="IV236_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8771231.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            20030..20965
                     /locus_tag="IV236_001020"
     CDS             20030..20965
                     /locus_tag="IV236_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8771232.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            21055..22158
                     /locus_tag="IV236_001021"
     CDS             21055..22158
                     /locus_tag="IV236_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8771233.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            22171..>22349
                     /locus_tag="IV236_001022"
     CDS             22171..>22349
                     /locus_tag="IV236_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8771234.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT         6606 a         4755 c         4341 g         6647 t
ORIGIN      
        1 aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta
       61 ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc
      121 gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg
      181 attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt
      241 taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga
      301 ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc
      361 attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt
      421 gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg
      481 atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg
      541 atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta
      601 tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg
      661 cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta
      721 cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga
      781 accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt
      841 acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt
      901 ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact
      961 ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt
     1021 acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct
     1081 gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga
     1141 ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga
     1201 gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg
     1261 tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta
     1321 catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg
     1381 atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt
     1441 acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt
     1501 ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg
     1561 attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc
     1621 aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg
     1681 gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg
     1741 gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca
     1801 gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg
     1861 atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg
     1921 atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg
     1981 atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt
     2041 gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact
     2101 ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat
     2161 actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg
     2221 tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga
     2281 ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc
     2341 cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa
     2401 tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct
     2461 tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga
     2521 gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc
     2581 aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct
     2641 ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct
     2701 tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc
     2761 agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga
     2821 cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca
     2881 cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac
     2941 atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct
     3001 agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat
     3061 gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct
     3121 actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct
     3181 aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca
     3241 cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca
     3301 gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa
     3361 atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct
     3421 ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct
     3481 gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt
     3541 tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa
     3601 gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt
     3661 gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc
     3721 atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt
     3781 cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca
     3841 aaaatccgct cacagaacaa cccttcacgt tcaggtttta aggtacgata gttaatcgtc
     3901 tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct
     3961 atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa
     4021 tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct
     4081 aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt
     4141 tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca
     4201 tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct
     4261 tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg
     4321 aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt
     4381 ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc
     4441 gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga
     4501 gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc
     4561 aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt
     4621 ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata
     4681 ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt
     4741 gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg
     4801 atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca
     4861 ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga
     4921 cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact
     4981 aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg
     5041 tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct
     5101 ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt
     5161 ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata
     5221 cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt
     5281 tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa
     5341 gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac
     5401 ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc
     5461 atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt
     5521 ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta
     5581 tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca
     5641 ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca
     5701 gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca
     5761 cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg
     5821 gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag
     5881 ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat
     5941 gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt
     6001 tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg
     6061 aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca
     6121 ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa
     6181 ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc
     6241 aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat
     6301 tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca
     6361 gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg
     6421 aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata
     6481 tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg
     6541 tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact
     6601 tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg
     6661 accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga
     6721 gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct
     6781 gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct
     6841 aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag
     6901 aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa
     6961 cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag
     7021 tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca
     7081 tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg
     7141 atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta
     7201 gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag
     7261 acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat
     7321 gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg
     7381 ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca
     7441 tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc
     7501 aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt
     7561 ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc
     7621 aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg
     7681 tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg
     7741 ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag
     7801 gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt
     7861 tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga
     7921 tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt
     7981 ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg
     8041 acaattctta attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg
     8101 aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta
     8161 ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt
     8221 ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc
     8281 taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt
     8341 attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa
     8401 attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta
     8461 ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa
     8521 acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc
     8581 aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa
     8641 aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat
     8701 cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc
     8761 actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc
     8821 aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa
     8881 atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac
     8941 ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc
     9001 gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt
     9061 ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca
     9121 agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat
     9181 caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa
     9241 acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa
     9301 acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat
     9361 ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct
     9421 ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat
     9481 acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag
     9541 gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc
     9601 taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga
     9661 aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc
     9721 gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg
     9781 agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga
     9841 tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa
     9901 ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca
     9961 tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca
    10021 acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta
    10081 tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt
    10141 taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa
    10201 gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc
    10261 agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt
    10321 aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct
    10381 gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg
    10441 ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca
    10501 aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat
    10561 cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa
    10621 tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca
    10681 agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt
    10741 atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc
    10801 cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc
    10861 tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc
    10921 ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct
    10981 gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc
    11041 ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact
    11101 tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt
    11161 tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt
    11221 tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa
    11281 agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc
    11341 acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc
    11401 aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga
    11461 tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat
    11521 gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt
    11581 ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt
    11641 tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt
    11701 tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc
    11761 tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa
    11821 agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat
    11881 catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca
    11941 agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat
    12001 cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt
    12061 ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac
    12121 ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga
    12181 acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt
    12241 atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc
    12301 aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct
    12361 aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga
    12421 gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat
    12481 agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt
    12541 ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca
    12601 gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt
    12661 gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa
    12721 atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg
    12781 atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc
    12841 ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca
    12901 atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc
    12961 ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa
    13021 cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt
    13081 tattttcctc ctttttattt atcctactta ctttttccta attctaggat acctacttat
    13141 atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag
    13201 attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt
    13261 tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa
    13321 aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca
    13381 agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca
    13441 gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac tggtaactat
    13501 ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat
    13561 gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca
    13621 gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc
    13681 gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt
    13741 aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac
    13801 ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag
    13861 gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca
    13921 tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca
    13981 gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt
    14041 caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca
    14101 ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt
    14161 tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct
    14221 ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac
    14281 ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac
    14341 gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca
    14401 tctaccacaa gtgatagctc agattcatca acttcaagcg acagtacaga tactacctct
    14461 accacaagtg atagcacgga tacatcagct tcaagcgaca gtacagatac tacctctacc
    14521 acaagtgata gcacggacac atcagcttca agtgacagta cagatactac ctctaccaca
    14581 agtgacagca cggacacgtc agcttcaagc gacagtacag atactacctc taccacaagt
    14641 gatagcacgg acacatcagc ttcaagtgac agtacagata ctacctctac cacaagtgat
    14701 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
    14761 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
    14821 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
    14881 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
    14941 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
    15001 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
    15061 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
    15121 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
    15181 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
    15241 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
    15301 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
    15361 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
    15421 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
    15481 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
    15541 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
    15601 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
    15661 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
    15721 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
    15781 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
    15841 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
    15901 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
    15961 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
    16021 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
    16081 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
    16141 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
    16201 tgatggcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
    16261 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
    16321 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
    16381 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
    16441 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
    16501 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
    16561 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
    16621 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
    16681 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
    16741 tgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
    16801 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
    16861 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
    16921 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
    16981 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
    17041 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
    17101 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
    17161 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
    17221 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
    17281 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
    17341 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
    17401 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
    17461 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
    17521 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
    17581 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
    17641 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
    17701 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
    17761 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
    17821 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
    17881 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
    17941 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
    18001 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
    18061 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
    18121 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
    18181 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
    18241 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
    18301 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
    18361 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
    18421 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
    18481 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
    18541 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
    18601 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
    18661 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
    18721 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
    18781 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
    18841 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
    18901 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
    18961 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
    19021 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
    19081 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
    19141 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
    19201 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
    19261 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
    19321 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
    19381 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
    19441 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
    19501 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
    19561 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
    19621 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
    19681 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
    19741 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
    19801 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
    19861 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
    19921 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
    19981 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
    20041 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
    20101 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
    20161 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
    20221 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
    20281 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
    20341 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
    20401 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
    20461 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
    20521 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
    20581 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
    20641 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
    20701 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
    20761 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
    20821 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
    20881 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
    20941 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
    21001 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
    21061 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
    21121 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
    21181 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
    21241 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
    21301 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
    21361 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
    21421 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
    21481 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
    21541 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
    21601 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
    21661 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
    21721 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
    21781 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
    21841 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
    21901 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
    21961 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
    22021 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
    22081 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
    22141 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
    22201 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
    22261 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
    22321 tttgatttag cgactaccgc tatcaataa
//
LOCUS       DACDID010000035        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDID010000035 DACDID010000000
VERSION     DACDID010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.035"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV236_001023"
     CDS             <1..395
                     /locus_tag="IV236_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771235.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IV236_001024"
     CDS             398..1147
                     /locus_tag="IV236_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8771236.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IV236_001025"
     CDS             1159..1428
                     /locus_tag="IV236_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771237.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IV236_001026"
     CDS             1590..1892
                     /locus_tag="IV236_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771238.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IV236_001027"
     CDS             1889..2050
                     /locus_tag="IV236_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8771239.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IV236_001028"
     CDS             2047..2352
                     /locus_tag="IV236_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771240.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IV236_001029"
     CDS             2352..2708
                     /locus_tag="IV236_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8771241.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IV236_001030"
     CDS             2668..2913
                     /locus_tag="IV236_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771242.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IV236_001031"
     CDS             2910..3329
                     /locus_tag="IV236_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8771243.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IV236_001032"
     CDS             3326..3883
                     /locus_tag="IV236_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8771244.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IV236_001033"
     CDS             3880..4176
                     /locus_tag="IV236_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771245.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IV236_001034"
     CDS             4253..4666
                     /locus_tag="IV236_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8771246.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IV236_001035"
     CDS             5012..5386
                     /locus_tag="IV236_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771247.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IV236_001036"
     CDS             5616..5960
                     /locus_tag="IV236_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8771248.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IV236_001037"
     CDS             5965..6246
                     /locus_tag="IV236_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771249.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IV236_001038"
                     /pseudo
     CDS             6349..6663
                     /locus_tag="IV236_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
     gene            6641..8335
                     /locus_tag="IV236_001039"
     CDS             6641..8335
                     /locus_tag="IV236_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8771250.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IV236_001040"
     CDS             8355..9533
                     /locus_tag="IV236_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8771251.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IV236_001041"
     CDS             9496..10182
                     /locus_tag="IV236_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8771252.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IV236_001042"
     CDS             10182..11342
                     /locus_tag="IV236_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8771253.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IV236_001043"
     CDS             11352..12227
                     /locus_tag="IV236_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771254.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IV236_001044"
     CDS             12224..12535
                     /locus_tag="IV236_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771255.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IV236_001045"
     CDS             12525..12878
                     /locus_tag="IV236_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8771256.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IV236_001046"
     CDS             12868..13269
                     /locus_tag="IV236_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771257.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IV236_001047"
     CDS             13262..13666
                     /locus_tag="IV236_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771258.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IV236_001048"
     CDS             13678..14286
                     /locus_tag="IV236_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8771259.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IV236_001049"
     CDS             14306..14668
                     /locus_tag="IV236_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771260.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IV236_001050"
     CDS             14671..14853
                     /locus_tag="IV236_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771261.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IV236_001051"
     CDS             14870..18289
                     /locus_tag="IV236_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8771262.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IV236_001052"
     CDS             18340..19077
                     /locus_tag="IV236_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8771263.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IV236_001053"
     CDS             19087..21378
                     /locus_tag="IV236_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771264.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IV236_001054"
     CDS             21402..>21988
                     /locus_tag="IV236_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771265.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7934 a         3245 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta aagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACDID010000036        20915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDID010000036 DACDID010000000
VERSION     DACDID010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.036"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..1385
                     /gene="rseP"
                     /locus_tag="IV236_001055"
     CDS             117..1385
                     /gene="rseP"
                     /locus_tag="IV236_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8771266.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1453..3162
                     /locus_tag="IV236_001056"
     CDS             1453..3162
                     /locus_tag="IV236_001056"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8771267.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3374..7732
                     /locus_tag="IV236_001057"
     CDS             3374..7732
                     /locus_tag="IV236_001057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8771268.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7877..8350
                     /gene="rimP"
                     /locus_tag="IV236_001058"
     CDS             7877..8350
                     /gene="rimP"
                     /locus_tag="IV236_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8771269.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8373..9548
                     /gene="nusA"
                     /locus_tag="IV236_001059"
     CDS             8373..9548
                     /gene="nusA"
                     /locus_tag="IV236_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8771270.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9570..9863
                     /locus_tag="IV236_001060"
     CDS             9570..9863
                     /locus_tag="IV236_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8771271.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9860..10171
                     /locus_tag="IV236_001061"
     CDS             9860..10171
                     /locus_tag="IV236_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8771272.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10184..12490
                     /gene="infB"
                     /locus_tag="IV236_001062"
     CDS             10184..12490
                     /gene="infB"
                     /locus_tag="IV236_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8771273.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12517..12864
                     /gene="rbfA"
                     /locus_tag="IV236_001063"
     CDS             12517..12864
                     /gene="rbfA"
                     /locus_tag="IV236_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8771274.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13021..13194)
                     /locus_tag="IV236_001064"
     CDS             complement(13021..13194)
                     /locus_tag="IV236_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771275.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13642..16004
                     /locus_tag="IV236_001065"
                     /pseudo
     CDS             13642..16004
                     /locus_tag="IV236_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16372..17295
                     /gene="truB"
                     /locus_tag="IV236_001066"
     CDS             16372..17295
                     /gene="truB"
                     /locus_tag="IV236_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8771276.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17299..18240
                     /gene="ribF"
                     /locus_tag="IV236_001067"
     CDS             17299..18240
                     /gene="ribF"
                     /locus_tag="IV236_001067"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8771277.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18592..19377
                     /locus_tag="IV236_001068"
     CDS             18592..19377
                     /locus_tag="IV236_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8771278.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19390..20241
                     /locus_tag="IV236_001069"
     CDS             19390..20241
                     /locus_tag="IV236_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8771279.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20365..20817)
                     /locus_tag="IV236_001070"
     CDS             complement(20365..20817)
                     /locus_tag="IV236_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8771280.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7157 a         3678 c         4562 g         5518 t
ORIGIN      
        1 tttttatttt tcattatatt gatactattg gtgtctttgg tatactatgg taaaattata
       61 gtgttttatc gttgttttat aaaaaacaca aaaaacaagt aaaggagaaa aaagctatga
      121 aaacgattct gacatttatc atcgtttttg gtatattagt gattgttcat gagtttggtc
      181 atttcttctt tgcgaaacga tcaggaatcc tcgttcgcga atttgctatc ggtatgggtc
      241 cgaaaattta tgggcatcaa gcaaaagacg ggacaacata tacgcttcgc ctattaccta
      301 ttggcggtta tgttcgaatg gcaggcaatg gggatgatga aacagaaatg gcaccaggaa
      361 tgcctctttc tcttttacta aattctgacg gcatcgtaga gaagataaat ttaagtaaga
      421 aaatccaatt gacaaatgct atcccgatgg aattgagccg ttatgattta gaagatgagc
      481 tgactatcac tggttatgtg aatggagacg aaacagaagt agtaacctac cctgttgatc
      541 acgatgcgac gattattgaa aacgacggaa cagaaatcag aattgcgcca aaagacgtac
      601 agttccaatc ggctaaattg tggcagcgta tgctgaccaa ctttgctgga cccatgaata
      661 actttatttt ggcaatcctg ttattcatca ttctggcatt tatgcaaggt ggtgttcaag
      721 tgacgaacac taatcgcgta ggagagatca tgcctaatgg agctgcggca gaagcaggct
      781 taaaagaaaa tgatgaggta gtcagtgtgg acggaaaaga aatccattca tggaatgatc
      841 tgacaacagt cattacgaaa aatccggata aaacattaga ctttaagatc gaacgagaag
      901 gtcaggtgca atctgtcgac gtgacaccga aatctgtaga gtcaaatggc gaaaaagttg
      961 gccaacttgg tataaaagcc ccaatgaata caggttttat ggataagatc attggaggga
     1021 cgcgtcaagc tttcagcggt tcattggaaa ttttcaaagc cttaggatca ctatttacag
     1081 gtttcagttt agataaatta ggcggacctg tcatgatgta ccaattgtct tcagaagcag
     1141 caaatcaagg tgtaacaact gttattagtt tgatggcgct tctttcaatg aatctcggaa
     1201 tcgtcaatct gcttccgatt cctgccttag atggcgggaa attagtcttg aatatttttg
     1261 aagggattcg cgggaaacca ttaagtcaag aaaaagaagg aatattgaca ttagctggct
     1321 ttggattttt aatgttgttg atggtgctag ttacatggaa cgatattcaa cgattctttt
     1381 tctagaaaaa gttgacaaat agggtataat aacaaacgat aactattttg aggaaataaa
     1441 ggagtatata gaatgagaca atcaaaaatg ttgattccaa cattacgcga agttccaaat
     1501 gatgcagaag tattaagcca tcaaatcttg ctaagagctg gatatatccg ccaagtatca
     1561 gcgggtattt attcatatct gccattagca aatcgtgtat tggaaaaact gaaacgcatt
     1621 atgcgggaag aatttgaaaa aatcggagcg gtagaaatgc tgatgcctgc catcttgcca
     1681 gcagaacttt gggaagaatc cgggcgttac gaaacatatg gaccaaactt gtatcgtttc
     1741 aaagaccgca atgatcgaaa aatgatcctt ggaccaactc acgaagaaac ttttactgat
     1801 ttgatgcgta atgaaatcaa ttcatataag aaattacctt taaatcttta tcaaatccaa
     1861 cctaaatatc gtgacgaaaa acgacctcgc ttcggtttgc ttcgcggaag agagtttatc
     1921 atgaaagatg cgtattcttt ccatgcaacg gaagaaagtt tggacgagac atataaagat
     1981 tacgaaaaag cctacaccga agtattcaag cgttgtggct tagaatttcg ctcgattatc
     2041 ggagacggtg gcgcaatggg cgggaaagac tcaaaagaat tcatggcgat ctcagaaatc
     2101 ggggaggata cgatctgtta ttcgactgaa agtgattatg ctgcaaactt ggagatggct
     2161 acaagctact atgtatcaaa aaaatcgcat gaaacacagc ttgaattgga gaaagtagct
     2221 acacctaacg taaaatcaat tgacgaagta gcagatttct tttctgttga acctcaaaaa
     2281 atcatcaagt ctgtattgtt cattgcggat gaacaacctg tccttgtttt ggtacgcgga
     2341 gaccatgaag tcaatgacgt aaaactgaaa aactttttat ctgctgactt tttagaagaa
     2401 gcaacagaag aagaagcaca aaaatatcta ggtgctgagt ttggttctgt cggaccagtc
     2461 ggtgtttcag atgacgtaaa agtatatgca gaccgttatg tccaagattt agcgaatgcc
     2521 attactggag cgaacgaaac aggattccac tataaaaatg tgaatccaga acgcgatttc
     2581 actgtacttt cttatgaaga tcttcgtttt gtacaagaag gagatccttc accagacaac
     2641 aatggtgttc ttgcctttac tcgaggaatc gaaatcggac atatcttcaa aatcggcact
     2701 cgttacagtg aatctatggg agcaactgta ctagacgaaa atggacgaga aaaatttgtg
     2761 attatgggct gttatggtat cggtgtaagt cgtttattat cagcaatcgt agaacaacat
     2821 tcagatgaac aaggaatcaa ctggccaaaa ggaattgcgc catttgattt gcatatcgtc
     2881 caaatgaatc taaaagacga ataccaaaca aacctgacga atgaactaga agaagcgatg
     2941 acaaaagctg gctatgaagt actggttgat gacagaaatg aacgcgcagg agtgaaattc
     3001 gcggattctg atttgattgg ctgtccaatc agaatcactg tcggtaaaaa agcagtggat
     3061 ggaatcgtag aagtgaaaat caagaaaact ggcgaaatgg ttgaagtacg taaagaagaa
     3121 ttggtgaata ctttaccaat cctcttgaac caaggcgaat aataagcgtt aaaacacaga
     3181 aaataaaaat agctgaatca gctgtttttg acaatctgat gattcgcttt taaaattgga
     3241 actgcgattt gaatcgcagt tccaattttt taatgtaaaa ctcagaaaaa acgagtatac
     3301 taaaaggtac tgaaaaagaa gaggataaga tgcgatcaat tgcattgttt ctcatcagaa
     3361 agggcgtata tctttgtcag aaaaacgaga tttatttgaa aaactgttgg aacaaatcca
     3421 actagaggaa gcagaaaaaa atcatccact gcttactgct ggtgaaatgg atagtgtagt
     3481 ggtccatcgt aaatcgcgtt tatgggaatt tacgctgcat ttttccaaaa ttttgcctat
     3541 tatgctatat cggtcattga ctcagcatct gactatcgct tttaaagata ttgcacaagt
     3601 caaactgaat atcttagcag atgaccagac attcgatgaa cagctcttgc aagattactg
     3661 ggcccaagct ttggaaaatc agcaatgcga tacaccacta gcacagcaag tattgaaaac
     3721 gcaggtacct gtgataaaag atcgtaaagt catcttgcca atcgacagta caggagctat
     3781 ttcttatttg aagcaacaat acttgccatt agtagaagag ttgttcgtca gttacggatt
     3841 cccaaaattt cgtatcgaac cagaagtgga tgagcagcaa gcggaacgag tattgaaact
     3901 ttttgaagaa cgcaaacaag aacaggcaga agcctttatg aagcaagcag ctgaaagtct
     3961 agttgtccac gaacaaaaga aaaaggaaag aaaagagcaa tcgccagctc ttgaaggacc
     4021 catccacatc cagctaggac gaaatattcc agccgatgaa ccaacgacac caatgatcag
     4081 tatcgttgaa gaagaacgaa gagtcacact tgaggggtac gtattcgaca aagaagtcag
     4141 agaactacgt tcaaaacgca agatcttgac tttgaaaatc acagattata cttcatcatt
     4201 tatcgtcaaa aaattctcga acggcgaaaa agatgaacaa gtatttgatg cgatcagtgt
     4261 cggtagctgg ttaaaggtac gaggaagtat tcaagaagat acgtttgtcc gtgatttggt
     4321 catgaatgcg caagatatca tagaagtcaa acatacacct cgcaaagatt atgctcctga
     4381 aggggaaaaa cgagtagagc ttcatgttca tagcaatatg agtacgatgg atgcaacaaa
     4441 cagcatctct gatcttgtgg cacaagcagg gaaatgggga caccgagcta tcgccattac
     4501 agaccacggt ggtgcgcaag cctttccgga agcacatagt gctggaaaaa aagcaggtgt
     4561 gaaaatctta tacggcgtgg aagcgaatgt cgtagatgat ggtgtcccaa ttgcttataa
     4621 tgatgcacat gaagcattga gtgaagccac atatgtcgta tttgacgtgg aaacgacagg
     4681 attgtctgca gtatatgaca cgatcatcga gctagccgca gtcaaaatgt ataaaggaaa
     4741 tgtgatcgaa agctttgatg aatttatcga tccaggacat cctttgtcac gaacaacggt
     4801 agatctgact ggtatcacgg atggaatggt tcgaggatca aagtcggaag aagaagtatt
     4861 gcggatgttc cttgaatttt caaaagatac aattttggta gcccacaacg cggcttttga
     4921 tatggggttc ttgaatacta gctacgcaag gtatggtatt cctgaagcgg ccaatccagt
     4981 cattgatacg ttggaattag ctagatatct gtatcctcaa ttcaaacgtt tcggattagg
     5041 tgtgttatct aaaaaatttg gtgtcagctt ggaacagcat caccgagcaa tctatgatgc
     5101 tgaagcaact ggtcacttag catggatctt tgtgaaagag gcaatggaca atcataacat
     5161 gctttatcat gatcagctaa atgagcatat cggagaagga gattcttaca aaagggctcg
     5221 acctttccat gtaacgattt tagcaaaaaa tcaggcagga ctgaaagatt tgttcaaact
     5281 gatttcaatg tcgaatgtcg aatacttcga acgcgtccca cgaataccac gctcccaatt
     5341 gaaaaagatg cgtgaaaatc tattgatcgg ctcagcttgt gataagggag aaatattcga
     5401 agccatgatg caaaaagggg tagaagaggc aagaaaccga gcaaaattct acgattatat
     5461 tgaagttatg ccaaaagcgg tctatgcgcc tttgatcgag caagaattgg taaaaaatga
     5521 acatgatttg gaagaaatta tccaaaattt ggtagaaatc ggaaaaagtt tggataaaat
     5581 cgttgttgca acaggtaatg tacattatct gaacgaagaa gatgccattt accgaaaaat
     5641 tttgatcaat tctatgggcg gagcgaatcc gttgaatcgt catagtttgc cggatgtcca
     5701 tttccgaaca accgatgaga tgctaactgc atttcacttt ttaggggaag aaacagcgaa
     5761 agagatcgta gtagaaaata cgaataaaat cgcagatatc tgcgaagaag tgattccagt
     5821 caaagatgaa ctttatacac cgaagattcc aggttcagaa gatgaaatat cagaattgag
     5881 ttacacaaaa gcaaaacaaa tgtacggaga tccgttacct gagatcattc aaaaacgatt
     5941 aaaaaaagag ttgaattcta ttaatggtaa tggcttctcg gttatttatc tgatcgccca
     6001 aaaactagtg cataaaagta atgaagatgg ctatctagta ggttcacgtg gctctgttgg
     6061 ctctagcttt gttgcgacga tgacaggaat cactgaagtt aatccgttag cccctcatta
     6121 ttattgtcca gagtgccaat attccgagtt ttttgaagat ggtacgtatg gttctggatt
     6181 tgacatgcca gaaaaacaat gtccaaaatg tggtgcacgc ttaaacaaag acggtcatga
     6241 tattccattt gagacatttt taggattcca tggagacaaa gtgcccgata ttgatttgaa
     6301 cttctcaggt gattatcaag cagaagccca taactatacg aaagtattgt ttggcgaaga
     6361 ctatgtttac cgtgccggaa cgattggtac agtcgccgac aaaacagcat atggctatgt
     6421 aaaaggatac gaaagggaca ataatctgca gtttcgaagt gcagaagtcg acagattagc
     6481 aaaaggcgct actggagtca aacggacaac cggccagcat ccagggggga tcatcgttat
     6541 tcccgattat atggatgtct atgattttac gccgatccaa tatccagcag acgatcaaaa
     6601 ttcggaatgg aaaacgactc attttgattt tcactcgatt cacgataatg tattgaaatt
     6661 agatattcta gggcatgatg acccaactgt tattcggatg cttcaagatt tatcagggat
     6721 cgatccacaa acgatcccta cggatgaccc ggaagtgatg cggatatttg ccggtcctga
     6781 agtacttgga gtaagtcagg aacaaattta ttccaaaaca gggacattag gtattccaga
     6841 attcggcact cgttttgttc gagggatgct agaagaaacg catcctacaa cttttgcaga
     6901 actactgcaa atttccggac tatctcatgg tacagacgta tggttaggaa atgcggaaga
     6961 attgattcga cgaggagatg cgacactggc ggaagtaatc ggctgtcgtg atgatattat
     7021 ggtttatttg atccatgctg gattagatag cgggatggca tttaaaatca tggaaactgt
     7081 tcgtaaagga caatggaaca agatccctga tgaattgcga gaaacctatc tatcggcgat
     7141 gaaagaaaat aatgtgcccg actggtatat cgattcttgt tcaaaaatca aatatatgtt
     7201 tccgaaagcc catgctgcag cttacgtatt gatggctctt cgtgtcgctt atttcaaggt
     7261 ttatttccct attttgtatt actgtgcata cttttctgta cgtgcagacg actttgatct
     7321 agtctccatg tgcaaaggaa aagatgctgt gaaacaagca atgaaagaaa tcacggacaa
     7381 aggattagat gcttccgtaa aagaaaagaa tcaactgaca gtgctggagt tagccaatga
     7441 aatgctggaa cgaggattca aatttggaat gattgatttg tataaatcgg atgccgttaa
     7501 ctttgttatt gaaggtgata cgttgatcgc accatttaga gctgtcccaa gtttgggaac
     7561 caacgtggca aaacaaatcg ttgaagcaag aaaagacggt ccatttttat ccaaagaaga
     7621 tcttgccacc cgcggtaaag tttcaaaaac attgatcgaa tatatgaacg acaatggcgt
     7681 attgaaagat ctaccagatg aaaatcaatt atcgttattc gatatgctat aagcatctgt
     7741 tttcctgtct gcttgtaatt ttaagcagag tatggtatag ttcttatagg taatgatact
     7801 aactgtgagt gagcggattt ttcgctcact ctttttaatg gaattacgca cttaattttt
     7861 taaggaggcg aaacttttga gtagcgtcgt tgaaacagtc acagaaatgg tgacaccgat
     7921 tttagatgaa caaaattttg aacttgtcga agtcgaattt gtaaaagaag gaaaaagctg
     7981 gttcttacga gtatttattg acaaagaagg cggtatcgat atcgaagaat gcgcctttgt
     8041 cagtgagaaa ctgagtgaaa aacttgatgc agcagatcca gatccgattc ctcaagctta
     8101 tttcctggag gtatcctccc cgggagcaga gcggccattg aaaaaagaaa gcgactatga
     8161 aaaggcactt ggtgattatg tccatgtctc cttataccaa ccagtagatg gcgaaaaaca
     8221 atatgaagga tttcttcaat catttgatgc tgaacagctt acattgaaaa tacgcataaa
     8281 aacacgagaa aaagaattga cttttgaccg aaaaaatatt gcaaaagctc gcttagctat
     8341 ccaattttaa tcacggagag gaatcagaaa aaatgagtaa agaaatgtta aatgcgttag
     8401 acgctttaga agctgaaaaa gggatctcaa aagaaatcgt gatcgatgct ttagaagcag
     8461 cattggtttc tgcatacaaa cgtcactatg gccaagcaca aaacgtcgaa gtggaatttg
     8521 atcagaaaaa aggaaagatc catgtctatg cggtaaaaga agtaacggaa gaagtgatgg
     8581 attcacagtt ggaagtctca ttgaaagatg cacttttaat caatccagca tatgagatcg
     8641 gtgatacgat ccgttttgaa gtaacaccaa aagattttgg gcggatcgct gcgcaaacgg
     8701 ctaaacaagt tattttacag cgtgtacgtg aagcagaaag aacgattatt tataacgaat
     8761 tcagtgctta tgaaaaagac atcatgcaag gaatcgtcga acgccaggat aaacgttata
     8821 tttatgtcaa tcttggtaaa attgaggcag ttctttctaa acaagatcaa atgccaaatg
     8881 aattttatca accacatgat cgaatcaaag tttatgtttc ccgagtagaa aatacgtcta
     8941 aaggtccgca agtatttgtt agccgcagcc atccagacct attgcgccgc ttatttgagc
     9001 aagaagtacc agaagtctat gatggaatcg ttgaaattgt cagcatcgcc cgtgaagctg
     9061 gcgatcgttc aaaagtggca gttcgctcaa atgatccaaa tatcgatgca gtcggtacat
     9121 gtgtcgggcc aaagggacaa cgagttcaag caatcgtcaa tgaattaaaa ggcgaaaaca
     9181 tggatatcgt cgaatggaat gaagatccag ctgtattcat tgcaaatgca ttgaatcctg
     9241 cacaagtcat ggatgtgatc ttcgatgaga caaatccaaa ggcctgcaca gttgtcgttc
     9301 cagactacca attatcgcta gcaatcggta aacgcggaca aaacgcacgt ttagctgcta
     9361 aattgactaa ccataaaatc gatatcaaat cagaatctga catgacggaa ttttatgaaa
     9421 atcaagaaca acaaaaagaa acagaagaac tgcatgatga agcaatcgtt cagtcagatt
     9481 taacagacga tgaatatgaa acaattgcat tcaacaatga aacggtcgaa gaaaaaccag
     9541 aagcttaact ttagtaagga ggcaaagaga tgaaacaaag gaaaattcct ttacgcaaat
     9601 ccgtggtttc aggtgaaatg aaacctaaaa aagagatggt ccgaatcacc cgttcaaaag
     9661 aaggggaagt atcgatcgat ccaactggaa aaatgcctgg acgaggagcg tatgtctcac
     9721 ttgagccgga agaagtacaa caagcttggg ataaaaaaat tctcgatcga gtactcgaaa
     9781 caacattaac agatgagttt tatcaagaac ttcttgacta tgtcactcac caaaaagcac
     9841 gtaaagagtt atttggaaaa tgaatacagt taatcggcaa aaagccatga atctcatcgg
     9901 tctagcgatg cgcgcgggaa aactaatcac gggtgaagaa ctgaccattg ctgaaatccg
     9961 caaacaaaaa gcaaaattgg tgtttgtggc aaccgatgca agcgagaata caagaaaaaa
    10021 gataaaagat aagagttcat attatgaagt tccttgcttt gagctatttt ctgaagcaga
    10081 aatcacgcag atgatcggaa aacctcgcaa ggtattcgga ataatggatg ccggatttgc
    10141 gaagaagacc aaggagctaa ttgaaggtta ggaaggtgat tgcatgggca acaaaagaat
    10201 ctatgaatta gcaaaagaac tcaaaaaatc tagtaaagac gtcgtggaaa aagcacaaca
    10261 acttggcatc gatgtgaaaa accatatggg tgcgattact agcaatgatg agaagaaatt
    10321 gcaacaagcg ttcaaaaacc caagcacgaa acaacaagct gcacaacaag caagcagaaa
    10381 accagccaat caacagccga acgatcaacg taaaaaagaa acaacgaaca atcaacaaaa
    10441 aaataaaaat aaccgcagtt atcaagatcg cggtcaaggg agcggtcaag tgaatcaagg
    10501 aaaaaaacca acaacaaacc aaaataatac gggaaatacc agcagccaaa atcgtcaagg
    10561 aacgagccaa ggcggcaatc aaaaccgtac taacaataac aacaaaccaa acaataacaa
    10621 tcgcggcaaa tttaataata ataaccgtaa tcgttataat aaaaaaggta aaaaaggcaa
    10681 acagcaacaa tcaactaaac cagctgtacc accacgcaaa ttccgtgaat tgccagatgt
    10741 attagagtat actgaaggta tgaacgtagc agatattgcg aaaaaaattc atcgtgagcc
    10801 agcagaaatc attaaaaaat tatttatgct tggtgtcatg gtcaatcaaa accaagcact
    10861 ggataaagat acgattgaac tgttagcgac ggactatggc atggaaccac aagaaaaagt
    10921 ccaagttgac attgctgata tcgataaatt cttcgaggca gaagaagtcg atccagataa
    10981 attagtctca cgtccgccag tcgtaacgat tatgggacac gtagaccatg ggaaaacaac
    11041 tttattggat acattacgcc attcacgtgt aacaagcggc gaagccggcg gaatcacaca
    11101 gcatatcggt gcctaccaga tcgatattga tggtaaacca atcactttct tggatacacc
    11161 aggacatgcg gcctttacaa gtatgcgtgc acgtggagca agtatcacag atatcacgat
    11221 cttagttgtt gctgcagatg atggcgtcat gccacaaaca gttgaagcaa tcaaccatgc
    11281 caaagctgca ggcgttccaa tcatcgtcgc tgtaaataaa attgataaac caggtgcaaa
    11341 ccctcaacat gttatgcaag aattaagtga gtatgagtta attcctgaag catggggtgg
    11401 agaaacgatc tttgttgaga tctcagcgaa attcggccaa aacatcgaag aattgctgga
    11461 aatgatcctg ttagtcgcag aagtagaaga tttaaaagca gatccaacac aacgcgcaat
    11521 cggaacagtg atcgaagcac gtcttgataa aggtaaaggt cctgtgtcaa ccttacttgt
    11581 ccaagaagga acacttcatg ttggtgaccc aatcgttgtc ggtaatactt acggacgtgt
    11641 ccgtgtaatg acaaacgatc ttggccgtcg cgataaagaa gcaggacctg caacgccagt
    11701 agaaatcact ggcttgaatg atgtacctca agcaggtgac cgtttcgtag tcttcgaaga
    11761 cgaaaagaca gcacgtgcag cgggtgaaga acgcgcgaaa cgtgcattat tagaaaaccg
    11821 tgctgccaca agccgtgtga ctttggataa cttatttgaa agcctgaaag aaggcgaatt
    11881 gaaagaagtt aacgtaatca tcaaagcaga tgtacaaggt tcagcagaag cactggctgc
    11941 atcattgaag aagattgatg tggaaggtgt ccgtgtaaaa atcgtccatt cagctgtcgg
    12001 agcaatcaat gaaagtgatg taaccttagc agcagccagc aacgctatta tcatcggttt
    12061 caacgttcgt cctacaccgc aggcaaaagt gcaagctgat actgaagaag tcgatattcg
    12121 tcttcaccgt atcatctata aagccatcga agaaatcgaa actgcgatga aagggatgct
    12181 agaccctgaa tttgaagaaa aaatcaccgg tcaaatgatc gttcgtgaaa ccttcaaagt
    12241 ttctaaagtt ggtacgattg caggtgccta tgtaacagaa ggatatattc gtagagatag
    12301 cggtgtccgt gtaatccgtg acggaatcgt aatctatgaa ggccaacttg caagtctgaa
    12361 acgattcaaa gatgacgtga aagaagtcaa aatgggttat gaatgtggtg caatgattga
    12421 aaaattcaat gacatcaaag tagatgacgt aattgaagga tttatcatgg aagaagtcaa
    12481 aaatgactaa aaactgataa aaggaggcca atcattatgg ctaactaccg tgaccgccga
    12541 gtcggtcaag aaatcttaaa agaagtcaat gatattttgc gcaaaaaggt ccgtgaccct
    12601 cgtgtggaga acgtaacgat cacagatgtg aaagttacgg gggacttaca gcaagcaacg
    12661 atctattaca gcattttatc tgacttagca tcagataaga aaacagcaca agatggattg
    12721 aataaagcat caggattgat tcgtcgagag ttagggcaca atctaagtat ttataagaca
    12781 cctgaattgt tctttgaact tgatgaatca gttgtctatg gcaaccatat cgatgaattg
    12841 cttcgcgact tgaacaaaga ctaatcaaaa actcccgcaa atcctgtgaa atcaggggtt
    12901 gcgggatttt tttgttttca ttaagacgta ttacctaaga gttccatgac tttagtcttg
    12961 cggcgtgtca gctaaccaaa aaagagaaaa agaaaagtaa aaaattcgac tttctttttt
    13021 ctagtagtat ataaattttt gcatatagat aaagatcaag ataccgataa cggaagctag
    13081 attgtaatct attttagtga cagtcaggta tagccaagta actagaagat cagtggaacc
    13141 tgcaccataa aagtttgagt ttgtcggtcc gttgacagtt aaaagaaaaa tcacgttgaa
    13201 gttattgata tttccgatta cgccgcttcg atttggtcac gatccaaatt ttggataatc
    13261 cctgtagaaa ttagcatagt agctgggata ccaattcaca tattgacaaa gataacagag
    13321 acttttacat aaaacggatc aatcaggaaa ggaattgctc gatccagccc cagttttcca
    13381 gcaatgtgtt aactggccca gcgtcattca gcaaattatg catgatgagg agcgaaatga
    13441 actgcggaac agccattgtg ataacgacaa gttttttgga tcaatgtaaa aaacgtcagc
    13501 gaaaggaaaa atttcgtggt tttttatacc tgttcatttg gctagatata aaatccaaac
    13561 aaatgaacag aagtgcttat taggtttcaa ttgggagtat ctgcgctaga ataagagtaa
    13621 taatgaaaag gagtgttatg tatggattat tctagtaaag aatactttga taaaatgacg
    13681 gcttggtggc gtgctgctaa ttatctttct gttgggcaac tttatcttaa agataacccc
    13741 ttactgagac gtacactgaa acccgaagat gtcaaaaaac atccaatcgg tcattgggga
    13801 acgattcctg gccaaaattt tatttatgtc catttgaacc gagtcatcaa taaatatgat
    13861 ttgaacatgt tttatattga agggccagga cacggcggac aagtcatggt atcgaatgct
    13921 tatttagatg gaagttacac agagatctat ccagaagtaa cggaagatga gacaggaatg
    13981 caaaaattat tcaaacgatt ttcatttcct ggaggcattg cttctcatgc agcaccagaa
    14041 acaccaggtt ctatccatga aggcggagag cttggatact ctctttcaca tgcagtcgga
    14101 gctgtattag acaatcctga agtaatctct gctgtcgtga ttggtgatgg tgaagcagaa
    14161 accggtcctt tagctggaag ctggttctca aatgtcttta tcaatccagt gatagatgga
    14221 gctgttttac cgatccttca tttaaatggt gcaaaaatag ccaacccaac gatcttggca
    14281 cgtaaatcag atggagaatt agcaaattac tttaacggac ttggctggga acctttcttt
    14341 attgaaggaa atgattcaga aaaattaaat cctgttatgg ctgaaaaaat ggatcaagca
    14401 atcgagaaaa ttaaatccat ccaaaaagaa gctcgcctga agacagcaac agatgtcgtg
    14461 atgccaaaat ggccagtatt gatcgtacgc acaccaaaag gatggaccgg tccaaaagaa
    14521 tgggacgggg aaccaattga aggaaccttc cgagcacacc aagtaccgat tcctgtagat
    14581 caagaacata tggatcatgc agatgcttta ctccgttggc tgaaatccta tgaaccagaa
    14641 aaattatttg atgcacaagg acgtatttta gaagaaatca gagaaatcgc gcctactggt
    14701 gaccaaagaa tggcaaaaaa tcctatcacc aatggtggaa tcgatccaag accgcttatt
    14761 atgccagatt ggaaaaaata tactttgcaa tttgaaaaac caggctccgt taaagcagaa
    14821 gatatgaccg aacttggtaa attcgttcgc gagatcattg agaaaaatcc agaaaatttc
    14881 cgtatatttg gaccagatga aacaaaatca aaccgattga atcaagtttt caaaacgaca
    14941 aatcgccaat ggatggaaaa aatcgaacca gaaaatgatg aatggctttc gccatctggt
    15001 cgcgtcatcg acagtcaatt atcggaacac caagatgaag ggttcttaga agggtacgtt
    15061 ctgactggac ggcatggttt tttgcaagtt acgaatcatt cttgcgagtg gttgactcta
    15121 tgttgactca gcacttcaaa tggatgcgga aatcacatga cctttcttgg cgaaatgatt
    15181 atccttcgct aaatctcatt gcaagctcaa cagtcttcca gcaagatcac aatggatata
    15241 gccaccaaga tccaggaata ttgacacatt tggcagaaaa gaaggcggag ttcatccgtg
    15301 aatatctgcc tgcggatgca aatacattgc tcgcagtcat ggataaagca ttccgctcgt
    15361 cagaaaaaat caatttgatc atctcttcca aacatccgcg tgctcaattt tactcagcag
    15421 aagaagcagc cgtcttagtg aatgaaggct tgaaaatcat tgattgggct tcgacagcaa
    15481 aagaggagga acctgaattg gtcattgctg ctgcaggaac ggaatctaac ttagaagcat
    15541 tagcagctgt aactttgctg ctggaagaat tcccaaaact aaaaattcgg ttcatcaatg
    15601 tcgtagattt attgaaatta cgtcatccaa gtcaagatcc gcgtgggttg agcgatgaag
    15661 aatttgatca atattttaca aaagacaaac cgatcttatt tgctttccac ggttatgaaa
    15721 cattggtccg cacgattttc tttgatcgtc ataaccatca tttaatgatt catggctaca
    15781 aagaaaatgg agatatcacg acaccatttg acatgcgtgt agtaaatgaa cttgatcgtt
    15841 atcatttggc aaaagacgca gctttaaaaa tcaaaggaag tcaagcagaa gattttgcga
    15901 aaaaaatgga tcagaaatta caagaacatc aaaactatat tcgtgaaaat ggtattgatc
    15961 tgccagaagt attggattgg aaatggaaaa atcttgacca ataaaatgat aaaatcgatc
    16021 ccgcaaaata gaatttttta cattgattaa gaaactgaaa aaataaataa aaaaaacgga
    16081 aaaaagactg gaagaatggc cagtcttttt tttcgttttt gggaagtgaa aaccaaacta
    16141 tttacctgta agccacccat aaatccaatc aaatggtgca aagaaaaaat cttccattct
    16201 attccctcct ttaaaaaagc ataacatgat ttctatttac gtgagcatga attttgttac
    16261 gtttttattg ccgaaaaata aaaaccgttc attatgatgt caaattcgtc agtaattgat
    16321 gcaatgtgtt ataataattc agttaaatca aaaatgaaat ggagaaaaga catggacggg
    16381 ttattacctt tatggaaaga acgcggaatg acaagtcatg attgcgtatt caaattaaga
    16441 aaaatcttac atacgaaaaa aatcggacat gggggtacgc ttgacccaga cgtagacggt
    16501 gtgttgccca tttgtattgg aaaagccaca aaagttatcg aatatttgac agattctggg
    16561 aaaatctatg taggcgagat aactctaggt ttcagtacaa cgacggaaga tcgatccgga
    16621 gaacgggtag aaacaaaacc agtagatatg tctttatctg aaaaaaaaat agatgaagtg
    16681 atggcttcat ttattggtga gatcacccag ataccaccaa tgtactcagc ggtaaaagtc
    16741 aacggtaagc ggttatatga atatgcccgc aataatgaaa cagtggagcg tcctgttaga
    16801 aaggctcaga tttaccgttt tatccggaca agtgaattga caagagacga agtgacagga
    16861 accatttctt ggcgcttcga agtagaatgc gggaaaggaa cctatgtccg cacattagct
    16921 gtagatacag gcaagaaact tggttaccct gcgcacatgt ctgatctgac ccgaacagca
    16981 agtgccggta tgtcccagaa ggatgctctg acattagaag aagtagctca agctgtgaaa
    17041 aatgggacaa tcgatgattg tttgctgcca atagaaaaag gagtggaacg gttcaaacga
    17101 gtagatatta ctgatgaagt atggcagaaa gtaaaaaacg gtatgagact tgattatact
    17161 gtatttgcgc tcccagaaat gccgtcagaa gaaattgctt tgttttataa aggaaaagtt
    17221 gtcagtattt atcagccaaa cccgaaagaa aaaaacaaat taaaacctag taaagtattg
    17281 agaaatgagg aataacgaat gaatatcatc aagatcagac atccttacca atcagaacaa
    17341 attccagaag acgatgtcgt tttagtgctg ggatttttcg acggcgtgca tatcggacac
    17401 cagaaagtca taaagacagg tatggaaatt gcacaaaaag aaggattgaa attagcattg
    17461 atgactttca atcagcatcc atctattgtc tttaaaaaga tcgatcctag cagcgtgaaa
    17521 tatttgacta gccttaagca aaaagaagaa aaaatggccg ctttgggtat agattattta
    17581 tatgaagttg attttacttc ttcttttgct catttagcac cgcaggaatt tgttgatcaa
    17641 tacatcgtag ggttacatgc aaaatatgct gtttccggtt ttgattatac gtatggacca
    17701 aaagacattg cagatgtcgc tcattttcct gaatatgcca aaggaagatt caaaatcgtc
    17761 acagtttcta aagaagaaga acaaggtgaa aaaatcagct ctacccgtat tcgtggatta
    17821 ctagacagcg gacaagtaaa agaagcagca aaactattag gttgcacata tgaggtagaa
    17881 ggggtagttg tccatggcga tgcacgaggc agactactag gattccctac agcaaatgtc
    17941 aaagtaaaga gcacggttca tttgcctaaa gaaggtgttt atgtgtcgga aataaaaatt
    18001 ggggaaacat ggtatccagc catgggttca attggacata atgatacatt cggtgaagga
    18061 cgggagctga ctgtagagtt gtatattttg gactttaatc aagatatcta tggtgaacat
    18121 gtcgcggtac gctggaatca atttttaaga gatcaagtga aattttctag tgtagaggaa
    18181 ttgattgaac aactcaagga ggatgaacaa gcaacagctg aatatttttc tcagcaataa
    18241 aacattatgc ataaaatctc ttgtttatgt ataactagca attacaataa aatgggtgat
    18301 aaataagccc aggaaagggg ctgtgacaaa agtcttgtca cagccttttt ttccaaataa
    18361 atggtttttc agaagtagct tcttcggaaa taagccgaaa tttcttgaag ctgaccactt
    18421 ctgtcacaac ctctttcttt atagaaataa aaggacacta gcaattcatg aaaacgattg
    18481 ctagagcgta tcatttgctt tagactatat atcttggtgt acactcactt taaggcggaa
    18541 ataaaagtta tccgtaataa caggtttagg tatgaaaaag gagtgggtac tatgtacaag
    18601 gaattagaaa acaaagtcgc agtagtaact ggtggctcaa aggggattgg aacagcgatt
    18661 gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca actataactc ggataaagaa
    18721 ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg gaaaagcagt ctctgtacaa
    18781 gcagatgtca gttctgaaga aggaatacag cgtttacttg atacggcttt agaaaatttt
    18841 gggacactgg acgtttgggt taataatgct gggatggaaa atcagcatcc aacccatgaa
    18901 cttagccttg aagattggga aagagtgtta aaagtcaatt tgactggcgt ttttatcgga
    18961 acgaaagcgg ctttaaatta ctttacagag aataataaaa aaggaaatat catcaatatg
    19021 tcttctgttc atgaacagat cccatggcca acgtttgctc attacgctgc ttcaaaaggc
    19081 ggggtaaaat tatttaccga aacggtagca atggagtatg caccgaaggg aatcagaatc
    19141 aatagtatag gccctggtgc tatccgtaca cctattaatg cggcaaaatt tgatgatcct
    19201 gaacaaaaag aactcttgga aagtatgatc ccactaggac gcgtaggtga accaaaagaa
    19261 atagcggcga cagctgcatg gttggcttca gacgaatcca gttatgttac aggtattaca
    19321 ctatttgcag atggtgggat gactttatat ccttctttcc aaggtggaaa aggataaggg
    19381 tgaataaaaa tgggtttatt gattgcattg atcccggcag ttggctgggg gatccagcca
    19441 ctgattttga aaaagattgg tggcagacca acaaatgaga tattaggaac tggtattgga
    19501 gcggtgcttg tcggcttact cgttcaatta ttcgcttctc cagctggtat ttcagtgact
    19561 acatttttga ttagtttact ttctggtgca ttttgggtta ttggacaaac aggacagtat
    19621 acatctttca acttgattgg tgtttctaaa acgatgccaa tttcgactgc attacagtta
    19681 gtcggaacct cgttgattgg tgtcttcgct tttggagaat ggtctggaag tatggggaaa
    19741 atcattggag cagcagcaat cattcttctt gtatttggtt ctgctttaac ggcaatcagt
    19801 gatgatcctg gaaaaaaagg cggtatcaca aaaggaatta gtctattagc atcaacaagt
    19861 attggttatt gggtatacag tgcattgcct aaactagttg atgcaagcgg actttccatt
    19921 ttctttccgc aaatgatcgg tgtcttttta ggggcagtga tttatgtgat tttccgtaaa
    19981 ccaaccgcat tttctgaaga caagagttgg aaagcaacga tcgttggagc catctttagt
    20041 gtgtcagcat tggcttatat tttttcagca aatgaaaatg gggttgctac tgcatatatc
    20101 attacacagc taaacgtagt tatctctaca ttaggcggta tgtacatcct gcatgaaaat
    20161 aagacaccaa aagaaaaaaa attaacgatt atcggattat tgctgatcgt agtaggaagc
    20221 atggtcacgg tctttatttg aggataaaga tggtaaaatt tcatttatta aaaaaatatt
    20281 tgtttgaacc aaacagtaag tacttataaa aaaatagcgc tagaatttcc aacatctctg
    20341 gaatttctag cgctttttaa ttgtttaaga tacaagggaa atatctgtct ggatcaataa
    20401 tttttcattg atccattgac taaagtttac gacataattg aagttaccac cgattagtat
    20461 ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga cggtcttttt tatttgcgta
    20521 aacatcgtta tggtactgga ctaattgttc tcggaattga ttgattgctg taaaatcacg
    20581 atattgaagg ttgaaacgca tcgtgattgg tgcatttagc atgaatcgct gcatttcttt
    20641 tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa agattacgtt cagaaaattt
    20701 ccattgcccg ccaacctttg ttccttttag ttttccttca tgaagataat ttctgataga
    20761 acgtgtagta acacctaatt gttcggctaa ttcatttact gagtaaaaat ctttcaatgt
    20821 tcaccctcct atgtgaatgg ttctactgac ttagttgata tttgcttaat cacgaaatgt
    20881 agaaagaacg attttaatta aggtcattgt aaaaa
//
LOCUS       DACDID010000037        20484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDID010000037 DACDID010000000
VERSION     DACDID010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.037"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IV236_001071"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IV236_001071"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8771281.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IV236_001072"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IV236_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8771282.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IV236_001073"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IV236_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8771283.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IV236_001074"
     CDS             2856..3731
                     /locus_tag="IV236_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8771284.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IV236_001075"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IV236_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8771285.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IV236_001076"
     CDS             4628..5062
                     /locus_tag="IV236_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8771286.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IV236_001077"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IV236_001077"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8771287.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IV236_001078"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IV236_001078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8771288.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IV236_001079"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IV236_001079"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8771289.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IV236_001080"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IV236_001080"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8771290.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IV236_001081"
     CDS             13333..14259
                     /locus_tag="IV236_001081"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8771291.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IV236_001082"
     CDS             14441..15334
                     /locus_tag="IV236_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8771292.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IV236_001083"
     CDS             15468..15593
                     /locus_tag="IV236_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771293.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="IV236_001084"
     CDS             complement(15727..16359)
                     /locus_tag="IV236_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771294.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="IV236_001085"
     CDS             complement(16507..17049)
                     /locus_tag="IV236_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8771295.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="IV236_001086"
     CDS             17184..17573
                     /locus_tag="IV236_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771296.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="IV236_001087"
     CDS             17577..18341
                     /locus_tag="IV236_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8771297.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="IV236_001088"
     CDS             18470..19417
                     /locus_tag="IV236_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8771298.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="IV236_001089"
     CDS             19547..20287
                     /locus_tag="IV236_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771299.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DACDID010000038        20179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDID010000038 DACDID010000000
VERSION     DACDID010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.038"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..538
                     /gene="mraZ"
                     /locus_tag="IV236_001090"
     CDS             107..538
                     /gene="mraZ"
                     /locus_tag="IV236_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8771300.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            559..1518
                     /gene="rsmH"
                     /locus_tag="IV236_001091"
     CDS             559..1518
                     /gene="rsmH"
                     /locus_tag="IV236_001091"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8771301.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1523..1924
                     /gene="ftsL"
                     /locus_tag="IV236_001092"
     CDS             1523..1924
                     /gene="ftsL"
                     /locus_tag="IV236_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8771302.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1925..4117
                     /locus_tag="IV236_001093"
     CDS             1925..4117
                     /locus_tag="IV236_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8771303.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4143..5105
                     /locus_tag="IV236_001094"
     CDS             4143..5105
                     /locus_tag="IV236_001094"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8771304.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5119..6480
                     /locus_tag="IV236_001095"
     CDS             5119..6480
                     /locus_tag="IV236_001095"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8771305.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6506..7594
                     /gene="murG"
                     /locus_tag="IV236_001096"
     CDS             6506..7594
                     /gene="murG"
                     /locus_tag="IV236_001096"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8771306.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7609..8829
                     /locus_tag="IV236_001097"
     CDS             7609..8829
                     /locus_tag="IV236_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8771307.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8985..10310
                     /gene="ftsA"
                     /locus_tag="IV236_001098"
     CDS             8985..10310
                     /gene="ftsA"
                     /locus_tag="IV236_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8771308.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10333..11574
                     /gene="ftsZ"
                     /locus_tag="IV236_001099"
     CDS             10333..11574
                     /gene="ftsZ"
                     /locus_tag="IV236_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8771309.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11591..12268
                     /locus_tag="IV236_001100"
     CDS             11591..12268
                     /locus_tag="IV236_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8771310.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12282..12881
                     /locus_tag="IV236_001101"
     CDS             12282..12881
                     /locus_tag="IV236_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8771311.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12896..13183
                     /locus_tag="IV236_001102"
     CDS             12896..13183
                     /locus_tag="IV236_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8771312.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13232..14014
                     /locus_tag="IV236_001103"
     CDS             13232..14014
                     /locus_tag="IV236_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8771313.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14035..14742
                     /locus_tag="IV236_001104"
     CDS             14035..14742
                     /locus_tag="IV236_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8771314.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15004..17802
                     /gene="ileS"
                     /locus_tag="IV236_001105"
     CDS             15004..17802
                     /gene="ileS"
                     /locus_tag="IV236_001105"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8771315.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17851..19377)
                     /locus_tag="IV236_001106"
     CDS             complement(17851..19377)
                     /locus_tag="IV236_001106"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8771316.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19392..20039)
                     /locus_tag="IV236_001107"
     CDS             complement(19392..20039)
                     /locus_tag="IV236_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8771317.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6855 a         3502 c         4333 g         5489 t
ORIGIN      
        1 tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg
       61 attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga
      121 attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga
      181 acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat
      241 gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc
      301 acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg
      361 acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt
      421 catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc
      481 agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga
      541 ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa
      601 acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt
      661 gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt
      721 gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa
      781 ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat
      841 gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa
      901 gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca
      961 gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt
     1021 ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta
     1081 cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc
     1141 ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg
     1201 attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta
     1261 ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc
     1321 aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata
     1381 cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag
     1441 gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata
     1501 agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga
     1561 tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga
     1621 aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat
     1681 tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg
     1741 cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa
     1801 aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat
     1861 taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa
     1921 gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat
     1981 gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt
     2041 atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc
     2101 tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat
     2161 ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat
     2221 actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga
     2281 taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat
     2341 tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa
     2401 tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg
     2461 tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt
     2521 tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga
     2581 gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa
     2641 ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga
     2701 tatctacacg actttagaca gcagactaca aagttattta gaaacactga tggatcaagt
     2761 aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga
     2821 aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg
     2881 aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat
     2941 gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt
     3001 ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa
     3061 aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact
     3121 ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag
     3181 tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga
     3241 ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc
     3301 tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt
     3361 ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc
     3421 aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta
     3481 cggtagtgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac
     3541 agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc
     3601 gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca
     3661 ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc
     3721 aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc
     3781 cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg
     3841 actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg
     3901 tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc
     3961 tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga
     4021 agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat
     4081 cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga
     4141 gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg
     4201 caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg
     4261 agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc
     4321 tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta
     4381 ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt
     4441 ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac
     4501 agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat
     4561 tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc gctctcttct
     4621 ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg
     4681 gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg
     4741 atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca
     4801 gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag
     4861 cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg
     4921 ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa
     4981 tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga
     5041 ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt
     5101 ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc
     5161 ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt
     5221 gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct
     5281 ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca
     5341 ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa
     5401 atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt
     5461 attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg
     5521 aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt
     5581 ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa
     5641 ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca
     5701 catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa
     5761 aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta
     5821 gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt
     5881 gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa
     5941 cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa
     6001 ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca
     6061 catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca
     6121 accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta
     6181 ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc attaaaagga
     6241 atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa
     6301 gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta
     6361 gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa
     6421 tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag
     6481 aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg
     6541 acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga
     6601 attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat
     6661 gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa
     6721 gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca
     6781 accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc
     6841 caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa
     6901 atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt
     6961 tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa
     7021 aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt
     7081 tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata
     7141 tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct
     7201 aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc
     7261 agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga
     7321 attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca
     7381 tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa
     7441 tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt
     7501 gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct
     7561 ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa
     7621 aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa
     7681 aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa
     7741 gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa
     7801 cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag
     7861 aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa
     7921 cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg
     7981 atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc
     8041 aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat
     8101 gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca
     8161 agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca
     8221 gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat
     8281 ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag
     8341 ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa
     8401 ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc
     8461 caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa
     8521 atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa
     8581 gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg
     8641 gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca
     8701 gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat
     8761 gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat
     8821 gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat
     8881 ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt
     8941 tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa
     9001 tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg
     9061 acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca
     9121 ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag
     9181 aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag
     9241 tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag
     9301 atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg
     9361 tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg
     9421 gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca
     9481 tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta
     9541 ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg
     9601 tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt
     9661 ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa
     9721 atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac
     9781 ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag
     9841 tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag
     9901 caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag
     9961 gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg
    10021 taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa
    10081 gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta
    10141 cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg
    10201 agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag
    10261 aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg
    10321 gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa
    10381 gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt
    10441 aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct
    10501 gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca
    10561 gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt
    10621 gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca
    10681 atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca
    10741 ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa
    10801 gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa
    10861 aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa
    10921 ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa
    10981 acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat
    11041 cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat
    11101 ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca
    11161 caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt
    11221 acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc
    11281 gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca
    11341 cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa
    11401 aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat
    11461 tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa
    11521 tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga
    11581 ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg
    11641 tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga
    11701 atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt
    11761 agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt
    11821 aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca
    11881 tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc
    11941 ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga
    12001 gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat
    12061 gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa
    12121 agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag
    12181 tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt
    12241 aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag
    12301 gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc
    12361 ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa
    12421 ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg
    12481 taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac
    12541 ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa
    12601 atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg
    12661 ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac
    12721 aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca
    12781 aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa
    12841 ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta
    12901 tactattgca ctattacttc atttacttca acgggcagcc tatttctata gcattatctt
    12961 gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt
    13021 attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg
    13081 acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt
    13141 agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct
    13201 ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg
    13261 ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca
    13321 atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact
    13381 gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag
    13441 aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc
    13501 attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg
    13561 aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga
    13621 tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa
    13681 gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa
    13741 agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc
    13801 tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa
    13861 agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag atattatttc
    13921 agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa
    13981 gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct
    14041 ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa
    14101 gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga
    14161 aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa
    14221 ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa
    14281 acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag
    14341 ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca
    14401 agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat
    14461 cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta
    14521 gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact
    14581 gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact
    14641 gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct
    14701 attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt
    14761 tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc
    14821 ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt
    14881 ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt
    14941 cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt
    15001 tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat
    15061 ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa
    15121 agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc
    15181 aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt
    15241 tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg
    15301 ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa
    15361 tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc
    15421 aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat
    15481 gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga
    15541 ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa
    15601 tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat
    15661 attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca
    15721 aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt
    15781 aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat
    15841 gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca
    15901 ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact
    15961 ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat
    16021 aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga
    16081 tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa
    16141 ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa
    16201 aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag
    16261 aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg
    16321 tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta
    16381 ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa
    16441 catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa
    16501 gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa
    16561 aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag
    16621 cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc
    16681 tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca
    16741 gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga
    16801 aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg
    16861 gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg
    16921 gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat
    16981 ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg
    17041 accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac
    17101 ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac
    17161 ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc
    17221 atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt
    17281 ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta
    17341 agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact
    17401 gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa
    17461 ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa
    17521 atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc agactacttt
    17581 gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct
    17641 attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat
    17701 gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac
    17761 ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg
    17821 acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata
    17881 ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc
    17941 tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc
    18001 ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt
    18061 cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc
    18121 aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc
    18181 tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag
    18241 aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt
    18301 aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc
    18361 tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc
    18421 gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa
    18481 ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc
    18541 ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt
    18601 ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt
    18661 ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc
    18721 gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc
    18781 aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata
    18841 atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc
    18901 aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt
    18961 ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc
    19021 catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga
    19081 caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa
    19141 atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt
    19201 gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa
    19261 atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg
    19321 ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg
    19381 gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg
    19441 attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc
    19501 tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca
    19561 tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg
    19621 cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca
    19681 ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat
    19741 acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact
    19801 tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat
    19861 tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt
    19921 tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc
    19981 gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata
    20041 ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa
    20101 ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga
    20161 aagatgatag agaagactc
//
LOCUS       DACDID010000039        19495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDID010000039 DACDID010000000
VERSION     DACDID010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.039"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IV236_001108"
     CDS             68..751
                     /locus_tag="IV236_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8771318.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IV236_001109"
     CDS             753..1589
                     /locus_tag="IV236_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8771319.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IV236_001110"
     CDS             1600..3354
                     /locus_tag="IV236_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771320.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IV236_001111"
     CDS             complement(3630..3971)
                     /locus_tag="IV236_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8771321.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IV236_001112"
     CDS             complement(4039..6210)
                     /locus_tag="IV236_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8771322.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IV236_001113"
     CDS             6421..7278
                     /locus_tag="IV236_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8771323.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IV236_001114"
     CDS             complement(7349..8206)
                     /locus_tag="IV236_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8771324.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IV236_001115"
     CDS             complement(8191..10893)
                     /locus_tag="IV236_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8771325.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IV236_001116"
     CDS             complement(10906..12195)
                     /locus_tag="IV236_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8771326.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IV236_001117"
     CDS             12345..13391
                     /locus_tag="IV236_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8771327.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IV236_001118"
     CDS             13393..15546
                     /locus_tag="IV236_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8771328.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="IV236_001119"
     CDS             complement(16019..17470)
                     /locus_tag="IV236_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771329.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="IV236_001120"
     CDS             complement(17467..19191)
                     /locus_tag="IV236_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8771330.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..>19495)
                     /locus_tag="IV236_001121"
     CDS             complement(19184..>19495)
                     /locus_tag="IV236_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8771331.1"
                     /translation="FLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISV
                     FSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESY
                     E"
BASE COUNT         5517 a         4025 c         3515 g         6438 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccctca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaa
//
LOCUS       DACDID010000040        19120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDID010000040 DACDID010000000
VERSION     DACDID010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.040"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IV236_001122"
     CDS             complement(<1..703)
                     /locus_tag="IV236_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771332.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IV236_001123"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IV236_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IV236_001124"
     CDS             1758..2837
                     /locus_tag="IV236_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8771333.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IV236_001125"
     CDS             2869..3462
                     /locus_tag="IV236_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771334.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IV236_001126"
     CDS             complement(3572..3799)
                     /locus_tag="IV236_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8771335.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IV236_001127"
     CDS             complement(3902..6490)
                     /locus_tag="IV236_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8771336.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IV236_001128"
     CDS             complement(6510..7169)
                     /locus_tag="IV236_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8771337.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV236_001129"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV236_001129"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8771338.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IV236_001130"
     CDS             complement(7942..8808)
                     /locus_tag="IV236_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8771339.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IV236_001131"
     CDS             8917..9558
                     /locus_tag="IV236_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8771340.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV236_001132"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV236_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8771341.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IV236_001133"
     CDS             complement(10999..11898)
                     /locus_tag="IV236_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8771342.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IV236_001134"
     CDS             complement(11900..12697)
                     /locus_tag="IV236_001134"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8771343.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IV236_001135"
     CDS             complement(12675..13379)
                     /locus_tag="IV236_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8771344.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IV236_001136"
     CDS             13510..14085
                     /locus_tag="IV236_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8771345.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IV236_001137"
     CDS             14203..14880
                     /locus_tag="IV236_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8771346.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV236_001138"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV236_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8771347.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IV236_001139"
     CDS             complement(16943..17941)
                     /locus_tag="IV236_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8771348.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IV236_001140"
     CDS             complement(18167..18820)
                     /locus_tag="IV236_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8771349.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5522 a         4136 c         3323 g         6139 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
//
LOCUS       DACDID010000041        18976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDID010000041 DACDID010000000
VERSION     DACDID010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.041"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            26..682
                     /locus_tag="IV236_001141"
     CDS             26..682
                     /locus_tag="IV236_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8771350.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1124..1921)
                     /locus_tag="IV236_001142"
     CDS             complement(1124..1921)
                     /locus_tag="IV236_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8771351.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1918..3102)
                     /locus_tag="IV236_001143"
     CDS             complement(1918..3102)
                     /locus_tag="IV236_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8771352.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3127..4374)
                     /locus_tag="IV236_001144"
     CDS             complement(3127..4374)
                     /locus_tag="IV236_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8771353.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4589..6334
                     /gene="dnaX"
                     /locus_tag="IV236_001145"
     CDS             4589..6334
                     /gene="dnaX"
                     /locus_tag="IV236_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8771354.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6354..6668
                     /locus_tag="IV236_001146"
     CDS             6354..6668
                     /locus_tag="IV236_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8771355.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6693..7289
                     /gene="recR"
                     /locus_tag="IV236_001147"
     CDS             6693..7289
                     /gene="recR"
                     /locus_tag="IV236_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8771356.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7353..7997
                     /locus_tag="IV236_001148"
     CDS             7353..7997
                     /locus_tag="IV236_001148"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8771357.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8012..8341
                     /locus_tag="IV236_001149"
     CDS             8012..8341
                     /locus_tag="IV236_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771358.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8355..9293
                     /gene="holB"
                     /locus_tag="IV236_001150"
     CDS             8355..9293
                     /gene="holB"
                     /locus_tag="IV236_001150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8771359.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9329..10153
                     /locus_tag="IV236_001151"
     CDS             9329..10153
                     /locus_tag="IV236_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8771360.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10146..10493
                     /locus_tag="IV236_001152"
     CDS             10146..10493
                     /locus_tag="IV236_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8771361.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10562..11434
                     /gene="rsmI"
                     /locus_tag="IV236_001153"
     CDS             10562..11434
                     /gene="rsmI"
                     /locus_tag="IV236_001153"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8771362.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11543..12664
                     /gene="dinB"
                     /locus_tag="IV236_001154"
     CDS             11543..12664
                     /gene="dinB"
                     /locus_tag="IV236_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8771363.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12718..13320)
                     /gene="nrdG"
                     /locus_tag="IV236_001155"
     CDS             complement(12718..13320)
                     /gene="nrdG"
                     /locus_tag="IV236_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8771364.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13635..15824)
                     /gene="nrdD"
                     /locus_tag="IV236_001156"
     CDS             complement(13635..15824)
                     /gene="nrdD"
                     /locus_tag="IV236_001156"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8771365.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16150..16917
                     /locus_tag="IV236_001157"
     CDS             16150..16917
                     /locus_tag="IV236_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771366.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16907..18949
                     /locus_tag="IV236_001158"
     CDS             16907..18949
                     /locus_tag="IV236_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771367.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5937 a         3463 c         3951 g         5625 t
ORIGIN      
        1 tgaaagtttg gatgggggag catcattgga tagtagagtc ttaaaggaat atttggataa
       61 aaatgatttt ccgattatac gcaaaggata tcataaatat ttgacattcg aagggatgga
      121 agattcccat acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga
      181 tgggagagaa tttaaccttc gctatatcaa agatcttgaa atcgtctctc ttttaaaaga
      241 cgaatattca caattcatcg attcgccgta taatattcga gttgaatcag aatatgcaga
      301 gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc caaagctaca
      361 acactatgta aaagagtatt accgctatcg tctgctttat tcgatgaaaa aaatgcagca
      421 aatgctatta aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat tatatgattt
      481 attcggtgtg cggacagaag aagggatgct gatcgatttt tcgatcacga atgaagaaat
      541 cgccaaattt tgtggcatct caactgctag cagtgtaaac agaattttaa aacaactgaa
      601 aaaagatggt attatcaccg ttaaagacca tcatatcgtt attttgaata tggacttgtt
      661 agaagacaat attatctttt gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag
      721 tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
      781 gagggtcagc tgattgaaca ccgtttacca agaggttgtg agcctgccgt ttaactctga
      841 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
      901 ccgaggagtt ggtaggagaa caccgtttac caagaggttg tgaaaaagct gttctgcatc
      961 aagtaataag aagagccaag taaaaacagt ctcttatgtt tttaacttgg ctggacttat
     1021 caccgtagat gtaagctttt gaacgccgtt tatttgcaac aaaaggggct gtgacacgat
     1081 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
     1141 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
     1201 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
     1261 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
     1321 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
     1381 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
     1441 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
     1501 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
     1561 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
     1621 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
     1681 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
     1741 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
     1801 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
     1861 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaac tttttcatca
     1921 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
     1981 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
     2041 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     2101 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     2161 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     2221 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     2281 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     2341 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     2401 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     2461 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     2521 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     2581 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     2641 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     2701 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     2761 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     2821 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     2881 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     2941 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     3001 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     3061 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     3121 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     3181 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     3241 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     3301 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     3361 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     3421 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     3481 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     3541 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     3601 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     3661 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     3721 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     3781 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     3841 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     3901 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     3961 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     4021 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     4081 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     4141 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     4201 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     4261 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     4321 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     4381 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     4441 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     4501 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     4561 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     4621 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     4681 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     4741 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     4801 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     4861 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     4921 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     4981 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     5041 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     5101 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     5161 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     5221 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     5281 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     5341 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     5401 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     5461 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     5521 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     5581 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     5641 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     5701 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     5761 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     5821 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     5881 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     5941 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     6001 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     6061 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     6121 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     6181 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     6241 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     6301 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     6361 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     6421 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     6481 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     6541 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     6601 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     6661 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     6721 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     6781 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     6841 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     6901 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     6961 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     7021 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     7081 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     7141 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     7201 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     7261 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     7321 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     7381 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     7441 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     7501 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     7561 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     7621 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     7681 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     7741 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     7801 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     7861 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     7921 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     7981 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     8041 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     8101 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     8161 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     8221 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     8281 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     8341 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     8401 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     8461 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     8521 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     8581 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     8641 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     8701 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     8761 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     8821 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     8881 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     8941 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     9001 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     9061 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     9121 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     9181 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     9241 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     9301 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     9361 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     9421 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     9481 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     9541 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     9601 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     9661 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     9721 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     9781 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     9841 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     9901 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     9961 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
    10021 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
    10081 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
    10141 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
    10201 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
    10261 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
    10321 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
    10381 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
    10441 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
    10501 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
    10561 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
    10621 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
    10681 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
    10741 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
    10801 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
    10861 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
    10921 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
    10981 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
    11041 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    11101 attgacgaat tttttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    11161 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    11221 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    11281 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    11341 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    11401 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    11461 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    11521 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    11581 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    11641 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    11701 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    11761 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    11821 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    11881 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    11941 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    12001 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    12061 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    12121 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    12181 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    12241 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    12301 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    12361 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    12421 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    12481 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    12541 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    12601 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    12661 ataaaaacag ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa gctgttttta
    12721 tatcaagtct tgctttttga tttgttcata ggtttctgtt gcatccgtac atttttccca
    12781 aatcacaact tgattggatt ctaaagattt ttggacgtca atgatccgct ggttgctact
    12841 tccgcgaaat tgtagtttta ggtcacgttt gcttaattca aagcgtccgt caacaaggat
    12901 atcgatttgc gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt catcaaaggc
    12961 atagcctgtc catgaccaga tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac
    13021 gacttgcaga caaacatctg tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg
    13081 cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg tgaatggttt
    13141 gccatatcgg aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc
    13201 actaacgtaa agactgttgc ggacaccttc accatcgaca aaattaaacg gtttgtagtc
    13261 agcaatatag ttttggctgt agtcttttgc taaccattct tttggttttg gatttctcat
    13321 aaaaaaactt ctttcattta aggttcaaag atatagctat tgaagataaa atctactcga
    13381 ccacataaat aagaacaaac tgacctaaat ccattgttca agaaagcttg ttcttatttt
    13441 gatagagggc gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa agacttgtca
    13501 tagcctctta ttctgaataa acggtgttca aaagcttgca tctgcagtaa taagcccaac
    13561 taaacgaaaa atatgaggaa ctatttttgt ttagctattc ttattacttg atgcaggaca
    13621 gctttttcac aacctcattt catgtgtttc acacgtgagg aaatttcttt atggcggcca
    13681 tgtaccatcg gacgagcttg cggatttcct aaatagccac aagtacgttt gactacatca
    13741 catgtttttg gatcatgatt tccacattct ggacattcga accctcgttc tgtcggtttg
    13801 aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt accaagatac
    13861 ccgattttgt catatgcaaa atcccataca gcttctaaag ctttcggatt ttgttggagc
    13921 atagggtatt cacagtagtg gatgaatcca cctgaacaat attttggata atccttctca
    13981 aattccaatt tttcaaatgg cgttgggttt ttacgcacat catagtggaa actgtttgta
    14041 taatattctt tatctgtgat attttcgatg atgccgaatt tttctgtatc caaacggcag
    14101 aaacggtcag ttagactttc acttggagtt gaatagacac tgaaatgata accatattca
    14161 tccccccatg catctgcatt agcttttaat tctttgacga tatcaagtgt gaaagctttt
    14221 gcttctgggt ttgtttccca ttcgccaccg aagaaagctg aagcaacttc atacaatcca
    14281 atgtagccta acgatacagt agctcgacgg tttttgaata attcattgac agaatcggta
    14341 cgtgaaagac gtttcccgaa agaaccatac atatataaaa ttggcgcatt tgctggaatg
    14401 gcttctttac aacgttctac acgatagaga agcgcatctt tcatgatttc taagcggtca
    14461 gacagtaact gccagaattt attctgatct ccttttgatt cgatcgcgat tcttggcaaa
    14521 ttcaatgtaa caacacctaa attcattcgt ccgacattta cttcttgacc attttcgtct
    14581 ttccaccctt gcaagaatga acgacagccc ataggtactt tgaaacttcc agttaattca
    14641 acgattttat cgtaattcag aatatcagga tacattcttt tcgttgcaca ttccaatgct
    14701 agttgtttga tgtcgtaatt aggatcttca gggtttaagt tcacaccttt tttcaaagtg
    14761 aagatcagtt tagggaagat agctgtccgc ttttcactcc ctaatccatt gattcgaatc
    14821 tgtaaaattg ctttttgaat ttctctttca aaccagtttt ctcctagtcc aaatcctaat
    14881 gaagtgaaag gagtttgtcc atttgaagta aataaggtat tgatttcata ttccaaactt
    14941 tgcatagcat cgaagatatc ttttttggtt tttgcttttg catattcttt ttggcgttct
    15001 ggcgaatcga tccattcttc cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa
    15061 ggagcaagca attcatctgt tcggtcggca gaacagccac catattgact tgacgctaca
    15121 ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga tagattttgg cgattcgact
    15181 tcggcattgc cgattttgaa cccattgttc agcatgcctt tgaaatcaat caagcagcaa
    15241 ttcgtcatcg gtgtataagg atgataatcc aaatcatggt aatggatatc gcctttttga
    15301 tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga ttgattttcc aacgatcccg
    15361 gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg
    15421 acagtttggt ccttgttgat tagcttttca atcgaaaagt tgatatctgt tgctttactg
    15481 cgagcaaaat ctcgtttggt acgataattg atgtactctt ctgctaattc atattcgttt
    15541 ttttctagta atatatgttc cacgatgttt tgaatctcat agattttgac attttcagca
    15601 aaccggcttt tgatttcttg gacaaccaag tctgcaatca tttgaatttt ttcataagta
    15661 agtggtgata atgagccgtg aattttcttt tctgctttga tcagtgcttc attgatcttt
    15721 tgttcatcga aatcaacatg gcggccatcg cgtttgatga ctttcatctg ggatgtctct
    15781 ggctgctgag taacgacagt tttttcttca taatttactt tcattatctt caactcctgc
    15841 taaattgttc ttgtctatca taaaacaaaa tcggggaagg agcaactaaa tatcggctat
    15901 atatagtatt caatgtgctg tataattgga tttctatact atatgtagtg tatattttat
    15961 tgtgaaaaaa ggtgaaaact tgtagctggt ggatttgttt tttgcatatt taattttgct
    16021 gaataaaata attgaataaa attttttatt cagtccaagt aaaagtgaca aaaacataag
    16081 agcaaattat tcgtagatat gctactatgc aaataaatag aaatgaaaaa tgaaagagga
    16141 ggagaagaat tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa
    16201 ctttctcccg tcaaagcctt agataatatc tcattcacag tagaagaagg cgagtttgtt
    16261 gggattatgg gcccaagtgg tgcaggaaag acaacgctga tgaatatctt gtccacgatc
    16321 aatttgccta cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac
    16381 agtgagctca gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg
    16441 atcgataccc taaatgcaaa agacaatatc ctattgcccc tagcagttga acggatgact
    16501 aaagaggaaa tggaaaagag agtaagacat gtagctcaat tgttgaatat agaagaactg
    16561 ctcaaacggt atcccgatga actttctgtc ggacagagac aacgaattgc agctgctaga
    16621 gcacttgttg ttcagccaca agtgattttt gcagatgaac caactggctc tttggactcg
    16681 aagtcggcta cagaattgtt gaactacttg aagaccatga atcaaaaaga aaaagcaacg
    16741 atactgttag tcacccatga tccatataca gcaagctact gcgatagaat tttatttatt
    16801 aaagatggtg tgattttttc tgaagttgtc agaagaggga caagaagaga gttctttgag
    16861 aaagtaatag acatgcaggc gacgattggc ggaggaggaa aaatgaatgc tgtataaact
    16921 agccgttaaa agtttttttc ggcagtcacg aggctatctt gtttattttt tcagtttgac
    16981 tttatccgta atgatctatt attccttcag cgcaatgacc tacgatcaat ctttagtcag
    17041 aagagcaagt caagatactt cgatagatgg tggattgagt tttggtggat ttatcattac
    17101 tgttgttttg ctttttttcg tattcagtgc caaccgattt ttcttgaata gaaggcaaaa
    17161 ggaaataggc atctatcagc tttttggtat gaataaactg cagatatcag gtatatatgt
    17221 gttagaaatc atgataattg gattgttcgc ctgtatttct gggatattac tcggtattat
    17281 tttttccaaa ctcttttcaa tgattttggt tcgaatgatg gatatggatc ttactagtcc
    17341 attttttatc tccattccat cagtagtaga tactttattt gcttttttca tcatcctttt
    17401 agcagtatct gtatattcaa tatggaaaat atggcgctat ccaatgattc gaagttttgg
    17461 agaaaaagaa caagcagata gttcaacaat gcgcttcagg acaaggcacc gattactcgc
    17521 agtcatcggg ttactacttt taacgagcgg atattttgga gctagtcatt ttagggaaat
    17581 cacttcatgg atgatcagtt atcattatct tgatacatta gttttctttt ttcctttttt
    17641 aatttttttc gtttgtgtgg tcggtactta tttgtttttc tgttatacat ttcggttatt
    17701 tttgatcata ttgagtaaat ggaagattta ttactatggt atcaattttt tgataatggg
    17761 taacacgcaa gttcatttgc tcaaaagttg gcgtacaaat tctctgatta cagtcattct
    17821 cggaatttct ttaatgacaa ttggcggcac ggctagtatc tctactattt tatcctacca
    17881 ggagaaagtt tcctccccga tggactatca gtttgatgtt gagacagaga aaaaaatcaa
    17941 gccgattcta gcagaggaag ggcagaagat aactaaagaa atcaccttgc attataaagt
    18001 cattgggagt atctatagtc aaaatctcga agaacctatg tttcaaactg ctaatctgat
    18061 cacggaaaca caatatcaag ccttcagaaa agttaatcca gatttgccga acatttcttt
    18121 gaaatcacct ggtcatactg ttttgcttga tcaaatgcaa actctttttc ggaatatttc
    18181 attatatgga tctagtattg gactgccaga taatcaagaa ttgaagattc aacaaatgct
    18241 tccaagtgta ttaggtgacg aaacaatggt atatctaagt cctgtactga ttgtagatga
    18301 taccgttttt gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg
    18361 tggaaataat gaaaaaatag atgaacgact gagcaatgac ctgtcgataa agtggctcaa
    18421 tgctgtttat tattcttatg atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa
    18481 gaaacttccg gatgaccaaa tgaaggaagc acaatttagc caagaaagct ctcgtttgaa
    18541 tttctcttcc agctattcga aattgcgtgc agttaatcgg cgaatcggta tcaatatttt
    18601 tgtcacacta tttgtcggga tgatcgtgat tattgctact ggtagtattt tgacagtccg
    18661 gcaattagca gaagcagagg aagaaaaaga aaattatcac ctattgacta agctggggat
    18721 tccacaaaga aggatcagac ggatgatctt cgagcaaaac gcacttacct tctttccacc
    18781 catgatatta gggatcactc acgcagtatt tgcaatcaat gtcttcactc agtatataaa
    18841 aactgccgat tactggttgg cttatcttgt gagtggctta ttgatcctta tctatctttt
    18901 gctttatgtc attacgagtc agttatattg tcggattatc gaggaatgat atacggaata
    18961 agacaagata ataaat
//
LOCUS       DACDID010000042        18844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDID010000042 DACDID010000000
VERSION     DACDID010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.042"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..736)
                     /locus_tag="IV236_001159"
     CDS             complement(26..736)
                     /locus_tag="IV236_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771368.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(754..1251)
                     /locus_tag="IV236_001160"
     CDS             complement(754..1251)
                     /locus_tag="IV236_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771369.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(1288..1740)
                     /locus_tag="IV236_001161"
     CDS             complement(1288..1740)
                     /locus_tag="IV236_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8771370.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(1875..2480)
                     /locus_tag="IV236_001162"
     CDS             complement(1875..2480)
                     /locus_tag="IV236_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8771371.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2930..3550)
                     /locus_tag="IV236_001163"
     CDS             complement(2930..3550)
                     /locus_tag="IV236_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8771372.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3552..4421)
                     /locus_tag="IV236_001164"
     CDS             complement(3552..4421)
                     /locus_tag="IV236_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8771373.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4444..4851)
                     /locus_tag="IV236_001165"
     CDS             complement(4444..4851)
                     /locus_tag="IV236_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8771374.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4870..6159)
                     /locus_tag="IV236_001166"
     CDS             complement(4870..6159)
                     /locus_tag="IV236_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8771375.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6186..6488)
                     /locus_tag="IV236_001167"
     CDS             complement(6186..6488)
                     /locus_tag="IV236_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8771376.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6513..8606)
                     /locus_tag="IV236_001168"
     CDS             complement(6513..8606)
                     /locus_tag="IV236_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8771377.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9095..9859)
                     /locus_tag="IV236_001169"
     CDS             complement(9095..9859)
                     /locus_tag="IV236_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8771378.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9970..10839)
                     /gene="mdcB"
                     /locus_tag="IV236_001170"
     CDS             complement(9970..10839)
                     /gene="mdcB"
                     /locus_tag="IV236_001170"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8771379.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(10864..11940)
                     /locus_tag="IV236_001171"
     CDS             complement(10864..11940)
                     /locus_tag="IV236_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8771380.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(11964..12557)
                     /locus_tag="IV236_001172"
     CDS             complement(11964..12557)
                     /locus_tag="IV236_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8771381.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(12561..14237)
                     /gene="mdcD"
                     /locus_tag="IV236_001173"
     CDS             complement(12561..14237)
                     /gene="mdcD"
                     /locus_tag="IV236_001173"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8771382.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14227..14526)
                     /locus_tag="IV236_001174"
     CDS             complement(14227..14526)
                     /locus_tag="IV236_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8771383.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(14628..16274)
                     /gene="mdcA"
                     /locus_tag="IV236_001175"
     CDS             complement(14628..16274)
                     /gene="mdcA"
                     /locus_tag="IV236_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8771384.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(16281..17192)
                     /locus_tag="IV236_001176"
     CDS             complement(16281..17192)
                     /locus_tag="IV236_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8771385.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            17312..18169
                     /locus_tag="IV236_001177"
     CDS             17312..18169
                     /locus_tag="IV236_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8771386.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(18356..18718)
                     /locus_tag="IV236_001178"
     CDS             complement(18356..18718)
                     /locus_tag="IV236_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771387.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         5240 a         4003 c         3269 g         6332 t
ORIGIN      
        1 tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg
       61 tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg
      121 cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt
      181 gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag cgacagatcg
      241 ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac
      301 ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct
      361 tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat
      421 ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt
      481 caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag
      541 caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg
      601 cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac
      661 catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca
      721 ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata
      781 gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt
      841 tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt
      901 tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg
      961 atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc
     1021 ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact
     1081 cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata
     1141 agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt
     1201 aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat
     1261 ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa
     1321 attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc
     1381 ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat
     1441 gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc
     1501 tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt
     1561 cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc caggattttc
     1621 gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat
     1681 attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat
     1741 ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg caaatgcaac
     1801 tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc
     1861 ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt
     1921 ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg
     1981 ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa
     2041 acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact
     2101 gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca
     2161 ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc
     2221 cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact
     2281 tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct
     2341 tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta
     2401 tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat
     2461 tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat
     2521 caagtataga agactggttt aattacggca aataaatata atgaaatctg caataaataa
     2581 aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat
     2641 ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct
     2701 attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact
     2761 aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa
     2821 gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc
     2881 ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt
     2941 cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg
     3001 ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga
     3061 tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt
     3121 caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt
     3181 tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat
     3241 atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact
     3301 cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag
     3361 gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt
     3421 ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc
     3481 gtagtttatt cataagatat gtttttcctg taccaggact accagccaga agaatgaaat
     3541 actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca
     3601 atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga
     3661 gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa
     3721 agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct
     3781 tctttcacac ctgttcctcc atggataact agggggacat caactactgc atcgatttct
     3841 ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata
     3901 gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt
     3961 gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca
     4021 agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc
     4081 aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg
     4141 tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca
     4201 accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat
     4261 ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct
     4321 agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac
     4381 tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc
     4441 tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt
     4501 gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg
     4561 gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact
     4621 cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac
     4681 ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa
     4741 ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt
     4801 tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc
     4861 tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc
     4921 ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat taaagatcgt
     4981 tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc
     5041 tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt
     5101 ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat
     5161 aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa
     5221 gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg
     5281 aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc
     5341 cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa
     5401 ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac
     5461 gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc
     5521 ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa
     5581 taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg
     5641 aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc
     5701 gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt
     5761 tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc
     5821 caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc
     5881 caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg
     5941 aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc
     6001 ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc
     6061 tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg
     6121 agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt
     6181 tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt
     6241 tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg
     6301 aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa
     6361 gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca
     6421 gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat
     6481 ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt
     6541 tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg
     6601 atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa
     6661 caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc
     6721 gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc
     6781 atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg
     6841 acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat
     6901 tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt
     6961 aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct
     7021 aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt
     7081 tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt
     7141 cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact
     7201 aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat
     7261 ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac
     7321 ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg
     7381 actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg
     7441 atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta
     7501 ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc
     7561 aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta
     7621 tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct
     7681 acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag
     7741 atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg
     7801 acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct
     7861 tttatttctg tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg
     7921 atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac
     7981 aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga
     8041 aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc
     8101 agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa
     8161 accagtcctt gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt
     8221 aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg
     8281 tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg
     8341 cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt
     8401 tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc cggaagcttt
     8461 ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca
     8521 ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga
     8581 agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt
     8641 tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa
     8701 agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta
     8761 ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat
     8821 caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta
     8881 ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca
     8941 gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc
     9001 ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc tgcgacaagg
     9061 cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa
     9121 aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat
     9181 actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat
     9241 cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc
     9301 ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt
     9361 gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag
     9421 gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact
     9481 gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt
     9541 tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag
     9601 ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc
     9661 cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac
     9721 caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga
     9781 cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta
     9841 aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat
     9901 gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt
     9961 ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc
    10021 ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat
    10081 ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg
    10141 actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa
    10201 tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac
    10261 atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat gcggataccc
    10321 taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg
    10381 agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc
    10441 aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat
    10501 caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag
    10561 cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat
    10621 agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga
    10681 cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa
    10741 tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag
    10801 ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta
    10861 ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa
    10921 agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa
    10981 ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa
    11041 atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt
    11101 tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact
    11161 ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa
    11221 tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct
    11281 gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa
    11341 actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg
    11401 tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca
    11461 tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa
    11521 ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata
    11581 tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag
    11641 ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt
    11701 caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa
    11761 aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt
    11821 tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt
    11881 tacagctgat actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat
    11941 ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact
    12001 gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct
    12061 tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc
    12121 tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct
    12181 tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta
    12241 catcctgata atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt
    12301 ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg
    12361 gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc
    12421 ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt
    12481 agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata
    12541 tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta
    12601 tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc
    12661 gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg
    12721 acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct
    12781 agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg
    12841 atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg
    12901 gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat
    12961 gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt
    13021 tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta
    13081 tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg
    13141 tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa
    13201 ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc
    13261 acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa
    13321 gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct
    13381 ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt
    13441 gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat
    13501 aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg
    13561 acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct
    13621 acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct
    13681 cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt
    13741 gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca
    13801 cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg
    13861 atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct
    13921 agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt
    13981 gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc
    14041 atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca
    14101 tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga
    14161 tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct
    14221 acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac
    14281 tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa
    14341 aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt
    14401 tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac
    14461 cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg
    14521 ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt
    14581 cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt
    14641 ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt
    14701 tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat
    14761 ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga
    14821 ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg
    14881 tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc
    14941 actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca
    15001 aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct
    15061 ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga
    15121 ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt
    15181 acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg
    15241 gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg
    15301 acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa
    15361 ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc
    15421 aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt
    15481 aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt
    15541 tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt
    15601 tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg
    15661 tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc gttgacttga
    15721 acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta
    15781 ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca
    15841 tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct
    15901 aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa
    15961 gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag
    16021 atactagaca tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt
    16081 tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga
    16141 gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc
    16201 aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg
    16261 tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata
    16321 ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc
    16381 agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt
    16441 gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta
    16501 cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta
    16561 gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc
    16621 aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt
    16681 ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc
    16741 ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt
    16801 tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa
    16861 gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa
    16921 gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc
    16981 tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc
    17041 tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca
    17101 ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg
    17161 ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt
    17221 tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca
    17281 taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa
    17341 aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc
    17401 tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca
    17461 tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa
    17521 gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt
    17581 tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat
    17641 ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga
    17701 tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc
    17761 tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga
    17821 acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc
    17881 cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc
    17941 gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt
    18001 tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa
    18061 agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt
    18121 gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag
    18181 ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt
    18241 cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct
    18301 tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt
    18361 gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac
    18421 gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt
    18481 ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc
    18541 tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc
    18601 attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt
    18661 ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct
    18721 gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag aatagaaaaa
    18781 tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc aactcttaaa
    18841 tgtt
//
LOCUS       DACDID010000043        18729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDID010000043 DACDID010000000
VERSION     DACDID010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.043"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            246..521
                     /locus_tag="IV236_001179"
     CDS             246..521
                     /locus_tag="IV236_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771388.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            534..2606
                     /locus_tag="IV236_001180"
     CDS             534..2606
                     /locus_tag="IV236_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8771389.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2771..4657
                     /locus_tag="IV236_001181"
     CDS             2771..4657
                     /locus_tag="IV236_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8771390.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4669..5616
                     /locus_tag="IV236_001182"
     CDS             4669..5616
                     /locus_tag="IV236_001182"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8771391.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5636..6163
                     /locus_tag="IV236_001183"
     CDS             5636..6163
                     /locus_tag="IV236_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8771392.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6226..6879)
                     /locus_tag="IV236_001184"
     CDS             complement(6226..6879)
                     /locus_tag="IV236_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8771393.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7011..7853
                     /locus_tag="IV236_001185"
     CDS             7011..7853
                     /locus_tag="IV236_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8771394.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8011..8907
                     /locus_tag="IV236_001186"
     CDS             8011..8907
                     /locus_tag="IV236_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8771395.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8910..9623
                     /locus_tag="IV236_001187"
     CDS             8910..9623
                     /locus_tag="IV236_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8771396.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9643..10161
                     /gene="msrA"
                     /locus_tag="IV236_001188"
     CDS             9643..10161
                     /gene="msrA"
                     /locus_tag="IV236_001188"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8771397.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10158..10385
                     /locus_tag="IV236_001189"
     CDS             10158..10385
                     /locus_tag="IV236_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8771398.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10537..11088
                     /gene="lepB"
                     /locus_tag="IV236_001190"
     CDS             10537..11088
                     /gene="lepB"
                     /locus_tag="IV236_001190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8771399.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11171..11713)
                     /locus_tag="IV236_001191"
     CDS             complement(11171..11713)
                     /locus_tag="IV236_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8771400.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11950..12348)
                     /locus_tag="IV236_001192"
     CDS             complement(11950..12348)
                     /locus_tag="IV236_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8771401.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12608..13468
                     /gene="ylqF"
                     /locus_tag="IV236_001193"
     CDS             12608..13468
                     /gene="ylqF"
                     /locus_tag="IV236_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8771402.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13461..14228
                     /locus_tag="IV236_001194"
     CDS             13461..14228
                     /locus_tag="IV236_001194"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8771403.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14284..15141
                     /gene="dprA"
                     /locus_tag="IV236_001195"
     CDS             14284..15141
                     /gene="dprA"
                     /locus_tag="IV236_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8771404.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15263..17341
                     /gene="topA"
                     /locus_tag="IV236_001196"
     CDS             15263..17341
                     /gene="topA"
                     /locus_tag="IV236_001196"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8771405.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17310..18686
                     /gene="trmFO"
                     /locus_tag="IV236_001197"
     CDS             17310..18686
                     /gene="trmFO"
                     /locus_tag="IV236_001197"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8771406.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6517 a         3228 c         3897 g         5087 t
ORIGIN      
        1 gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc
       61 ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact atttggaata
      121 gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga
      181 aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct
      241 ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc
      301 tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt
      361 atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt
      421 gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa
      481 aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat
      541 ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca
      601 ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga
      661 cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac
      721 cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga
      781 caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt
      841 tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag
      901 gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg
      961 cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc
     1021 agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc
     1081 tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa
     1141 aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg
     1201 aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca
     1261 ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg
     1321 atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg
     1381 cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag
     1441 acggtttata tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt
     1501 ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt
     1561 tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca
     1621 gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt
     1681 ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg
     1741 tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga
     1801 ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa
     1861 aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt
     1921 acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt
     1981 tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg
     2041 gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt
     2101 tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc
     2161 cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata
     2221 tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata
     2281 acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg
     2341 aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc
     2401 tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg
     2461 aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt
     2521 tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag
     2581 caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag ttttataaaa
     2641 aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga acacaaaaag
     2701 aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat atccaagata
     2761 aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt atggtgaaaa
     2821 agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg
     2881 gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg atagcagcga
     2941 cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc tttctcaagc
     3001 agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa acagcccctt
     3061 gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa acggagaaga
     3121 ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag aagatgcttg
     3181 gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg aattagataa
     3241 aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat
     3301 tgatgaaccc gatttactat tactagacga accaacaaac catttagatt atgaagcgat
     3361 cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg ttacccacga
     3421 tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg gaaaattata
     3481 cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga
     3541 agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt ggatgagggc
     3601 tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg aagatctaaa
     3661 ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag cgacacaacg
     3721 tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg accaaacact
     3781 gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa
     3841 tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc tggatagcgg
     3901 tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa atgaagaaat
     3961 ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag caaaaacagc
     4021 agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc caagatttat
     4081 gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat ttttgttgaa
     4141 actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg atttggacat
     4201 agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg tgatcactgt
     4261 ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat tccaaggaaa
     4321 cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc aaaaagagac
     4381 ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa
     4441 gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact
     4501 tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata atttcactcg
     4561 attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag aaaaaatggc
     4621 acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat ggaacaagcg
     4681 tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga cagaacaggc
     4741 acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa agggtttcct
     4801 ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct ttggttttta
     4861 aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg ggatgaatgg
     4921 gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac tgattttggc
     4981 catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag caagcttttt
     5041 tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct tggacacatc
     5101 tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa
     5161 caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt ttctgcatgg
     5221 aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt tcaattttat
     5281 gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt gtttctaggc
     5341 gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca tgaaacaggt
     5401 ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat caatcatatc
     5461 gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt agttttgaat
     5521 caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga aggatatgac
     5581 ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg agaaaatgtt
     5641 agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc gtcttccttg
     5701 gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata cactagtgat
     5761 gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt
     5821 tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga
     5881 cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag ggtcagctgt
     5941 ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc atcatgtatt
     6001 cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga tcaatactag
     6061 tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa
     6121 tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct atggatagac
     6181 tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag taaagaataa
     6241 aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa caacatgcat
     6301 atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa gtccgcctgc
     6361 taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag cagccacgac
     6421 acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc tctcccgttt
     6481 atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga ttaatacaaa
     6541 taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat aacttcctgc
     6601 aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg cttttgtaaa
     6661 aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg atccatagat
     6721 agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta aaagaaccac
     6781 tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa acacttcatt
     6841 taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc ctttactagg
     6901 tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt gtcttacatg
     6961 tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata atgacaaatg
     7021 taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat gctttaggca
     7081 ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat gatgaccaac
     7141 tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc aaaacaagcc
     7201 aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac ggcagtgaag
     7261 tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca agacaagcca
     7321 gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac caaggattat
     7381 cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta gagaaaatcc
     7441 ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata tccacactag
     7501 ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct agtgttttga
     7561 acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact aaggggcgag
     7621 gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa gaattgccaa
     7681 atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa gaattcaaag
     7741 agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg aatccggtta
     7801 tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat tagtcgaaaa
     7861 gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt ataaaattag
     7921 gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt ttgataacat
     7981 tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca tttgatcctc
     8041 gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc ttctataccg
     8101 aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa taaaaaagaa
     8161 aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga tcaaaccaag
     8221 caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa tttgaccgcg
     8281 gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt aaagcgagta
     8341 aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac actgactgtt
     8401 ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac ggtcaattcc
     8461 tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga agaatttcga
     8521 tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt ttatttgaac
     8581 tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg aacagaaacg
     8641 gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct ttatcaagga
     8701 ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag cacgacagaa
     8761 aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca tccaaataat
     8821 ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac acaaaaccaa
     8881 tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta tcttcaaaca
     8941 aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg gtagtacttg
     9001 gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa ctcccagaaa
     9061 taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca aataaagaaa
     9121 aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt gatattgaat
     9181 ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta ctaggttttc
     9241 ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat gtccaactaa
     9301 aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg atgaatatga
     9361 tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat ctgactatca
     9421 tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca gataaaatcg
     9481 acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc tctgatgctt
     9541 caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag acaaaagaaa
     9601 ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg agcaatattt
     9661 gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg tatccataca
     9721 gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca agtaaaaagc
     9781 caaacgactg gacatacaga agcagtggag attatctatg atccaacatt gatttcatat
     9841 gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt cggacaattc
     9901 gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga acagcggatc
     9961 attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca gccaatcgtt
    10021 acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca agagtattat
    10081 aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt tatcgaagaa
    10141 aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca ctgcgaggcg
    10201 gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac atccaatttc
    10261 ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat gtcgactatt
    10321 tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat aatcatgaca
    10381 tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata gcctaattga
    10441 aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc ttataataaa
    10501 gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca acgctatatt
    10561 aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc gttcatgttg
    10621 cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa cgtgttgaag
    10681 caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga tattgttgtt
    10741 tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc aggagaaaat
    10801 gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga accttatcta
    10861 actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt tacactacaa
    10921 gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg agataatcga
    10981 agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat tctaggtaaa
    11041 gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga aacaataaag
    11101 cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt tactacctta
    11161 gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt ttgataaatg
    11221 aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat ctacctagaa
    11281 aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg ataaaaaggc
    11341 tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt atctttttat
    11401 gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga aaagcagctt
    11461 gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt tcaagcaaac
    11521 cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat ccgaatgtga
    11581 tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg gctaataaaa
    11641 gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg tcttttgcaa
    11701 tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata ccataaaatc
    11761 accaatgtgt catgacacat tggtgattta catacactgg catctaacaa ttaaaaaaag
    11821 ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa aaaaatagag
    11881 gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa cgtcagatta
    11941 gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta gatcttggac
    12001 ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga aagtaccacc
    12061 attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa cactaaacga
    12121 aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta taaatttttt
    12181 ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca ttgaaccagc
    12241 ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa aagcaacgga
    12301 acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt gtttcgtctc
    12361 ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa atttagattc
    12421 atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt gatagaatga
    12481 ggactggttc actttcactt aactaataga tggaatttct ttataataaa aaagtgaatt
    12541 tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga aaggaatgga
    12601 gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac gagaagtttc
    12661 tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat tacctttatc
    12721 ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg ttttattgaa
    12781 taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt ttcgtaaaca
    12841 aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag gtctagtacc
    12901 tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg ggatcaagcc
    12961 tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta cgttgatgaa
    13021 tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa caaaaggtca
    13081 gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga ttttatggcc
    13141 caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga tcaaagacca
    13201 gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc gatattattc
    13261 aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat tggcaccgga
    13321 attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc gtggcagcga
    13381 attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg atcgttgcga
    13441 agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa cgattgaaaa
    13501 gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca cgcaaaggcg
    13561 tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta attaaggaaa
    13621 aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac caatggattg
    13681 ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca gctgcagtta
    13741 tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta tcagagaaaa
    13801 agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg atcgggatcg
    13861 ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta gcgatgatcc
    13921 aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg atgaccttgg
    13981 atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg tcgattgctg
    14041 ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat gacgtgcttt
    14101 atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac ttagaaggac
    14161 ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta aaaaaatatt
    14221 tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa aggcggtttt
    14281 atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg aatcagtagt
    14341 caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga aatcggattt
    14401 gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg caaaagttgg
    14461 caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat cacttgcttt
    14521 gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt actgttttat
    14581 aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg aagacacact
    14641 tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga agctggatat
    14701 gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc agcaatctct
    14761 catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta tcctgctgaa
    14821 aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga atatcctcct
    14881 caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc agctttgtct
    14941 aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac agcacagcaa
    15001 gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac agaacattct
    15061 accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg tgaagatatc
    15121 ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac aaacgttttc
    15181 tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag gaacacgaca
    15241 atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag aatcacctgc
    15301 aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg ccagtgttgg
    15361 ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca attatgaacc
    15421 ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag ccgcagcaaa
    15481 aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag cgattgcttg
    15541 gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg tcttcaatga
    15601 aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg atgtagattt
    15661 agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt cgatcagtcc
    15721 tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat ctgtcgcttt
    15781 aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag aatattggag
    15841 catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct ggggaatcga
    15901 cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg ctagaattga
    15961 tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa accctgcgaa
    16021 tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact ttcgaacaag
    16081 aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta aacaaggaac
    16141 agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata cagcaaaagc
    16201 agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac atgatcaccg
    16261 aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc caacgagtgt
    16321 gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga aactttatac
    16381 attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag atacaatgaa
    16441 agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa tcaaattcaa
    16501 aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga aagaaaatat
    16561 tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac caaaacaaca
    16621 ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac tagaagaaaa
    16681 cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa gacgctacta
    16741 tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg tcaattcatt
    16801 aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa tggaaggaga
    16861 tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc gtttctataa
    16921 accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc aaatcaaaga
    16981 tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa aattagggaa
    17041 atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga aaccaatcgt
    17101 aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg aacgaaaatc
    17161 taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat tcacttcttg
    17221 ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg agaagaaagt
    17281 caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca tccaaaaata
    17341 atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg cttgccggaa
    17401 gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat gaaatgcgtc
    17461 cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta tgttccaatt
    17521 cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa atgcgccgat
    17581 tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt ggtgccttag
    17641 ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag catccattag
    17701 taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta gtagcaacag
    17761 gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt tcagatggtt
    17821 tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat atggataaag
    17881 tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt ccgatgacag
    17941 aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca gaacttaaat
    18001 cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg gcacagcgag
    18061 gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat cctaaaactg
    18121 gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct tctttataca
    18181 acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt ttccggatga
    18241 ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga aacagcttta
    18301 tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa gatctgttct
    18361 ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc ggactacttg
    18421 ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt ccacaagaaa
    18481 cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat ttccaaccga
    18541 tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac aaaaaagcaa
    18601 gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa acagaattag
    18661 aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga cggatagtct
    18721 cgctctttt
//
LOCUS       DACDID010000044        18585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDID010000044 DACDID010000000
VERSION     DACDID010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.044"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(179..559)
                     /gene="yajC"
                     /locus_tag="IV236_001198"
     CDS             complement(179..559)
                     /gene="yajC"
                     /locus_tag="IV236_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8771407.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(656..1801)
                     /gene="tgt"
                     /locus_tag="IV236_001199"
     CDS             complement(656..1801)
                     /gene="tgt"
                     /locus_tag="IV236_001199"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8771408.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2019..2717
                     /locus_tag="IV236_001200"
     CDS             2019..2717
                     /locus_tag="IV236_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8771409.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2885..5524)
                     /locus_tag="IV236_001201"
     CDS             complement(2885..5524)
                     /locus_tag="IV236_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8771410.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6143..7174)
                     /gene="queA"
                     /locus_tag="IV236_001202"
     CDS             complement(6143..7174)
                     /gene="queA"
                     /locus_tag="IV236_001202"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8771411.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7367..8542
                     /locus_tag="IV236_001203"
     CDS             7367..8542
                     /locus_tag="IV236_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8771412.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8546..9187
                     /locus_tag="IV236_001204"
     CDS             8546..9187
                     /locus_tag="IV236_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771413.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9184..10101
                     /locus_tag="IV236_001205"
     CDS             9184..10101
                     /locus_tag="IV236_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8771414.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10098..10769
                     /locus_tag="IV236_001206"
     CDS             10098..10769
                     /locus_tag="IV236_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771415.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11310..11798)
                     /locus_tag="IV236_001207"
     CDS             complement(11310..11798)
                     /locus_tag="IV236_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8771416.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11999..12265)
                     /locus_tag="IV236_001208"
     CDS             complement(11999..12265)
                     /locus_tag="IV236_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8771417.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12265..12528)
                     /locus_tag="IV236_001209"
     CDS             complement(12265..12528)
                     /locus_tag="IV236_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8771418.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12521..12793)
                     /locus_tag="IV236_001210"
     CDS             complement(12521..12793)
                     /locus_tag="IV236_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771419.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            13004..13189
                     /locus_tag="IV236_001211"
     CDS             13004..13189
                     /locus_tag="IV236_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8771420.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13331..15154)
                     /locus_tag="IV236_001212"
     CDS             complement(13331..15154)
                     /locus_tag="IV236_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8771421.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15752..18457)
                     /locus_tag="IV236_001213"
     CDS             complement(15752..18457)
                     /locus_tag="IV236_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8771422.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         5300 a         3931 c         3478 g         5876 t
ORIGIN      
        1 tttatttaca ggtgtttgta tagaaaaata tataaagtta tttattatta tttatatgac
       61 acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa
      121 taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt
      181 atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa
      241 ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag
      301 tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt
      361 ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga
      421 tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt
      481 gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa
      541 gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt
      601 agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga
      661 agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa
      721 gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata
      781 aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga
      841 tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga
      901 aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc
      961 catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac
     1021 catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga
     1081 tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata
     1141 acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac
     1201 gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag
     1261 catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt
     1321 agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca
     1381 atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt
     1441 gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt
     1501 gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc
     1561 cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa
     1621 taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg
     1681 ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta
     1741 aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca
     1801 ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca
     1861 agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc
     1921 ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat
     1981 ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa
     2041 aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa
     2101 tccctacatt gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg
     2161 ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta
     2221 gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta
     2281 tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag
     2341 atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga
     2401 tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct
     2461 attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac
     2521 ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac
     2581 ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca actgctggta
     2641 ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat
     2701 tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc
     2761 gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac
     2821 attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag
     2881 ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg
     2941 cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta
     3001 ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca
     3061 gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc
     3121 tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag
     3181 tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta
     3241 aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca
     3301 ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt
     3361 gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa
     3421 acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa
     3481 taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat
     3541 aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa
     3601 tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt
     3661 tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac
     3721 ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga
     3781 cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc
     3841 ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag
     3901 tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga
     3961 ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta
     4021 agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac
     4081 caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt
     4141 ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga
     4201 tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa
     4261 gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa
     4321 cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt
     4381 gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg
     4441 tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca
     4501 tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg
     4561 tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt
     4621 atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg
     4681 aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata
     4741 ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga
     4801 cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt
     4861 ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc
     4921 ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg
     4981 catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg
     5041 atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat
     5101 cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg
     5161 ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga
     5221 caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat
     5281 ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga
     5341 tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct
     5401 catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc
     5461 cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt
     5521 ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat
     5581 ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat
     5641 ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg
     5701 aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg
     5761 ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg
     5821 cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt
     5881 gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg
     5941 taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg
     6001 atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc
     6061 ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca
     6121 aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac
     6181 gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg
     6241 cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta
     6301 ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga
     6361 tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag
     6421 ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca
     6481 aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga
     6541 atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct
     6601 catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt
     6661 ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag
     6721 gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt
     6781 caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac
     6841 caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg
     6901 tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt
     6961 ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct
     7021 gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca
     7081 aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg
     7141 gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc
     7201 ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt
     7261 ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac
     7321 tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca
     7381 aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt
     7441 tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat
     7501 gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc
     7561 catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat
     7621 tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa
     7681 agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc
     7741 ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg
     7801 agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc
     7861 gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt
     7921 gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg
     7981 tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg
     8041 tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa
     8101 gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga
     8161 aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt
     8221 taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg
     8281 aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc
     8341 cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt
     8401 cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt
     8461 ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc
     8521 tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat
     8581 tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat
     8641 cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat
     8701 tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc
     8761 gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc
     8821 gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc
     8881 aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat
     8941 gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact
     9001 tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta
     9061 ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga
     9121 ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga
     9181 agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt
     9241 tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga
     9301 atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca
     9361 gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg
     9421 atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca
     9481 ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt
     9541 gagaaaagat acggtcaaac gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt
     9601 cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat
     9661 gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt
     9721 tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt
     9781 ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg
     9841 gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca
     9901 ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa
     9961 gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac
    10021 gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac
    10081 cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct
    10141 attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt
    10201 ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta
    10261 ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt
    10321 ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg
    10381 ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc
    10441 aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac
    10501 tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt
    10561 tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag
    10621 atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat
    10681 tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc
    10741 tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac
    10801 catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg
    10861 attcttccct ccttatgggt aaagagttgc ttttcttagc tatttatcca ttccgtatac
    10921 tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat
    10981 ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc ataccacatg
    11041 atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc
    11101 gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc
    11161 atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt
    11221 gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt
    11281 ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg
    11341 gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc
    11401 caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata
    11461 ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg
    11521 ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc
    11581 gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac
    11641 tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca
    11701 tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact
    11761 aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca
    11821 taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata
    11881 ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag
    11941 agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc
    12001 actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt
    12061 aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca
    12121 gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt
    12181 caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt
    12241 tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt
    12301 aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag
    12361 aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc
    12421 actacgatca ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg
    12481 ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc
    12541 tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac
    12601 ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg
    12661 atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa
    12721 ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac
    12781 aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct
    12841 gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt
    12901 ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt
    12961 gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg
    13021 taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt
    13081 aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg
    13141 attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat
    13201 aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag
    13261 aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt
    13321 aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc
    13381 aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc
    13441 tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt
    13501 tacttacgat atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga
    13561 cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt
    13621 tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg
    13681 cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat
    13741 tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt
    13801 tataatgatc atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag
    13861 gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg
    13921 cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt ttccatttga
    13981 cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa
    14041 tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg
    14101 tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga
    14161 atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag
    14221 caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa
    14281 agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc
    14341 aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca
    14401 atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg
    14461 cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag
    14521 aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca
    14581 cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg
    14641 gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca
    14701 aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga
    14761 tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag
    14821 agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc
    14881 ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata
    14941 aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga
    15001 ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca
    15061 ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag
    15121 gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt
    15181 tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta cgctcactga
    15241 ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa tatcaagcgc
    15301 tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt tttctttgaa
    15361 ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt tttgatatct
    15421 taacttttgg aagagagatg taaggctgct ttctacccat cattcttact actcgaagca
    15481 tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc tgcggtaata
    15541 agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt attacttgat
    15601 gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga ccctcggtat
    15661 tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct ctaatactca
    15721 ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc tcatgcgccg
    15781 cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt cctacgattc
    15841 caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt ggccaataaa
    15901 ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag aacggattga
    15961 tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg ccagagatcg
    16021 acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat gcagccaaga
    16081 catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg ctgtagaccg
    16141 gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc aagctttgcg
    16201 caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat
    16261 tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa agaagaacag
    16321 cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg ttatttgcga
    16381 ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca ctttcttttg
    16441 ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt tcgccttttc
    16501 ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca ttgttgatca
    16561 agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt actgttgtca
    16621 atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa tcagcggcca
    16681 atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc ccttcattga
    16741 tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca tggatttctt
    16801 gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca ttcgttacag
    16861 ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc
    16921 cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca gtggaactga
    16981 tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg gcatcaattt
    17041 gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg ttcaaagcag
    17101 ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga tcaataattg
    17161 gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg accagtttat
    17221 cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca ttgctgatgt
    17281 ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca gtatctatta
    17341 acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct acactgtttg
    17401 tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg tttgctagat
    17461 cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact tgttggatga
    17521 tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg atcgaagcaa
    17581 aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg attttttctt
    17641 gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca ttcaatgcaa
    17701 cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca tttgctttat
    17761 tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt tttgtgtagc
    17821 cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact ttattgatac
    17881 ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat aaagtcgaac
    17941 tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct cctttatcgg
    18001 tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt tttggtttct
    18061 tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc aagtaaatac
    18121 ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa tcaacgaatt
    18181 gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca atggccactt
    18241 ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc agctctccag
    18301 tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc gaagggatga
    18361 tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa
    18421 gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt cttctttttt
    18481 cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt tcaaaaaagt
    18541 cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctctt
//
LOCUS       DACDID010000045        18450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDID010000045 DACDID010000000
VERSION     DACDID010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.045"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV236_001214"
     CDS             complement(109..564)
                     /locus_tag="IV236_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771423.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV236_001215"
     CDS             complement(718..1926)
                     /locus_tag="IV236_001215"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8771424.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV236_001216"
     CDS             complement(2038..2370)
                     /locus_tag="IV236_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8771425.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV236_001217"
     CDS             2518..3393
                     /locus_tag="IV236_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8771426.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IV236_001218"
     CDS             complement(3475..4056)
                     /locus_tag="IV236_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8771427.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IV236_001219"
     CDS             complement(4062..5321)
                     /locus_tag="IV236_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8771428.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IV236_001220"
     CDS             complement(5323..6369)
                     /locus_tag="IV236_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771429.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IV236_001221"
     CDS             complement(6604..6879)
                     /locus_tag="IV236_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8771430.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IV236_001222"
     CDS             complement(7122..8432)
                     /locus_tag="IV236_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8771431.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV236_001223"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV236_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8771432.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IV236_001224"
     CDS             complement(9978..10658)
                     /locus_tag="IV236_001224"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8771433.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IV236_001225"
     CDS             complement(10707..11342)
                     /locus_tag="IV236_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8771434.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IV236_001226"
     CDS             complement(11408..12811)
                     /locus_tag="IV236_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8771435.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IV236_001227"
     CDS             complement(12798..13829)
                     /locus_tag="IV236_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771436.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IV236_001228"
     CDS             13860..14081
                     /locus_tag="IV236_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8771437.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IV236_001229"
     CDS             complement(14246..14983)
                     /locus_tag="IV236_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8771438.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IV236_001230"
     CDS             complement(15136..16476)
                     /locus_tag="IV236_001230"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8771439.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IV236_001231"
     CDS             complement(16713..18236)
                     /locus_tag="IV236_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8771440.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5196 a         3931 c         3111 g         6212 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg
//
LOCUS       DACDID010000046        18415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDID010000046 DACDID010000000
VERSION     DACDID010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.046"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(237..1529)
                     /locus_tag="IV236_001232"
     CDS             complement(237..1529)
                     /locus_tag="IV236_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8771441.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1526..2149)
                     /locus_tag="IV236_001233"
     CDS             complement(1526..2149)
                     /locus_tag="IV236_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771442.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2152..2988)
                     /locus_tag="IV236_001234"
     CDS             complement(2152..2988)
                     /locus_tag="IV236_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771443.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3005..3892)
                     /locus_tag="IV236_001235"
     CDS             complement(3005..3892)
                     /locus_tag="IV236_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771444.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3900..5135)
                     /locus_tag="IV236_001236"
                     /pseudo
     CDS             complement(3900..5135)
                     /locus_tag="IV236_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5818..6501)
                     /locus_tag="IV236_001237"
     CDS             complement(5818..6501)
                     /locus_tag="IV236_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8771445.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6519..7136)
                     /locus_tag="IV236_001238"
     CDS             complement(6519..7136)
                     /locus_tag="IV236_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8771446.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7146..7970)
                     /locus_tag="IV236_001239"
     CDS             complement(7146..7970)
                     /locus_tag="IV236_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8771447.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7963..8523)
                     /locus_tag="IV236_001240"
     CDS             complement(7963..8523)
                     /locus_tag="IV236_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771448.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8697..9653
                     /locus_tag="IV236_001241"
     CDS             8697..9653
                     /locus_tag="IV236_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8771449.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9958..10914)
                     /locus_tag="IV236_001242"
     CDS             complement(9958..10914)
                     /locus_tag="IV236_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8771450.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11018..11620
                     /locus_tag="IV236_001243"
     CDS             11018..11620
                     /locus_tag="IV236_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8771451.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11708..13207)
                     /locus_tag="IV236_001244"
     CDS             complement(11708..13207)
                     /locus_tag="IV236_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8771452.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13223..13747)
                     /locus_tag="IV236_001245"
     CDS             complement(13223..13747)
                     /locus_tag="IV236_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771453.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14045..14794
                     /locus_tag="IV236_001246"
     CDS             14045..14794
                     /locus_tag="IV236_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8771454.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14785..15570
                     /locus_tag="IV236_001247"
     CDS             14785..15570
                     /locus_tag="IV236_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8771455.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15539..16255
                     /locus_tag="IV236_001248"
     CDS             15539..16255
                     /locus_tag="IV236_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8771456.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16357..17376)
                     /locus_tag="IV236_001249"
     CDS             complement(16357..17376)
                     /locus_tag="IV236_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8771457.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17395..18414)
                     /locus_tag="IV236_001250"
     CDS             complement(17395..18414)
                     /locus_tag="IV236_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8771458.1"
                     /translation="MSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALP
                     ALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIF
                     GSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVN
                     PIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSS
                     PLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFE
                     ALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHG
                     IGLFS"
BASE COUNT         5508 a         3879 c         3242 g         5786 t
ORIGIN      
        1 ggtttactta tttttttgta aaatctaatg atttttaatg cattattcct ttttgttcgg
       61 gtttaaatag ttatttttat tttctcctat ctaaaaaagc agcccacata aaaagaaggc
      121 atgacaaaaa gcatgtcata gccccttttt ccgaataaat agtgttcaaa agacagccta
      181 tcggcaataa ggcaagattt tataaactac gggaagtaat ttccgtaaat ctattcttat
      241 tcttttaagc tgacaagctc gtcaatcact actgcttttc ctgaagcaat agaatgattt
      301 gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt gagttgctgc ccgattccac
      361 ggatcttctt ctttttcttc accaaataga tcatctaata aacgcggatc tcctccgcca
      421 tgtccgcccg cttcttgttc gacgattacc tcatatggct caccgaacat cggatagaca
      481 tggatggact gaagaacggc tgctccttca tcctttttat ctccacctgc attgatgtaa
      541 gacttttcga tcactttcat ttcgatccgt cctttgctgc cattgaaaga aacattgaat
      601 ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa tcgcaccagt ttcatatttg
      661 accgttacgc ctagtgtatc ttcaatagag atatcatcgg aaaatacact gcggtcacga
      721 atatatccac tgtctttttc tgcttctaaa tacatttctc gcaattcctc attggctgac
      781 aggtctatag caaatggatc agctttagct agttcgcttc catagcatcg actgtaaaat
      841 atttctactc cgcgtttctc cgcattatgc atcccataaa agtttaatcc accgaatgca
      901 aatacctttt ggggacggct gttcaaccaa aaattcacta gatcaaaatg atgggtagat
      961 ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat gccatcttct gaaataatct
     1021 gccccgtgtt gcgtatttaa cagccattcg aagtggatag aaaacacatc accaatcaca
     1081 tcactttcta tcagttcctt tatcttcgtg tgatacggtg cataacgata attaaaagtg
     1141 actcgcaggc ttcttcccgt ctgtttttgg accgttatta tttcttgagc tttttcctca
     1201 tcgatagtca gaggtttttc gcaaataaca tcatatcctt ttttcattgc acgaatgata
     1261 taatcatgat gcgttcgatc tactgacgta ataatgactt cagccggccg tacgtcatcc
     1321 agtagttgct caaattgtga tggcatacat gtctttactt ttttctgtcc atacttgttc
     1381 acaagtatcc gatttgcata atccatcctt gtttgagata catcacaaaa accgactagt
     1441 tcacaagttt gatgatacgt agaggcaatt gcctcataaa aaaaacgcgc tctcccacca
     1501 agtccgactt gtacatattt tttcttcatt taaagtcatt ctccttttct tttaagcaaa
     1561 atactgtgac aaatggcagc cctggataca gtaatgcagt gagtaaaaaa tcaggctgta
     1621 tacaccagaa cagtccatac aaaaggcata aataccacag aaaccccttg acgttttttg
     1681 acaacagcca gatattcctt ttgatcaaat aaacaagact atctgcttta tcttctttgc
     1741 agatatcaat tagcgtacta aaaaaactat gggatacaaa aagcaacaga cacaatagaa
     1801 aaggaatcag ataccaagct ccaatctttc ccactaataa ataccaatca actgcaaaaa
     1861 taatgacaaa cagctgaaac aacgtaatga tgaaacacat ccgtttgatt ttccctttcc
     1921 tccaacatac ctttttttct gccaagtaat ggacagatag ataataagaa ggatacatca
     1981 tcaaaaaata aatcagatag acaacacgag cagacagcac taatcctaaa gtgaacaaac
     2041 atcctagaac taaagcgaaa agagcattac tgataagact ggttaaaaaa atccgacaga
     2101 catagtagtt gagcttgtaa agaaacatct cttctattgg ctttttcata ttcagccctt
     2161 cagaccgctt gtactgatcc cctggacaag atatttattg aagaataaga aaatcagaaa
     2221 aaccggtagc agcgacaacg tagacatcgc aaacatactc gcgtaagtcg tactagatcc
     2281 agcgtctgca aataatttga tggcaatcga taccgtgtaa gatctcggct tgtttaaata
     2341 aatcaatggt cccatgaagt cgtcccattt ccaataaaac tggatgatga tcgtcgtgat
     2401 gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag acactatact tgctgcatcc
     2461 gtcaataatc gctgattcat ctaactcttt ggggatgctg accatgaatt gcatgatctg
     2521 gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc agcggcacat acgtcccaac
     2581 caaattcagc cgattgtaaa taataaactg cggaatcaaa atcacttgtg caggaatcat
     2641 catcgttact aacatgacga cgaaccagaa tctttttcct ttgaaatcga ttctcgataa
     2701 tgcgtaagca atacaagtcg agctcagaac tgtccctatc gtcgaaactg cagagatgac
     2761 aaacgaattc ctaaagaaag taaaaaatga aattccagca aaccccgccc atccttttgc
     2821 gtaattttcc cattgaaaag tctctggaat caaattcagc gaaccactca taatctcttg
     2881 atccgtttta aaagaacctg cgatcatcca taagaccgga taaatcatga ctagacctaa
     2941 tagcaaaata aaaccgtatt gaaaccattt cgttactgta ttattcattt cttcctcctc
     3001 tcattcacac aacgacccat ttttttgatg atttgaaaat cagaaaggtg acagctccca
     3061 taatcaacag catgatccac gaaatagcac tagcataccc catagaatta aaagaaaaag
     3121 cttggttata cacataaaga gcataaaagt tcgtggcatc attcggacca ccttgggtaa
     3181 tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt ctgcataacc aagttataca
     3241 aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa cgtctgccat ttgttcgctc
     3301 catcgatctc tgccgcttca taatacgagc ctgggatctc tttgatacct gcagcaaaga
     3361 tgatcattgc cgaaccgaat tgccaagcag acatcaaaac aagagggatc atgaccagtt
     3421 tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg ttccaggaaa atattcacta
     3481 acccgttttt agaaaacaac tcccgccaaa ctagagcgat agaaatactg ccaccgatca
     3541 aactaggcac ataatacaaa gaacgaaaga aagtcgttcc tttgatattc ctcgttaaaa
     3601 gataagcaat caatagacca aagatcagtt tcacaggtac agatgcaaat acatagagaa
     3661 aagtgactcg gatggagttc aaaaaacggg catcactcag catctttata taattatcca
     3721 agccgatcca tgagactgga tttcccaaac gataatcggt gaacgaataa taaagtgaca
     3781 agatcatcgg tatcactgtt atcaatagaa atcctaggat gaatggagaa gcaaatacgt
     3841 aaccgaccac attgtcttga tccaagaacc gttttagttt ttcttttttc atgccttcct
     3901 tctgctgtta cttcttcata aatgactttt tcaactaaaa gattgtattg gtcttgaatt
     3961 tccggattca aagcactatc aaagacattc acttcgccgt tgtccatatc ggaaatcatt
     4021 tctaaataat caaatgtcgc ttgcaaagtt gcatcatctg cacgagcgag ctgttctcga
     4081 atattcgaca tgattggtac accacgttct ccttttaaaa tttcattggc ttcttcactg
     4141 ttttgaaaga aatcaataaa cttagcagct tcttctttat gggctgagct ttttgccata
     4201 ctcagcattt gcgaagaacg taaagggata ccagattctc catctgcttt tctgcgaggt
     4261 ggattaatca actgaatctc catatcttca ggagcccctg acaatatttc tgtcaactgg
     4321 ttacttgata gaaacaccat tgccgcttct ctatccctaa ccgcctgaaa agacagatcc
     4381 gtcgtacttg cagctccacc acctggttct ggatgggccc ctttgtctac cagattttta
     4441 cgcagctgga aaaaatctgt tagatagtct ggattttcaa atcccaagct gttagataga
     4501 tcttcccgat tataaaaatt caatcctttc tcaacttgtg gtatttgaat aatacagcca
     4561 gctataaaat cttcgaatct tgaaatagca tagattccta attcttcagt gatttgatag
     4621 ctgatttgtt caaattcatc ccatgtccaa tttgaagctg gttcagggat atttaattgt
     4681 tcaaatatat ttttattgta agcaatcgca aacgaattca ccccgttaga cagtccaact
     4741 aattgcccgt cgtaagtcgt cgttgctaaa taggattcat ctgcattaga aatatcaatt
     4801 atcccttgct ccacatattc atctaatggt tcaatctgat tgatgtattg aggaaattga
     4861 ttgttcagtt ggaaaatgtc ccagatatca tctgatgctg ctagtgtacc taattttgtg
     4921 taatatcctt cataatcata aaattcataa tcgatcgtca catggggatt tttttcttca
     4981 taaagctcga ttacttttac tgttgcatcg tgtcggactt gcgaacccca ccaagccatg
     5041 ttcagcgtga tttttttgtc atcttcttgc ccacaagccg tcaagaagaa taacataaca
     5101 cttaaagcta agatgattct cattcttttc ttcattctct gcacctctac ttccattgtt
     5161 atttttcaat atactgtatt tttatacaga acatatttat taaacgatct ttttcaaggg
     5221 gaataaaaac caagacttgc cttatcgttc tgcctaggat gaacaactca atactttcaa
     5281 gaaaaattag atagtgctga ttcttctttc atcgctaaaa acacttcttg cttcaatgat
     5341 gggaggataa ttacgcaaag aaaaaaggat atcagacaac tgcccaatat ccttgacata
     5401 gctaaataag catgcttctt cgtgtgcgct cttccctaaa aaacagaaaa ataactagta
     5461 attctgtatg aaattaggta caatatacac gtcgatgcac aagcattgac tatggcaact
     5521 tgattggtca gttcccgctg acttttcaag ccccgacaat ttagctgcta ctaaattgtc
     5581 gggtgccttt tttagttttt attcgttttt cattctatag aaagcgcttc aataaatcaa
     5641 gacatttttt tgttagaatg ctaataataa caaaaggttt taactcttct tatgtacatt
     5701 acaggtaaaa aaacaaaaaa atttctcttt tttttgaatt ctgaacaaat agtactggag
     5761 tacaaaagac gaggaagagc cactatctgc tcttcctcgt cttttgattc tcacctgtta
     5821 ttcatgattc tttttctcag tttcccgata tttttccatt ttacgactaa aaagctctcc
     5881 gctacttggt ggcgtatatg tgatggcatt tgctcctgct tgaatggtct cagcaatact
     5941 ttcttccgtt ggaccacctg tcgcaatgat aggaagttca ggatattttt tacgaaaatg
     6001 gcgtactgct tctgcagttt ctttacctgc actgatattg ataatcgaaa taccagctgc
     6061 taacttttca tctatttctg tatatttcga cgttaccgta ctgataactg gaatatcaac
     6121 tactcgacaa acttctttta ctgtctctaa aggcgtagga ccgttcaaaa cgacccctaa
     6181 cgaaccttgc gcttctgcga acatgctcat gtaggaagaa cgagcacctt gtgtcaaacc
     6241 accacctaca ccggagaaaa ctggaatgtc agctgcctca ataatgcttt tagtaatcgc
     6301 tggatgaggt gtaaaaggat acacagcaat tactgcattc gcatttgtat tacgtatgat
     6361 cgcaatatcc gtagtaaaga tgatcgaacg gatcaatttt ccaaatatct tgatcccgct
     6421 tgcttctaaa atgatttccg gtactcttac gatatctttt ctcaattccg tcatgatttc
     6481 tggcgtaaat tgttctgcca tcagccactt ctcctttgct atctccggtc atactgattc
     6541 aattgatacg tctcgatgac ctggatgatt ttttgcgcat aaccggggtc tgtcgcataa
     6601 cctgcatctt gcaatgcttg tgcggcttcc tgatagttgg tggccgtaat cactttttca
     6661 tacagcgcag gattccaatc tactccttgg acgaacagtt gtgtatggtc atccatggac
     6721 tgttcccatg aatcatacac tttgaagtct ccttggatag ttatccattc ttcgttaaca
     6781 aattcctttg tttccaggct tactttcttc tgatccccat aagccttgat cccgaaaaga
     6841 ttattgtatt tgctagccaa ggtacttttt ccccaatttg attctaatat agcttgtcct
     6901 aaaatgatac taggaagcac tccataacca tcttgtagtt cttgggcatg cggaacaagc
     6961 cgttcaataa attgctgatg ggattgcgct tcattttctt gctgattatc agtaaaagga
     7021 gaagacagat tcttcaagga aaaaacaaag gctaagagta tcactgctac accggcaacc
     7081 actgctggcc aacggatatt cttgcgcttt tgctttttcc actttttttt cgccatgatc
     7141 aattcctact tttcatttaa ttcattcaga tattcttcta gcgtcaattg gtgtttggtg
     7201 atatactcag cgttttcttt accgacataa cgtaagtgcc aaggttcata tgtgatctta
     7261 gttatatctt cgtgtccttt tggataacga acgataaatc catactgcgc agcattatct
     7321 gcgatccatt tagctccagg ctgatctccg taacttgcat ccaatacagt agaaggataa
     7381 ctgttgtacc agttttgatc gacgacatcg actgccagac ctgtgtggtg ttcgctgtat
     7441 cctggctccg taatcgtttc tttagtaatc tttgtcgcct cctcttctga tacaccttga
     7501 cgactcatca catcttggac attttgttcg aaaacttgtt gttgggatga tacagaccgg
     7561 taagctgaga ccatcactaa tggaaatcca gctgcttcag cagccttggc aaatgcttca
     7621 tattctggag caatgcgttt gtcgatcaag tatccgttac ttaatgatgc caattgagta
     7681 gactcatcaa tttctttttc taatttgtga tctggaccaa ccaaaactag cttccaatcg
     7741 tctgttttaa catctggcaa ttgtttggac gtacgatctt ttgtttcttt gtcctttacc
     7801 ttgctgctgg ttgtttgact gttcttttct ttactagcag ttgttttggt gcttgttcgt
     7861 tcgactgctt gattgccggc ttgaacatgc gaatcatcat cgctaaaaat aacatagccc
     7921 attgctgcga tcataatcac aactgctcca aatcctaaaa atttattcac ttgaaaaatc
     7981 ctttcgttgt tcttcacgtt ccccattcgc ctcatgaacg taagtttctt ccagattttc
     8041 attcacccaa tcgagaagtt cgattttttc tccttcgatc tgtttttgaa catctgctgg
     8101 cagacgaaaa ttcaaaatat catcatagcc ccattcatca tattgaatac ttactttcaa
     8161 gtcaaggtat ctaccagcct gctgattatt tttcaaaggg atcagctcga cttttgctgc
     8221 tttgctgcta agccattttt gcgcgatgcg tgttttgatt tgcttgtact ctgcaacggc
     8281 aacgtttcgt ttttcgggaa aaataatcgt ttgacgcaaa atcttttgcg ttagcatcca
     8341 ttcataatca gtgaaaagat tttttacttt ctgcatcgct tcagatggca gatttttctc
     8401 gccatctttc catttttgcc attgttctgg actgatcagc aagtattcat ctaaaaaatt
     8461 tttttcagtt tcaaactgtt ctaataatac actcataata agatcaatat atacttgatg
     8521 cacgtttatc ccctcctttt aactattcta cttaagtttt gatcaaaaag cacctcttaa
     8581 atcgctgctt tcattttttt taatgtttca agtcctctcc tctttattct tcgtgatttt
     8641 tgttataata atagacagac aagaaactca atccaactat gaaggagcgt tttcagatga
     8701 aaaaatatca atggggaatc gttggtcttg gtaccatcgc acatgagttt gccgaaagct
     8761 tcaatcaaga gacgagcgaa ctaacagcag ttgcttccag aacttcagaa aaagcggaaa
     8821 acttcgctca ccgttacaat attccaaaag cttacggaag ctatcaagaa atgttagacg
     8881 atgctgaaat cgacatcgtg tacattgctg taccaaatag acagcatatc gaccatatct
     8941 tggcagcatt ggaagcaggc aaacacgttc tatgtgaaaa agccatcacg atgaataaaa
     9001 aagaactggc tgacgctatg cgcttagcag aagaaaagaa tttgatctta gcagaagcga
     9061 tgacaatctt caacatgcca ctttatcagc agttacgctc aataatggat acaggaaaac
     9121 tgggggcttt aaaaatgatc caagcacctt tcggcagtta caaagaacca gaccctaaaa
     9181 accggttctt caatccagaa ttagctggag gagctctgtt ggatatcggt acgtatgctg
     9241 tatctttcgc tcgtttcttt ttgagctctc aacctgaagt agttgcctct acaatggttc
     9301 cttttgaaac aggcgtggac gaacaatccg tcactatctt gcgtaacaaa gaaaatgaac
     9361 ttgctgctgt cagcttgact tttcaggcaa aaatgccaaa agtcggtatc gttgcctttg
     9421 agaacggtta tgtgacgatt gctgattacc cgcgtgctga tcgtgcggag atcttattta
     9481 cagatggtac aaaggaattc atcgaaagcg ggaatacatc tgaagcaatg aattacgaaa
     9541 taaagaatat ggtacacatg attgaaggtg acttgcccaa tcgctctctc ttcttgacaa
     9601 aagatgtcat cgaaatcttg gatcaaatgc aagttctatg gaaaaatatg taattttaaa
     9661 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
     9721 cctatttttc gtttggctgg acttattacc gcagaggaaa gcttttgaac accgtttaag
     9781 aaagaggttg tgagcctacc gttcagctcc gagtcacaag gagcaatttc aaaaaacagc
     9841 ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttgacaggag aacaccgttt
     9901 aaagaagagg ttgtgaaatc agctttttga cctgaatatt agctgaattc acaacctcta
     9961 tttttggatt ttttgttggg agaattctgc cttgatttca ataaaacttt taaaagactg
    10021 caaaaactgg aacaattctg ctcgatcctc gaattcaggg cgactagtcg gcagaggcat
    10081 ttgccgatac aatacataaa cttcttcaat acgtgttaaa atttgtctgc catcatttga
    10141 ctctgaaaat gtttctgcag tataaatcaa taaaccatag atatgttttc cataataagc
    10201 tggttgctgg attctctcta gattttccag catgtcttta atcacattgg cctgtgctct
    10261 tctcatagag aagtaagttt cgtagtatag cggctgtctg agccagtagt tttcttgatg
    10321 ttctctggca tcttcttggc tttcgcgaat gaatgtcagt aaatgttcgc atttgatctg
    10381 cacatcttct ttattttctg ataaaagaaa ttctgccatt tgtctcaaaa tatttcgaaa
    10441 ttctttttcg atctgtattt ggttttcctt cagctgcttc gtacgatctg gcatgtaaac
    10501 atttgcaagc aaagcaaata ctaatccaat cgccattaat cccgcttcat tgccgatcag
    10561 ctgccaagac atgttttcct ccactaaata atgagtgacc aaaaccgaat ttactactat
    10621 cccttcggtt aatttgaagc gagaggcaag cgggatgaac aggaataaaa aaagactgaa
    10681 gccaaaaata gtatacccaa cagcaaaaaa actgaacagt gctgttgccg tagctaatat
    10741 aaaggctgcg attcgattca tgcccgtacg caatgtagag cgcttggtat ttcctacact
    10801 taataatgca atgatccctg cagctggcca atacaacagc gataagctac ccgctgtgac
    10861 catcgaaaag aagacactga tcactgtttt gactgttcgc attcctatac gcatttactt
    10921 cccccttttt tattttaatt atacttgaat aatgaaaaac tagctatcag acaaaagttt
    10981 aggtatgata gtaaaaagac caaaaaggag aatctctatg caatttggcc gttttcgact
    11041 aggtatgcgt acgatcaaaa gtgccttagc tgttttttta tgtatcttgt tttttcattt
    11101 gactcaacga ggaatcccga tgattgctgc attatctgct gttttttctt tgcgtcagga
    11161 cttgactaca accgtttctt ttggaaagtc taggatcatc ggcaatacat taggtggtac
    11221 attagctatc atttattttt ttgtcaaaag ctattttcac aatgattttt tggtagaatt
    11281 gatcttgctt cctattctag ttattattgt gatcgtcgtg tcagatggta ttaacaataa
    11341 ttccggtatc atttccgcaa tagctacgtt gttgctgatt gcgttgagca tccctcaagg
    11401 cgaatccgct ttgtatgcta tccaacgtgt actagatact tttataggga cattcattgc
    11461 aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa aagaaggaag aactatcaga
    11521 agatttggtc gaactccaaa agaaagaacg attgctaaga acaaatttac aagagataga
    11581 agctaagatc caaaaacaaa agaaaaatag cgaggactaa ttttcttttg caataaaata
    11641 aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt tttgattgaa cggtgaagtg
    11701 tactacttta ttcttccttc atcgagtttt gttctgcctc ttttggcgct tcatacatat
    11761 ttacttggct agtagtccca cgtgtcgaca aattctctac gatttccttc aagtcaagtc
    11821 ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa atttgtggca taatttgtca
    11881 ctcggttcgc tccactggtt tctcctgaac tgcttcctgt atcgacaaca gagatttttt
    11941 cgatatttcc tagcggctgt gctgcttcgc gcatcaattg cggtagcata tcgataacca
    12001 tactcaagac agctgcttcg ccatattcct tgaaggcatt ggcgatcttt tgttttgctt
    12061 cggcttctgc ttttcctttt gctagtgctg cttcggcttc tgcttgtcca gctaaacgtg
    12121 tcttattggc ttccgcttct gccagtgctt ccacacggaa tcgttctgct tcggcttcag
    12181 tcacttcttt gactttttga gcttgtgctt cttgttcttt agcgtaccga tcggcatctg
    12241 cttttttctt cacttccgaa tcatattgct tttcacgacg tgtgatttct ttttcttcca
    12301 gttcgatttg tttttggcgt tctacgattt taatctgcat ttcctgttcg attacttgtt
    12361 gttgtgctcg ggcactttct aaattgtagg cttggtctgc ttttgcttta gcaacatctt
    12421 gttcttgttt gtaggttgct aatttcagtt ctttttcttt taatgattct gcgatctctg
    12481 tttgtcgctg taattcagct gcttgtgatt ctttttctgc ttgagctttt ttgatacgcg
    12541 tttcttttag agcttccgct tctgctatat ctgcatctct tttgacttgg gcgattcttg
    12601 gtttccctaa agagtcaaga taaccgtttt tgtctctgac ctctttgata gtgaaagaaa
    12661 cgatgatcaa ccccattttt gcaaggtcta cagaagcgac ttcttgtacg ctttgactga
    12721 acttatcgcg attttgatag atttcttcta cagtcataga accaagaatc gagcgcaagt
    12781 gtccttctaa tacttctctt gcttcatttt ccagttcttc tcttgtcttg cctaaaaact
    12841 gttctgctgc tgtagcaatc tcttctacag atgaaccgat tttgatgatc gaagtaccat
    12901 cacacatcac cggtacgcct tgttcggtat acacttcagg ggtggagacg tccaatttac
    12961 tcgataatag actgatccga ttcgaacgtt gaaaaactgg caagacgaat gcgccacctc
    13021 cacgcacaat tttgattttg ttattgcttt catcaatgtg cacatttttg cttcctaaat
    13081 aacttccact gatgatcaac gcttcatccg gttttgctgt ctggtacttc gctacaaaga
    13141 cgatcagcaa catcagtaaa atcaaagctg cgattgcgat cgcgataaaa acaactggta
    13201 tttccattta acacacgctc ctttaagctt ttatcatgct ttcataaggc atgacataac
    13261 agattctttc cttgatctca atgatcaata ctttcgttcc ccgctcagct gtctgttctt
    13321 gggggctgta aaaacaagct ggacgactaa tggaaccggt aacacttttc aactgaattt
    13381 ctcccatacc tcgtacaggg atcggtgtga tcacaacagc tacttgtcct tccagagttt
    13441 tttcagaaga cgacatcgtt gattcggcat gtttcatcgg catcaatata tagaaattaa
    13501 gcaaaaatac caacactatc gatatgcctc cactagcaat aaataaaaat aaagatgatt
    13561 gatctgttaa acgctcacct agaaaaccaa ataagcttgt gaaagttaac caagggataa
    13621 taagcatcgg atcaagcggg ccatcaaaat cgaatacatc tccaaaaata atcaatagga
    13681 ctgccaatcc tgcacaaatc agcaatgtat accaataaat cgtctcaagt gcgattcccc
    13741 ctatcatata cacgcctcct tccatttcta cgtctatcat accaatgttt gaaaattctg
    13801 tatagcttct tttaccgact cagtggtgtt ttttatagga aggttgtgtt tatcgttgac
    13861 aatttgttaa atttttgttt tcatgttaaa attttcacaa taaattcttt cgaggtagcc
    13921 tatgtttcct ttttttaaga aaaaaccatt aaagctccag aacacgatca tccttgtcgt
    13981 ttgtttctcc acattcgttt ctttgattgt ttctgccata ttgatcagga attttattct
    14041 ttcgatgaac cgatctagtg tgtcagataa aatctctagc attgctaaaa tcgttgccaa
    14101 tgataaactc atccaaaaaa agctgcagac tggtgacccg acacatgcga ttcaaggaat
    14161 aacaaatgaa atcatgattg catcgaattt agattttatc gttgtcatgg accctgaagg
    14221 cattcgatat tctcacgcag acccaagtta catcggtctt cctttctcaa gtcatgaaga
    14281 tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa aaagaaggaa tgttagggtt
    14341 cggcttccgc tatttcgtcc ctatttatta tgatcaagag attattggtg cagtttgtgt
    14401 tggcttaaca aacaacacga ttgacaatca agtcaaagat ggacaaaaaa tgattgtttt
    14461 ttcattgatt attggactta gcgtaggcat cataggcgcc gtttttcttg cccaaaaaat
    14521 gaaaaaagta ctgcttggac ttgaaccaca agaaatagca gaacagttaa gtgaaaaaac
    14581 gattatcgaa aatgaagtag atgaaggaat cttagccatc tcttctgata aaactatcct
    14641 cttgatcaat acacctgcaa aaaacatttt ctaccaaaca gagcagctat tggatttcga
    14701 agtaggaaaa aaatcagtga taatctatat gacgttttgt ttaaagagac gattgagcat
    14761 caaacgaccg ttcggactca atccatgtat ttaaatggga tagaaactat ttctagcgtt
    14821 acacctcttt ttagcaatga ccgtttttta ggagcagtcg caactttacg aaatcgatca
    14881 gaagtaatga aattaatgga ggagcttggg gggacgaaac aatatataaa ctccatgaga
    14941 gctcaaaccc atgaattcat gaataaactt catgtcattt ctggtttgat tgatttgaag
    15001 aagtatgaag aagtcaccca ttttatccag cagctaaatc aaaattataa aaaagagatt
    15061 ggccaagtct caagtctgat caaagtacct gcaattgccg gttttttatt aggcaaaata
    15121 aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt tatccctaaa
    15181 atggagatgg atgactccat ccattcactt ttgcaagtgt tagggaactt acttgataat
    15241 gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac aggtccggat tcatttaaac
    15301 tatgacacag aagctgaagt attcatcgtg accgtctgcg atggcggtgt cgggatatca
    15361 gaagaaatct cagaaaaaat atttacgcta ggtttttcga cgaagggaat caatcgtggg
    15421 tttggattga atctcatcca ttcaatcgtc aaacgccacg gtggattttt agaattttcc
    15481 aaacaaacaa atggcggtac aatcgtctat ctagaattac caaaaaagga agatcaaaat
    15541 gatgacaaat gtcctgataa tcgaagatga tccaatggtt gcagaattga atcgtacttt
    15601 tgtggaaagc atccctaata tgaacgtaat cgaaagtttc agaaatgtag aagaaggaaa
    15661 aaaatatttc agacaaaaaa acgcagatct gctgctgctt gatgtataca tgcctggcga
    15721 aagtggtatc cattttttat cttggcttag acagcaagga aatgatatcc cagtgatttt
    15781 gatcactgct gcagatgata tcgaaaccat aaagaatgtg atccgctacg gtgccttaga
    15841 ttatttgatc aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa agtatcttgc
    15901 tttctctcag acaatccaaa cgactgaaaa agccgatcag aaattgatcg atcaagtatt
    15961 caaccacacc actcccattc acgaaatacc atcattcatc gatttgccta aaggtctgtc
    16021 aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta ttttttcaac
    16081 aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat atttgaattt
    16141 tttagtagaa caagatatat tgaacgaaag aatcaaatat ttggaaatcg gccagccgct
    16201 aacctgttat gaattgacaa agaatgggcg gaaaaagctc acagattact gttgagtcat
    16261 cgaattatcc catacaaaat agtccaagta tacagaagta acaactgttt acttggacta
    16321 tttttaaaca ttttattgac gaatatccat gttttcttat cttaattgac gggctttagg
    16381 aagagatacc tcatttttcg tttttccagt ttctattatt tcacgaaagt tctcgaaact
    16441 tgtcgaaatc atatttgata atgcctcatc tgtatttgaa cctacatgcg gggttacaag
    16501 aactctaggg tacatttcta ctaattgctg gatagcaggg tctggaatcg tctcccatgg
    16561 cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg caaaaccagc
    16621 taaatgattg cttttcaaag ctcttaaaat agcttcatta tcttgaagct ctccacgggc
    16681 tgtattaatc aagatgctac cttttttcat ttgagctaaa aatccttcat tgatcatttg
    16741 atcgttttca cctgggatat gaggtacatg aatactgaca atatcgcttt tggctaataa
    16801 ctcttcaagc gagtggaacg tcaatacttt tttagcagct tctgtctgat gaatatcgta
    16861 gccgatcact ttggctccta gtcccttaaa caaaacagct tccgtcaacc ctatcctgcc
    16921 tgttcctatg atccctactg tgcaatttct gatttcacga ctaaacattt gctcatccac
    16981 tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca gtatttctca aaagcatcat
    17041 ggcaaaagtc aaagataact ctgctatcgc atttggcgaa tacgatggta cacgggctac
    17101 tgtcatacca aaatcagctg ccgcttgtag atcgatatga ttgactccca cagtccgtgt
    17161 aaaaacataa cgaatcccat attcctgcat ttttgcaaga ttggtctcat ctgctataca
    17221 atttcccctc aacaatacag catcatggtc ctttgctgac acgatattgt catgtgtcaa
    17281 cagttcttca taaagactga gatcataatg gtaaatattc aatttttcaa aaaaatctat
    17341 ctcgttcgga cgaacaccgt aacatgcgat tctcatactt tcttcctcca tttcttatga
    17401 aaataaacct atgccatgaa tcatttgtaa tacaacgatc catacaggtg tcagtaccac
    17461 agcaaaaata gttgacagta aggatgcatt ggaggctaaa agtgcctccc tatcaaaact
    17521 gatagcatag gctgctgcca ctgttgcagc tggtgtagcc atcatgataa cgattgttgc
    17581 cagtgcttca aaattgactg gtagaatatt cgttgccgtc aaaactgcta gaatcacgat
    17641 attcaatgca ggtacaaata ccactttgac tagactgtaa taccatgaag ttttttcaga
    17701 tgcagctttt ttgaagctta cttcacctaa tgttgcgccg attgctagcc acgccaatgg
    17761 tgaacttaaa ccagctaaat aatccatcgg ctgaaatagc cacggtgcag tctggtcaat
    17821 tcgcaaaaac gctacttggc ttatctctcc ttctgcacct gtaactgaca cttgtggcag
    17881 atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga ttggattgac
    17941 gaacatcgct ttgatatttt tagcttccat cttcaagcca ctcattttta tgtatccata
    18001 agaatacaaa aagattcggt agccaatatt gaaaattgag gaatacatca cgccgacagg
    18061 accataaatc gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa agatcgtcag
    18121 gacacgcaac gttgtttgct tatccccttt atagcttaag aaaaaaggtt ttgaaactag
    18181 aataagaata atataaataa ctaatcccca aatcaagaca ttgattcctt gcatcagtaa
    18241 ttctgtattg atatcttgca tgaaagctcg aaatgccagt gctggcaaag ccactgttaa
    18301 gacgactttc gatagaattt ttccaacaga ttcagaaaaa atcccctttt ttctacaaac
    18361 aaaccctaat aatataataa aaactgttga agtgattgca ctgacaatcg acata
//
LOCUS       DACDID010000047        18018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDID010000047 DACDID010000000
VERSION     DACDID010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.047"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IV236_001251"
     CDS             complement(<1..456)
                     /locus_tag="IV236_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8771459.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IV236_001252"
     CDS             complement(561..1424)
                     /locus_tag="IV236_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8771460.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IV236_001253"
     CDS             complement(1620..3719)
                     /locus_tag="IV236_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8771461.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IV236_001254"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IV236_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8771462.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IV236_001255"
     CDS             complement(5072..6913)
                     /locus_tag="IV236_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8771463.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IV236_001256"
     CDS             complement(7095..9488)
                     /locus_tag="IV236_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8771464.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IV236_001257"
     CDS             complement(9595..10455)
                     /locus_tag="IV236_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8771465.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IV236_001258"
     CDS             complement(10514..11035)
                     /locus_tag="IV236_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8771466.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IV236_001259"
     CDS             11355..12074
                     /locus_tag="IV236_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8771467.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IV236_001260"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IV236_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8771468.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IV236_001261"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IV236_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8771469.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IV236_001262"
     CDS             16434..16790
                     /locus_tag="IV236_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8771470.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..>18018)
                     /locus_tag="IV236_001263"
     CDS             complement(17031..>18018)
                     /locus_tag="IV236_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8771471.1"
                     /translation="KAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQKISSAQT
                     KTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVLYELDTV
                     MYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLTDWTYEL
                     IEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFDIMADLK
                     EAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEVQEISEK
                     KFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL"
BASE COUNT         4930 a         3742 c         3133 g         6213 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttt
//
LOCUS       DACDID010000048        17728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDID010000048 DACDID010000000
VERSION     DACDID010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.048"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..1806
                     /locus_tag="IV236_001264"
     CDS             121..1806
                     /locus_tag="IV236_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8771472.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1820..3103
                     /locus_tag="IV236_001265"
     CDS             1820..3103
                     /locus_tag="IV236_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8771473.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3173..4042
                     /locus_tag="IV236_001266"
     CDS             3173..4042
                     /locus_tag="IV236_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771474.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4039..4875
                     /locus_tag="IV236_001267"
     CDS             4039..4875
                     /locus_tag="IV236_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771475.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4901..5968
                     /locus_tag="IV236_001268"
     CDS             4901..5968
                     /locus_tag="IV236_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8771476.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5965..6792
                     /locus_tag="IV236_001269"
     CDS             5965..6792
                     /locus_tag="IV236_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8771477.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6837..7862
                     /locus_tag="IV236_001270"
     CDS             6837..7862
                     /locus_tag="IV236_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8771478.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7859..10042
                     /locus_tag="IV236_001271"
     CDS             7859..10042
                     /locus_tag="IV236_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8771479.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10042..11172
                     /locus_tag="IV236_001272"
     CDS             10042..11172
                     /locus_tag="IV236_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8771480.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11169..11846
                     /gene="pgmB"
                     /locus_tag="IV236_001273"
     CDS             11169..11846
                     /gene="pgmB"
                     /locus_tag="IV236_001273"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8771481.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11930..12457
                     /gene="msrA"
                     /locus_tag="IV236_001274"
     CDS             11930..12457
                     /gene="msrA"
                     /locus_tag="IV236_001274"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8771482.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12658..13344)
                     /locus_tag="IV236_001275"
     CDS             complement(12658..13344)
                     /locus_tag="IV236_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8771483.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13424..14962
                     /locus_tag="IV236_001276"
     CDS             13424..14962
                     /locus_tag="IV236_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8771484.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15082..15549
                     /locus_tag="IV236_001277"
     CDS             15082..15549
                     /locus_tag="IV236_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8771485.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16068..16511
                     /gene="rplM"
                     /locus_tag="IV236_001278"
     CDS             16068..16511
                     /gene="rplM"
                     /locus_tag="IV236_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8771486.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16527..16919
                     /gene="rpsI"
                     /locus_tag="IV236_001279"
     CDS             16527..16919
                     /gene="rpsI"
                     /locus_tag="IV236_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8771487.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17007..>17728)
                     /locus_tag="IV236_001280"
     CDS             complement(17007..>17728)
                     /locus_tag="IV236_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8771488.1"
                     /translation="TTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDF
                     NNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKG
                     RKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADY
                     LNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVN
                     TPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         5762 a         3248 c         3805 g         4913 t
ORIGIN      
        1 ctttcttatt tgtatttttg tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa
       61 gcgttttcca taatatgatc aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg
      121 atgtatacaa aacttttaca agcgatttat cgcgactcag atttgtctga gaaaaccgcc
      181 tctatgattg aacaaaaagt cagtgtttat cagcagaaga aaggccaggc aggcaaactg
      241 cattgggacg aagaagatat tttcttgatt acttatgggg atcaattttt tgaagagcag
      301 caaccaactt tgcgtacatt taagaaattt tatcaaaagt atttggcaga tacatttaga
      361 gttgtacatt tcttgccatt tttcccatat acatctgatg atggtttctc cgttgtaaat
      421 tatcaggcaa tcaacgaccg tttgggcaca tgggaagata tagaagaaat gagaagcgaa
      481 acacggttaa tgttcgactt tgtctgcaat catatgtctg ccgaaagtac ttggtttcag
      541 gaatatttaa acttgaatga tacgtacaaa gattttttta ttgatttgcc actggataca
      601 gatacaagca gcgtgactag accaaggacc agcccgttac ttacaacgtt cattgacaaa
      661 aacgggaaag aaaaacacgt atggacgact tttagcgaag atcaagtaga cttgaatttt
      721 aaaaatccag aagttttatt gaaaatgatc gatattcttt tgttttactt agaaaaaggc
      781 gcttcattta ttcgcttgga cgctgtggga tttttatgga aagaactagg aacttcttgt
      841 atacacctgc cagaaacgca taaaatcatc caattattcc gtgcaatcgc tgatgaagca
      901 gctccaggaa cggtattgat cactgagaca aatgtgccac acaaagataa tatcagttat
      961 tttgggaatg gcgagaatga agcacagatg gtctatcagt tttcgctacc acctttgacc
     1021 cttcaagcga ttcgttcggg taatacaagt aagttgaaag aatggttaaa aaccatcgat
     1081 ttcgcatcga aaaaaacaac tttttttaat tttttagcct cccatgacgg tatcggattg
     1141 aatccggtac gaggtattct gccggaaaaa acgatccttg aattagtgga acagctgaag
     1201 aaagagggtg cccgagtaaa ctacaagaaa aacccagatg gaacgaccag tccatatgag
     1261 ataaacgcta cgtatctaga tgcgttaagt tctccaacag atacgacgga atggagaagc
     1321 aaacgctttt tagctgctca ttcatttttg gtaacgttac caggcgtgcc ggcaatttat
     1381 attcaaagtg tgctgggaag tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat
     1441 cgaaaaatca atcggcaaaa atttttgtta gcagatattg agaaagagtt gcagacagat
     1501 ccattaagaa cagcagtata tggtgaattg acccagatgc tatctattcg aaaaaaagaa
     1561 aaagccttcc atccgaatag tttgatttac ttactggact cagcagattc ttttatcagt
     1621 tttattcgcg aagcgaagga agagaaagtc ttagttatcc ataatttttc caatcaatca
     1681 gcaggttatc ttttaccaaa aggagattac ctagacctgt tttctggaca aattatccat
     1741 tgcagtcaag aacgaaagtt gatattccag ccgtatgaat atatgtggct gaaaataata
     1801 aaataagagg tggaaaaaaa tgaaaaaaat tattgttact acctgtctgc tagctactgg
     1861 atttcttctt actgcttgtg gtgaaagtca aagctctaac gaatcacaac agacaacgat
     1921 tcaattcatg cactcttctg tcgaacagga acgattaagt gtgatcaatg aactggttgc
     1981 tgattttgaa aaagaaaatc cagacatcaa aatcaagcaa gtgccggtag aagaatcagc
     2041 gttcaataca aaaatcgtta cgttagctcg ttccggtaaa ttaccagaag taatggaagt
     2101 cagtcaggat tttgctaaag tgatggataa agatgaactg attgatcaga cggcagtcca
     2161 aaacgttatc gaggaagctg gagaagataa ttattatgac ggagccaaaa accttgttcg
     2221 ttcagaagat ggaaaaagct atattgctgc accaattagc ggctgggttc aaggtatttg
     2281 gtatgacaaa gaaaaattat cagaagctgg tttttcagaa ccagaaaatt gggatgatat
     2341 tttaaaaatt gctcaacatt tcacagataa agagaataaa caatatggaa ttgctatgcc
     2401 tactgctgaa ggaacaatga gtgagcaagc gttctctcaa tttgctttgt caaatggagc
     2461 aaatgttttg gatgatgaag gcgaggtaac gatcgatacg gaaaagatga gagaagcatt
     2521 gtcgttttat caaaacttat cccaatacac gatgccaggc tctaatgatg tgacagaaat
     2581 caaagatgct ttcatgaatg gaacagcacc aatggcaatc tattcaactt acattttgcc
     2641 ttctgtttat gaagcgggga actcagaaaa tatcggtttt gctatcccaa cacaaaaaga
     2701 tcaagctgtc tatggcacag tatctggatt gacgatttct tctggtctag acgagaaaca
     2761 aaaagaggct gctgaagcat tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg
     2821 ggtcttgatg tctcctggtg gtgctcaacc agtgaataaa caagtagtcg aactggatgg
     2881 ttacaaagaa aatgaagtca tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc
     2941 ttttgatgaa gtacaagtct ttggactagt cggggaaaag aatttcacga aaatgggaga
     3001 tatcacaagc tcaggtgtga tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga
     3061 tgtcgatcaa gctttagctg acgcacaaaa aaatacgaaa taacaagaag ttctgccaat
     3121 ggtgttcgaa aaaagtagat agctattggc agattatcag ggggaggttt caatgaatcg
     3181 tccaaggaaa cattctgact taaaattagc catgggactt ttagcaccaa gcatgctttt
     3241 attagctgtt ttagtgctga ttccaatgct ttccaatatt cagatcagtt ttttcgatat
     3301 gccgctgaat cctaactcag acgccgtttt tatcggatta agcaattatc ttgaaatttt
     3361 aagtgatagt gaattttatc gttctttagg tttaacagtt gcttacacat tcttagttgt
     3421 agcaggcagt acagctttag gattagccgt agctattttg cttaaccgca agttcagatt
     3481 taggaaaacg gtgcgttcac tgattttatt aagttatgtt acgccatcta tttctttgat
     3541 ttttgcttgg aaatatatgt tcaataatag ttatggaatc gtcaatgatc tgttagtaga
     3601 taggttgcag cttttcaacg aagcaccgct gtggttcgac cagccagttg ctagcttctt
     3661 tatggtcgtt ttgtttgcta tctggagata ctatccgtat gcgtttattt catttttggc
     3721 tattttgcaa acagtcgaca aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc
     3781 ttggcagaag ttcaagatcg tgacactgcc ggcaatcaaa ccagtactag caactgtgat
     3841 tgttttacga tctatctgga tgttctatat gtttacagat gtctatctac tgacgaataa
     3901 agtcaatatc ttaggcgttt atttgtatca gacagccttt gcgttcaatg atcttggaaa
     3961 agcagcagct atatcgattc tattgttcat cattattgca gcggtcgtct tgctctcacg
     4021 aaagaaggtg gacttgaaat gaaaaataaa aagagcagga aaatcttatt ttacgcgacg
     4081 attttgttgc tattagctgt caatttgttt ccatttttga tcatgctgat gacatcattc
     4141 aaggattcac aagaagcaat tgcaacgaca caaactattt ttccgaaaga atggactttt
     4201 gaacattatc aggatatttt caatgctgag atttttccat tcatcatgta tttcaaaaat
     4261 agtttagtga tctccttgat agcctccgta actgcagtag tgattgggat cagcggggca
     4321 tacgctttgt caaaactacg cttcaaagct cgctcgaaaa tcaatgctag cttttacacc
     4381 gtttatatgt tttccggtat tttgatcgtt gttccattat ttaaaatcat ttccggtcta
     4441 ggtttgtatg ataccaatac agcactgatt atcacgatgg tcgttcaaac acttcctact
     4501 gcgatattca tgctaaaaag ttattttgac acgattccga ctgatattga agaagcagca
     4561 atgatcgatg gtcttagccg gatccagatc attattcgga tcattatacc attggctatt
     4621 tctggaatca tttctgtctt cgtttattgc tttatggttt cgtggaatga ttatttattt
     4681 gcttcgattt tcctgtcgac ctccgaaaaa tttactttac caatcggttt gaacacatta
     4741 ttcagtacac cagactacat ttggggacga atgatggccg cttcattggt gaccgcttta
     4801 ccagtcatca ttatgtatgc gttatctgaa cgatttatta aaggtgattt agtcgaaggc
     4861 ggcgtaaaag gataaaaaga aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt
     4921 agaaccaaga atagccggat tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt
     4981 acgtatccga gtaattttcg ctactccaaa accaggaaca gaaattgtcg actttagagg
     5041 attaagtcca tttatcgatg aagaattttc agaagaatgg cgcctattct tgccaagaga
     5101 agaaggaagc caacgcggtg tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg
     5161 tacgatcgaa gaaacgggca gtttggtgac agagtatcaa aaaggagagt tagtctgcac
     5221 ttatggaccc attcgtgaaa cgctgattac aaacggtgtt gataattata aactacgtaa
     5281 acttgatcgg ttggatcaat ggaaaaatgc agtatgttac gatcctgctc aattcgcttt
     5341 aggtggtatc cgggaagcac atgttcgagc aggtgatgct gtagcaatct tcggtttagg
     5401 cgctatcggg caaattgcga ttcaattagc taaaaaagca ggtgctacta ccatcgttgg
     5461 gatcgatccc atagcgcagc gtagagaatt agcagaaaaa cacggtgccg attacacgct
     5521 agatcctatg aaagcagatg tcggttttcg tctaaaggaa ttgacagata aacgtggagt
     5581 agattcagtt attgaaacga gtggcagtgc tgccgcatta caagcttctt taatagggat
     5641 cggctatggc gggatcattt cttatgttgc ctttgctaaa ccttttccag ctgggtttaa
     5701 tcttggtcgc gaagcacatt tcaataatgc aaaaatcgtc tttgctcgag catctagtga
     5761 accaaaccca gattacccaa gatggaaccg taaacgaatc gaggacacat gttgggaatt
     5821 attgatgaac ggttacttgg attgtgaaga aatcattgat ccagttgtgc catttgcaga
     5881 aagcgcagaa gcttacatgc gttatgtaga ccagcaccca gaagaaagta tcaaaatggg
     5941 cgttcaagtc cgaggaggag aacaatgaaa ttcggtacgc aagatcaagc atttttccca
     6001 agagacattt tagaaaaatt tagatttatc aaagactgtg gatttgaaga atacgaaatc
     6061 gacggtaagt tgttaataga aaatatcgac acagtcaaac aggcaatcaa agaaacaggg
     6121 atcccagtag tgacagcttg taacggctac gatgggtgga tcggtgactt tatcgaagaa
     6181 cggcgtctta atggtgtacg tcaaattgct gagattttgc gtgcactaag cgaggttgga
     6241 ggcaaaggaa tcgttatccc agcagcttgg ggaatgttca cttatcgatt accgccgatg
     6301 gtttcacccc gaagcaaaga aggagactat aaagcagtca gcgaatcact tcgttattta
     6361 gataaagtag ccgaagaaac aggtacggtt atttatttgg agcctttgaa ccgataccaa
     6421 gaccatatga tcaatttgtt agcggatgcg cgtcattaca ttgactctaa tcaattgaaa
     6481 catgtgcaga tcatcgctga tttttatcat atgaatattg aagaagatga tatttcaaaa
     6541 gcattgacta cgcaaaaaga gtatgtgggt catatccatt tagcagataa tcatcgctac
     6601 caaccaggat ctggcgcaat tgattttact cgccattttc aaacgctact tcaaaatgag
     6661 tatgaaggtt atatggtata tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat
     6721 caggaatcat tgcaatactt gaaaaactgt ctggaaacag cgcgtcattt aaatgattta
     6781 gagaaagaat agagaaagaa taacaataag taaataagca aaggagagag aaataaatga
     6841 agaaagtagc aatcataggc gctggacaag tagcagaaaa agtacatgct gcttattatc
     6901 aaaaaagatc agatgtctta gaattggccg ctgtggttga tccagtcagc cagcgagcag
     6961 aagacttttg ccgacgtaca ggattttctc atgcgtatac atcagttaca caaatgctga
     7021 atgagatttc tcccgatatc gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg
     7081 tattggctgc cctcgaagct aatgcttcag tgatgtgcga gaaaccacca gctatgacag
     7141 ctgatcaagc aagaaagatg gcagagacag ccgataaaaa cgggcagatt ttggcttatg
     7201 gattccagca tcgtttttct gacgaagcaa caatgatcaa gaatcatctt gatgttctcg
     7261 gggatattta ttacgtagaa gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa
     7321 cgtttatcga caaagaactt caaggaggcg ggcctttgat cgattatggc attcacatgc
     7381 ttgatacagc gatgtatatt actggatttc ctaaagtaaa aagcgttcgg gcgtacagct
     7441 tccaaaaaat tggtacgaaa aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat
     7501 tcacagtaga agatgcaatt tttggcacac tggaattaga aaacggcaag atcatccaac
     7561 tgaatacttc gtttgctttg aatatcagag aagacaaact gaatgtggct ttttgtggcg
     7621 atcaggcagg tgctaatctg tatccaggcg aactattcga agatgtagat ggaaagctgg
     7681 tttccttaca aaagaccacg ccagttaatg acccacatga actaagtatg gctcattttg
     7741 tgaatcgggc gctcggtgac cagactcaga tgattgcaga tggctggcaa ggatatgtca
     7801 tccagaatct ggtggaaaat ttatatcgtt cggcagaaac aggagaagtt attaccttat
     7861 gaaagtagaa gaaaaacagt ttgatttaga gtatatcaat aagtatgcga gtcagatgac
     7921 cattggaaat ggattttttg gtatcagagg aagccaggag gaagactatc ggaatcaagt
     7981 aaggggaatg tttgcagctg gtgtatataa ccgaccttta ggagcagaat cagccgagtt
     8041 agtgaatctc ccagatgtca tcagatggga aataaaaatc gacggtgaac atttttctct
     8101 tgagacgatg aaagttcttg catacgagcg ggcactagat ttagaaatgg gagagcttaa
     8161 acgctatgcg ctcattgaaa ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt
     8221 tgctgatcaa aataatctcc atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga
     8281 agactgccaa gtagatgtga aaacgggtat cgatggacag caaaccaatt ttggtacaca
     8341 gcagttggtt gaaaaagaac tgcgagtatt tggtgacgat ttgatcagtg catcctacca
     8401 gacaacagaa agtaaaattg atattgggat tgcagctaaa ttcaataaag caggtgtatt
     8461 ccaagcaaaa aatcgccgga tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc
     8521 ctttacgctg gagaagatca tcgtgctctc tacctctctt cagcaaacag acccgtttaa
     8581 gaaaagtatt gcccaagccc agaaaagcgt tagttataca gaaattttag agcagtcgat
     8641 gtcttattgg acagattttt ggcatcagca tcggataacg atccaaggcg attcttcttt
     8701 cgatcagtta gcgatggact tcgctgctta ccatttacag attatgactc cccgcaatga
     8761 tccaagtgtt agtgtagggg cgaagggact aactggcgaa ggttataaag ggcatgtttt
     8821 ctgggatacg gaaattttta tcctgccttt ctttttatat cagtttcctg aagtagcaaa
     8881 gaatttgctg ctgtaccgtt atgatcgatt agaaggcgca cggcaaaaag caagagatta
     8941 tcactatcaa ggtgcacttt tcccatggga aagcgcacac actggtcagg aagagacacc
     9001 caaatttgct gcattgaata tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc
     9061 agaacatcat atcagtgcag accttgctta tgccgttcaa gagtatgtcc aagcaacaga
     9121 ggatcaatcg tttttagaaa caaagggaaa aacgctcatt catgaaacag cagctttttg
     9181 gctgagcaga gctgtaaaag tcgatgatca tttagagata cacagagtca ttggtccaga
     9241 tgaatacaca gaatatatcg acaataatgc atatacgaac tatctggcgc attataatgt
     9301 ggccttagct gttaaatatc aagctggcga tccgcttttt ttgaaagaat gtcaaaattt
     9361 tttagataag ttatatttgc cagtgcctaa caaagaacag ctgattccgc aagatgacac
     9421 gtatctcagt aagccagtta ttgatttgac cacttacaaa gaacgtcctg gcagccagtc
     9481 tattttactt gattatgccc gtagcgaagt aaatgaactc caagtcagca agcaagcaga
     9541 tcttgtcatg ctgttatact tattacctga gtatttttct caaccaatcg tttctaagaa
     9601 tttgcattac tatgaaaaca aaacaatcca cgactcatcg ttgagtaaag cgattcacgc
     9661 catagaggct tgccgtatcc aagaaatgga atgggcgtat catttattcc aagaagcgtg
     9721 cctgattgat ttaggaccag atccgcattc atcagatgac ggaattcatg ctgcttcatt
     9781 aggagcacta tggctagcag ttatatttgg tttttcaggt attacagtaa cgaatggaac
     9841 tctttctatt tctccaagac ttccttcagc atgggaaaaa atttcttttc cttttatttg
     9901 gaaaaatcac aggttgctat ttgttttgac aaaaaaacaa atagtaataa aaaaagagac
     9961 gaaagaaaca ttacctattt ttgtcaatgg agaccaatac cagctggaaa aagaactgat
    10021 cttggaacgg aggggaaaat aatgcgtatt ctgattgcgc cagattcttt caaagaaagt
    10081 ctgacagcct tagaggcagc acaagccatt caatcaggtt ttcaagaagt ttttgttcag
    10141 gctgaatatg atctgctacc tataggcgat ggaggagaag gaacggtttc atcattagca
    10201 gacagtttaa ggttgaagcg gcaaacagca gtcgttacta atgcatttgg caaaaacaaa
    10261 gaaatccagt acgcttcaga cggtcaaact gcgatttttg aaatggcaga aatcgtcgga
    10321 ttgaaagatg tcccactaaa ccaacaacag ccacttaggt tatccacgcg aggagtagga
    10381 gaactgatac gacaattgtt gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga
    10441 agtagtacca acgatggcgg catcggcatg gcagaaggtc taggttatgc tttttacgat
    10501 caagataatc aaaaagtaga agcactggga gaaaatctag gtaaaattgc tacagttgat
    10561 aaaacgaacg tgcctattga gctgattaaa gccaaaatca ctgtcattac tgatgttact
    10621 aatcttttat gcggaaaaga aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa
    10681 gaaaatgaat tagcagtggc tgaccaagcc atgcatgatt tttatatgag atttgcccct
    10741 caagtaatcg aggcatcggg cagtagtgct ggtggtggaa tgggtgcagg actgttagca
    10801 tttgcttcag ggaaaatagt ctctggcatc gactacgtat tggatttact ggagttcgat
    10861 aaacgggtac agcaagcaga cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt
    10921 ctaaaaggaa aagcgccagt tgggattgct agaagagtac cagagggcgt tcctgtgata
    10981 gctatttgcg gaagtgttgg taaaggaagc gaaaaagcag cagatgttgg catttcggcc
    11041 gtttttccga tccttcctgc agctgtgcct ttacaagaag ctttacagca agccactgat
    11101 aatcttcatc gcacagctaa aaacatcgca tctttacttc aagtcgttca ttttcaagga
    11161 ggtaatcgat gaaaatcaaa gcagcactat tcgatttaga tggcgttctt gtagacacag
    11221 ctcgttatca ttacgaagcg tggctggttt tagctaacca gctgtccatt ccattcactg
    11281 agaaagaaaa cgagcaactg aaaggaatat ccagaaccga gtcactagag cgattgctgt
    11341 catttggaaa aatggaacag aaattctcag aaaaagagaa atcagccttt gctgagcaaa
    11401 aaaacaatct atatcttcaa gcgattcaaa aaatggatga aacatccgtt ttgcctggtg
    11461 caatagctgt tcttgagtac ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa
    11521 gcaaaaatgc acgcctcatt ttagaaaaaa cgaatttgac aagttatttt gatgtcctta
    11581 ttgacggcac gcaagtatca aaagcaaaac ctgatccaga agtttttctc aaaggagcac
    11641 agcaattgaa tgtaccgcca aatgcatgct tggtcattga agattcagaa gctggttgtc
    11701 aagcagcttt ggctggtaac atgcatgtat tgggaatagg tgaaaacata aatcttcctt
    11761 ctgctgaata tgtgattcct gatttaaccg tttttgatca agtacgttcg ttttggcatt
    11821 tatcagaagc cgtatcacgt atataatagt ttccgttttt tctgtcacaa gcaaaacgtt
    11881 atcctccttt tggaaatcaa ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa
    11941 agcagttttc gcaggcggat gcttttggtg tatggtcaaa ccatttgaag agcagccagg
    12001 gatcatttct gtgacatccg gttatactgg cgggcatgta ccaaatccaa cttatgaaca
    12061 agtaacaaca ggcacaacag ggcatacaga agcagtagag atcgaatatg atccagaagt
    12121 tatttcttat gatcagttgg tggaaatcta ttggcagcaa accgatccga ctgacgcatt
    12181 aggacagttc gctgatcgcg gtgattctta tcgtccagtg atctactata atgatgaaga
    12241 agaacgaaat attgcagagc gttcaaaaca agcattgcaa gacagcgggc gattcgataa
    12301 gccaatcgtg acaaaaatcg aaccaagaag cgaattttat cctgcagaag attaccatca
    12361 ggattattac aagaaaaaca agctccatta caatctttat cgagaaggtt ctggacgtgc
    12421 aggtttcctt cgtcgacatt ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg
    12481 aacaccgtat atcagaggtt gtaaaaccag cattttgagc taatactgag ggtcagctga
    12541 ttgaacacca tttagtggag aggttgtgaa cctgtcgcct agctccaagt cacaaggatt
    12601 tggtaagaga acactatata tcagaggttg tgacaagtcg tttgtcacaa ccttttttca
    12661 atacctaaga tgaaggacaa tcagcaacgc actgctcaaa aaaggaatga aaggcagctt
    12721 ttccaacttt tttttatgac agataaagta aacagaaaaa acggatattc caaacagact
    12781 agcaagaaac aagatctgaa gcaactcttc tagagacaag ccaccactcc aagaaagcat
    12841 caacagagta tcgccagctc cgagatagga ttgaccatag cgaaggcaca caccgcctac
    12901 gataaacgtt aacagcacac tttgccattg gaagacgccg gcctgcaacc aaaaaatcca
    12961 taaaattgcc caagaacaga atagcgtgtg agaatggacc atgaagtaaa ataaatccat
    13021 caacgaaaat agcaaagccg aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga
    13081 aaaagtaaag aaccaggcaa aaagaccacc acataaggct tctgccaaca aatagtagaa
    13141 gggcaaccga cacctgcaat agcggcagcg aaatcgttgg acgatgatcg agagaatcgg
    13201 aatcaattca taccaagaaa gcgaacgaca gcatttgaca caatgagatc taggataaat
    13261 aatagagtga cctaacggaa tccgctgggc ggttaaacat aaaaatgatc cgatacagca
    13321 accaatgata aaataaagta acacgccatc tccccctttt caaaaccaag atacgcaagc
    13381 ttaaactaaa tttttgtaca tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg
    13441 attagatatc ggaaccacac aaaccaaagc agttgctttc acagatgaag cccaagaagc
    13501 agcatccgct tattttcgct accctttgat ccaagaaaca caagggatgg cagaacaaga
    13561 tcaggaattg atctttcaag gtgtctgcac cgttataaaa gaagtaatta aacagttgcc
    13621 ttcagacaca atcattcaat ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt
    13681 ggatgaagag aaaacaccgt tatctcgaat gatcacttgg gcagataacc gagcagcgga
    13741 cgaagcagaa aaactgaaat ataccgtgct tggcaaagaa ttttatcggc agactgggac
    13801 gccgattcat ccaatgacac cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga
    13861 attattcaaa aaaactgctt attttgtcgg tatcaaagac tatatctttt atagattgtt
    13921 tggcgaactg atcagcgact acggaacagc ttcaggaact ggatattttg atatccatga
    13981 attccaatgg gcagaagaag tgcttgacta cttgaacatt cgaatagagc aattgccgca
    14041 agtctttcct tccacctatc aagtaacagg tctaccatca aaaacagcag cagaactagg
    14101 tttgccaaat gatatatcat ttgttttggg aggggcagac gggccgcttt ctaatctagg
    14161 gttaggtgct tttggtgata cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg
    14221 ttttattact actcagcctc agttccatcc agaaatggag acgttttgtt atgttctgga
    14281 tcagaaacat tgggtagtgg gaggagcgac tagcaatggt gctggcatct ttgattgggc
    14341 agggcataca tttttacagg aagtcgttca atctgctaaa gaaaaaggag aaaatccata
    14401 tgatgcactg ctttcggcta tcgcttttgt gccagcgggt gccaatggtc tgatttttca
    14461 tccctattta cttggtgagc gagcaccgct ttgggacgca gatgcgtttg gcagttttat
    14521 cggtttacag cggcaacata cagaaaaaga aatgatgaag gcgattctag aagggatctg
    14581 tctcaacctt tatcggattt taaaaggagt ttgccaggaa aatcagctca cagagatccg
    14641 tgctacaggc gggtttgctc aatctccgat ttttcgtcag atgatggctg atgtactggg
    14701 ttacccgctt gttttcccta ctgtcacaga agcatccgca ttaggagctg tgattttagg
    14761 ctggagaagc atcggaaaga tccaaagtat cagtgaagca caggaattga tagaattgaa
    14821 agagcaagtt ggagtacaag aaactgatca taaaaaatat cagaaaatct atccattatt
    14881 tgtttcgaca caaaagcatt tagcacagac ttatcagcaa tttgctcaac tgcgagaaga
    14941 attagctgat tctgaaaaat gattggcttt acggataaaa aagcgaactg aaaatcgttc
    15001 tcaggtaaat gattttgttg tacttttcat tgaaagtcta ctattatttt aagtgtaaga
    15061 aaatgttagg aggcaagaaa gatgaaattc caacaaacca aagaagtatt aaatcaatta
    15121 gttgcagatc taagccaaat gtctgttgtt gttcaccaaa cacattggta catgagagga
    15181 ccaggtttcc ttacattgca cccaatgatg gatgagttca tggatgattt gaacgctcag
    15241 ttagatgaag tatccgaacg cttgatcaca ttagacggtt caccttattc tacattgcgt
    15301 gaatttgcag ataacacaaa aatccctgat gaaattggcc gctgggatcg tactattcca
    15361 gaacgtttgg aaacacttgt tgcaggctat cgttatctag cagatcttta ccaaaaagga
    15421 atcgaagttt ctggagaaga aggcgacgat ccaacacaag atatctttat cgaattcaaa
    15481 acagccacag aaaaacgtat ctggatgatc caagctcatt tgggcaaagc gccagagatc
    15541 gacgcgtaaa aactgttgtt gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga
    15601 cctgagtatt agagaaacaa acgtcaaaat agttcctcat attttgccgt tttttgagct
    15661 aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag
    15721 ctccgagtca caagaaacaa ttttaaaaaa cagcttctta tgttttatgg atattgtgac
    15781 ttgtgaccga ggagttggca ggagaacacc gtttattcaa aataaggggt tgtgacaaga
    15841 cttttgtcac agcctctttt tgtttttcat tttgacagga aacgaccctt ttatataaaa
    15901 gaaagaaaag aagtaaagtt tcatataaat ttttcaatca agcagaaaaa cgttgacatt
    15961 gactagatta gcgggtaata tgttaaatgg tattgtttgc cccacaaaga gccccggaaa
    16021 ctcgaagtgt atgtcaaaca tcagaactta aattggagga aaataacgtg cgtacaacat
    16081 atatggccaa accaggcgaa gtagaacgta aatggtatgt agtagacgca actgatgttc
    16141 cattgggacg tctttcaaca gttgttgcat ctgtactacg tggtaaaaat aaaccaactt
    16201 tcacacctca tttggatact ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat
    16261 taacaggtaa aaaagcgact gacaaaattt attaccgtca cagcaactac ccaggaggat
    16321 tgaaatcaat cactgctggc gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt
    16381 ctgtaaaagg tatgcttcct aaaaacactt taggccgcaa acaatttaca aaattgaacg
    16441 tatacggtgg agcagaacat ccacatgcag cacaacaacc agaagtttta gacatcacaa
    16501 acttaatcta ataggaggga atttcattgg cacaagttca atatatcggc acaggccgtc
    16561 gtaaaaacgc agttgctcgc gtacgtctag taccaggaac tggcaaaatt actgtaaata
    16621 aaaaagatgt tgaagaatac attccacatg ctgacttgcg tgaagtaatc aatcaacctt
    16681 tcgctgttac tgaaacaaaa ggcgcatatg acgtatttgt taacgtaaac ggcggaggat
    16741 acgctggaca atcaggagct atccgtcatg gtatcgctcg tgcattgcta gaagttgatc
    16801 ctgacttccg tttagctctt aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg
    16861 aacgtaaaaa accaggtctt aagaaagcac gtaaagcttc acaattctca aaacgttaat
    16921 atcctttata tatcaacgtt tctggcactt ttcaattatt tggaaagtgc ttcttttgtt
    16981 tttaagaaca catttaagaa cacatatcaa tcacacttat tcaatcctct ctgaaaagct
    17041 tcaaaaacag ccttatcaac tatatctggt gtgttcacat atatctttgt tgtagaaata
    17101 tctgagtgtg ttaaagcatt tgaaatttga ttcatatctg ctccaccttg tctagctata
    17161 gttgcataag tatgacggag tttgtgtgga gataaatgaa ctaagtcagg atgccttctt
    17221 ttaattgaat tgattcgata gtttaggtaa tcggcatgaa caggacaatt tacttctcca
    17281 gatggtttag tataagtaaa gagatactga tctaacgttt gcttaatttt taatttcaac
    17341 aattgatcag cttgtaccga tttccattca cttagcaatg tcattacaac ttctggtact
    17401 tgaatgacgg tatcttttcg accttttgta aacttttttc tttgatattt atctaaagta
    17461 tgtttaagac ggaccgtttg tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc
    17521 tcactttttc tatcaccaag atacagggta atcataaaaa gtgtgtaatc atgaaaagta
    17581 agtaaatgat tattaaaatc atcatttact gcttctatcc aattagtaag ttcttttgat
    17641 gatagtgctt gcttaccttc ttgtattctt ttttcttcta aagcattttt tttaggtgca
    17701 gtgatacttt gaataatttt agtagttc
//
LOCUS       DACDID010000049        17626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDID010000049 DACDID010000000
VERSION     DACDID010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.049"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            59..808
                     /locus_tag="IV236_001281"
     CDS             59..808
                     /locus_tag="IV236_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8771489.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IV236_001282"
     CDS             complement(1232..1402)
                     /locus_tag="IV236_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8771490.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IV236_001283"
     CDS             complement(1596..1775)
                     /locus_tag="IV236_001283"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771491.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IV236_001284"
     CDS             2162..3274
                     /locus_tag="IV236_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8771492.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IV236_001285"
     CDS             complement(3352..3645)
                     /locus_tag="IV236_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8771493.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IV236_001286"
     CDS             3813..4127
                     /locus_tag="IV236_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8771494.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IV236_001287"
     CDS             4148..6127
                     /locus_tag="IV236_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8771495.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IV236_001288"
     CDS             6140..6466
                     /locus_tag="IV236_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8771496.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IV236_001289"
     CDS             6482..7000
                     /locus_tag="IV236_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771497.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IV236_001290"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IV236_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8771498.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IV236_001291"
     CDS             complement(8436..9941)
                     /locus_tag="IV236_001291"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8771499.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IV236_001292"
     CDS             complement(10033..10623)
                     /locus_tag="IV236_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8771500.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IV236_001293"
     CDS             10802..12448
                     /locus_tag="IV236_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8771501.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IV236_001294"
     CDS             12497..13216
                     /locus_tag="IV236_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8771502.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IV236_001295"
     CDS             13695..14642
                     /locus_tag="IV236_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771503.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IV236_001296"
     CDS             14654..15574
                     /locus_tag="IV236_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771504.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IV236_001297"
     CDS             15615..17072
                     /locus_tag="IV236_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8771505.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..>17626
                     /locus_tag="IV236_001298"
     CDS             17417..>17626
                     /locus_tag="IV236_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8771506.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNF"
BASE COUNT         5542 a         3015 c         3594 g         5475 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aatttt
//
LOCUS       DACDID010000050        17523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDID010000050 DACDID010000000
VERSION     DACDID010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.050"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IV236_001299"
     CDS             162..392
                     /locus_tag="IV236_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8771507.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IV236_001300"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IV236_001300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8771508.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IV236_001301"
     CDS             1803..1979
                     /locus_tag="IV236_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8771509.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IV236_001302"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IV236_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8771510.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IV236_001303"
     CDS             2474..2941
                     /locus_tag="IV236_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771511.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IV236_001304"
     CDS             3026..3910
                     /locus_tag="IV236_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8771512.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IV236_001305"
     CDS             4027..4260
                     /locus_tag="IV236_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8771513.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IV236_001306"
     tRNA            4348..4428
                     /locus_tag="IV236_001306"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IV236_001307"
     tRNA            4435..4506
                     /locus_tag="IV236_001307"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IV236_001308"
     CDS             5021..5728
                     /locus_tag="IV236_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8771514.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IV236_001309"
     CDS             complement(5788..6432)
                     /locus_tag="IV236_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8771515.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IV236_001310"
     CDS             6559..6846
                     /locus_tag="IV236_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771516.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IV236_001311"
     CDS             6954..7274
                     /locus_tag="IV236_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8771517.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IV236_001312"
     CDS             7355..8668
                     /locus_tag="IV236_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8771518.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IV236_001313"
     CDS             8665..9384
                     /locus_tag="IV236_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8771519.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IV236_001314"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IV236_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8771520.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IV236_001315"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IV236_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8771521.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IV236_001316"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IV236_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8771522.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IV236_001317"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IV236_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8771523.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IV236_001318"
     CDS             14821..15714
                     /locus_tag="IV236_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771524.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IV236_001319"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IV236_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8771525.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5859 a         2964 c         3801 g         4899 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaa
//
LOCUS       DACDID010000051        17334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDID010000051 DACDID010000000
VERSION     DACDID010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.051"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IV236_001320"
                     /pseudo
     CDS             1..368
                     /locus_tag="IV236_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IV236_001321"
     CDS             548..1489
                     /locus_tag="IV236_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8771526.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IV236_001322"
     CDS             1558..2289
                     /locus_tag="IV236_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8771527.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IV236_001323"
     CDS             2433..4613
                     /locus_tag="IV236_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8771528.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IV236_001324"
     CDS             4633..5115
                     /locus_tag="IV236_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8771529.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IV236_001325"
     CDS             5131..5892
                     /locus_tag="IV236_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8771530.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IV236_001326"
     CDS             6023..7357
                     /locus_tag="IV236_001326"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8771531.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IV236_001327"
     CDS             7785..8270
                     /locus_tag="IV236_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8771532.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IV236_001328"
     CDS             8263..8946
                     /locus_tag="IV236_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8771533.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IV236_001329"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IV236_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8771534.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IV236_001330"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IV236_001330"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8771535.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IV236_001331"
     CDS             12562..13191
                     /locus_tag="IV236_001331"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8771536.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IV236_001332"
     CDS             13243..13575
                     /locus_tag="IV236_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8771537.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IV236_001333"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IV236_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8771538.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IV236_001334"
     CDS             14401..15774
                     /locus_tag="IV236_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8771539.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IV236_001335"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IV236_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8771540.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IV236_001336"
     CDS             16829..17269
                     /locus_tag="IV236_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8771541.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5905 a         3032 c         3815 g         4582 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taag
//
LOCUS       DACDID010000052        16875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDID010000052 DACDID010000000
VERSION     DACDID010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.052"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV236_001337"
     CDS             complement(<1..258)
                     /locus_tag="IV236_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771542.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IV236_001338"
     CDS             complement(274..924)
                     /locus_tag="IV236_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771543.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IV236_001339"
     CDS             complement(935..2107)
                     /locus_tag="IV236_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8771544.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IV236_001340"
     CDS             complement(2126..2998)
                     /locus_tag="IV236_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8771545.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IV236_001341"
     CDS             complement(3020..3940)
                     /locus_tag="IV236_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771546.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IV236_001342"
     CDS             complement(3940..4209)
                     /locus_tag="IV236_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771547.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IV236_001343"
     CDS             complement(4206..4586)
                     /locus_tag="IV236_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8771548.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="IV236_001344"
     CDS             complement(5053..6516)
                     /locus_tag="IV236_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8771549.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="IV236_001345"
     CDS             complement(6580..9597)
                     /locus_tag="IV236_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8771550.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="IV236_001346"
     CDS             complement(9748..10587)
                     /locus_tag="IV236_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8771551.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="IV236_001347"
     CDS             10732..10977
                     /locus_tag="IV236_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771552.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="IV236_001348"
     CDS             11040..11462
                     /locus_tag="IV236_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771553.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="IV236_001349"
     CDS             complement(11556..11873)
                     /locus_tag="IV236_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771554.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="IV236_001350"
     CDS             complement(11900..12106)
                     /locus_tag="IV236_001350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771555.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="IV236_001351"
     CDS             complement(12087..12269)
                     /locus_tag="IV236_001351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771556.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="IV236_001352"
     CDS             complement(12283..12633)
                     /locus_tag="IV236_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771557.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="IV236_001353"
     CDS             complement(12659..12961)
                     /locus_tag="IV236_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771558.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="IV236_001354"
     CDS             complement(12964..13197)
                     /locus_tag="IV236_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771559.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="IV236_001355"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="IV236_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="IV236_001356"
     CDS             complement(14628..15743)
                     /locus_tag="IV236_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771560.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="IV236_001357"
     CDS             complement(15749..16147)
                     /locus_tag="IV236_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771561.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4504 a         3503 c         2632 g         6236 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattc
//
LOCUS       DACDID010000053        16666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDID010000053 DACDID010000000
VERSION     DACDID010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.053"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..1066)
                     /locus_tag="IV236_001358"
     CDS             complement(140..1066)
                     /locus_tag="IV236_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8771562.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1341..2174
                     /locus_tag="IV236_001359"
     CDS             1341..2174
                     /locus_tag="IV236_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8771563.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2208..3047
                     /locus_tag="IV236_001360"
     CDS             2208..3047
                     /locus_tag="IV236_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8771564.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3073..3633
                     /locus_tag="IV236_001361"
     CDS             3073..3633
                     /locus_tag="IV236_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8771565.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3832..5553
                     /locus_tag="IV236_001362"
     CDS             3832..5553
                     /locus_tag="IV236_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8771566.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5718..6860
                     /locus_tag="IV236_001363"
     CDS             5718..6860
                     /locus_tag="IV236_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8771567.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6876..8087
                     /gene="thiI"
                     /locus_tag="IV236_001364"
     CDS             6876..8087
                     /gene="thiI"
                     /locus_tag="IV236_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8771568.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8660..9307
                     /locus_tag="IV236_001365"
     CDS             8660..9307
                     /locus_tag="IV236_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8771569.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            9700..12345
                     /locus_tag="IV236_001366"
     CDS             9700..12345
                     /locus_tag="IV236_001366"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8771570.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12655..13968
                     /locus_tag="IV236_001367"
     CDS             12655..13968
                     /locus_tag="IV236_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8771571.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13958..14611
                     /locus_tag="IV236_001368"
     CDS             13958..14611
                     /locus_tag="IV236_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8771572.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14666..15217
                     /gene="radC"
                     /locus_tag="IV236_001369"
                     /pseudo
     CDS             14666..15217
                     /gene="radC"
                     /locus_tag="IV236_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            15348..16433
                     /locus_tag="IV236_001370"
     CDS             15348..16433
                     /locus_tag="IV236_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8771573.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            16430..>16666
                     /locus_tag="IV236_001371"
     CDS             16430..>16666
                     /locus_tag="IV236_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771574.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFY"
BASE COUNT         5675 a         2777 c         3523 g         4691 t
ORIGIN      
        1 agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct caaatttttg
       61 taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct ttttaagttt
      121 agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca cccgacaaac
      181 catagtttac agcaatcaat agttttaaac gtgctttttg actattaata ctgttacaga
      241 aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa acaggctctg
      301 cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca agaagctttt
      361 ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg attaccaaac
      421 cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca gcatatgctt
      481 taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt tctaaaagtt
      541 cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat gtaggtgttc
      601 ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa tggatctcat
      661 cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg acacgaatcg
      721 cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc attgccccag
      781 tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca gtttcttcta
      841 atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa gcggacaaga
      901 tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga agattgaaga
      961 tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct aataatgggt
     1021 ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg atcgttccgc
     1081 cggtgtgtag tacaagtatt ttttttcatt ttacagactt ccttacgaat aattttgcat
     1141 agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga agaataacga
     1201 atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac
     1261 ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc
     1321 aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca
     1381 aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg
     1441 aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg
     1501 aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag
     1561 actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt
     1621 cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca
     1681 aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc
     1741 ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa
     1801 gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga
     1861 atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc
     1921 atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt
     1981 tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt
     2041 ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa
     2101 atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa
     2161 tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta
     2221 ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa
     2281 aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca
     2341 gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata
     2401 atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag
     2461 tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga
     2521 gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac
     2581 tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt
     2641 ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt
     2701 atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg
     2761 agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa
     2821 aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg
     2881 cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca
     2941 tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag
     3001 aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag
     3061 gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact
     3121 tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt
     3181 gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa
     3241 caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata
     3301 cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat
     3361 atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc
     3421 gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac
     3481 cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct
     3541 aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct
     3601 gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt
     3661 tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc
     3721 tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca
     3781 gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat
     3841 aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt
     3901 agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa
     3961 gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt
     4021 gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc
     4081 tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg
     4141 ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa
     4201 caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca
     4261 ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac
     4321 gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa
     4381 gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt
     4441 ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg
     4501 ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac
     4561 gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca
     4621 aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg
     4681 gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa
     4741 cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc
     4801 aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc
     4861 tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa
     4921 atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca
     4981 gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa
     5041 gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag
     5101 atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg
     5161 cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa
     5221 ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct
     5281 ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt agatgcagca
     5341 gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg
     5401 agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca
     5461 ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa
     5521 gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga
     5581 gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt
     5641 gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca
     5701 aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc
     5761 aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc
     5821 atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt
     5881 tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact
     5941 gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag
     6001 gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag
     6061 taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa
     6121 taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc
     6181 agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag
     6241 atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt
     6301 ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga
     6361 aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa
     6421 gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag
     6481 agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt
     6541 tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt
     6601 tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac
     6661 aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat
     6721 tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg
     6781 aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga
     6841 aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc
     6901 gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac
     6961 aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc
     7021 ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa
     7081 tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga
     7141 aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta
     7201 tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag
     7261 gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc
     7321 cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag
     7381 ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg
     7441 attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt
     7501 ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca
     7561 aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag
     7621 aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt
     7681 tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat
     7741 cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga
     7801 atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag
     7861 aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg
     7921 ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt
     7981 tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag
     8041 cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa
     8101 tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc
     8161 gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa
     8221 aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt
     8281 gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc
     8341 agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg
     8401 gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttgc gaaaaagctg
     8461 ccctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt tacgtttggt
     8521 tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc acaccctttt
     8581 ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat atgtgaaatt
     8641 attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag caactgcgaa
     8701 aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta taagtagaat
     8761 caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc gcagagactt
     8821 ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt ttcttatcga
     8881 tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag taggatatgg
     8941 aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc taaatatcgt
     9001 gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag aaatgatcta
     9061 tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag caatctctac
     9121 ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa atgtaaccgc
     9181 tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg ttcaatacat
     9241 tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact cagaagtttt
     9301 ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta attctttcaa
     9361 aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact aaaaaaaaaa
     9421 accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat tcattcttag
     9481 agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc ttgggagttt
     9541 ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg agaggcaata
     9601 atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag aatacgaaag
     9661 tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga aaataaccta
     9721 tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg gttagaacaa
     9781 gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt aatcccgccc
     9841 ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt gcaagatatg
     9901 ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg tatggaccat
     9961 gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg gatttctcgt
    10021 tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga agagtatgcg
    10081 agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag tagggaacgt
    10141 tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac cttatacgaa
    10201 aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc acgaacagct
    10261 ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca tatgagctat
    10321 gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga aacgatgttg
    10381 ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct catcggcaaa
    10441 aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga atatgtagat
    10501 atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa cgactttgag
    10561 gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg tacgatgaat
    10621 gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc aatcgtgcaa
    10681 gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag tgtgggtcat
    10741 tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt tgtaaaaatg
    10801 gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt ggaattcttc
    10861 cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga ttgggtaatc
    10921 tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga aacaggtgaa
    10981 atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga tcctgatgtt
    11041 ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg gccagatacg
    11101 gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg ttatgacatc
    11161 attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg cgaacgtcca
    11221 tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa gatgagtaaa
    11281 tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc agatgctctc
    11341 cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag ctatgaaaaa
    11401 atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt cgttatcatg
    11461 aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc agtggctgac
    11521 cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct atttgatcgt
    11581 tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga tttttgtgac
    11641 tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc aaaacaaacc
    11701 acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca tccaatcatg
    11761 ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc tctagtagtg
    11821 gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa agggatggaa
    11881 gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa tacaccattg
    11941 tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca atttttagtt
    12001 gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat atcaagtgag
    12061 atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat cttcttgccg
    12121 ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga actggaaaag
    12181 tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt tgtttctaac
    12241 gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt agagaagcaa
    12301 acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata gaaaaagaga
    12361 gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta caggaaagag
    12421 tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt tgtcgcatct
    12481 cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg tatcaagagt
    12541 caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg tcatactttt
    12601 atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt gttgatgacg
    12661 attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg tcctggcctt
    12721 gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt aaaaacgatc
    12781 catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag cctattagaa
    12841 gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt caatgaacgg
    12901 atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt aaaaaaatat
    12961 cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac tgaattcgag
    13021 acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt agcgattatc
    13081 gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat gctgacggga
    13141 ataacaacga ttggacttga tcataccgaa atacttggag atacaattga agaaatcgct
    13201 gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa tattgcccca
    13261 gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct ttatgtatac
    13321 ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga agtatttgaa
    13381 ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg gcaacatcaa
    13441 gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt ggaaaagctg
    13501 cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc aggaagaatg
    13561 gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac gcatgcaatg
    13621 aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa cattttgttt
    13681 tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca agtaccaaat
    13741 gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag tgaagtatca
    13801 gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt agcagatata
    13861 ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta tttcatttca
    13921 gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg cagtgatttt
    13981 tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca cacaagtagt
    14041 agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct taggtgtttc
    14101 agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca aaacaagtgt
    14161 acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc aaggagcggt
    14221 acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc agattcccaa
    14281 agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga agaacaatct
    14341 gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa aaccagaccc
    14401 agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa cattgatttt
    14461 agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg tagtcatggt
    14521 accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg ttgtttcttc
    14581 tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta tcttatttta
    14641 gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag tacctactag
    14701 ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg atcaggagct
    14761 tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga ttgctggaaa
    14821 tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc atgagttaga
    14881 agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga tcgaattagg
    14941 tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca gctacgaatt
    15001 ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg tttgtattta
    15061 tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt cgctgaatca
    15121 gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg cagcaaggat
    15181 cgttttagcg cataatcatc cttctggtaa tgttttaacc atagtataat aaataatagt
    15241 agttaaagta gtttaagagg agatattaaa gggatatttt actttaaata aaaattaaca
    15301 aggtttataa tttgtagtag ttaaagtatt taaagaggtg ggaacatgtg aaggttcaaa
    15361 ggatagaagt ggagaataag ccgtatccat tgtatttatt actagataaa gaataccagc
    15421 taatagaacc agtaatgaaa tttattaaat acttagataa tactggtaag tctcctaata
    15481 ccattaaagc atactgctat catttaaagt tgctgtacga gttcatggaa cagagaggtg
    15541 ttattcttaa tgatattaac tttgagttgt tagcagactt cgtaggttgg ttgagatatc
    15601 cttcagcatc aaatgtaatt gatcttcagt caaaaaaagc cataagagaa gaaacgacag
    15661 tgaatacaat tttaaatgta gttatgagtt ttcttgatta tttaagtaga ttaggagaat
    15721 ttaaatcaat tgatgtattt aaacaagcaa agggaagaaa tttcaaagga tttttacatc
    15781 atgttaataa gggtagatac caaaagaatg tcttaaagtt aagggttaaa aagaaacaga
    15841 taagaacatt gagatcagag gaagttaagc aaattattga tgcttgtcat acgaaaagag
    15901 ataaattaat tttaatgctt atgtatgagg gtggtttaag aatcggtgaa gtgttatcgc
    15961 ttaggcttga agatattgtc acttgggaca atcaaatcca tttaacacct agagatgtta
    16021 atgttaatga agcttatatt aaattaagga aggaaagaac aatacatgtg agtaaagaac
    16081 ttatgtcact ttatacagat tacttgatat atgagtatag tgaggaattg gagcatgatt
    16141 atgtttttat ttccttaaaa gaaggctatt ttgggaaacc actaaagtac caaagtgttc
    16201 ttgatctagt tagaagaata gttaaaagga ctggaataga atttacatca catatgcttc
    16261 gccacactca cgcaacgcag ctaattaggg aaggatggga tgttgcgttc gttcaaaaga
    16321 gattaggtca cgcacatgtt cagacaacgt taaataccta tgttcatctt tcagatcagg
    16381 atatgaaaaa tgagtttaat aaatacctcg agagaaagga gcataagaaa tgaatgcttc
    16441 tagtaaaagg aaaattatta gtcagagtga gattagcaaa aaaatagctg taatgaatga
    16501 agaaatgcag gggttttggg ctaataatag ttgggatata agaaaatgtc cacatccttc
    16561 tgccatagaa ttaagtaaga atcctgcttt aaggaatcgt tgggttcgtt ttgaacgtgt
    16621 taaaaatctg tggttaagaa cagaattgaa atatttttat ttttac
//
LOCUS       DACDID010000054        16499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDID010000054 DACDID010000000
VERSION     DACDID010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.054"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IV236_001372"
     CDS             complement(27..725)
                     /locus_tag="IV236_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8771575.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IV236_001373"
     CDS             complement(718..2070)
                     /locus_tag="IV236_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8771576.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IV236_001374"
     CDS             complement(2293..3693)
                     /locus_tag="IV236_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8771577.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IV236_001375"
     CDS             3854..4591
                     /locus_tag="IV236_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8771578.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IV236_001376"
     CDS             4716..5195
                     /locus_tag="IV236_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8771579.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IV236_001377"
     CDS             5192..5959
                     /locus_tag="IV236_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771580.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IV236_001378"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IV236_001378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8771581.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IV236_001379"
     CDS             complement(6862..7464)
                     /locus_tag="IV236_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8771582.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IV236_001380"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IV236_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IV236_001381"
     CDS             8145..8729
                     /locus_tag="IV236_001381"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8771583.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IV236_001382"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IV236_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8771584.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IV236_001383"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IV236_001383"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8771585.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IV236_001384"
     CDS             10630..10809
                     /locus_tag="IV236_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771586.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IV236_001385"
     CDS             10831..11856
                     /locus_tag="IV236_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8771587.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IV236_001386"
     CDS             12031..13275
                     /locus_tag="IV236_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8771588.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IV236_001387"
     CDS             13428..14375
                     /locus_tag="IV236_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8771589.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IV236_001388"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IV236_001388"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8771590.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IV236_001389"
     CDS             complement(15277..15519)
                     /locus_tag="IV236_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771591.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IV236_001390"
     tRNA            15640..15712
                     /locus_tag="IV236_001390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IV236_001391"
     CDS             complement(15831..16469)
                     /locus_tag="IV236_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8771592.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5624 a         2947 c         3317 g         4611 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaa
//
LOCUS       DACDID010000055        16485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDID010000055 DACDID010000000
VERSION     DACDID010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.055"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..1220
                     /locus_tag="IV236_001392"
     CDS             48..1220
                     /locus_tag="IV236_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8771593.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1207..1911
                     /locus_tag="IV236_001393"
     CDS             1207..1911
                     /locus_tag="IV236_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8771594.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1916..3076
                     /locus_tag="IV236_001394"
     CDS             1916..3076
                     /locus_tag="IV236_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8771595.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3152..3430
                     /locus_tag="IV236_001395"
     CDS             3152..3430
                     /locus_tag="IV236_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8771596.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3411..3782
                     /locus_tag="IV236_001396"
     CDS             3411..3782
                     /locus_tag="IV236_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8771597.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3798..4202
                     /locus_tag="IV236_001397"
     CDS             3798..4202
                     /locus_tag="IV236_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771598.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4199..4609
                     /locus_tag="IV236_001398"
     CDS             4199..4609
                     /locus_tag="IV236_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771599.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4670..5257
                     /locus_tag="IV236_001399"
     CDS             4670..5257
                     /locus_tag="IV236_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8771600.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5330..5797
                     /locus_tag="IV236_001400"
     CDS             5330..5797
                     /locus_tag="IV236_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8771601.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5843..6151
                     /locus_tag="IV236_001401"
     CDS             5843..6151
                     /locus_tag="IV236_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771602.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6346..13080
                     /locus_tag="IV236_001402"
     CDS             6346..13080
                     /locus_tag="IV236_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8771603.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13084..13956
                     /locus_tag="IV236_001403"
     CDS             13084..13956
                     /locus_tag="IV236_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771604.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13953..14580
                     /locus_tag="IV236_001404"
                     /pseudo
     CDS             13953..14580
                     /locus_tag="IV236_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14541..15002
                     /locus_tag="IV236_001405"
     CDS             14541..15002
                     /locus_tag="IV236_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771605.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15006..15830
                     /locus_tag="IV236_001406"
     CDS             15006..15830
                     /locus_tag="IV236_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771606.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15848..16393
                     /locus_tag="IV236_001407"
                     /pseudo
     CDS             15848..16393
                     /locus_tag="IV236_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         5889 a         2538 c         3526 g         4532 t
ORIGIN      
        1 ctagaaatag ggctttttta tttttctttg aaaggtggtg aaaacatgtg agcttatttg
       61 atatctttaa gcagtcaata agaaatgaag agccttctga gtggataccg gattttgtat
      121 atggagagga agtatctgct cgggcatacc tcaaaattat ggcaaaaaat actgttttag
      181 attttgttgc aagaactatg tcaacgttag aagttaagtt taaaaataaa gatgggacat
      241 cagactggga ttatattcta aatgttcgac ctaattctga tatgtcagca gctacatttt
      301 ggcaaaaatt tttttatcgg ctcttagatg acaacgaagt gttagtaata tttacagatg
      361 ataatcaatt gctgattgct gatgatttta gtcgaacgga gtatgccgtg tatgatgatg
      421 tttttacaaa tgtgatggta aaaaattttg tctttgaacg ttcatggaac atgtctgacg
      481 tcatttatat ggaatataat aacgaagaat tagaaaggtt tacacgaggt ttatttgaag
      541 attacgccgc gttgttcgga cggattctag aagttgcaat gcgtaacaac caaattcgtg
      601 ggtctgtatc cattgattca acgggaacgg cgaatgatga aaaaggaaaa gatggtaaga
      661 ctagatctca aagattacaa gaatatattg ataggatttg gaactctttt aaaactaact
      721 cagttgcaat tgttcccaaa gtgaaaggct ttgagtatga ggaatacacc aataaacaag
      781 gcgtatcgaa tcaatcgcta gatgaactga ataagatgaa gtcgtcttta attgatgatg
      841 ttgccaacgc cataggagta cctacggcgc ttatttatgg tgaaaaagct gaactggatt
      901 caaatattaa agcttttcgt aaattgtgta ttgctccact agttaaaaaa cttcaagacg
      961 aattggtggc aaaaataatt agtcgtcaag agtacaaaaa cggggaacgt atagaagtta
     1021 aaaaagttct ccaaaaagat ctattagagt atgctactca aattgataaa ataacatctg
     1081 gtggtgcgtt ttatattgac gaagttcgtg atgaagttga ttatgatgag ttgccaaata
     1141 atgaaggaaa aattcgtatt cgtacgaaaa actatgaaga aactgtgaag ggaggtgaga
     1201 acgacaatgc cgaaagctaa aaaagtacca tttcagttta ctaacgaaat tcagaatggt
     1261 aagcacattc tcaccttgag tggaaatgta cagaaaaaat attggcgtga tgatgatgtc
     1321 ataaacgcta aagatattcg tgactctcta gatactgtaa ccgatgatat tgtaatcaag
     1381 ttgaacagcc caggcggtga tgtgtttgaa gggattgaga tttacaatta tttaaaagat
     1441 cacccatcaa atattactgt agaagttaca gggcttgccg caagcgcagc aacgtttatt
     1501 gtcgctggtg ccaatgatgt gattatgaat gttgggactt ctttgatgat tcatgaagct
     1561 tctacatttg cttgggggaa taaacaggac atccaaaaaa cgttaaacgc attggaaacg
     1621 atcgatgatt ctatcttagc tatttattca gataagactg gccaatctgt tgataagttg
     1681 cgtgaatgga tgaatgaaga aaaatggttt acagcagatg aagctgtcaa atatggattt
     1741 gccaattctg tgaagcgtga agaaccacaa aatgaacctc aaaatattgc agcgatgatt
     1801 caagatgctg ttgccgttgc aatagcgaat ttaaaggaac ctgttactaa taaaatcgaa
     1861 caagaatcaa aacaaaaatc attaatcgca cgattgcgaa aaggagaata aagatatgtt
     1921 aaaaattaca gataaaactg cagatgcaaa aaaggcattt aatgctattt cttcgaaaga
     1981 agatgcaaca cccgaacaaa ttaatgctgc tctagaagaa tatgtaaccg ccattgcaga
     2041 agatgccggc aaacaggtgc gtgctgaata tgaagaattg aaaaatgtga cggacaaccg
     2101 tgtattagag gctcgtggaa ttcctacact aacagcagaa gaaacaaagt tttataatga
     2161 agccgtaaaa tctggaggtt tcgattctga cgtagtttgg ccagaaacga ttttggaacg
     2221 tgtttttgaa aatcttcaaa acgatcatcc tattttaggc ctcattaact ttacaccaac
     2281 tgtaggtcgt gtcaaagtaa ttagagcacg tcgtaaaggt gttgctgttt ttggaccatt
     2341 gcataaagac cttgaaggcc aactagatgc tgaatttggt gcaactgaat ttgttcaact
     2401 tgcgttgacc gcatttttct taatttctaa tgatacctta gatttaggac cacgatggat
     2461 tgaccgtttt atcaatcttt ctttaagtga agcagtacgt gatgtttggg ctaaaaaaat
     2521 tattacaggt tctggtaaag atgagccaat tggattgctt aaagatctag atggtgcggt
     2581 cacaagtggt gtatatccag ataaagctag cgcagggaca ttaacttttg cgaaagagaa
     2641 gattgttctt gaacttgcag gggtgatgaa aaaattatct aaatacacat acaaagtaga
     2701 tggtgacgat gaaggtgaag tgaaataccg ttctgttgct ggacgagtgt atctaattgt
     2761 taatcctgtt gattactggg atatcgtagc tagagttaca tttgcaaatt taaacaatgt
     2821 gtatgggact acgatgccat ttatcgctgt tgatcatatc atcgaatctg tagacgtacc
     2881 agaaagtaaa tgtattgcat acgttgataa agagtatgaa gctactcaat caagagctga
     2941 aaaagtctat gtatataaag aaacttttgc aatgaaacgc gcaactttgt atgctgtcga
     3001 catgcttggt aatggctatc caacaaataa tgatgcagca caagtatatg acttggactt
     3061 tacagcgggg gagtaatgtc ccctgcaatt ggtgagataa caccgacgac tgatggggca
     3121 gtcattaatc tttcgtaaat ggggttagag tatgaacact gaagaattta taaaagaata
     3181 taaggcccga tttcgtattt tccatacttc cgaagatgag gatatcggga aacagttaga
     3241 gagcgggttt gccgatataa aatctctaat cggcagtttt gatccttcca cttatgagaa
     3301 aggaaaagaa ttagtttatg aacgtactcg gtatctacgg aacgaatcac tagagtactt
     3361 ttacgacaat tttcaaacaa tgattatgga tgcatcaatt gatctggcag gtgctggata
     3421 tgccaattaa atcaaattat aagaagccta aaattgtagc tggtgatcta aatacgccag
     3481 ttactttttt tgagtttcaa cctaataagg gtccagaacc tggtgatgaa gaaaaaaagg
     3541 aactgtattt ttgtacagca ctagtttaca acccatcaat gaaagatcgt gacattcttg
     3601 gcacaaaaga aggcgttacg ataaaaatac gggatcccca tcaatcttat attcctacta
     3661 acaaacataa agtaaggata ttagattatc gatatggaga taaagtttgg gaaataatcg
     3721 atgtctcacc ggatttggaa gaaaattcaa ttgtaaagat agtattagaa ttaattactt
     3781 aaatttggtg ataaatgatg agtaacgtaa agtttgaagg cgttcaagaa acaatccaag
     3841 ctttagaaaa aaagtatgga gaaaagaaaa ctaagacatt aacaaaaaaa gcaatcaatg
     3901 ttggcgctga aaaagtagaa aaacagttgc aaacggacat gaccgttttt aaagacaaag
     3961 gttatacgat cgctgaggtt gttcgcaaga acgctacgta tagagattac aaggcagaag
     4021 ctgaaatcgg ttggaatggt ccacatcaac gatatagaat cattcatttg aatgaatggg
     4081 gatatacacg aaagggcaaa caaattagac cgagaggatt tggtgtaata acaaaatctc
     4141 ttaagaattc cgagctgttg tactttgatg ctgtaggaga ggaggttaaa aagagtttat
     4201 gaaagatatg cttaatatca tttacgaggc tctttgtttg aatgagtata ttcattcaat
     4261 gacctacaac gagaataccg agcaatatcg tattaaattt tatgagcaac ctgaaaccgc
     4321 cgataaaaca ggtccaatga tcacaattcg tcctgtagat acacctaacg aagcgtacca
     4381 cggaagtaac aaagagcttt ctgtggagca tatgatacag attgacgttg aatcgaaata
     4441 tagagcaaca tgcaagcaga ttcaatatga gataaaaaaa gagatgaaaa aactaggctt
     4501 tggccaaatt tccggtcaag ggttagatga atactttact gaaacaaacc gctttgtaga
     4561 cgcgcgtcgt tacgaaggaa atacaaaaat ttacgatacc gaatattaga aattaagaca
     4621 cgaaaataac gtgtcttttt ttattaccca aaattaggag gaaaatttta tgactttagt
     4681 aggattcaaa aaaatgacaa tcggggtctt tgacagcaca gggaagattc cagcagcaaa
     4741 tttatatgta attgaaggtg aacaaaacaa aggggcgact gtatctgctg aaatcagtgg
     4801 gctatcaaaa gaaccatcaa aagtttacgg atcgaacatc gcttactatg tctctcaaaa
     4861 aggaacaggc gatgtttcag ctacttttgg attgttagat ttaccaacag aagtaaatga
     4921 caaaattctt ggttacaaaa ccgatacaaa caaaatcagc ttcttgggag aagatactga
     4981 gccaccatat tgtgcaattt tgatggaatc ggaggatttg aacggcgata cagcaatgct
     5041 tgcgatgttc aaaggaaaat tcagtcgtga atctattaat cttaacacta cgacaaatga
     5101 agctttcgaa ccagaagcag aagaatatgt attttcagcg attgccaatg atgttgaagg
     5161 tgatgctaaa ggccaaacag tcgcaaaata tgttggagac gaagaagctt ctattactgg
     5221 tttgaaagag atggtatttc ctgcagcggg ggagtaacta gccccgttgt tggagcaatc
     5281 actcccacta ttaatggggc aacagttgaa ttaagttagg aggataacta tgccagatac
     5341 atttaaaatc tataaaaaaa atggtactga atttacaaaa gtcgcagagg gtgaaagtcc
     5401 actttctatt actggaattg cagcaaatac tcaagttgct aaaggagatt atcaagctac
     5461 gcgtgtggtc ggaggtcaag aatcagtaaa agtagatatt cctgcattca ccactcttcc
     5521 aatttcagtt actggtgtga cgttggataa aacgacagct gaagttgaac aaggcggaac
     5581 cttaaaatta actcctactg taacaccagc aaatgcaaca gacaaaacag gaagctggag
     5641 cagttcgaat acagctgtag ctacggttac tggaggcaac gttacagtaa aaacagacgc
     5701 tgaagttggc ggaactacag aagtttcatt tactactacg gatggcggta aagtagcaaa
     5761 atgtactatt acagttattg aaaaagcagc gggctagtct tcggactagc ttttttattt
     5821 ggaaaataat ggaggaatct aaatggcaaa agtaagaatt gaattgaaag aagaatcagg
     5881 aaagaaagtc tatgaaaaca tagacacaac agggaaagat tatcgaaaag ctttagaaac
     5941 aatcaaaaag ctaaatgaag acggagtaat gatttggaat cgtttagaca tttatttgga
     6001 ttttgctgta ggtatcttca gggatagcaa attgacagct gaacaaattc ttgatggctt
     6061 accatctgag aaggtaatga gcacattgga cgacatccta ggagaagtaa tggggattga
     6121 aagtaatcca gacccagacg caaaaaagta acacccgaag aagcagaaga aatgtactta
     6181 aacctatgca gagagttagt gaaagcggga tggtcactat ctgacataga agataattcc
     6241 tttgaaatga tgatgaaaat tgcgtgcact tcgcctaaga aagagaaaca aaaagaagtt
     6301 gatcttaaag acttcttgaa atccatgtag agaggaggta aacatatggc aaatggaaaa
     6361 cctttaggca atatgattat caaactagac ttagacagtt cagctttttc aaaaggtcta
     6421 gccggagcta aaaacgctgt taaccatcaa atgaaagcaa tgaaatcaca gatgcaagta
     6481 atgaactctt caggaaattc tctcggtgct ttacaagcaa aatacaatgg tttaggcgga
     6541 gttctacaag caaatgaaaa gcaagtagaa ttgctcacta aggcttataa ggatagtttt
     6601 gactctaacg gaaacgctac tgcttcgact gctaaatacg ctaatcaatt gaatcaagca
     6661 acagctagat cagcaagtta cgaatctcag atgaaaacaa ctgttggaca gattgctcgg
     6721 atgaaagtgg aaacggaagg gtttacgggg aaactaaagg ctaattctga tgtattgatt
     6781 aattctggga aaaaaattga agcctttggt aaaggagttt ctgggatagg atctgcccta
     6841 actgttggag taacagctcc aatagtggca ggcgcaactg ctgttactag tgcagctatt
     6901 tcatgggaaa gtgcttttgc aggtgtaaaa aagacaaatg atgaagttgt agactcgaat
     6961 ggaaacgtag tttattccta tagcgactta gagaaaggtc tgcgtaatct tgccacccaa
     7021 ttacctggta gccatcaaga aatagcaaat gtagccgaag cagccgggca attaggtata
     7081 aagactgaaa acgtagttag cttcactaaa acgatgatag acatgggtga atcgactaat
     7141 atgtcggctg aaactgcggc aacttcttta gcacgatttg ctaatattac ccaaatgtct
     7201 caaaaagact ttgataaatt aggatcagtc attgttgatc taggtaacaa ttttgctaca
     7261 accgaatcag aaatcactga aatgggattg cggttagcgg gagctggtaa acagattgga
     7321 atgagccaag gcgaaattct aggatttgct actgctctat cttctgttgg tgtcgaagct
     7381 gaagcgggtg ggtctgcctt ttcgaaggtc atgatccaga tgcaactagc tgtagaaaaa
     7441 ggatttggcg cttttgatca gctgaaacag atggcagaag aacaaggtgt tccgtgggtt
     7501 aatttagtaa atgctgtgag agacggtgga aaatcgttaa aagcagtatc agagcaaatg
     7561 ggattcactt catctgattt aaagaaaatg tataaagaag cggacaattc gaaaagttcg
     7621 cttgaaaatt ttgcgaatgt ggcaggtgtt acaagtgaac aattccagaa gttgttcaag
     7681 agtgatcctt cagaagcaat catcaagttc attcaagggc tgaaagattc agaaaaacat
     7741 ggtacttcag caatcaaagt tctggatgac atggatataa aagaagttcg actacgcgac
     7801 agtttattga gagctgcaaa tgctagtgac atattcaaag gagcaattga aaaaggaaac
     7861 ggcgcgtgga aacaaaatac agctttaaca aatgaagcta ataaaagata cgaaacaact
     7921 gaatcaaagc taaagatatt gaaaaacgaa gtgattgata cagcaatcga tcttggaggt
     7981 ccatttgtag acgcgttgcg tgatggtcta aatgtatcga aaccattgat aaaaaactta
     8041 gggaatatgg cgaaagcttt ctcagctatg agtgatgatc aacaaagaaa cattatcaaa
     8101 tgggttggat tagcagcagc agctggtcca gcgctgaagc ttttaggaac tggtacttca
     8161 gtaataggta aagttacttc aactactgga agattgacga aaagccttgt tgatttagca
     8221 gcgaaagcag cagaaaagaa ggctatggat gcctttgcat cgtcggtaac aacagctggt
     8281 gctgcagctg caaagacagc aggagctgga ggtattggcg ggttagtccc tatgatggga
     8341 aaaacagcta gtgctgcaag tactgctgcg ggcgccagcg gaataggcgc aatgacaacg
     8401 tcactaggtt tgttaggtcc tgctctactt ggtattgttg gcgttggtgg cgcactggca
     8461 gttggctatg gagcatggaa attatttggt gaagaagctt ggaattcctc gcaaagagta
     8521 aagcaatggg gaacagatgt cggtgaggag atagatagta ctcttgacgg agtacagaag
     8581 aatattgaag gtgcaaatgg tcaattcggt cttctcaaag agggttttac tgttgatgat
     8641 gcaaataata tggcggctaa tttcgcaacc gctggaacat ctttggaaac atctttaacg
     8701 ggtagaataa aggcattaga cgatgcacta aaagggctac ctcaatcagt tcaggatgcc
     8761 atgaaagaag tagctgaatc agaagaagaa accttagggc aatctctttc taaaataaaa
     8821 gacaacaatg aagaaataaa aaaaatcaga gaaagagccg caaatcagaa cagagacttg
     8881 actgtttcag aactacaaca gatacaggat ctaatgaaag gaacttctca agcgtatgtg
     8941 gatacgctcg atgtttctgc taaagacaga aaaaagatac tatcagccat gaatggtgat
     9001 gttgcacaag ctacagaaga agaagcgaag acgtggttac aatctttagc gaaacaaaaa
     9061 gctgcaacta ccgaaaatta tagacaaatg cttgaagaaa aaaaggcggc tttaaaggaa
     9121 aagggagtaa gcaaagaatt cattgatgca ttagaagatg aaatgaatga ttacatcaaa
     9181 actacaaatc aaggattcga tagccaaata gctgctataa caaataaata tccacaattg
     9241 attaacgaag tgagtttatc aaatggtcaa ttatttagca aaacggcagc tgctatggac
     9301 aatactggtc agtatacaaa agtttttctc gaaagtaatg aaaagatttt agcccaagcg
     9361 aagaaaacat cggatgaaat agcagataat gccgaaaaaa atgcagaaca acttggtatg
     9421 gtagctgatg aggctacgca tgctggatca acttggaatg gcttagtttt agacgaaaaa
     9481 acaggaaaag taaaaactaa tgtacaagaa gtagtgaatg aagcaacgaa ggatttcact
     9541 aaatggaatg aattgaaacc tgttgttcat gatgcgaaac taacaacgaa tgcaaaagat
     9601 gtcatttcca tagcggcagt acaaaatggc tattgggatg atatggaatg gaaagaaaaa
     9661 gaagcattat tagaagatga gtgttctaaa aatgtataca aggctttaga aaatgctggt
     9721 aaatggaatg agttagatat tccttctaaa gaagcaatcc taacttcaaa tactcctgaa
     9781 gttatgggtg aaactctcat gaatttggga ttatgggata cttacaaact tcaagtgaaa
     9841 gatttagatt tgaatgcgta taaagtatac gatgctatcg atggatcaga agaaaaatta
     9901 aatagttggg ctcaattaga taaccccaca aaagaattgt tattagataa ctcagatttt
     9961 tctacaaaac tttttaattc cgaagaaatg tttaaacgtt ggaatcagtt atcatctaat
    10021 gcaaagtttc tttcggctaa taatactaaa tttacagagg ttcttttatc atctgacaga
    10081 atgtggaatc aatgggtaaa ccttcctgat aatgagaaaa gattacttgc agataacgaa
    10141 gatttagcta ccactgtttt atcttcagaa gaaatatacg gaagatggac tcaattacca
    10201 gataatcaaa aatatatgtt agctgacaat actgacttag caaacaaaat attcgcttca
    10261 gaagaatcat ggaatgcatg gaaagcaatt ccagacacag ttaaaaactt gcttggtaat
    10321 aatattgatt tactggctaa agtgaaagac gggactgtaa gtgtagaaga atataacaaa
    10381 gttatgccac ttcttaaaac tctatatgga gataacgtta atttaaaaga ggcaatcagt
    10441 ggggctaaaa gctctataga gaattacaac aaaaatattt ttcctagtga taaaactgca
    10501 gtaggacacg atgaggcttc acaagctgct aaagatgcat ttgaagcgtt caacatcttc
    10561 caaacaaaaa taccagataa aattactaaa actgtatcag ctgactttat aggtcctatg
    10621 ccgaaaaatg cgaaaggaac aaacttccat cctggcggag cagctatggt aaacgatcaa
    10681 aaagggccta cttatgaaga gcttataacc ttgccaagcg gagaaacttt tatacctaaa
    10741 ggacgtaatg tcgttttaga cttaccaaga ggttcgaaag tactgaacgc cactaaaact
    10801 aaacgtctag tgcctaaata tgctgatggt ataggaaaca taacgacggt ttcatctacg
    10861 ctaaacttag atgctttaat tttagctatt aacgagctaa cgacggtatt gagaactcaa
    10921 ctgatggcag gaacgactga tgctaaagat actaaactat cagatgaaac aaaagttgcc
    10981 gatccgatta ttccggacag tctttctgaa aaatcagatc agtatctagg aattggagct
    11041 caatggttaa ctaatctaat gaatggttgg aactcagcgg ttcctcaata tatgaatagt
    11101 gaaatggttt ttattacaaa ctatctcaat gcgctaaaac ttcaaaacaa tcctaattat
    11161 acgcaaggag caacatggaa taagaactta ctgaatggat ggaatagttt aactgggacc
    11221 tttattgcta caatcaattc attttgtaat caagcaatgg tcacgcttag aaactacaac
    11281 actcctatgt acaacaatgg ccgaacgtgg cagcaaaata atcttaatgg ttggaattca
    11341 ttgtatggat cattcatagc ccgtgtaaat cagcttggta acgattcgat taacaacctt
    11401 cgttctaaaa gtggtggttt ttacaatgca gggacattct tgttgcaatc tttaataaac
    11461 ggaatgaatt cgttaggtaa ttctctttct actacaatga acaatgtcgc taatacaatg
    11521 gttggtggta taggcaaagg agttaatggc gtcatatcgg gagttaacta tgtgcttaag
    11581 gaagtcgaat caagtaagaa tatcggaaac tggccaattc cacaatacgc taagggaact
    11641 gatggacatc ctggtggact tgctatgatt aatgatcaaa aaggcctagt tcacgaagaa
    11701 tatgttcaga tgccagatgg acgtagtttt attgccaaag gtagagatct tcttgttaat
    11761 ttgcctaaag gtgctcaggt cttaaatgct tctttaacaa agaaattaaa ggaacgttta
    11821 aatattccac actacgaaaa aggtattggc aatttagata ttgttgattt gttagatgat
    11881 gaaaaaagaa tgttagaatt cttaactagc aaagttgatt tttcaggcat taatgaacca
    11941 tggcttgata tgactaaatc aggaacgtca ttaatgtcca aagctgcaaa tacaatgctt
    12001 caatcaaaat taagtgaatt cttcactcat ggcaattttg atggtgcagt aaatgccaat
    12061 ggtgtttatc aatatttagt tgatgtggca cagaaagtaa tgggtaagtt ccccggactt
    12121 acagtaactt ctggatatcg agcaggagat gcctactatc acggaaaacg gcaagctatc
    12181 gacttggctt atccaggtat ttctggagat ccgagatata cagcagcagc aaattacgct
    12241 tttgaaaaat tcccttcaaa aattgcgtat gtcattacga atggacgtgt acgtgaccgc
    12301 atgggattat ctggaacaag gtcaagcgga caatggacga attggccaga tggcgatcac
    12361 ttcgatcata ttcatttaaa cggttcgatg gggtctggcg atatattcaa tggaggagtt
    12421 ggaggaagtg gtgttgcacg ctggcgttct tatgtgtcta aagcattgaa aatgaatgga
    12481 ttaccagcta cagcagcgta tataaatgcc tggatgtccc aaatccaaac ggaatctggt
    12541 ggtaatgaga aagcaatcgg cggcaatgat gggctcgccg aaggcaatgc aacaggatta
    12601 cttcaaacga aacccggaac ttttgcagct aacgcttttc ctggtcacgg aaatattatg
    12661 aacggatttg ataacatgtt agcagcaatc aattatgcta agaaacgtta cggagttgca
    12721 ggtatgttgc aagttatcgg gaaaggacat ggatatgcca atggcggctt gattaccaaa
    12781 gacggactat atcgagcagg agaaggaaat aaaccagaaa tggttattcc attgacaaga
    12841 aaaacaagag ctatcgaact tatgggtcaa gcattagcct tcctttctgg aagcaataaa
    12901 aaagaaacga aaagtgagat taattctgta gataatagac atatcctaca agcgatagaa
    12961 aaacagtctc gtatgacaaa tgatctgcta tcattattac tctcgttttt taaaagtaac
    13021 aatcgatcag ataaagaact agctttagat attcagaaaa ttttaatcag gaggacatag
    13081 tatttgagga cagtaatatt aagaaataaa ttgaatgaag aaattgattt gtccacagaa
    13141 gattattttg ctactgaaat gtcgaatcta ggttttgaag ttacaaaaga gcatattgga
    13201 caatggggga gtttcagaga aataggagag agtattgaat tgtctgaatt tcaatcatca
    13261 gttatgattt ctgttcacgg atttagagaa caacaattat atggttcatt agttgaattt
    13321 ctggctaatg gaccttttga actagaattt tcttttgatt cagaaacaat gatcaggaaa
    13381 tgtagtttga aatcattatc taaaacagaa attgattccc aaagctcatt gctaacagat
    13441 actttgaatc tatattttac ttcaagctgg tattctatta agcgtgaaaa attaatacag
    13501 aattcaactt ctctcaatac acgtggaaaa gtttactcat ttgttcgacc atatgtctac
    13561 accaaaaatg tatgggaaaa gaaaggtgta tttaagttca gtaataattc tgtatatttg
    13621 acaaatagta cagagcgaat gtctccgtta aaaataagga ttattggaga gtgctcaaat
    13681 ccatattggg aagttatcca aaactctaaa gtagtagctt cagatggcta ttttattaat
    13741 atgaaagaca cacaaatatt agaagtgtct agtttgctag aagaccaaac ggcaatttta
    13801 aaagatgtag gtggagttga atcttctgtt tatcaacaac aagattttac taaatcaaac
    13861 ttcgtccaag cgcctctagg agaattcagc gttgtttttc atgttggaca tgcaaatgta
    13921 gagattgaac tttatgagga gcgtgatcta ttttgatttt agccattacc ctctttcacc
    13981 gagatatgaa tttatataac gaacgtttaa tttcgactga gttcgaattt ggaacggatg
    14041 aaattggaga agaagtaagt aattttatta cagataaata tgtccatgta aaatcaggag
    14101 attttctact agcaaaattt attcctagcg ggaagattgc ttattttgga gtgattagct
    14161 cgcaagaaga caaaaaaata aattgcaaag gattactcag tttggccgat agcgagatac
    14221 cgactgtaaa aatatcaggt gctaattatg aagagcatat tcgacgactg attgaatact
    14281 acttattgaa cgatccaaca aaacaactaa aaaatatttt ggaggtaaaa acagaaagcg
    14341 ttacttctca ttcttatcaa gctgttgaca caaataagag acgattgaat gcatatataa
    14401 taaatgggtt caaaaaatat aatgttaaat ggtatgtcaa agagattaaa ggtaaaaaaa
    14461 tatatactgg tattcgagca gttgatgatt caatttatat taaagataat tcatctgagt
    14521 ttaatgactg ggatgttttt gtgcaatctc ctggaccagg gaacgagaac aaattattga
    14581 tcgtagataa agcaatgaaa gatatagaaa atcccttggt attgtctacg tggtatttag
    14641 atgacgaaaa taatcttacg caagattcaa cgaataagaa cattgcattg cctaccgtta
    14701 atttagtaaa tatatatgat caaactcaag aagatagacc ttcttatgaa gaggtagcta
    14761 aatcggagct taaaggcaac gcctattcac atgaaattaa agttaatgtt gtgctaaatg
    14821 ctagaaatat caatgtagaa aatatcgaaa caggaatgtt agcgactatt gtttacaaag
    14881 agcagactta caaatctgtt ctaactgcct ggcgaatatc cagcagtaaa aagaatatag
    14941 aattgacatt tggtaatgtc cgaagtcgtt ttatggatta ttttgaagat aatgggggat
    15001 aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa
    15061 atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg
    15121 atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga
    15181 ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact
    15241 caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc
    15301 ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa
    15361 aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag
    15421 ttaattctac tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc
    15481 tctctaaagg agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc
    15541 aaccttcaaa aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg
    15601 aaaacgggca agcaattgag gatagatatg taactacgcc tttttataag gagcgtataa
    15661 cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt
    15721 ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg
    15781 caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta
    15841 tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg
    15901 tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct
    15961 tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg
    16021 cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg
    16081 gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc
    16141 atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga
    16201 cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt cttcattgga
    16261 aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca
    16321 atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg aatcagataa
    16381 gcaagtaaat gatcgtattg ataaggcaaa taatcgtatt gatcaaacca atcagcaaat
    16441 cggcgatctc ggcaagctga aaaagatgta cagtaacagc attga
//
LOCUS       DACDID010000056        16065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDID010000056 DACDID010000000
VERSION     DACDID010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.056"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IV236_001408"
     CDS             226..894
                     /locus_tag="IV236_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8771607.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IV236_001409"
     CDS             complement(1094..1480)
                     /locus_tag="IV236_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771608.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IV236_001410"
     CDS             complement(1452..2834)
                     /locus_tag="IV236_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8771609.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IV236_001411"
     CDS             complement(2990..4177)
                     /locus_tag="IV236_001411"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8771610.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IV236_001412"
     CDS             complement(4184..5215)
                     /locus_tag="IV236_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8771611.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IV236_001413"
     CDS             complement(5231..6685)
                     /locus_tag="IV236_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771612.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IV236_001414"
     CDS             6860..7765
                     /locus_tag="IV236_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8771613.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IV236_001415"
     CDS             7856..8533
                     /locus_tag="IV236_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8771614.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IV236_001416"
     CDS             8814..9710
                     /locus_tag="IV236_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8771615.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IV236_001417"
     CDS             complement(9765..11135)
                     /locus_tag="IV236_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8771616.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IV236_001418"
     CDS             11373..12119
                     /locus_tag="IV236_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8771617.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IV236_001419"
     CDS             12116..12955
                     /locus_tag="IV236_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8771618.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IV236_001420"
     CDS             12956..13906
                     /locus_tag="IV236_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8771619.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IV236_001421"
     CDS             14047..14799
                     /locus_tag="IV236_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771620.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IV236_001422"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IV236_001422"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8771621.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5209 a         3007 c         3231 g         4618 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgat
//
LOCUS       DACDID010000057        15948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDID010000057 DACDID010000000
VERSION     DACDID010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.057"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..809)
                     /locus_tag="IV236_001423"
     CDS             complement(387..809)
                     /locus_tag="IV236_001423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8771622.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IV236_001424"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IV236_001424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8771623.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="IV236_001425"
     CDS             complement(2252..3154)
                     /locus_tag="IV236_001425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8771624.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="IV236_001426"
     CDS             complement(3168..4724)
                     /locus_tag="IV236_001426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8771625.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="IV236_001427"
     CDS             complement(4747..5289)
                     /locus_tag="IV236_001427"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8771626.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IV236_001428"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IV236_001428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8771627.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IV236_001429"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IV236_001429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8771628.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IV236_001430"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IV236_001430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8771629.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IV236_001431"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IV236_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10772)
                     /locus_tag="IV236_001432"
     CDS             complement(10026..10772)
                     /locus_tag="IV236_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8771630.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IV236_001433"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IV236_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8771631.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="IV236_001434"
     CDS             complement(11238..11516)
                     /locus_tag="IV236_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8771632.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="IV236_001435"
     CDS             complement(11629..12597)
                     /locus_tag="IV236_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8771633.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="IV236_001436"
     CDS             12880..13854
                     /locus_tag="IV236_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8771634.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="IV236_001437"
     CDS             13859..14434
                     /locus_tag="IV236_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8771635.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="IV236_001438"
     CDS             complement(14501..15640)
                     /locus_tag="IV236_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8771636.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3443 c         2856 g         5293 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgat
//
LOCUS       DACDID010000058        15904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDID010000058 DACDID010000000
VERSION     DACDID010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.058"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..1820
                     /locus_tag="IV236_001439"
     CDS             120..1820
                     /locus_tag="IV236_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8771637.1"
                     /translation="MFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQ
                     TVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRN
                     LRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVS
                     RIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTAL
                     VQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEA
                     SKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSS
                     IIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLS
                     REVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPIT
                     ECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKF
                     GDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL
                     "
     gene            complement(2402..3274)
                     /locus_tag="IV236_001440"
     CDS             complement(2402..3274)
                     /locus_tag="IV236_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771638.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3574..4548
                     /locus_tag="IV236_001441"
     CDS             3574..4548
                     /locus_tag="IV236_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771639.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4570..5262
                     /locus_tag="IV236_001442"
     CDS             4570..5262
                     /locus_tag="IV236_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8771640.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5228..6736
                     /locus_tag="IV236_001443"
     CDS             5228..6736
                     /locus_tag="IV236_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8771641.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            6810..7913
                     /locus_tag="IV236_001444"
     CDS             6810..7913
                     /locus_tag="IV236_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8771642.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            7916..9253
                     /locus_tag="IV236_001445"
     CDS             7916..9253
                     /locus_tag="IV236_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8771643.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9240..10118
                     /locus_tag="IV236_001446"
     CDS             9240..10118
                     /locus_tag="IV236_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8771644.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10115..11038
                     /locus_tag="IV236_001447"
     CDS             10115..11038
                     /locus_tag="IV236_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8771645.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11035..11316
                     /locus_tag="IV236_001448"
     CDS             11035..11316
                     /locus_tag="IV236_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8771646.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            11320..12888
                     /locus_tag="IV236_001449"
     CDS             11320..12888
                     /locus_tag="IV236_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8771647.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            13128..14546
                     /locus_tag="IV236_001450"
     CDS             13128..14546
                     /locus_tag="IV236_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771648.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            14558..15868
                     /locus_tag="IV236_001451"
     CDS             14558..15868
                     /locus_tag="IV236_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8771649.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT         5283 a         2604 c         3362 g         4655 t
ORIGIN      
        1 cgttgagtca actgaccgaa gaggaagttg aaaagtatca acagaagtac ataaataaca
       61 aaaataattt attttggcaa aagaaaaaca aaggaatcga aatggtgatt tctttgccaa
      121 tgtttgaaaa agaaaaatat gcattgggaa ttgctacgat agagaaacat acaatagaac
      181 aagttgttgg tgaaagtgga gaagacatac tttctattga aaacgaagtt ggcaaccttt
      241 ttactactca gacagtaaaa aataaagagc aaccaactgt attaacggac gtggatctag
      301 attacaatca gctgaaagtg ataagagaaa aagataaaac gtatatagta ctgaaatcag
      361 attataataa ttggatttac agattagaag ttgactctag tgcaattggc gcaatgatcc
      421 gcaatctgag aatcgggcta gttgcagtgt ctttaacact ggtaggactc attggtcttt
      481 tttcctactt attttcggac agatttgccc gtccgatttc tcaaatacag gaacgtctga
      541 atcttaaatc aaaaggattc agtgggaaag aactagattt ggtcgctcaa tcagtcagtc
      601 ggatcatagg agaaaaagaa gcgttaagtg cacacttagt tacccaaaag ccacagctgg
      661 aaacgctatt tgtacttagt ttatttagaa atcgagtaga aaggcgagaa ctagatcagc
      721 ggttgcagca atttggttat agttctcaaa atgactgcta ttatacagct ttggttcaaa
      781 tcgatctgtt agaagatagt cacggtgggg agcgtgattt gctactttta gcgatcaaca
      841 acatgatcac ggaaattgtg ccaaaggaaa atcgaatgct gccgatcgtt ttgaatgaag
      901 aaatgcaagc cactatttat cgaatggata aagatgatgc agaagcaagc aaaaaagtca
      961 tggagtattg ccgactcatt caaaaacgtg taaaagaata cttgaattta actatcagta
     1021 taggcatcag taaccggttc gaaacgctga atgaaagcaa gcaatcggtt gatcgggcaa
     1081 aagaagccct gtattatcgt gtaaatactg gtccaagttc aattattttt tatcaggaaa
     1141 tcgttcctgc tgctcatgaa aaaacactta ttagatatcc gatagaagaa caaaatcgat
     1201 tgtttgaagc cattcgttct ggaaatcagg aagtgaagaa tttagtacat gaattgattg
     1261 atgcattgtt tgcacaaaat aaaaatcctt tgagtcggga agtagtttcg gtccgcttga
     1321 tcaatgaact cgttcaatta ggacagttat tgggagtaga ttcaaaaaac tttgataaca
     1381 tgaaacaaat ttatgtaaaa gcactgaaca attatcatcc caaagaacta gaaaaacttg
     1441 ttttagaaca attagttctg ccaatcacag aatgctctca agcaatcact gatcatgaat
     1501 ttaaaaattt gtcggagaat attatgcata tcgtctacac agaatatgat cgtgatctgt
     1561 ctttagatgt gatcgctgat aggttgcatt ataaccctaa ctatttaagt aatgtattta
     1621 aaaaggaaac aggagaaaaa tttggtgatt ttgttcaaaa tcatcgttta gaggtagcaa
     1681 aaaaatggtt gaaagagact aacctctccg taaaagagat tgcagaaaga ttgcaatatc
     1741 gaaatccgca aaattttatt cgtttcttta aaaagaagga atcgattacg ccgggagaat
     1801 atcgaaaaaa atatttgtga atcaagaggc tgtttttctt gtgatctgat tcaagcaata
     1861 aaagaggaca gcaaaaaaca gcacttcatg ttttgctgct ggctgatttt attgccgcag
     1921 aatcaacaag ataaacgccg tttaatcaag aggctgtttt tcttgtggac tgattcaagc
     1981 aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa tttcaaggct
     2041 aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac gaataaatgg
     2101 cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg gaagctattt
     2161 ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt gagagttttt
     2221 atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt tccaggagta
     2281 gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaatgc
     2341 cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt tcagtacata
     2401 atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga acaaaaggat
     2461 aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact gtttgtgctg
     2521 gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca atgacgattt
     2581 ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa atagcagtca
     2641 tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata gacggtaaag
     2701 ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc atttttgctg
     2761 attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc atattcattg
     2821 cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct agagaacgaa
     2881 caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca acaagaaagt
     2941 tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc agtgaagttg
     3001 cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca agtgctctaa
     3061 aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta gtaggaaaaa
     3121 ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg atgatattta
     3181 aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg aaaagtgtaa
     3241 aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc tcaaaataaa
     3301 ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac gtatataatc
     3361 atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg tagtttattt
     3421 gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgctatg taagcgtttt
     3481 tacttacgat aaaaatgatt atatacgtaa ttcagatagt tatctatcat gaagtcataa
     3541 gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa acaagaacca
     3601 tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa aataattgca
     3661 ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct ttatttcttg
     3721 atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta tcaaccattt
     3781 ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt gtttacagaa
     3841 ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa tatcttcgtg
     3901 ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa caaacgattg
     3961 aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt aatcattgtt
     4021 tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt ccttgcaaca
     4081 atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac agtatatatc
     4141 ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc agcgataact
     4201 gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg cttacgcaaa
     4261 acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct gattctaaaa
     4321 atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa ttcattgaat
     4381 cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa gaatggacag
     4441 ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt tatggtaatt
     4501 ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga tagaaagaga
     4561 gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg tattttacag
     4621 ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg agtttttact
     4681 ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg ctcaaaagaa
     4741 acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg tgaaaccttg
     4801 ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct ttctttcata
     4861 cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt gctctcagca
     4921 ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt caaaatgaaa
     4981 atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat attaagtatg
     5041 gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt tatcggtggc
     5101 agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca tttggaatgg
     5161 aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac agtgaaggag
     5221 aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag gaattctagc
     5281 acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag atggaaaagc
     5341 taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca cgattgtgaa
     5401 cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg atgcttcaaa
     5461 agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg ttacacttca
     5521 aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg tattctggga
     5581 tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag atagaatcga
     5641 agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact acgctcgtgc
     5701 tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc caactaccat
     5761 ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg gaaatggtgt
     5821 ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata ccagctttaa
     5881 acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata atggcaaatt
     5941 cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa gagatctatt
     6001 tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc agcaagaaca
     6061 atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga atctgaagac
     6121 gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga tctcagatgg
     6181 tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct tgctagcatt
     6241 tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat ttgttgatga
     6301 tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa cgcattatga
     6361 attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac aagaggtgca
     6421 accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata cagaccctga
     6481 aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa tggatccttc
     6541 tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa aaatcgtcat
     6601 tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca aaaaagctgt
     6661 agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg aagcatacaa
     6721 agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt gtcccaaatc
     6781 ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa ttagcaaaaa
     6841 gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga aaatggtctt
     6901 atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc caagaaaaaa
     6961 tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat ggaacgatcc
     7021 gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag cttttctttt
     7081 tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc ctttcccagc
     7141 agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa atttatcctt
     7201 atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa tttatccaga
     7261 attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc agttataaac
     7321 atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag aaggtccaac
     7381 catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg attggctggt
     7441 atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat tgttctttgc
     7501 tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa gaaaacagcg
     7561 ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg gaagcttccg
     7621 gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg ctgaacaaag
     7681 aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa tttgttctgg
     7741 aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg ttaggcggag
     7801 cagatcagcg agatggttca tatgcttact acatcagtga accaattatt tgtaatgatc
     7861 aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta taaatatgta
     7921 tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa tacaacaagc
     7981 cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag gtgaatttct
     8041 aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag gagcggttct
     8101 gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg aaggagtcca
     8161 tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct ctgtcactgg
     8221 tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa atgaaccaga
     8281 taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc gaattactgt
     8341 gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc tttgcagtaa
     8401 tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata cagacggaat
     8461 tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg tgggagatga
     8521 ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt gcgaaaatat
     8581 cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag gaagtgaaat
     8641 gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa ctgatcgagg
     8701 aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc gagtatccaa
     8761 tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt tctgtggtcc
     8821 gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg aacggacatc
     8881 agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg cttctgcagg
     8941 ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca atatcgatgt
     9001 ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa tagaggaaat
     9061 gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc agatgtcgat
     9121 tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca gtattgagaa
     9181 attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa actttgctta
     9241 tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt ggaaaacgat
     9301 tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac ttaaatgaat
     9361 tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt ccgacggtcc
     9421 tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg atccatggtg
     9481 ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga tatgctgttt
     9541 ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc gagttgttca
     9601 aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat gtgatcagct
     9661 tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca acgattggtc
     9721 tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt gtcaaagttt
     9781 gtattgatct agcggcacag gtggacatcg aaacactttt gctacatcgc cgcctagatc
     9841 atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg ttagcaatcc
     9901 aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat acgatgtgct
     9961 tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa tcaatgggta
    10021 gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca caagaaatga
    10081 gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca aactgataat
    10141 tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag gaatcgatta
    10201 tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag gaagatatga
    10261 tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa cagtagttat
    10321 ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag ggactttccg
    10381 aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc acatcgaaaa
    10441 tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt caggagatcg
    10501 cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca cgggtccgtt
    10561 gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca atcctgagta
    10621 ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca tatttggtgg
    10681 ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa atcctggata
    10741 catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg aaagctgtac
    10801 cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc gtccgtatgc
    10861 aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg gttgggataa
    10921 ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata acaattatga
    10981 aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc agaaatgaaa
    11041 agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat tttctttggc
    11101 gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag ttatcagtcg
    11161 cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt acaggacatg
    11221 actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagc tggggcattc
    11281 agtttatatc gagtaagaat gagaaaagga gcctgaatca tgcagactat caaagtgaaa
    11341 aatcctattt taagaggatt caatccagat cctaatatat taagagtgaa agagcggtat
    11401 tacattattg tctcctcttt tgaatggctt ccaggaatcc gagtatatga gtcgaaaaat
    11461 ttagtcaact ggtcacatac aacggatatt ttgaccactc aagtatcttt gcaaggaaat
    11521 cctacaaatg gaagtatatg ggcgccacag atcagttatg ctgatggatt gttttatctt
    11581 gtttacacag atgtcaaaag tgcaggccgt ccgtttaaag atgtccacaa ttatttgatc
    11641 actgcatctg agatcacagg tccttggtcc gaaccaattt atttaaatag taacggcttt
    11701 gatccttctt tgttccatga tcttgaaacg ggaaaaaaat ggcttgtcaa tgaactatgg
    11761 gattatcgtc tggacacacc gaataaatcc agcggtatcg tattacaaga atatgatgca
    11821 gagaagaaga agcttgatgg tcccatctat aaaatatttg caggtacaga attagcgaaa
    11881 acggaggcac cacatttgta ttttcgtaac gggtattatt atttgatgac ggcagaaggc
    11941 ggtacaggaa gaggacattc agttacagtt tgccgagcaa agaatattac tggtccttac
    12001 gagcttgatc ctggttttcc tatgctgaca gctagtgaca aacctgcttc tacattgcaa
    12061 tgtacaggac atgggagctt agtacaaacc ccgtcaggaa gatggtacat gacatatctt
    12121 tgtaccagac cattaaacgg tgcggctatt ttgggaagag aaaccgctat tcaagaagtg
    12181 tattggacgt ccgacgattg gttaagactt aaagatagcc agacaactcc gatggaagaa
    12241 ataatgattg agacaaatga agaggtacaa caagaaaaag atcatcagtt tatggataca
    12301 tttgaaggag agctaaagaa gaactggaat gccagacgca ttttgccaaa tgctgattgg
    12361 tgtgatacaa aatgtagacc aggtttttta cgtctgattt ccggtgaatc tcttcaatct
    12421 accttcgaac atcatttgtt agctattcgg caaacggatt ttacttttga tgcggagaca
    12481 gcatttgaat acatgcctaa taattttaat cagatggctg gactggtact gtacttaaat
    12541 gaatccaatt atatttattg ttatgttact tggaatgagg acaagggaaa atgcctacgt
    12601 atgatagaat cagaaaacgg agtagcacaa atagaagatt ggacgattcc actcacagaa
    12661 aagaagactt ttttgaaagt cactgtaaag aaagagacag cacagtttca tttgaaagac
    12721 gtatcaactg aagaatggaa gaaagtaggt ccgcaaaaaa acatgtgtat actctcagga
    12781 gggtttactg gaaattttat cgggatagca gtgcatgatc taaataaaaa aagaggaagt
    12841 tatgctgatt ttgagttttt caagtatgat ggaaaagatc atctgtaatt taattcttta
    12901 ataaatgagg ttcactaact tattttcact agttagtgaa tttttttgtt tgtatatatt
    12961 gttatgtaaa aaaagttatc aatcttatgc atcaatgttt ttagattatt ttttgttttt
    13021 tatctgtcaa gataatgcaa taaaaagcca acattgtgaa tataaaaaca agaaaaaaag
    13081 agttataatt ttgaaaacgt ttactagaag aggtaggagg aattcaaatg ctttcacaaa
    13141 atgctgttac agcaaataca gtaaaaaaag aaaaaaagga atttgggatt tccgataaga
    13201 taggctacat gttcggtgat ctaggtaact gttttatttt aggtttagtc aatagtttct
    13261 tgatgattta ttatacgaat gttcttggcg tttcaggggc aatcgtaggt acgttattct
    13321 tgatttcgag aattttggat gcgtttgctg atgtgatggt tggaagatta tgtgatgtct
    13381 cagagttaac agaaactggc cgatttattc cttggatcag aaaaatgaag tatccatttt
    13441 gcttagtgac tgtcattttg tttttgccat tcgtccaaca tcttccaatg acggtgaaga
    13501 ttgcttatat ttttgtttca tacattgttt atgggatttt tctctcaaca atcaatatcc
    13561 cgtatggagc aatggcagca gcaattagct caaatcctaa tgaccgcacc tcgttgtcca
    13621 cgttccgaag cattggttct gcgattggcg gatcaactac aggattcttc attccgattt
    13681 taatgtatac ccataacaag agtggacaac aagtcgtttc agggaatcat ttcttttgga
    13741 tttcaatcgg atgtgctgca atagcatttg tcgcatatat gttaacttgt aaattgacta
    13801 ctgaaagagt aagagttgtc aaaaacgaaa aagtatctgc ttctcagttg gtaaaaggtt
    13861 tgttagctaa tcgagcactg attattttag tcattgttga cattttcatt gtgatcaatc
    13921 agattttagc tgggacgaat atgacttatt tattcaatga ttacttccat aataaacaag
    13981 caatgtctgt tgctctttta tttacttacg gaacagtagt agttttagca ccatttgcta
    14041 caaagctgac aaaattattt ggtaaaaagg aagcaagcgt atttgcgtta cttgcttctt
    14101 catgtatgta cttgctgatg tatttccttc atataacaaa tgcatgggta tatctgattc
    14161 ttttatttat cgctacttta ggatctggtt tattcaattt gatggtctgg gcattcatta
    14221 ctgacgtgat tgattatcat caatataaga caggcttaag agaagatgga acagtatatg
    14281 gagtaaattc ttttgcgaga aaagtaggac aagcatgtgc aggagcggta ggcggattta
    14341 tgctggcact cattggttac caatcgtcta caacaggtgg agccatccaa tcagttttag
    14401 tacaagaaag gatttatacg atggcaaact tgttaccagc attctgctta tttgtatccg
    14461 caatcatttt gctagtaggt tacccactga atcgttcaaa aactgtattt attggtgaag
    14521 aattgaaaaa aatcaatgaa agatagggag aaacgtattg cggacaaaaa aagggattat
    14581 caatgctttg aactttaaaa caaacgacac tcaacaagat gatacaataa tactgcaaaa
    14641 agcaatcgat caaggtgcga aagaacactt acctgtattt attccaaaag gcatttattt
    14701 agtcggagcc ctttttttaa aagataagag tcatcttatc tttgaagaag gtgcagtcct
    14761 caaggggaga acagatatcg aagcatttcc agaaatcgat acacgagtag ccggcgtaga
    14821 aatgaaatgg ccagcagcga ttttgaatgt tctctcggcg aaagatattc ttatagaagg
    14881 aaaaggaata atcgacggtc aaggagacca ttggtgggag ctgtattggg gaaaagatca
    14941 aaaaagcggg acaagagctg aatacgatca aaagggactt cgttggattg cggattatgc
    15001 tattaaacgt ccacgcgctt gtttactgta tcatgcagaa catgttgttg ttcgtgattt
    15061 gatttttcag aaatcaggtt tttggaactt gcaaatcact tattctaacg atgtattagt
    15121 agaaaaagta atcatccgca acaatgatgg accaagtaca gatgggattg atattgattc
    15181 ttcaacaaat gtgcgtgtct atgaatgtga tttggcctgt ggtgatgatt gtattgctat
    15241 taagtctggc agagatggca atggtgcaag agtcaatcga aaatcatcaa gaatcgaggt
    15301 tgctagatgt aaaatccgtt caggatacgg tgtaactatt ggtagcgaag ttagtgctgg
    15361 agttagtgac gtatatattc acgatatcaa tttttttcag tcagactgtg gtattcgaat
    15421 gaaatcttca aaagaaagag ggggagtgat cgaaaatatc agggtagaaa atctgaatat
    15481 gattgatgta caatttcctt tttcttggat aatggactgg cataatgaat acaatagaaa
    15541 aaatagcaat gaccttgctc aaatgccaga aagttggcaa gcagtggctg aagaaatacc
    15601 tgaatcaaag caaatgacga aagtcaaaga catacacatc aaaaacgtac aggcatgcct
    15661 ttctccaggt tactcacttc cttcccgagc ttttgattta gtagcatttc ctgaaaagcc
    15721 tatagaagat gtctgctttt ctcattgcca aataactgca aaagaattcg ggcgaatcga
    15781 agcagtacaa aacttatgtt tcaaaagctg tgatttatct atagaaactg gcaacacagc
    15841 tgcaaacaat acttttgaca atcgttgaaa ttataataag gtcaagctta aataatattt
    15901 tagc
//
LOCUS       DACDID010000059        15634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDID010000059 DACDID010000000
VERSION     DACDID010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.059"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IV236_001452"
     CDS             complement(<1..288)
                     /locus_tag="IV236_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771650.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IV236_001453"
     CDS             430..1548
                     /locus_tag="IV236_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8771651.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IV236_001454"
     CDS             1676..3052
                     /locus_tag="IV236_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8771652.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IV236_001455"
     CDS             3453..5033
                     /locus_tag="IV236_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8771653.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IV236_001456"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IV236_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IV236_001457"
     CDS             complement(5659..5979)
                     /locus_tag="IV236_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8771654.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IV236_001458"
     CDS             complement(6127..8361)
                     /locus_tag="IV236_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8771655.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IV236_001459"
     CDS             8840..9106
                     /locus_tag="IV236_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8771656.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IV236_001460"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IV236_001460"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8771657.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10964..12043
                     /locus_tag="IV236_001461"
     CDS             10964..12043
                     /locus_tag="IV236_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8771658.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            12060..13283
                     /locus_tag="IV236_001462"
     CDS             12060..13283
                     /locus_tag="IV236_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8771659.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            13372..14259
                     /locus_tag="IV236_001463"
     CDS             13372..14259
                     /locus_tag="IV236_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8771660.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            14345..14575
                     /locus_tag="IV236_001464"
     CDS             14345..14575
                     /locus_tag="IV236_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8771661.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14632..15597)
                     /locus_tag="IV236_001465"
     CDS             complement(14632..15597)
                     /locus_tag="IV236_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8771662.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         5065 a         2698 c         3175 g         4696 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
    10981 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
    11041 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
    11101 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
    11161 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
    11221 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
    11281 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
    11341 ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta tttccaatgg
    11401 gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca gagaatattt
    11461 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
    11521 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
    11581 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
    11641 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
    11701 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
    11761 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
    11821 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
    11881 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
    11941 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
    12001 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
    12061 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
    12121 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
    12181 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
    12241 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
    12301 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
    12361 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
    12421 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
    12481 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
    12541 cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
    12601 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
    12661 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gagaaaaata
    12721 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
    12781 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
    12841 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
    12901 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
    12961 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
    13021 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
    13081 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
    13141 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
    13201 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
    13261 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc cttgtttcta
    13321 gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa aatgaaactt
    13381 ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact agaagcagga
    13441 cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt aacagaagga
    13501 gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct ctttacaatg
    13561 gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat tttttctgca
    13621 gatatctcag gacatatttt agttgatttg actacaagta caccaagcct ggcagaaaaa
    13681 attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt atccggtggt
    13741 gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga agccgtttat
    13801 gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca tggttctgct
    13861 gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac gatgacagga
    13921 atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa agtgattgag
    13981 acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc tcgtatttta
    14041 aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt aaagattgca
    14101 ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc attgaaacta
    14161 tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt gatcaaactt
    14221 tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt ttttagagaa
    14281 aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa aaggaggacc
    14341 tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta tggtcgagtc
    14401 agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga agtgtacagt
    14461 aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc atcagcaata
    14521 cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc aataaatttt
    14581 aggataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca actaatttag
    14641 ttgcttttca gcttcttttt tgtttaacag ttcaatttca aactgttctt tttctaattt
    14701 tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca attgacgttt
    14761 caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt caccagaggt
    14821 aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct gactctcatc
    14881 tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaactgct tttgttcctg
    14941 gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac cggctttttc
    15001 gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtca gtagcttttt
    15061 atttcggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag tcatttcctg
    15121 taggtcagga agctggactc gcaaagctaa caaactaatt tcagacaacc aaggcatttg
    15181 ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac gaaaaggccc
    15241 gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa tatgtacata
    15301 attataatta ctgaaataat tcatttgtcg gttataaaaa ggacggtaat gttggatcaa
    15361 ttgacactcg agcaataaag catctaattc cgtgtctact acgacgaaat caaaatctgt
    15421 tatatgatga atcatacgta atactttttt tgaatgctgt ttattctgat gaaaatagct
    15481 gcttactcta tttttgagcc gttttgcttt accaacatag atgaccttac catctcggtc
    15541 tttcataaga tagacacccg gcagcatggg gagagttttc attttttctt ttaacatagg
    15601 atctcctttt acgctttgaa taaaaagact ataa
//
LOCUS       DACDID010000060        15612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDID010000060 DACDID010000000
VERSION     DACDID010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.060"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IV236_001466"
     CDS             138..1475
                     /locus_tag="IV236_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8771663.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IV236_001467"
     CDS             1523..3652
                     /locus_tag="IV236_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8771664.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IV236_001468"
     CDS             3627..4316
                     /locus_tag="IV236_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771665.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IV236_001469"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IV236_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IV236_001470"
     CDS             complement(5546..5740)
                     /locus_tag="IV236_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771666.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IV236_001471"
     CDS             complement(5790..6230)
                     /locus_tag="IV236_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8771667.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IV236_001472"
     CDS             complement(6234..7079)
                     /locus_tag="IV236_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8771668.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IV236_001473"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IV236_001473"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8771669.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IV236_001474"
     CDS             complement(8703..9650)
                     /locus_tag="IV236_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8771670.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IV236_001475"
     CDS             complement(9873..10223)
                     /locus_tag="IV236_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8771671.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IV236_001476"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IV236_001476"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8771672.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IV236_001477"
     CDS             11646..11966
                     /locus_tag="IV236_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8771673.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IV236_001478"
     CDS             11988..12452
                     /locus_tag="IV236_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8771674.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IV236_001479"
     CDS             12657..13262
                     /locus_tag="IV236_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8771675.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IV236_001480"
     CDS             complement(13301..14590)
                     /locus_tag="IV236_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8771676.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IV236_001481"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IV236_001481"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8771677.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4879 a         2908 c         3199 g         4626 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aa
//
LOCUS       DACDID010000061        15582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDID010000061 DACDID010000000
VERSION     DACDID010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.061"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(364..730)
                     /gene="ssrA"
                     /locus_tag="IV236_001482"
     tmRNA           complement(364..730)
                     /gene="ssrA"
                     /locus_tag="IV236_001482"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(901..1674)
                     /locus_tag="IV236_001483"
     CDS             complement(901..1674)
                     /locus_tag="IV236_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8771678.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1818..2168
                     /locus_tag="IV236_001484"
     CDS             1818..2168
                     /locus_tag="IV236_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8771679.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2149..2865
                     /locus_tag="IV236_001485"
     CDS             2149..2865
                     /locus_tag="IV236_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771680.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2865..4313
                     /locus_tag="IV236_001486"
     CDS             2865..4313
                     /locus_tag="IV236_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8771681.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4383..4892)
                     /locus_tag="IV236_001487"
     CDS             complement(4383..4892)
                     /locus_tag="IV236_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771682.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4882..5607)
                     /locus_tag="IV236_001488"
     CDS             complement(4882..5607)
                     /locus_tag="IV236_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771683.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5830..7950)
                     /locus_tag="IV236_001489"
     CDS             complement(5830..7950)
                     /locus_tag="IV236_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8771684.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8180..9358
                     /locus_tag="IV236_001490"
     CDS             8180..9358
                     /locus_tag="IV236_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8771685.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9374..10132
                     /gene="aroD"
                     /locus_tag="IV236_001491"
     CDS             9374..10132
                     /gene="aroD"
                     /locus_tag="IV236_001491"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8771686.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10155..10640
                     /gene="ybaK"
                     /locus_tag="IV236_001492"
     CDS             10155..10640
                     /gene="ybaK"
                     /locus_tag="IV236_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8771687.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10711..11964)
                     /locus_tag="IV236_001493"
     CDS             complement(10711..11964)
                     /locus_tag="IV236_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8771688.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12082..13431
                     /gene="gdhA"
                     /locus_tag="IV236_001494"
     CDS             12082..13431
                     /gene="gdhA"
                     /locus_tag="IV236_001494"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8771689.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13543..14889
                     /locus_tag="IV236_001495"
     CDS             13543..14889
                     /locus_tag="IV236_001495"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8771690.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15197..>15582)
                     /locus_tag="IV236_001496"
     CDS             complement(15197..>15582)
                     /locus_tag="IV236_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8771691.1"
                     /translation="KKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAET
                     DIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKASE
                     KRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5093 a         2777 c         3143 g         4569 t
ORIGIN      
        1 ttaattgttc tagaaaaact tttgagtgaa caattatttt atgttaggat gaattaattt
       61 ctctagagtt tataattaaa aaacttattt gaaggaacat gcttaaacca taattgtgtt
      121 tttccttcca tcagcataaa agttcacctc aaaaagagtg tacgaacaaa agttcttttt
      181 gtaaacctta aaatagattt tcctgaatac gtattgggga tttctaaatg tttgaatgtt
      241 tttttgaatg caaaatcaat gcaaaaaatt ttgtatttct ctacttttct ctccttgacc
      301 aaaataaaaa aacgttgatt taacaacgtt tttcatacat gtttatctgt ctcaaccata
      361 catatatgga gacggcggga gtcgaacccg cgtccaaaca catcgccact taaaaatcta
      421 cgttcatagt caactcattt aaggattcac tttacagctt gccgagtgac aggcattctg
      481 tatcgctagt ctgatgttct ctttttcaac ttacagacgg aaagttgaaa cgtatcccac
      541 taaagttagg acccttactc gagcacatgg gcgatgccga gaggatctac gctaactgtt
      601 tttaggcagc taaagcgtaa gagttgtttt cgtttttagc agttatattt aactgtaacg
      661 tttttacgta gacgtaacct acgaaacgca attcaagctc gactgtgcct gtcgaatccg
      721 taacgtcccc gttagctaac aagggttacg tgtatttttc atacaaaatt agtatagcat
      781 atattgggat aaattcaata aaaaagcgaa tcatatccca ttatttatat aataactgcc
      841 cctttcttaa gaaaaaataa gcgattttct taagaaaaga ggtagcttca aaaaaagatc
      901 ttaaatcaga tcaaaatact tcaatgcgtg gacaattccg ccttctgtat ttttctttgt
      961 gataaaggta gaaagttctt ttaatccgtc ataggcgttg cccatagcga ttttgttgtc
     1021 gcaagcttcc aacaaggcaa aatcattcgg accatcgcca aatccaaaag taggaacttt
     1081 ttccagctga agttcttttt gcagacgttt gacacctgcc ccttttgaga ttcctttctt
     1141 cactgtatcg atcgagtaag gaccattccg ataaaaagtt agctcaggaa aatgttcgtg
     1201 atagtattcg tcacctgatt gagataagat cagcaacata tttactgtgt tttctttaaa
     1261 gcctaaagga tcaatttccg gaacatctga atgaatgaag tcgtaagcat tgatcattgt
     1321 ttggctatga ccggtacacc agattttctg atcgttatag taagaaagtt catgaccgtt
     1381 caactgtaca tgatcataca ttttttgaca ttcttctgaa gaaaatgttt gagaatagat
     1441 ttcttcgccg tctactcgaa tgaaagaacc attcatggtg accgcagaag taatgcccgc
     1501 atcttccatg atagagtgga tttctgcttc tgtacgtcct gtagcgatga cggggagaac
     1561 attgttttct tttaatgcgt tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc
     1621 atctagtaat gttccatcaa gatcaaagaa agtgattgca cgataatttg acacaaataa
     1681 aactcctttt ttattggaat aagtttatct taacaaagaa tgaacaaaag acaaaccttt
     1741 cgtgaagaat tcctgaacat attggttcag tcaacaattt agagtatact ataagaagag
     1801 aatttaggag ggaaagaatg atttttttca gtattggtat cttattctta ataattggca
     1861 gtctttttcg gattctgccc tcaaaaggaa atctgccttt ttatgggtat cattcgcctc
     1921 ttgccgcaaa aacagacgct cattggcggc tggctcaaaa gacaagcgga aactggtttt
     1981 ttcttatggg cttactgatg gcattgatcg gctattattt aaagacatct gggcatacga
     2041 attattttct gattgaaatg cttctgctag tatttccaat tatgcctatt tttattatga
     2101 cagaaaagaa attacagaaa tatgacctgg aaactggagg gaacgataat gaatatttta
     2161 atgattgaag ataatcaatc tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt
     2221 tgggaagcaa ctttcaagta tgatggaaaa gaagggctcg atgccttttt agaaaaccca
     2281 caacattggg atatgattac actagacctg aatttgccca cactggacgg tatgctagta
     2341 ggaagagaga tccgcaaagt ttcaagtact gtgccgatta tcatgctgac agctagagat
     2401 tctgaaagcg atcaggtgat cggcttagag atgggggcag atgattatgt cacaaaacct
     2461 ttcagtcctc tgacgcttat tgctcgaatg aaagctttgc atcgccgtag tgagttggca
     2521 gaagcaagag aagaagcagc agtttcagat gatcattttg atatcgagac tagtcatttc
     2581 aagatgaata cgaaaacacg agaagcgtat ctggacaacc ggccaattga aggactgacg
     2641 cctaaagagt tcgatctatt gtttacttta gcaaaaaaac cacgtcaagt attctctcgg
     2701 gagcagttgc ttgaattagt ctgggattat cagtactttg gtgatgaacg gacagtggac
     2761 gcccatatca agaaattacg acaaaaaatc gaaaaggtcg gaccacaagt catccaaact
     2821 gtttggggag tcggctacaa attcgatgat tctggtgttg cctaatgcgt tatttgtatc
     2881 agcaactctt agctttttgc ggaatggtcg cgttgatctt attgatcgtt ggagtttctt
     2941 ttacacaact gacaaaacaa acgattgaag aaaacaatta ccagcaactg tttggatatg
     3001 ctgaatcagt tgaaaagaca acgcaaacct atgccgatac gctcccgcag tttagtcaag
     3061 atcaggcgtt tcaaaatgca ttgctgctga cagagcaagt actgacagaa caaaatgtga
     3121 actttatatt tatcgataaa aacgaacgtg tcatttatcc gactaataca gctttactga
     3181 attttacctt aacagctgat caatggaaaa atctgaggaa tggacgtcaa gaaaaattca
     3241 catcgaataa aaatatttta ggacaagcac aggcgacctc ttacgcactt gtgccattta
     3301 atttgaatca tgaattctat ggagcacttg tagttagtca acctgcgcga aatatcgata
     3361 acagcgttcg tccaattaca ctgaacctgt tcaaaggatt tattttttcc agtatcgttg
     3421 cagttattgc cagctatgct tttgcagcat tccaagttaa gcgtatcaat cggttaagga
     3481 atgcgacgaa agaagtgacg aatggtaatt ttgatgtaca gctccccgta catgacaaag
     3541 atgaatttga tgagttagca gatgatttca ataaaatgac gaattcattg aaagaatctc
     3601 aagcagaaat tgaagaacaa gaaaatcgtc gaagacaatt tatggcagat gcttctcacg
     3661 agatgagaac acctcttacg acaatcaacg gacttctgga agggttagaa tacaatgcaa
     3721 tccctgaaaa tcagcgagaa aacgcgatta aattgatgaa aaatgaaaca gagcgtctga
     3781 tccgtttggt gaatgaaaac ttggattatg aaaaaatccg tacgaatcaa atcagtatgg
     3841 tcattaaaaa attcaatgga acagaaacat tgaaaaatat tgttgcacag ttagaagcta
     3901 aagcagaagc agcaggagat acattgacat tgaaagccgt tgatgatatt gatgtttacg
     3961 ctgattatga ccgatttgtc caaatcatgg tcaatattat tcaaaacgcg atacagttta
     4021 ctgaaaacgg ccaaatcacg attactttag aaaaaggcta tcttgaaacg atcatcacga
     4081 ttgaagatac gggaattgga atgtctgaac aacaaatgaa aagtatctgg gatcgttatt
     4141 ataaagtaga tccttcaaga aaaaatacaa aatatggtga atcaggatta ggtttaccaa
     4201 tcgtgcagca acttgtacgc ttgcataaag gaaaattaga agtagaaagc gaactaggaa
     4261 aaggaacaaa attcgctgtt cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa
     4321 gcggtataaa agatcatgaa tcgatcttct ataccgcttg tttttactat aaaatgattg
     4381 atttagcccg cgggctctaa acgatcaata taaggacttt gagcaatagt agcatcatga
     4441 gtaaagagta agataccttg attttcttca tgagctaaat aagaaagcaa gtggagtagc
     4501 tcttggatct ccaatacact aagttcatca aatgtatctt caataacaat attgtttcgc
     4561 ttggaaagca aggcatgaag taattgcagc tttacttttt caaaagacgt caactcagct
     4621 gccgcacaat taagcaagga aggagagata tcaagttttt cttgataggc caaacgatct
     4681 tgtttgattt ctttttccgg tacatccaac aggacatggt ctttaagatt cagataagga
     4741 aacatcggcc aatttttttg gatgatcgca aaattatcca gttttgcaac aagaacctct
     4801 tttttcaagt gctccaatat tttatgctgt ttttcgagag gacacaaaat aacggtcaag
     4861 tgttcagcat gaatcaaata ttcaggtttc attacgtcca ggcctcctac tttgcaaatg
     4921 atggataaaa aaagtgaaag ggaatagact aagaatgaga cagcatccac tgagcccaat
     4981 aagagtttgg gcaaatccct taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat
     5041 ggtaaaaaaa gtctgttgat ccatcggaat tgtaagtccg cttaccgaag tcaagatatc
     5101 acggatatct ggcagttcag tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc
     5161 ttcataaact gactggaagc ttacgatcca caataaaacc aagatacaac aaagaaagag
     5221 aggaaagaac agctgccaga aaataaaaag gaataatttg gttttagaaa gaccagtcag
     5281 gcgccaattg atcaattcct gcttgttttg cctgactgaa tagtaaaaga aaaagataaa
     5341 aaggaaggca aatagaaaaa ataaactgaa tatgcctttt atataaaatc ggctagctaa
     5401 ttctaaagcc ggaagaagct ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg
     5461 gacttgattg gctaaagccg attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa
     5521 agcagaaaag cttgaaaata aacaatagag tattgtctgc tttttgtaat aaattaatcg
     5581 agcagtagca tagcggatta ttttcatact tttcacctct ataaaaagta tagaaaatga
     5641 atatgaataa agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt
     5701 gtaaaaagca agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt
     5761 gaacactgtt tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc tccttttaaa
     5821 tttattttgt cattcggaag aacttgtggt cgttccgtga agttcttggt acgtttgcgt
     5881 tttataaaga tcaacagttg attgtccgtt gtgttcacta aagacactag ttgacttgtc
     5941 accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt cataatcact
     6001 ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac cattattgat
     6061 ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa tttcattctg
     6121 tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga tcgtaccacc
     6181 gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa gctgttcatg
     6241 atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta cacctagtaa
     6301 gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga ttccgccctg
     6361 tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat tgtcgtagtt
     6421 tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc ttgaattcga
     6481 gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc cgctttcttt
     6541 caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg caaagtaacc
     6601 gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat tggtgaattt
     6661 tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt gttgcagacg
     6721 ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt attggaacga
     6781 attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc ctaaattctt
     6841 gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg cactagtgta
     6901 accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc cgcctaattg
     6961 tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg tttccgcatc
     7021 gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg tgctgttgct
     7081 gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg agaccattct catctgtcaa
     7141 tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca cattttttcc
     7201 tttagcgagc ccgaacaaat cgtcatttgg tgctgcataa tgttctttta cataattttc
     7261 tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct ggtaagtttg
     7321 aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat aatcacgaga
     7381 gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat ttcctgagaa
     7441 aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg ggcgagggtc
     7501 caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga tccaatagat
     7561 cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg ctactttccc
     7621 agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat aagtgacatt
     7681 cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat aagcaactct
     7741 agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa tagggttgat
     7801 aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa ctaaataggc
     7861 aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga gcctcttatt
     7921 caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac ttcttaatca
     7981 cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt tactagtatg
     8041 acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt ttctttaagc
     8101 ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt gggaagagaa
     8161 agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca atcgattgaa
     8221 aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag aaaccacgca
     8281 atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc taggaaagca
     8341 gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc aatctttttt
     8401 tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag atgattctac
     8461 taccgctttt cgtatattca atggagaagg agacggaatc ggcggtctga ttgttgatta
     8521 ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac atcgtgaatt
     8581 attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg agaaaattcg
     8641 atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg caccggaacc
     8701 cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag gattaatgac
     8761 tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt tcgcttctgg
     8821 gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg ccgcaatggg
     8881 aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa aaacacagga
     8941 acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta tggacgtatt
     9001 cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg tattggatcc
     9061 accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt atggggaact
     9121 ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt ctacaaatgc
     9181 tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc atgccaaaaa
     9241 tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga cgacggatac
     9301 ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata accattagaa
     9361 ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta gaccaaagat
     9421 ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag ttcttgcacg
     9481 tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag ttttttcttt
     9541 tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac tgccgttatt
     9601 atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc gtcagtatcg
     9661 cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata tcgagttgtt
     9721 caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa acattccttt
     9781 aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc tttaccgatt
     9841 gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc ccaataatga
     9901 acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg tctccatgcc
     9961 tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg gcggaacgac
    10021 gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc caggacaatt
    10081 accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt agaacaagtt
    10141 tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc cgtatggtcg
    10201 aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc cacctaagtg
    10261 cgtcatcggt cgctgaacag cttccagaaa gccaatctcg tatatttaaa acactggttg
    10321 ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc gaattgaatc
    10381 taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat ttaaaagatt
    10441 tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg aaaaaactat
    10501 ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc tcggctggca
    10561 gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg agcggacaat
    10621 ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg ggatgttgtt
    10681 tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat tcattcctgc
    10741 tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac cattgacatc
    10801 cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg ttttaggata
    10861 aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag gaaaagtttt
    10921 gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt gtttttgaga
    10981 ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa ctaacaatcg
    11041 ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt tacttttttc
    11101 tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca gagaaagtgt
    11161 cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac gcagtgcagc
    11221 cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag tcaatacacg
    11281 acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt gaagtgtttt
    11341 ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa gaggcgtcac
    11401 cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag tagatggata
    11461 agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga attccgtgcg
    11521 tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag agaatagaag
    11581 cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt ttgatttatt
    11641 tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt gtgattcaaa
    11701 gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa actttccatt
    11761 tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat tgcaccgacc
    11821 accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca agagtacgcg
    11881 tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag cgccgactac
    11941 gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca tatctgaaga
    12001 aagaaaacag aaaaaaaatg ttttgagcca aaagagaaaa tgagctaaaa tgagggaaga
    12061 taagcaaagg agcgtggaac gatgacaaac gcaacagaat atgttcaagc aattcaagaa
    12121 aagctacatc aaaaggatca agggcaagaa gaatttttac aagcaatcga tgaattcatg
    12181 cccacggtta tgtctttttt ggacactcat cccgaatata tcgaaaagaa tatcttagaa
    12241 cttttaacgg aaccagaaag agttattcaa tttcgagtgc cttggcaaga tgatgaagga
    12301 aactggcgag taaatagagg gtaccgtatc caatacaact cagctatcgg cccttataaa
    12361 ggtggattgc gcttccatcc tagtgtcaat ctaagtatct tgaaattttt ggcttttgaa
    12421 cagattttca agaatagttt aaccggtctg ccaatcggtg gaggaaaagg cggaagtgac
    12481 ttcgatccta aaggaaaatc tgataatgag atcatgcgtt tttgtcaaag tttcatgctg
    12541 gaattgtcaa aacatatcgg cccatcgatt gatgttcctg cgggagatat cggtgtagga
    12601 gctagagaaa tcggctattt gttcggcgaa tataaacgat tgaaaaaata tgatactggt
    12661 gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga tacgtacgga agctactggt
    12721 tatggattag tgtattatgt caaacatcta ttgaacgaag aaaaagattc atttaatcaa
    12781 aaaacagttt ttgtttccgg aagcgggaat gtagccattt atgcaatcga aaaagtgcaa
    12841 gagctaggcg ggaaagttgt tacatgttca gattctagcg ggtacatcta tgatccagaa
    12901 ggaatagatg tgaaactatt gaaagaagtc aaagaaatca atagacaacg tttgacggaa
    12961 tacgcaaacc aacgtccttc tgctatttat catgctggag aatctgtttg gacattgaaa
    13021 gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg aaatagacga aagcttggcc
    13081 gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag gcgctaatat gccatgtaca
    13141 gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt attgtccagg aaaagctgct
    13201 aatgctggag gagtagcagt atctgcttta gaaatgagtc agaacgtcca gcgattgact
    13261 tgggaaagag agcaagtgga tgagcagctg gatcaaatca tggaaacgat ttaccatacg
    13321 tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc tgcttcttgg agcaaatgtt
    13381 gctggatttg aaaaagttgc caaagcaatg gcagcagaag gtttagtttg aaaaatgaaa
    13441 ctttcttgga aatattgtca gcattttgtt tatttgcttg aaaaataata aaaaaaaacg
    13501 taaagtatta cctgtagagt aaacctataa ggaggagaac aaatggcaca cattcatttt
    13561 gactattcaa aagtagcacc gtttgtcaac gaacaagagt tagggtacat gcaaagccaa
    13621 gtaacagcag cccacaacga attacgggaa ggtactggcg caggaagcga cttccgcggc
    13681 tggatcgatt taccaacgaa ctatgacaaa gaagaatttg cacgtatcaa agaagcagct
    13741 aaaaaaatcc aatctgattc tgaagtatta gttgttatcg gtatcggtgg ttcttattta
    13801 ggcgctcgtg cagcgatcga cttcttgaat cacacattct tcaatttatt agatagcaac
    13861 acaagaaaag caccacaaat cttctttgcc ggaaattcaa tcagctcaac atatattgct
    13921 gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa acgttatttc aaaatctgga
    13981 acaactactg aaccagcgat tgcgttccgt gtattcaaag agcttcttgt taagaaatat
    14041 ggacaagaag aagcaaacaa acgtatctac gctacaacag ataaagcaaa aggtgcagtt
    14101 aaagtagaag cagatgcaga aggttgggaa actttcgtga ttcctgatga catcggcgga
    14161 cgtttctctg tattaactgc tgttggtttg ttaccaatcg ctgttagtgg tgcagatatc
    14221 gatgcattga tgcaaggtgc cgctgatgca agcaaagcat actcaagcga caaactagaa
    14281 gaaaacgaag cttaccaata cgcagcaatg cgcaacattc tttaccgtaa aggcaaagta
    14341 actgagttgt tgatcaacta tgaaccagga atgcaatact tctcagaatg gtggaaacaa
    14401 ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct atccttcaag cgcaaacttc
    14461 tcaactgatt tgcactcttt aggacaattc atccaagaag gacgtcgcaa tatttttgaa
    14521 acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa ttcctgaaca agcagaagat
    14581 ttagacggtc taggctactt gcaaggaaaa gaagtggact tcgtaaatac aaaagcattt
    14641 gaaggaacat tgcttgctca tacagacggt gacgtgccta acttgctagt caaaattcca
    14701 gaaatggatg cttatacatt gggttataca atgtacttct ttgaaattgc tgtaggtatt
    14761 tcaggttact tgaatggaat caacccattc gaccaaccag gtgtagaagc ttacaagaaa
    14821 aacatgttcg cacttctagg caaaccaggc tttgaagatc ttgctaaaga attgaacgaa
    14881 cgcctgtaag ataagggttt ttcgctagat ttaaataatc agtgatagaa acatgttcgc
    14941 tttaaaatga aaactggtac ccatttaggt actcaaacat actgcatcgt ttattcagaa
    15001 taaggggctg tgacaagacg tttgtcacag tcccttgttt gtatagaaat cgattgattg
    15061 ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc gaaggttaat
    15121 tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga gtgctttttt
    15181 gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag ggtaccttat
    15241 cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac tgcaagatcg
    15301 ttccttcggg gtaagcagaa ccattggcag agtcaccaga agtaatgaca tcgaaatcta
    15361 actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa cctcttccaa
    15421 ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct ttgactaccg
    15481 cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc catcctataa
    15541 aagcaatcaa tatgataacg atcaacccta aaattttttt ca
//
LOCUS       DACDID010000062        15393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDID010000062 DACDID010000000
VERSION     DACDID010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.062"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..305
                     /locus_tag="IV236_001497"
     CDS             27..305
                     /locus_tag="IV236_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771692.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            324..1532
                     /locus_tag="IV236_001498"
     CDS             324..1532
                     /locus_tag="IV236_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771693.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1680..2045)
                     /locus_tag="IV236_001499"
     CDS             complement(1680..2045)
                     /locus_tag="IV236_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8771694.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2192..2965)
                     /locus_tag="IV236_001500"
     CDS             complement(2192..2965)
                     /locus_tag="IV236_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8771695.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3139..3999)
                     /locus_tag="IV236_001501"
     CDS             complement(3139..3999)
                     /locus_tag="IV236_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8771696.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3999..5417)
                     /locus_tag="IV236_001502"
     CDS             complement(3999..5417)
                     /locus_tag="IV236_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8771697.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5474..5923)
                     /locus_tag="IV236_001503"
     CDS             complement(5474..5923)
                     /locus_tag="IV236_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8771698.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6368..6631)
                     /locus_tag="IV236_001504"
     CDS             complement(6368..6631)
                     /locus_tag="IV236_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8771699.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6730..8025)
                     /locus_tag="IV236_001505"
     CDS             complement(6730..8025)
                     /locus_tag="IV236_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8771700.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8022..9293)
                     /locus_tag="IV236_001506"
     CDS             complement(8022..9293)
                     /locus_tag="IV236_001506"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8771701.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9458..10327)
                     /locus_tag="IV236_001507"
     CDS             complement(9458..10327)
                     /locus_tag="IV236_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8771702.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10619..12229)
                     /locus_tag="IV236_001508"
     CDS             complement(10619..12229)
                     /locus_tag="IV236_001508"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8771703.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13422..14762)
                     /gene="glnA"
                     /locus_tag="IV236_001509"
     CDS             complement(13422..14762)
                     /gene="glnA"
                     /locus_tag="IV236_001509"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8771704.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14790..15182)
                     /locus_tag="IV236_001510"
     CDS             complement(14790..15182)
                     /locus_tag="IV236_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8771705.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4377 a         3276 c         2643 g         5097 t
ORIGIN      
        1 attttttaag aatagaggga aaaaacatga attactggat gaacacaatc attaatcgat
       61 tagaaacagc atatcaaaca agatttgata tgaaagcttc tttagtcttt ttaaatgatg
      121 cgtatcaaaa ctcgatcgaa ttgatcaaag cagtggatga gaatccaaca aacgaatgcg
      181 aagaattttt aaatctgttt atgtctacaa gagatttatt tattcgccaa cttgttgatc
      241 gctatccatc caattatcac gatgtagaag ttcaaataca aaaattaaaa gcatattcag
      301 cttaagggga atgtataaca tacatggaaa agaaattatt taataaatat tttattggaa
      361 tcacattgat caattttatt gtctacctga tttattatct gttgatggtc atcatggcag
      421 tcattgcgca agacacctta cacgccagct tgagccaagc aggttttgct tctgggatct
      481 atatcatcgg aacattgctg gcacgcttat tcatggggaa acagttagaa ctatttggtc
      541 ggaagtttac cttaagaggc ggcgcgatct tctatctcct ttcaacatta gcatatcttt
      601 atacgccaac tatcggggca ttgtatttag ttcgattctt gaatggattc ggctacggta
      661 cagtttctac agctacaaat gctatcgtta ctgcatatat ccctgcatct aaaaaagggg
      721 aaggcatcaa ttattatgga ttaagtacta gtttagctgc cgctgttggg ccatttttag
      781 gaatgatttt gctgaatatg actgattttc gtttcattat ctggttttca attgtactgg
      841 tcttctttgt tacgattgcc tgccttgtct ttcctgtaaa aaatattcca ttatctgaag
      901 aacaaaaaat atctcttcgc tcatggaata tcaatagttt tattgaaaag aaagcacttt
      961 tcattacagg aattgccttt ttgatgggtc tttcttattc aagtgtttta tcatttttat
     1021 cttcttatac aaaagtaatc cacttagttg ctgctgggtc atttttcttc gtcgtatatg
     1081 ctttggtcat cacatttaca cgtccattga ctggaaggat tttcgatgtc aaaggagaac
     1141 aatacgtgat gtatcctagc tatttattct taacagcagg attattttta ctgagtgtaa
     1201 cgacgaatag tgtgacattg cttatctctg gagcattagt cggcctaggt tatggtacgt
     1261 ttatgtcaaa tggacaggct gtttgtttaa aaatcgtccg cgaacatcga atcagtattg
     1321 ctttatctac ctactttatt ggtttagacc taggcttagg cgttggtcct tatttgatgg
     1381 ggatgctaaa atctcttact tcatttagag gattgtatgt gattgccgga gtcattccat
     1441 tgatatgtac agcactctat cttgttaatg gccgaaaaca tagtcatgaa gcagagggcg
     1501 aattgattga atcattgaaa aatgaaattt gatcttgatg aatagataaa aaaaaccgtt
     1561 cagatctgaa aataatctga gcggtttttt gagtaaaaat agtttatgat tattcttaat
     1621 taaacatcta tttattttta aagctttcat ttatcgataa gaatagttta actgttgatt
     1681 tattccattt tccgttcatc tggtacgatg ccttgactta gcaaccactt gtggaagaac
     1741 cattctgcca caccgatcaa tagagcagga atcaatactt tcatccaaat atcgactgta
     1801 tcattcataa aatatagata gagccacaag ataactgcag ctgaaccagc atctgcgatc
     1861 gtcgctgcga tgttccctac tttacgtaag atcaacagat cccctagcac atagatcatg
     1921 actacaataa ttaccccaac aatcgttgaa tccataaagc taacaccgta taaacctgtc
     1981 agcacgaccc acagaatcgc ccagatcatg attgccttca ctaccaacgc cttcacatgt
     2041 ttcattttct gcactccttt ctcctggata aaaaagtaag attatctaac aagataacct
     2101 tttcattatt ataccatcgg ctgagagaag cgagcaaaga taaggtccta tctaaaaatg
     2161 aggaagccac ttagaacatt cacgtaataa tttatccgta cagacattcg atatggtgtt
     2221 cctttacttt cgcatactgt tcttttgtca gttctttact tgtcaccaga taatcgattt
     2281 catgaaaagc aatggcttga tgcatgaatt tatgtgcaat tttttgctgt gtaactaaaa
     2341 cggcaagccg gtctgattgt tttctaaaag cttttttgac cattgcctct ccttcatgag
     2401 tttctgttac tccttggctg aaatctagcc cgcgacaact cattatcgct aaatccgcat
     2461 aatatgacaa tgcatcgtta taggcttttg aaccgttgat tgttgctcgt ttcggaacaa
     2521 ttgcccctcc caatacagat acttttgccg ttgtatattc actcaacata gcagctgtga
     2581 cgatgccatt cgtaatgatc tgcaaaccat caaatcttgc taggtttctg actaagtgta
     2641 aagaggttgt actggaatcc aagaataaga ccatatcatc tcgaatcaaa gaagaagctg
     2701 tatctgcaat cttacgtttt tctgtttctt cttctaattt ggtgatcttc atcaggtaag
     2761 gcacgtcaat ttttcgattg tcaataagca tggcgccacc gcgaactcta cggatcaatc
     2821 cacttttttc cagaaaaaca aggtcacgtt taatcgtggc ttcgctataa tttactaact
     2881 cgatcaaatc ggtgactctt gcaaattggt tgttttttag ataattcaca atctcctgct
     2941 gtctattctc cacttcattc aacataaaat caccccattt actttatata gtaacaggaa
     3001 aaaagaggtt gtgacagaag tgattggctt cgagaaataa gaagaaattt ttcgaaaaaa
     3061 ctacttctga acatcgtgtg tggttaagag ggtatgacaa gactattgtc ataccctctt
     3121 gaccacacat ttttattttt agtataaact agccttgcct actgtgttca aaatttccat
     3181 tttatgtttg accacttctt gtaccttttt attagctgct ggataaattg cattcggctc
     3241 gtacatcgat ggattttcac tcaatatttc tctcaaagca ttgaaaaaga cgcttttaag
     3301 atctgagctc aaattcactt tggcaactcc gtattttact gcttcgctga cttctgcgtc
     3361 cggattacct gaaccgccat gtaaaacaaa tggaagatcg atttgttggt tcaattcttt
     3421 cagtaagtcg atatttaatt ttggagttct gtttttcgga tacaagccat gcgctgttcc
     3481 aattgctaca gcaagtgtat cgattcctgt ttctttcaca aaaatcttcg cttgatcagg
     3541 atctgtataa atgattgttt cagcacctcc ttcagcagaa ccattattcc caatcgttcc
     3601 aagctcagcc tccactgaga catttaccgc atgagctaac gcaaccactt ttttcgtaat
     3661 ctgtacattg tcgtcgaatg atttcaaaga cgcatcgatc atcacggaag aatagccgtt
     3721 tcgaatcgcc cgtaagacat cttctatcgt accgccatga tccaagtgga taacgatcgg
     3781 cagtttactt gtataagcat aggctcttac tgccgcaaca aactcatcac ttaaataggc
     3841 gatttcatca ggatgaattt ctaagattac tggtgcttct aatctctctg cttcttcgat
     3901 gattgctttc atcatatcat agctgcaaat attgaaggct ggtactgcaa attgattctc
     3961 tttagccact ttcaataact ctttcattgt atataacatt acgcttccac ctcatttgtt
     4021 agattttcat ttttcgtcca cttgtcttgt tttttctctt tcactgtttt tttcaatagt
     4081 ccaagcataa aggctgtagt gactgtacct gatgcaattg ccaagacata cataggtaaa
     4141 ttgttcataa catttggaat tagcatcacc caaatccctc catgaggtgc cattgaagta
     4201 actcctactg ccatggaaat ccctgcagcg actgctgaac ctgtcatcaa actaggtatc
     4261 acgcggatcg ggtcagatac cgcaaacggt atagcacctt ctgtaatata cgaagcgcct
     4321 aaaatccagc atgattgacc tgaagtccgt tcttctttgg taaatttatg tttgaataat
     4381 aaagtggcta aagcaatacc taggggaggt gtcattcctg cagccataca agcagcaatc
     4441 ggtgcattta ctccagctga catcaaaccg atcgagaaag tcgaagtcga tttattgatc
     4501 ggcccaccca tatcagctgc catcatgatc ccgataagta atccgaacaa tagcccggat
     4561 gtatttccca agctattcag ccaatttgtc aaagcatcca acacccatgt gatcggttgt
     4621 tcaatcacaa ataccatcaa aagcccaata attaagatcg ataaaaaggg gacgacaatc
     4681 agctgataaa tagcagaaaa ttcttttttt acgctaattt tatttgccag aaaatcacag
     4741 atataccctg ctagcacgcc accaatcatt ccgccaagaa aacctgaacc acctgtagag
     4801 gccagtaatc cggcaacaat tcctggggca aaacctgctt ttccagcaat cgatgtagca
     4861 atccccgcgc ctaacgcagg aaccatcatg gcaaacgctg tatctcctcc gatccttgaa
     4921 aatgcagctg ctaacacatt atagtctgct gagttgggat cagccgctgt aatcccaaag
     4981 gcaaaactca gtgcgatgat gattccgcct gcgatcacaa atggaagcat gtaattcaca
     5041 ccattcatga aatgattata gattgtcggc tttttctcat ttgcagctgg ttctttttca
     5101 ttagacacaa cgaatacgcc aatatctctc aatgcttcgt ctattactac ttgcggttct
     5161 ttgattgctc ggcttgtaga gacacgcttt acttttttcc cggcaaatcg gtccaattcg
     5221 atttctttat ctactgcaag aatgattgca tctgcctctt tgatttcttg ttcagataac
     5281 tgattttctg tttttacacc ttgtgtttcg aattttgctt gatggtttag atttcttgct
     5341 gttacttcta atttttctgc cgacatataa gtatgagcaa ttccagtcgg gcaagcagtt
     5401 acaccaacga ttttcatgat tattttcctc ctttttcttc actatcgata ctcggacaaa
     5461 gactctacta ttttcattct tgactaatct gtctgatgat atctgctggc gtagtggctt
     5521 cttttattcc ctttctaaaa tctgcattca tcaatcgtct ggagagtgtg ctgagaatct
     5581 ctagatgcat atctgctgat ttttcaggaa ctacgatggc aaaaatatat tcaattggca
     5641 tttcatctat cccgctatag tccgttaatt tggctgtatg aaaaaaacac agtgctggtt
     5701 ttgtcacata tttgcttttt gcatggggaa tcccaaatcc atcttctatt cctgtggaga
     5761 tctcctcctc tcgtttgaat agtgccttaa cgactttttg aacatttctt accttatttg
     5821 tttttttcaa cacatcccct attcgctcaa ataatgatcc ttttgaatct atcgatggat
     5881 cgaaaacaat cagttcttct gcgatcaatt gatcaaacct cataaaaaac ctctccccca
     5941 caaaaaatgt taacgttttc ttggaactga ggtcagtata gcatcgctag ctgtaccgtt
     6001 caatattatg aatgaacaga ggaaaaagac ccatcatccg cgtgtataag tcattaaaat
     6061 gaacgatcta gctcacaaac aatcgcaagt ctataacaaa acaaaaagcc ttccttgaaa
     6121 ggaaagctta gcttctaatt ttaggtaaat ggttttttac ttcgaaataa cccctcttca
     6181 tttcgttaat ataaggtgtt tttatcaagt ggataattaa aactatatac tagtttttca
     6241 ttgttttgtg cattatgatc ctacgacttt ttcagcatca aacgaagttt taaattggca
     6301 aaaaacataa aaaaacagac tatccctatc agatagtctg ctaaacgttg agttatcagc
     6361 gtttttatta ttccgcagcg tttgcgtctt tgagaccgta ttttttgttg aaacggtcca
     6421 cacgtccgtc tgcttgtgtg aatttttgac gtccagtgta gaatggatgt gagtcagaag
     6481 ttacttcgac acggatgact gggtatgtat taccgtcttc ccattcaact gtttcttgtg
     6541 aagtttttgt tgaacctgac aagaatttga aaccagttgt tgaatccatg aatactactg
     6601 ggtggtaatc tggatggatg ttttctttca tgattttttc gctcctttgc cctgctccat
     6661 ttgctgaacc agagttgatt tgtcgattta caacattaac atcttaacat gtttcagtca
     6721 aggttgcaat tatcttttca gatttctggt cggatttttt ttaccaaagg agacatcttg
     6781 aaattctttg aaaaattgct gattgttttt tgttttacgc aaaaagcgga tcagttgttc
     6841 cgtatattct aatgaatctc cagtcatatt ttttcgaagt ttccaaattt cttctaattc
     6901 ttcacttggc atcaataatt cttcttttcg ggtacctgat tttttgatat cgatggctgg
     6961 gaagatccga cgttcagata attcacgtga aagttgtaat tctagatttc cagttccttt
     7021 gaattcttca tagatcacgt catccatacg gcttcctgta tcgactaaag cggtcgctaa
     7081 aatcgtcaaa ctgccacctt cttcgatgtt tcttgctgcc ccgaaaaatt tcttaggttt
     7141 atacaatgcg gctgggtcaa tcccgccact caatgtacgt ccgcttggtg gaacgactaa
     7201 gttataggcc cgagcaagtc tagtaatact atccatcaag ataacgacat ctcgtttatc
     7261 ttcgactaaa cgcattgcgc gttccaatac taattcagaa actcgagtat ggttttgcgg
     7321 ctgcagatca aatgtagaag ataccacgtc gcctttcacg cttcgttcta gatccgttac
     7381 ttcttccggt cgttcatcaa tcagtagaac gattaattct acttccggat agttttcggt
     7441 gataccattt gcgatttctt ttaatacaga agttttccct gcttttggcg gtgcgacgat
     7501 caatccacgc tgaccaaaac cgattggaga aaagatatcg atcattcggg tagatagtct
     7561 ttgcggtgtt gtttccagtt gaagctgtct ttgcggataa agaggtgtta aggctggaaa
     7621 atgcggacgt tcttttgctt cttccggatc ttttccatta acgctttcca cgtgcatcaa
     7681 accataataa cgttccgatt ctttaggtgg acgggctttt cctgccactt tgtccccatt
     7741 tctcaaactg aatcgtctga tttgtgaggc agaaatatag atatcttctg cacttggtcc
     7801 ataattgatt ggccgtaaaa aaccgtaacc atcttgacca acgatatcca atattccttc
     7861 catataataa aatccttgtt tctctgcttg tgcacgaatc acagctagag ataattcctt
     7921 tttattcatc ttactgtaat aaggaatttt aaattctttg gcataactat aaacttcttt
     7981 taacgttttg ttctctagtt ctgccattgt caaataatcg ctcattcagc tcccacttcc
     8041 tctatcattt caattgatgc ccctaaggct gttagtttct cgataatatg atcataaccg
     8101 cgtaagatat aatctacatt gtaaatcgtc gttgtccctt ctgccaatag tccagcaatc
     8161 actaaacaag cacctgcccg aagatcagaa gccacgactt ctgtgccgtg aagtttattt
     8221 ggaccattca cgatgatcat atttccttcc acggtaatat cagcgcccat tcgtgctagt
     8281 tccggcacat ggttcacacg tttcgcatag atcgtatcga tgatttctga agtgcctgta
     8341 gtcatcaata gtaacgcagt aagtggctgc tgcagatctg tggcaaatcc tggataagga
     8401 taggtcatga tgtttgcggg ttttaattct ttcgaggggt aaacttcgat ttcatcttcg
     8461 ctgattttca tatggacacc aatttcctgc aattttgcta taaagctttc caaatgttca
     8521 aagatcacat ttttcacttt gataccattt cctactgcag ctgctaatgc tagataggtt
     8581 cctgcttcga tgcggtcagg aatcatgaaa tgccgacagc catgtaatgt ttcgactcct
     8641 tcgatacgga tcacatctgt acccgctcct cgaacttttg cccccatatt attcagtaaa
     8701 gttgccacat ctatgatttc cggttctcgt gcagcgtttt caattactgt ctgtcctttt
     8761 gcttttacag cagctaacat cacattgatc gttgctccga ctgaaaccac gtccatgaaa
     8821 atccgattgc cacgcaatcc tttattttct gtccgtaaat acatggcacc atgttcattt
     8881 gtcacttcag cacctagagc ttcaaatcct ttgacgtgta ggtcaatcgg tctagggcct
     8941 aaataacagc cacctggcag gcctactacc gcttcgccaa atttaccaag cagtgttccc
     9001 ataaaataat aagaagcacg caaactattg atttttccgt gcggcatcgg aatcgatacg
     9061 acttctcgag gatcgatttc tagtatgttg ttggaaaaat ggacagttgc gcccatgatc
     9121 tctaaaattt caattaatga atgaacatct tgtatgtccg gcacaccttc caagatcact
     9181 ggggaatcag ctaagattgc agctggaatc aatgcgactg cactattttt tgcaccgctg
     9241 atcgttactt caccagctaa aggtcggttt cctttgatta cgattttttt catctgtttc
     9301 caccttgttt attaatcttt tttcatggat atgcttgtat tttatcatag ataaaaaaaa
     9361 gaaacgagca tggtctgccg ttttaagtaa aaaagaatgg agagcgactt caaaagtcgt
     9421 tctccattcc tatatatcgt atctgttatg tggatgctta cgctttgtta gctgaaccga
     9481 accagtcgat atgagattta gcatcttcaa cgattgctgc tttacctgga gctaataatt
     9541 tacgagggtc gaatccttta ccttctttat ctttaccagc ttcgatatat ttacgagtag
     9601 ctgcagcaaa tgataattgg aattcagtgt taacatttac ttttgagata cccattgaga
     9661 tagctttttc gatttgatct tgaggaatac ctgatccgcc atgtaatact aaaggcatgt
     9721 ctggacctac agcatcagca attgcttgta agtggtcaaa agctaatcct ttccagtttt
     9781 ctggatattg accatggatg ttaccgatac cgcaagctaa gtagtcgata cctgtagcaa
     9841 ccatttgttt gcattcttca atatcagcta attcgccgtt accgatgatt ccgtcttctt
     9901 ctccgccgat tgaaccaact tcacattcaa ctgagatacc ttttgcatgt gctttttcaa
     9961 caacgtcttt agctaatttc aagttttctt caaatggtag atgtgaacca tcgaacatta
    10021 ctgaagtata gcctaattcg atacattcca aagcagcttc gtaatcacca tggtctaagt
    10081 ggatagcaac tggaacagtg atgttcattg cttcaactaa atcagtgatt aaatctttac
    10141 agattttgta accacccatg tatttagctg cacccattga tgtttggata agtactggtg
    10201 cttgttttgc ttcagccgct tcaaggattg cttgagtcca ttccaagttg tttgtgttga
    10261 atccgccgac agcgtaaccg cctttacgag cagcttttaa aaattcagct cctgatacta
    10321 ctggcataat aaaattcctc ctaagttttg taaaactaaa tttgttaaga caattcttaa
    10381 ccaacgaccc tattttacca aacatttcgg tagttttcta ctaaaatcaa tgaaaatgaa
    10441 aattttcaag aagttttttg caaaaaataa aatatccaat agcgctttaa tcgaaaaaag
    10501 tcttttgctt tctatctttt ttcctacaaa taataacaaa tcagaaaaag agaaaagtga
    10561 caagaaaatt gcttatcccc ttctcttgct acttttctct ttctatcaag ttcaaaaatt
    10621 attttgtttc gtgatctgct aatgatgctc cgacaaatgc tttgattaat ttttgcgggc
    10681 gatttggacg agaaatcaat tctgggtgga attggcatcc cacaaagaat tttttctctg
    10741 gcagctctac gatttctact aaacggttat ctggtgagac acctgaaaag actagccctt
    10801 gttcttcaaa caattgacga tatttgttat tgaactcata gcggtgacgg tgacgttctt
    10861 gcacaacatc ggctccatca tatgcttcag ccgtcttcgt tccttttttg attttacatg
    10921 ggtataatcc aaggcgtaaa gttccaccca aattctcgat gttttcctga tcagccatta
    10981 aatcaataat attgtttgct gtatctggat tcgtttcagc tgatgacgca tcttctaatc
    11041 caacgacgtt acgtgcaaat tcgacacaag ccatctgcat accaagacaa atacctaaaa
    11101 acggaacgtc attttcgcga gcataacgaa tcgcttcgat tttcccttct actccgcgat
    11161 caccaaatcc accaggaacc aagattccat ctgcgtcttt caaacgttct tctacatttt
    11221 ctctcgttaa ttcttgtgct ttgatccaat cgatctcgat gtctgaatta taggcgaaac
    11281 cagaatgttt caatgcttca actactgaga tatatgcatc tggtaactcg acatatttac
    11341 cgacaagcgc aatccgtact tttttcttca agttcaagac acgttcttct aattctttcc
    11401 attctgtcat gtctgcttct ggtgtatcta gttttaaatg gtcgcaaaca atttgatcca
    11461 ttccttgtgc ttgtaaaact aatgggatgg agtaaagtgt atctacatct ggcgactcga
    11521 taactgcttc tggttcgaca tcacaaaatt gtgccaattt gtttttcacg ctttgagaaa
    11581 caggttgctc tgtacgtacc accaaaatat tcggttggat acctaaacca cgtagctctt
    11641 ttacactgtg ctgcgttggt ttagttttca tttctccagc agctttcaag taaggaatca
    11701 atgttgtatg gatatacata acattatctg taccgacatc tgctttcatt tgacgaagtg
    11761 cttcaaggaa tggcaaagat tctatatccc ctactgttcc ccctacttct gtaatgatca
    11821 catctgaatc agtcatttta gctgctcgca tgattttttc tttgatctca tttgtaatat
    11881 gaggaataac ttgaacagtt gctcccaagt attcgccctt acgttcttta cgcaaaactt
    11941 cagaatagat ttttcctgtc gtcacgttgg agtatttgtt caaattgatg tcaataaaac
    12001 gttcgtagtg gcccagatcc aaatctgttt cagcaccatc atctgttacg aatacttctc
    12061 cgtgctgata tgggctcatc gtccccggat ccacgttgat gtatggatca aatttttgga
    12121 tagttacttt taagccgcgg tttttcaata agcgtcctaa agaagctgca acaatccctt
    12181 ttccgattga tgaaacgaca ccgccggtca caaaaatata ttttgtcata agttcaaaaa
    12241 ctccttttta atttatgtgc aggaaaagtc tcagacaata aacaaagctc cctattcttc
    12301 aataagaata gggagccgaa atttctgtaa acttctgacc ctctaaaagg gtgcccaagg
    12361 tgtataatac aagctgacta agggaaagtc aagtactcaa atgaatacta tccgcttaca
    12421 attttttctt tttcgatatc ctttgagaaa ttatttctta agtcctttat gagtgcaaaa
    12481 aaaatttaac ccacataata agcttgatat aaacatagct aacgttaaac ctaagcaaat
    12541 cagaaaatcg ttactgaaaa attttaagtg aaataataga taaaaaaata aaacaaacat
    12601 tatgatagat aatatcccac ctaacaaaga actacttttt cctacttgca gcaacagttt
    12661 atatagctta tcgtagaaac aatctttttc tttcaccctt atttacctcc tttacgtaaa
    12721 aaacgtagtc cacagttctc ggatttttgg catatctttt ctattaaata atatcaaaaa
    12781 caaaacaatt ccactaattt tttcaaagat gacctttttt tcaacaaaag tgccttgtag
    12841 ggattcattg aaggtaacga atcacataag taaaaaataa ggagatgcga caaaagtcgt
    12901 gcctccttat ttctaaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    12961 atccatcaaa catgagaagc tgtttttgaa attatttctt gtgattcgga gttaaatggt
    13021 aggctcacaa cctctggata aacggtattc aaaagccagt ttctcggtat taagccaaat
    13081 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttaatactcg aaactaaacg
    13141 tctttttcaa aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagttc
    13201 agccaattca aaaacaagaa aagctgtttt tgcttggcta ttcttattac ttgttgcagg
    13261 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
    13321 tcaaaaaacg gcaaaatatg aggaactatt tttccgttag tttctctaat actcaggtca
    13381 aaacgctgat ttcacagcct ctaattcacg gtattcctgt tttaataaag ttctagatat
    13441 tgttctcttt cccactcgga aacagtttgt ctgaatgccg cccattcgag acgttttgct
    13501 tcaacaaaat ttgagaagat atgttgtcct aatgcgtcaa tcattacatt atccttacgc
    13561 aattctttga tagcattatg gatagttgaa ggtaagtcct ggatatgtgc agcttctctt
    13621 tcttcctcat ccattacata aatgttacgg tcaacagctg ctggcggtgt gatctgatta
    13681 cggataccgt ctagtcctgc ttgcaacaat actgccattg ccaagtatgg gtttgcagat
    13741 ggatcaactg accgcaattc caaacgtgtg gataaaccgc gagattctgg tacacgtact
    13801 aatggcgaac gattacggcc actccatgcg acataaacag gcgcttcata acctggaacg
    13861 agacgtttat aagaattgac tgttggattg caaacggctg tgtaggcacg agcatgtttc
    13921 agcaaaccac ctaagaaatg ataagctgtt tcacttagtt ccaattcacc gtctttatca
    13981 tagaaaacat ttccttcatc attgaacaaa gacatattgc aatgcatacc agaaccattg
    14041 atcccaaata acggtttcgg catgaaagtc gcgtgaagtc catgcttgcg tgcaatcgtt
    14101 ttgacaacga gcttgaaggt ttggatatta tcacaagcat ctaccacatc tgcatattta
    14161 aagtcaattt catgctgtcc tggcgctact tcatgatgtg aagcttccac ttcaaagcct
    14221 agactttcta attccaacac gatgtcacgg cggcaatttt cacctaaatc tgttggtgca
    14281 aaatcaaaat aaccaccttt atcattcaag gtcgttgtga tcccaccgtc ttcgtctaat
    14341 ttaaataaga agaattctgg ttcaggtcct aaattgaaag aggtaaaacc aaggtcgcgc
    14401 atatctttca atgctcgttt caaattcccg cgaggatcgc cggcaaatgg tgtcccatcc
    14461 ggattataga tatcacaaat taaacgagca acttttccat gagcactttc ccacgggaag
    14521 atcatccaag tggagagatc tggatacaaa tacatatcgc tttcttcaat tcgaacaaat
    14581 ccttcaatcg aagaaccgtc aaacatcatt ttattttcta atactttgtc gatctggctc
    14641 actggaactt ctacattttt gatcgtacct aagatgtctg tgaacatcaa tcgtaaaaat
    14701 cgtacgtttt cttcctcaat gattcgtttg atttcttcac ctgttatctg tttctttacc
    14761 atggtatccc tcttcctttt ttaatttatt cagtcaaaga attgtctttg caatccttgc
    14821 gactgataat gacctgtttt gcttaaccca ccttgcgcca aaatctcatc acgaaaaatt
    14881 tgtcgaacgt catgatctgt caacggcttc ttgttttttg cttcttgttt tttcgcctgt
    14941 gccatttcgt atacacgttt gatcccagcc atattcaatc cctctgaaag atagtcttta
    15001 atctcaagca acatatcaat atcattcagc gaatacatac gtcggtttcc ttcgcttcgt
    15061 tccggatgaa ctaaatcttg ttcttcataa tagcgaattt gccgagcaga caaatcagtc
    15121 agcttcataa ctgtaccaat tggaaatacg gacatagatc ggcgcaattc cttttctccc
    15181 atcagacgtc ccctttcgtt ttataagttg gctaaagtgt accaattaaa aaaaatattg
    15241 tcaactgcat atgttaggtt ttctaacatg agatctctat tttttataaa ataacaaaaa
    15301 aataagaaca caaaaaaggt atctagttga ttttcgtacc agataccttt tattttttat
    15361 tatactgtac tatcctttat tagtgttttt att
//
LOCUS       DACDID010000063        14815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDID010000063 DACDID010000000
VERSION     DACDID010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.063"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(296..1024)
                     /locus_tag="IV236_001511"
     CDS             complement(296..1024)
                     /locus_tag="IV236_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771706.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IV236_001512"
     CDS             complement(1002..2576)
                     /locus_tag="IV236_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8771707.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IV236_001513"
     CDS             2805..3743
                     /locus_tag="IV236_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771708.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IV236_001514"
     CDS             3755..4666
                     /locus_tag="IV236_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771709.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IV236_001515"
     CDS             4690..6162
                     /locus_tag="IV236_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8771710.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IV236_001516"
     CDS             6213..6824
                     /locus_tag="IV236_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8771711.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IV236_001517"
     CDS             6854..8767
                     /locus_tag="IV236_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8771712.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IV236_001518"
     CDS             8895..11876
                     /locus_tag="IV236_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8771713.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IV236_001519"
     CDS             11873..13534
                     /locus_tag="IV236_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8771714.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IV236_001520"
     CDS             13574..14506
                     /locus_tag="IV236_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8771715.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACDID010000064        14774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDID010000064 DACDID010000000
VERSION     DACDID010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.064"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IV236_001521"
     CDS             complement(147..773)
                     /locus_tag="IV236_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771716.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IV236_001522"
     CDS             complement(1068..1403)
                     /locus_tag="IV236_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8771717.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IV236_001523"
     CDS             complement(1390..1674)
                     /locus_tag="IV236_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8771718.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IV236_001524"
     CDS             complement(1730..3253)
                     /locus_tag="IV236_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8771719.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IV236_001525"
     CDS             complement(3246..4421)
                     /locus_tag="IV236_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8771720.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IV236_001526"
     CDS             complement(4425..4610)
                     /locus_tag="IV236_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771721.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IV236_001527"
     CDS             complement(4576..6270)
                     /locus_tag="IV236_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8771722.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IV236_001528"
     CDS             complement(6267..6740)
                     /locus_tag="IV236_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8771723.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IV236_001529"
     CDS             complement(6809..6964)
                     /locus_tag="IV236_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8771724.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IV236_001530"
     CDS             complement(7098..7478)
                     /locus_tag="IV236_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8771725.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IV236_001531"
     CDS             complement(7482..7697)
                     /locus_tag="IV236_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771726.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IV236_001532"
     CDS             complement(7700..8107)
                     /locus_tag="IV236_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8771727.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IV236_001533"
     CDS             complement(8373..9848)
                     /locus_tag="IV236_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8771728.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IV236_001534"
     CDS             complement(9838..10686)
                     /locus_tag="IV236_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8771729.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IV236_001535"
     CDS             complement(10723..11085)
                     /locus_tag="IV236_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771730.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IV236_001536"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IV236_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IV236_001537"
     CDS             complement(12006..12317)
                     /locus_tag="IV236_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771731.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IV236_001538"
     CDS             complement(12361..12654)
                     /locus_tag="IV236_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771732.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IV236_001539"
     CDS             12848..13495
                     /locus_tag="IV236_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8771733.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IV236_001540"
     CDS             13556..14701
                     /locus_tag="IV236_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8771734.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4264 a         3045 c         2347 g         5118 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agct
//
LOCUS       DACDID010000065        14161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDID010000065 DACDID010000000
VERSION     DACDID010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.065"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..737)
                     /locus_tag="IV236_001541"
     CDS             complement(<1..737)
                     /locus_tag="IV236_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771735.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKL"
     gene            complement(985..1821)
                     /locus_tag="IV236_001542"
     CDS             complement(985..1821)
                     /locus_tag="IV236_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8771736.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1832..2761)
                     /locus_tag="IV236_001543"
     CDS             complement(1832..2761)
                     /locus_tag="IV236_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8771737.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2953..3306
                     /locus_tag="IV236_001544"
     CDS             2953..3306
                     /locus_tag="IV236_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8771738.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3380..4036)
                     /locus_tag="IV236_001545"
     CDS             complement(3380..4036)
                     /locus_tag="IV236_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8771739.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4262..6196
                     /locus_tag="IV236_001546"
     CDS             4262..6196
                     /locus_tag="IV236_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8771740.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6282..7505)
                     /locus_tag="IV236_001547"
     CDS             complement(6282..7505)
                     /locus_tag="IV236_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8771741.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7499..9397)
                     /locus_tag="IV236_001548"
     CDS             complement(7499..9397)
                     /locus_tag="IV236_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8771742.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9391..10509)
                     /locus_tag="IV236_001549"
     CDS             complement(9391..10509)
                     /locus_tag="IV236_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8771743.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10592..11425)
                     /locus_tag="IV236_001550"
     CDS             complement(10592..11425)
                     /locus_tag="IV236_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771744.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11418..12335)
                     /locus_tag="IV236_001551"
     CDS             complement(11418..12335)
                     /locus_tag="IV236_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771745.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12562..13686)
                     /locus_tag="IV236_001552"
     CDS             complement(12562..13686)
                     /locus_tag="IV236_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8771746.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13869..>14161
                     /locus_tag="IV236_001553"
     CDS             13869..>14161
                     /locus_tag="IV236_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8771747.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITG"
BASE COUNT         4432 a         2813 c         2295 g         4621 t
ORIGIN      
        1 tttagtttat caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact
       61 atttcgttta ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa
      121 tctttacata aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg
      181 agaatctctt catctttcaa tactaggcct attttatttt tataacctgg catgctcatt
      241 agataaagga tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc
      301 cacctttttt tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt
      361 tggatgcttt ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta
      421 atctttcgat taataacagt tattgcttta ttcggatgag acgttagata aacaatttta
      481 cacttttcat tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc
      541 aaatcgatgt ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca
      601 acaaattgta taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt
      661 ctggattctt ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata
      721 aatatacgcg ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca
      781 tacaactttt caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa
      841 cacctttgaa accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca
      901 aatgaataca tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga
      961 gctatgttta tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc
     1021 agagactttc tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata
     1081 atagtctgat tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc
     1141 ttccggcata tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc
     1201 gaatcggaac aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc
     1261 atagctgatt tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc
     1321 taaatagttt tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc
     1381 attcatttgg tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta
     1441 ttgtgtctgc atctccggat cccctactga ttctttactt actgggaaat taaatccttt
     1501 ggcttctacg atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg
     1561 caaagctagc agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg
     1621 atcgtgctcg tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac
     1681 taaagccaaa agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt
     1741 gatatattga tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga
     1801 taaaatcaaa aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc
     1861 taagggcaat cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca
     1921 tcagtagatt tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg
     1981 tgccgaacag acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca
     2041 tcgtaaaggc caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta
     2101 gcataattct agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca
     2161 aataacgacc tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat
     2221 ggatcagctg attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat
     2281 ggtgattttc tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat
     2341 aggcaagcca gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg
     2401 ccatctgcca agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga
     2461 tcggagcagt cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt
     2521 gatgaacgat cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac
     2581 cagtaaaaat agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca
     2641 aaaatacttg taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa
     2701 taacgatcga taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca
     2761 tgagtttcct cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa
     2821 aaaaacaagt tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat
     2881 tgactttttc tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa
     2941 gggagaataa aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag
     3001 tcatccgttg ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt
     3061 ccacgactag aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt
     3121 gaaacaccag aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt
     3181 catcaaggaa aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat
     3241 ctactagatg aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca
     3301 aaataatcgt tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt
     3361 gttcaatcgc tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat
     3421 gcaagaaata gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat
     3481 ctgacggagc attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg
     3541 ttaactgatt gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga
     3601 tttgttggat cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta
     3661 cgatgcgccc aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac
     3721 tttgatggaa tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa
     3781 catttgtcaa ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat
     3841 cataaccata cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg
     3901 cactatctac ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat
     3961 tatttaaaat ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa
     4021 taatatgatc tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc
     4081 atcatagcag tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat
     4141 tctttattca tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga
     4201 ctactaattg aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc
     4261 catgatatta ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga
     4321 aaacatacat ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc
     4381 tggaaaatcg actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat
     4441 cgctaaaaat aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa
     4501 tgaaacagtt tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg
     4561 gatgcgcgag ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc
     4621 ttatttaaaa gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta
     4681 tgaaaatgaa attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca
     4741 gaaaatcgat actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt
     4801 gcaacaccct gacattttga ttctagatga accaacgaac catttagata tcgagacact
     4861 ttcctggctt gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga
     4921 ccgttatttt ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg
     4981 ccattacaaa ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga
     5041 atggaaggct tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg
     5101 gaaccttgtt cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa
     5161 aatggaccgg ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat
     5221 agaaaagcct tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa
     5281 tgtcacctta tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt
     5341 aggtccgaat ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt
     5401 tatccgtgga aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca
     5461 agctgatctg catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac
     5521 tccagaaaaa gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga
     5581 aaaaccaatc cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc
     5641 gatgaacaaa gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa
     5701 caaagaagta ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca
     5761 tgaccgatat ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag
     5821 caaagtctat ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat
     5881 cgccgaattg ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga
     5941 ttattaccaa agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca
     6001 ggttgaagaa gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt
     6061 cgcgccagaa aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc
     6121 tgctagacaa gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga
     6181 agaatatgaa aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc
     6241 tttttttatt ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa
     6301 ttactagtca ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta
     6361 tttaccccat tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct
     6421 tccgttgtag catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat
     6481 aagttctttt ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc
     6541 atgatcacca tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc
     6601 caataacaaa tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt
     6661 aaatcaaaat atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc
     6721 catgcttggc ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat
     6781 cttggttttc catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt
     6841 actaaataat ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt
     6901 ccagtttctt cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt
     6961 ctaccttgtt gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc
     7021 caaaaacgct gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt
     7081 cccgttattt tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga
     7141 ttagtgataa tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt
     7201 tgttgaaata aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat
     7261 ccttctgtcc aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt
     7321 gggaattgat actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa
     7381 cgctcgatct cattattcca ttctttttcc attaattctt tataacattc atttatccat
     7441 ttttctggat gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt
     7501 agcataattt tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat
     7561 caaaatcaat tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag
     7621 caaccactcc tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa
     7681 taaaagtgtt ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt
     7741 ttcgtttaat agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc
     7801 tatagttttc cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag
     7861 caaaccctgt ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct
     7921 gccagccttc aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg
     7981 tagaggcaag tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact
     8041 gtccataggt tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct
     8101 gaatccctag cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg
     8161 aaaaaccaaa ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga
     8221 aatttggccc taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga
     8281 gcaaattggc ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag
     8341 ccgtccaata ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata
     8401 atggagataa cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa
     8461 tattcaggat acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat
     8521 tgactaaccc cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg
     8581 accaaaagct tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac
     8641 catcttccgc aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata
     8701 tctggctaat gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg
     8761 caaaaggatt ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa
     8821 gataacaatt ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac
     8881 caaatttttg gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac
     8941 attcatattc aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta
     9001 attttttcca taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta
     9061 ccatacgctg gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc
     9121 cagcttttat tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg
     9181 gccccgctcc ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc
     9241 ctaccccttg atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg
     9301 gttgatttat ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg
     9361 gatgtttcat tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa
     9421 tcgaagtaag ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag
     9481 ttctctatgg tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt
     9541 tgcatagtgc ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga
     9601 tcgtacagag gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc
     9661 actggtatca atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt
     9721 ttcgattttc tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt
     9781 ttttgtgtaa cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca
     9841 acaagaattc tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc
     9901 atcaaattct gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt
     9961 atctgcatgc tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca
    10021 atacagtaat ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt
    10081 ccatgctact aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg
    10141 taaccctagt tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg
    10201 taagaataaa aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa
    10261 taattgttct tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag
    10321 ctgccataaa ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat
    10381 tacgtctgta tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa
    10441 ttccgcatct atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg
    10501 ttcattcatg gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa
    10561 cgaactagat ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct
    10621 ccttaaattt agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta
    10681 aaattagcat aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt
    10741 tgataattaa gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat
    10801 attgattcca acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa
    10861 tcggtacagc gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct
    10921 ccatcagtgc tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac
    10981 tggcaatcga agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa
    11041 tgtaaaataa aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat
    11101 tgaacaacag tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag
    11161 aactggctgt agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca
    11221 agggataatt atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact
    11281 tgaaattgat tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat
    11341 ataaatatgg catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc
    11401 tgctttgtat cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat
    11461 aatataacag atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc
    11521 atttcaaaac ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca
    11581 ttggaagtcc caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct
    11641 aaaactgtgg ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc
    11701 cgaaatttcg atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga
    11761 agaccagcta aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc
    11821 attgatattc gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca
    11881 ttcaaaaatc cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca
    11941 gccaccgttg atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt
    12001 aactgtgctg aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt
    12061 gatcctgcca tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag
    12121 atagctagat aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta
    12181 aaagcaaacg agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt
    12241 gtgaccaatc cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc
    12301 tttattgtct tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta
    12361 atcaccagtt aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga
    12421 attttcaaaa tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc
    12481 atctgaataa gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag
    12541 ttcatagtat ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata
    12601 ggccaatgag ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc
    12661 tgtcagcata aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac
    12721 ataaaaactt gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat
    12781 cagtggagcc atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc
    12841 ataccagccg ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc
    12901 accgtagaaa tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt
    12961 cgcttcttta gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg
    13021 ggctagcatg ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact
    13081 accagttttt tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc
    13141 ttcaaaatct acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt
    13201 aaaatcacta gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa
    13261 tttttgatca taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata
    13321 aaaagcatct tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat
    13381 atccggcata tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc
    13441 cgaagctaca ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt
    13501 tgcagcattt tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt
    13561 ggcttttttt atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca
    13621 tgcacttaag ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt
    13681 tttcatgtct atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat
    13741 attaacggaa ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc
    13801 ttttcatttt tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag
    13861 aaaggaaagt ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac
    13921 taacctattc catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa
    13981 taaatgaaag cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc
    14041 cttccctttt attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa
    14101 cacaaacttt tatcattatc cagttttatt ttgatgagca attattagca catattacgg
    14161 g
//
LOCUS       DACDID010000066        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDID010000066 DACDID010000000
VERSION     DACDID010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.066"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IV236_001554"
     CDS             66..767
                     /locus_tag="IV236_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8771748.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IV236_001555"
     CDS             764..1885
                     /locus_tag="IV236_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8771749.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IV236_001556"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IV236_001556"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8771750.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IV236_001557"
     CDS             3264..4172
                     /locus_tag="IV236_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8771751.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IV236_001558"
     CDS             4207..4479
                     /locus_tag="IV236_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8771752.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IV236_001559"
     CDS             4490..5536
                     /locus_tag="IV236_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8771753.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IV236_001560"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IV236_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8771754.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IV236_001561"
     CDS             complement(8547..9218)
                     /locus_tag="IV236_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771755.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IV236_001562"
     CDS             complement(9211..9705)
                     /locus_tag="IV236_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8771756.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IV236_001563"
     CDS             complement(9725..10945)
                     /locus_tag="IV236_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771757.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IV236_001564"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IV236_001564"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8771758.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IV236_001565"
     CDS             complement(13021..14103)
                     /locus_tag="IV236_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8771759.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACDID010000067        13871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDID010000067 DACDID010000000
VERSION     DACDID010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.067"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(30..830)
                     /locus_tag="IV236_001566"
     CDS             complement(30..830)
                     /locus_tag="IV236_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8771760.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(827..1639)
                     /locus_tag="IV236_001567"
     CDS             complement(827..1639)
                     /locus_tag="IV236_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8771761.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IV236_001568"
     CDS             complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IV236_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8771762.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(2394..3116)
                     /locus_tag="IV236_001569"
     CDS             complement(2394..3116)
                     /locus_tag="IV236_001569"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8771763.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(3252..4133)
                     /locus_tag="IV236_001570"
     CDS             complement(3252..4133)
                     /locus_tag="IV236_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8771764.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IV236_001571"
     CDS             complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IV236_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8771765.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            5373..5852
                     /locus_tag="IV236_001572"
     CDS             5373..5852
                     /locus_tag="IV236_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8771766.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            6069..7160
                     /gene="phnW"
                     /locus_tag="IV236_001573"
     CDS             6069..7160
                     /gene="phnW"
                     /locus_tag="IV236_001573"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8771767.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            7153..7281
                     /locus_tag="IV236_001574"
     CDS             7153..7281
                     /locus_tag="IV236_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8771768.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            7285..7830
                     /locus_tag="IV236_001575"
     CDS             7285..7830
                     /locus_tag="IV236_001575"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8771769.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(8284..9975)
                     /locus_tag="IV236_001576"
     CDS             complement(8284..9975)
                     /locus_tag="IV236_001576"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8771770.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IV236_001577"
     CDS             complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IV236_001577"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8771771.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IV236_001578"
     CDS             complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IV236_001578"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8771772.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IV236_001579"
     CDS             complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IV236_001579"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8771773.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3854 a         2911 c         2470 g         4636 t
ORIGIN      
        1 aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg catgatagga
       61 aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg taagatattt
      121 cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc accaaattgt
      181 ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg attaaatagt
      241 tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact accaatacca
      301 ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt atgctgacca
      361 taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa tagaccaaat
      421 atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc tattcccaca
      481 tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt tttagaaata
      541 actgatgttc ctaaaagaat cataactata agataaaata acataaatgt acttacattc
      601 tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc accaacggcg
      661 gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag ctcgtaaact
      721 cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat ccatataatc
      781 ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat gactgatccc
      841 ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt tgataagagg
      901 cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt gtaaaataca
      961 gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct ccacttgtac
     1021 ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt aaatgcttag
     1081 caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact tcttcggcaa
     1141 tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca aaattcaata
     1201 ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct tgcgtatgtt
     1261 ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta atcaattctg
     1321 gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact tcatcttttg
     1381 gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact ccaagccttg
     1441 atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca gcaactcgtg
     1501 gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca atgtgctgag
     1561 gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat ttgttttttt
     1621 gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca ctatcttaag
     1681 tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat ttaaaaactg
     1741 aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa cagaggaagc
     1801 ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt
     1861 atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt
     1921 ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc
     1981 atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt
     2041 aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat
     2101 gacatttcca tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac
     2161 atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg
     2221 gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc
     2281 caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg
     2341 tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc
     2401 ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca
     2461 tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa
     2521 tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact
     2581 gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc
     2641 aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag
     2701 tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct
     2761 gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt
     2821 gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct
     2881 aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg
     2941 cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct
     3001 ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca
     3061 gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc
     3121 attcctccaa tattttctat tgccatttta ccatacttag atgatttcga cactctaaga
     3181 ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt
     3241 attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt
     3301 ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat
     3361 gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg
     3421 atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt
     3481 ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct
     3541 ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct
     3601 cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc
     3661 atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg
     3721 aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct
     3781 ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt
     3841 tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct
     3901 gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca
     3961 gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca
     4021 actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct
     4081 acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac
     4141 tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc
     4201 ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa
     4261 caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa
     4321 ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca
     4381 atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat
     4441 cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag
     4501 caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag
     4561 cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt
     4621 ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc
     4681 tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa
     4741 cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg
     4801 tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg
     4861 catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga
     4921 agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg
     4981 cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat
     5041 ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc
     5101 aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat
     5161 ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata
     5221 aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa
     5281 aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat
     5341 acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga
     5401 ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg
     5461 ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa
     5521 tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa
     5581 ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat
     5641 tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt
     5701 tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat
     5761 tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc
     5821 ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg
     5881 cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa
     5941 tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta
     6001 tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag
     6061 gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa
     6121 atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga
     6181 taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa
     6241 tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag
     6301 atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat
     6361 gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg
     6421 tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg
     6481 agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag
     6541 atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg
     6601 aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct
     6661 tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt
     6721 tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt
     6781 tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag
     6841 aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa
     6901 aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct
     6961 cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc
     7021 aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa
     7081 atattggcga gatctatccg gaagatattt tggaactgac agcaattatc aaagactata
     7141 aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt
     7201 ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg
     7261 actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt
     7321 ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa
     7381 aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag
     7441 cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc
     7501 aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca
     7561 aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt
     7621 cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat
     7681 gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg
     7741 ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa
     7801 aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag
     7861 ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg
     7921 acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc
     7981 aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat
     8041 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac
     8101 gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac
     8161 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
     8221 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
     8281 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
     8341 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
     8401 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
     8461 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
     8521 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
     8581 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
     8641 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
     8701 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
     8761 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
     8821 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
     8881 gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa
     8941 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
     9001 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
     9061 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
     9121 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
     9181 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
     9241 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
     9301 agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
     9361 tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg
     9421 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
     9481 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
     9541 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
     9601 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc
     9661 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
     9721 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
     9781 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
     9841 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
     9901 ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
     9961 atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa
    10021 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    10081 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    10141 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    10201 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    10261 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc
    10321 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
    10381 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
    10441 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
    10501 atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat
    10561 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
    10621 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
    10681 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
    10741 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
    10801 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
    10861 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
    10921 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
    10981 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
    11041 tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt
    11101 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
    11161 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
    11221 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
    11281 cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc
    11341 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
    11401 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
    11461 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
    11521 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
    11581 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
    11641 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
    11701 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
    11761 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    11821 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    11881 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    11941 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    12001 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    12061 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    12121 agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct
    12181 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    12241 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    12301 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    12361 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    12421 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    12481 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    12541 cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    12601 atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg
    12661 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    12721 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    12781 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    12841 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    12901 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    12961 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt
    13021 gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    13081 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    13141 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    13201 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    13261 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    13321 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    13381 tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta
    13441 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    13501 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    13561 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    13621 aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    13681 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc
    13741 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    13801 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    13861 aattttgtct c
//
LOCUS       DACDID010000068        13813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDID010000068 DACDID010000000
VERSION     DACDID010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.068"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..1462
                     /locus_tag="IV236_001580"
     CDS             38..1462
                     /locus_tag="IV236_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8771774.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1761..2468
                     /locus_tag="IV236_001581"
     CDS             1761..2468
                     /locus_tag="IV236_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8771775.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2455..2778
                     /locus_tag="IV236_001582"
     CDS             2455..2778
                     /locus_tag="IV236_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8771776.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2867..3904)
                     /locus_tag="IV236_001583"
     CDS             complement(2867..3904)
                     /locus_tag="IV236_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8771777.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4225..5208)
                     /locus_tag="IV236_001584"
     CDS             complement(4225..5208)
                     /locus_tag="IV236_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8771778.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5623..6393)
                     /locus_tag="IV236_001585"
     CDS             complement(5623..6393)
                     /locus_tag="IV236_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771779.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6386..7321)
                     /locus_tag="IV236_001586"
     CDS             complement(6386..7321)
                     /locus_tag="IV236_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771780.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7637..9286)
                     /locus_tag="IV236_001587"
     CDS             complement(7637..9286)
                     /locus_tag="IV236_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8771781.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9403..12006)
                     /locus_tag="IV236_001588"
     CDS             complement(9403..12006)
                     /locus_tag="IV236_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8771782.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12018..13067)
                     /locus_tag="IV236_001589"
     CDS             complement(12018..13067)
                     /locus_tag="IV236_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8771783.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13093..13407)
                     /locus_tag="IV236_001590"
     CDS             complement(13093..13407)
                     /locus_tag="IV236_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8771784.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13423..>13813)
                     /locus_tag="IV236_001591"
     CDS             complement(13423..>13813)
                     /locus_tag="IV236_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8771785.1"
                     /translation="KKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGKNIA
                     IPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILAELS
                     GRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4063 a         2875 c         2372 g         4503 t
ORIGIN      
        1 gtgttatcat taagtggaaa caggaggtaa aaaaattatg acaactatga aaaagctaac
       61 ttttccagat gatttttggt ggggttccgc ttggtcagct gagcaagcag aaggaaaagg
      121 agaaacagga aaagcagaga ctatctggga tcgctggttt aaagaacaac cttaccggtt
      181 ttatcaacga atcgattcct ctattacaac cgatcacatc catcaatata aagaagatat
      241 agaactaatg aaaaaaactg ggcataactc ctttcgcgtt tctatttctt gggcacgaat
      301 gtttccaaat ggcggtaccg gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt
      361 attttccgaa atgaataata acggaatcaa agtatttgcc aacttatatc attttgacat
      421 gcctgctact cttcaagata aaggaggatg ggaatcacgt gaagtagttg atgcttatgt
      481 tcattacgct gaaacttgtt ttaaagaatt tggtgactta gtttgtcatt ggtttacctt
      541 caatgaacca cttgggccta ttttaggtac ttatcttgaa gattttcatt atccaaacct
      601 cattgacttt aaacgcggag cgcaagcagc attcttcact atacttgcac acgcaaaagc
      661 aatacaagta tttaaagaac tagctcttcc tagtaaaata ggcgttattc taaatttgag
      721 tcctactttt cctcgtagtc aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt
      781 attttatacg cggagctttt tagatcctat ggttaaaggg aatttttcta aaaagcttat
      841 cctgttatta caagaatata atcagcttcc tgaagattat acgaaagaag atcttgtatg
      901 tatctacgaa aataccgctc aaattcttgg actaaactat tacgagccac gtcgcgtcaa
      961 atctcgttct tctatggtgg atacatctag tccattttta ccagagtggt ttttcgaacc
     1021 acatatcatg cctggacgac gaatgaacga ataccgggga tgggaaattt acgaaaaagg
     1081 aatttatgat ttgtgcatgg atatcaagaa ccattatgga aacatcgaag catttatctc
     1141 ggagaacgga atgggcgttg cggatgaaga acgtttcatg gatgatacag ggcaaataat
     1201 tgatgattat cggattgagt atatcaaaga tcatcttgct tacttatgga aggcaataaa
     1261 tgaaggatgt aacattaagg ggtatcatct ttggacattt attgattgct ggtcatggat
     1321 caacgcctat aaaaaccgat atgggctggt ttcactcgac cttactactc aaaaaagaac
     1381 aattaaaaaa agtggcgaat tttacaagca attaagtgat acaaatggat ttagatacga
     1441 tgtagacttg ttagttgaat aatattctaa aattccactt gaagtgaaca cattagaaaa
     1501 caagaataat gagaaatacc aataacaaat actttttctt catacttcat ttatacttgt
     1561 cagtataaga gactgtgaca aatgtagtga tcctcagaag ttaggtctgg aaatctaact
     1621 tgagaagatt tttttaaact acgtccagtc ttttttattt gtttaaaatt gaagttgaca
     1681 agtcttcatt aaaaatttag aatattcaca agtttgacga caattatgaa aatttatgtc
     1741 gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg atcaaagaca
     1801 cgttacctac cgtttttggt tatatcggta ttggacttgc atttggtatc gttgggaaag
     1861 ctgccgggtt tcatccgctg gtcgtcacgt tgatgtccct gctgatctat gctggttctg
     1921 cccaatttat cacggtcagc atgcttgcta gccatagtcc actgctctcc atcgttctct
     1981 cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct ccttatttca
     2041 agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat gaaagtttcg
     2101 cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt tggatgaata
     2161 cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc gcacttttag
     2221 ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc gctatgttta
     2281 tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga ttacagttga
     2341 tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc ccaagtaact
     2401 tagtgatttt agttgtaaca ctgattgggt gcggattggg ggtgtggatc aaacatgcct
     2461 tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg gctttctagg
     2521 atcctaccat ttgtcttact gaaaaaattc gatttgccgc aaccgttgct tgagtattta
     2581 agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt tacccaaaat
     2641 atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc tacactttta
     2701 gctgctattc tttcaaaaag cttattggtg atcgtcttgg taggaattct ttccctttcg
     2761 ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg tcatgcgctc
     2821 ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg
     2881 gaccactaac aacgactttt cctaatatat gtcctgtttc tacgtctttg gttgcttgtt
     2941 tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg cggataatcc
     3001 cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt
     3061 tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg tttttccaaa
     3121 cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca aatggtcgga
     3181 caaggtgttt gatttgatca aagtagcggg taatatcgaa taatacagcg acatactcta
     3241 cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa tcgattggat
     3301 agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc gcgtagactt
     3361 ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgccccca gctccgttga
     3421 tcactaagat ttttttcccg cggttggcat tttcttctgg agtcagacca aatttttcaa
     3481 ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcggcttct tcatctgaaa
     3541 gattttttgg tgcaagagcg acaatacgtt cgtcaaccag ttggtattct tgattgcttc
     3601 ctgctcttgt cgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt
     3661 catcacctac agcaacaact ttcccgactc catcaaaccc taagatacgt aaagcatttc
     3721 ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact gcgttgactt
     3781 ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca ataaagcttt
     3841 ttggatcatc gatcgacaac ccttcataaa aaccgactac cttcattaat tccttttctg
     3901 acatacgaat tcctccttag cttcatcaag ttggagcatc catacagttt tcatttcaat
     3961 atatatagta tgctatttgt tccactttta ttcatcataa ttattctctt acttaaagta
     4021 aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct
     4081 tttaaacggt gttcaatcaa ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag
     4141 gagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacgacctct
     4201 aattcacggt gttcaagcaa gaaatcattg ttcactgaaa agcgttggat gttctttttc
     4261 aaaggtgtct aagatctctc gtacttcatc agtagatttc gtattcatca actgaactcg
     4321 caagtcattt gctcctggaa atcctttgat atagatttta aagaaacgat gtagtcctgt
     4381 gatggaacgc gggatgattt ctgaataatg atcttgcaga tctagttgca gacgaagtag
     4441 tccgatcagt tctttttctg tatgttccct cggttctttt tcaaatgcat aaggattctt
     4501 gaaaatcccc cggccaatca tgatgccatc tacgccatat ttttcggcca attcttctcc
     4561 tgtctggcga tctgggatat ctccgttgat cgtgatcagt gtttgcggag cgatttggtc
     4621 acgtaaagca atgattttag ggatgacttc ccaatgtgca tctactttac tcatctcttt
     4681 acgcgtacgc aaatggacag aaagattggc tatatcttga tgaagtaggt gactgatcca
     4741 ttcttccatc tcagacattt ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc
     4801 gccagcttta gccgcttcaa tcaattgtgc cgctacttct gggcgaagga tcaagccact
     4861 gccttttcca cgttctgcta cattagggac tgggcagccc atgttgatat cgattccttg
     4921 gaagcccatt tctgccatag caatgctcat ttctctaaaa aattctggct tgtctcccca
     4981 aatatgggca accatcggct gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc
     5041 ttttccatct ggatgacaga aactgtctga gtttgtaaac tcagtaaaga agacatccgg
     5101 tgctgctgca tgctttacga catggcgaaa tacaacatct gttacatctt ccattggtgc
     5161 taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta gtagtcattt atttgttact
     5221 ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca ttatactagg
     5281 aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt tctgattgag caaaaaaaga
     5341 ctgtgacaaa aatcttatca caattccttt tccggaataa atggtgttca aaagcttgca
     5401 tctgcggtaa taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
     5461 ttattacttg atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc
     5521 tgaccctcgg tattagctca acaaacgaca aaatatgagg aactattttc ccgtttgttt
     5581 ctctaatact caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct
     5641 tcctttattg atcaaatacc aagtaacggc gtacaaaaca acgatgaata gcacaagtac
     5701 gatcatcgat gttgctactg gcacatcgat tgttccaagg aatccgtaac ggaaaccgga
     5761 gatcatataa acgatcggat tgactttcga aacagcttgc caaaatggcg gcaacataga
     5821 gatggcatag aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat
     5881 cgatacatcg tcataagact gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa
     5941 gattgccgtc atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac
     6001 aaagaacaag gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccactccgcc
     6061 aatcacaaag ccccaaatga tcacatgcgt aggaacaggc gctaccaaga tttcttcgat
     6121 gtttttctgg aatttttgag agaagaagga cgaagaaacg ttggcatacg aactagtgat
     6181 agcagacatc ataatcaaac caggcacgat gaattccata tatgaaaaac cacccatgtc
     6241 accgatccga ccaccgatca tttttccgaa aataacaaag tacaaggatg tcgtgatcac
     6301 aggcggaacc agtgtttgga cccagatacg caaatagcgg ttcgtttcct tgatagctaa
     6361 actttttaat gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt
     6421 ctgtgatttt caaaaacagt tcttctaaac gattcgactt attcctcata gaaagaactt
     6481 tgattccttg tcttgtcagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt
     6541 cgacagcgat tgtctgttca tcttcaaatg tacaagcgta tccttcaatc acaggtttgg
     6601 tctcatatgg cgctaaatca aagataaatg tttcaaattg cagtttggat agaagctctt
     6661 tcatactagt attctcgatc agttcacctg attggatgat cccgatgttg cgacaaagca
     6721 tttcagcttc ttctaaataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt
     6781 ctcggagaaa ctcccacatc tcgcgtctca attcaatatc gactcctgct gttggttcat
     6841 ccaagatcag caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca
     6901 ttcccccgga aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca
     6961 aatatttttc gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat
     7021 tcacgacgat ttgctggacc gtttcaaagg gattgaaatt gaactcttgc gggactaatc
     7081 caatctgttg ctttgctcgg actagatccg tatccagatc ataaccgaag acattcactt
     7141 ttccagatgt tttattgact aaagaagtaa tgattccgat cgttgtagac ttcccagctc
     7201 catttggtcc taatagcgca tagaaatctc cttctttcac ggaaagatcg attcctcgca
     7261 atgcttcgac cccacttcca taaacctttt tcaaattatt tatttcaagc gcatttgcca
     7321 tttatttcct atctccttat agacatgatt ctttattgta tcatggaata cagcagatga
     7381 aaaaacaact tggttccagc gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga
     7441 aagacatcaa tcggacgaag tcagacaacc ttttcagaaa aaacaaatcg aagataaagg
     7501 attactattc atatgtgcaa caaaaggagc gtgagacaaa agtaaagaac acttttgtct
     7561 cacgctctaa acacgtataa acggcggaag caaaagccaa gatttttgat ccaataaata
     7621 ctttcgatca cttcgcctat cccaatacat gccactgccg cttttcatca aataacgcgg
     7681 cttccgtttt tgcggtttct actaaagtct ctgggtaatc caaaactttc agcaaggcaa
     7741 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
     7801 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
     7861 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
     7921 ctgaagcgga agaagcaatc cgttcgatcg tatttgtccc ttttaaaatc tcatctacga
     7981 agcagtagca gcgttctttg gaagccactt ggtccaacaa acgtttgatc gatttgattt
     8041 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
     8101 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
     8161 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
     8221 ctaatgtatt cttctgccag ttgaccggat tcactaccgc ttcttttaac aatggatgat
     8281 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atgggcatat
     8341 aagtacggta attcaatatt gcagcggcta cttctaattc tcccagcaat tcccacagct
     8401 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
     8461 gcagcatgaa catgatgttg acatattcaa aaatcaactc tgcttcactc ccactcttca
     8521 tacgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
     8581 cttcttgaag tggtgtcggc agttttgcta gctgattggc ggctgaaatc gttgaaacca
     8641 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
     8701 tattgaatac taacccaccg attgccaaat agattcctag tacttgtcct aaaagaagca
     8761 aacccaaacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
     8821 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
     8881 gagcgaattg atattggatt ttttctctca gttgaggatt ttcctgataa aaagcaatca
     8941 gtttttctaa ctgctgatct tcaccaaaat caaagctgcg taaacgttga tacaacgctt
     9001 ctgaacctac actagaatag gtactgttga ttccttcgaa tacttcgaac atatccaggt
     9061 cataccaagt aagatcatcg atttcgctat cccatgaacg gaacttcttc tccgtgagcc
     9121 atgcttcctt caagctttct tctttatcaa atctcggttg ataaggaatt ttttcccatt
     9181 tgctacgaac tgtttccttt aatttcaaac gtgaaaaaat gtctaaagca actaataaaa
     9241 taacagctgc aataataatt aaaacgataa attctctttg acccatatcc ctctccgttc
     9301 ctttatttgt tagctttagt atagcactgt tgcgctattt gaaaagaggg taaaaaaaca
     9361 cctttcaaaa taaaaggtgt ttttttcttg tatattacat atttatctct catttatcaa
     9421 aattgacaaa taatcatagg gaggaattgt tgttaattct tgtttattct ccagtgcatt
     9481 gacaactgtc ccaagggaaa ggatagcctc aggtactatt ttttcttgtt ctgttggatt
     9541 agctaagcga agaacaaatc cttgttcatg aaaataagaa ggataaatcg atgatacaat
     9601 taagtcttca ggtaattcaa tcaatgaaaa tttacgagga acatcaagtt cattttgtaa
     9661 tggccaaatc ttgttatcta acctgtgaat aaatacattc agtttctgct tttgataaga
     9721 aatagattgt tctaaccgtt tttctgcctg ttttgccact ttgaactcat taaatcttcc
     9781 ttcttctaca ttaaatccaa aagtgacttt atactcacct atctcttgag ctagcggtgt
     9841 ttccatcatg acatgccctt catttgtcgt atctcctgaa gctcgcccag gtcgccaagc
     9901 aagattaggt tttccaagtt cccctgttgt tgaaaataag gttatgatta attgattttc
     9961 tatacgttca tactctttta aaccatctgc ataaaacgta aaataatgat cattttctgc
    10021 cacagagaca ctcttatcga atatttctaa attaacaggt ttctctacca tagtttctga
    10081 ccaattcgga ggaatgacat caggggtatt ttcaataaat ccgttttgaa tttgtgcaat
    10141 tgatttttca gatttatcta aaacattaat tgctaagcgc atacgatgcg agtaaacagt
    10201 attatgaatt ttgagtgtgc cttcaacgat ttgactttct cccagactga acgatatttc
    10261 ataagatatt tcttccattt cgggtttttc agataaacga tcttttaaat ctttaggaag
    10321 ttgagctttt ccgtaaacaa ctaatgtttc ttgtaaagaa tctttatata ctttagctgt
    10381 ttctaatttt agagatagtt ctgtatctcc ttctaatggt gaataatcat acgtatcgcc
    10441 atcatttgct gaatcgatca aatgaacgaa atcaagatat tgtctatctc caactatcaa
    10501 attcaattgc ccatctttaa aagaaagttt atatttattg ttacttatct cagttttgtc
    10561 tatctcgatt gctgtttcta atttttcatt atttttctca aattcaacaa cagtgtatcc
    10621 tagtgctggc acatttacat ttatttttat gtcaagttca taataagctg gttcatcaaa
    10681 atattcgaaa cctgtagcaa tcatccgata agcatttttc cgttctggaa aataaacttc
    10741 tttttcaatg atattatagg gactaccttt gaagtgtatt ttctttgaat cagctactac
    10801 atgcacgatt ttcgatccac tataattctc agtcgttgtg ttgaaaacaa ggacttcatt
    10861 cgattttagc tttaatgcat cagccatccg ttttacaata aggttttcga ttccgtcagc
    10921 tatctcatta gcttctttga ttcgatggaa aatatcttcc gcaacattat ctgaaacaca
    10981 gccaccaata ctatcatgag cctggttttg caaaatcttt ttccataacc gacgaagtag
    11041 gcctttactg atatggatac catttctttc tgcaatcagc atgagcggct caactcgttt
    11101 tagaattttt tgttccaatt taaaattttc tattttcatt tttgttctta ctgagccaat
    11161 agaacgatga acacgagagt aaaccggctg acgaagttca cctttatatt caggtaattc
    11221 ttttttgttt ctgataactt ctacataaga aggataatct gaaataatat tgtggaattt
    11281 actgtattta ttcaacttct ctacgacttg atcaaaattt aaaatcattg acttttgatc
    11341 tacacccgaa ggtactaaaa tatctttgtt ttttccttgc atatgaagca tttcaactgc
    11401 ttcatccaac ttttcttttg caaattcagg aatatcttta attgcgtcta aggtcatcat
    11461 tccagtcata taaccaaatg gaaaattcat ggcataaaca aatttatctc ccaatccttt
    11521 ccagataaaa taaggagatg gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa
    11581 attatcgatt tctacttgat gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc
    11641 aggcaaatac cctaccatca ttggatctcc atatttaagg atcgtatcac tgattccaat
    11701 cattaagttt cttaaaattg attcagcatc cactaataag gcatctgttt gtgcatacca
    11761 cggtcccacg aatagttgtc ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg
    11821 attgatctcc aaatactcat cgacgattga agtttgtcca tctaaacaaa agtttacttt
    11881 agggttcctt tccaattcat ctagaacttc agtaaagacc tgatcagaca atacgatggc
    11941 atcttcattt gtaaaatacc attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt
    12001 taccatatca gattctccta ctttccttct tctactgaat ttacttgttg ttctttttta
    12061 tacttgatgg attttagtgc attgaccatt aacgctgtaa ccactccccc aataacaatt
    12121 gatactaaat ataaaggtat attcgtaaca aaaggaattg ccataaaggt tgaaagcatt
    12181 gtttcttgag taacaccaaa aaatccattt agaccacatg ctactgcagt gcccaccacg
    12241 atgcttggta ttactcgtaa aggatctttc acggcaaacg ggatcgtccc ttcagtaatc
    12301 cccacaaggc ccataatcat agctgtcttt gcattatctc gttccgattc atcataaagt
    12361 tttttgttta aaattgttgc taaaaacatt ccaaatggcg gaatagcaat tcctaataac
    12421 atggctgtac taggtccaaa gcttccgctt gaaaaagaag caaccccaaa agcatatgcg
    12481 attttattga taggtcctcc catatcaaac gccatcattc ctcctaaaat agccgacatc
    12541 aaagcagcat ttgctcctga catgccttct agccaattag tcagtcctgt tgtcattgct
    12601 tgaataggcg tagctagtac ataccataat atgattccaa caactaatgt agtaacaaaa
    12661 ggaatcagca taattgattt tagactaata acttgcatcg gtacttttac ttttttcaat
    12721 gcattagcta aatagccaca agctaatcca gcaaccattc ctccaataaa tcccatacca
    12781 agattttgac acatcatccc agtgataaaa gctggtgcta gaccgggacg gtcagcaatt
    12841 gaatatgcga tataggctga aatcatcggt acgataaact gtaatcccgt ctgaccaatc
    12901 attcgcagga ctccccagaa tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg
    12961 actgcaatag aagttagtaa ccctgctgct acgacaagcg ggatcatata ggaaacccca
    13021 ctttggaaat gatttagaat ttctcttcct tgtttcttga attccattag taactccccc
    13081 acgatatatt ttttatttgt taattaatgc aattgcttca tcaatcgtct cttcaccatc
    13141 ttttactacc tctgaggtcc ctactgttaa aatcggttta ccgtcaaagc gacctcgttc
    13201 tgaaacattt gtatctactg caaatatgac aacatctgcc tcggaaacat ccgaaattga
    13261 aagtttgttt tctatcccca tagccccctg tgtttctact ttaatatcta accctttctt
    13321 cttcgcaaac ttttctaaat tagcttttgc catataagta tgggcaatcc ctgcgataca
    13381 tgctgttact gctactattt ttgtcatatg aaattcctcc ttttaaaatg atagagcttt
    13441 taccaattct tttttatttc tcgaatcttt tattctttta acaaactcgt catccatcaa
    13501 tcttccagat aattctgcta aaatacgtaa atgattagta cctttatcag tgtcttttat
    13561 tgctaacaaa aagacgagat caactggttg atcatctaat gcgccccatt caacagaccg
    13621 ctttaacttg gctacagcca ctgttgattc aattactgct aggcttttgc catgaggaat
    13681 ggcgatattt ttcccaattc ctgtggtcat atggttttct ctgtcaaata cgtctttttg
    13741 aaattgtttg tggtcagaca caaacccatt agtctctaac aatttcgata atttttcgac
    13801 tacacttttt tta
//
LOCUS       DACDID010000069        13731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDID010000069 DACDID010000000
VERSION     DACDID010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.069"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IV236_001592"
     CDS             1..1452
                     /locus_tag="IV236_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8771786.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IV236_001593"
     CDS             1801..2433
                     /locus_tag="IV236_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8771787.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="IV236_001594"
     CDS             3021..4460
                     /locus_tag="IV236_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8771788.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="IV236_001595"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="IV236_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="IV236_001596"
     CDS             5222..5437
                     /locus_tag="IV236_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771789.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="IV236_001597"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="IV236_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6097..7008)
                     /locus_tag="IV236_001598"
     CDS             complement(6097..7008)
                     /locus_tag="IV236_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8771790.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="IV236_001599"
     CDS             complement(7027..8031)
                     /locus_tag="IV236_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8771791.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="IV236_001600"
     CDS             complement(8028..10151)
                     /locus_tag="IV236_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8771792.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="IV236_001601"
     CDS             complement(10156..12603)
                     /locus_tag="IV236_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8771793.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="IV236_001602"
     CDS             complement(12590..12979)
                     /locus_tag="IV236_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8771794.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="IV236_001603"
     CDS             complement(13039..13542)
                     /locus_tag="IV236_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8771795.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..>13731)
                     /locus_tag="IV236_001604"
     CDS             complement(13555..>13731)
                     /locus_tag="IV236_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771796.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DACDID010000070        13716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDID010000070 DACDID010000000
VERSION     DACDID010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.070"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            206..544
                     /locus_tag="IV236_001605"
     CDS             206..544
                     /locus_tag="IV236_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771797.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="IV236_001606"
     CDS             complement(749..1324)
                     /locus_tag="IV236_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8771798.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="IV236_001607"
     CDS             complement(2056..2637)
                     /locus_tag="IV236_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8771799.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="IV236_001608"
     CDS             complement(2820..3446)
                     /locus_tag="IV236_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8771800.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="IV236_001609"
     CDS             complement(3468..4799)
                     /locus_tag="IV236_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8771801.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="IV236_001610"
     CDS             complement(4954..5355)
                     /locus_tag="IV236_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8771802.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="IV236_001611"
     CDS             complement(5441..5647)
                     /locus_tag="IV236_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771803.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="IV236_001612"
     CDS             complement(5742..7244)
                     /locus_tag="IV236_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8771804.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IV236_001613"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IV236_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8771805.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="IV236_001614"
     CDS             complement(7815..8432)
                     /locus_tag="IV236_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771806.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="IV236_001615"
     CDS             8653..10026
                     /locus_tag="IV236_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8771807.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="IV236_001616"
     CDS             10101..11213
                     /locus_tag="IV236_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8771808.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="IV236_001617"
     CDS             complement(11285..12922)
                     /locus_tag="IV236_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8771809.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="IV236_001618"
     CDS             complement(13090..13698)
                     /locus_tag="IV236_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8771810.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3703 a         2884 c         2461 g         4668 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttc
//
LOCUS       DACDID010000071        13662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDID010000071 DACDID010000000
VERSION     DACDID010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.071"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..2606
                     /locus_tag="IV236_001619"
     CDS             135..2606
                     /locus_tag="IV236_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8771811.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="IV236_001620"
     CDS             complement(2709..4445)
                     /locus_tag="IV236_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8771812.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="IV236_001621"
     CDS             complement(4442..5146)
                     /locus_tag="IV236_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771813.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="IV236_001622"
     CDS             5277..5780
                     /locus_tag="IV236_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8771814.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="IV236_001623"
     CDS             5740..6078
                     /locus_tag="IV236_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8771815.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="IV236_001624"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="IV236_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8771816.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="IV236_001625"
     CDS             7629..8207
                     /locus_tag="IV236_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8771817.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="IV236_001626"
     CDS             8211..8732
                     /locus_tag="IV236_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8771818.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="IV236_001627"
     CDS             complement(8811..9365)
                     /locus_tag="IV236_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8771819.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="IV236_001628"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="IV236_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8771820.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="IV236_001629"
     CDS             9905..10156
                     /locus_tag="IV236_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8771821.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="IV236_001630"
     CDS             10281..11072
                     /locus_tag="IV236_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771822.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="IV236_001631"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="IV236_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8771823.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="IV236_001632"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="IV236_001632"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8771824.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="IV236_001633"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="IV236_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8771825.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..>13662)
                     /locus_tag="IV236_001634"
     CDS             complement(13129..>13662)
                     /locus_tag="IV236_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8771826.1"
                     /translation="YSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMYR
                     ERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTA
                     INTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEKIYL
                     HVTKGVKEKLKEDIEKL"
BASE COUNT         4485 a         2495 c         2805 g         3877 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa ta
//
LOCUS       DACDID010000072        13597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDID010000072 DACDID010000000
VERSION     DACDID010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.072"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(119..1066)
                     /locus_tag="IV236_001635"
     CDS             complement(119..1066)
                     /locus_tag="IV236_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8771827.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1256..1525
                     /gene="rpsN"
                     /locus_tag="IV236_001636"
     CDS             1256..1525
                     /gene="rpsN"
                     /locus_tag="IV236_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8771828.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1701..2063)
                     /locus_tag="IV236_001637"
     CDS             complement(1701..2063)
                     /locus_tag="IV236_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8771829.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2074..3195)
                     /locus_tag="IV236_001638"
     CDS             complement(2074..3195)
                     /locus_tag="IV236_001638"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8771830.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3211..3561)
                     /locus_tag="IV236_001639"
     CDS             complement(3211..3561)
                     /locus_tag="IV236_001639"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8771831.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3691..5202)
                     /locus_tag="IV236_001640"
     CDS             complement(3691..5202)
                     /locus_tag="IV236_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8771832.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5524..6876)
                     /locus_tag="IV236_001641"
     CDS             complement(5524..6876)
                     /locus_tag="IV236_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8771833.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7029..8105)
                     /locus_tag="IV236_001642"
     CDS             complement(7029..8105)
                     /locus_tag="IV236_001642"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8771834.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8301..10046
                     /locus_tag="IV236_001643"
     CDS             8301..10046
                     /locus_tag="IV236_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8771835.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10326..10763)
                     /locus_tag="IV236_001644"
     CDS             complement(10326..10763)
                     /locus_tag="IV236_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8771836.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10747..12120)
                     /locus_tag="IV236_001645"
     CDS             complement(10747..12120)
                     /locus_tag="IV236_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771837.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12442..12930)
                     /locus_tag="IV236_001646"
     CDS             complement(12442..12930)
                     /locus_tag="IV236_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771838.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12952..13554)
                     /locus_tag="IV236_001647"
     CDS             complement(12952..13554)
                     /locus_tag="IV236_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8771839.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3894 a         2947 c         2461 g         4295 t
ORIGIN      
        1 gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc taaaacattt
       61 tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc tgtatttctt
      121 atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact agctgatgtg
      181 cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca tgaaaggctc
      241 ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat tcctttcttg
      301 gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct gggaggtcat
      361 ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct cctttgatac
      421 ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga cctggatttg
      481 ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc tcatttttac
      541 tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc agacttgacc
      601 cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt tccgtcaaat
      661 ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct tcttcccaac
      721 tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct tctccgtaag
      781 tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg acatctgaac
      841 caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat ttcaatgttc
      901 gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca ctgaatgctt
      961 ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg atttcttcaa
     1021 aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc accatctccc
     1081 tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct tttcgttttg
     1141 gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa ttatttatct
     1201 aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt tgttgatggc
     1261 taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac gctatgctgc
     1321 tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac ttcctttaga
     1381 tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc gcggatacat
     1441 gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag gtttgattcc
     1501 aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg aggagggcgc
     1561 atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca gaaatgctgg
     1621 tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata tgtaaaggct
     1681 gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat acccacacta
     1741 attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca gaccttttct
     1801 ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga gtctctttct
     1861 attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt agccattttt
     1921 gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt attttgaata
     1981 accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag atcagcaaaa
     2041 taaatatctc ctctttttac cataggatcc tccttattct tgatattctc ttggtatccg
     2101 ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg ctatatcctg
     2161 catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc cgacaggaaa
     2221 ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg tttcacagta
     2281 gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat ctgcataacc
     2341 gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc catagccgat
     2401 tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct caagtgctgg
     2461 ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt acattgctac
     2521 tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg cactattgct
     2581 gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa agcgttcttt
     2641 ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg taaataaacc
     2701 ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta cttcttcagg
     2761 tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg aaagaggcgt
     2821 ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc gtgtacctgc
     2881 agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa ccatattcaa
     2941 gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat ctaaatttga
     3001 aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa tagctccatg
     3061 accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa gtcttgcaac
     3121 ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg ttgggcggtg
     3181 ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt gtgcaaaagc
     3241 tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg aacgcgtcac
     3301 aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa cttttccgat
     3361 cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc ctaaaaattc
     3421 tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg tcaagattcg
     3481 tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt cgactgcatc
     3541 gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa cactctcatt
     3601 ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa tgaaagtttc
     3661 tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga ttacaaatcc
     3721 gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat ttttgctata
     3781 accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac ggttgtttcc
     3841 gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg ggcgttctgg
     3901 tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta gcaaaagtgc
     3961 aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca ggtatttatc
     4021 tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta attgaccttt
     4081 gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg tcaagttttc
     4141 aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca taccgccttt
     4201 tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt gagggatatc
     4261 atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa cgtctgtcgc
     4321 tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc gtttttgttg
     4381 agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc cgcgtgccaa
     4441 ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg tttgaacatc
     4501 aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat agtattgatc
     4561 gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat ttttcatgaa
     4621 tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta atgtttgtct
     4681 ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca tattcaacat
     4741 ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg tgtggcggtt
     4801 aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat ctttcaagcc
     4861 acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta cttttttgtc
     4921 gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg ttggtgcgat
     4981 aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg gtaatccaaa
     5041 tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc cagctaatgc
     5101 tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc gttcgactga
     5161 tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc tcctatatgg
     5221 ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc aaagtgtatt
     5281 gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg tggcaccacc
     5341 gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg ttcagcttta
     5401 tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag tccaatttct
     5461 cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca agtttacaga
     5521 tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc tgttgctgcc
     5581 ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa tcaatttatt
     5641 cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca attcttcttt
     5701 tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag gatcgatatg
     5761 ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca tatctgctaa
     5821 cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata ccagtcccat
     5881 ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc ctgccgttag
     5941 ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta tctcaggtat
     6001 ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta cattataact
     6061 tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt gtgcttttcc
     6121 ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg tgactgtttg
     6181 tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa cgagtaatcc
     6241 gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt ctcgcgaccc
     6301 aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg gttgagccat
     6361 aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa cgatcttttc
     6421 tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt tgtaatttcc
     6481 ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat ctttcgtggt
     6541 ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt ccaagtaata
     6601 ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg gaaaattttt
     6661 tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg tgatcgcttg
     6721 ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac ttccttcggt
     6781 tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc attcgctgcc
     6841 cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta aaaaaggttg
     6901 tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca agtaaatttc
     6961 tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag cctcttcttt
     7021 atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg attcattcct
     7081 aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc ccataagagt
     7141 gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag gaataattca
     7201 tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa catcgtgtaa
     7261 gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc ggctggaatc
     7321 tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc tacgtctttt
     7381 acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc aacttcgatt
     7441 tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata ggctgttgct
     7501 aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact agaacccata
     7561 ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc aaatactttt
     7621 ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg cggcacacca
     7681 gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact ggtcaatacg
     7741 cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt tccatcttcc
     7801 ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat ttcgcccgga
     7861 ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca tgaaagatgc
     7921 aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca ttgtccttct
     7981 tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt taaaactgaa
     8041 aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag taaagtaatc
     8101 ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca tattttagca
     8161 caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc taaaagaacg
     8221 agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa aagttatgat
     8281 aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg gcagaaatca
     8341 ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa aatcccgaac
     8401 aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga cagatgcttt
     8461 ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca ttacaagata
     8521 gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa ctttctcttt
     8581 cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct tctatggggc
     8641 aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc caacacccta
     8701 acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc cctttgatca
     8761 aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa gtaaaacgtt
     8821 ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa ttctatcaaa
     8881 agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt tgttcgaaaa
     8941 aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg aacgccgaaa
     9001 acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa gaagttgcaa
     9061 agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc catgctgatc
     9121 ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca caagctagcc
     9181 gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga gcctccactt
     9241 ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt atgatggggc
     9301 atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg tatacgaaag
     9361 atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc tcgtttgatg
     9421 aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat tctcctattg
     9481 atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat caaaataatc
     9541 tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta gaaggggtga
     9601 tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt gctcaacatt
     9661 attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta gacttattgg
     9721 aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta gaaatgtgga
     9781 cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta ttgagcagga
     9841 tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg atcgtagggt
     9901 cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc ttattaggga
     9961 tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat gcattgatcc
    10021 aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc agcttcaaga
    10081 aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc ggcttatttt
    10141 tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg ttctgcaccc
    10201 agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc cctgttccct
    10261 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10321 aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc tttctccgta
    10381 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10441 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10501 cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10561 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10621 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10681 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10741 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10801 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10861 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10921 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10981 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg tatcagtttg
    11041 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11101 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11161 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11221 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11281 cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11341 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11401 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11461 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11521 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11581 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11641 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11701 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11761 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11821 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11881 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11941 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    12001 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12061 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12121 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12181 gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa gaagcataag
    12241 cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt ctccacaaaa
    12301 gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat tatcctgtgc
    12361 ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc gcataaattt
    12421 actttaacac agttcctatg tttatgcgag agcttttttc attttataat cgaccatttg
    12481 aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt catgcttata
    12541 aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc tttcctttgt
    12601 ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt gaccacgtac
    12661 tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt cataagcagt
    12721 gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt ctgtcagttc
    12781 tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt gtttttgacc
    12841 aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca gctcatccag
    12901 ttcattttct gttactttta ttttttccat acacccacgt ccttttttac attataaatg
    12961 accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca ccgaccacgg
    13021 tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca aaccagctac
    13081 tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta gtactttttt
    13141 aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt ttggcgataa
    13201 aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga ttgcttcccc
    13261 aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca tcagcatgcc
    13321 cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca cttgcaacag
    13381 aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca aagacgtgct
    13441 cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa aaaacagacc
    13501 aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag acatctatat
    13561 tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DACDID010000073        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDID010000073 DACDID010000000
VERSION     DACDID010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.073"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV236_001648"
     CDS             complement(14..706)
                     /locus_tag="IV236_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8771840.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV236_001649"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV236_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV236_001650"
     CDS             complement(1143..1712)
                     /locus_tag="IV236_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8771841.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV236_001651"
     CDS             complement(1786..2109)
                     /locus_tag="IV236_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771842.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV236_001652"
     CDS             complement(2263..3075)
                     /locus_tag="IV236_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771843.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV236_001653"
     CDS             3429..4277
                     /locus_tag="IV236_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8771844.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV236_001654"
     CDS             4422..5363
                     /locus_tag="IV236_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8771845.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV236_001655"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV236_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV236_001656"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV236_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8771846.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IV236_001657"
     CDS             complement(9827..12013)
                     /locus_tag="IV236_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8771847.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV236_001658"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV236_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8771848.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IV236_001659"
     CDS             complement(12235..12675)
                     /locus_tag="IV236_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8771849.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IV236_001660"
     CDS             complement(12749..13288)
                     /locus_tag="IV236_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8771850.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDID010000074        13369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDID010000074 DACDID010000000
VERSION     DACDID010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.074"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..1439
                     /locus_tag="IV236_001661"
     CDS             12..1439
                     /locus_tag="IV236_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8771851.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1531..1896)
                     /locus_tag="IV236_001662"
     CDS             complement(1531..1896)
                     /locus_tag="IV236_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8771852.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1893..2258)
                     /locus_tag="IV236_001663"
     CDS             complement(1893..2258)
                     /locus_tag="IV236_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8771853.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2450..2698)
                     /locus_tag="IV236_001664"
     CDS             complement(2450..2698)
                     /locus_tag="IV236_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771854.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2697..2825
                     /locus_tag="IV236_001665"
     CDS             2697..2825
                     /locus_tag="IV236_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8771855.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2882..4576
                     /locus_tag="IV236_001666"
     CDS             2882..4576
                     /locus_tag="IV236_001666"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8771856.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4676..4849
                     /locus_tag="IV236_001667"
     CDS             4676..4849
                     /locus_tag="IV236_001667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771857.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5227..6360
                     /locus_tag="IV236_001668"
     CDS             5227..6360
                     /locus_tag="IV236_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8771858.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6391..6711
                     /locus_tag="IV236_001669"
     CDS             6391..6711
                     /locus_tag="IV236_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771859.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6740..7204)
                     /locus_tag="IV236_001670"
     CDS             complement(6740..7204)
                     /locus_tag="IV236_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8771860.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7336..8589)
                     /locus_tag="IV236_001671"
     CDS             complement(7336..8589)
                     /locus_tag="IV236_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8771861.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8618..8935)
                     /locus_tag="IV236_001672"
     CDS             complement(8618..8935)
                     /locus_tag="IV236_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8771862.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8961..9281)
                     /locus_tag="IV236_001673"
     CDS             complement(8961..9281)
                     /locus_tag="IV236_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8771863.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9745..11160)
                     /locus_tag="IV236_001674"
     CDS             complement(9745..11160)
                     /locus_tag="IV236_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8771864.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11177..12493)
                     /locus_tag="IV236_001675"
     CDS             complement(11177..12493)
                     /locus_tag="IV236_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8771865.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12643..13341)
                     /locus_tag="IV236_001676"
     CDS             complement(12643..13341)
                     /locus_tag="IV236_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8771866.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4229 a         2726 c         2308 g         4106 t
ORIGIN      
        1 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
       61 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      121 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      181 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      241 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      301 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      361 gcaaagctta ttcattaaaa aaacaagtat tagcttactt taatgactgc gggatcgaaa
      421 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      481 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      541 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      601 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      661 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      721 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      781 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      841 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      901 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
      961 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1021 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1081 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1141 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1201 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1261 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1321 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1381 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1441 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1501 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta ggggtccgat
     1561 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1621 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1681 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgcttactcg
     1741 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1801 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1861 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1921 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1981 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2041 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2101 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2161 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2221 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2281 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2341 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2401 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2461 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2521 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2581 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2641 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2701 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2761 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2821 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2881 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2941 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3001 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3061 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3121 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3181 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3241 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3301 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3361 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3421 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3481 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3541 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3601 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3661 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3721 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3781 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3841 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3901 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3961 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4021 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4081 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4141 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4201 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4261 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4321 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4381 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4441 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4501 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4561 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4621 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4681 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4741 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4801 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4861 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4921 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4981 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5041 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5101 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5161 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5221 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5281 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5341 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5401 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5461 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5521 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5581 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5641 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5701 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5761 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5821 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5881 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5941 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6001 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6061 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6121 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6181 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6241 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6301 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6361 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6421 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6481 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6541 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6601 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6661 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6721 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6781 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6841 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6901 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     6961 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7021 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7081 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7141 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7201 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7261 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7321 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7381 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7441 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7501 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7561 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7621 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7681 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7741 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7801 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7861 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7921 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     7981 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8041 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8101 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8161 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8221 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8281 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8341 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8401 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8461 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8521 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8581 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8641 gattaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8701 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8761 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8821 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8881 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     8941 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9001 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9061 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9121 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9181 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9241 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9301 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9361 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9421 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9481 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9541 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9601 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9661 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9721 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9781 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9841 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9901 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
     9961 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10021 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10081 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10141 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10201 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10261 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10321 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10381 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10441 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10501 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10561 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10621 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10681 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10741 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10801 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10861 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10921 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    10981 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11041 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11101 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11161 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11221 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11281 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11341 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11401 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11461 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11521 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11581 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11641 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11701 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11761 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11821 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11881 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    11941 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12001 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12061 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12121 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12181 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12241 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12301 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12361 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12421 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12481 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12541 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12601 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12661 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12721 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12781 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12841 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12901 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    12961 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13021 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13081 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13141 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13201 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13261 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13321 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgta
//
LOCUS       DACDID010000075        13314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDID010000075 DACDID010000000
VERSION     DACDID010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.075"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..132
                     /locus_tag="IV236_001677"
     CDS             1..132
                     /locus_tag="IV236_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8771867.1"
                     /translation="MATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(194..2005)
                     /locus_tag="IV236_001678"
     CDS             complement(194..2005)
                     /locus_tag="IV236_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8771868.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2002..2367)
                     /locus_tag="IV236_001679"
     CDS             complement(2002..2367)
                     /locus_tag="IV236_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8771869.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2530..2925)
                     /locus_tag="IV236_001680"
     CDS             complement(2530..2925)
                     /locus_tag="IV236_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8771870.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2943..3905)
                     /locus_tag="IV236_001681"
     CDS             complement(2943..3905)
                     /locus_tag="IV236_001681"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8771871.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3905..4117)
                     /locus_tag="IV236_001682"
     CDS             complement(3905..4117)
                     /locus_tag="IV236_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8771872.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4138..4836)
                     /locus_tag="IV236_001683"
     CDS             complement(4138..4836)
                     /locus_tag="IV236_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8771873.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4856..5398)
                     /locus_tag="IV236_001684"
     CDS             complement(4856..5398)
                     /locus_tag="IV236_001684"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8771874.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5530..6534)
                     /locus_tag="IV236_001685"
     CDS             complement(5530..6534)
                     /locus_tag="IV236_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8771875.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6531..7520)
                     /locus_tag="IV236_001686"
     CDS             complement(6531..7520)
                     /locus_tag="IV236_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8771876.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7517..8323)
                     /locus_tag="IV236_001687"
     CDS             complement(7517..8323)
                     /locus_tag="IV236_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771877.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8489..9445
                     /locus_tag="IV236_001688"
     CDS             8489..9445
                     /locus_tag="IV236_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8771878.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9522..10040)
                     /locus_tag="IV236_001689"
     CDS             complement(9522..10040)
                     /locus_tag="IV236_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8771879.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10128..10277)
                     /gene="rpmG"
                     /locus_tag="IV236_001690"
     CDS             complement(10128..10277)
                     /gene="rpmG"
                     /locus_tag="IV236_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8771880.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10505..10951
                     /locus_tag="IV236_001691"
     CDS             10505..10951
                     /locus_tag="IV236_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8771881.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11146..13041)
                     /gene="asnB"
                     /locus_tag="IV236_001692"
     CDS             complement(11146..13041)
                     /gene="asnB"
                     /locus_tag="IV236_001692"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8771882.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4142 a         2830 c         2269 g         4073 t
ORIGIN      
        1 ctggcaacag ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat
       61 ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc
      121 gtttctcgtt aataattatt taaaaatacg catgaagact ttctaaaaaa cagataagtc
      181 atcatgcgta tttttatttg tcagtcaata taagttgtgc tattttatct gcctgtctca
      241 gattccatgg atctgtataa ggtctagcta ctgctgcagt tagccaatcc acaccttcaa
      301 caggtattcc atagcaataa aagtcagact ggatttcccc tgaagcatca atcacgcgat
      361 tcgtacctcg ctcaacatct atagcttctg aatgataact tcctgtatta tctgaaaatg
      421 aaaaagggcg aacaagtttg ttttccttca aattttttat gacaggatta cttgaacgag
      481 aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt tctttatctt
      541 gtagagaaat aacatagcgg ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat
      601 gaaaaatacc cgcatctatc agtgctgcta attctttcgt ccgaatgatt ggcggaccga
      661 tcgttaaaaa gctatttaga tgagtaaacc atccccatag tatttctttg tattccttag
      721 cagaaaaaac gtgccattca atagcctgcc ggattggatc acgccagtct tttaatgcat
      781 caaatgttga agcaaatgct cctgtgcaat tgcctttttc cgcttctttt atctgccctt
      841 caataaatat tcggctagct tctgaaaagg gctcattctg ataggcaaga ggagtttcta
      901 aatacgacca atcccatcta taagggatta gtgctggatg ctttccgaga cactcctctg
      961 aagagaggga gagaaaatca tgctcaaaat ttttctttgg aatcggcaag tgatattctt
     1021 cgatgatttt cttgtaataa aacaactcaa cgtctttttt cagatagtca aaaaatgttt
     1081 cgccagtcaa aaatcccgac tgatgccatt gctccaggtt ttcttttgtt agtaaccgtg
     1141 gccatgccat tgctccgcct tgcttttgat ttcttcctcg agggtaataa gggagccccc
     1201 ttcttgatcc actgtataca atcggttcct tgcctgatgg atgatagacc agttttccct
     1261 tttcgttctc gaagcgccct cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac
     1321 ttaagcctag acctcttaaa aagacatttt ctttaggcgg tatcttcgaa agatctacat
     1381 ctgcaggatt agaaggaagc tgatacagta aaacattttt ctttgcgtag tctacccaca
     1441 ctctttcttc ttctgtctca tcattttccc agtgaccgct tgctaaaaca agctgatcga
     1501 tagaatagct ttgctgatct gtctgtaaaa gataaccatc ttttcttttg cttacttttt
     1561 ttactaaaga ataaacaaac tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa
     1621 accagcgttg atagagtccg tataaacatc gagtagattg ctgatctttt tccagacgat
     1681 tgatttcttc tataattagt tgcttatttt tggttaccgt ctgttcgata taatctgtag
     1741 caggtccttt actccattga aacaagttgg gtccctgtgt taccaatcct ccactggaca
     1801 atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga attcattaat aactccggtg
     1861 gctgattcaa ccgccaaact cgacctccag gaccattggg atcgaaaaga aaaagttcaa
     1921 gagaatgtct gtcctgatta ttatggagaa tcctttctgc tacacttaat cctctaggtc
     1981 cggcaccaat gatccctatt ttcatttgca tgcctccatt tcgataaatt ttttcaataa
     2041 aacagcatgg ttctgatgtt cattttttgc accataaaca aaagtaatga ctggatgttc
     2101 tttcataagt tgactgagtt taaaaatagc ttgcttcttt tcttcttcga aaatcaattc
     2161 ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa aagttttgcg
     2221 tgcttcatta ctaggtgcaa tttctttcaa ccacagatct aactgcgctt tttcttttga
     2281 aattcctcgt ggccagaatc tatccacgag tattctatag ccatcctctt tttcagcatc
     2341 aaggtaaaca cgttttaatt tgatcatagt tgtttccttc tttctacctt ttcgcttagc
     2401 ttatatatgt agataatagc acaaaataaa aaaaatgaac ggcacaacta tccattgcgc
     2461 cgttcatgtg atcttggtca aaatctaatg attgattcgg tttttaaagc tgtcatacaa
     2521 tcatctgttt tatacttttt tacttttgat ttttccagac catccgtcat atccaccctt
     2581 aagtaaataa atctcttgat aacctgcttt acgtaattta tttgctgtac ggatgctcaa
     2641 acttttcttt tgatcgtaaa tatagacagg ctggtctttt cgaatagatc ctaatgaatc
     2701 tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata tgcccagcat caaacacatc
     2761 tttttcgcga acatcgatta cttgtgcttt tctcattcct tctcgaaatt cttcttctgt
     2821 caaagtcgtt gctgaacgtt tggccattac tcgtagatag acttcccaaa ggatgattgc
     2881 aagcacaatc aatagcaaaa tgatattaat tacccataaa accatgtaat gcctccgttt
     2941 ttttaagcaa atttcaaggc taatgaggca gcaccaatga caccagcttc attaccaagc
     3001 tctgctaatt tgatttttgt tgattctctt acttgcggga aagtaaattc tttaaaatat
     3061 ttctctacac ggctacgtaa aaattctcct gctgccgata ctcctccacc taacacgata
     3121 cttgacggat ttaatgtatt ccccaagttt ccgcaagcta aacctaagta gaagcatacg
     3181 cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa gatatcttta
     3241 cttgttactt cttctccgtt gtctagcatt gctttcagtt ttgaatcccc tgcgtattct
     3301 tctgccaaga aacgacctag gcgtaccaca cctgtagcgc tagatacagt ttctaaacat
     3361 ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg tccgatttct
     3421 cctccagctc cgctcacgcc gtgaatcaaa tttccgccag caacgatacc tccgccaaca
     3481 cctgttccta gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc tttccatcgt
     3541 tcacctaaag cagcaacatt cgcgtcatta tcgatagcga atttgattcc tgttcctgcc
     3601 tcgatcagtt ttttcacaaa ttgaacttct gtccagttta agttatacgc accaataacc
     3661 gttccagctt cgctgtccac gcttcctggg gtacccatgc cgattccgat aaagtcttcg
     3721 gctttcatat tatataaatt caaacgatga ttgattgatt caatgatttc aggaacgata
     3781 tgcttaccat catccaaaat attggtatcg atgctccatt tttgctgaat ctctccttct
     3841 ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat caatcccaat aatcttcttg
     3901 tccattacaa atttcctttc tttcggcttt cttcaattcg gtgctctctt tttaatatgc
     3961 gccacgctgt catgtatgta tcatgatcaa tcaaacggct atcatacaga ttacgcaatt
     4021 cgatagtcat taattcaatg tcatagatgc gtgcacccac atagataaag atgccaaatt
     4081 ttttgaacag ctgctgtaca tcatataagc tctccatctt taagcacttc cttcatatta
     4141 tacaagtaaa ccatattttt ttaaacccag taaaatacat attacaaaaa gaaatacaaa
     4201 aactagtgta gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt tcggtagacc
     4261 caaaacattc cctaacacta atccgccaat tagcccaccg acatgtcccc aaacatctac
     4321 agaggtatcc agaagaccaa aaacaaatgt caacacaata aataaaagaa aatgtctgac
     4381 tagctgctgg atcgctgggt tatgtttaaa atgaaaccct aagataaaca acgcaccgaa
     4441 caaaccgaat atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa
     4501 gcttgctgta tttcccatta tcccgctgaa catataaatc aagaaaaaac gccaatggcc
     4561 atagattgcc tcgatttgct gccccataaa atacagtatc agtgagttca ttacgaaatg
     4621 catcagtcca aaatggatga agattggtgt cactaaacgc caccattcat tgaagtgaac
     4681 aagataagga ccatacattc cggcataata agggatctca agtcgcggaa ataattccat
     4741 catgaggaag accgctgtct gtatccctaa aaaagcatag gtccataacg gtcgtcttaa
     4801 ccatgcacgc aattttattt gctgttgata attcatgaaa acctcccatt tcattttagt
     4861 caataaacaa ttgttggatc ttctgatcaa agctctccgg ttgccattgt tcattcaatt
     4921 gttcacgaaa aacaaggcta catgtttttt tatgaaaatt atttaaatac cggtcgtaat
     4981 aaccaccgcc aaaaccgatt cgataaccgg ctctattgta gataatacct ggtacaatga
     5041 ggagatcgat catctccggc aaatagagct tctctgattg tggctcaaga acaccaaaac
     5101 tcgaacgtac aagaacggac tgttcatcca tttcataaaa cgccatctga cgtttcggca
     5161 aagttttagg tacaactatt ttcttttgtt ctttttctgc tcgttggata accggctgtg
     5221 tatccagttc aaattcattc gataaagtcg tccctatcac ttttgctgac ttccagtact
     5281 cgctggcgaa tagctgttta tagattcgct gttctttttc ctttttcttg ccacttttgg
     5341 ccagattttt cagcttttcg attcctttcc ggcgtaatct tactttcaat tggtccaaaa
     5401 actcaccctt ccttctctca taaaatggta taggaaaaaa agaaaaatag caatcgaact
     5461 ttcaaataaa tgcataaata gaataaaaag aagacctaga aatgattggc cttccttttt
     5521 taatgaattt caactgttca gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata
     5581 tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact tccccacttg gtagaatcac
     5641 tctagcaacc atatctgcac taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa
     5701 ggtccagtta ttttctttac gtacgactag cttcgcagca tgaggagcta gtagtcctac
     5761 aaaagataaa ctacctgtca ccgctacact accgcttgcc aaagcaacag ctaacaacaa
     5821 aaggattttt tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa
     5881 tgatagcaat acaatcctct gtgcataaag tcctcctagc ggaattaaga taaccgccca
     5941 tggaagcaat gcccaaacat aaggccagct cgcaccccag attgatcccg ctaaccatgc
     6001 attcacaaat ccataatttt ccttagataa ttggatcgtg catagcaatg ttaatgcaga
     6061 aaggccggca ttaacggcaa taccggacaa taagattcga ttcataccca aaaagccatt
     6121 tcgatcatag gccgcaagat agacacagca agcagctaaa acaccaccta gaaaggctac
     6181 aactagcaat agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa
     6241 aaatcctgct ccggcattaa tgcccaatat actagaatct gccaattcat ttcttgttac
     6301 tccttgcaag atataaccag aaacgccaag acaagctcct gctaataaac tgatcagtaa
     6361 tcgaggaact cgaaattctc taacaatcag cgccaaagaa gatgattctt tccctatgaa
     6421 cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca atcattaaac tacctataga
     6481 caggatgaca agtacaaaaa gcaagaaata gaacgtccaa tatatttttt tcaccgttgt
     6541 tccctcctta cttgtatcag taaaaacgga acgccaatca atgcaatgac taatccaaaa
     6601 ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat ccgccaacat aaccaatcca
     6661 ccgccaacta aaaaagagac aggtagaaca taacgatagt ccgttccaac cattccgcga
     6721 acaactggcg gaaccaacag cccaataaat gaaacaggtc cgactaacga aacggcaatg
     6781 gctgctaaca ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg atgacttcca
     6841 agagctaatg catgtgtttc actcaaatga atcaaagata ttgcttttcc taatgaaaaa
     6901 gaaaccaaaa cagcaagtat atataaagga aacaatactg ctaattgttt ccatgtgata
     6961 ttggcagctc cgcctacaaa ccaaaaagca agatcctgct gcagattaaa gaattgggta
     7021 agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt
     7081 aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa agttacagca
     7141 aaagaacctg ccaatgacat tagaaacaca gaaaaatcag aagctttagg ccaaaaagca
     7201 aaactaaggg ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct
     7261 aaaggatttc ttgtcacccc ttgcatcaat gcaccgctca atgcaaaaca actccctaca
     7321 agaaatgctc ccgccacacg aggcaaacga atatttcgta tgatttggtg actttgtttt
     7381 gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt tttccacagt cgctgcgcca
     7441 tttgaaagag caacaaaaaa aataatcaaa agaacaaaac agaccaaaag caggagtcta
     7501 tttttcttca tagtcctcat aattgatcct cctgctcttt tggcaacaaa tcataggtta
     7561 gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt
     7621 ttttcataca ctcttttgtg atcacttctt caggtgtacc ttctgtgatc aacttaccgt
     7681 ttttcattgc taataaataa tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca
     7741 tcaagatcgt tgtttgtttc atttgattga tttcagtcaa taactgtagg atctctaatt
     7801 ggtgtgccgg atctaaaaat gttgtcggtt catccaagat caagatatct gtttcctgag
     7861 caagcgccat agcgatccag acacgctggg tttgtccacc agacagcgtt gcgatatccc
     7921 gcatacgaaa tccagctaga cctgtagcat ccaacgccca ctcaattgct tcatgatctt
     7981 gcgtgtccat gccactgaac agtttttgat aagggtatcg tccgtaagat actacttctt
     8041 ctactgtcat acctgtaggg tgttcactag tttgtgctaa taatgcaatt ttttttgcaa
     8101 gttcttttga actgtatgta gagatatcag agttattgac aatgatcttt cctgatttca
     8161 caggtagtat acgggtaaaa cttttcaata aagtagattt tccacttcca tttggcccaa
     8221 tcaaactagt gattttccct tcaggaatcg taacagacat gttttcaatg atttttttgt
     8281 tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt catttcaccc atcctttcgc
     8341 tgaaaatcat tctcaattat gacgctgttt caacatttcg tcaagcgcta ctttcatatc
     8401 tatagccaaa tgaagcggtt gacagcgttc gaaatatcag gaataataac caatgagaaa
     8461 gattctcaat tatgttagga ggaaagaaat gaaattaaaa acaaaagtat tcacattatc
     8521 tagcatattg ctgctggctg gagtactgag cgcttgctcc ccctcttcag ataaagaaac
     8581 agctacaact gccaattcga cgacttcatc caatagtact catacagtca cagatacact
     8641 tggacataaa gtagagattc caaacgcgcc caaacggatc atcggcagtt acttagaaga
     8701 ctatctgatt gcactagggg aaaaacctat cgcccagtgg actgttggca gtggttccat
     8761 tcaagattat ctgcaagatg atttaaagga tgtcccaacg atttcttatg accttcctta
     8821 tgagaaagtg ttaagctttg agcctgattt gctgttgatt tcttcttccg ctactgtcga
     8881 aggcgggaaa tacgaacaat atagcaaaat cgcgccaact tatgttgtaa aaaacggaac
     8941 agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga
     9001 ggcagaaaaa atcatttccg attatcaaaa atccatcaag aaaactcgag aagaactagc
     9061 agataaaatc gagaacaaga cagctgcagt gttgtgggtg accaacaact cagcctttat
     9121 ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac gatctggaat ttggtgttcc
     9181 tgatctagtg gaagaggtct ccaaaaaagc cacttctgac tggtctgctg tttcctctga
     9241 aaagctagct gaattagatg ctgactacat catccttgtg aacagtgaca aagatgctgc
     9301 catgttcaat gaaccaagct ggaagaatct aaaagcagtg acagacaatc atgtactgca
     9361 attcggacct gaatctagtt ggctatataa cggacctatt gccagtcagg aaatggtcaa
     9421 tgatatcaaa gaaaaactaa attgatttta actaaaaaaa gcatcttcac gattagtcgt
     9481 gagatgcttt ttttcttatt aacaaactag acgcatagat tttattcttc ttttgaagca
     9541 accctatttt ttactctttc ccatttttga aaacgatggg catatagatt ttcttgatcc
     9601 gttatcccct catactcctc tataagggcg aagtctgaaa aatcaatatt cccaatatac
     9661 gtatcgcctt caaacgtgct atcgatcaat gttcgccaga tccgaccagt gtaaggaaga
     9721 agttcttgaa aaatccgact accacccgaa acatagatat cttctgagat cgtttgacta
     9781 tacgacaaaa gttcatcgat cgaatgcatt acttccgcat tttctttcgt gcgaaaattt
     9841 ggttgcgttg tcaacacgat gatatggcga taaggaagtg aaagatcccc cattccttca
     9901 taggtctttc ttcccatcac taaaattcga tccattgtat gttcgcgaaa gaaacgcata
     9961 tcatttggca atcgccatgg taacaaacca tttttgccga tcagaccatt tttatcttgt
    10021 gcccacatgg aaataaacat attatccgct ccttatgatt ttagatgttt tgagataaaa
    10081 aaagtctcaa ttctttgacc agcaaggaat tgagaacttt aaaaacatta ttttgtttca
    10141 cggtgcaaag taactttgcg ttcgcgtggg caatattttt gtttttccaa acgatcagga
    10201 ttgttacgtt tgtttttgct tgttagatag ttacgttctt tacaagaagt acactctaaa
    10261 gtgatgttta cgcgcatgtt tttccctccc atttttacaa gaatcctcta agatgactct
    10321 cagacttgat tatcttacca tgattttttc tagattgcta gtgtttttca cgattttgtt
    10381 aagtaaaact acttgacgat aaatacaacg gttttacttc tcaaacaaaa tctttttctt
    10441 tttgtataaa tacccttaca cttgtttata tagattatgt tttttaataa agggggatac
    10501 taatatgcca tggaacatga aggactttcc tgcatcaatg aaaaacttag ataaattgac
    10561 aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat gaagggtacc ctgataaccg
    10621 tgcgatccca attgccattg atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga
    10681 aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc
    10741 tcgtgctgga aaactgcttg attcagatgt gatcgtagaa tatgaggaag aacaatggat
    10801 cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa aagaagcaat
    10861 cgaaaaagga aagcaagtag cacaaaacaa agaatcaact ttgttcattt ataaaaaaga
    10921 tgggacaaaa gaaaaagaaa tcgattattg aacatatgta aaggggttgt gacgaaaatc
    10981 gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc gaaccataag
    11041 tcataatatc tataaaacgg gaagagctgt tttttgaaat tgtttcttat cactcagtgt
    11101 taaacggtag gagaaaacct cttttttatt gatatttgat tttcatcaaa cagtcacata
    11161 gtcgattgct tctgcttgcg gaatcgtttc atctacaaag tagactttat accatgtcaa
    11221 gaaactgaag attgtccaga tttttctttg ctcttcttta cgtccttcgt gatggtcatc
    11281 tagcaacttc atgatttttt cttgatcaaa aaattctttt gcaaaatctg cttcgaataa
    11341 tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg gcacagggaa
    11401 tcctaatttc acacgattcg accattcttc cggaagatgt ttgttcgcag cttgtctgaa
    11461 aatatctttt gtgttgtttt gattcaataa aaacttactt ggtatttgtt gagccaattt
    11521 catgacttcg atatccaata aaggtactcg gacttccaaa gaacttgcca tagaaagttt
    11581 atctgccttt aacaagatat cttttggcat ccattgatga agatcgacat attgcatctt
    11641 attgacttca tctttgattc cttctgtttt ctcgtaatga acagtcatga tttcatccac
    11701 agatggtgct gtttgatatt ttggtgttaa cacttctgat gcttctgatt cttcgaacac
    11761 tcgagcatga ccgataaaat attcttttgc aggagcaagt gattcatata aatggatccg
    11821 tccatggaag tttttcattt ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg
    11881 taattttttc aatccttgag caatcacacg gatgaatttt gaattcgtat ggaatccata
    11941 cgtttggtat cccgcaaaca gttcatccgc cccttctcca gattggacta cccgcacact
    12001 ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc
    12061 atctaagtga tactggatca aagggaatgc tttgaacgac atgtctccgt cgatcactgc
    12121 tgctgtatta tctaagtcca atagttcggt tagttttctg gcttcaatcg cttcattata
    12181 ggttttatca tcaaacccga ttgagaaaga atgatctggt ttcaatacag aggttacata
    12241 actggaatca accccgctgg ataagaaaga accaacttct acatcactga ccgtatgtgc
    12301 ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt
    12361 ttcttttgtt tcaaaatctg catcccaata ttgctgaatg tctatttttc catctttata
    12421 cgtataataa tggccttcag gcaaacgata gacatctttg aagaatgttt cgccgtttaa
    12481 tggagaatat tggaaagtca tataaggttt caacgcttct ttgttcaatt ctttgttaaa
    12541 atcaggatga ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc cgttcatttc
    12601 tgcataataa tatggtttga tcccaaagtg gtctcttgca ccgaataact cattcttttc
    12661 attatcccaa atagcaaaag caaacattcc gcggatcttt tgcaataatt ctgttcccca
    12721 ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc
    12781 tgcaatcaat tcttctctca aaggctggta attatagatt tcgccattaa aaataatgat
    12841 ctttgtccga tcttcattgt aaataggctg agtaccgccc tctaagtcaa taatacttaa
    12901 tcttctgaat cctaaagcaa catgtttatc gatatattct cctgaactat taggtccacg
    12961 gtgaacgatt cgatccatca ttgtattaat aattgttttt ttgttgtcct tgtcgttaac
    13021 aaaacctaca ataccgcaca tttaactttc acatcctaat ataaaattac tgtttatttc
    13081 attcatatgc atcttatcat agaataaata gcggacgaat attttctctc ttacaatttc
    13141 gtcacgtgaa aacaaaagat gaaagtttct gtttttacag aaaataaagt cccaatatct
    13201 aatttaccat gttttctttt ttgaggctac cttattctct tcgtgttaaa aaaatattag
    13261 tggatgctat ttccaaaaaa accagcaaaa aaagacacga cctaaagatc gtgt
//
LOCUS       DACDID010000076        13261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDID010000076 DACDID010000000
VERSION     DACDID010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.076"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IV236_001693"
     CDS             complement(12..1535)
                     /locus_tag="IV236_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8771883.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IV236_001694"
     CDS             1787..2968
                     /locus_tag="IV236_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8771884.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV236_001695"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IV236_001695"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8771885.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IV236_001696"
     CDS             complement(5053..7764)
                     /locus_tag="IV236_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8771886.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IV236_001697"
     CDS             complement(7774..8475)
                     /locus_tag="IV236_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771887.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IV236_001698"
     CDS             8661..9260
                     /locus_tag="IV236_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8771888.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IV236_001699"
     CDS             9341..10354
                     /locus_tag="IV236_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8771889.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IV236_001700"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IV236_001700"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8771890.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IV236_001701"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IV236_001701"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8771891.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IV236_001702"
     CDS             11802..13175
                     /locus_tag="IV236_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8771892.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3766 a         2628 c         2456 g         4411 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 g
//
LOCUS       DACDID010000077        13242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDID010000077 DACDID010000000
VERSION     DACDID010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.077"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..1870
                     /gene="yfcC"
                     /locus_tag="IV236_001703"
     CDS             131..1870
                     /gene="yfcC"
                     /locus_tag="IV236_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8771893.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2027..3211
                     /gene="iadA"
                     /locus_tag="IV236_001704"
     CDS             2027..3211
                     /gene="iadA"
                     /locus_tag="IV236_001704"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8771894.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3322..3525
                     /locus_tag="IV236_001705"
     CDS             3322..3525
                     /locus_tag="IV236_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8771895.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3626..4288)
                     /locus_tag="IV236_001706"
     CDS             complement(3626..4288)
                     /locus_tag="IV236_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8771896.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4301..5803)
                     /locus_tag="IV236_001707"
     CDS             complement(4301..5803)
                     /locus_tag="IV236_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8771897.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6351..8030
                     /locus_tag="IV236_001708"
     CDS             6351..8030
                     /locus_tag="IV236_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8771898.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8157..8636
                     /locus_tag="IV236_001709"
     CDS             8157..8636
                     /locus_tag="IV236_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8771899.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8855..9535
                     /locus_tag="IV236_001710"
     CDS             8855..9535
                     /locus_tag="IV236_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8771900.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9543..10874
                     /locus_tag="IV236_001711"
     CDS             9543..10874
                     /locus_tag="IV236_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8771901.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10889..11851
                     /locus_tag="IV236_001712"
     CDS             10889..11851
                     /locus_tag="IV236_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8771902.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11962..12300
                     /locus_tag="IV236_001713"
     CDS             11962..12300
                     /locus_tag="IV236_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8771903.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12379..13086
                     /locus_tag="IV236_001714"
     CDS             12379..13086
                     /locus_tag="IV236_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8771904.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4212 a         2336 c         2904 g         3790 t
ORIGIN      
        1 aaaacaaaat agaaaaaagt gattttctaa taaattattc atgtggaaat gtcattttta
       61 ctgtataatt catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga
      121 agaaataaat ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt
      181 atcgacaccc catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac
      241 ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg
      301 cgaaacaagt actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga
      361 taagtcgtat gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa
      421 actggatgtt ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac
      481 gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga
      541 aaatatctat gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga
      601 ttttggcggc tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta
      661 tggatctgct gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat
      721 tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact
      781 ggagcgttta gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg
      841 gatggcagaa gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg
      901 atatgactcg atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac
      961 ctcttggatg agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt
     1021 gtctggtgcg acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta
     1081 tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc
     1141 agatgattat ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct
     1201 tgggcataaa ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt
     1261 gactcaaaaa ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt
     1321 tgctggaatc attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc
     1381 ttctttccaa tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa
     1441 agggattctt cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat
     1501 tttgcatggg atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat
     1561 gtacatcttc caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt
     1621 gactatgcca atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt
     1681 tcttgcttat cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt
     1741 gattggtgtg ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa
     1801 gatgcaagga ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg
     1861 tctgcagtag tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc
     1921 caagaaccgt cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt
     1981 tttaagatcc tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg
     2041 acaaattgaa gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg
     2101 gaagattttg gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga
     2161 actttcaggt gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt
     2221 aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt
     2281 gactacagct ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga
     2341 tatgactgcg ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt
     2401 atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt
     2461 tttgacaatt gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc
     2521 tcagccaagt tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt
     2581 atccggaaaa gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct
     2641 attgacacga atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc
     2701 aaatcgcaca ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat
     2761 tgacttcact gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt
     2821 cagcaaagca ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac
     2881 ctcagatgga caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg
     2941 agtaggaagt gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat
     3001 ccctttggaa atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga
     3061 taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac
     3121 gcttgatatt gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa
     3181 agtatggggg actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa
     3241 acgagcgttc tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac
     3301 aaactaagag gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat
     3361 aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa
     3421 acagaaggga aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc
     3481 gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact
     3541 aattgaatca agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt
     3601 ttttatttga tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta
     3661 gttcaaagac ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac
     3721 agcgattttg ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt
     3781 catctagatt tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac
     3841 gaacgatagc gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta
     3901 atatttcatc aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt
     3961 gcttggtctt agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa
     4021 gcaagttgta agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga
     4081 ttttgttcag tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag
     4141 aaataagcga aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt
     4201 ttccagcttc gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc
     4261 aaaatcccaa ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc
     4321 ttgggtttaa tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa
     4381 atgctactcc ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga
     4441 cttgatcaga aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc
     4501 cttgttcttg tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg
     4561 tggtttctgt ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc
     4621 taagcgaagc aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga
     4681 gaatgatttt acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct
     4741 cccgaatggt aatcataatg atcagagtaa gtatgataat accggctaca gccactagta
     4801 aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca
     4861 tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga
     4921 gaaactcatc cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat
     4981 cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg
     5041 gattcatgaa gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc
     5101 ctttgatcgt catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat
     5161 ctccgactgc caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc
     5221 ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat
     5281 tactattttg tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg
     5341 gattcccgcc cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt
     5401 cacttgaatc agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac
     5461 tttcaaagga ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg
     5521 aaactggtgt aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg
     5581 aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg
     5641 ctccaacttc tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc
     5701 cagctagcac aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc
     5761 attttacttt tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta
     5821 gtagatgcat ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg
     5881 cacaccatca acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg
     5941 tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa
     6001 aagaaatcct agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga
     6061 tactagcgat ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag
     6121 tattctggct atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa
     6181 aatgcccatc caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata
     6241 agttgagaaa tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag
     6301 aagaaaatcg acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca
     6361 ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag
     6421 tgggcgagga tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg
     6481 ggatcgacgt cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg
     6541 gtgtcttttt aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa
     6601 aaatttctgt accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca
     6661 gtcgtaatga agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa
     6721 tagactttgg tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag
     6781 tggggatcaa tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg
     6841 accaaacggc tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag
     6901 aaggagtctt agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt
     6961 ctgaagccca aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca
     7021 tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa
     7081 aagcacaacg aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga
     7141 tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga
     7201 aaaactatga tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca
     7261 agtctctgca aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt
     7321 tggtttatat cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag
     7381 atatcattta tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg
     7441 gccatgccaa tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg
     7501 tgccagttca aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag
     7561 ggatgccata caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca
     7621 agcgtatgag tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca
     7681 taggtgatat cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt
     7741 tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta
     7801 cttcacgggg attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata
     7861 tgattgaaac aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa
     7921 agcaagacat ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag
     7981 tgatcttgcc agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag
     8041 taagatgaac ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt
     8101 tttgaataca gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga
     8161 tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg
     8221 attttagtca tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc
     8281 cagctttgat atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat
     8341 tgattccttt gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta
     8401 aagttggttt tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa
     8461 acacccctat tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt
     8521 taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa
     8581 aagtagccga tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga
     8641 cgagaagaga agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact
     8701 tggtgcatcc tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg
     8761 aaaatatatt tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat
     8821 aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc
     8881 ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg
     8941 aaatccattg acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca
     9001 tggtcatagt gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat
     9061 tatcagtatt gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat
     9121 gaatatcggt ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca
     9181 tagttcaagc tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat
     9241 tattcaagca gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct
     9301 aggattgctt gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta
     9361 tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt
     9421 tccaatccat tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt
     9481 actggaagat agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga
     9541 aaatggcgac aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc
     9601 gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg
     9661 caatcaaaga tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc
     9721 gtatcgaacg caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga
     9781 tcattgaagg ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg
     9841 tcatcggcgc gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga
     9901 tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga
     9961 tcacaggagg attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc
    10021 cgatcttacg cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa
    10081 gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa
    10141 agactcatta tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa
    10201 cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg
    10261 caaaagatgt tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac
    10321 caatcagtgt aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg
    10381 ggctagaagt catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc
    10441 aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt
    10501 cggatcagat atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg
    10561 aaaaccctaa gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt
    10621 cttttggcgt acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc
    10681 aaaaacgaaa tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg
    10741 aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg
    10801 atatcgaagt gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa
    10861 tggaacgttc ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat
    10921 aaaaaaatac gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg
    10981 atcacaagta ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca
    11041 agtaggtgaa acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt
    11101 cttctaccaa ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga
    11161 tcaaagatat cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc
    11221 ttatggagat cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga
    11281 atttgctttg atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta
    11341 tgcaggaatc gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt
    11401 agagattgct tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat
    11461 tgaacaaatg ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt
    11521 ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat
    11581 cgatgattca gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc
    11641 ttgggggatt tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg
    11701 gccgatcatc aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg
    11761 tgcgaatgca aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg
    11821 ccaatcgtat aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag
    11881 taaaaatcac aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa
    11941 agattttaaa ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac
    12001 acatatgaag atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa
    12061 gaacaagaaa ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg
    12121 ttagagggcg atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc
    12181 attaaacaag gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat
    12241 tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa
    12301 agaatattca aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg
    12361 aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt
    12421 cttttggtca ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact
    12481 ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa
    12541 tatatattac aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc
    12601 acagataact acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct
    12661 acttcagttg ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct
    12721 tcgggttatg tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg
    12781 atgatttcgt tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc
    12841 aatatcggga ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc
    12901 gaatttaacg cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa
    12961 gaagaagtac cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca
    13021 gcgctttcga cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga
    13081 gattaaaata aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc
    13141 tgattgaata ccatttattt ggaataaggg ctctttgtca ataaggactg atgggttgta
    13201 caaaatcaaa tctgggtcag aatgaacctc attctggctc ag
//
LOCUS       DACDID010000078        12812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDID010000078 DACDID010000000
VERSION     DACDID010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.078"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..731
                     /locus_tag="IV236_001715"
     CDS             9..731
                     /locus_tag="IV236_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8771905.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="IV236_001716"
     CDS             789..2828
                     /locus_tag="IV236_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8771906.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="IV236_001717"
     CDS             complement(2937..4088)
                     /locus_tag="IV236_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8771907.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="IV236_001718"
     CDS             complement(4242..4772)
                     /locus_tag="IV236_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771908.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="IV236_001719"
     CDS             complement(4871..5221)
                     /locus_tag="IV236_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8771909.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IV236_001720"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="IV236_001720"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8771910.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IV236_001721"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="IV236_001721"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8771911.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="IV236_001722"
     CDS             complement(7624..8286)
                     /locus_tag="IV236_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8771912.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="IV236_001723"
     CDS             8470..10116
                     /locus_tag="IV236_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8771913.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..10235
                     /locus_tag="IV236_001724"
     CDS             10107..10235
                     /locus_tag="IV236_001724"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771914.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10202..11266
                     /gene="uxuA"
                     /locus_tag="IV236_001725"
     CDS             10202..11266
                     /gene="uxuA"
                     /locus_tag="IV236_001725"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8771915.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11374..>12812)
                     /locus_tag="IV236_001726"
     CDS             complement(11374..>12812)
                     /locus_tag="IV236_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8771916.1"
                     /translation="DHFHMSESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEG
                     IDPAYCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINA
                     FLALYGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMN
                     TFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDI
                     LRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFC
                     TVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIE
                     QFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKD
                     LIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKV
                     IMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4288 a         2360 c         2382 g         3782 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tcacaggaag aaaagattac cttagatgaa atcagacaga ttcctgcggt
    10261 caaagggatt gtaggcacat tgatggatat accagtagga gaggtatggc ccagggagag
    10321 gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta acactaaaag taattgaaag
    10381 cgtcaatgtg catgatgcca ttaaaattgg attaccttcc cgtgagcagt atatcgaaaa
    10441 ctataagcag acaatccgaa acttggcaga atatggaata gaagtcatct gctataattt
    10501 tatgcctgta tttgactggg tgaaatctga ccttgactac cgcttagaag acggctcatc
    10561 cacgttagct tttatatctg atgatattcc agatgatcca agagaaatcg tcgcgcgcat
    10621 cgaacattcc agcaatgaat ttgaattgcc gggatgggaa cctgagcgct tagctcatat
    10681 caaatccttg cttgaagcct atgcatcagt agacgaagaa aaacttcgag aaaattttgc
    10741 ctattttctc caatcaatca tcccaacatg tgaagaggta ggggtcaaaa tggctgtaca
    10801 tccagatgat ccgccatact cgatttttgg attacctcga atcgtaaaga atcgagaaga
    10861 tttagattgg ttgtgccgca tagtagacag cccttataac ggaatcactc tttgtacagg
    10921 gagtattgca gaagatccag aaaatgatgt atatgggatt ttagcagaat tttgtcaaag
    10981 agatcgaatc ccatttgcac atgttcgaaa tattaaattt ttagctaatc gagatttcta
    11041 tgaggcaccg cataaatccg aatacggttc attagacatg taccgcatac taaaaacaat
    11101 gtatgacaac ggatttaacg aatacatccg tcctgatcat ggaagaatga tctggaatga
    11161 gacaggaaga ccaggatacg gcttatatga tcgagcatta ggtgctgttt atctaaatgg
    11221 attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat aattagtgaa aaaaactgta
    11281 attttattat gtaaataaga aggatgttca aattcttgat acataaatcc ggaggttgtt
    11341 aataagcatt cggatttatg tatcttattt ggtctaggaa gtaacttgat tttctttttt
    11401 cgattgatcc atgagtgctt cttcttttaa ttcaaagata tattctgaga tcctcataat
    11461 cacttttttc cagtcttctg gtgtgatgat tccttttaaa atatagattt ttttgatatg
    11521 agaaggcaat tgtttcaaag ggcagtcgct gataacgatg ttgcagttct ccataaaatc
    11581 attttgtatt ttgatcgatt catttgaaaa aatttgttct atcttaacga tcagatcttt
    11641 actgatataa aaatcacgcg tatagttcac accaatcaca attcgcggct gttttttgta
    11701 gcgatcatac aaatgtacta ggtcttcaac catccattgc acattttcct tttctaaaat
    11761 aactggtaac aagtgttgat tttgttctct gaaatccata taaaattgtt caatggcggt
    11821 ttggacttct ttcaacacgc tatgttcttg ataaagcatg aaagtaggaa ggggcgtatt
    11881 tggttgattg aagttaagaa atccattata aagtttattt cgtgcgaatg ccaatgagta
    11941 aagaatcttt tctttttcag gtataaacaa atcaaataca gtacaaaagg actccaccat
    12001 ttctcttgaa taaagaacat gaggaacgtt tgcttttttt aaaagtttgt attgctccaa
    12061 gcttgtttct tccgattcga ttttcgtgat agaaagtcga gctaaaatat tcactaagat
    12121 gcgttgttcg cgacaaattg gcagattagg tcgcaaaata tctacagagg gatcaataaa
    12181 aaagtcaata tattcatact ctgtgaattc tttttgtacg tctttcacag aagttgcagg
    12241 aaaattctct ttacatattt ttaatagaaa atataattta tctatcatac cgctatttag
    12301 tcggtcaagt acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc
    12361 ttcatcataa aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc
    12421 atattgaatt tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat
    12481 agacaaaccg tacaatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga
    12541 ggcactatag tttgtttgct gacttaaact gataatcgaa tttgtctgac agctgaacaa
    12601 agaagtcaag atctgataac tgactgattc gttgcagtat cttccaaata gacgataata
    12661 gcagtaagcc ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat
    12721 atgcagttct gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa
    12781 aaaatttgat tcagacatat ggaaatgatc tg
//
LOCUS       DACDID010000079        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDID010000079 DACDID010000000
VERSION     DACDID010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.079"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV236_001727"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV236_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8771917.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV236_001728"
     CDS             complement(322..891)
                     /locus_tag="IV236_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8771918.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV236_001729"
     CDS             1077..1979
                     /locus_tag="IV236_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8771919.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV236_001730"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV236_001730"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8771920.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV236_001731"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV236_001731"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8771921.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV236_001732"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV236_001732"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8771922.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV236_001733"
     CDS             8547..8732
                     /locus_tag="IV236_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8771923.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV236_001734"
     CDS             complement(8794..9990)
                     /locus_tag="IV236_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8771924.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV236_001735"
     CDS             complement(10160..11596)
                     /locus_tag="IV236_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8771925.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV236_001736"
     CDS             11889..12698
                     /locus_tag="IV236_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8771926.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDID010000080        12687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDID010000080 DACDID010000000
VERSION     DACDID010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.080"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IV236_001737"
     CDS             297..1406
                     /locus_tag="IV236_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8771927.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IV236_001738"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IV236_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IV236_001739"
     CDS             2401..3225
                     /locus_tag="IV236_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771928.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IV236_001740"
     CDS             3257..4567
                     /locus_tag="IV236_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8771929.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IV236_001741"
     CDS             4715..5974
                     /locus_tag="IV236_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8771930.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IV236_001742"
     CDS             5984..6856
                     /locus_tag="IV236_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771931.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IV236_001743"
     CDS             6868..7698
                     /locus_tag="IV236_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771932.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IV236_001744"
     CDS             7738..9921
                     /locus_tag="IV236_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8771933.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IV236_001745"
     CDS             9982..11202
                     /locus_tag="IV236_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8771934.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IV236_001746"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IV236_001746"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8771935.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
BASE COUNT         4284 a         1909 c         2536 g         3958 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tccttta
//
LOCUS       DACDID010000081        12514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDID010000081 DACDID010000000
VERSION     DACDID010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.081"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..299)
                     /locus_tag="IV236_001747"
     CDS             complement(<1..299)
                     /locus_tag="IV236_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771936.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIW"
     gene            complement(373..753)
                     /locus_tag="IV236_001748"
     CDS             complement(373..753)
                     /locus_tag="IV236_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8771937.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            835..2010
                     /locus_tag="IV236_001749"
     CDS             835..2010
                     /locus_tag="IV236_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8771938.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2087..2671)
                     /locus_tag="IV236_001750"
     CDS             complement(2087..2671)
                     /locus_tag="IV236_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8771939.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2872..3870
                     /locus_tag="IV236_001751"
     CDS             2872..3870
                     /locus_tag="IV236_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8771940.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            3982..4491
                     /locus_tag="IV236_001752"
     CDS             3982..4491
                     /locus_tag="IV236_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8771941.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4521..5180)
                     /locus_tag="IV236_001753"
     CDS             complement(4521..5180)
                     /locus_tag="IV236_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8771942.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5182..5946)
                     /locus_tag="IV236_001754"
     CDS             complement(5182..5946)
                     /locus_tag="IV236_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8771943.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(5954..6631)
                     /locus_tag="IV236_001755"
     CDS             complement(5954..6631)
                     /locus_tag="IV236_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8771944.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6668..8053)
                     /locus_tag="IV236_001756"
     CDS             complement(6668..8053)
                     /locus_tag="IV236_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8771945.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8263..8347)
                     /gene="ffs"
                     /locus_tag="IV236_001757"
     ncRNA           complement(8263..8347)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV236_001757"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8370..8885)
                     /gene="tadA"
                     /locus_tag="IV236_001758"
     CDS             complement(8370..8885)
                     /gene="tadA"
                     /locus_tag="IV236_001758"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8771946.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            8992..9849
                     /locus_tag="IV236_001759"
     CDS             8992..9849
                     /locus_tag="IV236_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8771947.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(9930..10265)
                     /locus_tag="IV236_001760"
     CDS             complement(9930..10265)
                     /locus_tag="IV236_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771948.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10332..11702)
                     /locus_tag="IV236_001761"
     CDS             complement(10332..11702)
                     /locus_tag="IV236_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8771949.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11780..12316)
                     /locus_tag="IV236_001762"
     CDS             complement(11780..12316)
                     /locus_tag="IV236_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8771950.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3737 a         2544 c         2088 g         4145 t
ORIGIN      
        1 tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca
       61 aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg
      121 ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt
      181 ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat
      241 tttttagtac catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa
      301 atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa
      361 aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat
      421 ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa
      481 ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa
      541 actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac
      601 ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag
      661 tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt
      721 tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat
      781 tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc
      841 gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct
      901 gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct
      961 cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc
     1021 actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg
     1081 gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca
     1141 gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca
     1201 ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa
     1261 aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta
     1321 aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa
     1381 ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc
     1441 catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat
     1501 ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc
     1561 aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta
     1621 gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat
     1681 gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca
     1741 gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact
     1801 tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact
     1861 cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat
     1921 gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga
     1981 atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc
     2041 ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt
     2101 caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg
     2161 cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat
     2221 ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac
     2281 cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc
     2341 ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt
     2401 cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc
     2461 cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat
     2521 cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag
     2581 ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt
     2641 cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc
     2701 ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat
     2761 ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg
     2821 agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc
     2881 tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga
     2941 atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca
     3001 actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt
     3061 gaattaatcg atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa
     3121 gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc
     3181 atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa
     3241 ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat
     3301 ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat
     3361 tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa
     3421 tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact
     3481 ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa
     3541 aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga
     3601 ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt
     3661 actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt
     3721 acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg
     3781 aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc
     3841 ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa
     3901 acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa
     3961 aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga
     4021 gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta
     4081 caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg
     4141 ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca
     4201 gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac
     4261 ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg
     4321 aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat
     4381 ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc
     4441 agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa
     4501 aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt
     4561 tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag
     4621 ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat
     4681 aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct
     4741 atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc
     4801 aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa
     4861 gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg
     4921 gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg
     4981 agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa
     5041 tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca
     5101 ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac
     5161 tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt
     5221 agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa
     5281 agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat
     5341 gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc
     5401 tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga
     5461 ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat
     5521 gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata
     5581 attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc
     5641 taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc
     5701 tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg
     5761 aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt
     5821 ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc
     5881 cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata
     5941 gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta
     6001 cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc
     6061 gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat
     6121 acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc
     6181 cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt
     6241 ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt
     6301 ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat
     6361 cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc
     6421 gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt
     6481 tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg
     6541 tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa
     6601 cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac
     6661 cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa
     6721 attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa
     6781 acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct
     6841 tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa
     6901 aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt
     6961 tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt
     7021 gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt
     7081 cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg
     7141 cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg
     7201 gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga taactattta
     7261 tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat
     7321 gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt
     7381 actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat
     7441 gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat
     7501 cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga
     7561 ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa
     7621 gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga
     7681 ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca
     7741 ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta
     7801 cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat
     7861 tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat
     7921 ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa
     7981 accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca
     8041 ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt
     8101 tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag
     8161 tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta
     8221 taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg
     8281 cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt
     8341 cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt
     8401 aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg
     8461 tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga
     8521 ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct
     8581 acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt
     8641 gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt
     8701 tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact
     8761 tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt
     8821 gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt
     8881 accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg
     8941 ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg
     9001 gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg
     9061 gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct
     9121 caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac
     9181 ccatggtatc gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg
     9241 atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt
     9301 ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta
     9361 gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct
     9421 ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc
     9481 accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat
     9541 ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa
     9601 gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa
     9661 atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa
     9721 acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct
     9781 ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa
     9841 cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg
     9901 aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc
     9961 cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt
    10021 tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga
    10081 ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt
    10141 actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact
    10201 gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt
    10261 ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg
    10321 aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt
    10381 acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc
    10441 ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc
    10501 aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat
    10561 cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga
    10621 gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta
    10681 gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt
    10741 tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa
    10801 gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt
    10861 ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa
    10921 ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt
    10981 tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt
    11041 attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc
    11101 cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca
    11161 accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt
    11221 gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt
    11281 ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg
    11341 gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca
    11401 gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg
    11461 aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt
    11521 aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac
    11581 cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg
    11641 cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc
    11701 attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc
    11761 tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat
    11821 ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat
    11881 catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt
    11941 tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt
    12001 ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca
    12061 ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat
    12121 aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat
    12181 aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca
    12241 ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg
    12301 ttactctccc catcattctt acctccgtct cttttcatct acccataaat aaataacata
    12361 aataattcta aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa
    12421 tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat
    12481 agaataaaca aggtaacaaa aaaattaaat aacg
//
LOCUS       DACDID010000082        12074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDID010000082 DACDID010000000
VERSION     DACDID010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.082"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IV236_001763"
     CDS             complement(221..1423)
                     /locus_tag="IV236_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8771951.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IV236_001764"
     CDS             complement(1426..1620)
                     /locus_tag="IV236_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8771952.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IV236_001765"
     CDS             complement(1667..2515)
                     /locus_tag="IV236_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8771953.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IV236_001766"
     CDS             complement(2720..3070)
                     /locus_tag="IV236_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771954.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IV236_001767"
     CDS             3665..4111
                     /locus_tag="IV236_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8771955.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IV236_001768"
     CDS             4735..5727
                     /locus_tag="IV236_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8771956.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IV236_001769"
     CDS             5743..6627
                     /locus_tag="IV236_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8771957.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IV236_001770"
     CDS             6676..8283
                     /locus_tag="IV236_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8771958.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IV236_001771"
     CDS             8284..9768
                     /locus_tag="IV236_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8771959.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IV236_001772"
     CDS             9945..10937
                     /locus_tag="IV236_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8771960.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IV236_001773"
     CDS             10989..11858
                     /locus_tag="IV236_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8771961.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3748 a         1969 c         2494 g         3863 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tact
//
LOCUS       DACDID010000083        11837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDID010000083 DACDID010000000
VERSION     DACDID010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.083"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV236_001774"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV236_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8771962.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV236_001775"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV236_001775"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8771963.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IV236_001776"
     CDS             complement(2169..2906)
                     /locus_tag="IV236_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8771964.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IV236_001777"
     CDS             complement(2997..3524)
                     /locus_tag="IV236_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8771965.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IV236_001778"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IV236_001778"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8771966.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IV236_001779"
     CDS             complement(4401..4886)
                     /locus_tag="IV236_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8771967.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IV236_001780"
     CDS             complement(5039..5236)
                     /locus_tag="IV236_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771968.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV236_001781"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV236_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8771969.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV236_001782"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV236_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8771970.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IV236_001783"
     CDS             9211..9423
                     /locus_tag="IV236_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8771971.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IV236_001784"
     CDS             9425..11104
                     /locus_tag="IV236_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8771972.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IV236_001785"
     CDS             complement(11358..11558)
                     /locus_tag="IV236_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8771973.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11837)
                     /locus_tag="IV236_001786"
     CDS             complement(11708..>11837)
                     /locus_tag="IV236_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HAP8771974.1"
                     /translation="DMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREEGMMEEK"
BASE COUNT         3421 a         2562 c         2059 g         3795 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcc
//
LOCUS       DACDID010000084        11485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDID010000084 DACDID010000000
VERSION     DACDID010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.084"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV236_001787"
     CDS             <1..391
                     /locus_tag="IV236_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771975.1"
                     /translation="REYFGIEYSSNLGDVLFQFSQTLARFIPTEVSEKKNEDQKEAMC
                     FSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYERLGSDTNL
                     SFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            463..858
                     /locus_tag="IV236_001788"
     CDS             463..858
                     /locus_tag="IV236_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8771976.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            842..3295
                     /locus_tag="IV236_001789"
     CDS             842..3295
                     /locus_tag="IV236_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8771977.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3292..5319
                     /locus_tag="IV236_001790"
     CDS             3292..5319
                     /locus_tag="IV236_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771978.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5316..6338
                     /locus_tag="IV236_001791"
     CDS             5316..6338
                     /locus_tag="IV236_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8771979.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6355..7284
                     /locus_tag="IV236_001792"
     CDS             6355..7284
                     /locus_tag="IV236_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8771980.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7529..7645
                     /locus_tag="IV236_001793"
     CDS             7529..7645
                     /locus_tag="IV236_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8771981.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            7661..8464
                     /locus_tag="IV236_001794"
     CDS             7661..8464
                     /locus_tag="IV236_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8771982.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            8639..8935
                     /locus_tag="IV236_001795"
     CDS             8639..8935
                     /locus_tag="IV236_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771983.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8942..10894
                     /locus_tag="IV236_001796"
     CDS             8942..10894
                     /locus_tag="IV236_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8771984.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(10966..11463)
                     /gene="dfrG"
                     /locus_tag="IV236_001797"
     CDS             complement(10966..11463)
                     /gene="dfrG"
                     /locus_tag="IV236_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8771985.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         3916 a         1951 c         2238 g         3380 t
ORIGIN      
        1 aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat ttcaatttag
       61 ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg aagaccaaaa
      121 agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa aaaaatattg
      181 tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt
      241 taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag ggtcagatac
      301 caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg aaggtattat
      361 tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca atcacctatt
      421 ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa aataagaagt
      481 tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt acaactccct
      541 ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt cgtcatactc
      601 tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata cttagggatt
      661 cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa accctatgga
      721 tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc tggcaaacaa
      781 atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag tgaacaacta
      841 gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg atggcgaagt
      901 attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg atgaaaaaat
      961 tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca agattcatgc
     1021 tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga aaaaagaaat
     1081 cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg aagcgttaat
     1141 ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta aactctacct
     1201 caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag catttgcgga
     1261 attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc ctatggcgga
     1321 aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca gacgctttca
     1381 gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt acgggcaaac
     1441 aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag ataaaaaagt
     1501 gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg
     1561 ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta caattaatag
     1621 tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc aacagcagtt
     1681 tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa aagccttatc
     1741 taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat gggaaagcga
     1801 caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat tagaaagtaa
     1861 tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg tatcagcaaa
     1921 gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg atgatctgtc
     1981 tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat ttattccctc
     2041 aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt tggctggtct
     2101 tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg gttacaatct
     2161 cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa ttaaaggctc
     2221 tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa aatcattctc
     2281 taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca ttgttgaccc
     2341 aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg aaatcaatat
     2401 tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg tgattttaaa
     2461 actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa caggtatttc
     2521 cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg taactcaaag
     2581 caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata cgcccatcag
     2641 caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc atctgttatt
     2701 cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata ttattcaagt
     2761 ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta ccatggaatt
     2821 attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta tccactctga
     2881 ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac aagtggcaca
     2941 aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg caggggtcta
     3001 tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata atattggctt
     3061 gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag aattttttgg
     3121 tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa atggacaatg
     3181 tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga tttttgaaga
     3241 gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag aatgacacaa
     3301 aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg cgttatttta
     3361 cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga tgaaaccatt
     3421 gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga ttattatgtg
     3481 gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca atcggtgatg
     3541 tatggattgt atgccattac taattttatt tggacaattt ctctttatct atcgaacgcc
     3601 acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac atcccaagca
     3661 attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc cagttcaggg
     3721 ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt tgcctataca
     3781 ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt tgtcgtggtg
     3841 tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa aatcaatgat
     3901 ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt tcttcctaac
     3961 acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt ctctgttcaa
     4021 gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc gttaggggaa
     4081 gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga agatagagaa
     4141 aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct accgaagacg
     4201 atttccagat taggaacagt cttctttttg tttgtattca atattggtat ctcgattttt
     4261 gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat tttcgctatg
     4321 tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat gggcaggcat
     4381 gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt gattattacc
     4441 gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc ctttttcatg
     4501 attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg cgatttaatg
     4561 agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt gtttcgcaga
     4621 cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag taagattttt
     4681 cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa tcaaaccaat
     4741 aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac agcactttcc
     4801 aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac agcccatgta
     4861 ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca gatacaggcg
     4921 acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac tcataatgta
     4981 acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca gcgtatgaac
     5041 cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga taaagcaaaa
     5101 gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca gcatgttcgc
     5161 aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga aaatcatcag
     5221 cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa tcagaggaag
     5281 ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata acagtgattt
     5341 gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt ttctttgcag
     5401 atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc ggtgtgaccg
     5461 tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag gaatatggga
     5521 ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc ggtacagcag
     5581 aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg agtaccgaag
     5641 agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca gaacaacaag
     5701 gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt ttaaattatg
     5761 tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct aaagagaagt
     5821 cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat ggtggttggc
     5881 gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta accgacactt
     5941 ccccaacaga atttgatgat gaaaccgtgc aagtcattat ggacgaagcc ttgaaatacg
     6001 aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat tgtagtggct
     6061 taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc caagatcagt
     6121 atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt atcttctttc
     6181 attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta gagggaaatc
     6241 gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt tattggcaag
     6301 accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa tcaaatgatt
     6361 ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa accgagcaag
     6421 caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt tgttttatgg
     6481 gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc cattgacact
     6541 catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa ccaagtagaa
     6601 agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca agaagccatt
     6661 gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt attaaatgaa
     6721 gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca ggtgtggcag
     6781 gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca agttattacc
     6841 gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca tatagatgaa
     6901 tcagataata tggttattat caaaaatccg acaatgagta agaaacctca aaaatcggac
     6961 taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat ggacgaaatc
     7021 actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa agaattgact
     7081 tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt cgaggaattg
     7141 gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc tgtgaaatat
     7201 ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt agaaaaacaa
     7261 gataattgga agattgtaaa ataacaaata ttggtacatg attacagata ctttgtaatc
     7321 atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt acgtgctatt
     7381 atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct taaataaact
     7441 gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc ttagtttttt
     7501 gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga atatctgtat
     7561 gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga
     7621 tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg
     7681 agttttagct catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag
     7741 tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct
     7801 tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa
     7861 tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg
     7921 ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc
     7981 acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat
     8041 caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa
     8101 actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac
     8161 gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga
     8221 gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag
     8281 atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat
     8341 tgataacctt atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc
     8401 tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata
     8461 ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat
     8521 tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt taatttacac
     8581 cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta ttgtaggaat
     8641 gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc attttcctat
     8701 gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa tatcagagca
     8761 agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa gcgaagcagt
     8821 ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga ttgccaacaa
     8881 gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac
     8941 aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg ttaaaattcc
     9001 gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa ctccaaaacg
     9061 atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat ggaacggttc
     9121 tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt ttgggaaaga
     9181 acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga ctggtagttc
     9241 caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca gaggtgtatc
     9301 gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct
     9361 tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata aagaaggctg
     9421 ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa gggggaaaag
     9481 taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta atgttctacc
     9541 aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc caatgttcgc
     9601 taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag gaattggagt
     9661 tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt ttttagaacg
     9721 atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta atacggacaa
     9781 gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg
     9841 gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct tatcaagata
     9901 tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat taaatgaaga
     9961 tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg ataggttaga
    10021 ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga atgcttatct
    10081 tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa atgttgatgc
    10141 tccttatact acaactgctc actattggtt aattaaacaa atggttaatg ccgacgaatg
    10201 gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag tattcacgaa
    10261 agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga cgaaaagcag
    10321 aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt ggggagatat
    10381 cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa ctgaattact
    10441 aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat atcgcaaata
    10501 tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt attcagttga
    10561 ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt
    10621 taatgaccat tcgaccaata gctttattca acagataaga cgttatatat catctcttga
    10681 aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta acttcaatcc
    10741 gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt gtcgcccttt
    10801 taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta cagataaaca
    10861 atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag taatcccaag
    10921 ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac tcaataagtt
    10981 ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct ttatcccttt
    11041 ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag tatctccttc
    11101 gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa acaaattata
    11161 aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc
    11221 ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat
    11281 aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat
    11341 tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg
    11401 aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt
    11461 catttataac acagcctttt taact
//
LOCUS       DACDID010000085        11458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDID010000085 DACDID010000000
VERSION     DACDID010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.085"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..605)
                     /locus_tag="IV236_001798"
     CDS             complement(60..605)
                     /locus_tag="IV236_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8771986.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="IV236_001799"
     CDS             complement(617..1309)
                     /locus_tag="IV236_001799"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8771987.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /locus_tag="IV236_001800"
     CDS             complement(1328..1612)
                     /locus_tag="IV236_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771988.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="IV236_001801"
     CDS             complement(1625..2188)
                     /locus_tag="IV236_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8771989.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="IV236_001802"
     CDS             2416..3078
                     /locus_tag="IV236_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8771990.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="IV236_001803"
     CDS             3202..4398
                     /locus_tag="IV236_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8771991.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="IV236_001804"
     CDS             complement(4499..5875)
                     /locus_tag="IV236_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8771992.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="IV236_001805"
                     /pseudo
     CDS             complement(5912..6778)
                     /locus_tag="IV236_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6867..7892)
                     /locus_tag="IV236_001806"
     CDS             complement(6867..7892)
                     /locus_tag="IV236_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8771993.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="IV236_001807"
     CDS             complement(7910..8584)
                     /locus_tag="IV236_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8771994.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8768..9661)
                     /locus_tag="IV236_001808"
     CDS             complement(8768..9661)
                     /locus_tag="IV236_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8771995.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9735..11432)
                     /locus_tag="IV236_001809"
     CDS             complement(9735..11432)
                     /locus_tag="IV236_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8771996.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3389 a         2519 c         1965 g         3585 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctggtttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccacc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccctatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
     8761 tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc
     8821 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
     8881 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc
     8941 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
     9001 ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt
     9061 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
     9121 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
     9181 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
     9241 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg
     9301 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     9361 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     9421 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     9481 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     9541 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     9601 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     9661 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     9721 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     9781 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     9841 ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg
     9901 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     9961 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
    10021 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
    10081 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
    10141 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
    10201 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
    10261 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
    10321 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
    10381 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
    10441 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa
    10501 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
    10561 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
    10621 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
    10681 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
    10741 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
    10801 ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg
    10861 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
    10921 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
    10981 gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt
    11041 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
    11101 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
    11161 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
    11221 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
    11281 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
    11341 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
    11401 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DACDID010000086        11164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDID010000086 DACDID010000000
VERSION     DACDID010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.086"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV236_001810"
     CDS             6..305
                     /locus_tag="IV236_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8771997.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV236_001811"
     CDS             378..1739
                     /locus_tag="IV236_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8771998.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV236_001812"
     CDS             1761..2021
                     /locus_tag="IV236_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8771999.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV236_001813"
     CDS             2035..2787
                     /locus_tag="IV236_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8772000.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV236_001814"
     CDS             complement(2881..3486)
                     /locus_tag="IV236_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8772001.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV236_001815"
     CDS             complement(3539..4513)
                     /locus_tag="IV236_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8772002.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV236_001816"
     CDS             complement(4891..5670)
                     /locus_tag="IV236_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8772003.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV236_001817"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV236_001817"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8772004.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV236_001818"
     CDS             7161..8558
                     /locus_tag="IV236_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8772005.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV236_001819"
     CDS             8639..9079
                     /locus_tag="IV236_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8772006.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV236_001820"
     CDS             9227..9553
                     /locus_tag="IV236_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8772007.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV236_001821"
     CDS             9554..10303
                     /locus_tag="IV236_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772008.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV236_001822"
     CDS             complement(10383..10838)
                     /locus_tag="IV236_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8772009.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3545 a         1955 c         2293 g         3371 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 atta
//
LOCUS       DACDID010000087        11114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDID010000087 DACDID010000000
VERSION     DACDID010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.087"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            76..1335
                     /gene="lysA"
                     /locus_tag="IV236_001823"
     CDS             76..1335
                     /gene="lysA"
                     /locus_tag="IV236_001823"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8772010.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1322..2122
                     /gene="dapF"
                     /locus_tag="IV236_001824"
     CDS             1322..2122
                     /gene="dapF"
                     /locus_tag="IV236_001824"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8772011.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2125..3006
                     /locus_tag="IV236_001825"
     CDS             2125..3006
                     /locus_tag="IV236_001825"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8772012.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3003..3719
                     /locus_tag="IV236_001826"
     CDS             3003..3719
                     /locus_tag="IV236_001826"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8772013.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3725..4420
                     /gene="dapD"
                     /locus_tag="IV236_001827"
     CDS             3725..4420
                     /gene="dapD"
                     /locus_tag="IV236_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8772014.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4436..5437
                     /locus_tag="IV236_001828"
     CDS             4436..5437
                     /locus_tag="IV236_001828"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8772015.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5434..6795
                     /locus_tag="IV236_001829"
     CDS             5434..6795
                     /locus_tag="IV236_001829"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8772016.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6755..7906
                     /locus_tag="IV236_001830"
     CDS             6755..7906
                     /locus_tag="IV236_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8772017.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7881..9050
                     /locus_tag="IV236_001831"
     CDS             7881..9050
                     /locus_tag="IV236_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8772018.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9225..10604
                     /locus_tag="IV236_001832"
     CDS             9225..10604
                     /locus_tag="IV236_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8772019.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10885..11114
                     /locus_tag="IV236_001833"
                     /pseudo
     CDS             10885..11114
                     /locus_tag="IV236_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3697 a         1946 c         2494 g         2977 t
ORIGIN      
        1 ttccaataaa attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa
       61 aagaggaatg aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca
      121 gaaaagtata agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg
      181 tcattcaagc gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa
      241 gcattccaaa cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt
      301 gtcagtggtg gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac
      361 ttccatggta acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg
      421 catattgttg ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat
      481 ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa
      541 tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc
      601 caacaagccc ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct
      661 catatcgggt cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg
      721 aattacttaa ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt
      781 cgttatacac aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa
      841 tatacggaag aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg
      901 ggtagaagta ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag
      961 acaccacata aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct
     1021 ctgtatcagg cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag
     1081 aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat
     1141 ttgccgaaag cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac
     1201 tcgatgagca gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc
     1261 caatcaagac tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc
     1321 tatgatcctt cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga
     1381 agacacagat tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatacaga
     1441 cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag
     1501 ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa
     1561 catgatgaat gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt
     1621 gttggatccc tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga
     1681 aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga
     1741 aaaagtaacg atcactagtt tatttttagg aacgattcat acagttgttt ttgttgaaaa
     1801 tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa
     1861 agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac
     1921 ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt
     1981 ggttgctaag gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg
     2041 agacttgatg atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg
     2101 gacaagaata ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg
     2161 atgaatgaag agggatcagt agattatgct ggtttggaaa aattgatcca attccattta
     2221 gatgaacaaa cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca
     2281 caatctgagg aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg
     2341 gtgattgcag gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt
     2401 gcttctttgg gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat
     2461 gcaggactag ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg
     2521 tataatgttc cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca
     2581 gaacatccta atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat
     2641 gccgctcggt tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta
     2701 ccagtaatga gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa
     2761 atagtccatg aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag
     2821 caattaaatg cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc
     2881 aaagcagcca tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct
     2941 ctttcaaaag agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa
     3001 aaatgaaaat cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac
     3061 aagaaagagg agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg
     3121 cttttacgga actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa
     3181 tcctttcgta tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac
     3241 aatcacagtt ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta
     3301 atttttcgtt cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc
     3361 aaggctggca gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg
     3421 gtactgcaaa tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca
     3481 actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt
     3541 ctttacctgg tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac
     3601 atgaatcttt ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga
     3661 aagatcagtc accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa
     3721 aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt
     3781 gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga
     3841 aggaagcagc ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga
     3901 atcagaaaaa atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt
     3961 gctagatatt aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt
     4021 gtcaatcggc aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat
     4081 cggtgaaaac acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa
     4141 aaactgccat attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa
     4201 accggttatc gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt
     4261 ccatatcgga aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc
     4321 agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa
     4381 agaaaataca gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg
     4441 cgaaatagat acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt
     4501 gttagaagaa tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc
     4561 cggtaaagtt tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc
     4621 atttgaagga tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc
     4681 gccaatcgca cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga
     4741 ttcggatatt ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct
     4801 gattgcaaat ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga
     4861 agcctttggt atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca
     4921 aaaaggcatt gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta
     4981 taatattagc caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa
     5041 agaagaacag aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt
     5101 atcagcaacc tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca
     5161 actgcaaaaa gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat
     5221 tcaattagaa gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac
     5281 agatatggtt tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt
     5341 ctatacgact gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc
     5401 agctttaatg cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa
     5461 ttcggcggaa catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt
     5521 tcagatccca atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat
     5581 gacaaagtaa cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg
     5641 acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg
     5701 cagttaccta ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata
     5761 actgaagatt atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat
     5821 cttggttacc agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc
     5881 gatcaggaaa aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt
     5941 gttccagggt tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga
     6001 agcgacatta ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg
     6061 acggatgttt caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc
     6121 gaggaaatct cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat
     6181 gaagcagctg ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat
     6241 gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg
     6301 acaggaatag ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc
     6361 gatgaaatcg ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc
     6421 gaacatattc cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc
     6481 gataagttat ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc
     6541 gtgatcgaag acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga
     6601 ttatctggga aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca
     6661 caaggagcac aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta
     6721 gtgaagggta tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact
     6781 ccatcaaatt cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca
     6841 agtaaaaaac agcggggccg ttatccatga aatcggcgaa acaggactac tcctttattt
     6901 tgataatcag caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa
     6961 agaagaaacg aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca
     7021 tgacggacat atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga
     7081 tcttatttat aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca
     7141 aagcgttatt cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca
     7201 tctatggcct ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca
     7261 aagcagtgaa acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa
     7321 aggaatcgat agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg
     7381 gcaattaccg gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat
     7441 ccgcaatgtt ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga
     7501 tatgcaaaat tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc
     7561 tactatttct tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata
     7621 tgcagcactg ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga
     7681 agattttggc atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga
     7741 tacccctgct ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt
     7801 agaatggtac aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc
     7861 aaaagaggag cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta
     7921 gaatagaagt atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga
     7981 tcaaactcac cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa
     8041 tagcagcgat tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc
     8101 gggaagcggc ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg
     8161 aagttttgac gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg
     8221 aagaaggaga caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg
     8281 aactagctgg tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc
     8341 cggaacaatt tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa
     8401 actatcctaa caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg
     8461 aggttttaaa aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt
     8521 atagtggaac gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg
     8581 ggttatccaa gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag
     8641 cccttattga cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt
     8701 cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag
     8761 agcaatatca aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag
     8821 tgacccagcc aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg
     8881 actcttggca gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa
     8941 ttgcatttgg cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa
     9001 aattacatga ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt
     9061 ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta
     9121 aatttagttc aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa
     9181 ttgtttagaa tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag
     9241 ttgtcattgt aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat
     9301 acggtgaaaa tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat
     9361 gtggtatgga gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca
     9421 gaccagaaaa ctttccgcaa tccaatgttc atattttaaa taatcacgaa gtaaaaacga
     9481 tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc agataacgaa acaaaagaat
     9541 atccttatga caaattgatt ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag
     9601 gagcagatct agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag
     9661 aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga
     9721 tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg
     9781 atagaccttt aggcacttat ttagatgctg agatgacaga tattttagaa caacacctaa
     9841 atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga agcttttact ggtaacggaa
     9901 aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc agatctagtc attcaagctg
     9961 ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt cgacttagat gaacgtggct
    10021 ggatccaaac aaatgaatac ttgcaaacga atcttccaga tgtgtatgca gtaggagatg
    10081 ctacacttgc ttattctatc ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg
    10141 cacgtagaga agcacgttat gtagtaaaac atctttttga agaagtacca aacaaaccgt
    10201 tcggcggagt cgtaggttcc tctgcattaa gtgtctttga ctatcatttt gcagcaagcg
    10261 gattgaatag ctttacagca gcaaaagcag gagtagatgt acgagtgtct tattatgaag
    10321 atacgttacg accaaaatat gtaccagaag aacaaggaaa tccaaaagtg tccatccaat
    10381 tattctcgga tccaatgacc catcaattgc taggaggagc agtattatct acctatgatg
    10441 ttacaggtca aggaaatgta ttggcactag cgatccaaaa caaactgact ttagaagaac
    10501 tagcagaagc tgacttcttc ttccagccag gctttgaccg acaatggagc atgttgaatc
    10561 tagccgctca acaagcgtta ggagaagaac catttgtaga ataagaaaac gaaatatttg
    10621 aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa gaacagccaa gtaaaaacag
    10681 cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
    10741 ttattttcaa aaaagggact gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa
    10801 aaaatctaac ttttggaggt cggtattttt tatggttaaa tatacttttg aacttaaatt
    10861 aaagatcgtt catgattatt tggatggcaa aggcgattgg accgtaaaaa tccagatcaa
    10921 gagattgaag cggaaatgct taaaattcgg gagaagcaca aggattatgg ctgtttacgg
    10981 atgaccaatg aactaagaaa ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg
    11041 atcaagaagc taggaataaa agtaacggca tacacgcgta aatcacgtcg ctacaattct
    11101 tatcgaggaa aagt
//
LOCUS       DACDID010000088        10817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDID010000088 DACDID010000000
VERSION     DACDID010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.088"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV236_001834"
     CDS             complement(25..144)
                     /locus_tag="IV236_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8772020.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV236_001835"
     CDS             complement(159..1343)
                     /locus_tag="IV236_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8772021.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV236_001836"
     CDS             complement(1599..1853)
                     /locus_tag="IV236_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772022.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV236_001837"
     CDS             complement(1878..3020)
                     /locus_tag="IV236_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8772023.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV236_001838"
     CDS             complement(3080..4432)
                     /locus_tag="IV236_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8772024.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV236_001839"
     CDS             complement(4503..4847)
                     /locus_tag="IV236_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8772025.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV236_001840"
     CDS             complement(4857..5231)
                     /locus_tag="IV236_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772026.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV236_001841"
     CDS             complement(5244..5558)
                     /locus_tag="IV236_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772027.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV236_001842"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV236_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IV236_001843"
     CDS             complement(8976..9638)
                     /locus_tag="IV236_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772028.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IV236_001844"
     CDS             10255..10485
                     /locus_tag="IV236_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772029.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="IV236_001845"
     CDS             10491..10790
                     /locus_tag="IV236_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772030.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DACDID010000089        10593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDID010000089 DACDID010000000
VERSION     DACDID010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.089"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..431
                     /gene="rpsN"
                     /locus_tag="IV236_001846"
     CDS             162..431
                     /gene="rpsN"
                     /locus_tag="IV236_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8772031.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            446..625
                     /gene="rpmF"
                     /locus_tag="IV236_001847"
     CDS             446..625
                     /gene="rpmF"
                     /locus_tag="IV236_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8772032.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            657..1034
                     /locus_tag="IV236_001848"
     CDS             657..1034
                     /locus_tag="IV236_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772033.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1055..1204
                     /gene="rpmG"
                     /locus_tag="IV236_001849"
     CDS             1055..1204
                     /gene="rpmG"
                     /locus_tag="IV236_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8772034.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1228..2211
                     /locus_tag="IV236_001850"
     CDS             1228..2211
                     /locus_tag="IV236_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8772035.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2241..2363
                     /locus_tag="IV236_001851"
     CDS             2241..2363
                     /locus_tag="IV236_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8772036.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2381..3904
                     /locus_tag="IV236_001852"
     CDS             2381..3904
                     /locus_tag="IV236_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8772037.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3928..4167
                     /locus_tag="IV236_001853"
     CDS             3928..4167
                     /locus_tag="IV236_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772038.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4360..4821)
                     /locus_tag="IV236_001854"
     CDS             complement(4360..4821)
                     /locus_tag="IV236_001854"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8772039.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5016..5234)
                     /locus_tag="IV236_001855"
     CDS             complement(5016..5234)
                     /locus_tag="IV236_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772040.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5352..6968
                     /locus_tag="IV236_001856"
     CDS             5352..6968
                     /locus_tag="IV236_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8772041.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7209..8549)
                     /locus_tag="IV236_001857"
                     /pseudo
     CDS             complement(7209..8549)
                     /locus_tag="IV236_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8825..8980)
                     /locus_tag="IV236_001858"
     CDS             complement(8825..8980)
                     /locus_tag="IV236_001858"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8772042.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9276..9608)
                     /locus_tag="IV236_001859"
     CDS             complement(9276..9608)
                     /locus_tag="IV236_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8772043.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10122..10313)
                     /locus_tag="IV236_001860"
     CDS             complement(10122..10313)
                     /locus_tag="IV236_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772044.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10407..>10593)
                     /locus_tag="IV236_001861"
     CDS             complement(10407..>10593)
                     /locus_tag="IV236_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8772045.1"
                     /translation="VEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQG
                     ATNLKHRLYKKIQKMLV"
BASE COUNT         3516 a         1688 c         2062 g         3327 t
ORIGIN      
        1 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
       61 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
      121 tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
      181 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
      241 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
      301 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
      361 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
      421 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
      481 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
      541 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
      601 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
      661 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
      721 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
      781 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
      841 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
      901 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
      961 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
     1021 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
     1081 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
     1141 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa
     1201 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
     1261 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
     1321 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
     1381 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
     1441 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
     1501 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
     1561 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
     1621 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
     1681 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
     1741 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
     1801 acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa
     1861 agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat
     1921 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttactccaa
     1981 tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
     2041 gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt
     2101 caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat
     2161 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
     2221 gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
     2281 aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa
     2341 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
     2401 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
     2461 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
     2521 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
     2581 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
     2641 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
     2701 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
     2761 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
     2821 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
     2881 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
     2941 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
     3001 gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc
     3061 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
     3121 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa
     3181 aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac
     3241 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
     3301 gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
     3361 tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag
     3421 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
     3481 agttttcgat tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta
     3541 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
     3601 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
     3661 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
     3721 tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca
     3781 tttccatatt ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg
     3841 gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca
     3901 ttaaaattca atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
     3961 aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt
     4021 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
     4081 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
     4141 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
     4201 tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat
     4261 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
     4321 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
     4381 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
     4441 atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa
     4501 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
     4561 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
     4621 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc
     4681 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc
     4741 atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct
     4801 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
     4861 tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc
     4921 catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa
     4981 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
     5041 ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt
     5101 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
     5161 atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga
     5221 cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
     5281 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
     5341 agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa
     5401 aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg
     5461 taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa
     5521 gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag
     5581 atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg
     5641 aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc
     5701 ctttagacat tgatttgacc caacgattag agattggtgc cattattata gtagtggatg
     5761 taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag
     5821 gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt
     5881 gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta
     5941 acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag
     6001 tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag
     6061 tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat
     6121 atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc
     6181 atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg
     6241 cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat
     6301 cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa
     6361 atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt
     6421 ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg
     6481 attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata
     6541 tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg
     6601 tcataattat tggcattttg attattacct cttttattcc cttatttgta ttctctatag
     6661 aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca
     6721 aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg
     6781 cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata
     6841 atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta
     6901 tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca
     6961 tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata
     7021 atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca
     7081 acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac
     7141 ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac
     7201 tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg
     7261 gtagtattct tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag
     7321 ttttgaaagt ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa
     7381 cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat
     7441 ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt
     7501 aggagcgcga cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag
     7561 acttcgtagc ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct
     7621 tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac
     7681 gcattctttt gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt
     7741 ttaaaggtct tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta
     7801 atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga
     7861 tagggtgtat agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag
     7921 cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga
     7981 tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg
     8041 aattcatcga aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga
     8101 aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa
     8161 gacatagaaa gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg
     8221 gagcagcgac gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat
     8281 ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag
     8341 atggatactt ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga
     8401 aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg
     8461 attcgctctt tgcgaaaggc atcttcatga aagataagat ttaggtctag aatatctagt
     8521 aactctttgg taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg
     8581 actttattct aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat
     8641 tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa
     8701 attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta
     8761 aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta
     8821 attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag
     8881 tttggctcac tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc
     8941 gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta
     9001 atggacggac gacaatctct tttttcatgt gtgaatctac catatagtaa cgtagaatac
     9061 gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag
     9121 taatatccat cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt
     9181 ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc
     9241 tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat
     9301 tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta
     9361 gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct
     9421 ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct
     9481 gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta
     9541 gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt
     9601 tttctcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat
     9661 agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt
     9721 taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag
     9781 ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga
     9841 agtcctcgtt gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt
     9901 aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat
     9961 taaaaaacgt tgataattaa aatcattcgt tttagattct atgaatcatt atagaacgct
    10021 gtaaaatcga tactttataa ttataaagaa gatagtttca aatttattgt tcgaaatttg
    10081 aaactatctt tttacttatg tcacagactc ccttagttga gttattcttt aatttcgaat
    10141 ttcgagatag ctagatatat tgcaagcata cattcttgat aacaatcttg atcatatttt
    10201 ccagttctcc aggattgctt atgaatcaaa gagctaaatc ttttgagaat tgcctcaaca
    10261 gcttcttcat ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt cataaatcta
    10321 ccttcttccc atggacttat aatctatttc tttgggaagg aggagaacga gcaaagaagc
    10381 atacaaaatt agttattatg cagatattaa accagcattt tttgaatttt tttgtaaagt
    10441 cgatgtttta aatttgtcgc accctgtctt gtaatcccca gaacctttcc aatttcttta
    10501 tcagttttat caaagataaa tttttcgata agaaaaattt gttcaggttc tttcaacaaa
    10561 ggaaggattt ttgctaattt tatatcttca aca
//
LOCUS       DACDID010000090        10588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDID010000090 DACDID010000000
VERSION     DACDID010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.090"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            163..2223
                     /locus_tag="IV236_001862"
     CDS             163..2223
                     /locus_tag="IV236_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772046.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2306..2875
                     /locus_tag="IV236_001863"
     CDS             2306..2875
                     /locus_tag="IV236_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772047.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3050..3856)
                     /locus_tag="IV236_001864"
     CDS             complement(3050..3856)
                     /locus_tag="IV236_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772048.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3994..5508
                     /locus_tag="IV236_001865"
     CDS             3994..5508
                     /locus_tag="IV236_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8772049.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5911..6894)
                     /locus_tag="IV236_001866"
     CDS             complement(5911..6894)
                     /locus_tag="IV236_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8772050.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6899..7546)
                     /locus_tag="IV236_001867"
     CDS             complement(6899..7546)
                     /locus_tag="IV236_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8772051.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7631..8263)
                     /locus_tag="IV236_001868"
     CDS             complement(7631..8263)
                     /locus_tag="IV236_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8772052.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="IV236_001869"
     CDS             complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="IV236_001869"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8772053.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8827..9564)
                     /locus_tag="IV236_001870"
     CDS             complement(8827..9564)
                     /locus_tag="IV236_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8772054.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9561..10550)
                     /locus_tag="IV236_001871"
     CDS             complement(9561..10550)
                     /locus_tag="IV236_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8772055.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3418 a         1998 c         2109 g         3063 t
ORIGIN      
        1 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
       61 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      121 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      181 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      241 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      301 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      361 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      421 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      481 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      541 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      601 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      661 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      721 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      781 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
      841 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
      901 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
      961 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1021 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1081 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1141 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1201 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1261 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1321 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1381 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1441 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1501 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1561 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1621 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1681 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1741 caaaaagaac aaaaaagacg agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1801 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     1861 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     1921 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     1981 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2041 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2101 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2161 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2221 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2281 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2341 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2401 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2461 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2521 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2581 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2641 gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2701 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2761 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     2821 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     2881 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     2941 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3001 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3061 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3121 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3181 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3241 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3301 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3361 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3421 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3481 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3541 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     3601 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     3661 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     3721 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     3781 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     3841 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     3901 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     3961 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     4021 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     4081 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     4141 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     4201 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     4261 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     4321 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     4381 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     4441 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     4501 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     4561 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     4621 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     4681 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     4741 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     4801 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     4861 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     4921 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     4981 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     5041 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     5101 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     5161 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     5221 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     5281 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     5341 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     5401 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     5461 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     5521 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     5581 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     5641 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     5701 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
     5761 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
     5821 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
     5881 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
     5941 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
     6001 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
     6061 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
     6121 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
     6181 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
     6241 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
     6301 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
     6361 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
     6421 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
     6481 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
     6541 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
     6601 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     6661 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     6721 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     6781 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     6841 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     6901 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     6961 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     7021 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     7081 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     7141 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     7201 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     7261 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     7321 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     7381 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     7441 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     7501 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     7561 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     7621 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     7681 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     7741 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     7801 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     7861 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     7921 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     7981 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     8041 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     8101 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
     8161 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
     8221 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
     8281 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
     8341 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
     8401 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
     8461 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
     8521 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
     8581 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
     8641 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
     8701 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
     8761 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
     8821 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
     8881 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
     8941 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
     9001 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
     9061 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
     9121 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
     9181 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
     9241 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
     9301 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
     9361 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
     9421 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
     9481 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
     9541 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
     9601 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
     9661 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
     9721 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
     9781 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
     9841 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
     9901 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
     9961 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
    10021 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
    10081 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
    10141 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
    10201 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
    10261 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
    10321 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
    10381 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
    10441 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
    10501 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
    10561 ctccttttaa aattttcttt tatcatac
//
LOCUS       DACDID010000091        10459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDID010000091 DACDID010000000
VERSION     DACDID010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.091"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IV236_001872"
     CDS             complement(247..759)
                     /locus_tag="IV236_001872"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8772056.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV236_001873"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV236_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8772057.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IV236_001874"
     CDS             complement(3212..3646)
                     /locus_tag="IV236_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8772058.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IV236_001875"
     CDS             complement(3656..4444)
                     /locus_tag="IV236_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8772059.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IV236_001876"
     CDS             complement(4441..5382)
                     /locus_tag="IV236_001876"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8772060.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IV236_001877"
     CDS             complement(5559..6146)
                     /locus_tag="IV236_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8772061.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV236_001878"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV236_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8772062.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IV236_001879"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IV236_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8772063.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IV236_001880"
     CDS             complement(9063..9998)
                     /locus_tag="IV236_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8772064.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10184..>10459
                     /locus_tag="IV236_001881"
     CDS             10184..>10459
                     /locus_tag="IV236_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772065.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         2927 a         2360 c         1813 g         3359 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    10141 aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct
    10201 taccatagac gaactgacaa tgatagaatc atattatctt caacataata agccggttga
    10261 aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa
    10321 gcaaggcaag actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg
    10381 tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga aagtcactct
    10441 tggttggacg cctgacgtc
//
LOCUS       DACDID010000092        10403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDID010000092 DACDID010000000
VERSION     DACDID010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.092"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..1260
                     /gene="mltG"
                     /locus_tag="IV236_001882"
     CDS             85..1260
                     /gene="mltG"
                     /locus_tag="IV236_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8772066.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1396..1875
                     /gene="greA"
                     /locus_tag="IV236_001883"
     CDS             1396..1875
                     /gene="greA"
                     /locus_tag="IV236_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8772067.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2044..2775
                     /locus_tag="IV236_001884"
     CDS             2044..2775
                     /locus_tag="IV236_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8772068.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2772..3839
                     /locus_tag="IV236_001885"
     CDS             2772..3839
                     /locus_tag="IV236_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8772069.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3846..4478
                     /locus_tag="IV236_001886"
     CDS             3846..4478
                     /locus_tag="IV236_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772070.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4618..5283
                     /locus_tag="IV236_001887"
     CDS             4618..5283
                     /locus_tag="IV236_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8772071.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5311..5604
                     /locus_tag="IV236_001888"
     CDS             5311..5604
                     /locus_tag="IV236_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8772072.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5672..6619)
                     /locus_tag="IV236_001889"
     CDS             complement(5672..6619)
                     /locus_tag="IV236_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8772073.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6797..7600)
                     /locus_tag="IV236_001890"
     CDS             complement(6797..7600)
                     /locus_tag="IV236_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8772074.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7746..8660
                     /locus_tag="IV236_001891"
     CDS             7746..8660
                     /locus_tag="IV236_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8772075.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8708..9178
                     /locus_tag="IV236_001892"
     CDS             8708..9178
                     /locus_tag="IV236_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8772076.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9263..9871
                     /locus_tag="IV236_001893"
     CDS             9263..9871
                     /locus_tag="IV236_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8772077.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            10005..10205
                     /locus_tag="IV236_001894"
     CDS             10005..10205
                     /locus_tag="IV236_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8772078.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3542 a         1594 c         2131 g         3136 t
ORIGIN      
        1 atatttaaat aaaatcattc acgccaattc aaatatcatg tataatgaat aaagttacga
       61 acttgatagt aaggaggagt tgttttgtca ggaaacaaac aacacccaag taaagataaa
      121 aaagaagcca gtaaggcagc tacagcagat ataatcgaca agcggagaaa tctgcgtaaa
      181 aaagaagata agattgtgag gaagatcatt ttagtgattg ctcttacatt attgattatt
      241 ggcggatttc ttggatttac cgtgtatcgt tatgtagata gcgggttgaa gcctttagat
      301 aaatcagatg accaattagt ccaagtcgag atcccgagtg gctcttctaa caagcagatt
      361 ggtgagattt tggagaagga caatattatt aaaagcggga ttgttttcaa ttattataca
      421 aaatttaaaa atttaactgg ctttcaagct ggctattatc agttagcgcc taatatgact
      481 cttgacgaga ttggcaaaca gttgcaagaa ggcggtactt ctgagccaac aaaagttgca
      541 gatgggaaaa ttgccattcc agaagggtac gatatcgatc agattgctga acgggtagcg
      601 aaggtaactg gtaaagacaa gaaagaattt ttagatttag tgaatgatga gactttcttt
      661 aacgagttgc accaaaaatt cccagaactg ttggaaagtg catctaaagc tgagaacaca
      721 aaataccgtt tggaaggtta cttattccca gccacatatg attattacac gagtacttcg
      781 cttaaggatc tagtgattga aatggtgaac aagacgaata cggtcatgca aaactattat
      841 tcagctatca agcagaaaaa tttgactgta caagaagttt taactctagc ttctctagtt
      901 gaaaaagaag gcgtaaaaga aaatgatcgt aaaaacattg cacaagtctt ctttaaccga
      961 atcaaagcaa atatgccact tcagtcagat atctctgttt tatacgcatt aggtgaacac
     1021 aaagaattag tgacgtatga agatacagct gtagattcac catataattt gtataccaat
     1081 accggctatg gacctggacc ttttgacaat ccaagcgaag aggcaatcaa ggcagtatta
     1141 gaaccagcag aaaatgacta ttattatttc gtagcagata cgagcactgg aaatgtttat
     1201 ttcgctaaaa cttatgaaga acatatggaa ttagtggaaa aatatgtaaa taattcttag
     1261 aaaaacaggc tctagacatt gatcattttt agaaaaacat ttacatttga agaaaagcgt
     1321 ggtaatcttt tttggactat attcctcaaa agattacctt ttgcggtatt ttatcgaaaa
     1381 aaggagacgt tacacatggt agagaaagtt tatccaatga ctctagaagg taaagaaaaa
     1441 ttagaaaaag aattagaaga actaaaaaca gtcaaacgcg gagaaatcat tgaacgaatc
     1501 aaaattgccc gcggtttcgg agatctttcc gaaaactctg aatatgaatc agcgaaagat
     1561 gaacaagcat ttgttgaagg aagaatcaca acaattgaaa atatgattcg ttttgctcaa
     1621 atcatcgata acgacggtgt agatgcagat gaggtatcaa ttggtaaaac agttacattc
     1681 atcgaactac cagatggaga agaagaagag tacacgattg tcggaagcgc tgaagcagat
     1741 ccgttttctg gcaaaatttc caatgattct cctattgcgc aagcattgat tggcaaacgt
     1801 aaaaatgatg aagtaactat tgctactcca ggtggcgata tgttagttaa aatcgtcaaa
     1861 gtagaacaag cgtagtctga ctgaaagatc tagagagttc atcttactga tgatacactc
     1921 tagatctttt tctctttgtg tattttagat ttcaacaaac gatcttacat ctaaagtcct
     1981 atagccaaca gttaaaaaaa taggtatgat gaaatgagaa tgtgtcagga gggagaaaga
     2041 gcgatgaata gttcttggcg tttttttgtg gtagttgaag cactactgct gttatttgct
     2101 gtttggcaaa tcgtaaacaa tactggactt ttgcttttag tgatattcgg tattttcaat
     2161 atatatctag ctatgcgcaa acatcctcgt actaaatttc aaaattttca attgatatta
     2221 ggaagtttgg tcattttttt tagcttagtc aacagtcctg ctttatggat gatggctgta
     2281 tttgccgttt tatttattgg tctgaaaggt gtagagattt cagggataga tttaaccaaa
     2341 aatgcttttt ggcgaaagaa acagatcatg atgatccaaa cagagcaatt aaaaacacat
     2401 aataatgagc ggaaaagaca gcaattattc ggtaaccaac gtattggaaa cgacgtctat
     2461 gaatgggacg atatcaacat cgccgtcatt tctggtgata ctatcataga tttgggcaat
     2521 acgcttttgc caaaagatga taatattgtg attgtaagaa aagggatagg cagaacacgg
     2581 atcttagtac ctttgggcgt agccatccga ttagagcatg cgacattagt aggaaatgtc
     2641 ttgtttgaag aagagcaatt ttctttgaaa aatgaacaaa tcaaaatcta cagcagtgat
     2701 tatgatgaaa accctcgtag attgaagatt attactaata ccttgattgg agatgtcgag
     2761 gtgatacgaa tatgatggga aaaatatcca gagcgatgct agctgtttat tcggggattg
     2821 cggctttcct tattattcta ttttcacttt tcacttattt ttatgccagc aatcaaagtc
     2881 attggtgggg agaattactg cgtgcacgtt tattatatgt tccgcttatc ttccatttgt
     2941 tagccatttc cttaggtgta ggattaattg tctttctatt attatcactt attcaaaaga
     3001 caaaatacgg gaaaatcgaa gaaaagctgc gtgcactttc ttctggcaat tatgaatcca
     3061 aattgttgct tcttccgatc ccaagtgcat cggacgattt atacatcaaa gatattgata
     3121 aggaaatcac taagataaaa gaaaaaatga ttgaagtatc tagtgaatta caaattgtaa
     3181 cgagccgtcc ccaatatgta gatggacaaa ccaaagaaga aattttagaa ttagaaagac
     3241 atcgattagc ccgtgagctg catgattcag tttcgcaaca attgtttgca gcaatgatga
     3301 tgatgtcggc attgacggag caagcagaaa aaagcgagac accagaaatg tttcgcaagc
     3361 agttgaaaat ggtagcagag atcatcaacg cttcccagtc tgagatgcgt gcgctgcttc
     3421 ttcatctacg tccagtcaat ttagaagaga aaagtttaaa gcaaggtatc gagcagttat
     3481 tgaaggaatt gcaaaataaa atccagattt cgctgaaatg ggatgtagaa gatgtaaaac
     3541 tatctagttc cattgaggat cacttgttcc gaatcgttca agaattgtta tcaaatacat
     3601 taagacatgc taaagccaat gaattagagg tatatttgca caaaatagac aataatcttt
     3661 tgttacgtat tattgacgac ggaacagggt ttaatatgaa tgaaacgaaa acgggaagtt
     3721 atggattgaa caatatcaaa gaacgagtag ctggtattgg tggaacagta aaaatcatta
     3781 gttttaaagg tcaaggtaca agtgtagaaa tcaaggtccc tttgatgaag gaggcataga
     3841 taaggatgat aaaagtttta ttagtagatg accatgaaat ggtgcgctta ggcgtctctt
     3901 cttatttatc tattcaagaa gacattgaag taattggaga agctgaaaat ggacgacaag
     3961 gctatgagaa ggcgatgaca cttcgaccgg acgtcatttt aatggactta gtcatggaag
     4021 aaatggatgg tatcgaatca acaaaagcaa tcttaaaaga ttggccagaa gctaaaatca
     4081 ttatcgtaac cagttttatt gatgacgaaa aagtttatcc tgccattgaa gcaggtgcag
     4141 cagggtattt gttgaagacc tctaccgcac atgaaattgc tgatgctata agggcaacgc
     4201 aacgtggcga gcgagtatta gaaccagaag tgacaacaaa aatgatggaa aaaatgagtc
     4261 ggcgaaatga gcctgtatta catgaagaac taacgaatcg ggaaaatgaa attttaatgc
     4321 tgatttctga aggaaaaagt aatcaggaaa tagcggacga attgtttatt acactgaaaa
     4381 cagtaaaaac acatgtttcc aatatattgg ctaagctaga agtggaagac cgtacacaag
     4441 ccgctatcta tgcattcaaa catggtttgg tgaaataaaa acgatgataa agaggataat
     4501 caaaagctga gaaaataact attatttttc ttggcttttt tacaataatg atgcttgctt
     4561 ttgtttttgt aaaggctttg ttatactgag aaaagaagat actaaaggag gaaaacggtg
     4621 aaacaaaatt ttgcaattgt aggtcttgga cgatttggcg gaagtatctg ccgaacactg
     4681 atcgaagctg gtcaagaagt actggctatt gatagtaatg aagatcgggt caatgaatat
     4741 atgaatatag ccactcatgc tgttgtggca aatgcacagg atgaaatgac acttcgatca
     4801 ttaggcataa gaaatttcga tcatgtaatt gtcgctattg gagaagatat tcaagccagt
     4861 atcttagtta ctctgatggt aaaggaaatg ggcgtaccaa atattttagc aaaagcccaa
     4921 aatgagtatc atgccagagt acttgaaaag atcggagctg atcgcgtcgt ccatcccgag
     4981 cgagacatgg gaataaggat tgcgcataat ttagtgtcaa agaatatttt agattaccta
     5041 gagttgtcag ataaatattc tttagcagaa atccgagtct ccaatcctaa atttttcaac
     5101 aaaacattag ctgaattaaa tttcaggcaa cgttttgact taacagtagt cgccatacga
     5161 agagtagatc aaacggttat cgcttctcct gcagcagatg aatatgttcg tgaaaatgat
     5221 aatctactgg ttattggaga gacaggcgat gtggatattc tagacgataa gatgaatcaa
     5281 tagaaagaaa agagaaagta ggttgttggg atgaaattaa cggtaacgcc aaaagcacaa
     5341 aagtggtttg actcagaaat tgatctgcca gaaacttacg gaatacgttt ttttggaaaa
     5401 gtctacggca aaacagaggt tcatgatggt ttttcgattg gaatgtcagt tgaacagcca
     5461 gaacatcctg taaagaaagt ggaaatggat gggacacttt tttttatcga ggaagcagat
     5521 gaatggttct ttaaaggata tgatttactt gttgattatg atcccaacct ggaggagcca
     5581 acctatcact ttaaaaaaca ataaaaaaag agagggtgac acaatttttg tcaccttctc
     5641 ttttttcttt tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat
     5701 tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta
     5761 atttcttttg aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca
     5821 ttttccaaag aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa
     5881 aggaaagttg ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac
     5941 ggccaacatt gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga
     6001 gcccagttgt ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa
     6061 cttcctttag ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg
     6121 tattgattcc atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa
     6181 tatggccagt tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg
     6241 aacctaacca tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc
     6301 caaggatgat tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg
     6361 aaagtaaaat atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga
     6421 tagattggac ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc
     6481 agcccaaact gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat
     6541 aaccaggcgc tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat
     6601 aaatgattct ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata
     6661 caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa
     6721 aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac
     6781 gagttgtctt tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa
     6841 aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca
     6901 tcaagattgt tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat
     6961 caagaaaagt agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa
     7021 atagcaaagt gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga
     7081 cttttatcca aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta
     7141 agttttttaa agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag
     7201 aagtcgtgcc tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa
     7261 caaaattccc catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa
     7321 atgtgaagat aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct
     7381 gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa
     7441 tgttgtttaa gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa
     7501 ctaaaagata gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga
     7561 atcgtataaa ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag
     7621 tgtcatcact tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag
     7681 ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa
     7741 ataaaatgga ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg
     7801 gagtagtggt tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag
     7861 ggagtttgtc tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt
     7921 tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct
     7981 accagcgttt agaaatattg agtgctttca tcaactcatt catattgatc atattatcct
     8041 tgtttttaac cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt
     8101 tgatgctaac agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac
     8161 gacaagaagc agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata
     8221 cactgtcttc ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact
     8281 ggatcgatcc agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg
     8341 tgatcaaaaa agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa
     8401 tggaacaaga aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc
     8461 agtatagcga aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa
     8521 gcgagattga aaagatatat cctcgtataa tttctttatt aaaattaaaa tatggtatta
     8581 cacatgtgac gatccaagca gagacaaagg caaaggatca aaaaaaatta atttttttaa
     8641 ataaagatga aagttattga aatatagagt atgataaata agaattttta ataaggtagg
     8701 ttggctcatg aagcagtatc gacagttaaa aggatattta gaagcaaaag gatattggga
     8761 gatactagtc tatctctttt ttggtggttt ggctacgatt gtaaattttg ttagttttgc
     8821 tctcgcacgc caatattttg atttaagtat ggccttatcc aattctattt cgtggttttg
     8881 ttctgtattg tttgcttttg taacgaacaa actttgggta ttccattcaa aatcaccgaa
     8941 ttttacacat gcacttattg aatgcggtaa gttcttcttc taccgtatcc tttcttacgg
     9001 attggacatg ggggcaatgg tattgttgat caatgttatg aacagcaatg agtatgtggc
     9061 gaaaatcatc actcaaatta ttgttatcct tgcgaactat attttcagca agttgtttat
     9121 ttttaaagaa actgaggttt ttgaagaaga agtaggtcaa agtgaaaaag acgactaggc
     9181 ataacaattg aagaatttcc ttctctttgt tactctttgt aatgtagaca ttattaagaa
     9241 aacattgagg aggaattttt ttatgactta cacattacca gatctacctt atgcatatga
     9301 cgcattggaa ccttatattg acgaagaaac gatgcatctg catcatgata agcatcacaa
     9361 tacttatgtg acgaatttaa attcagcaat tgagaaatac ccagaattag gcgaaaaaac
     9421 aatagaagaa ttattatctg atatggacgc tattccaaca gatatcaaga cagctgtacg
     9481 taacaatggt ggcggacatg ctaaccattc atttttctgg gaaatcatgg caccaaatgc
     9541 tggtggcgaa cctacaggag aaataaaaga agcgattaat gaagcttttg gtgatttttc
     9601 ttcttttaaa gaagaattca aaaaagcagc cgctggacga tttggttctg gatgggcttg
     9661 gcttgtaatg gaaaatggaa aattagctat tacctctact gcaaatcaag attctccatt
     9721 gatggaagga aagacaccaa ttctaggttt ggacgtttgg gaacatgctt attatttgaa
     9781 atataaaaat gtccgaccag attatattgc agcattctgg aatgttatta attgggatga
     9841 agtaaacaaa cgtttcgaag cagcaaaata accatttcct ccgtcatatc agtcttaaaa
     9901 ataatttgga aaataaaaga gcgaaagctt gctagcgttt tctaaattgg gtataatgtt
     9961 cttgcaaggt tacttgcaag aacttttttg gaggatatac atttatggaa cacggaacgg
    10021 ttaaatggtt caacaatgaa aaaggatttg ggtttattac agtagacggc ggagatgacg
    10081 tatttgttca tttctcagca attcaaggag acggcttcaa gagcctagaa gaaggtcaag
    10141 aagtagaatt cacgattgtt gaaggtgcac gcggacctca agctgctgaa gtgactaaat
    10201 tataataatt tgttgaataa agacacgact tcttagagag tcgtgtcttt ttgtctattt
    10261 atgaagtttt tagatttttt ggctaagtaa atgcatttgt tcacttgaca tgtcctaact
    10321 aattatcaag caaggaacat tttcttcgtt agtaatggat tttaaagaaa tttcagtttt
    10381 tggatgttga ataaaagacc att
//
LOCUS       DACDID010000093        10245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDID010000093 DACDID010000000
VERSION     DACDID010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.093"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..236
                     /locus_tag="IV236_001895"
     CDS             87..236
                     /locus_tag="IV236_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8772079.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            247..1761
                     /gene="dltA"
                     /locus_tag="IV236_001896"
     CDS             247..1761
                     /gene="dltA"
                     /locus_tag="IV236_001896"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8772080.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1758..2963
                     /gene="dltB"
                     /locus_tag="IV236_001897"
     CDS             1758..2963
                     /gene="dltB"
                     /locus_tag="IV236_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8772081.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3008..3241
                     /gene="dltC"
                     /locus_tag="IV236_001898"
     CDS             3008..3241
                     /gene="dltC"
                     /locus_tag="IV236_001898"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8772082.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3244..4512
                     /gene="dltD"
                     /locus_tag="IV236_001899"
     CDS             3244..4512
                     /gene="dltD"
                     /locus_tag="IV236_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8772083.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4653..5735
                     /locus_tag="IV236_001900"
     CDS             4653..5735
                     /locus_tag="IV236_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8772084.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5735..6538
                     /locus_tag="IV236_001901"
     CDS             5735..6538
                     /locus_tag="IV236_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8772085.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6608..7348
                     /locus_tag="IV236_001902"
     CDS             6608..7348
                     /locus_tag="IV236_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8772086.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7359..8363
                     /locus_tag="IV236_001903"
     CDS             7359..8363
                     /locus_tag="IV236_001903"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8772087.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8408..10171)
                     /locus_tag="IV236_001904"
     CDS             complement(8408..10171)
                     /locus_tag="IV236_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772088.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3289 a         1697 c         2072 g         3187 t
ORIGIN      
        1 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
       61 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
      121 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
      181 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
      241 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
      301 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
      361 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
      421 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
      481 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
      541 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
      601 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
      661 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
      721 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
      781 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
      841 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
      901 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
      961 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
     1021 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
     1081 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
     1141 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
     1201 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
     1261 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
     1321 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
     1381 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
     1441 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
     1501 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
     1561 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
     1621 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
     1681 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
     1741 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
     1801 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
     1861 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
     1921 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
     1981 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
     2041 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
     2101 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
     2161 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
     2221 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
     2281 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
     2341 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
     2401 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
     2461 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
     2521 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
     2581 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
     2641 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
     2701 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
     2761 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
     2821 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
     2881 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
     2941 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
     3001 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
     3061 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
     3121 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
     3181 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
     3241 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
     3301 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
     3361 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
     3421 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
     3481 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
     3541 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
     3601 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
     3661 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
     3721 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
     3781 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
     3841 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
     3901 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
     3961 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
     4021 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
     4081 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
     4141 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
     4201 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
     4261 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
     4321 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
     4381 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
     4441 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
     4501 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
     4561 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
     4621 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
     4681 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
     4741 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
     4801 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
     4861 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
     4921 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
     4981 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
     5041 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
     5101 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
     5161 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
     5221 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
     5281 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
     5341 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
     5401 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
     5461 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
     5521 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
     5581 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     5641 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     5701 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     5761 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     5821 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     5881 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     5941 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     6001 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     6061 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     6121 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     6181 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     6241 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     6301 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     6361 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     6421 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     6481 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     6541 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     6601 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     6661 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     6721 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     6781 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
     6841 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
     6901 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
     6961 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
     7021 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
     7081 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
     7141 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
     7201 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
     7261 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
     7321 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
     7381 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
     7441 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
     7501 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
     7561 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
     7621 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
     7681 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
     7741 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
     7801 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
     7861 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
     7921 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
     7981 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
     8041 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
     8101 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
     8161 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
     8221 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
     8281 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
     8341 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
     8401 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
     8461 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
     8521 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
     8581 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
     8641 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
     8701 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
     8761 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
     8821 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
     8881 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
     8941 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
     9001 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
     9061 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
     9121 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
     9181 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
     9241 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
     9301 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
     9361 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
     9421 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
     9481 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
     9541 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
     9601 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
     9661 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
     9721 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
     9781 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
     9841 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
     9901 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
     9961 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    10021 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    10081 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    10141 agaaataagc aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta
    10201 tacgtaaata aaaagattct tttttattat atatgttatt aaaaa
//
LOCUS       DACDID010000094        10193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDID010000094 DACDID010000000
VERSION     DACDID010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.094"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..968)
                     /locus_tag="IV236_001905"
     CDS             complement(<1..968)
                     /locus_tag="IV236_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8772089.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLK"
     gene            complement(959..1627)
                     /locus_tag="IV236_001906"
     CDS             complement(959..1627)
                     /locus_tag="IV236_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772090.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2083..3069)
                     /gene="lacD"
                     /locus_tag="IV236_001907"
     CDS             complement(2083..3069)
                     /gene="lacD"
                     /locus_tag="IV236_001907"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8772091.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3082..4008)
                     /gene="lacC"
                     /locus_tag="IV236_001908"
     CDS             complement(3082..4008)
                     /gene="lacC"
                     /locus_tag="IV236_001908"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8772092.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4021..4536)
                     /gene="lacB"
                     /locus_tag="IV236_001909"
     CDS             complement(4021..4536)
                     /gene="lacB"
                     /locus_tag="IV236_001909"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8772093.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(4555..4983)
                     /gene="lacA"
                     /locus_tag="IV236_001910"
     CDS             complement(4555..4983)
                     /gene="lacA"
                     /locus_tag="IV236_001910"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8772094.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5003..5776)
                     /locus_tag="IV236_001911"
     CDS             complement(5003..5776)
                     /locus_tag="IV236_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8772095.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            5941..6420
                     /locus_tag="IV236_001912"
     CDS             5941..6420
                     /locus_tag="IV236_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8772096.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6477..6779
                     /locus_tag="IV236_001913"
     CDS             6477..6779
                     /locus_tag="IV236_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8772097.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            6801..8258
                     /locus_tag="IV236_001914"
     CDS             6801..8258
                     /locus_tag="IV236_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8772098.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(8851..9603)
                     /locus_tag="IV236_001915"
     CDS             complement(8851..9603)
                     /locus_tag="IV236_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8772099.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(9818..10054)
                     /locus_tag="IV236_001916"
                     /pseudo
     CDS             complement(9818..10054)
                     /locus_tag="IV236_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
BASE COUNT         2897 a         2234 c         1735 g         3327 t
ORIGIN      
        1 tgcttgagcc catgcactcg gtactgatga gaaaaaattg atgcctgttt ctcctgagga
       61 atgccaattc cctgatcttt tacttcaata atcacgctac ccttttggtt tgaaatggtg
      121 acaattacca gttttccttc accatatttg attgcattgt caactaaatt cgaaaccgcc
      181 cgataaatcc aacgatctcg tccatgaatc agggttggtt gatcttcagt aaacgcaaac
      241 aaaagattcg gataattcat tttgtaggca tcacaaactt ccgcacagat cagtgaaacg
      301 tctacgactt ccgtgctaag atcctctgag ttattggata atgtcaaaat atcgtcaata
      361 ctgtcggtca tccgatctaa aagcattaaa tttccttgat cggcatgttg ccgttctatt
      421 tccagattcg tacgcaaaat agcgatggca tttttctgct cgtgggtcag ataactattt
      481 aatcgtcgat aatcctccaa atgaccatcg aacttatcct ttagcttatt aaaagccgca
      541 tttaatgcct cgtccttcac agtattttta ggaacttgat caataaacgt taaatcacgc
      601 gtaatcgcta agatttgatt actgtaaatt ttgcgtaaaa ccaaccaaag aataaacgtt
      661 aataacatga cggcagaaca aataaccagc accatcgctg gtaaatattt aaatagttcg
      721 tcctggtaag ctttatctat catcgaaata gtcataaatt gattgttgga agatttcgaa
      781 gcttccatct gcgtctctgc ttgaaccgtc ttaccctctt ccataaaata aacatcttca
      841 cctaaggtag atgcgatact gattccctcc aaattatttc tcagaaagaa gaaactcact
      901 aaaatcacgc ctgtcaccaa cagtgaaaaa gccagcatcg ttaacgtcat tttaaaattt
      961 aatagcatag ctggtacccc tttgctcgaa tggtttttaa gacctctttt tcagctgctt
     1021 cagccagttt cttttttaga cgagccagat gcactcgcag aaccgacgaa aaaggatcaa
     1081 aatcttcatc gtagacatgt tcagaaattt cctcagacga aacaaccgct ggatgacgct
     1141 gagcgatgca cagtaagata tcaaattcct tgggtttcag gaccacctct gtcttttgat
     1201 agctgaccct tcgacctaat ggatcaatct tcaaagcacc aattgaaatc tgcggcagcg
     1261 cacgtccatg ataacgacga ataaccgcgc gtattcgtgc ttttaattct agtaactcaa
     1321 agggtttgac taaataatca tctgctccaa aatccaagcc ttttgctcgt tcttcaatct
     1381 catcacgtgc agtaataatg ataactggac tttcgacttc ttcagcacgg agactttgta
     1441 aaacagataa tccatctttt ttaggcagat ttaaatctag tagtatccca tcgtatgaat
     1501 tgatgaaggc cttctcttct cctgactcgc catcataagc tacatctaca gaaatattct
     1561 cctgctctag gccctttttt acggataaaa ccagctcacg attgtcctca accaccagta
     1621 cacgcatgta tattcactcc tcatacggat ttagtaatta cacttatgct ttttagcata
     1681 gtacacgctc ttcttcaaag tccacgttct ttttgtgaag atttgaagaa attcatacat
     1741 tctgttaaaa aacacataat taagaatgat tttactgttt gaaaatacct agaatagaaa
     1801 aagactggaa cctttcctcc agtctcacta ctttctattt gcttggtttt cttgtatata
     1861 cttttcaaag ttcgttggtt tatgaccaat aagcctcgtt agaatactga aaattgccat
     1921 caagtcatta agtaccacat gtaagaaaat ctctctaaag aaaaacccct tcgttttaac
     1981 tcacttacta ttttagaatt cgttccccta ataatgcagc atgattttac gctttatgta
     2041 taaaaaaaga ctgcaagcaa ttttgcttca cagtctgatt tcttatgata gtgagcttgt
     2101 taacacttcc accggtgtag cagtttcttt caatacggca tttaattctt caatattttt
     2161 acgcccttgc gtattcatcc actccatcgc tgcttctttt ccatcaacga tatagctttc
     2221 tacaccatct gcccaagtcg cacggccgca aagtacacca ttgaaggttg agccggcatt
     2281 tttcgcgaag cgcagtgttt cttggaacaa ctctgctttt acaccggcgc ttaggaaaat
     2341 aaatggcagg ttagtggctt cagcctgttc cttgaagaag ttagccgctt gtgcctttgt
     2401 gtacacgact tcgtctgtcc catagccttc cacataattc atattgacgg gtacttccac
     2461 ctttaacaca tctacgccat aacgtggatc agaaaattcc ttcatcatat cgttgacttt
     2521 atgaggtttc ttaatggcgt attctttcga agtcgcatca gagatgttcg cgtcataaga
     2581 aacaagctcc aagaagaagg gtaagttttc agcctgacat tctgagccga ttcgttcaat
     2641 aaaggcatgt ttttgattat tgatttcttc tgcttcatcg acatcatagt aaagtaggaa
     2701 cttacaagca tcggcgcctg cttcttttaa acgtttaact gaccagatcg ataataaatc
     2761 tggtaaacgg ccgggagttg acgcatcgta accagttttt tcataagcta acagcaagcc
     2821 ggcatttttt gctctaacgt cagctgctgg caagccatat tcgggatcta ataaaatcga
     2881 tgaggcatat ttcgttaatt gtgaagaaac aatttcttta aattcaacga tttctgattc
     2941 ttctgcttcg ctgcccttga attttgtgat catcttcttc aaagccccgc gttgatcaat
     3001 tgctaaggcc ccaatcactc catcttcatt gcatagttgc ttgatatatt gacgtttttc
     3061 ttcgttcatt tccgtatcct cctacacttt ctccactaag atttgatcat atagtttttg
     3121 ataattggct aattggacat agccagtcgt cgcttcctga gcattcagca ttcctaaaac
     3181 attaccctgt tttaacacgg cttcatccga aagaccttgt tgaatcgcag cggtcaatcc
     3241 ggcaacggtc gcatcaccag agccaactgg attgacgaca tcaatctttg gaattgtcac
     3301 gcaataaaaa gtctcgtgat gtttggcaaa ggctccgtcg ccccccattg aaaccactat
     3361 ccattcaatt tcatcaaaca tcgaatctga caaggcggct tttaaatctg ataattctgt
     3421 tgagatttct cgtcctagca gaccagccag ctcttcggta ttgggtttta ttagcttagg
     3481 ctttacactg cctcgcaaca cttcccttaa tgctgtacct gaacaatcta aaacgacagg
     3541 ctttggctga caaacttgca gcatttgtcg ataaaaatca gcaggcaatc cttctggcaa
     3601 gcttcctgaa aaagaaatga tttcggcttt aacagctaat ttttcgaaat gttgcaagaa
     3661 cgcccttgct tcttgttcag taataacagg tccgttttct aggatttccg tctgttttcc
     3721 gtcatgtaaa acagcgatgc aattacgtgt ctctccgctg ataggtaaaa agtcatgagc
     3781 aatctcattt tgagtcaggt cctttttaat ctcttcacct aaaaaaccgc cgattaaacc
     3841 actagcaatc acctccgtat ctaattgctt caagatacgt gtcacattta ggcccttacc
     3901 gccagctgtt ttgcgaacat ttcttactcg attgactgtg tcgagcttga aggtttctaa
     3961 tggataagaa atatcaatcg atggattcat cgttattgtt aaaatcattt gtccacccgc
     4021 ttaatcgtgg tattcgccac gatcccattt ctcgataaat tcatcgaaga aatgcggatc
     4081 agactgcttg tcactggctt tttcgacgtg atcgattttg gcgatcaatt ttttgttttc
     4141 ttcagtttct tgataatcgg cattgatgaa ggcctctaat atgtcacaga tcaaaaattc
     4201 tcccgtaatc cgagcgccaa agccgatcac attggcattt agttgttcct tcgcatagat
     4261 agcagtcgtc atatcgcgaa ccagcgcact gcggatacca ggtactttat tgacagcgtt
     4321 gttaatccca acacctgttc cacacaaaca aactcctaga tctgcttctc cgctagtcac
     4381 ggcctctccg actttttttc cgtagatcgg ataatgggtt ctggtaaagt cataggtccc
     4441 catatccaac acttcatgtc cttttgattt taagaaatct gatactgcca tttttgtatc
     4501 tgtgacgatg tggtcacagc caattgcgat tttcatttca ttcatccttc ctaattacgc
     4561 cattttattt aacatgtcga cacgtacttg atgacgacca ccatcataat ttgcttgtag
     4621 aaattcctta acaatattca acgccaactc atcgccaaca atttttgatc caagtgtgat
     4681 catccgagaa ttattatgtt tacgggtcat atacgcactg cgctcatccg aaacttctgc
     4741 tgctaccatt cctttaacct ttgttgccgt gataaagctg ccagcgccgt agccatcaac
     4801 cgcgattcct aaactacctt cgttttcatt cagatcattt gcgatcgcca aagttgattc
     4861 cacaaaatct tctgaagccg tttcactctt atctactact gtaatacctt ctttgatcaa
     4921 ttttttcttg attaattcct ttaattccat gccgtcactg tctgaaccaa ttactacacg
     4981 catttttgtt tcctcctatt ttttactgaa tgattttagt gaattgctga taataactct
     5041 tcagctcggc tgaaagagta gaatccgtaa ataatttgtc gatcttgcgc aaatcataga
     5101 aattataaaa atcgctatgg tcgaatttgt ttgcatctga aaccaaacat ttttctgccg
     5161 cattatctaa aacaattttt tgcaaggtac cttcttcaat actgaaattc gaaacctccg
     5221 aacccttcaa tccattcact ccgacaaaag ccttcttaat accgattttt tgaacagctt
     5281 cattggcaat tgaaccgaca aaggctccag tcttttcccg atatagccca ccgatcaaat
     5341 acagttcaaa tctttttatt tcacttaata gattgaaaac cggcagacta ttggtaacga
     5401 ttcggagaga tgacggcttg atgtatcgtg tcattaattc gatcgttgta cccgatccaa
     5461 gaaaaatcgt gtcgtcttct tgattgatat gagctgccgc atttttagcg atgtattcct
     5521 tctcttttgt atgcagcttc tttttttcag tatgagaaag ctcatttgct tctatagaag
     5581 tcgacatttc cagataatcg attttttgtg caccgccatg aacgcgtaaa agctttttct
     5641 tctgcgcgag ttcctctaga tctcggcgga tcgtcatatc tgaaacattc agtacttcct
     5701 gaataaattt gactgttatc gtcccatagc gatcacacat cgctaagatc gaacgctgtc
     5761 gttctgtttt cagcatattc tgtacctcca cgaccttata ataaaacatt tacgaacatt
     5821 tttcaacaca tattgttcgt ttattttcgt ttatgcgttg acaatgattt tgtaagcggt
     5881 tatactcaac ttgtaaacat ataaataaac ataaacgaac taataaagga gctgttttgt
     5941 gtgaatgaat tctttttcaa agacactgtc tatgtttctg acaagcagtc gcaggaagaa
     6001 gtgttcgagg aagtctatca agatctgtta aagaaagaat tggtaaccga agattttttg
     6061 gagaaccttt tagaacggga gcgtaattat ccgacgggat tagatctctc acctgtctct
     6121 aaaatattgc cagatatcgc tatccctcat accgaaagtg agtttgtgcg aaccacccgt
     6181 atcattccaa tcaaagtaaa aaatgaaatc cctttctaca acatgatctc gccaagcgat
     6241 cagatttccg cttcatttct tttcatgatc ttgaacgaaa atggtgaagc acaggctggc
     6301 atgttggcta atatcatgga tttcattaat tctgttgacg aagaaaaact gttttcgttt
     6361 ttcaaattag aggacacaga agcgatttat cattttttag aagaacattt ttatatatag
     6421 aagtctctgc ctaaccgcag gataaaaaat aaacttattt taaggggaga attattatga
     6481 tcaaaatttt agcagcatgt ggagcaggcg ttaattcaag ccatcaaatc aaaagcgctt
     6541 tggaaactga attgaaaaat cgtggctatc aagtcacctg tgacgccgtt atgatcaaag
     6601 atatcaatga agaaatgctg tctcattatg acattttcgc acaaattgcc aacaccgact
     6661 tgggttttga cattaacatc cctgtcgttg atgcaggtgc tattctttat cgtattcctg
     6721 ctatggctga gcctgtttac caaagtatgg aaagcgcaat ccaatcatta aacaaatagg
     6781 actaaagaaa ggaaaagcat atgaatacaa ttattgatat tgccaataaa gtatttaccc
     6841 ccctaattaa tttaggtgcg gcaccgttaa tgaccatcgt attaactgtt attgctttat
     6901 tttttaaagt aaaaccgtca cgcgctcttg aaggcgggct caaattaggg atcgccctga
     6961 ctgggatcgg agctattatt ggaattttaa ctactgcttt ttcagacgct atgacagctt
     7021 ttgttgaacg cacaggtatt tcattaaaca ttacagatgt cggttgggcc ccactagcaa
     7081 ccatcacatg ggggtcgcca tacacgcttt attttttact aattatggtg atcgtcaata
     7141 ttgtcatgtt agcaatgaaa aaaaccaaca cattagatgt ggatattttc gatatttggc
     7201 atttagcaat tgttggtttg ttcgcgatct tctgcggtgc taatctgttt attgcttctg
     7261 cattagttat tttcattggt gtattaaaaa tttggaactc cgacttaatg aaaccaacct
     7321 tcaatgactt gttaaatgcg ccagacagca acccgatgac cacgactcat atgaactaca
     7381 tgatgaatcc gattattatg gttttcgata aaattttcga caaaatcttc ccttggttgg
     7441 ataaatatga tttcgatgct gccaaattga atagtaaaat tggcttctgg ggatcaaaat
     7501 tcgcgattgg aatttatttg gggatttttg ttggattact agcaggacaa acaccaacgc
     7561 aaattttctc gctagccttt accgctgccg tctgtttgga attgttctct ttgataggcg
     7621 cttggttcat cgcagccgta gaaccgttat cacaagggat taccgatttt gccaataaac
     7681 gactaaaagg acgtaccatc aacataggtt tagactggcc tttcatagca ggccgcgctg
     7741 aaatctgggc tgccgctaac gtattagcac caatcatgtt gctagaagcc atcatcttac
     7801 caggaaataa attattgcca ctaggcggaa tcatcgcaat gggtgttacc ccagcactat
     7861 tggtcgtaac tcgtggaaaa ttaatccgta tgatcgtaat cggcgcgatc gaattaccat
     7921 tattcttatg gtccggcacc ttgatcgccc cgttcgttac tcaaacagct aaagcagtcg
     7981 gagcctttcc agccggcttg aacgggtccg ccatgatctc tcatacaacg atggaaggcc
     8041 cgattgaaaa attcctaggt tatttagtcg gtaatgcttc tcaaggacaa atcgaatttg
     8101 tgatatacgc tgccttagca ttaggtgcat accttttaat cttcatttgg tacgctcgtc
     8161 aaatgaaaaa acgcaacgct gcatacgcag ctgaaaaaga agagaatgca tctccggcaa
     8221 cggcgcataa tatgacttat gcagccgaag ctaaataagc taaaaacacc ccgaatgtct
     8281 ttagacttcg gggtattatt ttacctataa aaaataaata gatgtaaaat ccatttcaaa
     8341 aagcatagcc catctcttgc cacacccaat aaagaaatta tcaacgttta tgcttttttt
     8401 aactccttta agcttgcctt tgttggtatt cctcaacgat tctttttgtg ttcttgtttg
     8461 ttttgtgctt tttgactttt tgttgtttaa ttgcgaattc cttgatttct tttttcctga
     8521 atcgcttcgc atcccgcggt tatgcgtgcg atttcatcat tgctttttct gatctaactc
     8581 gtttcatgtc aaagagcttc agctgactta gtgtccatgg ttgttttgat atcccttttc
     8641 ctccttaata aagcggtgat tgatactgct attttttcct tcacataaac ataacatccc
     8701 aaaaaaatcg actatctttt ttcaaatggt gtattcaagt attcggagga attagtaatg
     8761 atgcacccta aaaataacat aattcgctta aattcaaggc taccaaaaaa ctaccaagtt
     8821 ctaattatct agagtttggc agtttctcat ttacacaaca tatttttctt ctttttcgat
     8881 tttttcatag tgcttttgtt catcatcatc cgttataact actgttacat cattcagatt
     8941 ataatatact gaaaaatctc ttttcccaat tttcgaatga tcaatcaata aaaagcgttc
     9001 aacagaatta ttcagagcga tctgttgagt ttggccctct tcaaaagtcg aagtcataat
     9061 tttctgttca tttaaagcat tacagctgaa aaatgcttta tgaaaattca tatcctgtag
     9121 cgatttattc gtgatttcac caacaaagca ttgagtcgtt tcccgcatct cgccacctaa
     9181 tagatagatt tttcgattcc cttttcgctt atttaattct tcaaaaacag gcaaacagtt
     9241 tgtaacaatc cgcagtgaat ccccttgaat gatttttgac atgagctcga ttgtagttcc
     9301 tggtccaaga aatatcgtat caccatcatt gatcatttca ttcgcctttt ccacaatccg
     9361 ctgcttttct tcttgcagca taatctgttt ttctctatga gaaagctcca catgccgata
     9421 aaaatccttc aatttcgctc cgccatggac gcgttctaat aatccttgtt tctccatttc
     9481 gtccaaatca cgtcggactg tcatatccga aaccgaaaac ttctccataa tatcgctgac
     9541 tcgtactgta ccgttaacct ctaataacga catgatcgct tggaatcgat ccgtttttaa
     9601 catttcatcg cctcttttct ttttaagact atcaaattct taagaaaagt gctagaacaa
     9661 gttttcttaa gaagccttct gcaggaagga aagcaaaatt tttcagaatt tgcaagctgc
     9721 tattctaaaa aataaaaccg aacgagcatg actgtcatag tgacagcctt gttcattcga
     9781 cttttatctt taataaatca ttttatttaa tctgaagtta ttgtgcaagt tcaaaaacaa
     9841 agtagttaat gtctcctcga tatttcgaaa tcgaatactc aacgggcaga cgttctagcg
     9901 aaaaaccctg tgtatggaaa tagaacattg gatctccttc ttccacatct aacaaatcgc
     9961 tgatcgtatc atccgctttg attacttcta gttttctggt gaccgtttta attggataac
    10021 ccatttcttc aaaaacttga tacatttttt cttttctgat ttgtcaaatt aagctagaca
    10081 atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa tttaatgatt
    10141 ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca tcc
//
LOCUS       DACDID010000095        10113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDID010000095 DACDID010000000
VERSION     DACDID010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.095"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            41..547
                     /locus_tag="IV236_001917"
     CDS             41..547
                     /locus_tag="IV236_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8772100.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            564..1463
                     /locus_tag="IV236_001918"
     CDS             564..1463
                     /locus_tag="IV236_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8772101.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1550..2434
                     /locus_tag="IV236_001919"
     CDS             1550..2434
                     /locus_tag="IV236_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8772102.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2626..2697)
                     /locus_tag="IV236_001920"
     tRNA            complement(2626..2697)
                     /locus_tag="IV236_001920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2662..2664),aa:Arg,seq:ccg)
     gene            2757..4091
                     /gene="rpoN"
                     /locus_tag="IV236_001921"
     CDS             2757..4091
                     /gene="rpoN"
                     /locus_tag="IV236_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8772103.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4294..5331
                     /locus_tag="IV236_001922"
     CDS             4294..5331
                     /locus_tag="IV236_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8772104.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5372..6373
                     /gene="gap"
                     /locus_tag="IV236_001923"
     CDS             5372..6373
                     /gene="gap"
                     /locus_tag="IV236_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8772105.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6483..7673
                     /locus_tag="IV236_001924"
     CDS             6483..7673
                     /locus_tag="IV236_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8772106.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7777..8532
                     /locus_tag="IV236_001925"
     CDS             7777..8532
                     /locus_tag="IV236_001925"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8772107.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8648..9946
                     /gene="eno"
                     /locus_tag="IV236_001926"
     CDS             8648..9946
                     /gene="eno"
                     /locus_tag="IV236_001926"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8772108.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3531 a         1874 c         2105 g         2603 t
ORIGIN      
        1 ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta ctggatataa
       61 gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg aaactgatta
      121 tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg ccttagatgt
      181 gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag agatcgagca
      241 ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt ggcgcagaca
      301 aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt tacctttaga
      361 aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg aaaaagagat
      421 tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta tcttggatat
      481 gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac gtcaaatcct
      541 agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga
      601 aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc cagcgtttta
      661 ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc caagggttcg
      721 gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat agtcaagttc
      781 gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc ttaggcgaac
      841 gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat acacaaaatg
      901 agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa ggacaagcag
      961 attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac caagttttgg
     1021 gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg cagatcaccc
     1081 aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg
     1141 tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt ttgacaaaag
     1201 aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac tattggactg
     1261 agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct acacgaagtt
     1321 tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa attgctcctg
     1381 gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg attttggcat
     1441 cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt caattttcaa
     1501 gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag tgaaaagaaa
     1561 ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt
     1621 ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt cgtcacaatc
     1681 tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag ctaacgtcaa
     1741 agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat caacacaaga
     1801 cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct ttgatgatgg
     1861 accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc
     1921 cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga agcagacagc
     1981 tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg ccactctttc
     2041 tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa aagtcactgg
     2101 caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag gagcaacgat
     2161 catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga caagaaatcc
     2221 tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta ttttattcca
     2281 tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca
     2341 agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg tcgagaacta
     2401 ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt taataaataa
     2461 cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt aacaatatct
     2521 atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt tcacttaaaa
     2581 ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt cctgagggaa
     2641 tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta aggacgcaag
     2701 cactgggata attgtaacta aacagataaa aaattacaag gggattggac aaaaaaatga
     2761 aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc atgacgcaac
     2821 agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg gcattcgttg
     2881 agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg cagccagatt
     2941 acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta aatcagattc
     3001 ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg aattaccgag
     3061 atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg aacggatttt
     3121 tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc caaatgctcg
     3181 atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt agtttgcaag
     3241 aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct tatatcgtgt
     3301 tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca caacgttttt
     3361 ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg agtccaagcc
     3421 ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc cgggtattag
     3481 tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca aatatacgct
     3541 ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact gaaaattatt
     3601 taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa cgtggggata
     3661 caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt atcgacaagg
     3721 aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat gtgcatgaat
     3781 ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt gtatttgaat
     3841 taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca gaagaccttt
     3901 cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac aaaaacaagc
     3961 cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg atctcaagac
     4021 gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa agaaaacgtt
     4081 atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag tctttttagt
     4141 tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt ccctttattg
     4201 atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt taagtctaca
     4261 ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa aatgattgaa
     4321 tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt acgaaatatc
     4381 tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat cacagaacga
     4441 gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac ctcaaaaagc
     4501 ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat
     4561 cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg taaccaacgc
     4621 tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc attcggcgaa
     4681 gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat cgctgtgatg
     4741 ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa taagaaacgg
     4801 cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt acaggcaaat
     4861 tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact ttatgttccg
     4921 gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat ccaagaagtc
     4981 ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc attacacatg
     5041 gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt
     5101 gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga
     5161 atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga
     5221 aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg caccaaaaca aacgtggctc
     5281 atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta aaataaatat
     5341 ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta ttaatggttt
     5401 tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa tcgaagtagt
     5461 agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat acgacacaac
     5521 tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg taaacggaaa
     5581 agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg aactaggcgt
     5641 agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg aaaaacattt
     5701 aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg ttccaacaat
     5761 cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct caggtgcttc
     5821 atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat ttggtgttgt
     5881 tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc tagacggacc
     5941 tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc
     6001 aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta aattagacgg
     6061 agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa cagttcttga
     6121 aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta
     6181 cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt acggttcatt
     6241 atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag ttaaaactgt
     6301 tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt tagaatactt
     6361 cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta catttaaaag
     6421 cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca ggaggaaata
     6481 aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg
     6541 ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt atcaaagcag
     6601 cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt ttctctcacc
     6661 ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca gttgcaaaac
     6721 gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat
     6781 tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa aatacacgat
     6841 ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt aaatactggg
     6901 cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt gcacatgctt
     6961 ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg atggaaaaag
     7021 aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt gcaatccttg
     7081 gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct aaagcagata
     7141 aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga atcaagatcg
     7201 gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc gaaaaagcag
     7261 gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca aatgatgttg
     7321 aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac atcggcccag
     7381 catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt tggaacggac
     7441 ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa
     7501 tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagcc gcagcagcag
     7561 aacaattagg atttgctgac aaattcacac acatctcaac aggtggcggc gcaagcttgg
     7621 aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa taaacgacca
     7681 agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc atgactccct
     7741 agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt
     7801 aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac
     7861 aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt attcttacaa
     7921 gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg
     7981 gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga tctaggtgtt
     8041 cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac agacgaagac
     8101 atcaataaaa aagcgaaagc tatctttgca aatggtatga caccaatcct atgctgtggc
     8161 gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg acaaattaca
     8221 gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc ttatgaacca
     8281 atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga aatttgtgga
     8341 gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc agttcgtatc
     8401 caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa agaaaatgtt
     8461 gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc attgttagac
     8521 gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca
     8581 aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact caaaggagag
     8641 acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact cacgtggtaa
     8701 cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg gaatggttcc
     8761 ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag acaaagctcg
     8821 ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca ttgctgaagc
     8881 aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga tcgctttaga
     8941 cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt
     9001 tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag gcggattcaa
     9061 cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac atgctgataa
     9121 ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat tcaaagaagc
     9181 tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag ctcgcggatt
     9241 agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa acgaagaagg
     9301 ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta aagacgttgt
     9361 tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact
     9421 agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct acgaagaatt
     9481 agtttctaaa tatccaatca tctcaatcga agacggatta gacgaaaacg actgggacgg
     9541 attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg acgacttgtt
     9601 cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta actcaatcct
     9661 tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg aaatggctaa
     9721 agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag attcaacaat
     9781 ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc tatcacgtac
     9841 tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg gtgaagttgc
     9901 agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct tttctaatga
     9961 agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa gtaatcgaac
    10021 tctctacttg ctgaaatatc ggctctttgt caataaggac tgatgagttg tgcaaaatca
    10081 aatctgagtc agaatgaacc acattctggc tca
//
LOCUS       DACDID010000096         9922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDID010000096 DACDID010000000
VERSION     DACDID010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.097"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..3332
                     /gene="fss3"
                     /locus_tag="IV236_001927"
     CDS             105..3332
                     /gene="fss3"
                     /locus_tag="IV236_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8772109.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3445..3759
                     /locus_tag="IV236_001928"
     CDS             3445..3759
                     /locus_tag="IV236_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772110.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3772..4146
                     /locus_tag="IV236_001929"
     CDS             3772..4146
                     /locus_tag="IV236_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772111.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4156..4491
                     /locus_tag="IV236_001930"
     CDS             4156..4491
                     /locus_tag="IV236_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8772112.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4574..5914
                     /locus_tag="IV236_001931"
     CDS             4574..5914
                     /locus_tag="IV236_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8772113.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5992..6666
                     /locus_tag="IV236_001932"
     CDS             5992..6666
                     /locus_tag="IV236_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772114.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6899..7333
                     /gene="dcm"
                     /locus_tag="IV236_001933"
     CDS             6899..7333
                     /gene="dcm"
                     /locus_tag="IV236_001933"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8772115.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7334..8041
                     /locus_tag="IV236_001934"
     CDS             7334..8041
                     /locus_tag="IV236_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8772116.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8031..8321
                     /locus_tag="IV236_001935"
     CDS             8031..8321
                     /locus_tag="IV236_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772117.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8580..9764
                     /locus_tag="IV236_001936"
     CDS             8580..9764
                     /locus_tag="IV236_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8772118.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9779..9898
                     /locus_tag="IV236_001937"
     CDS             9779..9898
                     /locus_tag="IV236_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8772119.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3286 a         1674 c         2245 g         2717 t
ORIGIN      
        1 atccattcta ttcagtcaat aaaaatttta aaaagacttt ataaacaaag atgttcaagc
       61 gagcatctta tttttttgtg cttaatttga aaggagaaca tttcatgtat aaaaaacaaa
      121 aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt gttggagcaa
      181 ttgctcctac gattactttt gctgatgaaa ttacccatcc acaaacggta accgtagaac
      241 tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag
      301 aagtcactgt tccgcattat gcagtgtaca acggctttaa acaagatatt ttctgtattg
      361 aaccaggagt cccgatttac aatgagttca ctcctggcta tgagaaaaac ccgttaccag
      421 atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag
      481 atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact
      541 caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaataaatc
      601 aagccattga cgattatcag aagaaaccaa ccttccataa tagtacggct aaaacagtat
      661 tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg
      721 ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg taatcagttg ataatcaccc
      781 caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct ggtaccggaa
      841 cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg attgataaac
      901 ctaataccta cacaatcaaa attgatgtgg aaacggaggg ttctttaaag atcaaaaaag
      961 tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac tttggaaaaa
     1021 ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg gatgagattc
     1081 ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat acgattgata
     1141 ccacccccat gactaccacc attaaagcag gcgaaaccat ttacgtgact tcaaaaaacg
     1201 cacgggaaaa agggcaaatc attctggata aaagcggagt tgaaacaggg agtgatcttt
     1261 ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac agtccaaccg
     1321 gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca ccaaaagaga
     1381 ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct agtcatgggt
     1441 tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta tgccaatcaa accgtagccc
     1501 ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc actttgacga
     1561 aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag ggtgctgaat
     1621 acaccttgtt tactgctaag gatggaaaag ctgttaaatg gagtgaggct tttaaaccag
     1681 agttagcgaa gggaacgaaa ggttctgatg aaacggtcac tttgacttta gacgaaaaga
     1741 accaagttgc cgttaaacat ttagctatca acgagtatta ttggcaagaa acaaaagcgc
     1801 ccgaaggata tagccttgat gaaacaaagt atcctgtatc catcaaaaag gtggatgata
     1861 gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa agaacaagtg attcgctttg
     1921 gctttgattt ctttaaattt gctggatcag ctgcgggcac taccgaaact ggcttcaatg
     1981 atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt gccgaagatg
     2041 aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc
     2101 ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta
     2161 cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg aagagtgagt
     2221 atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg accgttcctt
     2281 acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg ctctatgatt
     2341 tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga aacaaagaat
     2401 taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta catgaaatca
     2461 aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa gctgcccaag
     2521 aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg ttggcgaaca
     2581 aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa gttttaaata
     2641 aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa gcaggcgata
     2701 agccagtagc gaaggatgtt agcttgaata accaagccca aactgtcagt tgtacagtgg
     2761 aacaccatgt ttctatccaa acaaaggccc acctagaaaa cggttctcag acctttactc
     2821 atggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta ctggatggct
     2881 caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca aacaaagaaa
     2941 tttggaaatc tggcaagatt gattatgaag taaatgataa agaattcacc aaaactgtgc
     3001 ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact tttgcggaaa
     3061 tcaattacga taaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat
     3121 ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa caaccggaaa
     3181 caccaactgt tccaagtggc tctcaagaat ctagtcccac agtgaagacc ttccctcaaa
     3241 ccggggagaa aaattccaat gttttactgt tcattggttt tactttgatt tttgcgacgg
     3301 cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa gtaaatccta
     3361 agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg tatccaaaac
     3421 taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga tatggctgaa
     3481 acctttggaa agttaagtta tgccggtgaa ggagaaatta taacagaagg atatggacgg
     3541 aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg ggccgatgat
     3601 attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga ccaaccgcta
     3661 aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg tgggtttact
     3721 gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa gatgagatta
     3781 acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt ttccgcatta
     3841 cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt aaaagaacga
     3901 acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat cccagctaat
     3961 gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc aatcgtggat
     4021 accgttgcca attatgtttt tcgagaagga actaccgtta attggttcat taaggcggat
     4081 gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa tgaaagaaag
     4141 aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct cgatcaatat
     4201 ggaaaaggcg agctgactga ggaacaactc gttaaagcat taacctctga tgaaaaaatg
     4261 tttctgatta tacatcaagg tttacttgga gaaaatagcg acagtgaaga agactctgat
     4321 gtactagact ggttaggaga agaagagtct ttctttatag ttgtcgaagt aaatgaaaac
     4381 ttatgtcggc aggcagaatc cattttggaa gaaattggtg tggagatgcc ggatgctatt
     4441 gaaggctttt taaatcaatt ggttgaaacg aaacaactcc cctcctcgta gcagtaaatg
     4501 actagcataa ctttttgaaa ttgcctaatg aaatcctatt gggctttttc gattggaggt
     4561 ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcagc acttggttta
     4621 tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt
     4681 tcaagagttt agacaatttg atatttctaa gctatcactt tctactattg aaatcgtttg
     4741 gtctataaaa gatttagtat gtctgcttgg aagtctcgct ttttcaggag ctatgatatt
     4801 gctttatatt cacttttttc ttgatcattg gcgaagcttg tggcatcgac aaaagctagc
     4861 tcggatgatt ctagagaacc attggtatga agtgaaacaa actcaaagtg aaggcttttt
     4921 caaagatttg aatagtagcc gaaccagaga gactatcagt tacttcccca aaatctgtta
     4981 tcgaatgaaa gatggattac tgtctattcg cgttcaaatt tcacttggga aataccaaga
     5041 tcagctttta aaattggaaa agaagttaga aagcggactg tattgtgagt tagtagaaaa
     5101 ggaactcaaa gactcctatg tggagtatac tttactgtat gatatgatcg ccaatcgaat
     5161 tgggatagac gaagtagtgg cagaaaatgg gactctgcga ttgatgaaaa atcaagtatg
     5221 ggcttatgat tctttacccc atatgttaat cgctggtggc acgggtggag ggaagaccta
     5281 tttcctcctc accattatcg aagcactatt gaagtcagat gcagaattgt ttatcctcga
     5341 tcccaaaaat gcagatttag ctgatttagg tacggtgatg cctcatgttt attcacaaaa
     5401 ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt atgatggctc gtagcaaggc
     5461 aatgaaagaa atgtctaact acaaaacagg agagaattat gcctatcttg gacttccacc
     5521 caacttttta atctttgatg aatacgtggc ttatatggag atgttaacga cgaaagaaag
     5581 tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg ggtcgtcaat ctggtttctt
     5641 tctgattttg gcttgccaaa ggccggatgc gaaatattta ggagacggaa tccgcgatca
     5701 attcaacttt cgcgtggctt tgggtcgtat gagtgaactc ggatattcca tgatgtttgg
     5761 ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt cgaggttatg tggatacagg
     5821 tggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg atttcttaga
     5881 ttcaattagt aaattagaaa agatgaatga ttgagtgatc ttcaatactg actttatact
     5941 tcaagtaacg tataatactt ttatatagta ggaagaaagg ggataattaa tatggaaagt
     6001 aatgattctt atttagagta tgcaaaagca aataaggata agtttatttc ttatattatc
     6061 aaagggaaat cccccgaagt tggacgagac gcagttttta tggcagggag tcctggggct
     6121 ggaaaatcag aggttgccat tggattagct gagaattatg ataaccatgt gattattgat
     6181 gcggattact ttcgcaccca gtttcctgat tataatggaa aaaactctag tctctttcaa
     6241 aaagcatctt cttggttggt agagcaggca tttagatatg tcttagatca tggctattct
     6301 tttattctgg atgcaacttt tgcaatatta agtgctgaaa aaaatatctc gcgggcgttg
     6361 aaaaaacatt ttcgagtgac gattttctat gtttatcaag accctaaaat cgcttggaaa
     6421 tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc cgaaaaaggc atttattaac
     6481 gcttttttca aatcaagaga gaacattgca aaagtaaaga gtaaacatcc agatgtattg
     6541 cttcatatca taataaaaga ttatacgaat aatatttccg aagttcatta tgctacggat
     6601 aatctagaat tatttttacc actagggtat agtaggaaag atttggaggt tcaattacat
     6661 gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac aataagagca ttgcggattt
     6721 atcggaaaat gctactgcaa aagagttcaa aacggtgatt aaatatgtag ccgatcaggc
     6781 caatgaaaaa caacggaaat tagttggatt aaataaaaaa tagtaggaca aattaataat
     6841 gtatgagcca agagcaaaat gttcttggct tttttctttg caatgaatga ggtaatgtat
     6901 gagattttta gatttattcg ccggtattgg tggctttcgt ttaggtatgg aacaggcgag
     6961 ccatcattgt atcggttttt gtgaaattga taaatttgct agacgtagtt ataaagcaat
     7021 tcatgataca agtaaggagg tggaaatgca tgacatcaca agtgtatcag atgagtttat
     7081 tcaatccctc ggaccagtgg acatcctttg cggcggattt ccgtgccaag ctttttcaat
     7141 tgcgggaaag cggcaaagat tttccgatac tcgaggtact ttattctttg aaatcgctcg
     7201 gttcaccact cttctccgac ctaagctttt attccttgag aacgtccgag gattgctcaa
     7261 tcacgaagga ggggctacgt tcgagacgat cctccgaacg ctggatggat tggggtatga
     7321 cgtggagtgg taggtgctta actcaaaggc ctacattccc caaagccggg agcggatatt
     7381 ccttatcgga cattctcgag acgcatgtac cgaacaagta tttcctatca ttggaccgtc
     7441 ttcaacatct gatcaaaaca tcagaaattt ggtaaatatc aatccttcca atcgaggaat
     7501 gggtggacaa gtctatggtt cagatggcgt agcgccaacc ttaacaggtg atgaaggaat
     7561 caaaatcgca ctgccagcaa gggatggaat ctctgtagca ggactattac cgggaaagtt
     7621 tgaacaaggt aatcgagttt atgaagttac tggaaccgcc cccaccttgt caacgaaaca
     7681 aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa tatcaagagg tgaagccaga
     7741 agacagtgtg aatctggctt ttcctaattc cacaagtcgc cgaggtcgtc tgggaaaaca
     7801 gagtgttcat accttactaa caggagacca acaagcgatc gtcacggatc agtatcaaat
     7861 tcggaagcta acacctagag agtgttggcg acttcagggc tttcccgatt gggcttttga
     7921 tcgagattct caagtcaatt cagatagtca gttgtataag caagccggaa actctgtaac
     7981 agttccggtc atttttgata ttgccagaag attaaaagag ttggatgtac atggtttatg
     8041 atgagttgat tggagaaatt tattgggtga ttggaaagat tcagtcagac ccagaattag
     8101 aagaacaatt gcgacggtta aaatttgata ttcgcaagaa tggtgtcaaa gtcgctggcg
     8161 atccatattt aatgaatgag gagacggatg ctcgaattga aataaatcaa gtaattgcag
     8221 aatttgagcg gattgcggac caagcaaaag aaccagatat tcgtcaatac ttgttagaaa
     8281 tgaaagcaga gttggaagta aatggtatta cagatgagta acctaaacca acacaactga
     8341 ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat cctccgtggt gctgtgcctg
     8401 ttcatggacg gcgttagccg accatgatgt ctcacccccg tatctaacag gggggtacaa
     8461 aaacacaaaa aataagtaag caaccattaa gaatgcttgt tgtatcaaag atagtaagta
     8521 atttgtatag gtgttaactc atttgaaaaa gttaacacct tgaatttgga ggtgagaact
     8581 tggcacaacg gaatttagat tatcggctat taaaagaccg acgcaacgaa tacggcgttt
     8641 cacaaaataa attggctacg gcttgtggac ttagtcggcc gtacttaaat cagatagaaa
     8701 atggtggcgt aaccgcatct ataaaaacca tgaggaaaat ttttgaccaa ctggaaagct
     8761 ttaatcccga tttgcctttg acgttactgt ttgactatgt aaggattcgc tttcccacaa
     8821 cggatgcacg aaaaattatt caagaaattc tccatttgaa atttgagtat atgctccatg
     8881 aagattatgc cttttactcc tatcaagaac agtatgtcat gggtgatatt gtggtgatgt
     8941 tgtctcatga agaagataaa ggtgtgcttt tagaattaaa ggggcgaggt tgccgacaat
     9001 ttgaaacttt tttactcgct caaaagcgta gctggtacga ctttttcgag gattgtctaa
     9061 aggccggtgg cgtgatgaaa cgcttggact tggcaatcaa tgatcgagtg gggctattgg
     9121 atatacctga gctaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9181 ttaaaagtta tcgttccggg gaacttttaa aggctgatga aaaggacggc atgggcaata
     9241 ccttatatat cggcagtctc aagagtgagg tttatttctg tttgtatgaa aaagactatg
     9301 aacaatatat caagttaggg attccattag accaaactga gacgaaaaat cgttttgaga
     9361 ttcgactgaa aaatgaccgt gcttttcatg cgattcaaga tttgttaaaa ggtcgtagca
     9421 ttgagagcac cacgttttcc attatcaatc gttacttgcg atttgcggat aaagtagaag
     9481 gtaaaaggcg aaccaactgg cccttgaatg aacaatgggg gcgttttatt ggacggaatc
     9541 ggaaagagat tcaattaact tccgagccaa aaccgtatac cattgaacga accttgaatt
     9601 ggctaggccg acaagtcgcc cctacgtgga aaatggctaa ggagttggat cgattgaatc
     9661 ggacaaccta tatccaggat atggtacaga atgcacgctt atcagaccga cataaaaaga
     9721 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtcat
     9781 gggaattgta aaagatattt ttttggtatt tggtggcagt atgcttggtg ttactgtgat
     9841 gtgcttgatg aatgcaagtg cagcagcgga tcgagcaatg gaaaagaaag gaaagtgaca
     9901 agatgaattt cggacaaaat tt
//
LOCUS       DACDID010000097         9922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDID010000097 DACDID010000000
VERSION     DACDID010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.098"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(133..339)
                     /locus_tag="IV236_001938"
     CDS             complement(133..339)
                     /locus_tag="IV236_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772120.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(355..969)
                     /locus_tag="IV236_001939"
     CDS             complement(355..969)
                     /locus_tag="IV236_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8772121.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1116..1799)
                     /locus_tag="IV236_001940"
     CDS             complement(1116..1799)
                     /locus_tag="IV236_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8772122.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1908..2366)
                     /locus_tag="IV236_001941"
     CDS             complement(1908..2366)
                     /locus_tag="IV236_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8772123.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2450..4213)
                     /locus_tag="IV236_001942"
     CDS             complement(2450..4213)
                     /locus_tag="IV236_001942"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8772124.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4434..5894)
                     /locus_tag="IV236_001943"
     CDS             complement(4434..5894)
                     /locus_tag="IV236_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772125.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6102..7028)
                     /gene="glsA"
                     /locus_tag="IV236_001944"
     CDS             complement(6102..7028)
                     /gene="glsA"
                     /locus_tag="IV236_001944"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8772126.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7051..8478)
                     /locus_tag="IV236_001945"
     CDS             complement(7051..8478)
                     /locus_tag="IV236_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8772127.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8509..9723)
                     /locus_tag="IV236_001946"
     CDS             complement(8509..9723)
                     /locus_tag="IV236_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8772128.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2944 a         2053 c         1641 g         3284 t
ORIGIN      
        1 ttttttagaa aaaaataaca aataagttga aagaatcccg ttgcaaattc tttcagttgc
       61 accaggattc tttgataatt ttttagtttt atgtgttttt ttatatgttt tttcactttt
      121 cggaataaat atttatttgt taaagtaatt aaacaatccg ctaatcgttt gatctggcat
      181 ttctatttcc ttaccatcta aagtaacagt agtaacgaca aaatggtctt gcaatggcgg
      241 aacttctaag tactgccctt gcagaaaatc attcactgtt tcatattctt ttttcatttc
      301 ttttgaatct gtcgatggtt tatagcttat ttttaacata actagtgctc ctttttattc
      361 gggacgtgca atcgtgaaaa tttctcctat tgctgcataa tcatttcctg ccaaacggct
      421 tacagcattc aattttcttg gatcgatcct tcctgtctta tcatcataga ctgtttcatt
      481 taaatgatat ttctgtactt ctagcaaaaa taaatcagct gttactttat ttttattccg
      541 tatctcgaca tgttcatata aggatgtttc aaaacgaact tttgcttctg tcaatgctgg
      601 tactgaaatg attactgaat cagttaaagt caagtcagtc aggtacagtt cgctttcctc
      661 attaggcaga ttggctgctg ttttatttac ttcttctacg ttgtcctcat caaggatatg
      721 gataacagct tctcttgtct caagaatatt ctttgctgtg tcttttactg catcttgttt
      781 tctctggatc gccaaggata agatgggtcg attcgaactg acgatattga agtaactgaa
      841 gggtgcgata ttcactatat cttttgctga tttagtcgtt actactgcta ctggtctagg
      901 gatgactgaa ccaatcagca acttgtaatt ttcacgttct gtcaattctg aaggactccg
      961 ttcaaacatg gtttcgcctt ctttctataa aattcgttaa aaagcagatc ttgaagacaa
     1021 aatgaaaagt aatgcttttt tcatcttctt agaatctgct ttaaactatc tatcaatttt
     1081 tcttttttgt caagaatctg tgccgtgcgt gttttttagt tatttgcttt acttgcggct
     1141 tcgacatatc ttttagtgat caattgcgga cgggtgataa tagatccaac tacgactgaa
     1201 aaagcaccta attcatatgc tttctctact tgttcaggag agtcagtatg tccttccata
     1261 attactggga tatcgacttg ttctatgatt tgattcaata aatcaaaatc aggtgtctgc
     1321 gtctttcgtg aatattctgt atagcctgct aaagtcgtag acaaaagatc aaaaccatct
     1381 tcttgcgctt gaagcgcttc ctcaaaagtc gaaatatctg ccatagctaa ccgaccgtgt
     1441 tcgtgaatca tttggacaag ttcagcagtt gttttttctg tcggacgttt acgagaagtt
     1501 gcatccagtg caatgatttc tgcttgcgtc tccaaaagtt cttccacttc tttttctgtc
     1561 gcagtaataa acacttctga cccatcgtaa tctcttttga taatccctat taccggtaaa
     1621 tcaacgattc gcttgatttc attgatatct tctgtcgtat ttgctcgaat accaactgct
     1681 ccgccttctt ttgcagccaa agccattctc cccataataa aagaactgtg tagcggctcg
     1741 ttttccaaag cttgacaaga aacgacgact ccgcctttga tttgttccag tgtatccaca
     1801 atatctcctc cagtaatgtt atataaaaat acagcttcat ataaaaatgt aacccttttc
     1861 tattcaggag acaataatga gctatttctt tcagcagacc tttcttacta aaagaaaatt
     1921 acttcaaatt cttcgtatac gaccagcttc tcttctgtaa attctatgtt caacgcttct
     1981 ttataagcat gtttaaaaaa ctcagcatgg actttccgcc aataagctaa tgtccgatcc
     2041 ccttctcctt ctttccaagc atgctctttc gtgacttcac taaaaggcaa gatggtcaca
     2101 tttatatttt tgatgataca aactggttca ttttttccat tcaggaccac gtctaattga
     2161 ccaacttttg gtaaaggctc attctcttgt ttatacagat cgtaaccact tgatgtacca
     2221 gtctttatct tttttacaac tagttcgctc aactcgtctg ccattttttc tgtatcccca
     2281 aatgaaaaga aacctaattt ttctgattga taactaggat atttttcttg gaactttttg
     2341 acatattctt ctacttgctg atacataaca tccacccctt tgctttttat aatgaaaaaa
     2401 ccatccagat actagtataa acaattatct ggatggtgag cataatgaat tattgaatat
     2461 tcaagacacg cagactatct tcaacgattt gttttggtgt taaatgataa cgttcataaa
     2521 gctgccctac aggaacgcta tccgtgaatt ctttctctgc accaaaattt aatacgttca
     2581 ttgaagaatc accataataa cgactgatat tttcgccaaa tcctcctgac aaactaccat
     2641 cttctaatgt gatgactaat tcgtgtgttt cttttaattt atctaatgtt tctacgtcat
     2701 tttctgtgat cgttcctgga ttgatcaacg ttgcttcgat tccttttttt ctaaaagctt
     2761 ttactgtttc ttttcctaat tcaagaaaac cgcctaaagc taaaattgct acttttcctc
     2821 cattatttac tgtttgatag gctggaatac tataatcatt tgcgatagct gtaccatgtt
     2881 ctacaccatg tgtaggaatt cggattgcta caggctgcgc tgtttgttcg aacgcccaat
     2941 ctagcataga gatcatatcc tcttttgttg ctggagcaag atatttgatg ttcgggatac
     3001 ttgataagta aggaatgtca aatgttcctt ggtgtgttcg gtcactagct gagatcgttc
     3061 cgccaccgac taaaataatc actggattct cgttgatcgc taaatcatgt gataattgat
     3121 catatgcccg ttgcaagaaa gtacttgatt ggaagacgac tggacgtact ccctgagaag
     3181 ccaaagcaga agcgaaagtg atagagtgct gttcagcgat ccccacgtca aaataatgat
     3241 ctggatgttt ttcttggaat gttttcagtc caaaggcgcc tggtatcgct gcattgactg
     3301 cggcaatagg agctgtactt gtttgcattt tcttatccaa ataatccatg atgatcatat
     3361 taaatgtttc tccagaagtt acttgctttg cctccccggt ggacagatca aatggtacat
     3421 gccaatgata tttcatttta ttatcgattg ccggctgata accgcgacct ttttccgtat
     3481 ggacgtgaag tacaatcgga tgatcaatat cttttacttc ttcaaataaa cggatcaccg
     3541 tttccagatc gtttccatta tccacgtaac gataatcaaa tcccattgct ttaaataggt
     3601 tattgctgct tttaccgttg gtttgacgaa gctcagctaa gtttttatat aaactgccat
     3661 gattctcatc aatagacatc tcattatcgt ttactaatac gattagattg gattgtaaaa
     3721 caccaccatt gttcaatcct tcaaaagcta agccaccact tagggagccg tcaccgataa
     3781 atgctaccac atttcctgat tctttcttca gatctctcgc tttagctaaa cctgttgcta
     3841 agctgataga tgtagatgtg tgccccactg taaaatagtc atgtttactt tcatgttggt
     3901 tggaatatcc gctcacttca cggtaatgtc ctttttcaaa accatttttt cgaccagtta
     3961 aaagcttatg accataagac tgatgggaaa catcccaaac gattttatcc tttggtgaat
     4021 caaatacata atgaaacgca atcgttaatt ctactacacc tagattcgga ccaacgtgcc
     4081 cacctacatc gctgtccttt tgcaagattt tttggcgcat ctcttctgcc aactgtttca
     4141 tttcctcaac agataatttt ttgacatctg cggggctatt gattgcatcc aatacatgta
     4201 atactgtact cataatacat tctctcctcc gtactatctt cttttaatga aaatagattt
     4261 gttacacttt atctattcta ctacttagag ttgactctaa gtcaatagca ttttaaaaaa
     4321 atttcagaga aagcttgtta tataagtaga tcttcgcacg aatcaggact caaataaaaa
     4381 ccttctacag acaattaaaa agctttgtag aaggtctcct aattaaggtc tcatcaagaa
     4441 atatgttttt ttaataggtg aagttcctga gcaatatgat acaaatggat gtttgcacct
     4501 tttaatgaga ttggatagat ctttgtcttt tcagtttcta atccattttg atagctagca
     4561 gcatcgcaca aaaccactct ttttttcgca catagacatt catcaggcaa ttcttctaat
     4621 gaacgaaata aaataggatt tatttcagcg atgccttctt ccgcatacaa atgaaattgg
     4681 gttcgataaa aaaattgttt gactgaactt tctgaaacaa caaaaatttc tatttcctgt
     4741 ctccccgctt gttcactgtt gatcaaagaa agacttttga cgaattttcg aatgaaattt
     4801 tcattccatc ttagttggat ttgatttttc tttttccact cttccaatat ttctacaatt
     4861 tttccgtaca gaagttgttc tttttctgat agaagataga tcctttccgg taaaaatact
     4921 tgtccatctg cccacaacgt acggacaaat tgtaaaaagg attgcttgaa aataaaattt
     4981 tctgcatcgt cgatttcagc gatactaata atctgtaaaa tcaattcatt cagtgatagg
     5041 tcattgagaa caaaattttt ataaacaact tcgaaatctt ttcttaacag ctccatgttc
     5101 tcattggtat aatttctact gttgaacaaa gaaaaaatat aatagacatc ttcttcttgt
     5161 aagtctagaa ttcctttttg ctcttccaat ccttgacaga ctaattgaaa taacggcttc
     5221 gtttctgcgc ttttcttatc ttcttctttc agtccaaggg gaaatgcctt tcttccgatt
     5281 gcgatctcta ttcctcgtat gataaaggac ttttcatctt ccgaatatct tcttttaaaa
     5341 aaatgagtct ctacataatc aatcaactcg ttacaagctt cttctacata cggattttta
     5401 ttttcatagc cttcccaatt gacataacgt actaaagcta gcaagatgtt ccgcgagtca
     5461 aattctggga taatgatttc tttatttttc aaataatcat tttctttaaa aaaatccaat
     5521 acgatttttt tgattgtata tactttgctc aaagaaataa attcaagatc agccagttct
     5581 gtactcgtga atataccatt aaaataataa cttaaaaact ttaaaaaata agattgaccg
     5641 tacagaaact gtgtgagctc agaaagagca ataccatgtt tgaatacgag tttatactta
     5701 tgcttctctc ttacttgttc ttttatacaa tattctagac actcggctag actttctaga
     5761 tcgtgaagga tcgttggtgt agtcaccctt agagaaatag ctgtttgttc gatgtctatt
     5821 tcttggaatt caaataattg ttcacacaga tacacttttc taaatatatt tcgctcgatg
     5881 taattttcca gcattcctga tacacgcctc cagtttttct tatgatttta tttaatcata
     5941 tctgtaaatc gtatttttgt ttccttttta tttagaaaag ccctttcaaa aaccaataaa
     6001 aaaagaaact gggacaaaag tcaaacaagc tcacaatttc agcctcattg taggtagctt
     6061 tgagccgttt ggacttttac cccagcctct ttcaagaatg gttattcaaa gatgtcagcg
     6121 tctagttctt tgactacatc tttcaacaat tggataccag ctgcgctgtt accaaatgga
     6181 tctagtgccg ggctgaagac acctatgccg taacgatttg gcacagctgc catcaacccg
     6241 ccgcctacac cgctttttgc aggtacacca acatggacgg aaaactctcc tgattcatca
     6301 tacaaaccag cagttgtcat aattgatttg acgattgtag cactttcttc agtaatgatt
     6361 tgtttgccat tccaaggagc aattccttta tttgccagca cagcagctaa ttttgctatg
     6421 cctgtagcgg tgacattgag ggagcattgt ctgaaataag catcgagtat ctccggtacg
     6481 tctccttcga tcatttgatt acctttcata tagtaagcaa gagagcgatt gatatcacct
     6541 gttcttgatt cagaatgata gatttcttca ttcaatgtga tattaggatc atcacagatc
     6601 tctttcataa atgacaagat tcgctcaaat ttttcatctg catctttacc ggcaatcaac
     6661 gaggttgttg caatcgctcc tgcattaaca aaaggattca ttggatgttt gcgatgattg
     6721 atttccatat tcatgataga attgaaggca aaaccggttg gttccgtgtt gattgtttga
     6781 aagacttttt ctattccgtt gtctttgatt gctaataaca atacgggaac ttttgacatg
     6841 ctttcgatgg caaagcgcac atcggcgtct cctccctcaa tcagttgttt tgtatccaaa
     6901 tcataaatcg ccatacctaa ttgtttggga tccacttcag ctaatgctgg aatgtaatcc
     6961 gccacttgtc ctgtttgata atagtggaaa ttttttccaa tcaattgttc taattctttt
     7021 acttccattt gaataaccag ctttcatttt ttatttttct gcatgctgga atttatgacg
     7081 gcatgcgtag atcgtaaatg gaataactaa gacgataaga taagaaacaa tcagaatcgt
     7141 ttcataaatc ccatgctgtc cagctgcgat actagcaggt ggaacaaatg agatacaaaa
     7201 agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt acttttacaa cttttccgcc
     7261 tggaacctga taggctcttt caagactgtc atctttaaaa accagcttca aatagcccgc
     7321 gaaaaacata aagtacaaca tcaggtagat tacaacagtc aatgacatcg ctgcaagaaa
     7381 tgaaagatta ttccccccgc cgcttaatgt aaagacggct gcccagattg ttgcaacgat
     7441 cccatttgcg atcatcaaat gagcaggaac accgtgtgtg ttttcttttg caaaatattc
     7501 aggaagtaat ccttcttctg ctgctacgtg aagtgctttt gaaggagagg ttacccaaga
     7561 agcaatttct ccgatgacac ctaaacaaac aagaatcgca agaattttcg caatccatgc
     7621 aagatttgga tcaatgtgtc ttaataggaa ttctaacgat tgaatcaccc ctgtattaag
     7681 agacaaacca tgttgaggta ctgtagtagc gacggtcaat ccgccaaaag tatctaaaat
     7741 gattgccaga ataaccaata caaacattgc taatggataa tcctttttag ggttttccat
     7801 ttcatttgca tgactggcag atgtttctac gcccatatat gctaaaataa aggaaacaaa
     7861 aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat gttttcatag acagtatcgt
     7921 ttgtagtgga gcgcctgaag cgacataatg gatccctaag ccaaacaaga ctaatgctgg
     7981 caacaagata ccagctacaa aaccgatccg agcaatcaat gaagtgtatt tggttccttt
     8041 taactgtgag agtaaaatag cccagaaaat aatcaatagg attgccaatt tcagatattt
     8101 attgttttgt atagcaccaa tacccgttgc ataggataat gcaccagtta agaaatacag
     8161 catggtcaag tagccaactg ttatctcaaa ccattggaaa aagattgcgg caaaaccgaa
     8221 tcgttttccc aatgtgcgac tgacccaagt atagacaccg cctttttccc atcctttcac
     8281 cgttgccatc tctgctgcgc acaatgccac agggataaac catagcaaac cgcctaataa
     8341 caagaaaaat actaagctga agcctgaggt cgcgaatgtg ggatattcat ataatgaaac
     8401 gaccattgag atggtcatag ccaaaaagcc aaagaacgtt aatttttttg caggaatttt
     8461 cgttttgctt tgttgcatga atcttcctct tttctatttg aatttgtttc aattgagttt
     8521 gttgccaaag aagaaaaagt ccaatccttc ttcttgttct tcttttgtag tagtgatcgt
     8581 tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc acataggcat acacggcagt
     8641 aatgatgaca ccaatcactt gaatccctaa gaagtaccag ctgcttggat tcgttagtaa
     8701 atggctgtta gatgcaaaga tccctatcag aatcgtacct gtgaatccgc ccatgccatg
     8761 aacaccccac acatcaagtg catcatccca ttgtttcttt ttacgaaaat caacagcagc
     8821 atgacagacg atacccgcaa tgatcccaat cagcatggca ctttttgctt ctacataacc
     8881 ggcacaaggc gtaatggctg ccaatccagc aacggatcct gttagtacgt cgacaaaatc
     8941 aaagccattt ccttttactt ttgcaatgat cagccaagta atcatcgcaa atgcaagagc
     9001 gatgaatgtt gaaccaaatg cattgactgc ttgttcatct gctcgaagtg caccaccgga
     9061 attaaaaccg aaccagccaa accaaagcag tcctgtaccg atagcagctg ccataagact
     9121 gctatgctga cttctttctc ctaaaggaat ctttctttga ccaagtgtga ggatacatgc
     9181 taaagagccg aatccagctg tcgtgtggat gactgctcca ccagcgaaat ctctaaatcc
     9241 catctgatcc aaaaatccgc caccccatat ccagtggcaa acagggaaat aaatcagcag
     9301 attccaaaaa ataacaagta taatatagcc ttttaaattc aatctccctg caaaagctcc
     9361 tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg taaagaaaga acattaaaaa
     9421 tggaatcgtt gcaccatgaa acatgttggg gaagcttgta acatgtctca tcaaaaagaa
     9481 atctgccggg tttccaataa caccgccgat atcccgtcca aaggccaatc cgaatccacc
     9541 aaatacccac ataatcgttg ttatcccaac ggagagaaaa gattgagaca tgatcgttag
     9601 tatattattc ttccgtgcta atccgccgta aaaaaaagct agaccaggag tcatcaggca
     9661 aaccattgct gtacataaaa tcatgaatgc tgtatctcct gtattgatag gtaatgtatt
     9721 catttttttc gcctcctttc tacgattacg agtataaaag aaggtgtagt tgtttttttc
     9781 ttctgttttt ttgaattaat acttgcattt ataaaagaca gacaattaat aaaagcttga
     9841 tgtgttgtgc tctttttcgt ttttcacaca aataaagaca aaaaataaac ggtcttcttt
     9901 ttatagaaaa ccgtttattt tc
//
LOCUS       DACDID010000098         9583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDID010000098 DACDID010000000
VERSION     DACDID010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.099"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..636
                     /locus_tag="IV236_001947"
     CDS             13..636
                     /locus_tag="IV236_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772129.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /locus_tag="IV236_001948"
     CDS             1086..2135
                     /locus_tag="IV236_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8772130.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="IV236_001949"
     CDS             2132..3013
                     /locus_tag="IV236_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8772131.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="IV236_001950"
     CDS             3016..3786
                     /locus_tag="IV236_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8772132.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="IV236_001951"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="IV236_001951"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8772133.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="IV236_001952"
     CDS             5097..6260
                     /locus_tag="IV236_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8772134.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="IV236_001953"
     CDS             6377..6787
                     /locus_tag="IV236_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8772135.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="IV236_001954"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="IV236_001954"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8772136.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9327..>9583
                     /locus_tag="IV236_001955"
     CDS             9327..>9583
                     /locus_tag="IV236_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772137.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAA"
BASE COUNT         3322 a         1631 c         2070 g         2560 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggc
//
LOCUS       DACDID010000099         9534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDID010000099 DACDID010000000
VERSION     DACDID010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.100"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            222..1457
                     /locus_tag="IV236_001956"
     CDS             222..1457
                     /locus_tag="IV236_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8772138.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2456..2716)
                     /locus_tag="IV236_001957"
     CDS             complement(2456..2716)
                     /locus_tag="IV236_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8772139.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2743..4044)
                     /locus_tag="IV236_001958"
     CDS             complement(2743..4044)
                     /locus_tag="IV236_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772140.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4336..4794)
                     /locus_tag="IV236_001959"
     CDS             complement(4336..4794)
                     /locus_tag="IV236_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8772141.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4807..6132)
                     /locus_tag="IV236_001960"
     CDS             complement(4807..6132)
                     /locus_tag="IV236_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8772142.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6149..7561)
                     /locus_tag="IV236_001961"
     CDS             complement(6149..7561)
                     /locus_tag="IV236_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772143.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7578..8342)
                     /locus_tag="IV236_001962"
     CDS             complement(7578..8342)
                     /locus_tag="IV236_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8772144.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8446..9321)
                     /locus_tag="IV236_001963"
     CDS             complement(8446..9321)
                     /locus_tag="IV236_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772145.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3096 a         1982 c         1442 g         3014 t
ORIGIN      
        1 ctcgtattta tctggcacaa catataaatc gatcaaaaaa ttgttgcgtg tttttttgaa
       61 aattggagtt tcaacttgat tacaaaattt aaatttagaa gttgtgaaaa cagcgagaga
      121 cagtttaata ttcaaaatat acataggcta ctcgaacgct gtgtttttta gtatactagt
      181 aagaaacgaa gggatagact cattccaata aggaggattt catgaagaca attctttttg
      241 ctcctgcagt attcaattta gctgagacaa cacgaatgat tgaggttgcc aaagctatga
      301 aagaccgtta ttactgtgta ttctttggct tttctaaaac ttttgctcct ttgatcacag
      361 aaagtggctt tgagtatcat ttactagcgc cgatccttac gaaaaaacaa gaagaacaaa
      421 tcataaaatt tgaccagttg cgtacactta aaaacccttt taccaaagaa atggttgcct
      481 ctcgtatcac tagtgaacag agtctgattg ctaagtttga gcctcgactg attgtgacag
      541 gttcgaatgt gacaattttt ttatcggccc gcaataaaaa gattccttta gtatatgtta
      601 aaccctatgc tttgagtgaa cctttttttc aatcagagca tgcgctgatg ccccacgagt
      661 tacgtttctt cggcccttta agcaatcccc tttggcactg gacaaaaaag cgtatagtaa
      721 atacccgatg gatccccaat gccttcaaac acgctgcaca aaaagaaatg cttcctttac
      781 caaaaacaag tttacaaatg atggatgctg atctaaatct catcacaacg cctaaactat
      841 ttttggaaga agaacagctg ccggaaaatt atcgaatcgt tggtccaatt tttgctaagc
      901 tcagtacttc cttgcctgaa gaagtgctaa cttttataca gcgaaagcaa caagaaagaa
      961 aacgtattat ctatttcgca atgggtagtt caggcaatcc gcgtatgtta cgcaaaattt
     1021 tgttcttctt acaaaaacaa ccggatctag cggttattgc acctattgac tatctgcttg
     1081 atgacattca atctgactca gaagatcttt taataacaga atatctgtct agtcatcttt
     1141 tgcatgaatt cattgatttt tcttttatcc acggcgggga agggaccgtc caaactgcct
     1201 gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt tgagcaaagc tgtaatattg
     1261 actattgtgt aaaatacgga aacgcaatcg ccctcacaaa accagtgact gaaagaaaac
     1321 tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg tcagcaggca cgcacgctgc
     1381 aagccgtttt tccaataaat ggtccacaac aagcaattca agaaatcgaa accttcctga
     1441 gcaaccaccc aatatagttt caagtagaaa aagctttttt aatagagact catcagtaat
     1501 ttcgctattc gagacccaga cagtgaaaat acaaaaaaag taaatagtgg attgaaaagt
     1561 tagagttact taaattacgg tgtctaaaaa acacaaaatc atccttaaaa taaaagatct
     1621 gacgctatcc aaatgagcat aggtagcgtc tgccatttat tcttagtcaa attctttgac
     1681 acctttctct ggttggatca tctcaacgtt tcttcaaaag aaagaccata ataattaggc
     1741 aaatacattt tcttaaaatg tgtcatatag atctagaagc atcgctttgt agagatttgt
     1801 gccgctagtc ccctcaacaa ttactaaatc aaatgtagga tttaccactt tatctttgat
     1861 aatcaacctc cctcttctgt tgagacaatg atctcgcaat ccgatgcata ctcctatata
     1921 aactgccagc aatccccaca tattgagtag atcatgtcag aatgttctat gtcgtcagca
     1981 acgatacact aaaagtcacg ttcaccatga ctgattgcta ttcccagcac aattatttct
     2041 atacacgttt catgatttga ataaacactc acatcataca tattaccatt catacaccat
     2101 actgcagccc ctactatatg gttgtaagct cccaaatcta tatttgactt gatcactttg
     2161 gtagtagatc tactaattct aaatccattg ctgataaaat ttccattttt tcctccttct
     2221 ttctgccaaa acagttaaaa caataataat ctttatgtaa aaaagggctg tgacaaaagc
     2281 cttgtcacag tcccatattc cgaataaacg gtggtcaaaa gcttgcatct gcggtaataa
     2341 gttcaaccaa acgaaaaata agccaacatt tcacaaaaat ttaagaagca attttcgtaa
     2401 atttcttctt atttcttgaa gctaaccact tctgttacaa ccacttttag aacatttaaa
     2461 gcttcgatgt gacaaaccat ctatgctgct cgatcataaa attgatagca aaaattccac
     2521 aaagtacata tgtcccccat gaaaacaaaa cattcatatg cggtacaatg atctttggaa
     2581 gaatcacgat caaaaatgat gccagtgccc aaatccatcg attgatttga atatgatatg
     2641 tagctagcat attcaatata aggtgactag ccgttaataa aaagataaaa acaaaaaata
     2701 aaatcagcca atacattgtt tcccaccttc taaaaaccaa acttagacag tctggacttc
     2761 gttttctcca cgactttgtt catctgacag catcttttta tcataagctt ttacaaatgg
     2821 ataatagata ataaatgcta tcaatgtgtt gatcatgatc aagataacaa cacgccagtc
     2881 atttcctgct gaaagataac cgccgattgg agctggtaat gtccatgttg gcaaaacagt
     2941 aaatctattg accatttcta atttgactgc aaaataactg ataatagtca aaactaaagg
     3001 aactaagttg aacgggatga acatgtaagg attcaacatc ataggagctc caaaaattat
     3061 gggctcattg atattgaaga taccaggaat aatagaaaaa cgaccaacct ctttcaagaa
     3121 ttttgattta cagaagaata taaacaaaac agataccacg atcgttgctc ctgcaccacc
     3181 aattgttacc gtccattgat aaaactgttc aggagcgata tagggtaatt gggtggcagc
     3241 tgtaccatct gccaatgcct ttgcattctc atccaacatg atcagccaca ttggacgaac
     3301 aacagcccct acaatactca tgccatggat accaaatgac cacaacaaat ggatcaaaaa
     3361 cattggtagt aaaacaccaa gcaaattatt ccctaaaata ttggttaaag gctcgaataa
     3421 cgataataag gcagcgttta catcaaagcc cgcgacgctt cgaatcagcc aagtaattgt
     3481 taaaatgact gcacctggaa tcaatgcctc aaaactacgg gcaacagcag gtggcacttg
     3541 ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa cgatatactt ccactgcaaa
     3601 aatcatagat aagatccccg caaacatccc agatgctccc aaagaactca taggtaagac
     3661 ccagccaaga ggtgcctctt caggagttaa aacgtttaca ggaacttgta acataaagaa
     3721 cgttcctaaa gataatatac cacctgtcac actatctaat ccatatgatt tagctagatc
     3781 cgaacccata acaaaagttg cataaatagc catcaatccc atcgtcatcc taaagggtaa
     3841 aacaatcgtt gccgaataag gggcaataaa ttcattccat ccaggaaacg gtaaattgat
     3901 aaagaccata aaaaatgtcc caatcaaaat caatgacaat gttgaaacaa ccccattacg
     3961 aattgctaat aaatgacgct gtgtaccaat ttttgccatt ggtgggacaa gtttctcttc
     4021 gacccatttc atcagctttt ccactaaaac cactcctttt caataaaata tgaaccattc
     4081 attgcatgac aacgcaaaga atgttcataa ctaattatat aattaagaaa ttttatttca
     4141 atatattctc ttaaataatc aaatttattt ttaagaatcc tgaaaataag ttttctaaat
     4201 cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa atctagtgat
     4261 tgaaacatat catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac
     4321 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
     4381 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
     4441 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
     4501 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
     4561 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
     4621 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
     4681 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
     4741 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
     4801 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
     4861 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
     4921 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
     4981 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
     5041 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
     5101 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
     5161 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
     5221 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
     5281 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     5341 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     5401 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     5461 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     5521 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     5581 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     5641 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     5701 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     5761 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     5821 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     5881 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     5941 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     6001 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     6061 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     6121 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     6181 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     6241 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     6301 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     6361 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     6421 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     6481 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     6541 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     6601 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     6661 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     6721 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     6781 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     6841 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     6901 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     6961 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     7021 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     7081 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     7141 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     7201 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     7261 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     7321 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     7381 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     7441 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     7501 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     7561 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     7621 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     7681 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     7741 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     7801 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     7861 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     7921 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     7981 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     8041 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     8101 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     8161 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     8221 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     8281 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     8341 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     8401 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     8461 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     8521 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     8581 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     8641 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     8701 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     8761 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     8821 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     8881 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     8941 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     9001 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     9061 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     9121 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     9181 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     9241 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     9301 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     9361 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     9421 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     9481 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaa
//
LOCUS       DACDID010000100         9526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDID010000100 DACDID010000000
VERSION     DACDID010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.101"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(303..1352)
                     /gene="recA"
                     /locus_tag="IV236_001964"
     CDS             complement(303..1352)
                     /gene="recA"
                     /locus_tag="IV236_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8772146.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1461..2702)
                     /locus_tag="IV236_001965"
     CDS             complement(1461..2702)
                     /locus_tag="IV236_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8772147.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2942..3520)
                     /gene="pgsA"
                     /locus_tag="IV236_001966"
     CDS             complement(2942..3520)
                     /gene="pgsA"
                     /locus_tag="IV236_001966"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8772148.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3729..4613)
                     /locus_tag="IV236_001967"
     CDS             complement(3729..4613)
                     /locus_tag="IV236_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772149.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(5391..6677)
                     /locus_tag="IV236_001968"
     CDS             complement(5391..6677)
                     /locus_tag="IV236_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8772150.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6670..7938)
                     /locus_tag="IV236_001969"
     CDS             complement(6670..7938)
                     /locus_tag="IV236_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8772151.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8190..8651
                     /gene="mscL"
                     /locus_tag="IV236_001970"
     CDS             8190..8651
                     /gene="mscL"
                     /locus_tag="IV236_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8772152.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8711..9157)
                     /locus_tag="IV236_001971"
     CDS             complement(8711..9157)
                     /locus_tag="IV236_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772153.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9250..9495)
                     /locus_tag="IV236_001972"
     CDS             complement(9250..9495)
                     /locus_tag="IV236_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772154.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2643 a         2055 c         1680 g         3148 t
ORIGIN      
        1 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
       61 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
      121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
      181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
      241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
      301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
      361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
      421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
      481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
      541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
      601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
      661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
      721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
      781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
      841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
      901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
      961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
     1021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
     1081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
     1141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
     1201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
     1261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
     1321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
     1381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
     1441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
     1501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
     1561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
     1621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
     1681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
     1741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
     1801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
     1861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
     1921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
     1981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
     2041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
     2101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
     2161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
     2221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
     2281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
     2341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
     2401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
     2461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
     2521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
     2581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
     2641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
     2701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
     2761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
     2821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
     2881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
     2941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
     3001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
     3061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
     3121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
     3181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
     3241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
     3301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
     3361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
     3421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
     3481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
     3541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
     3601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
     3661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
     3721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
     3781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
     3841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
     3901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
     3961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
     4021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
     4081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
     4141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
     4201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
     4261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
     4321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
     4381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
     4441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
     4501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
     4561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
     4621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
     4681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
     4741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
     4801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
     4861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
     4921 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
     4981 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
     5041 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
     5101 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
     5161 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
     5221 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
     5281 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
     5341 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
     5401 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
     5461 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
     5521 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
     5581 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
     5641 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
     5701 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
     5761 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
     5821 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
     5881 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
     5941 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
     6001 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
     6061 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
     6121 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
     6181 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
     6241 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
     6301 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
     6361 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
     6421 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
     6481 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
     6541 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
     6601 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
     6661 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
     6721 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
     6781 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
     6841 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
     6901 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
     6961 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
     7021 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
     7081 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
     7141 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
     7201 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
     7261 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
     7321 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
     7381 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
     7441 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
     7501 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
     7561 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
     7621 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
     7681 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
     7741 ctctgcaagt tgcgcactta actttgtctg atccgaataa ttcaaactat ttgtttccaa
     7801 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
     7861 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
     7921 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
     7981 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
     8041 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
     8101 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
     8161 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
     8221 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
     8281 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
     8341 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
     8401 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
     8461 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
     8521 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
     8581 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
     8641 cttctaaata ataaagaatc taaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
     8701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
     8761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
     8821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
     8881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
     8941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
     9001 tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac
     9061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
     9121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
     9181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
     9241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
     9301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
     9361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
     9421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
     9481 taaagttgtt ggcatacagt tcacctctac tatcaactat accaca
//
LOCUS       DACDID010000101         9488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDID010000101 DACDID010000000
VERSION     DACDID010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.102"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            179..1060
                     /locus_tag="IV236_001973"
     CDS             179..1060
                     /locus_tag="IV236_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772155.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1073..2683
                     /locus_tag="IV236_001974"
     CDS             1073..2683
                     /locus_tag="IV236_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8772156.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2760..3419)
                     /locus_tag="IV236_001975"
     CDS             complement(2760..3419)
                     /locus_tag="IV236_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8772157.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3702..4367)
                     /locus_tag="IV236_001976"
     CDS             complement(3702..4367)
                     /locus_tag="IV236_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772158.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4514..5119)
                     /gene="dhaL"
                     /locus_tag="IV236_001977"
     CDS             complement(4514..5119)
                     /gene="dhaL"
                     /locus_tag="IV236_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8772159.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5147..6130)
                     /gene="dhaK"
                     /locus_tag="IV236_001978"
     CDS             complement(5147..6130)
                     /gene="dhaK"
                     /locus_tag="IV236_001978"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8772160.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6132..6527)
                     /gene="dhaM"
                     /locus_tag="IV236_001979"
     CDS             complement(6132..6527)
                     /gene="dhaM"
                     /locus_tag="IV236_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8772161.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6681..7001
                     /locus_tag="IV236_001980"
     CDS             6681..7001
                     /locus_tag="IV236_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8772162.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7113..7982)
                     /locus_tag="IV236_001981"
     CDS             complement(7113..7982)
                     /locus_tag="IV236_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8772163.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8078..9352)
                     /locus_tag="IV236_001982"
     CDS             complement(8078..9352)
                     /locus_tag="IV236_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8772164.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         2553 a         1977 c         1787 g         3171 t
ORIGIN      
        1 ctattgagaa agtatcaagc tagcaattcc ttgtttatga attcattgac agaaatttat
       61 aaaaagaata ttatgtacta aaataataaa aaaagtttta taaaaaggta taaaaccttt
      121 ttattaaact atttaatctt gtttagtata tatttttaga aaaaggagtg gggtttttat
      181 ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa
      241 agatgtctct ttcaccgtac attcaggtga agtcgttgga tttatcggac caaatggttc
      301 tggaaaatcc acgacgattc ggacactttt agggattata aaaaaagacg gcggaacggc
      361 aacgattttt gggcaagatg tatgggaaga tagtttatct atccatgaac gtctttctta
      421 tgttccaggc gatatctctt tgtggggaaa tttgactgga ggagcgatca tcgatctctt
      481 catgaagctt catggaaaag ggagtgctca aaaaaaagat gaactgattc aacgtttctc
      541 tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt
      601 gattgctgct ttggctgttg attcggatct ctatttattt gatgagccta cttctggttt
      661 agatcctttg atggaaacga ttttccaaga agaagttgaa aaattgaaag aacagggcaa
      721 atccatccta ctttcttctc atattctcag tgaagtcgag cgactagcgg ataaagtcgt
      781 gatcatacgc gaaggaacag tcgtagaaga aggaacactc gatgaattgc gtcatttgac
      841 tcgttcgacc atcactatag aaacagaagc agagaatagt cacttagcac aattgaacgg
      901 tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt gctgctgata acgaagcttt
      961 cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc
     1021 gactcttgag gatttattca tgagacatta tgaaggatga ggaggaagcg aaatgactga
     1081 taaattttca cgctggtcca cccttttttc tcaaaacctt agccgtgact ggaaaaagat
     1141 cctgatatgg ctagtcggtg tcactgcttt ttctggtggt tttgtccctg cgtttgaaga
     1201 attgacttcc ggacaaggat tagctggatt atacgaaacc ttgcaaaacc cggcaatgat
     1261 tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag tatactttag gagcgatgta
     1321 tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct gtgatctctg ttttacacgt
     1381 ggtgagccaa acgagaaaaa aagaagaact tggtctgatt gaacttattc gttcctttcg
     1441 agtcggacgt caagcaaact ctttagctgt catcgctgaa agtattctga tcaatttgct
     1501 tttagcaata tcgatcagct gtacgatgat ttttttccaa gcagattcga tcactgcaga
     1561 aggtgcttgg ctttttgggc tatctgtagg tagtatgggg attttgggga gtgccgttgc
     1621 actgtttgct gcacaactga tgccaagttc agccagttcg aatggtctgt ctttagcgtt
     1681 tttaggtgga ctttaccttc tacgagcaat aacagatgta gctgattcct ccctttcgat
     1741 gtggaatcct ttaggctggt cttatttgac ctatccattt acaactaaca actggattcc
     1801 tttgctttat ttgtttttgt tcagtctaat cttgataatc agttcatttg ttttagagga
     1861 acatcgagat ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg
     1921 ttctctttta tccttgcgtg gtttattatt ctatctaaat aaagggatga caatcagttg
     1981 gcttatcact tttggtttga tgggggcggc atacggctct atctacggga atatgcaaca
     2041 gtttttagaa agtaacgact taatgaaaca gatgtttatc caatctggcg tctcaattga
     2101 aaaaaacttt acaagcacga tcatggtcgt cttgatttgt ctagctgcta ttttaccaat
     2161 tgctagcatc aatcgtttat ttacagaaga aacccacgga cgtatgaatc agctgttcgc
     2221 aacaaaaatt tctaggaacc aactttattg gaccaatatg ttactagcta ttctatttgc
     2281 tatattggca atcctgatta gttctggtgc actagggctt tctgctttgg cttcgatgaa
     2341 ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc
     2401 cgttttagtg tttatcggaa ttagtggctt tctattcgga tggcttcctc gaatgggtaa
     2461 acttgcttat atctacttag cttactcctt tgcactcaat tactttggcg gaattttgga
     2521 tttaccagaa tggttttcca aaacagctgt ccctagctgg gttccacgga tgccaaacga
     2581 ttcttttgat ctttccgtgt tccttacgtt atcatgtatc agccttgcac tgttcgtcct
     2641 tggttctatt ggttataggc gaagagactt actagaagga taataaaaaa gcatgtacaa
     2701 tttcatgcta gcctccaaat ctgggtcaaa aatccaacat ttggaggtca tttttttatt
     2761 tacaagtctt ctaactgctc tttatcaatg atccagactt ctcgattctt tctttttaca
     2821 taaccattct cctctaataa tttaaatttt ctagataaag tttcgggggt cgtaccgata
     2881 aatgcagcaa gttctttcat tttcattggc aggaaaaagt gatccttccc tgtcgcttta
     2941 gctaagtcaa gcagatagac agcgactcgt tcttctactt tttccatcat caaaaaagtt
     3001 gtctgctgct gtacttttgt taatttctca acgttgattg ctaaaagttt cagactaagg
     3061 gcgggataat gcagtaagag ttccgagaaa tctttttgtc tcaatatgca aatttccgtt
     3121 ttttctagtg cttcgccaaa taaagactca tttggaacgc ctagcaactg gctctctccc
     3181 tcatatccgc ccggctctat tactcgtaaa agctgttcct ttccgctagc agataattga
     3241 tacactttca tactgccgga ggccacgatc actaattgag gttctccatt cggcgcaaga
     3301 atctgttctc ctttttccac atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct
     3361 tgctgtacta agtgattgaa caaaggaaca aatgaaacac ataaatgatc atgactcata
     3421 atctctcctc cttctaaaca gctatttcta ataaagaaac ataacttccc ttattccaac
     3481 tgataatttt cttttatcat agcataaacg tcaagcgaac cagataccag caaatagagt
     3541 ggatttcttt ttgtcccttg ctttattttc cttcttttga gaccttaagt aattgattcc
     3601 tatcaatttt ttaaatattt actttttgaa taatcaaaaa caaaccttca ctcgcaagcc
     3661 gagagtaagg tttgtttttc gcattgctta tttcgtgttg tctattgaaa tttttccgca
     3721 cctagctttt gatatagtgg tgctagttct tgtaaggttt cagcggttat ttgctcaacg
     3781 tgttcgattt cttgcggata gaagaatagt ttacccattt gaaaggttcg gtctctcatc
     3841 gcttttttca actcttcttg tttctttgta tcggacaaat agtcagacaa agctaagtgg
     3901 tcgtcaagtt catgttctgg ttgcggccat atgtaataat ccttgatatc gactgttttg
     3961 ctccactctt tcagtatcgc aagcaactgg ttatacgctg caatgctacc atatcgttct
     4021 tcgcttttat aatgttggta catcaaatag atttggtatt gtttttgatt caacaaaact
     4081 gctagacagg cattctcgtc ttgacgagat tcgcttcggt acgcgctcca aaaatgactg
     4141 cgtaaattcc agccattcgt ccagctttcg attttaggtt tgctcatgcc gtacatatca
     4201 ggcaaaagtg cagctgtctg gagctgaatc tctttccatt tttgccagtg ttctttatat
     4261 tctttcttga tttgttcaat cttatctgtt ggatggctct ctttgagctg tttgaatgcg
     4321 tagattggtc gatcaaacaa ctgataatct gattttccaa gttccattgt ttcgcctctt
     4381 tccgttatct tttgtattct acctgaattt tgcgatatgg acaaatagag gttgtgaaaa
     4441 agctgttctg catcaagtca taagaacagc caaacaaaaa actgcgatga gcatcaccgc
     4501 agttttattg aacttaaggt tgattgatca catctaaaag cgttgaaaat aaaatggcac
     4561 tagaagcagc tcctgggtcg atatgtccga ttgcacgctc tcctagatag gaagctcgtc
     4621 cttttttagc taccaagact ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta
     4681 aattccctgc cttcaatgcc tctgtgactg gcagccaaac atctaacatc gttttatcgt
     4741 cagcttctgc ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc acttcgccta
     4801 atttttcttg agaatcaata gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg
     4861 cagatccgta taacggacca gaagccccac cgactttcga tatcatcgcc atagagaata
     4921 ctttgaatgt atcgctgatt gtttcaggat gatcggcttg aaaagcttct tcataagctg
     4981 aaacccctct tcccatgtta tttccatggt ctccatcgcc aatcggggta tctagatcac
     5041 tgagatattg tttgttctga ttgattgcct ctgcaaattg cgtgagccat tcttggattt
     5101 cttgaacggt tagtttcata ttatcaccct tttatcttga gattttttac catgaaatcg
     5161 tctgtacatt gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta
     5221 gagaaagacc ttgcatatct agtgaggtca taaaattacc gacttttcgg aaaactacat
     5281 tgacgcctct ttcttccaac agagctaaaa catcattcat gaacacgaac tgttccatca
     5341 aaggcgtccc gcccattccg ttgactagta cacctgcttc ttttggtaaa ttcgagtaat
     5401 catctaaaat cttagtaaca agttcctttg ccagtacttt agaaggctgc atcttttctc
     5461 gacgatagcc tggctcaccg tggataccaa caccatactc gatctcatct tctcctagtt
     5521 cgaatcctgg tttccctact tctggtacag ttgctgctcg aagcgctaga ccgatcgtct
     5581 tagttgcctg tacgaccttt tctcccagtt ctttcaactc agctagggaa gcaccgtttc
     5641 ttgctgcatc gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg cgttttccag
     5701 cagtatacgt actattttca acggcgatat catcatctac gatcacactt tctacttcaa
     5761 tatcgtccat agcagctaat tcctttgcca tatcgaagtt caatgcatcc cctgtataat
     5821 tcttaacgat cagcaaaact ccttttccac tatccgcttc cttaatagcc gtctgaatct
     5881 gatctggagt tggagaagta aacacatcac caagcaccgc tgcacttaac atcccttccc
     5941 ctacaaaccc ggcatgggat ggttcatgtc cgcttcctcc accagaaacc aaaccaactt
     6001 gttccgcttt ctgattcttg gctacaacac gggaagattc tacacgatga acaagttctg
     6061 gatagctttt tactaatcct tccagcattt cttcgacaat attgcttgga tcattcatga
     6121 tttttttcaa tttattccca tcctttcttt ccggcatttt gtgcttctgt taagatttgc
     6181 tgagtatcat ctgttatacc ggcagtgatg gctgctgcaa aagctccttc cactaaaggc
     6241 gcatccacca gatgataacg ttgctgctgc tcttcttcca acatgtcttt tgccagttcc
     6301 gagttcaata ccgcgctacc gatatccgca aaaacaaaat aactagctgc atcgcttgat
     6361 tcattgactg cttctacgat tttcatcggg tctgatccga tctcgccatt tattccgccg
     6421 agagaaaaaa tttgcacctc ttcggatttt tgcatctgct caatcatttc tttaatacca
     6481 tctgttactt tttgactatg agaaataagc aaaatacttt ttttcacaag aatcttcctt
     6541 tcttttacat agttgctaac gattggtttc gctttcatta atgaacaatt caagtatata
     6601 ataaatttct aattttggga agatatgtgt agtgcttctt gaagtttgtt atactttaat
     6661 taaaagagag gagtgatcga atgattacaa caacaacgaa cagtgtagag ggaaaagccg
     6721 tagtagctta taaagggatc gtttttggtg aggtcattac tgggatccat gcatttaaag
     6781 acttggaggc ggggttgcga aatatctttg gcggccgctc aaaaagctat gaaaacgaac
     6841 taatgggtgc aagagaagaa gcacttgccg aaatggccga acgtgcaaaa gcattaggag
     6901 ctgatgcgat cattggcgta aaaatggact atgaagtatt aggcagcgac aatggcatgc
     6961 tgatggtcac atgtagtgga acagctgtct ccacgagata agtaggggac atctgtttta
     7021 ataactaaag agagatggga catgtagtgg cactcaagaa ttagattttt cagtctaact
     7081 tcgaggtact ctaccttccc atcctctctt ttttatttct ttttgcggat gattccgtaa
     7141 gctgctgcga ctaacacacc cgcccctgct aaggaataac tgacagaagc tgttttttca
     7201 tttgtttttg gcaaagtctg acttgttgat ttggatggct ctgtcgtttg ctgaactgtt
     7261 tctgtcgtgc ttccagaggt cactgatttt tctcctgccg aatgcaaaat atcttttatt
     7321 cgttggaaac tagctgctaa gtcagctttt tttgcttcaa aatcattagc aactgtttgt
     7381 gcttcgctga tctttccgtg gattgcttga ctggccgctg tgatatccgc ttgagtttga
     7441 cttagctttc cagaagctgt ttgagcaacc tgatttaatt gctgattgaa ttcttttgct
     7501 tgttggatct tcccgtcaag atctgtttgg acagcttccg cttttgcttt cgcttcggca
     7561 agctttttct caagttgtgc acggtcattt cccactttat ttttaagatc agctaaatct
     7621 tgatcgattg ctgcttggtc ttctttcaat ttgttttttg cagcttctaa tgcttcttcg
     7681 agttcttttc ttttattttc caagtcttgc aaatgatcga tttggtcaga agaggtagaa
     7741 ctatttattg atgtatcttc tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca
     7801 ttggaagtca tttctgtttc cgaggcaccg ttttcagcac cagaagagct tatagttgtt
     7861 gaaggttctg tttgagcagg ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa
     7921 atactggaaa cagcaattgg ccagattaac ggcttcattc tttttttcat cacttttccc
     7981 atccttacta ctttttaact aatttcttat tataagcaaa aatgtttgga agaaccataa
     8041 acacaacagg ctgtttgtct aaaaaacggt gcatctttta tttttttgta cgttccgggc
     8101 ttttccatgt cccgcttcca taacctaaaa tcgttttgac tgctggaatg aaagtcgtca
     8161 cgatcgttat cgcgttcact atccagaaca aaagcatata tagtggtgcg aaaagaaggt
     8221 atttcaattt tcttcgtcga tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg
     8281 cgaccatttc aaagcagatg aacacaaaac tgaatgtaaa agtaatataa atctgttcgt
     8341 agtttccttg atatacataa tgcccgatta atccagcaaa aagaactacg gaaatacaaa
     8401 agaatattga ccagacaata ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat
     8461 gtttaaaagg atggagcatc acttttttga aattcgtcag ccagacttct gttccacctt
     8521 ttgcccaacg ttttctttgt cgatatagca tcttcaacgt cacaggtact tccatgaaaa
     8581 agataatctc aggagcaaaa acagataacc agtcatttaa ctgatgatcc caagcaatac
     8641 tgatgtcttc tgtcgctcga tcttgtctga atccgcccac atcgatcaag gcttcttttc
     8701 gatacatcgt atttgctcca ctgtacgcat aaagaccacc gaaaacagct gactgtgttc
     8761 gtttaatgat gcccacgata cttgagaatt caaccgtttg agattttgca atcaatttgg
     8821 tacgattttg gacatccata tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa
     8881 tgaaataatt cacataccta atcagtgcat ctggctccgg taccgtgtct gcatcattgc
     8941 ttaaaatcaa tttccctttt gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt
     9001 tatttttttc gatccgaacc acacgaagat tagcgtattt tttcatcagt ctagctaata
     9061 tttctggtgt ttggtccgtt gaaccatcgt ctgtcactaa tacctcatag ttgtgataat
     9121 tcaatttcgt catcaaatac tcgattgtat cttcgatcac aatttcttca ttatgggctg
     9181 gcaccatgat cgtgatgaac ggttcgacac tcaagggaac atctacccat tctttttgct
     9241 tatgtttgta taaaaacaca taacaccaga caccaataaa ccatacaaat ccgcctaaaa
     9301 tcggataaga aacgagaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
     9361 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
     9421 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
     9481 tcttttgt
//
LOCUS       DACDID010000102         9399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDID010000102 DACDID010000000
VERSION     DACDID010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.103"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="IV236_001983"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="IV236_001983"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8772165.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="IV236_001984"
     CDS             2370..3848
                     /locus_tag="IV236_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8772166.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="IV236_001985"
     CDS             3814..5145
                     /locus_tag="IV236_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8772167.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="IV236_001986"
     CDS             5259..7811
                     /locus_tag="IV236_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8772168.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="IV236_001987"
     CDS             complement(7818..9257)
                     /locus_tag="IV236_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8772169.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2942 a         1569 c         2005 g         2883 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaatt tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc actcttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggc tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccact
//
LOCUS       DACDID010000103         9226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDID010000103 DACDID010000000
VERSION     DACDID010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.104"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(52..1857)
                     /gene="glmS"
                     /locus_tag="IV236_001988"
     CDS             complement(52..1857)
                     /gene="glmS"
                     /locus_tag="IV236_001988"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8772170.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2266..2556
                     /locus_tag="IV236_001989"
     CDS             2266..2556
                     /locus_tag="IV236_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772171.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2569..3594
                     /gene="sppA"
                     /locus_tag="IV236_001990"
     CDS             2569..3594
                     /gene="sppA"
                     /locus_tag="IV236_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8772172.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3597..4256
                     /locus_tag="IV236_001991"
     CDS             3597..4256
                     /locus_tag="IV236_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8772173.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4413..5345
                     /locus_tag="IV236_001992"
     CDS             4413..5345
                     /locus_tag="IV236_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772174.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5499..6788
                     /locus_tag="IV236_001993"
     CDS             5499..6788
                     /locus_tag="IV236_001993"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8772175.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            7035..8285
                     /gene="clpX"
                     /locus_tag="IV236_001994"
     CDS             7035..8285
                     /gene="clpX"
                     /locus_tag="IV236_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8772176.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8368..8955
                     /locus_tag="IV236_001995"
     CDS             8368..8955
                     /locus_tag="IV236_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8772177.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         3040 a         1613 c         1940 g         2633 t
ORIGIN      
        1 aaaagaacaa ggaccagtgt cagatgtctg acactggtcc ttgttctttt attattctac
       61 tgtcactgat ttcgctaaat tacgtggttt gtcaacgtcg aatccccgta atagcgttgc
      121 gtaatatgca agtaattgag tcggtacgac tgtggttaga gtagttagat aagggtggac
      181 agttggtagc accagctggt cactttcctt ttctaatccc tctgagacaa taactagtgt
      241 tttagcgcca cggctttcga cttctttcag gtttcctcga gtatgcgcac cagtcacttc
      301 ttcactgatg attccgatca ctggtgttcc ttcttcgatc aaagcaatcg ttccatgttt
      361 caattcgcca gcggcaaaac cttctgcctg gatataactg atttctttca atttcaaagc
      421 agcttcaaga gaaacgttgt agtcagcacc acgtccaatg taaaaggcat ttcttgtatc
      481 gctgagatat acttctgcca actcttctaa ataatctttt tgggcgatga ccgattccat
      541 cgcattagca actaaactca attcatgagt gatatcgaat acaagagaag tttcaaattc
      601 ttttttatca ccgatagctt tggctaataa agttagtaca gctatttgtg cagtataagc
      661 tttcgttgat gctactgcga tttcgggtcc agcatgcaat agcagtgtaa aggatgcttc
      721 ccgcgaaaga gtagaaccag caacatttgt aatcgtaagt gaagggtagc cgagacggtt
      781 gatttttacc agcacttggc ggctgtctgc tgtttcgcca ctttgactca agaaaataaa
      841 gaatggtttt tttgaaagaa gaggtgtgtt atagccaaat tcactggcta aatgaacctc
      901 aacaggaatc tgtgtcaatt tttccaaagt ctgtttacct gctaaccccg catggtaact
      961 agttccgcaa gcaacgatat agattcgatc gctatcagac aatgtatcga ccagcttttg
     1021 atcaagagag acacgattgt cttctgttac gtatttttga gcgatacgac gcatgataat
     1081 aggctgttcg tcgatttctt tcagcatata aaaaggatag gtacctttct cgatatctga
     1141 agcatctact tgtgcttcga atgaagaacg ttctacgatg tcacctgcaa aattttgaat
     1201 aataactttt tctgatgtga cggtaaccat ctcttcatct tcaatctcaa cgaattcctt
     1261 cgtatgagca agcatagcca ttgcatcgct ggcaatcaca ttgaatccat cgccgagtcc
     1321 gattaatagt ggacttttat ttttagcgac gtaaataaca tcaggtgctg tccgatcaat
     1381 caaagcgaaa gcgtaagaac ctttgatgac ccgcaaagct tttaaaaacg cctcttgcgt
     1441 tgtagtatcc ttcgcgaaag tttcaattag atgagcgata atctcagtat cggtctcacc
     1501 aataaagtgg tcatttgcta aataagcaat tttaagttct tcaaagtttt cgattacgcc
     1561 gttgtgaacg agcacgaagc gtccactttg ggaagtatgt ggatgggcgt tttcttcaga
     1621 aggttgcccg tgagttgccc atcgtgtatg accaatacct atatttcctg tagtatcaat
     1681 atttattttg tttttcaaat tttgaattct gccttgtgct ttgaccagat aatcagttgt
     1741 gccatctgag acaaagatac cagctgaatc ataaccacgg tattctaatt tttctagacc
     1801 gtcaatcaat cctggtgtta cattttttaa tccaatcatt ccaacaattc cgcacataaa
     1861 aaacatccta ctttctataa tgaattcgta tgaatgcttc cagctgcagg aaaatccttt
     1921 tgttttatct ttgcagacat tttttgtgga ttttctgtag agtgcagtcc ctagggccat
     1981 ccgccgaatc tttcgataaa ccctctcctc gtcatctgtt tacacagacc tggcgctatc
     2041 ctaaaaggta taactatcct cctttttgtt ttttatctgg aaacatttaa aatcataacc
     2101 ttttgcgaat gttcttgtca acaaaaaaag aataaaaaat aaattggtat atatcaaatt
     2161 ctttgtcaaa tgtccttttt atgtcaaaaa ataatgggtt tttatctatc atctttttca
     2221 ggagttcggt acaatagaaa gtaatcggat agaggaggag taaaaatgga ttcaaaaatt
     2281 gagatcatga cactgggaat gttaaaaaaa caattgtcca aattcgaagc atcagctggt
     2341 gtatcagatg atacgaaaat atttttagat actggctggg atagcatcca agaaatcgca
     2401 cctgatgcct tggaagttgc tcaagctcgt gagtttacgg tagaagatga attaacaaaa
     2461 gagtcgtttt ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac ttctgagcag
     2521 tcagaaactg tgatcgtcat aaaaaacttg tattagaaag gagaaagaat gaataaaaga
     2581 cgttgggcag cagttctgat tgctgcagga ttatttgtgg tttcgttagt ttcagctagt
     2641 ttgactaagc ctcaggaaaa cgatcaaatt gagggattga acagttggtt atatggggat
     2701 gaagaactag ctccaaatgt gttggaagag ggagacagta cgcaaaaaat cgtaaaatta
     2761 tcagtagatg gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc tgaaggttac
     2821 aatcataaaa attttatgga acaactgaag gcgattgaag aagatccaac agtcaaaggt
     2881 atctttttag aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga aatcgctaag
     2941 aaattggata cgattcgtaa agaacatgat atcccaatgt atgtcagcat gaaaaacatg
     3001 gcagctagcg gtggctacta tatttctgct caagcagata aaatctttgc aacagaagaa
     3061 acagtgaccg gctcaattgg tgtgatcatg tcaggattga attattcagg acttctagaa
     3121 aaactgggtg tggaagatac aacagttaaa agcggagcgt tgaaagatat gggttcagga
     3181 acgcgaccag agacaaaaga agaagaagcg gtgcttcaag catatatcga cagcgcatat
     3241 agtcgttttg tagcaatcgt cagcgaggga cggaataaat ctgaagaaga agtcaaaaaa
     3301 attgctgatg gaaggattta tgatggtgtc caagcaaaag aagtaggttt agtcgatgaa
     3361 ttgggatttc ctgaagatgc tttaaaagct atacgaaagg aacaacagct tgaagatgcg
     3421 gaactagtag aatattcttc cacgccatct gggtttgcta gtacatggtt aggaagtaaa
     3481 ctagcagaat tccagggatt gaagccttct gagactagtc agatcttatc cgttcttgag
     3541 aatctaggga cagcggaatc accaaaagca atgtattatt acggaggtga gtaagcatgt
     3601 cagagctttc aaatgaaaat aaggaacaag aaaaagataa gcaacagcag cctgctgtag
     3661 gacctgactc caacttttcc caaaaggtac tgagctcttt tgaaagagag cctttgacgc
     3721 ccgaagaaat caaaaagaaa caacgtaaat ggaaaaagta tacggacaca agaaatgaac
     3781 cgccaaaagc aaatgatttt cctaagtatt tctatgcagg tttttttatc cgaatgtttg
     3841 ccttcttggt cgatcttttg tgtatcggag ctattacgcg tttaacactt ggtattgcca
     3901 aaaatctagg ttggcttaat ttttcagatt cttatctgag tctctacgga ttcacagcct
     3961 tacttattta tctagcgtat tttattttat tgacgaaatt caatcatggc caaacgattg
     4021 ggaaaatgat ctttggtatt cgagtcatca gctttgatga agaagaactt agctggtcaa
     4081 ccgttttagt tagagaaggt gcttgccggt tcattctaaa atttcctttg ctgatgttag
     4141 ggtatcttcc agcagtcttt aatcagaaaa agcaacatct gggcgatttt ttcactaata
     4201 caagtgttgt tacgatcaat ctgatcaaag cgttcaacca ggaagtcaac agctgatgag
     4261 caataaaaag cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa atctagcttt
     4321 tagagggtgc ttcagttatt tttaattgtc atgctttttt tgttttgcta ctattgaaca
     4381 tgagctgaat atgagcaaga ggaggacact gcgtgaagcc gattgatttt ccagaaaaat
     4441 atgagaattt aatgcgagtg gcacaacaag cactagcaaa ccagcaatat cagcaggcaa
     4501 aagaattatt tcaacgagct tacgagttga aagaaagctt tgaggctaat agcctgcttg
     4561 ttttttgtct ttacgagttg gacgagaaaa aagaagcatt gaagcaagca ctcttgcatg
     4621 aaaaacagta tcttcaaaat gaggagtttg ctgaatttta ttttgacttg ttaatcagta
     4681 cacaagattt tttatatgct agaaaattga tagcaagtac agatttttat gaagctttcg
     4741 agcagcgggt cattgaaaaa atccaatttg cagaagaatt atccggacaa atggagaggc
     4801 agaagatcaa ggcactccac aataaatcgg aggaacttcc ttcgttggaa cctgcacgtc
     4861 agcttgcgtc tattgatgag atcgaacagc ttccttatca tgagtttatc caaacagcga
     4921 gtaaacttat tgtatcgcca gaggtccata ttttagcaag ggcaaaatta ctggagacac
     4981 ttaggcaatt gaacgaatgc catcctgttt tctatttgac gattgaagaa aagttagtca
     5041 aagtcatacc aaaagatctg ccaaagccgc agcagcaggc aagttaccag caattatgtg
     5101 tattttcaga tcgttacggg aatgaagatg cgctgcttag ttccgtgtta aaagaagaat
     5161 tcacgttgca atctgcaatt gtttatcctg tctatgatac atacatcgaa gacccgaagc
     5221 gctggtttca actgactgta gaggcgtgta caggaaaaac gatgtatgaa ggtacagaag
     5281 atgaaaaaca agatttttta aaaaaaagag aaaaaatttt gcagcaaatg atcttcttcc
     5341 attagatttt atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt ctatccattc
     5401 aatatgttta caatttgaaa gggttagtgt actatttaaa ggtatgcatg aaacgttttt
     5461 gatgtataaa atgaatttct aaactcggag ggaattaaat gactgcaaca tttgaaaaaa
     5521 aaggcacgaa tgatggtgtt ttaaaattta cgattgcgca agacgaaatc caaaaaggat
     5581 taacaactgc gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc cgtaaaggga
     5641 aagtttctcg tcaagtattc aaccgtatgt acggtgaaga agctttatat gaagatgcat
     5701 tgaatgctgt attaccagaa gcttatgaag ctgctgtcaa agaagctggc atcgatcctg
     5761 tttctcaacc aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg gtaatcactg
     5821 ctgaagtaac agtgaaacca gaagtaaaat taggacaata taaagatcta gttgttgaaa
     5881 aacaagaccg tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt gaacaagaag
     5941 cacaagctga attagttatc aaagaagatg ctgcagctga aaatggcgat acagtagtaa
     6001 tcgacttcga aggctttgta gatggcgaag cgtttgatgg cggaaaaggc gaaaattact
     6061 cacttgaatt aggttcaggc tcattcattc caggttttga agatcaatta gttggtcata
     6121 aatcaggtga aagtctagat gtaaatgtaa ctttcccaga agattatcaa gcagaagatc
     6181 ttgctggaaa agaagcagtc ttcaaagtga caatccatga agtaaaagca aaagaagtgc
     6241 ctgaattaga tgacgaattc gcaaaagatg ttgatgacga agtatcaaca ttagacgaat
     6301 tgaaagaaaa atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg gacgatgcaa
     6361 aagatgaagc agcgatccgt atggctgttg aaaacgcaga aatcgttgaa ttgccacacg
     6421 tgatggttca tgatgaagta catcgttcaa tggacgaatt cttgaacaac atgcaacgtc
     6481 aaggtatctc tcctgaaatg tactatcaat taactggttc tacagaagaa gacttgcata
     6541 aacaattcga aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc gaagcaattg
     6601 cagctgctga aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc caagaattat
     6661 cagaaacata caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa gatatgctgg
     6721 aacatgatgt gacaatgaaa aaagcaatcg aattgatcac aggtacagcg acagaagcga
     6781 aagaatagaa atcaagtaat aaatcatttc tgaagaagct acttctagaa aattgtttat
     6841 tcgaaataag ggactgtgac acgattttgt cacagttcct tattttttgt taagagtaaa
     6901 aaatgcaaag gtataggtca gttgatcctt tgttaaacac tttttctttt tactaaacta
     6961 tgttatgata gtaaaagact gtaaagtctt tgatagtttc ttgcatactt tttgcaagtt
     7021 aaggggtgaa gaccatgtat gacaatccaa gtagcaatga gaccgtacgc tgttcttttt
     7081 gcgggaaaac ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc tacatctgta
     7141 atgaatgtat cgacctatgt aaagaaatca ttgatgaaga attttatgaa gaagctgtac
     7201 gcgaattcac tgaagtccct aaaccactag aaattcttga agtatttaat aactacgtga
     7261 ttggacaaga tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac tataaacgtg
     7321 tcaatcagca agcacaagaa aacacagatg atgttgaact gcaaaaaagc aatatctgct
     7381 tgatcggacc tacagggtct ggtaaaacat ttttagcaca aacattagca aaaacattga
     7441 atgtaccttt tgcaatcgca gatgcgacaa gtttgacaga agctggttat gtgggggaag
     7501 atgtagaaaa cattttgctt aaattgcttc aatctgctga ttacaatgtg gaacttgcag
     7561 aaaaagggat catctatatt gatgagatcg ataaaattgc gcgtaaaagc gaaaatgtat
     7621 ctattactcg tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa attctagaag
     7681 gaactgttgc aagtgtgcca cctcagggcg gacgtaaaca tccacatcaa gaatttattc
     7741 agattgatac gacgaacata ttgttcatcg taggtggtgc atttgatggc atcgaaacga
     7801 ttgtgaagaa tcgtatgggc gaaaagacga ttggatttgg tacacaaaac caaaaacttt
     7861 ctgaagacga aagtgtcatg cagcatatca ttcctgaaga tttactaaaa ttcggattga
     7921 tccctgagtt tatcggacga ttacctgtta tggcagcttt agagaaattg acaacagatg
     7981 atctggtacg gatcttgact gagccgaaaa atgcgctagt caaacaatat caaaaacttc
     8041 tttctctaga taataccgaa cttgaattcg aacctgaagc gttgagagcc attgcgaaaa
     8101 aagcaatcga acgcaatacc ggtgctcgag gacttcgttc catcattgaa gaaatcatga
     8161 tggatgtgat gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt atcacaaaag
     8221 aagcagctga attgtctggt gagccaacag taatctataa agattcagag aaaaaagcgg
     8281 gttgatgaaa agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc gcttgcttta
     8341 ttcatcacct tgttaaggag agagaccatg aaagtccatc aagctgaaat tatcataagt
     8401 gcagtggcac caaaacaata tccagataca caattgccag aaatagctct ggcaggacga
     8461 tcaaatgtag ggaaatcttc ttttatcaac acattgattg atcgaaagaa tttagcccgt
     8521 acttccggta agccaggaaa gacccagaca ttgaattttt atttgatcga gaacgcatta
     8581 cattttgtcg atgttccggg atatgggtat gcaaaagtat ccaaaactga gcgagcaaaa
     8641 tggggaaaga tgatcgagac atatttaacg actagagacc agctacgtgc ggtcgtttct
     8701 ttagtagatt tgcgtcataa gccttctcaa gaagatatcc agatgtatca atttttgaag
     8761 tattatgata tccctgtaat tgttgttgcc actaaggcag ataagatccc tcgtggtaaa
     8821 tggaacaagc atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa tgacacgttt
     8881 attctctttt cttccgttac aaaacaggga aaagaagaag cgtgggcagc aatcgaacaa
     8941 gcaattgaat cataaaaaga ataaaaaaga cttaccagtc aaactctttc ataaaagttt
     9001 ggctggtaag tctttttttg agcttaactt acttttgttt cttgtctttt ttcttttcat
     9061 tttgtggctg tggttttaga aatcgatcga tctcttcttt tggtgcaact gttttttccg
     9121 gatcgacagc aaatgattcg aaaagtttat ctaaattatc tgtacgttca aatttcaacc
     9181 ccattgtaaa cacctctttg tttcttttta cttagtctat catgta
//
LOCUS       DACDID010000104         9187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDID010000104 DACDID010000000
VERSION     DACDID010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.105"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            77..2845
                     /locus_tag="IV236_001996"
     CDS             77..2845
                     /locus_tag="IV236_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772178.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTENVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            2926..3345
                     /locus_tag="IV236_001997"
     CDS             2926..3345
                     /locus_tag="IV236_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8772179.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3357..3827
                     /locus_tag="IV236_001998"
     CDS             3357..3827
                     /locus_tag="IV236_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8772180.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3844..4623
                     /locus_tag="IV236_001999"
     CDS             3844..4623
                     /locus_tag="IV236_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772181.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4610..5431
                     /locus_tag="IV236_002000"
     CDS             4610..5431
                     /locus_tag="IV236_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8772182.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5448..6497
                     /locus_tag="IV236_002001"
     CDS             5448..6497
                     /locus_tag="IV236_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8772183.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6510..7514
                     /locus_tag="IV236_002002"
     CDS             6510..7514
                     /locus_tag="IV236_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8772184.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8147..9166
                     /locus_tag="IV236_002003"
     CDS             8147..9166
                     /locus_tag="IV236_002003"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8772185.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3060 a         1538 c         1940 g         2649 t
ORIGIN      
        1 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
       61 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
      121 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
      181 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
      241 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
      301 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
      361 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
      421 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
      481 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
      541 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
      601 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
      661 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
      721 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
      781 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
      841 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
      901 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
      961 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
     1021 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
     1081 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
     1141 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
     1201 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
     1261 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
     1321 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
     1381 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
     1441 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
     1501 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
     1561 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
     1621 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
     1681 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
     1741 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
     1801 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
     1861 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
     1921 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
     1981 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
     2041 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
     2101 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
     2161 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
     2221 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
     2281 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
     2341 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
     2401 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
     2461 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
     2521 agagaatgtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
     2581 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
     2641 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
     2701 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
     2761 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
     2821 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
     2881 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
     2941 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
     3001 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
     3061 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
     3121 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
     3181 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
     3241 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
     3301 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
     3361 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
     3421 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
     3481 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
     3541 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
     3601 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
     3661 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
     3721 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
     3781 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
     3841 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
     3901 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
     3961 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
     4021 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
     4081 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
     4141 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
     4201 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
     4261 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
     4321 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
     4381 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
     4441 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
     4501 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
     4561 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
     4621 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
     4681 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
     4741 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
     4801 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
     4861 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
     4921 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
     4981 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
     5041 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
     5101 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
     5161 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
     5221 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
     5281 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
     5341 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
     5401 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
     5461 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
     5521 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
     5581 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
     5641 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
     5701 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
     5761 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
     5821 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
     5881 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
     5941 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
     6001 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
     6061 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
     6121 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
     6181 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
     6241 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
     6301 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
     6361 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
     6421 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
     6481 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
     6541 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
     6601 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
     6661 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
     6721 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
     6781 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
     6841 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
     6901 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
     6961 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
     7021 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
     7081 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
     7141 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
     7201 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
     7261 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
     7321 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
     7381 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
     7441 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
     7501 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
     7561 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
     7621 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
     7681 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
     7741 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
     7801 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
     7861 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
     7921 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
     7981 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
     8041 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
     8101 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
     8161 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
     8221 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
     8281 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
     8341 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
     8401 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
     8461 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
     8521 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
     8581 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
     8641 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
     8701 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
     8761 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
     8821 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
     8881 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
     8941 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
     9001 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
     9061 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
     9121 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
     9181 gttaatc
//
LOCUS       DACDID010000105         9041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDID010000105 DACDID010000000
VERSION     DACDID010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.106"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(67..2241)
                     /locus_tag="IV236_002004"
     CDS             complement(67..2241)
                     /locus_tag="IV236_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8772186.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2257..4599)
                     /locus_tag="IV236_002005"
     CDS             complement(2257..4599)
                     /locus_tag="IV236_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772187.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4621..7164)
                     /locus_tag="IV236_002006"
     CDS             complement(4621..7164)
                     /locus_tag="IV236_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8772188.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7174..7572)
                     /locus_tag="IV236_002007"
     CDS             complement(7174..7572)
                     /locus_tag="IV236_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8772189.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7653..7937)
                     /locus_tag="IV236_002008"
     CDS             complement(7653..7937)
                     /locus_tag="IV236_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772190.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(7961..8959)
                     /locus_tag="IV236_002009"
     CDS             complement(7961..8959)
                     /locus_tag="IV236_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8772191.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2575 a         1955 c         1421 g         3090 t
ORIGIN      
        1 gtttttatca gtttaaaaca accaaataag aggaaactaa caataagtat cctaacaata
       61 actttcttat ttcgcatatt gtttctccaa atttggtttc cgaatataga cgactcgcgg
      121 tggttcctgg acgtcttcag ctgttcgata caccacaaat tcgcctgttc tcaatgctcg
      181 aatgcgattc ggattgaatt taaagcggtc tacgtctcgt ttcgtgcctc tttctcctgt
      241 ccaatcaacc tttcccatat cagaataacc tgcttcttgt gtcgtcattt ccgttaattc
      301 aatgtcttct tctgtcccca ttaaatctgc ccaataatca atttctgtcg ggtcatttgt
      361 cttcccaata aagaacgtat tcacattccc aatcaactgt tttcgaagga caggatcaat
      421 tctatcaatg tccgcaagcg tttgaggggc atacgccgca tgcaccccag cgcctcgtcc
      481 cttattgact aagtctataa ggttatcatt tagataagaa gctggttcgt caaatattac
      541 taggactttc gagcgattgg ctgtttctcc tgcttgttta tacacttctg acgcaaagaa
      601 attaatatct cccacaagca tttgagccaa tgtccgaata aactcactat aaatcaatcc
      661 gtttaacgag atataaacaa tctttccttc tttaacaact tcttgaatat ctaattctct
      721 ttttcctcca gaagtatcaa agagttcacc aagctcacta tccaataaca tattgatatt
      781 ggtctgtaaa ttctcagaag tcgcatacaa ataaaacggg ttcttcttat gttcgtaacg
      841 aacaaacaac cgtttaaaca acgtttgctc gatttctgtc atgagagaaa tgttttcttt
      901 taataataga taatacgttt ctaagtcaac ggttttacga tcatgttcaa cgattcgctc
      961 ttcacttaac tgttccacat cggcaaaatc acgttctgtt ttgtccatcg cttcattctt
     1021 tttcgttttt ttcttcgtgg ttttcttctt ttctacgacc actttttctt taaataaatc
     1081 tggatttttc aactgaatac gatccgcaaa caaatgtaag acgtttcgtg gcaataaata
     1141 atgttgtaac caaactagat ttctttcaat tttatggctc ttttggaact cgtctaaaag
     1201 ctgaatcgtt acctgtaata acgctttggc tgctgtcgaa taatacacgc tttctgtttc
     1261 tgctagattg gttaatttat ctcgaatggc aatatcattg ccatttttta caggattata
     1321 acgcaaatcg ccaccgtcca caaagacttc aatcgggcga ttgtatgcct ctgccacttc
     1381 ttttaacgct tgtaaagtct ctcctgctcc tttcccatcg actaagataa cggggatacg
     1441 aaacttacac gcatgttctg ctagtaattg gatcaccgtc gtttttcctg aacctgtagt
     1501 cgctggaata atcacatgtt ggttcaattc actaaagttc atcacataag ggagcccata
     1561 ctcatctgtt cctaatagaa tcactttggc taatgaagcc agttgtttat tccgcaaagc
     1621 aacgtctttt atgacaattg ctacctggta cgcttgctga tactctgctg tatatttttg
     1681 ggactcctga aatttattct tccgacgttc cttgtattct ttactattta gcaaagctgt
     1741 ttgccgttct ttctttgaaa caacttttct tttttcaata aaagacgcat acgcaatcaa
     1801 acaacttcct gcacctgtcc cacctaaaag gagggcaaaa atcgtttgtc cattaaactg
     1861 caacgcatga gaaaacaagt ttaatggaaa taataagtaa cacgacaaat accctctagg
     1921 aagaacaaaa ataaaacaaa atagatttaa tagcgtaaaa cctaacgcaa aaataaaaaa
     1981 aagagagctt cttcgtttta agaagctctc atactcaaag tcataaatat gtgcacgtgt
     2041 ctctttttta tctatcataa agacaatcgc taaaggaata ctgcctacaa taaaaaatgg
     2101 atatagacaa acacctaata aagtgacgat aaaataaagt tgttgaaacc gactttttgg
     2161 cgtttctttt tcttgttttc ttttgcgatc gacagaaaac atttgctcaa aatgtgtcgg
     2221 tcgttttggt gcattcatca tactatcgat ttcctcctat cgttttcgtt gtttcatttc
     2281 tcttcgataa atcggttgtt tttctcgttt tcgtttcatt tttgcttgag ttgctcgaaa
     2341 ctcactccgt gtcttcggtg cttgttttgg tgattttgtt tgtttcatgg ttgagtatgc
     2401 tttagatacg gttgaaggcg ctttggtttg tgctgttact cgattgactg ctgacatatg
     2461 ttcccgaacc ccctctggac gtcgaacaga tttcggtgtg gtagaacctg ttgctcgtgg
     2521 tacattccct tttcttagtg gtttttgtac cgcatgtttt tgtcctgcaa ctgtccgggt
     2581 tttcatcgaa cttgtttttg ttacgggagt aagtgcctcc ttccgacgac gacttgtttg
     2641 tccctgacga cgttttcgat cgttcaattc tgttctaggc gtatgcttct ggccataaga
     2701 cttgtgtttt tgctttggat tcgctaatga caccaccatt gcagaaccct gattgcgata
     2761 cggactacga gacgcacctt ttctacttct ttctcttaga ctaggattgc cttgcaattc
     2821 tcgttgtaat aaatcggctg tttttcttgc ctgtttctcc attgctgttc gttgcatacg
     2881 ttcttgactt tgctcttctg cttgtttcgc ttgatcatgc aagtctcttg ctttttgtgc
     2941 catacgttcc atacgatttg gtgctttatg acgaggttgt tgtgcggatt cacgccgaaa
     3001 agcacttcgt gtggcttgtt tcgccgtttg tggcatacga acgtctgagc cacccccacg
     3061 ttttcgttga cgttcttgtc tcttccgtgc gttttctttt tctttttgtt tctgtgcttt
     3121 tctcgcttct ttgcgttctt tcaaccgttg tttggcttcc cttctcaatt ttgcttctgc
     3181 cccaaatgga tttcccgcaa aagctaacgc tcctcgaaca cccattcttc cagtaaatag
     3241 actgcctaaa aagcctcgga acaaataaat tcccaacggc gcaaacaaat aaacaatgat
     3301 caagagaatt ttttcccatg gatcgtcttg tgtactactt aattggaacc cgtaactaag
     3361 aaaagaagaa aaaaggatac atgcaaaacc tgtcattcct tttaaaaacg tcaaaatacc
     3421 aaaaactttg gcatagttac caaacacatt cattgtgaca agaaacaatc ctaaaaaaag
     3481 taagaacggc aacatcggta ctaacaatag ttgcatagtt ccaataatca atcgaaacat
     3541 acttaagaca aaataaacaa cggtttgaat cagattcacg acaaaataaa acaaggcaaa
     3601 tgctgtattt ttccaactat tgtaatattg tatgttcttg tttttcagtg tatctgcttc
     3661 atactctgtg actttgtcat gaattttctc ccctattttt gtacctaaat cattgtccaa
     3721 taaaagattg attcgatcat attcttttcc agcatatttg actttcttct cacgaattgt
     3781 gtctacattg gttgtgccat aatttaacaa cagatatggt tcataaacat tactataaaa
     3841 gacccgagcc gccatcaatt tgcctgccgt ttccactttt ttatccgctg ttacctcttc
     3901 tgtttctttt gtttcataat ttgtttcttc ttcaggtgtt aacactggat tgaccttcac
     3961 gaacgccgtt tccactgtag aatctaaaga aaaaagaaca tcaaaaagat tcttatttgt
     4021 cccagaatct tttaatacgc ttaacccagt aaaaataagg atcgtcataa gaaagacacg
     4081 gagtgcttgt ttcattcgag cctctccagc atatttgatt gccatcataa ttgccgaaaa
     4141 actgattcca atagttcctg caatacttaa catattgtcc gcaatttttt gtgttaagtt
     4201 ttgaatgggt tctcgaatgg cggaaataat atctaattca aagagaaatt caaccattga
     4261 agcattgaat tctcctaaca tttttacgcc actccaaaca aaatttttca tcactcctgc
     4321 aacattgtac gatagctttt ttttaaggtt cattgactca tagtcattgg tcatcaattc
     4381 aaacgaattc tttttgtaat ttttatacaa ttgatccaca tcagtcgtta cttcttttac
     4441 ttctacttta tcgttggcat tctctccttc agaacgatcg gtttcagtga cacccgcacc
     4501 atcattcatt tgatcggcag aaacagtagc cccttgtaga cagaaaaaaa gaccccctaa
     4561 taaaaggagc gtccaccatt tcagagattt ttttcgcaaa aaaaccacct cttttttgtt
     4621 ttatcgttgt ttttcatttt ctaacattgc ttgaattttt tcatcttgat tttcagtaga
     4681 agaaacagcc aataaccaac tatcaaacaa cacatcaatg gcaatcggtt gatttcttcc
     4741 caaatgatcc tggaataaac aggttcctgt ttgtaattgt tccatcattt ctaaattggc
     4801 tgaattcact tccatatcaa agaaggacaa tagtttctct cgtgcttctt tttgtttcgg
     4861 acgaaacgca aatttgtaag aaattaactc ttttttatct tcttgatcgt aatctttaaa
     4921 cgcttgcaca acaaggtaaa tatccgtatt attcgcacgt ccttttcgca agctatcatc
     4981 aatcaaatag cttccttgtg cggtatcttc ataccctttt gcctcgtcat aaataatcgc
     5041 agcgtcttct tctttgtttg tagaaaagat atacgtaaac ttcataatga cttccataat
     5101 tgccattcct gcaagttgtt cgctatttaa tcgacccgaa tcaatctctt tttgtgtcgg
     5161 aatgcgtatt ccttgtgtcc ctaacacgtt gatttgtgaa tcaaaacgta tcggcgtact
     5221 gtgctcattc ccaatgagaa tctttcctaa accaacgcta tgccctaaaa tcaatttagc
     5281 tagttctttg tcactttttc gaatttcttc gataacttga cttaacgaat gcttacctgg
     5341 catggtgtaa actacatcga tggcgtcttg gatcaccgtt ttcttgtggc tcgctgtttc
     5401 tcgattggta tctacttctc ctaacattcc taacacattt ttagcggttt gaattgcctc
     5461 ttcttttggt aaaaacaata gaggatcaag catccctcga cagctttcat ctgaagataa
     5521 cgaaataaaa ttaattcgtt catacatagc cacaaactct ggtatgtgtc cgaatttttc
     5581 aagtgccttt aaaaagaact tttgggtttc atttttcgga tcaatataaa gaattttttg
     5641 acctaaaaac atcagccata aaaaaatata ttttacaagg accgattttc ctttacctgg
     5701 tggaccaata atcaacgtat ttccattggt atgttccgcg ccttcaatac ttttctttgt
     5761 taaattggga taataccata ctagtttact agatagcgct aacgcttctt tcgcagattt
     5821 aaactttttt gcaataacaa tccgtcctaa aggaaatcct ttcttatttc ctacttcttt
     5881 ctttaagtcc aagccataat ctgccacaaa acctgtcgta gcgacctgct cgtaactacg
     5941 aaaacgttgc gtacttccaa ttaagcattg attaaacaac gttagctgat ccgctaccga
     6001 tcgataaaat ttgaaatctg tagactcaat cgcaaattca atatctttga ctcgttcttc
     6061 caattcttct tttgaagaag cagatacaac cagcgtgacg gtagaataac acaatcgttt
     6121 ttctttgcgc ttcagcttat cttttacatc cattaaccgt tctgtaccaa aatttaatac
     6181 atcgtctgaa tctaaaatgc cgtcaacggt ctcatattct ttttcttgtt cataaatccg
     6241 tccagccatt ttattaattt ttcgttcatc agattttgaa tgctcaaatc gaagtcgaat
     6301 atgcgtatca attggaaaag ataagctatc ctgaagattt tgaataaagc tactcccata
     6361 catggctaaa ggagcgtccg tttgacttaa aaacgtcgaa taatgcgttt gattcaactg
     6421 ttcaatcgca atgtatcctg cttcattcga aatgacgccc tcgcaaattt ccattggcgt
     6481 aagctgtctt tcaggcaatt tttcttctga tcgatggaaa aaataataat aaatttgtcc
     6541 aatctcttgg gcagataagc gctctatcgg cttataattg gacaattccg tatagagctc
     6601 cttttctaat gtttcatacg ttggtaacac actacttata ggaactcgtt gtcccgtaat
     6661 acgatagata aactttttaa aaacatgctt aagcaattga aaatattcga tcggatcaga
     6721 cactttcatt tctgtagtga aacgcacaaa cagatacgtt cgatactcgt ttaattgcac
     6781 ttcgtctttt aaaatctttc ctgcctgttc aaagtacact ttcccaagat ctgcgcattc
     6841 tccctttacc aaatctcgaa tggtttcttc aatgtgcgta tctaggttaa attgacgtgg
     6901 aatattgata aaatgatagt catattcatc atgtccaagt atgccttcgc catcttgaag
     6961 atagttcttg aaaaaatcaa tgtcatttaa aggaaatact tgggaagaaa gttgatagcc
     7021 tacccacaca tcatcatttt tggtcaaaac aaaatttcgg ataatggcgt ctatcggaaa
     7081 ttctaatcga ggaagcttcg tttttttctt tttgaactgc caaatcccga taataaagac
     7141 gaccaaaaga aacaaaaacg ccatttactc gactcactcc ttccatttgt gtagggaata
     7201 cgtcactttt tcatggaaaa acggcacata catttcatgt tcaaagcgtt gtttccgttt
     7261 ctgataatag gacgttcgcc caattaaata ctcgaaaaag tgtttattgt cccatttcat
     7321 tgaaaacaat gtccaaacga gaacaccaat cccaaaaatc ataatcaacc aggactttcg
     7381 aaacagtgtc tgcaagaaac taatttgatt cacatacgca atcaaaaata ggatcaaaag
     7441 aatgaagaga aataccccac ctatgactaa ttgttccatg cgaatgccat tagaaatcca
     7501 gggaattttt tgtcctttaa tcccgtaaat tttgtgaggg tgcttaaact cctttgtata
     7561 gttgtattcc atgttcactc tacctccttt tacaaaaaaa gaagcgacca gaagttctaa
     7621 tcttctgatc gcttttgcag tttgtctttt tcttatgata attgtatcca gggcacaatc
     7681 atttgcgtta cccccacgtt ctcttcacta tccttaataa agataatgtt ctttagccat
     7741 tcaccaatat caacggcatt cgataacaat aagacgagca ttatcaaaat aaagacgcca
     7801 ataatggcaa acgctgcccg cgcaaagcct tgggtcgcat aagcaccaac cattagaaca
     7861 atcgcaacga ttgccaatag ccaaatgact tgtccagata catcatcaaa taacgattta
     7921 agatcaaaaa gtttcatttc cattctcctc taagttcatt ttattcattt aacgtagtaa
     7981 caaaataact atcgccactt gtttttactt ccattgaaaa gtttgattgg aactcgtttc
     8041 ctttctcaaa atagaaacga tacttccctt gcaaaaagta gtttccttct ttatttttta
     8101 ctgcatgatc cagttgtaca ttgatcaatt cgccgccttc taaccctttt tcgttagaaa
     8161 tcaatgccaa tttttcatca tttttgacat ataactcaaa aaaccgtttc acaaatgctt
     8221 ccagcttgat ttcttcgctt tcagtgaccg cttgcccaga agaataaaat cgattttcct
     8281 gatagttcac agaattggta ttcttactct cagaaagatt caaaaattga acgccatcaa
     8341 gtacttttaa aacattgttt tgataactga ctggaaaaga aagttgactg cttttccaat
     8401 gttctccttc tgtaaaagta acctcataaa ataggcgata cattgcttgt gtcaaatcga
     8461 tttcttctag tttaataaag cgaacatctt tcgctactcg attggtagcc gtcgttacaa
     8521 agcctagatc agacgcttcc aatccttgtg ctaaataatg attgacggaa gcctcccatt
     8581 cgtttcgttt ttctggcgaa taagtaaata acctttcaat aaaatgttgt ccctcgtact
     8641 tcaattgttc cgtagaagac aaggtttggt tgacttcagt taacaatgtt tctggattga
     8701 cttgtttttt cactgctaaa cgcgttaatg tatccactcg tccaaagttt aggaggagca
     8761 cgacacccat taacagaaac aaacaaaaaa aagcgcctga aaatacactc atggcacttt
     8821 tttgcgaaaa ttggatcatt cgatctgacg gttctgtttt ctttttttgg aacaaaaaac
     8881 gtttcttttg tttcttttcc ttctgttgtt tcggtggctt agtttggaac gcttgtggat
     8941 taaattttgc attactcatg agaaatgcca taccgttcta acaataattt cggtattttg
     9001 tccacaatca ctacttcgtg gacaaccaca cttgccccct c
//
LOCUS       DACDID010000106         8909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDID010000106 DACDID010000000
VERSION     DACDID010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.107"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..766
                     /gene="ubiA"
                     /locus_tag="IV236_002010"
     CDS             <1..766
                     /gene="ubiA"
                     /locus_tag="IV236_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HAP8772192.1"
                     /translation="YEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVIL
                     TINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFG
                     SDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHR
                     YTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFK
                     QTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1701..3047)
                     /gene="gor"
                     /locus_tag="IV236_002011"
     CDS             complement(1701..3047)
                     /gene="gor"
                     /locus_tag="IV236_002011"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8772193.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3181..4422)
                     /locus_tag="IV236_002012"
     CDS             complement(3181..4422)
                     /locus_tag="IV236_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8772194.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4440..5363)
                     /locus_tag="IV236_002013"
     CDS             complement(4440..5363)
                     /locus_tag="IV236_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8772195.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5584..8076)
                     /locus_tag="IV236_002014"
     CDS             complement(5584..8076)
                     /locus_tag="IV236_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8772196.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8083..8550)
                     /locus_tag="IV236_002015"
     CDS             complement(8083..8550)
                     /locus_tag="IV236_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8772197.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8686..8759)
                     /locus_tag="IV236_002016"
     tRNA            complement(8686..8759)
                     /locus_tag="IV236_002016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8723..8725),aa:Arg,seq:acg)
     gene            complement(8817..8890)
                     /locus_tag="IV236_002017"
     tRNA            complement(8817..8890)
                     /locus_tag="IV236_002017"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8854..8856),aa:Pro,seq:tgg)
BASE COUNT         2474 a         1883 c         1483 g         3069 t
ORIGIN      
        1 ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg gccgcagaaa
       61 tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg tttttgttat
      121 cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat ttatctgttg
      181 ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc ctttagggga
      241 aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt atgtcaatac
      301 attcggttca gatgtctatg atctgacagt caacttttca tctggacaat ttgatttggt
      361 aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg tgtttacgat
      421 tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg aaaatcatcg
      481 ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc agctattgat
      541 gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg aatggccgat
      601 cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat tcaagcaaac
      661 gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat tcaatgccag
      721 ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc atgaagaggt
      781 tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata gctccttata
      841 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttatcagga
      901 ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
      961 acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcag
     1021 aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     1081 cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatca
     1141 gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa aaatagttcc
     1201 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ctatttgttc
     1261 ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca cttgatttga
     1321 ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg tattctttta
     1381 acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt aaacatctgt
     1441 tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac ttaagagtgc
     1501 ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat ctccaacttc
     1561 cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact aaaaaacaaa
     1621 aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa taaaaagctg
     1681 ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc tgttggatgg
     1741 atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc cacggcaaaa
     1801 ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat tctttcttct
     1861 tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa tgcaaagtac
     1921 attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt ttccaatgct
     1981 tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac tgtcggaaca
     2041 agtttatagt caagataaga atctttcttt ccattaaata aacgttctga taaacgtcta
     2101 ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc aacagcaaaa
     2161 atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt tttgtccaat
     2221 tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc accggcaaaa
     2281 agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata ttcgtcattg
     2341 tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc ttcttggtac
     2401 atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg ttcttttcga
     2461 tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc agccgcaatg
     2521 tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa aaatccattt
     2581 gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc tccagtagcg
     2641 atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg actatcgata
     2701 aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct ttgataagca
     2761 ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa atcaacattt
     2821 tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc caaaatcgta
     2881 cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt tccgcccaat
     2941 tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt tgctgaagca
     3001 ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt gttcctcctt
     3061 attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa aacaagtgta
     3121 acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc agctttttcc
     3181 ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg gcatagttaa
     3241 aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat gcataacttc
     3301 aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat ctcctacaga
     3361 aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag ataatacttc
     3421 accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt cgttttcagt
     3481 tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc ataactcgtc
     3541 gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt ctacagccgc
     3601 tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc cttcgatctt
     3661 gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg acatatccac
     3721 tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt ttcgttcttg
     3781 accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac atccgccacg
     3841 gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat aagagataca
     3901 catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa tctctgccac
     3961 ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt ctttccagaa
     4021 ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg gcagacctgg
     4081 ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg cagaaatacc
     4141 gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt catgggtgac
     4201 catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa ctccttcagc
     4261 catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat tcccgccaat
     4321 atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg ttcctgctgg
     4381 cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca tcatactcct
     4441 tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg gttttcagga
     4501 tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca gctaccttct
     4561 aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt ttcgcctgga
     4621 gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc attcaattca
     4681 ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc ttcataaata
     4741 gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc acgatctttt
     4801 tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt ggattgatgg
     4861 atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc ttccatcgcc
     4921 ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc ttttttcgcc
     4981 cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat atacggtata
     5041 cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac tgtgatcaca
     5101 tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat tttttcagga
     5161 tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc taattctacc
     5221 aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa ttgctcttca
     5281 ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc aatagtttct
     5341 actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat agtagcatag
     5401 ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa ttatttcaca
     5461 aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt gtgacaaaaa
     5521 tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac acaggccaag
     5581 taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg tgatcttacc
     5641 ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc
     5701 gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg caccatattc
     5761 aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt tcacttgaat
     5821 atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga ttgtatggat
     5881 ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca agaattcagg
     5941 tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt gatctttagt
     6001 aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg ctccgatatt
     6061 tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag aatctgttaa
     6121 atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg ctttctccac
     6181 ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca actgtccacc
     6241 ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac tgtatttttc
     6301 cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca tgacttctga
     6361 aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca tgaatgaacc
     6421 aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc
     6481 tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca gttctaacaa
     6541 gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact ccgatacaac
     6601 tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt ttgcttcaat
     6661 tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta attgagcagc
     6721 attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt catcgatttc
     6781 tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga ctttcgctgc
     6841 tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga tataacgaac
     6901 agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat ggtgctcttc
     6961 atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg gctcatctac
     7021 ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt tttgatattc
     7081 atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat
     7141 attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat gcaattcatc
     7201 aatgaataag attacttgac catcattata gatttcgtcg atgacctttt tcattcgatc
     7261 ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa gcatcatcag
     7321 gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag ctaacccttc
     7381 agcaatggct gttttgccta caccaggttc acccaccagt acagggttat tctttgtccg
     7441 tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag
     7501 cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc
     7561 ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca ttccttttcc
     7621 agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt ttgaaaggct
     7681 gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta ataagctcat
     7741 caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc tgctagctaa
     7801 agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag ggtaagaacg
     7861 gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt cattcaaatc
     7921 catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg ctagtaaaac
     7981 atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg caatttctaa
     8041 tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc catcttcata
     8101 acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt catattcatt
     8161 gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt tttttgtaat
     8221 tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac tgattgtatc
     8281 atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc
     8341 aattcgtata taaccaccac caccacgttt gctttctact agataaccac gttggatggt
     8401 aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga ggtctgccat
     8461 ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta tatatgattc
     8521 aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact aatattgacc
     8581 ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta aataaatcct
     8641 ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc ccaagaggag
     8701 tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc tatgggcgca
     8761 tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa aaaaaatcgg
     8821 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     8881 ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDID010000107         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDID010000107 DACDID010000000
VERSION     DACDID010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.108"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IV236_002018"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IV236_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IV236_002019"
     CDS             complement(1456..1791)
                     /locus_tag="IV236_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8772198.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IV236_002020"
     CDS             complement(1775..2047)
                     /locus_tag="IV236_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8772199.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IV236_002021"
     CDS             complement(2498..3301)
                     /locus_tag="IV236_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8772200.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IV236_002022"
     CDS             complement(3279..4403)
                     /locus_tag="IV236_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772201.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IV236_002023"
     CDS             complement(4419..5234)
                     /locus_tag="IV236_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8772202.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IV236_002024"
     CDS             complement(5224..6105)
                     /locus_tag="IV236_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8772203.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IV236_002025"
     CDS             complement(6181..7470)
                     /locus_tag="IV236_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8772204.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IV236_002026"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IV236_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDID010000108         8844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDID010000108 DACDID010000000
VERSION     DACDID010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.109"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..655)
                     /locus_tag="IV236_002027"
     CDS             complement(<1..655)
                     /locus_tag="IV236_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8772205.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     "
     gene            789..1076
                     /locus_tag="IV236_002028"
     CDS             789..1076
                     /locus_tag="IV236_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772206.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1492..3030
                     /locus_tag="IV236_002029"
     CDS             1492..3030
                     /locus_tag="IV236_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8772207.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3027..3992
                     /locus_tag="IV236_002030"
     CDS             3027..3992
                     /locus_tag="IV236_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772208.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3979..5301
                     /locus_tag="IV236_002031"
     CDS             3979..5301
                     /locus_tag="IV236_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8772209.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5364..5468
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5542..6285
                     /locus_tag="IV236_002032"
     CDS             5542..6285
                     /locus_tag="IV236_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8772210.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6300..7292
                     /locus_tag="IV236_002033"
     CDS             6300..7292
                     /locus_tag="IV236_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8772211.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7304..8041
                     /locus_tag="IV236_002034"
     CDS             7304..8041
                     /locus_tag="IV236_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772212.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8031..8753
                     /locus_tag="IV236_002035"
     CDS             8031..8753
                     /locus_tag="IV236_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8772213.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2943 a         1513 c         1858 g         2530 t
ORIGIN      
        1 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
       61 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      121 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
      181 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
      241 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
      301 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
      361 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
      421 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
      481 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
      541 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
      601 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
      661 gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact
      721 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
      781 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      841 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
      901 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
      961 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
     1021 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
     1081 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
     1141 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
     1201 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
     1261 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
     1321 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
     1381 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
     1441 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
     1501 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
     1561 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
     1621 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
     1681 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1741 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1801 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1861 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     1921 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     1981 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     2041 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     2101 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     2161 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     2221 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     2281 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     2341 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     2401 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     2461 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     2521 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     2581 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     2641 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     2701 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2761 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2821 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     2881 gttaaagcac tgaataaatt aagcggaaaa ataacggagg atcaaatgat tgatatgaat
     2941 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     3001 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     3061 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     3121 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     3181 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     3241 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     3301 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     3361 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     3421 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     3481 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     3541 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     3601 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     3661 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3721 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3781 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3841 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     3901 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     3961 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     4021 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     4081 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     4141 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     4201 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     4261 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     4321 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     4381 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     4441 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     4501 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     4561 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     4621 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     4681 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4741 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4801 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4861 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     4921 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     4981 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     5041 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     5101 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     5161 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     5221 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     5281 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     5341 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     5401 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     5461 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     5521 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     5581 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     5641 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     5701 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5761 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5821 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     5881 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     5941 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     6001 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     6061 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     6121 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     6181 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     6241 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     6301 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     6361 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     6421 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     6481 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     6541 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     6601 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     6661 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6721 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6781 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6841 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     6901 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     6961 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     7021 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     7081 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     7141 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     7201 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     7261 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     7321 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     7381 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     7441 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     7501 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     7561 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     7621 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     7681 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7741 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7801 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7861 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     7921 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     7981 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     8041 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     8101 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     8161 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     8221 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     8281 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     8341 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     8401 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     8461 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     8521 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     8581 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     8641 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     8701 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8761 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8821 gattaataaa tgaatcttag tacc
//
LOCUS       DACDID010000109         8777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDID010000109 DACDID010000000
VERSION     DACDID010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.110"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..970
                     /locus_tag="IV236_002036"
     CDS             <1..970
                     /locus_tag="IV236_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8772214.1"
                     /translation="NPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHNRIYKFFYPK
                     KIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVR
                     KPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLN
                     KKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILL
                     GDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDS
                     GQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1544..2113
                     /locus_tag="IV236_002037"
     CDS             1544..2113
                     /locus_tag="IV236_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8772215.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2100..2960
                     /locus_tag="IV236_002038"
     CDS             2100..2960
                     /locus_tag="IV236_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8772216.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3150..3854
                     /locus_tag="IV236_002039"
     CDS             3150..3854
                     /locus_tag="IV236_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772217.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3859..5694
                     /gene="walK"
                     /locus_tag="IV236_002040"
     CDS             3859..5694
                     /gene="walK"
                     /locus_tag="IV236_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8772218.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5691..7007
                     /locus_tag="IV236_002041"
     CDS             5691..7007
                     /locus_tag="IV236_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772219.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7008..7877
                     /locus_tag="IV236_002042"
     CDS             7008..7877
                     /locus_tag="IV236_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772220.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7932..8741
                     /locus_tag="IV236_002043"
     CDS             7932..8741
                     /locus_tag="IV236_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8772221.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         3045 a         1440 c         1855 g         2437 t
ORIGIN      
        1 taatccaaat ttattgtttt atgactattt gcttaattat ttggaagtat caaaaaaaca
       61 agtagcattt aacacatatg ttgggtataa gcattacgtc cataatcgaa tttacaagtt
      121 tttttatcct aaaaaaatta aactaaatag gttaaagcct tatcatttac aagagtttta
      181 tcaatatatg ttaaatgata actgtacagc aaatacggtt attcattatc acgcatttat
      241 acgcaaagct cttcaagagg ctgttattac agaattgata agtacaaatg tagcagataa
      301 agtaagaaag ccaaaaaaga aacaatttgt ttctcaagtt tataatcaac aagagatctt
      361 gaaattactt gacattttgt cagaagaaaa actctatttg gtagttcttt taacagcatt
      421 ttatggattg cgaagaagtg aagttttagg attaaaatgg tctgctattg attttttaaa
      481 caaaaaaatc atgataaacc atgtaatcat agaaaatcca gagaatatgt cacagttaat
      541 aaagaaagat caaacaaaga actcttccag ctatcgtaca ttacctttgg ttgaaacgat
      601 tgaacgagca ttagtaaatc aagctaaatg gcaacaagat aatcgtattc ttttaggaga
      661 tgattatatt ttagaagata atgagtacgt atttactatg gaagatggac ggttgatgaa
      721 gcctcaatat gtaacgcatc gtttgagtaa gttgattaag aaaaatggat tgaaaaaaat
      781 tcgctttcat gacttgcgtc attctaatgc ttcaataatg ttagatagtg gccagaatat
      841 gaaatccatt caagaatggc tgggacatgc atcgtattcg acaactgcta atttatacac
      901 gcatttaacc gcaggagtaa aagatcgtgc agcagaagca ttagaaaacg catttgggtt
      961 taaagaataa aaaaacaaaa aacaatatct ttggggtatc gcttcttgtt tttagaattt
     1021 ttttgttggc aatttgttgg cagtatgttg gcaatgaaaa aattcacaaa ctaaaaacct
     1081 atttataaag ctttataaaa cttttttcac aaatagagca tttggttaag agtcagctgc
     1141 tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc actaagaatt
     1201 ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct tataggaaat
     1261 cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct
     1321 tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg
     1381 gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt
     1441 tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata
     1501 agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca
     1561 gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact
     1621 ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc
     1681 tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa
     1741 actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt
     1801 acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt
     1861 gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga
     1921 agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga
     1981 ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa
     2041 acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa
     2101 tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgttcca gaagaattga
     2161 taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata
     2221 cagataaaat tgacattacc cctgaagaga tctatcaacg tttgccagag gaaatccctg
     2281 gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg
     2341 gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg
     2401 ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata
     2461 ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag
     2521 aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta
     2581 atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg
     2641 taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca
     2701 cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag
     2761 aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag
     2821 aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact
     2881 ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg
     2941 tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc
     3001 cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt
     3061 ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta
     3121 tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga
     3181 agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgac gtttatactg
     3241 catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac
     3301 ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt
     3361 atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct
     3421 tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac
     3481 gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac
     3541 ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac
     3601 gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc
     3661 accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt
     3721 ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata
     3781 atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc
     3841 cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag
     3901 atcgctatta cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt
     3961 atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg
     4021 gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa
     4081 gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa
     4141 atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc
     4201 gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt
     4261 gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc
     4321 ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa
     4381 atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg
     4441 atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa
     4501 gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa
     4561 cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat
     4621 acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc
     4681 attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta
     4741 gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa
     4801 gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc
     4861 agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa
     4921 tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca
     4981 gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca
     5041 agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca
     5101 ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac
     5161 ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat
     5221 ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa
     5281 aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca
     5341 gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat
     5401 gggggaacga ttacttgtcg cttattagaa acgcataata acgtgatttt aagtatcacc
     5461 gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga
     5521 gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga
     5581 gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg
     5641 actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct
     5701 ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt
     5761 atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc
     5821 aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta
     5881 catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc
     5941 aattgatcat cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat
     6001 cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt
     6061 ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa
     6121 aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc
     6181 gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag
     6241 atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat
     6301 cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaagaag tatagttata
     6361 tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg
     6421 tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta
     6481 tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg
     6541 ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc
     6601 tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc
     6661 cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg
     6721 gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag
     6781 acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag
     6841 cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa
     6901 cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt
     6961 cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa
     7021 aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt
     7081 atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc
     7141 aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc
     7201 aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca
     7261 aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga
     7321 aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt
     7381 ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca
     7441 cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg
     7501 aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata
     7561 atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg
     7621 aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa
     7681 taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc
     7741 gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg
     7801 tccaaattga gagcgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa
     7861 aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa
     7921 gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa
     7981 gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac
     8041 ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag
     8101 atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc
     8161 gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga
     8221 ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg
     8281 gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc
     8341 gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt
     8401 atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg
     8461 agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag
     8521 gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca
     8581 aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa
     8641 caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg
     8701 atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa
     8761 tataaaaagc agaagat
//
LOCUS       DACDID010000110         8753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDID010000110 DACDID010000000
VERSION     DACDID010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.111"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(260..2641)
                     /locus_tag="IV236_002044"
     CDS             complement(260..2641)
                     /locus_tag="IV236_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8772222.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2631..3278)
                     /gene="recU"
                     /locus_tag="IV236_002045"
     CDS             complement(2631..3278)
                     /gene="recU"
                     /locus_tag="IV236_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8772223.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3342..3881
                     /locus_tag="IV236_002046"
     CDS             3342..3881
                     /locus_tag="IV236_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8772224.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3971..4390
                     /gene="gpsB"
                     /locus_tag="IV236_002047"
     CDS             3971..4390
                     /gene="gpsB"
                     /locus_tag="IV236_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8772225.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4412..4779
                     /gene="rnpB"
                     /locus_tag="IV236_002048"
     ncRNA           4412..4779
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV236_002048"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4988..6154
                     /locus_tag="IV236_002049"
     CDS             4988..6154
                     /locus_tag="IV236_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8772226.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6200..7696
                     /locus_tag="IV236_002050"
     CDS             6200..7696
                     /locus_tag="IV236_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8772227.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7777..8460
                     /locus_tag="IV236_002051"
     CDS             7777..8460
                     /locus_tag="IV236_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8772228.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2758 a         1583 c         1857 g         2555 t
ORIGIN      
        1 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
       61 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
      121 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
      181 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
      241 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
      301 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
      361 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
      421 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
      481 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
      541 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
      601 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
      661 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
      721 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
      781 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
      841 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
      901 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
      961 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     1021 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     1081 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     1141 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     1201 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     1261 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     1321 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     1381 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     1441 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     1501 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     1561 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     1621 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     1681 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     1741 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     1801 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     1861 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     1921 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     1981 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     2041 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     2101 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     2161 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     2221 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     2281 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     2341 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     2401 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     2461 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     2521 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     2581 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     2641 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     2701 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     2761 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     2821 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     2881 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     2941 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     3001 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     3061 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     3121 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     3181 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     3241 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     3301 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     3361 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     3421 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     3481 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     3541 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     3601 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     3661 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     3721 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     3781 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     3841 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     3901 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     3961 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     4021 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     4081 agattatgaa gcttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     4141 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     4201 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     4261 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     4321 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     4381 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     4441 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     4501 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     4561 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     4621 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     4681 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     4741 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     4801 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     4861 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     4921 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     4981 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     5041 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     5101 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     5161 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     5221 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     5281 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     5341 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     5401 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     5461 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     5521 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     5581 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     5641 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     5701 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     5761 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     5821 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     5881 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
     5941 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
     6001 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
     6061 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
     6121 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
     6181 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
     6241 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
     6301 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
     6361 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
     6421 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
     6481 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
     6541 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
     6601 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
     6661 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
     6721 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
     6781 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
     6841 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
     6901 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
     6961 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
     7021 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
     7081 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
     7141 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
     7201 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
     7261 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
     7321 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
     7381 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
     7441 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
     7501 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
     7561 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
     7621 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
     7681 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
     7741 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
     7801 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
     7861 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
     7921 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
     7981 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
     8041 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
     8101 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
     8161 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
     8221 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
     8281 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
     8341 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
     8401 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
     8461 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
     8521 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
     8581 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
     8641 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
     8701 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aat
//
LOCUS       DACDID010000111         8731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDID010000111 DACDID010000000
VERSION     DACDID010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.112"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..499
                     /locus_tag="IV236_002052"
     CDS             <1..499
                     /locus_tag="IV236_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8772229.1"
                     /translation="HSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQVVI
                     PVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKANPT
                     NLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLASRK
                     SKSAY"
     gene            821..1048
                     /locus_tag="IV236_002053"
     CDS             821..1048
                     /locus_tag="IV236_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8772230.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1171..1497
                     /locus_tag="IV236_002054"
     CDS             1171..1497
                     /locus_tag="IV236_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8772231.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1565..2278
                     /locus_tag="IV236_002055"
     CDS             1565..2278
                     /locus_tag="IV236_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8772232.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2588..3439
                     /locus_tag="IV236_002056"
     CDS             2588..3439
                     /locus_tag="IV236_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772233.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3560..5425
                     /locus_tag="IV236_002057"
     CDS             3560..5425
                     /locus_tag="IV236_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772234.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5473..6882
                     /locus_tag="IV236_002058"
     CDS             5473..6882
                     /locus_tag="IV236_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8772235.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            6990..7691
                     /locus_tag="IV236_002059"
     CDS             6990..7691
                     /locus_tag="IV236_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8772236.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7850..8329
                     /locus_tag="IV236_002060"
                     /pseudo
     CDS             7850..8329
                     /locus_tag="IV236_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2931 a         1452 c         1936 g         2412 t
ORIGIN      
        1 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
       61 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
      121 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
      181 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
      241 tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa
      301 tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
      361 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
      421 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
      481 gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag
      541 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
      601 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
      661 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
      721 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
      781 ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag
      841 agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt
      901 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt
      961 ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca
     1021 tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa
     1081 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     1141 agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt
     1201 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct
     1261 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     1321 acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg
     1381 gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     1441 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     1501 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     1561 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     1621 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga
     1681 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
     1741 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
     1801 gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
     1861 aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac
     1921 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
     1981 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
     2041 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
     2101 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
     2161 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat
     2221 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
     2281 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
     2341 aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc
     2401 tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa
     2461 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
     2521 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
     2581 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
     2641 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga
     2701 gatttcgcca gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca
     2761 attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt
     2821 cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac
     2881 cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt
     2941 attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
     3001 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
     3061 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
     3121 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
     3181 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
     3241 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
     3301 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
     3361 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
     3421 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
     3481 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
     3541 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
     3601 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
     3661 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
     3721 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
     3781 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
     3841 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
     3901 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
     3961 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
     4021 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
     4081 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
     4141 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
     4201 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
     4261 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
     4321 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
     4381 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
     4441 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
     4501 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
     4561 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
     4621 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
     4681 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
     4741 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
     4801 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
     4861 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
     4921 caatcaatta gaaaacaaac atgcgaatgc aggaaaaccg attacttcag cacggcatac
     4981 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
     5041 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
     5101 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
     5161 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
     5221 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
     5281 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
     5341 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
     5401 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
     5461 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
     5521 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
     5581 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
     5641 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
     5701 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
     5761 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
     5821 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
     5881 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta
     5941 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
     6001 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
     6061 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
     6121 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
     6181 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
     6241 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
     6301 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
     6361 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
     6421 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
     6481 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
     6541 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
     6601 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
     6661 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
     6721 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
     6781 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
     6841 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
     6901 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
     6961 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
     7021 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
     7081 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
     7141 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
     7201 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
     7261 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
     7321 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
     7381 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
     7441 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
     7501 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
     7561 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
     7621 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
     7681 atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     7741 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca
     7801 cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg
     7861 aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac
     7921 gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga
     7981 tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat
     8041 cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg
     8101 cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt
     8161 cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac
     8221 aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga
     8281 agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa
     8341 tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc
     8401 agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag
     8461 catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag
     8521 ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa
     8581 aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt
     8641 acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca
     8701 ctgaattttc ttccagcagt gacgattcaa g
//
LOCUS       DACDID010000112         8483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDID010000112 DACDID010000000
VERSION     DACDID010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.113"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..857
                     /locus_tag="IV236_002061"
     CDS             30..857
                     /locus_tag="IV236_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772237.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1130..1900)
                     /locus_tag="IV236_002062"
     CDS             complement(1130..1900)
                     /locus_tag="IV236_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8772238.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2118..3185
                     /gene="ulaG"
                     /locus_tag="IV236_002063"
     CDS             2118..3185
                     /gene="ulaG"
                     /locus_tag="IV236_002063"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8772239.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3203..3664
                     /locus_tag="IV236_002064"
     CDS             3203..3664
                     /locus_tag="IV236_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8772240.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3684..5168
                     /locus_tag="IV236_002065"
     CDS             3684..5168
                     /locus_tag="IV236_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8772241.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5211..5510
                     /locus_tag="IV236_002066"
     CDS             5211..5510
                     /locus_tag="IV236_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8772242.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5525..6163
                     /locus_tag="IV236_002067"
     CDS             5525..6163
                     /locus_tag="IV236_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8772243.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6168..7028
                     /locus_tag="IV236_002068"
     CDS             6168..7028
                     /locus_tag="IV236_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8772244.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7018..7728
                     /locus_tag="IV236_002069"
     CDS             7018..7728
                     /locus_tag="IV236_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8772245.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7960..8394
                     /locus_tag="IV236_002070"
     CDS             7960..8394
                     /locus_tag="IV236_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772246.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2803 a         1331 c         1815 g         2534 t
ORIGIN      
        1 aataattttt taaagggaag gaaattgttt tgattaaagt agatggagca ttactaaaag
       61 aataccgaaa gaaaaagggg ttaacacaag tacaattagc agaaggcatt tgtgaacaag
      121 caactgtcag caatatcgaa aacaagaata tgtcgccagg catcaatatc ttaagtgcgc
      181 tttgcaaaaa gctgaatgtc gagatctccg atatattagt agaaacagaa gaagacaaaa
      241 tcaggaaaaa aataaatgag attcaatcat tgaattctgt atcaaaatac caagaatctt
      301 ataaaatttt acaaacgatc gatcaggaaa aagtaagtaa ttctgatctt ttaaatcaaa
      361 ttttatttta tcaaggactg cttgatcatc tttatgcgaa aaaaccagat gcagcattat
      421 tttattttaa tcaagtgttg gaacaaacga ccgaaacaga tatccatcac ttacaagcag
      481 ctgcaaacgt ggcaatgatc tacttgtcaa aaggagaatt agattttgcg aaagtttatg
      541 tggagcgtac acttaaaata ttgagtgaga ctgattttga taatctgatg gtctgcattg
      601 tatattatga tatcgccacc tattaccgta aaatcgagga ttatgataaa gcaatccaat
      661 tatgtgagaa gggtattgag tataataaga aacataaatc gacttatgca ttggaatact
      721 tgctttatga aattgcttct tgccataagc aattaggaga agatgattat ttagaaagat
      781 atatggacgc aaaaaaaata gcgcgtttca acggaaatga ttacgcggta aaggtgattg
      841 aaaacgacct gaaataaagt ggttgtgaaa tcagtgttct gatttgaata ccgtttattc
      901 gaaaaaagga ggtgtgactt ttgtcatacc tccttttttc gatattgttt gattcctatt
      961 taggcaacat ataacggtta taaaagaagc aatagattat gatggtatcg attggatgga
     1021 tacatcttgg gattccttta tcaatttgag cgtagcgtgt ttttcattta tcgataaatt
     1081 tgttattcat ttttttgata atcctattta catatttacg tcctggttct taaaatgaat
     1141 ctacttgaat ggtttttatc cccgtatttc tgatctcctc taatactttt tcagaacaaa
     1201 atttatcagt gatcaaataa tcaaagactg aaatatcagc aatcttgaac tttgatgtca
     1261 gtccgatttt tcgatgatca gcgactaaaa tcttacattt gattgtcttg ttgaccatca
     1321 tttcgttgat cgttgattca tttaatatct ttgttgtgac cccatgtact acatcgactc
     1381 cgctacagcc aatgatacaa atgtctgcat tgactgaaga aatcgtattt aatgcgatgt
     1441 cgcccactag tgcttctttt ggaaagcgca tctctccacc agttaaaata taactacaat
     1501 tatctttgtg cggagagtaa ttgattttca cgttgtttgt aatgactgtc aaatggttag
     1561 attgaagaaa atccagtgtt ttcaacgcag tcgagctaga attgataaac agcgtatcat
     1621 aatcgttaat gtatttacta gcttctctgg caatcgcttg tttaatcctt tcaggaccag
     1681 attgatcaaa atcagtctgg tttttgtaat tataagaaca ataaccgtaa ttgcgtatga
     1741 tctcgttttt ttcttctaat aaatttaaat ctcttcgtat cgtactaata gatacatcaa
     1801 gggattcagc taattcgaaa gttgatagtt gttcagtctg ctgaagcagt tccaaaattt
     1861 ctttttgacg tttttcaata ttgacaagtg agtttttcat gacttttgcc tcctgaggga
     1921 catcttgatc catggattta tttgactagt tgttcattta aacattcata tctgtcttta
     1981 ttatactgaa acaatcattg attactgaaa gaaaatcata taaaaaacaa acggtcatat
     2041 aaaagggttt tcatggatgc tgatacacat taatatttac ttgtaagttg gaacaacgat
     2101 atggggggag caagcaaatg agtaaaactg tcaatgaagt aacaaaagaa aagtggttag
     2161 tagaaacatt tcctgagtgg ggcacatact tgaatgaaga aatcgaaaca gaagtagtgg
     2221 atcctggaac ttttgctatg tggtggctgg gctgtacggg aatttggctg aaaagtcatg
     2281 aaggaacgaa tattttatgt gatctatggt gtgggacggg aaaacgtagt catgggaatg
     2341 gcatgatgaa aaaaggccat cagatgatgc gtatgagtgg cgttcaaaat atgcagccta
     2401 atttacgtac tcagcctttc gtaatcgatc cttttgcgat aaaaaatgta gacgctttaa
     2461 tcgttacaca tatccattcc gatcatctag atatccatac agccgcagca gttcatcaaa
     2521 attgtcctga agcaacattc attgggccaa aagaagtggt tcgcacgtgg caaaaatggg
     2581 gaattcctga agaaaaaacg attacagtaa agccaggtga tgcagtcaaa atcaaagaca
     2641 tcaatgtagt agctttagaa gcgtttgatc ggacagcgtt ggtcacttgt gaagatccag
     2701 aagaggtatt aaaaggaaaa atgcctcagg agatggatca aattgcagta aactatttat
     2761 tcgaaacatc aggagggaat ctttatcatg caggagattc acattactcc aatttatttg
     2821 ccaaacatgg taatgaacac aaaatcgatg tctgtttagg ggcctatggt gaaaacccac
     2881 gagggataac agacaaagtt acttctgttg atatgctgcg catggcggag tcattgaatg
     2941 caaaagtagt gattcctgtc cactacgata tttggtctaa ttttatggct gatccaaaag
     3001 agatcactga aatttggaaa ttcaaaaagg atcgcctgaa gtatggattt aaaccttata
     3061 tctggcaagt aggcgggaaa ttcatctatc cgacaaacaa agatgatctt gagtttaatt
     3121 tttatcgagg atttgaagat gtcttctcga ttgaaaatga tacaccgttt ccgtcgtttt
     3181 tataagaaag aaggggagtt tcttgctgaa atactttttt gaccatcaat tgattcatta
     3241 cccagataaa gaaatcacgg attggcgtga agctatcgcc gaaagctgtc ttgtattgat
     3301 cgataaggaa attattgatg aaacgtatgt ggaggagatc atccattgtg tagaaaaata
     3361 cggtccttat attgtgatcg cacctcaagt agcaatgccg cattcttcag aaaaaagtga
     3421 tggtgttttt ggaacagcta tttcatttac caagttgaaa cagccaatcc gttttgaaag
     3481 tgaaggggag aatgaggaga aaacggctag tttgtttttt acactcgcag caaaagatcc
     3541 agaagaacat ctagaaaata tccaaaaatt atccgaatta ttaatgacag atggattggt
     3601 tgagcgtctc atctctacga actccatcga tgattaccaa gttgtgatgg cagaatataa
     3661 ttaattgata gaggagaaaa aagatggggg aatttttgtt aagcatttgg acgtattttg
     3721 caacaaatat cttgacgcag ccagcatacc tgattggttt tatcgtgtta ttaggctata
     3781 ttttgctgaa acgcccgctt catgaatgca tcgctggatt cttaaaggca acggtcggtt
     3841 actttatttt gactgttggc tctggtggtt tggtcaataa ttttcggcca atcttggttg
     3901 gactaaagga acggttcaat ttggatgcca tggttacaga tccttatttt ggacaaaatg
     3961 ctgtggatgc tggattaatg gagacatttg gccgtacctt tggagatgtg atgattttat
     4021 tactgattgc atttgtttta aatatcttat tagtccgttt tcagaaatat acgaaactgc
     4081 gttccgtttt cactaccgga aacgttcagg ttcaacaagc agctaccgct ttttggattt
     4141 tactgttttg ctttcctgat ctaggtagga tacaagcatt gatttttatg ggactgattt
     4201 taggttgtta ttggacagta gcttctaact taacagtagg aatcacgcag gaattgacag
     4261 aaggtgccgg atttgcagtg gcacatcagc aaatgttcgg aatctttata tttgcaaaat
     4321 tagctgagtg gatgaaaaag cgggatgaaa agaaaaatag atcaatcaag caagacaaaa
     4381 aaattgaaga tatcaaatta ccaggattct tgtcgatttt caatgagaat atggttgcta
     4441 cttcgttact catgctattt ttctttggga tcattctgat tgtactaggc aaagattatt
     4501 tgattcaagc gcaattcatg caagaagggc agagtttctt attctacatt atgacgactt
     4561 ctttaaattt tgcggtatat ctggcgatct tgcaattagg tgttcggaca ttcgtcgatg
     4621 aactaactca atctttccaa ggaatctcaa acacgatttt accaggagct gttccaggaa
     4681 ttgacgtagc tgctactttt ggctttggtt cgccaaatgc ggtaacgatt gggttcttgt
     4741 ttggtgcttt gggacagttt ttgatgattg gtttactgat tttattgaaa tcaccaacga
     4801 tcgtcatagc cggtttcatt ccgttgttct ttgataatgc cgttatagca gtttatgcca
     4861 ataaccgagg tggattcaaa gccgcttgcc tgttcccgtt tctttcagga atcatacaag
     4921 taggcggttc agcattattc gctacatgga ttgggatgtc aacatacggt ggttatatcg
     4981 ggatgttcga ctgggcaaca gcttggccat tgtttacagt aatcatgaaa gtcctaggtt
     5041 atgctggaat tgccgtagtt gtcattggtt tattggctat tccgcagtta caatatcgga
     5101 aaaatcctga tggttatttt ttgattgtag aagactatga cacatatgca gaaaagcaga
     5161 gagtataaaa gaaaatctga ttagacaaca ctcataatgg agggattaag atgagaattt
     5221 tagtatcgtg tgcaaatgga tcaggaacaa gcttgatgat gatgagaagt gtggaaaaag
     5281 caatgaaaga actaaacgta ccaattacca aaatccatca ttgtgccatt tcagagggaa
     5341 aaagttcagc tggccaatac gatgtagttt ttacgccagt caattttttg aatatgttta
     5401 ctcaggcaga gaaaaaagga gttacagtta tcggtatcaa aaatgtgatg tcagtcaaag
     5461 aaatcagtga aaaatttgct gaaacaaagt ggtaccaaaa aaacaagtaa aggaaggaac
     5521 taagatgagc aggccgaatt tgcaaattgc gttagatcac aatgacttag aacatgcatt
     5581 aggagatgtt atgaaagttg gtgatgtagt agatattatt gaagtgggca caattctttg
     5641 cttacaggaa ggacaaaaag cgattcgctg tattcgcagt atgttcccag ataaaaaact
     5701 agtcgctgat acgaaatgtg cagacgctgg aggaacagtg gcgagtaatg ttgcaaaagc
     5761 aggagcagac tggatgacag tcatttgttg tgcaacgatt ccaactatgg aggcagcaca
     5821 aaaagaaata ggtgaattgc aagtagaact ctacggcaat tggacttttg aacaagccat
     5881 ggattggcat aacatcggta tccggcaggt aatctaccat caaagccgtg atgcgctgtt
     5941 atctggtgaa acttggggtg aaaaagattt atcgaagatc aaaaaactga ttgaactagg
     6001 ctttaatgtt tctgtaactg gtggattaaa tccacacaca ttacacttat ttgaagggat
     6061 cgatgtgtat acctttatca ctggtcgtgg tattacagca gcaaatgatc cgatgaaggc
     6121 cgcacaaaac ttcaaagatg agatcattcg tatctggggg taaggaaatg gctcaaattg
     6181 ggatttatga aaaagcgtta cccaaagata tttcatggaa agaacgtttt tcccttgtaa
     6241 aagaaatgga cttcgatttt atcgagatgt cgattgatga gacggatgag cggttagcac
     6301 gattagattg gtctgaagaa aagatagctg agctgagaga agagatgttc agctccggtg
     6361 tacgtatcca ttcgatttgt ttaagtgcgc atcggcgatt tccgtttggt tctgctgatc
     6421 cagaaaagaa aaaagcagcg aaacaactaa tgaaaaaagc aatccatttg gcgcataatc
     6481 taagcgtcaa agtaatccaa atagctggat acgatgttta ttatgaagag aaatcaatga
     6541 attccagaga agactttatc gaaggaataa aagaaagcgt aaaggaagct tctaaatatg
     6601 gcatcattct ttccgtggaa atcatggatg atccatttat gaactccatt tcaaaatttt
     6661 tagaaatcaa agaacagatt cattcacctt ttctacaagt atatccagac ctaggcaatt
     6721 tgtccgcatg gccggaaaat aatccggctc gagaacttga gaaagggatc gattatatca
     6781 ctgctatcca cttaaaagat acttaccctg taacaaaaga atattcaggc cagtttcgtg
     6841 atgttccttt tggtagcggt tgtgttgatt ttcttggatt attaagacat ttgaaacgtc
     6901 tagattatga tggaacattt ctgattgaaa tgtggagtga aaaaagccaa gactttcgaa
     6961 gagaaatcca gcaggcaaaa gactacttaa gttctaaatt gaaggaggct ggctatgatg
     7021 tcttgtgaag aaaaaattgc agcaatgaag caacgtgtcc ttgaagcaaa tctggcattg
     7081 ccgcgatacg gattagtcaa attgacttgg ggcaatgtga gtgaagtgga ccgtgagcta
     7141 ggagtcatcg tcattaagcc aagcggtgtc agatatgaat gcatgcaagc agatcaaatg
     7201 gtggtaacag atttgtcggg aaatataata gaagaagata gcttaaaacc atcttcagat
     7261 ctccccacac atgttgtttt gtatcaaact tttgaagata taactgctat tactcatact
     7321 cattccacgc actccgtcat gtgggcgcaa gcaggacgcg atttgccagc ttatggcacg
     7381 actcatgctg atgcttttta tggtaaggtt ccttgtacac gccaattaac gaaagaagaa
     7441 gtaagagaag cttacgaagt tcatacaggg aatgtgattg tcgagacgtt taaggaaaga
     7501 aaattagatc caaacgaagt ccctggagta cttgtctatg gacatggacc gttcacatgg
     7561 ggggattcac ctatgaaagc agtagaaaac agtttgatct tagatgaaat ttgtctgatg
     7621 gccaaagaaa acgagctgat caaccccaat atatgtgaaa tcccgcaata tttactagat
     7681 aaacattatt ttcgcaaaca tggaaagtat gcatactacg ggcaatagag atgctagggt
     7741 aagggtagaa aattaaaaga taacacttga tagagagaaa cagaggttgt gaagaaaatt
     7801 tatcacaacc tttttattta tcaaaaagta tgcattctaa aaggaaagag gaattttaag
     7861 gaatcgagag aatctttaca ggagagttat atgtgataga ttaaatagtc aaagaactgt
     7921 attattagag ggagaattgt ataagaaagg aagaaaaaaa tgaatattga ttcccttatg
     7981 atcgatgaga tttctgaatt atataagagc gttggttgga cgaattacac aaaagacact
     8041 gcaaggttag ctagagcatt tgaacaatct gaatctctga taaagcgtaa tggggaaggg
     8101 aagattatcg gaatcgttcg ttggatcact gattttgcga cgatcgcatt tattcaagat
     8161 atcctcattc atccgaggta tcagcgccaa ggaataggca aagtgctttt gaatgaagct
     8221 ttggagaaga tcactagtta tggaccagtc caaatcgaat tattgacaga tgatactgag
     8281 aaaacaaaaa aattttatga atcagtagga tttgttcaag taaaggaaat gggtggtatt
     8341 tcttatgtca tagatactag aagaaaaaag gtgctttttg gtaatgacaa ataacatata
     8401 ataacgtaca aaagaaaaaa ttctagctta gttctttaga aaaacaagag gatagactta
     8461 gtatcactca atctatcctc caa
//
LOCUS       DACDID010000113         8302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDID010000113 DACDID010000000
VERSION     DACDID010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.114"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(528..2159)
                     /locus_tag="IV236_002071"
     CDS             complement(528..2159)
                     /locus_tag="IV236_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8772247.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2173..3846)
                     /locus_tag="IV236_002072"
     CDS             complement(2173..3846)
                     /locus_tag="IV236_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772248.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3859..4176)
                     /locus_tag="IV236_002073"
     CDS             complement(3859..4176)
                     /locus_tag="IV236_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8772249.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4201..5601)
                     /gene="lacG"
                     /locus_tag="IV236_002074"
     CDS             complement(4201..5601)
                     /gene="lacG"
                     /locus_tag="IV236_002074"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8772250.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5838..6623
                     /locus_tag="IV236_002075"
     CDS             5838..6623
                     /locus_tag="IV236_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8772251.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6626..7318
                     /locus_tag="IV236_002076"
     CDS             6626..7318
                     /locus_tag="IV236_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8772252.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7592..7963)
                     /locus_tag="IV236_002077"
     CDS             complement(7592..7963)
                     /locus_tag="IV236_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8772253.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2565 a         1818 c         1420 g         2499 t
ORIGIN      
        1 ttttatacac tttacgttga aaacagagtg ggaataaaga gccaacataa tatggagggg
       61 gaatccctat aaatcaacat tcttcaatag ctaaaatgct tgattttcat taaaaataag
      121 atggttccct accaaaagag actataatat aagttaattt gtttcattct tatcataaaa
      181 agaagttctc gaaaaacgaa ctgttttccg agaacttttt tctatccatc taagtgctca
      241 aatcatattc acgacggaat tctaaggtta ccctttctta aacaaacttt atgttatctg
      301 cacaactttt tgcaattttt tcattgcttc ttacgaaatc gttgaactct ctttagcata
      361 atacaaagtg tagatgaagt ctggtgcttt actcgttttg gtaaaaatga ctggaaatcg
      421 tttctggtta tttttggcaa aggcgaggtg caggttcgat tcctgccttc tacgctaacg
      481 atgtggctga agggaacctc cgatcttgaa tcgaaacgat ccgcagtcta ttgtttcacc
      541 aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag ccttgctata aaccccaatt
      601 tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa tttttgtcca ttcacttgtt
      661 ttaacaaccg cagtatttag ttccgctcca ccataatttt tgatatataa ctgattagta
      721 tcaaactctc ccccagacat tacataggca ctcaatgtgt aagttccttt gcttatgcca
      781 ctaacatttt gataagctga tgcctcgtat gcttgatcct gccaaaaagt tagtttgtag
      841 tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct tagcggttcc ttgttgaaac
      901 caggtttgcc aattggaagg actggtcgtt ggtccatcgc tttcgaaact tgcattttta
      961 actagattta tttgcgcccc tgcttgttta aacgcacgca agcttggcaa ggcgtttcca
     1021 gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca tagtggattg aacactgaga
     1081 taattcatac ctgcttgtgt tgcccaactc acatttccgt tgtaccaaag aggttcccaa
     1141 tagaagaagc ccgccccgcg attattcggt actgcctgaa tccgatcggc aagatccagc
     1201 aaatactctg cttgtcctgt cggtgttggt tcatatcccc ccgcagctgc ttcggcttca
     1261 ccaaaagcat ttggcatagt atcggcattc gctgttgtat gcccataagc tgtttcaact
     1321 acgatcactt ctttaccata ccgttggctg atcgcattca tattggtttg aagttcattc
     1381 attgaaccgt gccaatacgg ataatacgac actccaatta aatcaaatga cacacctcga
     1441 ctcgtgattt catcaaacca ccaagtaaat gctcctgtat cgccaccatc agctaagtgg
     1501 agcattactg gagttgtttt aggttgagtg tctcgaacag cttggatacc agacttcaaa
     1561 aaagcagcca atcgatcaaa ttctcccccg ccttctcccc aagacttgcc ataaggccag
     1621 agcattccgc tattcagttc attgccgatc tgaaccatat caggataaac gtccagctgc
     1681 ttcatttccg acagaacgtt ctttgtatag tcgtacaatg ccgtattcat ttcttcaaag
     1741 gtcagacctt gccatgcttt aggaagattc tgtttcccag gatcaaccca aaaatcactg
     1801 tagtgaaaat ccagcagcac cttaagtccc agattttttg ctcgttttgc aaggtcaatc
     1861 gtcgtttcca gatcatttgt ccctgctcca taactttctc cattactatt ataaggatcc
     1921 tgccacaaac gcagtcgaac atagttgaca ccattatttt ttaaaatctc taaggcatct
     1981 tttgaaatat cgtcttcata atattttgca ccagattttt ccatatctga caggatcgaa
     2041 atatctgctc cgcgaataaa ttcgctggca gatgcttcaa cagtttttga aacagccccc
     2101 aaagccccta acacagctgc aaataaaaca gctttacgca accaaattcc cattttcatt
     2161 atggctcctc ctttattgaa attggttcag aataaactga acagcttttt caggatctct
     2221 cgttaatccg atatattctg ctccttttgt cgcaaccagc ttgattccta agcgatcggt
     2281 gtcttctttg atatcatcat agtaagaatt gacttgcggt gctaagacca ccatatcaaa
     2341 atcctgcagc atttcgtaat gagaaccata agcacccgcc attgcactga tcggtgcttt
     2401 cgattcagat gcaccttttt ttaaagcatt agctaacatc gcacttgtcc ctgcgccagc
     2461 acaaagtacc aacacattga tttctttatt tgcatcgctg ataacttgac tcgctgccac
     2521 aggtacttcc tcacgtacat cggcagaagc accttcctcg gttgcgatca cctctgcttt
     2581 ttgtgcttct ttctctacta agacagcatc gtatgctttg caaaatggta gatagatcag
     2641 gaagtcaacg acgattagaa ccgctgctag aacaaaagct aagataccca tgcccgttcc
     2701 tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa acgtacataa agctgttcat
     2761 gcccaataca tcgacaaaaa acttgaacag gcaaacgttt acaacaggtg ttaacaaaaa
     2821 tggaataaaa aaataagggt tcaaaatcat gggtgccgca aatagcaatg gttcgttgac
     2881 agcgaaactt actggaataa aggaagcttt cccaactgcc ttcaactgct tagacttagc
     2941 aaagagtagg aagataaaag gaacaaccaa tgttgcccca gttcctccca tagttcctac
     3001 aaaattccct aaacccaccg tcagtacttt agaagcttgt tcaccggaat tgaacaattg
     3061 cagattggtt tccacattgg catagataat tgccgcaatt gccggctcca cgatcgacgg
     3121 tccatgaaca ccgacaaacc aaaacagcgc catcgctccc cagataatag cgattcctaa
     3181 gtacccgtca gctgctgtaa atagcggctg cagcaccgta atgattgctt ctgcaaatga
     3241 gatttggaag atttgtcgaa tgcctaaatc aattaaaacg gccgcaaata ctgaaaaaga
     3301 gaaaggaaaa atatctcgga aggtttgcga aatcgttcca ggaacttctt taggcatttt
     3361 aatggtaata tctttcagca cacaaaactt atataaatta actgttaaaa atgcagcgac
     3421 aaaggaagcc agtagtccct ttgtccccat atattccgtt gaaaatccac cttcaatcgg
     3481 agagacgctt aacaacagga aacttaccac agaggccagc atcacagagg tggtgttgat
     3541 tactttgttt ttcggtaagc gacgattcat cgaatcagcc aagcagcgag cagtggttgc
     3601 tgaaacgagt aaccctacaa cacccatcga ataattatag actttccata gccatgtgga
     3661 gacagattca ggccattggt aaccaaaaat ctcaggaacc gcagcaatca aaatgaaaat
     3721 gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg tcacgaacgg ctcctaaata
     3781 actattacgt gagatttttt caaaaaatgg tttccctttt tcgatttgtt taatgattcc
     3841 atccatagcg ttcttctcct atccttgatt ctgtctgtac agtacaataa agtcttgaat
     3901 cagatctctc agcaataaag tggtcattaa atgatcctgc ccatggataa aaatgaatcc
     3961 catctccatc tctttcccag atgcttcttc cgctaaaatt tttgtttgcg aattatgtgc
     4021 aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca acatgttcaa agtttccttg
     4081 tcttacttct ttcaataatt tcaataatgt actgcgagcg tcgcctgaat atgcaacgat
     4141 ctcaaaacca agcatttgta attcctcttt attcatcttt tcttcctcca ttatcactta
     4201 ttaaataatc gttttggttt cactgaccaa cttgtaccag taagctgatt cttttgggta
     4261 acgcttctgg gtttcaaaat caacataaaa caagccatac cttttgttat acccatttgt
     4321 ccatgagaaa agatccataa gcgaccacaa aaaatacccc ttcacattta ccccggctgt
     4381 tatcgccttg cttaaggatt ccaaatagac tctcaaatag tcgattctcg gctgatccat
     4441 aataatgcca tcttcaaact gatctttata ccccatcccg ttctcagtga tatagatttt
     4501 gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag tcatatagac cttctggata
     4561 aatcagccag tcccaatcag ttcgaggaat tccttcttta taaatacgtt ctccgattcc
     4621 cttcacttta taaacagagg tccctttttc acccgtaccg ttatgatgga ttgcattttc
     4681 tccatcgtaa gctttgacaa aatggcattg gtagtgattg atccctaagt agtcattacg
     4741 cgtggatgct tttttcaatt caacgaaatc ttcttcagga aaatgatagc tggcctgatt
     4801 tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt gaataatagc ctaaataggt
     4861 agcatctaat aagaaacgga tcgacaatgc atcgtctaaa aaggcagcat gcttatcttc
     4921 cggcgcgtct gttgccgcat atttcgtctc taatgagtga actacaccaa tttcacccgg
     4981 aagttcgttt tctttgaaat agttcactac acgggcatga gcaaccatca tattatgaag
     5041 acaggcgacg atctttgtaa aatcatattt gattcctggt gggaaaacac ctaacaaata
     5101 ttgattggtt gcaactggat agatttcatt aaaggtactc cacaccttga cttctttgaa
     5161 ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct attgtttctc gattcaaaaa
     5221 atctccatgg tcaaacaaac gtttaggggt atcaaaatga tgcaacgtca caaacggcgt
     5281 tacgtttctt ttcttacatt cttcgaacac acgatgataa aaagcgattc cttctggatt
     5341 tggtttccca gctccatcag gaaaaatgcg gctccaagca atggataatc ttagaccatt
     5401 tacaccaaaa cgttcacaca attcaatgtc ctttgcatat tgatgataaa aatcacttgc
     5461 cggatcggga ctgaatcgcc cttgttcctc taaaaagtca tcccaagcaa cagcgccttt
     5521 gccaccttcc ttagtggctc cttcaacctg ataggctgct gtggctccgc caaaaataaa
     5581 atcttctggt aatttaagca tgtacatctc tcctcaaatc tataatcagt tttcgctctt
     5641 tctaactgat tgcattgtaa acgcatccaa aacatttgtc aacaaaaaca aacacaaata
     5701 atcataaaaa ccagaaaaca gcaataaaat ctattgtaac aaacataaaa accaccctaa
     5761 atgtttactt ttgttcgaat tagattgact gttgtccgtt tatatgatag attatcctta
     5821 aattagaggt gaagacaatg ttgaaaaaag aaagattgct tactatcgta gaaatggtca
     5881 ataaaaaagg aatccttacc gttaatgaaa tcatcaatca attagatgtt tctgatatga
     5941 ccgttcgcag agatttagat gaattagaaa aatcgggcaa gctgctgcgt gtccatggag
     6001 gggctcaaag tctttcctac actttagatc aagaactttc tcacactgaa aaagccactc
     6061 tgcagattca agaaaaaaaa gaaattgctg ccacagcagc ctccttgatt cacgagggag
     6121 aaacgatttt cttaggacca ggtacaacaa tcgaactatt ggcgacgcat ttactgaaca
     6181 agaaaattcg gatcattaca aataattatc cagtttttga tattcttgct caagcagatg
     6241 gaccagaaat tctactgatt ggcggtgatt tccggaaaaa taccggcgca tttgtcggac
     6301 cgatcaccaa cgacaattta agaagactaa agtttacaaa agcatttatc agttcaaatg
     6361 gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga aggagaagca cagcagatcg
     6421 ctctaaataa ttcccgtaag aaatatctac tcgttgataa taaaaaattc aatcgagatg
     6481 atttttatgt gttttataat ttgcatgatt tcgatctgct cattacggat cgccagatct
     6541 cgcgggaggt tcaagagcac tatgcacaat atgtcgatct gcaaattgct gattcaaccg
     6601 aaaataccac tataggagcg taactatgaa agggattata tttgatttca acggaacaat
     6661 gtttttggac tctcatctgc atgaagctgc ttggctgcat atgatacatg atcatacaaa
     6721 agatggttta tcagacgagg atattttaaa aaatattcat ggtcggacga atacggagat
     6781 tttgaaacat ttcatttcag agcatttgac cgcctctgag atcgccgtat tgtcagaaga
     6841 aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa aacgagcttc agttgacaaa
     6901 agggcttgaa gcgactcttg accagctgac tgaccaacag attccattaa cgattgccac
     6961 ggctactgtc aaagagaatg ttgcttttta ttttgatatt ttcgatttag cccgctggtt
     7021 tgactgggac aagattgtct atgacgacgg ctctttccct gggaaaccgc aaccggatat
     7081 ttttcttaaa gcggcagaca aactgagctt agctccggct gattgtcttg ttatcgaaga
     7141 tgcttactca ggattacttg ctgccaaacg tgcggggatc ggaacaatta ttgcaattga
     7201 tccattcggt aaaaacaaaa cagtatttga acaggcacag ctaaataaag atggaaccat
     7261 caaagatttt actggttttt ggacagagca cctgtctgcc acatccgtta attcttaaac
     7321 gattcaacta actaccaaag aaaagcaact gaaatgcaca cctcctttta taaaaaactg
     7381 ccagtgaaac gacatgaaag atacactgct atatccttag tgaaatatag cagtgtatct
     7441 ttgacttttt cattctttta accatatgac gcgttcatct ctatctataa ttaacattaa
     7501 aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa atcatcagga gtactttttt
     7561 gatatacttt tattaatgct gggcgtaatg gttaaaagat ttccactagt tcctctccac
     7621 caagctgagt tgaaaaatgc ttatctgtaa acaacataga caaagaggaa ataaacgttt
     7681 ccgtatcttt ctctgtccca aggatattaa ttaattttcc tactaggtaa gtatcattaa
     7741 acttaagatc tgccttcaca tcaaaagttt tgataaaagc ttttatcatg cgtacaattt
     7801 tgtctttatc gccataattg atttcaccac tcatatcaat atagtcacta atccctgaag
     7861 aaataatggc aactgcttct tctttcgcct tatcagataa ttcatttaat gactgggatt
     7921 gattgatgac ttcttgagtt ttatctgtta cgtaatctct cattatccta tcggcatact
     7981 ccgcaaagta atcttgaaaa aattcttcta cagggtaacc aacttcgttt tcccaaccag
     8041 gagaaatttc attatcagta cttattaaat ctattaaaaa ccaacccgtt ctctgagtgt
     8101 tgattctata atatttttaa tttcctatat aaatcaaaaa tattatagaa tcaataagct
     8161 aattaagaaa caaactttag aaactctaga agaacttctg aatttatata gaagtgtgta
     8221 gctacttact aaatatacaa aagttaaaat aaaatactct ggatactctt tatactgcat
     8281 taaagttagt aatattaaca gc
//
LOCUS       DACDID010000114         8215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDID010000114 DACDID010000000
VERSION     DACDID010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.115"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..1288
                     /locus_tag="IV236_002078"
     CDS             join(126..402,402..1288)
                     /locus_tag="IV236_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8772254.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1568..2548
                     /locus_tag="IV236_002079"
     CDS             1568..2548
                     /locus_tag="IV236_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772255.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2670..4088
                     /locus_tag="IV236_002080"
     CDS             2670..4088
                     /locus_tag="IV236_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8772256.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4159..5628
                     /locus_tag="IV236_002081"
     CDS             4159..5628
                     /locus_tag="IV236_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772257.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5638..6105
                     /locus_tag="IV236_002082"
     CDS             5638..6105
                     /locus_tag="IV236_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8772258.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6141..7013
                     /locus_tag="IV236_002083"
     CDS             6141..7013
                     /locus_tag="IV236_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8772259.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7094..>8215
                     /locus_tag="IV236_002084"
     CDS             7094..>8215
                     /locus_tag="IV236_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8772260.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRD"
BASE COUNT         2807 a         1231 c         1687 g         2490 t
ORIGIN      
        1 tatcaagtaa gtctgacaag aaaggaagat tgacatgaaa aaaatgattt ttgaatcggc
       61 tacactagac tagacagaaa aaataaggtg tgtaaaataa atgaaaacac actggaggaa
      121 tttttatgtc aagacgtcaa cgtagaacct attcaaaaga attcaaacaa caagtcgtcg
      181 atctttatct cgcaggaaag ccccgagctg aaatcattcg agattatgag ctgactacct
      241 cggcctttga taagtgggtc aagcaagcgc aaactactgg ctcgtttcag gaaaaagata
      301 acttgatccc tgaacaagta gaactaattg cattacgaaa gaaaaataaa cagcttgaaa
      361 tggagaatga cattttaaag caagcggcgc tgatattggg acgaaaagac aagtaattga
      421 taccaataag cacaaatact caatatcagc gatgtgtaaa gtgttaaaca tctcttgcca
      481 aacctattat tatcaagcca aaccagttga agatgaatca gaactggaag aactcgttca
      541 agaagaattt attcgcaatc gaaaagctta tggtaccagg aagctgaagc agtgcttggc
      601 caatctaggt cttcaagtga gtcaacgcag aatcggccgt atgatgaaac agcgtggctt
      661 gcgttcaaca tacacattgg ctcatttcaa attccattct tctacttgta acgaagccaa
      721 gaccgccaat attctagacg gaaccttcct acaggaaaag tcattagaag cgattgtaac
      781 cgaccttacc tatgttcgtg ttgggaagaa atggcagtat atctgcctga ttcttgattt
      841 gtttaatcga gaaatcatcg gctattcatg cggggagaag aaagatgcga cgttagtcaa
      901 ggaagctttt gcaaagattc cgtactcgtt gaataatgta caactttttc acacggatcg
      961 tggaaaagaa ttcgataacc agacgattga cgaaatttta tgggccttta atatcacgcg
     1021 ttccttaagc aaaaaaggct gcccctatga taacgccgtt gcagaatcca cctataaatc
     1081 tgtcaaagta gaattcgttc atcaataccg ctttgagaca ttggctaatt tgcgattaga
     1141 gctatttgat tacgtccatt ggtggaatac catccggctg cacggcacgt taggctatga
     1201 gacaccgatc agttttcgac agcagagatt ggcgcagcga atccttgata atgagctcgg
     1261 atgtgatacc ggtggcgagg caccttaatt atttgaatga gcttctgcct tagagaatca
     1321 gattcgagga ggctcgttgt caagggcagt cggaacacca gcagcaagct tacgcaatac
     1381 cttataattt ttgtcaaaaa aagtgttgcc attccacata tatctttaaa cagctattgc
     1441 cagataaaaa taaatgatat tatatatata aatacttatc cgaaaagggg aatgagagag
     1501 tcaaggttct tggaatgtcg aatagtcgag taggtagatt ttctatctac aaccttgttg
     1561 tttaaaaatg aaaaaaattg gaattagaga agttgcgaaa gaggcaaatg tttctcctac
     1621 gactgtgtcg agggtattga atgatcgtgg atatttgagt gaggaaacaa aaagaaaagt
     1681 ttttgatgcc atgaaaaaat tagaatacta tcctaatgaa ctggcgagag cactttttaa
     1741 aaacgaaaca tataccgtag gtttgatttt tcctacaatt actaatccat ttcacgctga
     1801 gttaattcaa gatatcgagt tcgagctttc cagcagggga tataaagtgt tgctgtgcaa
     1861 tagcttaaac aatccaacaa aagaaaaaga gtacttgacg atgttgagaa agaatcaggt
     1921 tgatggaatt atagtaggaa cgcacaataa agatattgcg gattatgata ttccagggct
     1981 acctattgta gcgattgata gaagtcttgg tgaaaataca gttacagtat cttgtgataa
     2041 ttacaggggt ggggaattag ctactcaact acttgtaaat catggctgtc agaatatact
     2101 gtgtattcgg ggagatagta agataaagct acccgcgaat agtaggactt tagcatataa
     2161 agatgttttg aataaattca tgctggaacc gttagtccta gaagtaccat ttgtaaaaga
     2221 aatcaatgaa aaaaatgaaa taattaagac atatctggaa actcatccaa atattgacgg
     2281 ggtttttgca ggagatgatt tattggcttc tttggcgatt aacaacttag gaagttcggg
     2341 caaaagagtt ccaaaagagg ttaaagtgat tggatttgat ggagctcaac aaacattgat
     2401 ttataatcca gaattatcaa ctattcaaca gcctatagat caaatatcta aagtagcagt
     2461 aaaaaaatta ttgaatatga ttaagggcga aaaggagacg gatgaacttt cacttccagt
     2521 aaagctcata aaaaaaaata cagcataagt taaatcggcg aaaaattcct tcgtcgattt
     2581 aaaaatattt ttatgaaacg ggttgacata tgaaacgggt tgacatatat tagttgtgaa
     2641 gataaaaacc ttttcactgg aggaagaaaa tggagaaatt attaattaaa agcaataatc
     2701 atcaacttta ctaccaacct aaggatgttt gggtcggaga tgttatgcct tacgggaaag
     2761 aaggaaaatt ctttatttat catcaacgtg atactagaaa tccagttcct tttggagaac
     2821 cgtttggctg ggctttagca actacgaaag attttgttca ttttgaagac tttggggaat
     2881 ctcttttaag aggggatgac aatgagagag accagtttat ctacgctggt tctgtatttg
     2941 aggcaaatgg aaaggttcac gcattttata cagggtataa tcgtgaattc ttagcgcagg
     3001 gaaagacatc tcaagtgttg cttcatgctg agagtgatga ttttgttcat tgggaaaaat
     3061 cagaaacagc attggagtta gaaccacaag aagggtatga tattcgaaac tggagagatc
     3121 catttgtcgt ttgggatgat gaaaaagaag aatatttgct aattttaggg gcgcgaaagg
     3181 gtcaggataa acatctacaa accggacgtt tagtggaatt tacatcaaaa gatttaacta
     3241 attgggaatt caaaggtgat ttttgggctc cgggattgta taccatgttt gaaatgccag
     3301 aacttttcaa gattggtgac tggtggtatc ttgtctattc agaatactca gatggaaata
     3361 aaatactcta tcgaatgagt caaagtttgg aaggtccttg gattaagcct aaagatgaag
     3421 cttttgatgg acgggcttat tatgcaggta gaacagctta tgatggaaaa cgtcgagtcc
     3481 tatttggatg ggtaccaaca aaagatcaag aaaatgataa aaacaattat ttatggggag
     3541 gtactttggt tcctcatgaa gtctatcaaa aagaggattt ttcacttggg gtaaaaccca
     3601 tagacgaagt gtggaatgca ttcataaatt tacatgatat tgccgatgta gagatagaaa
     3661 cagcagacac aagagaagaa gtaacgcttg tcaaaaatag tggaactttg tttaatttag
     3721 aaacaacaat ttcatttgat gagaatctta gtgacttttc actgagatta tataaaaacc
     3781 ttgagacaga tgaatcttac gaatatcgct tcaatttaga tgaggggcgt cttacatttg
     3841 ataaaaaccc ttgttaccct tggtttcaat atatggataa aggacttatt agacctatat
     3901 ctttaaagcc taaccaatct tataaactaa aagtgattgt tgatgacaat ataatgacac
     3961 tttatattga tggaatcgct cttaacgctc gtgtacatgg ttgttttggt caagcaatta
     4021 ctattacagc aacaaatgga tcaatgactg caataaatac caagttttca aatgaaataa
     4081 agaaataata aacgtcaatc atgtggcagg tctgaaaatc aaacatgacc tgtcacattt
     4141 aaaagggagg ataaatgaat ggactacaaa aaaattgccg aagatgtatt ggaaaacgtt
     4201 ggaggaaaag agaatatctc aagcgctaca cactgcatta ctcgtttacg tttaattctt
     4261 aaagacaaaa gcaaagcgaa tatggaaatg cttgaggaaa tagaaggtgc aaaaggtgtt
     4321 cttttcaata gtggacagtt acaagtaatt ttcggaacag gtataattga aaaggtttac
     4381 aaagagttcg ttgaattgac aggagctaag gaagtatccg tttctgaaat gaaagacgaa
     4441 ggagtcaata attcaggaaa tagacttcaa cgagctttta aagttttctc ggatatcttt
     4501 attccgatca ttccagcttt tattggggca gcaatggttt taggaattcg ttcgttgatt
     4561 accactcctg gaatgtttgg aatggatggc tcattcgctg atcacaatgt attcctaaat
     4621 gatttatcca aatttctggg tattattgca accacatttg attatttacc agttttagtt
     4681 atgtattctg caacaaaacg ttttggcgga aaccctattt taggattact tgtaggattt
     4741 gttatgattc atccaggact agccaatcgg aatgatttcg tcatgggaac tataaagcct
     4801 gattattggc atttatttgg attaagcatt ccagcagttg cgtttcaagg gggtgttttt
     4861 ccagcaattt tgacttcttg gtttttagca aaagttgaga aaattacaaa taaatattct
     4921 cctcaagtac ttgcttacat tttagtccca actgtgacaa ttgttcttgc caactttgca
     4981 ctatttttag tgtttggtcc attggggaat tttatcggta atgttttagg tggaatcatt
     5041 gatttcctat atatgaagat gggtgccttt ggtgcttttg tttttgcagg cgcgttacaa
     5101 ccattagttg taacaggaac acaacatgct attcaaggta ttgaggcaaa cttagtcgca
     5161 acaactggat tcaattatat ccaacctatt tggtcagttt caattattgc ccaaggtggc
     5221 ggttgtattg gtatgtattt actctttaag aaaaaatcta aagatagaga tattgcgatt
     5281 tcaagcttta ttccaacact ggtaggtatt actgaaccgg ctatctttgc tgttaatttg
     5341 aaatattcaa tcgttccttt tgtttgctcg tttattggtg ctggttttgg tggagcattt
     5401 atgaaaattt ttgatgtaaa agccattgga caaggattga ctgtattgcc aggtctgact
     5461 attgctaatc cagttcttcc ttatatattt ggtaatctca tagcattcat tttaccaatc
     5521 atttttattc tcgcttatga taaatataaa gtgtttatcc ctgaagctaa acaccgtaaa
     5581 tttgcagaaa aaactgactc aaaaaaattg ccagttcaag taaagtgaga tgagagcatg
     5641 ttaaattttt ttagaaaaaa tgaaataagc gttgtagcac ctgcaaccgg tgtttttgaa
     5701 aaactggaga aattaagtga tccagtcttt tcaaaaggaa tgatgggaca aggatttgca
     5761 attgatgcaa aaaacgaaac gattgtatct ccaatcgatg gttttgtttc aagtattttt
     5821 cctacaaaac atgccttagg aataaaaaca aaatcaggaa ttgagatact tgttcatgta
     5881 ggaatagata cagttgagct aaatggtgag ggatttgata ttaaagttca agaaggacaa
     5941 aaagttaagg ctggggatat tcttatgacc attaattttg tagtttttga aaagaatcac
     6001 aagactaaag atgtcattgt tgtttttcca gactaccaag acgaaatttt cattccgaaa
     6061 cttgaagaag taaaacaagg acaagaattg tttgttcttg gctaatgata agtaattaat
     6121 aatagaatcg aggaaaatgt atggtttttg gggctattga agctggtgga acaaaatttg
     6181 tttgtgccat tggagataaa gatttgaaca tatttgaaaa agtcagcttt cccacgacta
     6241 cacccaaaga aacaatgaat ttagttttag atttttttaa tcatcataaa gatgatttag
     6301 aaggtatcgg agtgggatcg ttcggtccta ttgatattca ttgtgaatct aaaacgtatg
     6361 gcttcattac atcaacgcca aaacttgctt ggcaggattt tgattttatt ggaagtttaa
     6421 aaaagcaact taatattcca actgcttgga cgactgatgt aaatgcctct tgctatggtg
     6481 aatatgtttc tggaaatggc agaggccttt caagtgttgt ttactatacc gtaggaacgg
     6541 gcataggagg tggcgctatc caaaacggaa acttcattga aggttttagt catccagaaa
     6601 tgggacatat gcttgtaaaa cgacacaata aagacaactt ttctgggaca tgtccattcc
     6661 acggagactg tttagagggc ttagctgctg gtccggcaat cgaaagaaga tcagggaaaa
     6721 agggacaaga agtgactgaa aatgacgtta cttgggaaat agaagcttat tacattgctc
     6781 agtgtgcata taatacaaca ttgatgttat cacctgatgt cattatattt ggtggcggag
     6841 taatgaaaca aaaacactta aaaaagaaag tacaagataa gtttattgag ttgatcaacg
     6901 gatatgtcgc tattcccgat gttgacaatt atattatgac accaaaattg ttagataatt
     6961 ctggtattgt aggttgttta gctttagcaa gagacgttaa agaaaaatct taatttggaa
     7021 aaaagatcgt ctcgactatt tgtttggttg atgcgatctt tttttcaatt agtaattgaa
     7081 gggacgccta aaagtgaaaa aaaaaatgaa agatgaagta ttagagtata tcaaaaataa
     7141 tttaaagtat tacgattttt ctgctcaaga tattgcaatg aaattttgta ttaaaagaaa
     7201 tgtcgcttcc cactatttga atcagttatt ttcagatgga aaattactaa agaatgattc
     7261 tgttagacca gtaatgttca agtataatca acaaaagcca aaagactgct tttctaagtt
     7321 tattggagca gacatatctt tgaaatccac tatagataaa tgcaaagcta ctgtaatgta
     7381 tccccctaat ggcctcccat taataataaa aggaaacagt ggcgttggaa aaagtttttt
     7441 agcaagtttg atctatcagt atgctcttga tagaaaagta attcataatg acgcaaaatt
     7501 tgttgttgtg aattgtgcag attatgcaaa taatccagag ctattgtcag cagttttatt
     7561 tggatataaa aaaggagcct ttactggggc tgaaaaagat actgcaggga tgctatcttc
     7621 tgctaatgga ggataccttt ttttagatga ggttcacaat ctaagtgcag aaaaccaaga
     7681 gaaattgttt cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa gtgtgcaatg
     7741 ggagtatgca aatgttcgcc ttatcttagc cacaacagaa gataaaaata cctctttgtt
     7801 agcaactttt agaagacgca tcccagctga aatcactctg ccagattaca ataatagaag
     7861 tacatctgaa aaaattcaat tattatttga atttcttaaa gatgaagcaa ttacgattga
     7921 ttcaaaaata ttttgttcga ttgaactttt tactgatctt ttatcgaaaa catttgaagg
     7981 aaatgttgga gaattgagga atgaaatcaa gcttctttgt gcagaaggtt atttaggaaa
     8041 taagagaaag tcttcaattt atttagggga caaactagac agcggttttt ggatagatcc
     8101 agaaattagt atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa ttgatttgat
     8161 tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa gagat
//
LOCUS       DACDID010000115         8051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDID010000115 DACDID010000000
VERSION     DACDID010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.116"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IV236_002085"
     CDS             94..1248
                     /locus_tag="IV236_002085"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8772261.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IV236_002086"
     CDS             complement(1270..2022)
                     /locus_tag="IV236_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8772262.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IV236_002087"
     CDS             complement(2386..5052)
                     /locus_tag="IV236_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8772263.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTYDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IV236_002088"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IV236_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8772264.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV236_002089"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV236_002089"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8772265.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2332 a         1677 c         1443 g         2599 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatacgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata t
//
LOCUS       DACDID010000116         8017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDID010000116 DACDID010000000
VERSION     DACDID010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.117"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            195..896
                     /locus_tag="IV236_002090"
     CDS             195..896
                     /locus_tag="IV236_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8772266.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1505..2521)
                     /gene="tsaD"
                     /locus_tag="IV236_002091"
     CDS             complement(1505..2521)
                     /gene="tsaD"
                     /locus_tag="IV236_002091"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8772267.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2518..2982)
                     /gene="rimI"
                     /locus_tag="IV236_002092"
     CDS             complement(2518..2982)
                     /gene="rimI"
                     /locus_tag="IV236_002092"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8772268.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2989..3531)
                     /gene="rimI"
                     /locus_tag="IV236_002093"
     CDS             complement(2989..3531)
                     /gene="rimI"
                     /locus_tag="IV236_002093"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8772269.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3515..4339)
                     /gene="tsaB"
                     /locus_tag="IV236_002094"
     CDS             complement(3515..4339)
                     /gene="tsaB"
                     /locus_tag="IV236_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8772270.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4428..5408)
                     /locus_tag="IV236_002095"
     CDS             complement(4428..5408)
                     /locus_tag="IV236_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8772271.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5432..6916)
                     /gene="galT"
                     /locus_tag="IV236_002096"
     CDS             complement(5432..6916)
                     /gene="galT"
                     /locus_tag="IV236_002096"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8772272.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6928..7917)
                     /gene="galE"
                     /locus_tag="IV236_002097"
     CDS             complement(6928..7917)
                     /gene="galE"
                     /locus_tag="IV236_002097"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8772273.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2262 a         1713 c         1407 g         2635 t
ORIGIN      
        1 ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca ataccattgt
       61 atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt tctaatgttg
      121 gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag
      181 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
      241 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      301 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      361 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      421 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      481 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      541 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      601 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      661 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      721 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      781 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg ttagattatg
      841 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      901 aacaaaaata agcagtgacc ctgataaata tcagggtcac tgcttatttt cctccctaaa
      961 acttctgaaa gatccggcag ttttagatca ctggacaaga ctcaaagatt ttctatgtgt
     1021 gtttcagtta tgtaatattc actgtttgca aaaaatactg ttagattaac tagatgaagc
     1081 gtcttgtcct catccgatct agattattcg atattcctcg acaataaacg attactcagt
     1141 gagggcaatc agaggaacgg ataatgataa ataagctgta aaaaatagca aaataacaat
     1201 aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa cgtgtcacag ccctatttcg
     1261 attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca taaacatgag
     1321 gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct tacgaactct
     1381 tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag gtcaaaaaca
     1441 tgagaagccg tttttcctgg gctgttctta ttacttgatg cagggcagct ttttcacaac
     1501 ctctttattc agataaaacc aagcttggat cagcattcaa ctgatagcta gcaaaatttc
     1561 ttttttgcat ttccacatgt gctgctgctc caatcattgc agcattatct ccacaaagtc
     1621 gaagaggggg aatcactagt tctacctcag ggagtttagc tgccaaagca tcctgcaatc
     1681 cttcacgcaa cccttgattg gccgctacac ctccagctat aatcagttgc ttgactggat
     1741 aattttgaca agctcgaagt gttttagtaa tcaatacatc gatcacactt gcttgaaagc
     1801 tagctgctaa atcatttttg tctagatctt ctccacgctg ttgtgcatta tgcaccaaat
     1861 tgataaatgc actttttaat ccgctaaaac tgaaatcata attatcttca tggatcatcg
     1921 ctcgcggaaa atggtaatta tcttttccct gatgagctag ctgatcgatt tcttttccgc
     1981 ttgggtaaga aagacctaat actcttccaa ctttgtcata agcttctcct gctgcgtcat
     2041 ctcttgtctc cccaatgatt tcatatgatc catcttcttg catatagact aattctgtat
     2101 gtcctccgct aacaagaagt gccatcaatg gaaactggaa tggtttaacc agacgggctg
     2161 catagatatg ccctgccata tggtttacag gaataagcgg caattggtgt gcccaagcaa
     2221 aagccttagc tgcagaaata ccaatcagca acgagccgac aagtcctggt ccataagtga
     2281 cagcgactgc cgataaatct tctgccgaca caccagcttc taccagtgca tcttccaagc
     2341 ataaggtgat ttgttctacg tgatggcggc tagctacttc aggtactacg ccaccaaaac
     2401 gtttatgact gtttatttga gaagcaacga tgttagataa gatctctgtt ccatttctaa
     2461 cgaccgctac actcgtttca tcacaacttg attcaatcgc cagaatcaat tcttcattca
     2521 tttcatcgtc tccgttttta actttctgct cataatgatc gcatcttccg tcggattgcg
     2581 ataatatgat ttccgtttac ctaatatacg aaacttttct gattcataaa gttttcttgc
     2641 tgcttcatta gatagacgta cttctaaaaa aacatgcagc gaatctttta tttgttccgt
     2701 aatcagtaat tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc
     2761 aatatttgtg atctcgactt catctattac tttcgagtat ccaataaagc cacagatctg
     2821 gttatcttca gctaaaagca gataatcaga gtgaggctgt tggatgtctg ccaaaaactg
     2881 cattttcgtc caaggtgaac catgtggata agcattttcg ctaatcatcc atacgcattc
     2941 tgccaagtac gcttcatcaa aatctttttt tctgcaaatc aactaatctc actcattttc
     3001 taaaagtttt atcatatcca acgcatcttc ttttgtttca gtgtaatatc tttttttgat
     3061 cgttgttgct tgaaacccca atttccgata aagtctttgt gcatcttgat tactcatacg
     3121 cacttctaat gacatcgtct cacagcgatt cattatagca aagttctcga tttcgtcaat
     3181 aagcagcgaa ccgattcgtt tacgctgata tgccggtcga acggcaatat tcgtgatatg
     3241 gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc attcttccat tatcaaagat
     3301 acctagatat agatgaggaa gcggtgactt caattctgat ataaaggcac tttttgtcca
     3361 tggcaattcg ccattataga cttctcgttc gacatccagc agctctttga tatcttctgt
     3421 agtcaattct cttagtacgt aacgacgatc gatttgttcg actgtttttt ctgtataaat
     3481 cattttatct tgaaaaaagc ttctggcata cgctctaaat tttttcaaca taactttcgt
     3541 ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc tactcgtttt aaatagcgag
     3601 gaagaaaagc ttgtttctct gcaactggtt caactaaagc acctaattga gccacagtta
     3661 caccacttgg aatatcccat gctggaactg tattaatcat cgcactaggc aatatttcct
     3721 ttatttcaga gaagaatttg tgacaatcac tcccgacgaa ataaatagct tcattcagct
     3781 tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt acagtttcta
     3841 atctattttc tttccagcgg tatgccccag cgtatacatt ttttcttcga gcatcaaaca
     3901 tgggcacaat aatattatcc acaccgatgc aattagccgc gatggttttc agactagata
     3961 ctcctactag ctctttatct aatgtataag caatcgtttt tgctgtcgtt acacctatac
     4021 gaagccctgt ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg tcttttggac
     4081 ttatttgaag atctgcaaac aattggtcga ttgcaggcat cagtgttaca ctgtggttgc
     4141 gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca
     4201 aagtttggtt tgcagtatct attcctaatg taatcatata tttgatcctt tctgctatga
     4261 cattctcgcc ttccattgta gcatactttt ttattctgag gatgaaaata ccaaaggcaa
     4321 aactagtaca ataatacatg attatctgct ggcgggcaaa ttttgcccct tcctgaaaca
     4381 caaaaaagct gcggtatcac tccattttcc cacagctttt ccatctttta tttcgttgat
     4441 tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc tttggaatta
     4501 cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata aacttttaca
     4561 gtagaaagcg agggcgttac atatttcgct acactgacat cattgaagcc catcacagaa
     4621 atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc aatcgctaat
     4681 gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa atattgcttc
     4741 actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc ttcgtcgtag
     4801 cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag ccgctgatgg
     4861 tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt atgggagaca
     4921 aagtgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga gtcgattcct
     4981 tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt catttctaat
     5041 aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgccggaag atcactaatg
     5101 gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt ttcgattcct
     5161 aggcgaatag ataaataata aagatcttca agctcttccg tatcgtcgta ccactggatc
     5221 aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata atttaagcgt
     5281 tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc aggatcatag
     5341 ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat gtctttaatt
     5401 gttgccattt gtctctcctc cttttttacg tttataaaat cgcagtaaat cgttcaaacg
     5461 ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa
     5521 cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt
     5581 ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt tcttctccta
     5641 ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa acagcaagcc
     5701 ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct ttatgtggat
     5761 gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca aggtccaact
     5821 caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg
     5881 ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccatttt tccaatactg
     5941 agttagctgc ttcaataagc aagtttttct ctgttgattg caaacggatc acagacatcg
     6001 gccatttcac gactcctgcg atgacttgcg gataatcttc tagtttgata agtttttcta
     6061 tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca tgagaaagaa
     6121 tcgatcctcc aacaattggt aagtcagcat tcgaaccaac aaagtaatga ggcaagactt
     6181 cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt
     6241 ctgataaaat gatcgaatgt tcattgtaat aagcataagg ggaatattgg aatccccagc
     6301 tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga tagttcaagc
     6361 gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct acttttaccg
     6421 cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa ttgattgtga
     6481 tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg atggctcttg
     6541 ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct tcctctgggc
     6601 ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt gtgagaaaat
     6661 ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga acaccatttt
     6721 ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta actggtctta
     6781 cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc tgtaaatata
     6841 agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg
     6901 tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat ggctgacatg
     6961 ccaatcccat gctgttttga taatcgcttt gatatctgtg tattcaggtt tccagcccag
     7021 aatctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag ctcttcttgg
     7081 tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta gcatttcctt
     7141 gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc cttctttcaa
     7201 gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat aatctctgat
     7261 acatgttcca tctggtgtat cataatcgtc tccataaaca gccaaggctt ttctttgacc
     7321 aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat cttctccgat
     7381 agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat agcgcatgcc
     7441 atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt caccatacgg
     7501 atttttagga ttagctggtg tatcttctgt gattggagac tctttcggct ctccataagt
     7561 tgctgcagta gaagagaata cgattttatt cacgttgaac tcgtgcatga cttcaagtaa
     7621 gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga cagattctcc
     7681 tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt caaatacgga
     7741 acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc
     7801 ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct tttgaactag
     7861 ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa ttgtcataaa
     7921 aaacactcct ttgattagta aaattgtgtt tgttgattct atataattta aaaaacgaag
     7981 tttactttta tggtgaaaac aaacaaagta aaatttc
//
LOCUS       DACDID010000117         7964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDID010000117 DACDID010000000
VERSION     DACDID010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.118"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IV236_002098"
     CDS             complement(21..995)
                     /locus_tag="IV236_002098"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8772274.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IV236_002099"
     CDS             complement(1075..1950)
                     /locus_tag="IV236_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8772275.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IV236_002100"
     CDS             2080..3987
                     /locus_tag="IV236_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8772276.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IV236_002101"
     CDS             complement(4528..7806)
                     /locus_tag="IV236_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8772277.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2269 a         1667 c         1472 g         2556 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaa
//
LOCUS       DACDID010000118         7958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDID010000118 DACDID010000000
VERSION     DACDID010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.119"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(182..964)
                     /locus_tag="IV236_002102"
     CDS             complement(182..964)
                     /locus_tag="IV236_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772278.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1029..1865)
                     /locus_tag="IV236_002103"
     CDS             complement(1029..1865)
                     /locus_tag="IV236_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772279.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1883..3292)
                     /locus_tag="IV236_002104"
     CDS             complement(1883..3292)
                     /locus_tag="IV236_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8772280.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3306..5129)
                     /locus_tag="IV236_002105"
     CDS             complement(3306..5129)
                     /locus_tag="IV236_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772281.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5972..6298)
                     /locus_tag="IV236_002106"
     CDS             complement(5972..6298)
                     /locus_tag="IV236_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772282.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6344..6706)
                     /locus_tag="IV236_002107"
     CDS             complement(6344..6706)
                     /locus_tag="IV236_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8772283.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6743..6958)
                     /locus_tag="IV236_002108"
     CDS             complement(6743..6958)
                     /locus_tag="IV236_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772284.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7085..7420
                     /locus_tag="IV236_002109"
     CDS             7085..7420
                     /locus_tag="IV236_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772285.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7496..>7958)
                     /locus_tag="IV236_002110"
     CDS             complement(7496..>7958)
                     /locus_tag="IV236_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772286.1"
                     /translation="FNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLM
                     TEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSF
                     LIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2429 a         1701 c         1244 g         2584 t
ORIGIN      
        1 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
       61 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
      121 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
      181 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
      241 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct ttaccaactt
      301 gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
      361 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct
      421 tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg
      481 tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg
      541 tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac
      601 ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt
      661 cactataacc agttctttca atagagactt caatgtattt cacactatca tactcaaaag
      721 aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta
      781 cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct
      841 tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac
      901 actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt
      961 ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt
     1021 ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt
     1081 gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct
     1141 ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc
     1201 ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc
     1261 gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt
     1321 agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca
     1381 atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg
     1441 atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca
     1501 atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag
     1561 tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca
     1621 taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta
     1681 atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg
     1741 tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt
     1801 acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact
     1861 ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc
     1921 aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa
     1981 catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg
     2041 accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa
     2101 tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta
     2161 tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca
     2221 atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat
     2281 ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat
     2341 cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg
     2401 aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg
     2461 cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt
     2521 ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg
     2581 caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac
     2641 cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata
     2701 aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca
     2761 aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt
     2821 tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag
     2881 ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca
     2941 tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag
     3001 aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg
     3061 aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat
     3121 ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa
     3181 taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg
     3241 aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc
     3301 tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta
     3361 attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg
     3421 tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct
     3481 gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg
     3541 atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata
     3601 gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg
     3661 ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg
     3721 gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca
     3781 tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt
     3841 acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag
     3901 gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa
     3961 ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt caaggttact
     4021 ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc
     4081 tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca
     4141 atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc
     4201 atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa
     4261 ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt
     4321 gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc
     4381 ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga
     4441 tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat
     4501 cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa
     4561 aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg
     4621 ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct
     4681 gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc
     4741 aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc
     4801 ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct
     4861 gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt
     4921 tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca
     4981 ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc
     5041 agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat
     5101 acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac
     5161 caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat
     5221 aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag
     5281 cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac
     5341 agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct
     5401 aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt
     5461 gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa
     5521 aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca
     5581 atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg
     5641 agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta actagcaaat
     5701 gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca
     5761 ataaaaatac caaacatatc cataataatt ttttcttttt catttctttt atattgttat
     5821 aacagtgaat aacataaaaa ttcccgacaa tcattgtaat atttttttgt cacctgtatt
     5881 agtatttaat ttataggaaa ctataaaaac tccctagtga atttacacct ttgggagttc
     5941 ctgtttggac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagcatatg
     6001 gtatctccac atatatacgg tctttctttg gatagtactc aatatttatg ttgatagcac
     6061 ttccattgtc taatatagct ttagcccggt atgtgttatc tccattttca tgagtaatcg
     6121 tcacatcatc acatgatcga tttaagttgt actgttcttg taaaatctgt gttaccaatg
     6181 gttttgatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaata atacctataa
     6241 tagccaagac aacaatacct ataatgacaa acttcttgtt catatttact ttattcaact
     6301 ttttatctcc tatctccaaa ataatttgct cttatccctc ttatcagtac attgacatcc
     6361 ctaaaagtac accaaagatt gctccagcta cccctgctat catcaaaatc gtacccattt
     6421 tctcatcttt ttctcgatat aagtagccca taataacagc aacagcacca aatacaacag
     6481 gaataaatgc taacgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     6541 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     6601 ttactgtgtc tgttccggtt gtctcatctg tttcttgtag tgctccacaa aactggcaat
     6661 acttactttc atctgttatt tctttgccac attttctaca atacatgtct tctcctccta
     6721 aaatcattca attgcttatt agttaactgt ttactttatt aaatacatca gaaaacagct
     6781 cattcattgg tacacttaat atctcagaaa gcatcttcat ttgatagggt ttaggaatcg
     6841 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt tttgctaact
     6901 ccgtttgaga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     6961 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa
     7021 aaaacttcaa ataccattac aaatacaaca acttgttgta ctatactgag atgggggtgt
     7081 attaatgaaa cagaaacaat atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg
     7141 gacacaaaaa gaattagctg aaaaaatctt ctctacacaa caggcagtag caagatggga
     7201 aaattctgta acagagccta acctcgatag cttaacagct ctttctagag ccctaggaac
     7261 ccctgtaagc cattttctag acaatgttgt cgtagattat gaggaagagt ttttggctct
     7321 ctatcgttct cttagcactg aagatgcagt tcgaacaatt gactatatga agctattaaa
     7381 aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata acccaccgaa
     7441 ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa cctgtctagt
     7501 ttaatacaat tgggtctttt ctcttcctta ctctccgtcc atcatctctt gttacacttg
     7561 taaattgata cagaggaact atcccagctc ctgataatat caattctttt attgtttcat
     7621 ctatccctgc ccctttcgct aaatatatta aaaaactact acttgctttc acattgtata
     7681 gtttcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc
     7741 tgttttttat taaagcattt ttcacgctgt tatccccaac attacgtagg cgttcacaaa
     7801 cagtgttaat aagttctttc tctgtcatta gatatgtccc ttgccacgat aagccctgta
     7861 aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctgagttct gatcgggaat
     7921 tttgaggaaa tactctttta gcctcttttt cgttaaaa
//
LOCUS       DACDID010000119         7947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDID010000119 DACDID010000000
VERSION     DACDID010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.120"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..536
                     /locus_tag="IV236_002111"
     CDS             36..536
                     /locus_tag="IV236_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772287.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(726..1070)
                     /locus_tag="IV236_002112"
     CDS             complement(726..1070)
                     /locus_tag="IV236_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8772288.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1224..2144)
                     /locus_tag="IV236_002113"
     CDS             complement(1224..2144)
                     /locus_tag="IV236_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8772289.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2331..2822
                     /locus_tag="IV236_002114"
     CDS             2331..2822
                     /locus_tag="IV236_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8772290.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2846..5221)
                     /locus_tag="IV236_002115"
     CDS             complement(2846..5221)
                     /locus_tag="IV236_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8772291.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5547..6173
                     /locus_tag="IV236_002116"
     CDS             5547..6173
                     /locus_tag="IV236_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8772292.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6196..6900
                     /locus_tag="IV236_002117"
     CDS             6196..6900
                     /locus_tag="IV236_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8772293.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7092..7925
                     /locus_tag="IV236_002118"
     CDS             7092..7925
                     /locus_tag="IV236_002118"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8772294.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2434 a         1516 c         1439 g         2558 t
ORIGIN      
        1 acaaatgaat aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag
       61 cagatttaaa gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta
      121 tcaacaatgc ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa
      181 aaacgtcttt aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag
      241 aaagtgatga acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat
      301 taagaaacgg cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat
      361 atgccacaca aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg
      421 cacttttgtc tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg
      481 ctgcgattta tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa
      541 gaaggattcc aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa
      601 ttaacttata atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt
      661 ctagaaagga taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa
      721 gatttctatc gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat
      781 gctggatacc agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg
      841 tcatggatag attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc
      901 ttaatgactg tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg
      961 acgatgtcga ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct
     1021 ttgattagtt ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac
     1081 tccttaacac gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta
     1141 gaagtaagat tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt
     1201 tattctaaaa taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat
     1261 acgccagttg ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa
     1321 tgagttggta aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga
     1381 tcatacattt cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag
     1441 ccaccgacag acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt
     1501 acctcttctg gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg
     1561 gcatcaatta cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag
     1621 ttaaagaaga agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg
     1681 actgtttttc cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta
     1741 aagcccattt ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca
     1801 ttcatcaaaa tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca
     1861 catacaacat tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat
     1921 acaaacacag ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac
     1981 cattcatata aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca
     2041 ttggctggaa tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata
     2101 accactattt tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc
     2161 acctctcccc tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa
     2221 tacttcgaac tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat
     2281 gataagatag atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag
     2341 atgctagctt cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc
     2401 aaacaacaaa agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat
     2461 tagagaacag caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag
     2521 atgtattatt tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg
     2581 aatcagaaca cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg
     2641 atcgtgaaaa cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag
     2701 cacaaatcca agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg
     2761 tgattgcttt attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt
     2821 aatcaaaaaa cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg
     2881 ataaccaaaa acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg
     2941 tttgccaatg ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca
     3001 atacaacgat tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt
     3061 tgttcattcc aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat
     3121 atgatttgat cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac
     3181 tgccgatcaa tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc
     3241 aaaatccaat caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt
     3301 cttcctcatt catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga
     3361 atgcttccac cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga
     3421 cctttccatc agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt
     3481 ttcgataagt cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag
     3541 gactcggcag tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga
     3601 gtggtgaaaa actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa
     3661 gaaacaagct tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga
     3721 ctgttgtttc gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa
     3781 aataataagg aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt
     3841 taattttttc gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat
     3901 gatgataatc ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata
     3961 gtttgttgaa acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt
     4021 tcgttttttc ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat
     4081 gtgcttcgtc aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta
     4141 aataaacagt tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac
     4201 tgacatctaa tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga
     4261 cttcacgagt gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat
     4321 tgtaataacc attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc
     4381 agatcttgtc tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg
     4441 ctgtcatagc atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct
     4501 tttctccctc ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc
     4561 ctgttccggt aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag
     4621 ctaccgctag ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca
     4681 aagaggcatt gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt
     4741 cagtcagctt tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg
     4801 tgatcacttc ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat
     4861 gttcttgact atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt
     4921 ctggttctaa atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac
     4981 cgtctctttt gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt
     5041 gaactgttgt ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct
     5101 tgcggtggat tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac
     5161 ttccacgtcg caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca
     5221 tttccaccac ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct
     5281 ctctttccaa acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt
     5341 ggatttttgg atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt
     5401 ccttctcttt agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga
     5461 gaaataagta cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa
     5521 gttttggcta tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg
     5581 aagaaaactg ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag
     5641 atgcggaacg aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc
     5701 ttacccatac tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa
     5761 tcccagttta tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt
     5821 ctggactcgg tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat
     5881 ttgaaatgaa aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc
     5941 attctatagg aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt
     6001 ttagaggaag tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata
     6061 gtattcggac ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg
     6121 atgcaacgac gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact
     6181 taggaggaga ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg
     6241 ttcaatacaa ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa
     6301 gaaggcattc attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct
     6361 agtgacagtg gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga
     6421 aaagaaattc cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa
     6481 ggtggatatg acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat
     6541 gatgaaatgt ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta
     6601 gataccgctg ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt
     6661 gaagatatcg cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat
     6721 ggtttgacga tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg
     6781 cttttaaatg gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc
     6841 atcaatgata cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa
     6901 tggggatatt ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg
     6961 tcttcttttt tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa
     7021 aaaacagatt gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa
     7081 ggaaggaatt tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag
     7141 gaataacgga atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca
     7201 tcaaactgga tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg
     7261 cgatcttggc tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat
     7321 caacggtaga aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac
     7381 cagccatcat cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct
     7441 taccagttgc cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca
     7501 acaaaaacaa aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc
     7561 ttgttgggtt attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga
     7621 tcctaggatc tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct
     7681 tagggattcc tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag
     7741 ggaattcatt caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg
     7801 tggtttccgt cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg
     7861 cttttggctg gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa
     7921 tgtagaaaaa accagaacac ttctctt
//
LOCUS       DACDID010000120         7912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDID010000120 DACDID010000000
VERSION     DACDID010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.121"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..641)
                     /locus_tag="IV236_002119"
     CDS             complement(<1..641)
                     /locus_tag="IV236_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8772295.1"
                     /translation="MQAPIKKICVHFETKQWRNKMVATVTLYLTFTLILDLGIYLFFS
                     KQLLVKNSFGCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFF
                     AITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPT
                     EHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLSIVISFFSLFFSIMLIS"
     gene            642..1382
                     /locus_tag="IV236_002120"
     CDS             642..1382
                     /locus_tag="IV236_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772296.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1468..2226)
                     /locus_tag="IV236_002121"
     CDS             complement(1468..2226)
                     /locus_tag="IV236_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8772297.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2223..3251)
                     /locus_tag="IV236_002122"
                     /pseudo
     CDS             complement(2223..3251)
                     /locus_tag="IV236_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3271..6438)
                     /locus_tag="IV236_002123"
     CDS             complement(3271..6438)
                     /locus_tag="IV236_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772298.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(6662..7546)
                     /locus_tag="IV236_002124"
     CDS             complement(6662..7546)
                     /locus_tag="IV236_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8772299.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(7641..>7912)
                     /locus_tag="IV236_002125"
     CDS             complement(7641..>7912)
                     /locus_tag="IV236_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772300.1"
                     /translation="AFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLL
                     FLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2268 a         1681 c         1372 g         2591 t
ORIGIN      
        1 aaactgatca acataatgga aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga
       61 gatagaagca gataattaga tgagcggaag tgaacacttc ccaataccaa gtaaatcacg
      121 ccccagctta ataaaataca aatacctcca aaatagtgtt cagttggata agtaaaaagc
      181 aagttgagaa atctcgtctg tttgtctata ctttttacta atagatagaa aataaatgcg
      241 ataagtagtg caaaaaaact tgctgttcca agatgtctct ttcctaaata atccatcaga
      301 tcaaaaacca gaaaaaagct gagtccagtg atagcaaaaa aaagattact gatcaaacag
      361 ctgataaaca ggtttttcgt agtaaaataa aaaagaaaca tcaataataa gccaactagg
      421 taaggaaagc tcactttagg gataggaagt aaaagtgtca ccaccatcaa agctatacaa
      481 ccaaagctgt tttttactag cagttgtttc gaaaagaata aatagatacc taaatctaaa
      541 atcaaagtaa aggtcaaata aagtgtcact gttgcgacca tcttattcct ccattgtttt
      601 gtttcaaaat gtacacatat ttttttaata ggagcctgca tatgaaaaag tattttatca
      661 tagaagataa ccagtttcac agcgaatgga tcatcagaga attggtcaat catcatatcg
      721 agaatatcgt gcagctcact tctatctctc aaatcaatca gttgatccag ttgggcacta
      781 ttgataatca cgatgttttc tttatcgata tcgacttgaa gcaatcactc acaggtattg
      841 atgtcgccga acaaatacgt aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt
      901 atacgaattt tgcagtagag atcatcaatc ggaaaatcct tccgattgga tatatcagaa
      961 aagacaggaa tttatctgaa caaattcgtc aagtgctaga acagatcaat tggaaagaac
     1021 agcagatcgt aaatgtcgat aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt
     1081 atcctcggga aattttatat attgaaagta tcaaaggttt gaaaaaccaa gttatggtca
     1141 aaacaactac agaagataaa ttagtgaata cttctttaaa acaattgaag acagagttag
     1201 aaaagtatgc ttacttttta cttttaaaat cttactgtat caacttatcg caattgcaga
     1261 aaattgattc tgctcattat gtgttagaat ttttcaacgg tgactcctta ctcgttggtc
     1321 gaaagatctt tgaaaagaca aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt
     1381 gatgaataag agctgagacg taagcatagc agatttcagg gagtgggcct tgaatcggct
     1441 ccactccctg aaatcgttga tatgatttta cgcttcctct tctgtaggag catctgttaa
     1501 cgaccatgtg aatgttgaaa cgtagttgtc tacagcataa ttggatacag gaacaaataa
     1561 ttccactgat tgtctccctt gttccacatt ctctcctaat gaataaatcc atgtgccaat
     1621 cccttcattt gctcctgcag ttactagatc tactgcctga tctgctggga tttcgattcc
     1681 ttcagcgcca gttccagaaa cggtagatgg cattccactg ctattagttg cgctatttcg
     1741 aatcacggcg tttttgaatt tcacctttga accagttagt ttattcgtat cagatgttgt
     1801 accaataaat tctgttcgtt tgactgataa tttccaaccg gcataatttc cgcttttatc
     1861 cgttacttgt acgaaattct gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg
     1921 taatgcagca tatactgttt ttgctgtacc taacttgatt gttccaaaat caagatctgg
     1981 agcttggtca atcgtcagta atccggatgt tccctgattt ggttcgtcag gtgtaaaagg
     2041 agtatcagga tcatttggat caacaggagg aactggaaca acgtcttcag gatttgcagc
     2101 atctggtttg gtcacagaaa tcgttccttc tgtagtggct gagctcttat tctcagcaaa
     2161 aacactttgt gtacataata atagacttcc agctagaacg gtacttaatg caattgtttt
     2221 tttcatttct ttttccttct ttcttttaaa ataaataatc cagcaaataa gccaataaga
     2281 atgactccaa caagtatcag ccatcgccag tcaaatgttt ctggctgatt ttttaaatca
     2341 atatcctttt cattcaattg gtccgctgca tcatcagtta tttcaaaatc ggtatggaga
     2401 ttccagcgtt tttctttcca agcacccttc acaaccgctt gataatgtcc cggagctaat
     2461 ttttttcctt ctaatgggac aagtaaatca aaatttgtat tgggtgctac atctattttt
     2521 tcttttttta ttgaaaggta aggctctttt gtttcttttt tgtagatttc aaccgatgta
     2581 gtcaagccga acatataaga agcatttgga ttttgaagat tcgccttaat cacatttctt
     2641 ccatcgcttt gatccgcata agcttttttt aatttcaaat ctggggaaga ctctttttcg
     2701 ctatttcaaa tcaagattgc tttcacttga gaaaactgat tgacgacacc aactgtactt
     2761 ttgttagaaa cgttctcttt tttttcttga aacgtgatcc ctcctgcgac cacaccagcc
     2821 agctcttttt ctggcgcttt cagtgaaagt gtctcagtga ccgcactttg aggaggaaca
     2881 gttatttctc ccactgtttt tgctaagttt ttcatatcat aacttaatgt ttcatcaaat
     2941 ttactttttg attctccata ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc
     3001 aatgtctcaa ctgttactga tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt
     3061 gttttcccag gctccatcct tacatttata tatgttttgt tcttatcaat ctgattagcc
     3121 ggtaattcaa aatctaccgc aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg
     3181 atgaatccaa ggagaaggat cataagtaaa gagcagttgt ttcgatgact tttattccat
     3241 attcgattca tcaatcatcc tccttgatct ttattctgtc ggcgcatctg ctaatgtcca
     3301 tgttatctct gctgagtaat taccagctat ctgattttct ttggttaaga caagtttaaa
     3361 cgcccgaccg ttatttaaat attctgaata atctgcttgc acattcgttg aattttgtcc
     3421 tctgacaatt tccagttctt cttcattcac taattgatac tcctctcctt tcttgaaata
     3481 taggacatcc tgcatagaaa atcctgcatc atttgtgaaa ggctgagata attttatcgt
     3541 taagatccac ggatttttcg tttctcgctg atcataaatc cataattttt cctgtatatc
     3601 catttggcca aatgcttctt gtgtcaaacc agttgctttg atctgaccaa aatcgagttt
     3661 ggaaggggca agaaatgaga gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg
     3721 cggggcatca tgataaacaa ctggcgtatc acttggattg tgattcccaa tttcatcgtt
     3781 ggtacttggc ttgttctgca cacccttagc agtagcttct cctgcttggt cgtttaatac
     3841 aatttctaat acatctcctg caaaaagttg attttctggg atagaaagaa caaattcccc
     3901 tgtatcatca atagttgttt ctgacaaaac agcttgattt tttttcaatg aaaccaaggc
     3961 tcctgcttca cctgtacctg tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa
     4021 attagcaggt tcaggaggaa tcacatcgac tgtcgttttg gcagagtcca gttctgtcac
     4081 ttcttggcca cctcttactt ttttagcagc aacaacttgc acttgatcct ccgtttttaa
     4141 tctctcatca ttgggtactt gaacttcgaa caagccgcct tgttcgcctt ctccccatgg
     4201 gttatatcct gccccttcat tttcatctgt ttgtccttgc gtgagagcag taatttctac
     4261 agatgatttc gtcgtatctg cgtaagtaat acgtaaagtc acttctactt cgcctgtcca
     4321 agcatctctt ggctttgcct cgacaccttc gggaacaacg acatgcccat agattttttt
     4381 atctgcattc gttggcactc ttaaggattc aatcatcggg agctgattat tggcactgat
     4441 tcgattataa tttttcattc cggcacctgt ctgagcaaat tcatttgtta aacctgcact
     4501 agggcttgat acgaaattgc ctaagtcggt cccattgatc gataagtctg tcatatcaga
     4561 aaaataatcc gggtcttttt caaagttatc gaatgttgcg cctgtttttc gccataaagc
     4621 gatttctgaa tgggcgatct ttaaagaaga atctccattt ccttggaata cccagccgcc
     4681 tatgccattg gtctcgcttc caatccgttt gttttggaaa tcaaaatagg taggagattc
     4741 taagtccaca ttcaacacat ccccactgat gatgccggtc ttatcttccc ctgttttccc
     4801 gaccatgact aaataagttt tttcacctgc cgtaaaattt aaatcaatat ctccagttgt
     4861 atctacagtt gctccggaat ctgcgcgtat atcgatattt gatttttctc cagtgactgt
     4921 aaaatcagct gaacctggac tttctagaaa accagaagcg tcataaaatt ggatcgcttg
     4981 atttccttga ttgagtctgt tcacgtaaga tttgccatca cctgtatttc ttaccctcaa
     5041 tgagcctcca ttggttactg tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga
     5101 acgcccactc tgcttatcga tgtcgataac tgatccggct ccttctacga tcaaagaaag
     5161 gccttttcct acaaaacgca tcccattgtt cccgggttga tcgtcatctc cagccatttt
     5221 gaccgagatt tgtccaccac ttgatacatg gatttgacta gaatctcctt gcaagcgaaa
     5281 tccgcccgta gttgctgtat tgatggtgat tttcccacca ttcgatacag tgactttcga
     5341 tcctgaacct ctcatttgga tgattccgcg attgttgtca ttggtaatat tgatctttgt
     5401 atttaccgaa aattcgcttc ccgaaccact gacttcaaac atgacattac caggtagcgt
     5461 atcgactgcc tctccttcat cgctaacgcc atctgcccga tcataatcaa gagagacctt
     5521 tgaaccgctt aagatatgga ttcctggcgc tgtttttcca ctaccagtct catctcctga
     5581 atctagaaaa ccgtagaatt tccctagata cggtccataa agttgactac cattgatcac
     5641 attcaattga tagcttctac aaaaataggc tttacccgga gcggcattca gtccttcctt
     5701 cactggttgg acatccaaca caccggaaac accatctaat gtgacagttg cacgaggagc
     5761 gtaaatcagt cctaatttgg acccatcata caaatcaagt gtcccggtaa attttatttc
     5821 tcccgtaaaa tgatatctgc ttccccaaaa atcggtcata taagcaatga taatggcatt
     5881 tccatcgata tttggtgtca atccacctgg atgtgcagct gttcctttaa aattctggaa
     5941 ctcgatcgac tgaggtatct cattgatata aatatggtgt gtgccgaaat tgtgagcgaa
     6001 tccaccaaaa tcaataacga tatctcgttt gatctctaat tccctgtcta aagtcaaagg
     6061 ctgaatcagt cggatcttag ttactgattc gtctgcgaga agctgcgcca gttcttctgt
     6121 tgtggcagct tcgctgtacc catccgctga aacagctctt gccagtgccg cttcactgct
     6181 atcagacgtt tgttcagcta cttctgaggt ctcacttgtc ttattttctt cttgcctttt
     6241 attagtagaa gcctcacttt gagattctgt agtttcagaa gtaatcgatt cttctgaaaa
     6301 agtagtcttg tcaaaacttt gattctctga ttgcgttgat tcgtttgttt tctctacatc
     6361 ggcaaaaacg gatgtcacca tcaagagagg gaagacaaac aaaatcgacg caatcagctt
     6421 ttttacacat tgattcattc tccaccatcc tctctgacgt tttgacaatt ttattttatc
     6481 gttttaatct atttccaatt gtactttcat atttaaaata tgtattttta tggaaaaatt
     6541 tttctattag gtgtaaattt cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc
     6601 acagtccctt atcccgaaca agcggtgttc tcccgccaac tcctcggtca caacctccat
     6661 tttaacaata aacctgtaaa tgtgagggca atactttgat tgtcacaggt aatttatccc
     6721 cttcatctcc atcaacattc gtcgtcaatt cttcctcatc agctaatgac acaactaatt
     6781 gctcacaagt aaggtaggct agatttttcg tagattcatc tactcctttt aataggtctg
     6841 gcaatgtttt aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa tggagcttac
     6901 catcatccac ttttgcattt ggaagtaatg tttcaaaacc gccgactgag ttagttagtc
     6961 ctatcagcaa ggtactgctc tcaatttctt ccttctttcc atcaacttct acgtgaaacg
     7021 agtaggattg tgtggaagct aactgtttga ttcctgacat gaaataagct agttttccaa
     7081 atttcgtctt cttctccgca tctacatcat tgattgattc agggatagag ccaattgcga
     7141 cgacattcat aaaatagtcg tcatttattt ttccaatatc caatggcttg actgattcaa
     7201 tcgaaaaatg attgatcgct tcctcaggtt ctagcggtat ccctaatgct cgagccaagt
     7261 cattcaccgt acccaatggg aaaaatccga aatttggccg atgttcttgt tcagcgattc
     7321 cactgattcc ttcgttgact gttccatctc cgcccatgac aaacacacta tgataccctt
     7381 ccgtagccgc ttcgcgagta aagttttttg catcccctgc tttttttgta tgcaagacga
     7441 ccacttcatc aaacaccgat tccaattttg ctatcgctaa ttgctcaaat tcctttgctt
     7501 gttcaccgcc tgaactgggg tttaccacta ataaaacttt tttcatcact ttctttccct
     7561 tctgattgat ttctttaata taacgatagc ataaagaaaa aaattaggct gtgatgaagt
     7621 actgttcatg aaacattcat ttattttttg acaagataaa acatccatac tatgaaactg
     7681 aggaaaatta aaacaatgat ataggaaata aaagaaaatg ataggcattg tatacctaat
     7741 aaagtaaaaa tcttaggtgg cagataaaaa cacaaaaata gcaagagaat gtaatagcgt
     7801 ttcttccact ttttgttatc agaccttata gcgatcagag gaataccaaa tgcataactg
     7861 aaaaactctt gatgtactgt taatatgtca cggccttgaa taataaatgc ga
//
LOCUS       DACDID010000121         7850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDID010000121 DACDID010000000
VERSION     DACDID010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.122"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            149..1915
                     /locus_tag="IV236_002126"
     CDS             149..1915
                     /locus_tag="IV236_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8772301.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2017..3276
                     /locus_tag="IV236_002127"
     CDS             2017..3276
                     /locus_tag="IV236_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8772302.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3423..4724
                     /locus_tag="IV236_002128"
     CDS             3423..4724
                     /locus_tag="IV236_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8772303.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4727..5578
                     /locus_tag="IV236_002129"
     CDS             4727..5578
                     /locus_tag="IV236_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8772304.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5591..6406
                     /locus_tag="IV236_002130"
     CDS             5591..6406
                     /locus_tag="IV236_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8772305.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6557..7723
                     /locus_tag="IV236_002131"
     CDS             6557..7723
                     /locus_tag="IV236_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8772306.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2548 a         1359 c         1571 g         2372 t
ORIGIN      
        1 aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact
       61 gttaccgata acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac
      121 gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga
      181 aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa
      241 aaatgatatc aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa
      301 atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg
      361 ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga
      421 agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat
      481 tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat
      541 tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg
      601 ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga
      661 agactttttc gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct
      721 tggcatcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga
      781 tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt
      841 gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat
      901 gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa
      961 agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga
     1021 aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa
     1081 tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga
     1141 attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa
     1201 aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg
     1261 gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt
     1321 tacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag
     1381 tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt
     1441 attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat
     1501 gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac
     1561 agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa
     1621 ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca
     1681 gttgtctaag attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga
     1741 taagctctat ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc
     1801 aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt
     1861 gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact
     1921 gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg
     1981 tgtaacagca cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag
     2041 ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt
     2101 aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct
     2161 ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa
     2221 atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct
     2281 gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag
     2341 caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa
     2401 gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt
     2461 ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat
     2521 ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta
     2581 gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta
     2641 tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa
     2701 ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata
     2761 aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt
     2821 gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa
     2881 attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta
     2941 gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat
     3001 caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct
     3061 agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa
     3121 acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac
     3181 ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta
     3241 aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt
     3301 ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt
     3361 gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc
     3421 agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta
     3481 ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca
     3541 tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg
     3601 ttactttggg aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt
     3661 tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt
     3721 atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca
     3781 tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca
     3841 tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg
     3901 acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta
     3961 gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga
     4021 tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg
     4081 ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg
     4141 cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg
     4201 ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa
     4261 tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt
     4321 taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact
     4381 tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc
     4441 ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca
     4501 ataatttctc catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag
     4561 ctggttctac tgatattctt atttcctgga tctataaact tacgactgga acttctccgc
     4621 agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat
     4681 tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca
     4741 ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat
     4801 actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg
     4861 aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg
     4921 gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct
     4981 aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg
     5041 ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg taccgactat
     5101 ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg
     5161 gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg
     5221 atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca
     5281 tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc
     5341 attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac
     5401 acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca
     5461 aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt
     5521 cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga
     5581 gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca
     5641 agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt
     5701 agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc
     5761 attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc
     5821 tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc
     5881 tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat
     5941 tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta
     6001 tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca
     6061 acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag
     6121 tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt
     6181 tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc
     6241 aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat
     6301 ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt
     6361 atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat
     6421 ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa
     6481 aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt
     6541 tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt
     6601 ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga
     6661 cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag
     6721 taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag
     6781 tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga
     6841 ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat
     6901 ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac
     6961 ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa
     7021 agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg
     7081 ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg
     7141 gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt
     7201 agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga
     7261 tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt
     7321 caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg
     7381 acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt
     7441 ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt
     7501 ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca
     7561 agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata
     7621 tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct
     7681 tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg
     7741 tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt
     7801 ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
//
LOCUS       DACDID010000122         7689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDID010000122 DACDID010000000
VERSION     DACDID010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.123"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            124..2451
                     /locus_tag="IV236_002132"
     CDS             124..2451
                     /locus_tag="IV236_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8772307.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2577..2906
                     /locus_tag="IV236_002133"
     CDS             2577..2906
                     /locus_tag="IV236_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8772308.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2946..3950
                     /locus_tag="IV236_002134"
     CDS             2946..3950
                     /locus_tag="IV236_002134"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8772309.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3947..4393
                     /locus_tag="IV236_002135"
     CDS             3947..4393
                     /locus_tag="IV236_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8772310.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4543..5358
                     /locus_tag="IV236_002136"
     CDS             4543..5358
                     /locus_tag="IV236_002136"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8772311.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5564..6736
                     /locus_tag="IV236_002137"
     CDS             5564..6736
                     /locus_tag="IV236_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8772312.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6898..7392)
                     /locus_tag="IV236_002138"
     CDS             complement(6898..7392)
                     /locus_tag="IV236_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772313.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2609 a         1453 c         1522 g         2105 t
ORIGIN      
        1 aaataacctt agaataaaaa gagaaaaaaa gccccaaact ttctatacat caataaaata
       61 ataaaaatta aatttagaat aacgtgtaaa ggattatttg atgatttttt attgagagga
      121 gggatgaaaa tgattaagaa aactcaaaat aaaatccaac gtatatgtca aaaacatttt
      181 gtttctagac tcaaagctgt atctaaaaga aaatacttac atcccattat gatttcgatc
      241 atcttgattt ttactttctt agggagctta tcaagtattt atgtaaacgt ttatacagaa
      301 acgtcaaatt ttgctaacca aacgacagag gagacaaata gtccacccta ttctgaaata
      361 cagtccatag aaagcaccaa tcagtctagc aacaacgaga caacctcaac cacatcctcg
      421 actcaactga ttgatccgac agaaaaagaa atcacagcag caaaagcagc aggggagaaa
      481 gcttattcag aaacaggtag aagccagaca tttagtactg ttgcggccac tgcgaatttc
      541 aacggacata ccataccaga tggatataca gtgatatatg ccaaagatgg agtcattgtt
      601 gcggttaacg gatcgatgag tgcggataaa actgcgttac aaggcgtaac caaagcaatc
      661 gcttttattg ataaccgtcc agatatccat aatctgttta ttggctccac agtaaatggt
      721 actactatca cattcccaaa aagtatctat atgggggtgg ctaccgctgg aaatacgatc
      781 gattcagtta cacccaatgc ggataaaagt tattctttta caccctcaac atggggaggg
      841 cttcctgccg gaggaggtat gagtactttt tcaatcggta tacagttagc aaatgatatt
      901 ccattcacgg ggtactcgat caaaaatggg cagatcgttg atggtaaggg aaatgtttca
      961 aattttccaa cctatcaagc agcaacggtt caggtcaatg cgaatcggac aggaactttt
     1021 tcttatacag tcaatggtgg agcagcaaaa atcggaacgt acgggactac attatctgga
     1081 atccatgtgg gtgacaaagt ggttatcact ccaacagcag taacgggcta ttcttttgca
     1141 caaacaaacg gttctttcac agccctagaa agtaacgtcg atacggtgac ctatacaggc
     1201 aaacaaacac caatcactat taagtatcaa gactattttg gacagatgat tgctccttct
     1261 aaaataatga atcttactta cggaagtgct gcacaagacc tgacgactaa tacgcctact
     1321 atcaacggat atacctttac agctgtttca gcgatagaaa caaagaacca atcagcaatg
     1381 agtgttttag ctagtttaga tacaaatgga aatgtaatcg taaaggacgc aaatggaaaa
     1441 cagatttctg aagtcatcct atattataag acaaatgcaa cagtatcaat caatgcaaat
     1501 ggcagtaagt actatgatgg tctttctgtt gtaccgatcg ttaaatatgc ctttaatgat
     1561 aaaacagaat caacatccct tttaaacaat gatctcagta ttgatcaaat cacatggaat
     1621 ccaactgatt tcaaagcgat gaatgaaaag ggagttgaag taactgcgcc tactgaaatt
     1681 ggaacctata cgtcttggca attgacaaaa agcggactag ctaaactagc tgccagaaca
     1741 aactatttat ttactatcgt gcagacatct gatgtctata cgattaagca aatcagcggt
     1801 gcggtagagc tcagtggcag taagacatat gatggaaaag caggtgtccc gtcaatccat
     1861 gtcaagctag cagagggtgt cactagttcg cttactccag tacaagtggc acttagcagt
     1921 atggattaca cagcagatgc acaatcagca aaaaatatgg ttaatgctgg cagctataca
     1981 attaaactaa cgaatagcgg aatagacaaa gtcaaagcag caaatcccaa gttgtcattt
     2041 actgatatag ccaacacatc aggaacttat accatctata aagctaacgc agtgattact
     2101 gtcgatgacg ctgcctttaa ttatgatgcc caaagtcact cgatttcggt tggaaatctc
     2161 cacgttaccg gtgttgtctt aggcgaatca ttggattata ccctaacagt taacagccgg
     2221 acagatattg gatcacaaat agtaacgatt agtttaggcg aaggaagtgt aaacaataat
     2281 tatacgatta tcgcaacaga taccgctaag ttgacgatca atccgactct cttgcttcct
     2341 tcgacaggtg ggataggtct actgcccttt gtctttttag gactgatctt tattttttct
     2401 ggcttctttt attttatcca tcgtaagaaa aaagcaggtg aacagcgatg aaaaaaacaa
     2461 aaaatgtacg tgtggatata tttgctgttc tcttttttat cctactggtc ttttttatag
     2521 gttctccgac tgtgtcagct agagaatcac ctattacatt tactttgcat aatcgaatgt
     2581 tcgaaagcga taagatgcca agtatcagtc aaaacgacgg aataaatatc ccggataact
     2641 ttcaaggagt aaaagggttg aacggcgtta ctttcgctat atatgatgtc tcggatgaat
     2701 tttacaagct gcggtcagaa ggaagtagtg tggaagacgc acaaagaaaa ctagcacaaa
     2761 aaagtgatag tgaaaaaata ctggcagaaa atgtgacaaa aacagtagac gaggaagagg
     2821 gcattgcttc ttttagtgca tcggacaaag atgagcaagg tcgtgacgct gtttaccgat
     2881 ttacggaaat aaagacttca gattaagtaa aagaaaaatc agcaccattt gtcgttgtgc
     2941 ttcctgtgac agattcatct aatcataaat tgacgaccat ccacttgtat ccaaagagcg
     3001 aacaaaaaat acctgctgca ctcacattaa caaaaacagt agagaataag cagacagatt
     3061 ttgcagatgg ggataaagtc ccttatctca tcaccgtaac aattcctgag aacatcaacg
     3121 agataggaac ctatacgatc aaagatacag ctgatcctca gttatggcta gaaccagaaa
     3181 cgatcgattt tttgattggt ggagacaaga ttcatacatt tcacacacaa aaaataaaac
     3241 atggttttgt cttatcagat tctggaaaag atcttagttc gtttgctgga aaaaaattaa
     3301 cgatccgtta ccaaatgaca ttgaaagaaa acagtgaaaa aacgacattt gataacgatg
     3361 tacgtttgac gactgacaat caaacagtag aaacacatct tgccatccaa acaggaggca
     3421 aacaatttgt caaagtggat cggcaaacga aacaaaaact agcagatgct caatttctag
     3481 tcaaaaaaaa tgaccaatac ttagcgcaag aaaacgggat aaatcattgg gtagccaaag
     3541 atgattcaag agcaacgatt tttacttcca gtaaagatgg aacattttct gtccatggat
     3601 tgtcttttgg aagttacgaa ctagtggaga ttaaaccgcc taaaggatat atattgagtc
     3661 aatcaaccat tctctttgaa gttgaaaaag gcagttatga tccgcttcat cctataccat
     3721 tggaaatcat caacgaacca aaagcaaatc cgacgaataa tggaaaaacg ccagttcaga
     3781 tgcagacaaa tggtccatca aatgaaaaaa atggaggaag ctttccaaaa acaagcgaag
     3841 agatgcttgg aggtttctca attttagggt tattgctagt cctttcaaca gggacagctt
     3901 ggttttataa aaaacaacaa aaaggaaaca ataggaggga aataaaatga aaaacatcaa
     3961 aacaaaaata tggacgtttt taggaactgc tatcatgctg cttcctttcg ttctcggcct
     4021 aggaactgca gaggtttctg cagccgtatc acctacacca gaaaatgtta cggttaattt
     4081 gcataaattg aaattcactt cagcaccaga aaatcaaata aacaacggaa cggaattgac
     4141 atttccgaat agcgaaccgt tgaacggcgt tgaattcaac gtatatgaca tcactgctac
     4201 gtactatcca agtaaagaca cagcagttcc agcagacgct acacccttcg caagtgtaac
     4261 gacttctggt gaaggattag ctaacctgac tcttccaggt aaatctgatg ggaaagatgc
     4321 tgtatacgtc tttgtagaaa caccaaagcc cggtgtcgaa acgtcgccaa atatcgtgtt
     4381 aagcttaccc tagaccctga caaaccttta gacacgattc acctatatgc taaaaatgtc
     4441 atcaaagaaa gcaatatcaa aatccagaaa gctgggaact tttcaggggt agataagtta
     4501 gcaggggcgc aattccagat caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa
     4561 aacactggtg tcgctctcta tgatcctgct gagtcagcgt tgacattcac aactgatact
     4621 gaaggatcca tcagtgtcaa tggattaata gacggcaatt atactttagt tgaaacaaaa
     4681 gcaccagatg gttatcaatt aaccaatcca caaaaagaaa ctacttttat agtaaaaaac
     4741 ggaaaattgc aacaaacaac cggtatcatc catggcgcag atgaagccaa tcttgggaat
     4801 ccaaacggtg tagtcaatcg ggaaaataca actacggata acacgattac tgtagaaaat
     4861 gtccaaaact acggtgattt ccacttcatc aaacaagatg tgaacacgca agaaaaatta
     4921 gccgatgcag aattttctat tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa
     4981 atagcaaacc ttgcctcagg agaataccca tatgcttggt cagatgaagt cacaggttct
     5041 gactgggaag aagtgaaatt gatttctgat tcagaaggga aattttcaat cgaagggctg
     5101 aaacaagggg catactgcct tcaagaaaca aaagcaccag aaggctacgc attacctaag
     5161 aatgaagctg cgtacactga atttacagta gatgatgatc caacaacttc tgatgaaaca
     5221 acgatcgata accaacctaa aggaatcttg ccttcaactg gtggtatggg gatcatcgta
     5281 ttcatcttgg taggtactgc tttagttgga ggagcagtga tctatttcaa aaaacgccat
     5341 caagaaacag aagcttaaac tattggaaac aaaaatcata cgataaataa acgatagtga
     5401 ttcaaatggg gagaaaggtt gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg
     5461 gcaaaatagg aggaactatt ttgccgtttg cttcgctaat actcaggtca aggcgctgat
     5521 ttcacaacct cttcatatac atattagaaa ataggtggac atgatgaata aaagcagaat
     5581 aagaatactg gacatcttca tggcaatcat tttagtcgtt gggatcggga tcttttctta
     5641 tccttttgta gaagattcac tgaatgattt tcttgcccag caaatgatca tccattatca
     5701 aaagcaagca tcgaataaga attcagagga aataaaaaaa cagcaggaaa aaatgacaaa
     5761 gaaaaaccaa cagttggcag agcaaaacgt atcaccaggg ataggcagtt tcaaccaagc
     5821 agttgatgca aaagcattaa aagatttacc tagtaatgcc ttttttatgg aacatatgct
     5881 tggagtcatc gaaatcccaa aaatcaatgt cagcttacct atattcgatc agacaacgga
     5941 aatatttttg caaaaaggaa cgtcattatt ggaaggaagt tcttatccga ctggcgggaa
     6001 aaacacccat gccgttcttt ctgggcatcg tggactccca gaagcaaaat tatttacaga
     6061 tttgcctaag ttgaaaaaag gagaccagtt tttcatccag atcaatggaa agacattggc
     6121 gtatcaagta gaaaaaatcc aagtggtctt acctgacgaa gtagattctt tgggtatcca
     6181 aaaaggcaga gatctcgtga ctttgcttac ctgcactcct tatatggtca atacacatcg
     6241 actgcttgtg acggggcatc gtattcctta tcaagcaaaa gaggcaaaaa aagcgatcca
     6301 aggaattgat caatggaaaa aatggaaatt ttttatttgg tttatcggta tactacttgg
     6361 atcaatcggc ttagtttggc tactcatcgc ttatttagat tctttggcca tcgctaagcg
     6421 aaactatcct ttatcttttt atgtaaagaa cacaaacgga cgaccaatcg aaggaatggt
     6481 cttttctgta aaaacgctca acggcaaaca ctatattact agagaaaaag ttccatttgt
     6541 gaaagcttct gatgaatacg gactagtgag attttctgat ttaaaaggca ggaactatcg
     6601 cttgcaacat gaggagctcc tgcttaaaat ccatgtaaaa cataaacatt cgaaacagtt
     6661 ttcaatgaaa ttgaaaaaag gaagatataa actgcgaaaa gaaaaagagg tgtactattt
     6721 gattgagaaa gaatgaatga agatgtccaa aaaagcagag ggaacagact aaatgaaaaa
     6781 agagagaagc tgacgaaatg gacgtgtaaa ctgctatttt tggtttacta tctggaacgc
     6841 cagcttctat ttttttatgt ttcagaagaa ttttattatt atagtataac ttttgaatta
     6901 gtttccataa catttggagc aaggggttaa accacgagct tgagcttcgc ttaaagtggc
     6961 aggggtatac gtaccatttc cacatttatg tgtatggtat tttgaaccgg tcggtgtgac
     7021 atagacagtt tgttcattat tttgctcagg agctgcggct tgtgcctgct gagaagcctg
     7081 ttcagctgct gcttgctgtc gcgcctgttc tgcttgttgc tgtgcgattg cttcttgcct
     7141 cgcctgttct gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg cttgttgtcg
     7201 tgcaatttct tcttgctttt tccgttcttc ttctgcttct tttgctttga ttgtggaatc
     7261 aattgcagca agacggcttg tcaactcttg tttgttttca ggtagcgctt ggatagctgc
     7321 tgaagcagtt gcatagtttt cttttgtagg atttgcttca gcttgtgaca aagcagattc
     7381 cgcttctgtc actttttttt ctagtgcagc tttttccgct ttttgtttct cttcttcttt
     7441 tttcttttca atttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct
     7501 gctggaagcc tgagctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact
     7561 caacaaactt cctgcgacca aaaacaacgc aactaatttt ttcactaaat ttcctcctta
     7621 agtaaaaaac aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat
     7681 aattgtgtt
//
LOCUS       DACDID010000123         7584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDID010000123 DACDID010000000
VERSION     DACDID010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.125"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(57..785)
                     /locus_tag="IV236_002139"
     CDS             complement(57..785)
                     /locus_tag="IV236_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772314.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(798..2078)
                     /locus_tag="IV236_002140"
     CDS             complement(798..2078)
                     /locus_tag="IV236_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8772315.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2219..2821)
                     /locus_tag="IV236_002141"
     CDS             complement(2219..2821)
                     /locus_tag="IV236_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8772316.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3066..3272
                     /locus_tag="IV236_002142"
     CDS             3066..3272
                     /locus_tag="IV236_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772317.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3584..4303
                     /locus_tag="IV236_002143"
     CDS             3584..4303
                     /locus_tag="IV236_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8772318.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4384..5067)
                     /locus_tag="IV236_002144"
     CDS             complement(4384..5067)
                     /locus_tag="IV236_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8772319.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5285..7471
                     /locus_tag="IV236_002145"
     CDS             5285..7471
                     /locus_tag="IV236_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8772320.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2716 a         1280 c         1424 g         2164 t
ORIGIN      
        1 aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg ttctttttat
       61 gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt tgccaacgtg
      121 acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac ataggattta
      181 tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc atagaattgt
      241 aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga agtagaaaaa
      301 tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat tttgaagata
      361 tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat atgctcgaca
      421 ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc gatgttgctt
      481 tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt tcctaaagga
      541 tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc ttctagttcg
      601 atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt gagaagttct
      661 tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat gattctattt
      721 ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat ataaatggga
      781 atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa acgctggttt
      841 tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc caagataaac
      901 aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg tttcaactgg
      961 tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga atgattcttc
     1021 ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc aatcgcattg
     1081 tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc cagttcgatt
     1141 ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat gagttttaat
     1201 gacagtaaac tacggatctc aggaactttc atattatcca acatacttag attggccgga
     1261 aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga ccgtaatcct
     1321 tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg gtaatcatgt
     1381 ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc ctgttgcatc
     1441 ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt ccaataataa
     1501 accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt caaagctaag
     1561 acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta tgtaaataat
     1621 atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca agtctctaac
     1681 gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac aatgtgaaaa
     1741 tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa atcgtcgatg
     1801 cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc gtaaaccaat
     1861 tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat agcaattaga
     1921 gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga aatcagaatg
     1981 actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat acaaattgtg
     2041 taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc ccattgattt
     2101 ttaccaaatt atagcacaat tagcgtatta tttttttaat ttttcaggga ttttgaattc
     2161 agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag ttaattgttt
     2221 aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt gtaataggaa
     2281 gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag taagaagctg
     2341 agaaaaataa agtaatgaga gaaaaaatta gaaacatgct gatcgccttc tttaataatg
     2401 ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca tagcgaagca
     2461 gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata gtcaattgca
     2521 gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc agacaaacga
     2581 attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa gaaagaaagg
     2641 tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg accgctagtt
     2701 ttccaagatt attcaataaa acttctaagg caaattttac ttggacatac accatatctt
     2761 cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact ttatctttca
     2821 tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg tcttttgttt
     2881 cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc ttcgttttcc
     2941 aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta tgacaagttt
     3001 gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt gatggaggga
     3061 ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt aaatctgatt
     3121 tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt gtgatgatac
     3181 gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac tggcagttag
     3241 ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga gagcataaag
     3301 aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa aacagctttt
     3361 catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact gtttatttga
     3421 aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag gagttacttt
     3481 tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt ttatttctat
     3541 aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga taattgtgat
     3601 cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc ttgcccaaga
     3661 tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc cgctatttta
     3721 ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca aacgcttttt
     3781 ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca tctacgaaga
     3841 tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag aagtaaagca
     3901 gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc cacaaaagaa
     3961 accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga gaatcaaaaa
     4021 aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct actataaaga
     4081 actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca aaatagtgat
     4141 cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa tcgtacaaga
     4201 aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga tccgctacac
     4261 tacaaataac ccgaaagcaa agggaatctt tacaaataaa taatataaaa actaagcaaa
     4321 ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt tgattcattg
     4381 agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg tctgagtgag
     4441 ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa acagatgatg
     4501 acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta gatagtggaa
     4561 aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt tcgttgctac
     4621 cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga ttttatcaaa
     4681 agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg cgggatgctc
     4741 atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata gaacaaacaa
     4801 gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg ttagaagaat
     4861 ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc attctccgtc
     4921 aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa aagcaccata
     4981 ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa gtcctattgt
     5041 acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat atttttgtct
     5101 tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5161 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5221 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5281 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5341 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5401 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5461 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5521 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5581 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5641 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5701 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5761 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5821 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     5881 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     5941 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6001 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6061 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6121 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6181 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6241 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6301 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6361 tcttttctta ttgactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6421 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6481 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6541 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6601 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6661 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6721 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6781 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6841 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     6901 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     6961 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7021 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7081 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7141 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7201 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7261 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtgacgac
     7321 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7381 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7441 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7501 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7561 cttattttcg aagaagctat ttct
//
LOCUS       DACDID010000124         7546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDID010000124 DACDID010000000
VERSION     DACDID010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.126"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..500)
                     /locus_tag="IV236_002146"
     CDS             complement(<1..500)
                     /locus_tag="IV236_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8772321.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAV"
     gene            complement(500..1636)
                     /locus_tag="IV236_002147"
     CDS             complement(500..1636)
                     /locus_tag="IV236_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8772322.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1668..3119)
                     /locus_tag="IV236_002148"
     CDS             complement(1668..3119)
                     /locus_tag="IV236_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8772323.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3163..3927)
                     /locus_tag="IV236_002149"
     CDS             complement(3163..3927)
                     /locus_tag="IV236_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8772324.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3955..4653)
                     /locus_tag="IV236_002150"
     CDS             complement(3955..4653)
                     /locus_tag="IV236_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8772325.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4665..5444)
                     /locus_tag="IV236_002151"
     CDS             complement(4665..5444)
                     /locus_tag="IV236_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8772326.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5460..6404)
                     /locus_tag="IV236_002152"
     CDS             complement(5460..6404)
                     /locus_tag="IV236_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8772327.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6450..7232)
                     /locus_tag="IV236_002153"
     CDS             complement(6450..7232)
                     /locus_tag="IV236_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772328.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKEV
                     TSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATA
                     TYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2122 a         1594 c         1164 g         2666 t
ORIGIN      
        1 actgcatttg gaatcaaagt gaatttatca tttcgaaaca aaaaatcacc tgctactttt
       61 ccacatgaaa attcatgtgt caaaacagac gttaacttac atcgataata ttgctttaaa
      121 agatatttaa aatctctatc tatcgatgta ttatggctat gtgctatccg tatcggaatc
      181 ccagcttttt ttgcagcttt taaaggtaaa taactcatcg catcaatatg agaatgaaca
      241 atctcatatt ctggatgatc cttaaaaaat tgttccatcc atttaaaata tttaggataa
      301 ttttgtggat aaagtctcgg tgcataatag acctttcctc caagacttat tatttcctca
      361 tcataatcac ttttatatgg acgatgtgtc aaaaaatcaa actgaatttt agttcgatct
      421 atattcctat aatagttcat tagcatcgtc tccaaccctg cacgatgcat atcattcaca
      481 cactgtaata ttcgaatcat tatatatttc ccctatacaa atttaatgtt tcttgtacga
      541 ttttatccca attatattta ctacaaataa actcagtagc ttgattcttc aaaactttaa
      601 ccatttccga ttgattgcat gcctcttcta atcttatctt taaatcactt acatccgatt
      661 tcttaaaaat catagccttg tcttctacta cttccgtaca ctctgagata tttgaaacta
      721 tacaacagtt cccataactc attgcttcta acaaacttaa tggcattcct tctaaatcac
      781 ttggaagtgt atagatatac gcattactat ataattcttc taattcctgt ccttgtacaa
      841 atccagtaaa tataattcgc tcatcacctt ttgctaattc ctttagttca tttgcaaatt
      901 catctgtatc agagcttcct ccggctatta taagtttttt atctgtctga acatctttaa
      961 atgcctcaat tagataccga attccttttt cgggaaccaa tcgccccaaa aacaaaatat
     1021 aagaatcttt tgttaatcca tacttttctg taatcaatcc tgctgatttt atttcaattt
     1081 ttttaacgcc atttggtata aaccttgttt ttcttccata tatatcttca aaataatttt
     1141 ggacgctttc actaagtaca attatttcat ctgcaaattt tacagcacac ttttccccta
     1201 acatgatgta tgtactagca agcttgttcc atttttctct ttgatgatct agaccatgaa
     1261 ttgttgcaac acatcttttc ccaaaaagtt ttggcaacca caacatagca catggtcctt
     1321 ctgcatgaaa atgaacaaca tcatattttc cgaatgctgc tctaattcca ccaaatactg
     1381 aagaactcat tgctgcaatt ccttttatat ttaatgtaaa aatagtcttt aattttacac
     1441 ctttatattc tttattttta tgctggtcaa attcctttcc gctaacatga tgaccacttc
     1501 gattatagca agtcacttca tgcccaagtg caaccattcg cgttgccaat tcttctacca
     1561 ctatctcaat tccaccttct cttgatggaa ttcttttatg acctagcata cagattttta
     1621 atttttttct tgccatcacg ttttactcct tctatagtat ctttatctta catggaaccg
     1681 tttctatgaa gcacagtttt gactgtccta aacaaaattc taaaatctaa tcccatactc
     1741 caattcctga tatattctgc atcaagtttt acaacctctt cgaagtctgt gatatcactt
     1801 ctaccactca cctgccacat tccagtaatc ccaggtttca tcgcaattct cgcacgatga
     1861 tgaaaatcat acttctcata ttctgacttc aagcaaggtc taaaccccac caggctcatt
     1921 tttcccatta ggacattata aaattgtgga aactcatcca atgatgtaga tcgaataaat
     1981 tgtccaattc ctgtcttctt tgttccatca ggaagtattt tattgccaat gattcttggg
     2041 tcaaaatcca tcttaaacat gagattatta ctcatcttat tttctttcat caactctgct
     2101 ttgcgttctt ctgcatccat atacatacta cggaacttat acatcttaaa tgtcttacca
     2161 ttttgaccaa tacgttcttg tgcaaagaaa atcggccctg gtgatttgat gtaaataact
     2221 ggtgctacga agataaaaat aacacctgtt gcaatacatc caataattcc agcaagaatg
     2281 tcaatcgttc tctttataaa tgcttgcctc atcgtcataa cattaatact agatgtaata
     2341 acagtataat caccaactcg gccaataaat tgttttccac gtgtactatc agacactttt
     2401 gtaagattca tatgtacagc aagtcccatt tccgaacatc tggcgattaa ttctttggga
     2461 tatggaacac tttcgtcaac attgatgaat acttcatcta cccaattctg taaaagatat
     2521 tccgctgcat tatcagcatt tgcaactact gggacacctc cgatggtagt tccatacata
     2581 tctctgtcta ctacaatcac accactaagt ttatacatgc cataattctt ctctttaaga
     2641 ttacgaacta cattttttgc aatcttttga gatgttacaa taactaacga tacatcttca
     2701 ccgtttttca tgcggtgatg caacaaccct ttccaaagta tacgaattat atatgttaat
     2761 aatccataca ccacccccat tataaataaa gaaactctgg aataatcatt tccatcctgc
     2821 attgtaataa ggtataaact gcacacaagt aacagcataa aagattgctt tatagaagct
     2881 acagcctctt tataatatcc tctttttaaa acatttttat atgcctcaaa taaaaatata
     2941 atcgtaagat tctcaagttc tataaaaatt gccatgtggc gataaatagg aatcacatac
     3001 ggattccagt caccctgtcg cattacatat gatatataaa aagcaagctg gacacaaaat
     3061 aagtctaata aaataaaatc aatatgttta gaccaaccac ttgagttttt tctatacatc
     3121 tctcactctc tcctcatttc ctttttatct acgaataaag aactaaaata aaccaaactt
     3181 cttctttttt acctcttcat agattggata attgatctca tccccgttga tcagatcctt
     3241 tgcgacttgt ttcatctgta ctactttttc cttaccaaac tccttggcaa tctgctcata
     3301 gcactctttt aagtaaaagg tgcgcttaga gaccccgtgt gcatcagaag cagccatctg
     3361 cacgagatta tgtttgacca ttgttttggc tgttttctgg atatctttgc caaatacacc
     3421 aacataactt ggcgcagtaa gttgggccaa acatcccata tctaggaacg gaagcaaatg
     3481 atttgggtct tctcgaaaat gcgcgttgcg ttctggatgg acgatcactg gtatctttcc
     3541 tagttctaac aactcaaaaa aaagccgttc tgcatataac ggcacttcca gtgtcggaaa
     3601 ttcgatcaga agatatgtat catctaaatc tgtaaataaa atttcatctc ggtggatgtc
     3661 ttcgatgagc gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaactg
     3721 tcttttctct aattcggctt gtaacgaagc gactagagaa atgacttgtg atttttcatt
     3781 gctgtatttt ccattattgt ggtgtggtgt gcataaaata tgcgtaatgc cttgctgaat
     3841 cgctttttca gccattgcaa tacttgcttc taagttttct gccccgtcat cgattcctgg
     3901 taggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttctta ttccttaatt
     3961 tccgtagtaa taaccagcat ctttgctgtg ttctgctccg ttgtatacga cacctaagac
     4021 tcgtgcttgt accatatcta acagttcttt tgctttagtc aatgattctt ttcgtgacgt
     4081 attttcccgt acgaccaata aggtgccatc cgtttttgaa gacatgatct gtgcatctgt
     4141 tacggcaacg actggcggca tgtcgaagat cactacatca aatagttgtc ttgcttctgc
     4201 taaaatctga tccattcttg gcgaacttag tagttctgat ggatttggtg gtttaggtcc
     4261 acttggcaga atcgacaaat tgtcgactgg tgttcgttgg atctcatctg ctacacttcc
     4321 tgatgaacta agaacagtac ttaatccgct tgcgttgttc aacttgaacg ttttatagat
     4381 gacgggctta cgtaagtctg catcgactag cagcacccgt tgaccagatt tggcaaaaac
     4441 aacggcaatg tttgctgcag tggtcgattt cccttctcca ggtcctgatg aagtcacaac
     4501 aatcgttttg atttgctgac cagctgcgga ggcaaactgg atattcgtcc gaatcgtacg
     4561 atattgttca gcgattggtg aagagggatt ggtgagtgtg accaaactga ctgcatgagt
     4621 ttgggctgct ttttgtttct gtgttcgtgc cattttcttt cctcctagac tcttgagcgg
     4681 cttctgcgtg atggcgctgt tttatcgttt gtagcttttg gttgtgtctg agctggtcct
     4741 tttttacgga taccagcatt caattctttc gctgacattt ctggaactgt tcctaagatt
     4801 gggaatccta atgtatctgt cacatatttt tcatctttca ctgtccgatc gagtaattcc
     4861 aataagaatg ccagacctac acctaccatc attcctaaca ttaagccagt cattaaattc
     4921 aatttattat ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat gatcgagatt
     4981 ttatcaacat tcatcacatc ttttgcgttc tcttggaaaa cttgtgaagt tgtgttagcg
     5041 atctgttgag cagccactgc attcgtactt attgcctgaa tcgagaacat ttgtgaattt
     5101 tctgcctgat taactgagat ggcatctttg atttgttcag cagtcatttt cacattgtct
     5161 tctgtttcta aacgatcttt gacttcattg agcactaaat cactgacgat catatcttta
     5221 tacgtattga tcatttgcag gttcgtattg acatcattga catttgcagt gtctgattgt
     5281 ggcagtgtca cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat gaaaaatgtt
     5341 acgattcctg agatggccaa gccagcaaac attgagatga tgattattac caagtgtttg
     5401 cgtaatgttt caaaaatttc tttcaagctg attgtctctt ccattttttc tccactttcc
     5461 tatttattct ctagattcat ttgatctttt aattcttttt tcacacgatc aagttcttga
     5521 tcactgactt tttgatagga gatattatct tgcatgaagc cttctccttg cagctgatct
     5581 tgcttaatcg taccaaatgc ctcccgataa tctaaagcaa tttttttcat ttcatcaaag
     5641 ttcatgtctg ttttcatatt cgactctaga gcattcaaaa cggattgata gttgctgaca
     5701 ccgtttagac tcaatacttt tttcgctacg ccttctacga tctttctttg gcgcccttgt
     5761 ctaccgtaat caccatttgg gtcttcataa cgcatacgag aataagataa tgcctgatcc
     5821 ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa agtgaggtca
     5881 ttattgactt cgataccacc aactgcatca acgagttcct ttagcccttt catattgata
     5941 gagacatagt gatcgattgg gatgtctaca tatttttgta cggtatccat cgccatggca
     6001 gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc tacgatgtcg
     6061 acatacgtat cacgaggaat gctgacgatc gttgtctgtt tgtcttcagg actgactgtg
     6121 gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc agtatcaatc
     6181 cccatcaata gaacagagaa agattcttgt ttgtccagat caactggttt cgttcgttct
     6241 ccttgggttc gttccactga ttcgtaagtt ttctcaatag aatgtgagag atccagatat
     6301 aattttgctc ctactcctac aattgctaat actaatacga taagaactgc taaaataatc
     6361 agaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc ctccgtttgc
     6421 taaccttgct tgaatcaacg ttttaactgt caatagctta tattgctata gcccatttgc
     6481 tcagcaacag tttgtgcttt tgttgcgtat gctgcgctta ttttttcatt ccggatctgc
     6541 tctatttgac tgagcaccgt caaatattga tcgtaagtag cggtggcagt cacttgacca
     6601 tcctttaaca tcgtatcgaa taactgatct agttgcgtga cttgcgcgat ctgaatatca
     6661 gcgaatccta gatattgcat aacaaccgtt ttccaaggac tatctttaaa aaagctcata
     6721 ttttcacgta cacgagcgac atctgcactt gtcacctcct tttctttgat gatgacatcg
     6781 tctacagctt gctgccagtt tgatacattt ttagtaaata aatcgttgac agctgtctgg
     6841 atcttcagtt ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg
     6901 attgcaacag cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt
     6961 ttgacagagg atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt
     7021 agaaaaattt tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag
     7081 gtgtcttttt gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc
     7141 tgattattga tatggtatcc aaacaaacag atcgcaatag cacctgcacc taccgccaca
     7201 gcagtcaatg acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga
     7261 taaattaaga tataattcac ttacataaaa atgacagctt tttatttaaa aaaacataat
     7321 taaactaata tattcacaga aatatttgta aacaagtttt ccctatataa tcataccaca
     7381 aactgttttc ttgactaggg caatttacga tttaaaagta aataaaacat taataagata
     7441 aaaattaatc gatttcattt ttatttccat aattttcata ggtgttttta aaagttacac
     7501 aaaaaataga ataacaaaaa aattaacatt gaattaataa tagtta
//
LOCUS       DACDID010000125         7328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDID010000125 DACDID010000000
VERSION     DACDID010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.127"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..690)
                     /locus_tag="IV236_002154"
     CDS             complement(70..690)
                     /locus_tag="IV236_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8772329.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="IV236_002155"
     CDS             complement(766..1203)
                     /locus_tag="IV236_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8772330.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="IV236_002156"
     CDS             1366..2196
                     /locus_tag="IV236_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8772331.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="IV236_002157"
     CDS             2180..3547
                     /locus_tag="IV236_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8772332.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="IV236_002158"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="IV236_002158"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8772333.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="IV236_002159"
     CDS             complement(4418..4861)
                     /locus_tag="IV236_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8772334.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="IV236_002160"
     CDS             4995..5366
                     /locus_tag="IV236_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8772335.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="IV236_002161"
     CDS             complement(5535..6317)
                     /locus_tag="IV236_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8772336.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..7285)
                     /locus_tag="IV236_002162"
     CDS             complement(6314..7285)
                     /locus_tag="IV236_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8772337.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2227 a         1530 c         1265 g         2306 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa attaataatt ttcatagaca attccctcct cacaaaagat taacttctaa
     7321 aatcaata
//
LOCUS       DACDID010000126         7290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDID010000126 DACDID010000000
VERSION     DACDID010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.128"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            128..1963
                     /locus_tag="IV236_002163"
     CDS             128..1963
                     /locus_tag="IV236_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8772338.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2023..2739)
                     /locus_tag="IV236_002164"
     CDS             complement(2023..2739)
                     /locus_tag="IV236_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8772339.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILKGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2966..3508)
                     /locus_tag="IV236_002165"
     CDS             complement(2966..3508)
                     /locus_tag="IV236_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8772340.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3513..3740)
                     /locus_tag="IV236_002166"
     CDS             complement(3513..3740)
                     /locus_tag="IV236_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8772341.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3819..5699)
                     /locus_tag="IV236_002167"
     CDS             complement(3819..5699)
                     /locus_tag="IV236_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8772342.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5888..6502)
                     /locus_tag="IV236_002168"
     CDS             complement(5888..6502)
                     /locus_tag="IV236_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772343.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6644..7204
                     /locus_tag="IV236_002169"
     CDS             6644..7204
                     /locus_tag="IV236_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8772344.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2227 a         1464 c         1206 g         2393 t
ORIGIN      
        1 tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag
       61 aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt tttactgctt
      121 taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt
      181 tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca tcattattat
      241 gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct tgactgctac
      301 cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa tgcgggaaga
      361 gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat tatcgatggt
      421 tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga ttactagtat
      481 tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt
      541 tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa
      601 ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca
      661 aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat
      721 catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa
      781 cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt
      841 tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac
      901 agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc
      961 cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat
     1021 cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat
     1081 aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc
     1141 tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga
     1201 cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga
     1261 ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt cacaattcat
     1321 ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta
     1381 cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca
     1441 aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga
     1501 taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga
     1561 ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata acaacaagta
     1621 tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc
     1681 tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt
     1741 atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa
     1801 tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga
     1861 gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa
     1921 ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaataa actgagcaag
     1981 gggttgtgat aaaagacatg tcacaacccc ttgtttatct cattaatttg cttgtccata
     2041 aacgatttga ttaaagccat ttttcaattc attattcgct gtctcgttga agtaaagata
     2101 attatcggga ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa
     2161 cccgaataga tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc
     2221 tgtgatttga tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac
     2281 tccaccaata gccatcttcc actccccttt caaaatagct tgagggactt taagaccata
     2341 taagtccaca ctcatttgat tcgtataact tttgtattgt tgtcccattg tttggcccca
     2401 tttggtcata aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcaac
     2461 ttgttcgctg ctttctgaag aggaagatga agcctctaca gaagaacttg aagtagatgt
     2521 ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttctgt
     2581 acttgctgca gttgacttgc tctctgtgct agttgttgta gtagttgcaa ctgttgcttt
     2641 cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa
     2701 aaaagctaga ccaattgtta ctaaaatcct ttttttcaat taaaaaactc ctttcgatta
     2761 ctcccacttc tttattctat caaataagca atgtgatttt attgattttt cgtattttct
     2821 catcaatctt tcaaatcttc taaaaactga gaagcacccg ctgatgttgt ctgtaataca
     2881 tcagcgggtg cttcttttat tttaatcttc gtgattttcc aatttacctc acgctttata
     2941 tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc
     3001 ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa
     3061 gttcaacaag caattgttgc aagccatatt tttcttcttt ttcagctaat ttgattgccc
     3121 gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg
     3181 ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta
     3241 aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc
     3301 tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca
     3361 aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gctaatacat
     3421 gccccaccat ggcgccagca gttggtttat gatggtcgat atctgcttgt tttgtttctt
     3481 gaacaaattt ttcttccact atattcattg atttacgcct cttttacttt tgctgattgg
     3541 acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga
     3601 tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc
     3661 tgttgagaaa ccgcatgttc gattttttgc atacagcttg gacaagctag tgtatctaat
     3721 tgcataataa tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag
     3781 attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt
     3841 tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg
     3901 tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgtt gagtaaaaat
     3961 aagacgacac caatggcaat cactacattt tcacgggtat ttaaaacagt tttcttagcc
     4021 aagcggtagg catgaaccaa cgattcaaaa ttagattgca tcaagacaac gtcactcgtt
     4081 tcgatagcaa catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag
     4141 ggagaatcat tgattccatc tccaataaaa gcaacgcgca atccttgttc ttggtatttt
     4201 ttcacaaatt gaaccttttc gtctggtaat aattcagcat ggacttcatc aatgcctaac
     4261 atatcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac taactgtttt
     4321 gctcctgctt gtttcaactt agctaattgt tcagcgactt ctggtcgaat cacatctgac
     4381 acacctatga tttgagtgat ttttttgtcg attgctacaa taatgaccga gctgcctgtt
     4441 tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca
     4501 atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt
     4561 cccttgattg tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga
     4621 gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact
     4681 aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt
     4741 cctttggtca atgtaccagt tttatcaaag acaaaagtat cgattttgga aaaagtatcc
     4801 attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca
     4861 ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatt
     4921 gctaaacgaa aatcacgact aatcagacca acaaccaacg caataagtaa aacggttgga
     4981 gtataatact gagaaaatcg atcaatgaat ttttccgcat gggatttggt atcttgtgct
     5041 tcttctacta attcgataat tttggcaaag gtcgtatcat ctccgacctt agttgcttca
     5101 atcgtcacgt agccatctgc taaaatcgtt ccagagaaga cttcatctcc actgattttg
     5161 acgacttgtc gtgattcacc agtcactgcg gcttcatccg catacccttt cccgtcaatg
     5221 actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc
     5281 acgtcatcga tttccacttc ttcagtcgtt ccatcttctt ggacaacact agcagtagat
     5341 ggggccatct cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt tttttgttcc
     5401 agatagctac caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc
     5461 ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttc
     5521 actgcctgat acgcatggat cataataggt atgaccgcaa taatagaagc tatggctaga
     5581 gcaatgttat agaacaagac aaaaccagta aattttccga taaaaccaaa aataatcaac
     5641 acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc
     5701 tttctccctc ttttcctttc attcttacga gactaagtat agatacttat ccatcataaa
     5761 aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga atacactaca
     5821 aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc caactattgt cgtttctatc
     5881 gaaattttca ttgaatgatt ttcctgtaca gcatacatcg gagatctatt tctttatcta
     5941 agatttttat cttgctaatt ttttccttta gtctttcaaa tccacgtcgt tcatagaatt
     6001 gatacgtaca gccactatct gtaaaaagga atatttcttt tcctttctct cttttttcaa
     6061 attctgttag caacttggta ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa
     6121 aaatgatctc tccatctggc gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg
     6181 ctgtctcgta agctccttta ctttctttag caaatatatt ttgtaaaaaa tcgaatatct
     6241 taacatatat ttgctgtcca aaagaacgat gtttcttttt ttctcctttt atctccgcca
     6301 gcaaaacacc tgccaactcg tcctctgcat acaaagcaag aatttgagtt gctcgattca
     6361 actccatata caaaaaatat cttccgtaaa gattcagcag aagttcattt tccatatatc
     6421 tctcaaagtt cattccttca acagcaaaat agatagcttt tttgtaatct ttcttttcta
     6481 agggtttaaa agttagtttc atcatttcgc ctcctcaact tcaatgattg tgttatactt
     6541 acattataat agacacagtg tctactatct ataatcaatc atctataagt ttgtcgatta
     6601 agcgacagct ttgctttgat atgtcggata aggagatagg taaatgaaaa agacagatag
     6661 acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta tccacattat tagtaaataa
     6721 aaagattcaa gcaatcacta tacgcgaact aacagaaacg gcagatgtac atcgttcgac
     6781 tttttataca cattataagg atatctatga tctatatgat caattagaaa gcaacttttt
     6841 tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac
     6901 tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca
     6961 tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat
     7021 atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta
     7081 ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc
     7141 caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc
     7201 ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg
     7261 aatcttattt agccaaattg tttactgaag
//
LOCUS       DACDID010000127         7068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDID010000127 DACDID010000000
VERSION     DACDID010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.129"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..1677
                     /locus_tag="IV236_002170"
     CDS             46..1677
                     /locus_tag="IV236_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8772345.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1680..2759
                     /gene="uxuA"
                     /locus_tag="IV236_002171"
     CDS             1680..2759
                     /gene="uxuA"
                     /locus_tag="IV236_002171"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8772346.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2833..4260
                     /gene="uxaC"
                     /locus_tag="IV236_002172"
     CDS             2833..4260
                     /gene="uxaC"
                     /locus_tag="IV236_002172"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8772347.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4344..4991
                     /locus_tag="IV236_002173"
     CDS             4344..4991
                     /locus_tag="IV236_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8772348.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5120..6496
                     /locus_tag="IV236_002174"
     CDS             5120..6496
                     /locus_tag="IV236_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8772349.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6507..>7068
                     /locus_tag="IV236_002175"
     CDS             6507..>7068
                     /locus_tag="IV236_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8772350.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRS"
BASE COUNT         2444 a          942 c         1464 g         2218 t
ORIGIN      
        1 ctacaattgt aacggaaact aacatatcag tatggagggg taaatatggt tttgattaaa
       61 gactggcaag attctatcga aggattgaac aaaatgcaag ttcaactacc gtattttaat
      121 gtcgaagcgg tcaaacaaga aggtcagaaa catccagtgt ggcttcattt tggtggagga
      181 aatctttatc gaggatttca tgcagaaatt gctcaatgct tagcagatca aaatgaatta
      241 gaatccggag tagttgtgtg tgagacattt gatgaagaag tagtagaaaa agcttatcat
      301 gactacaaca atgatatttt ggagattgtt atgggtgaag atggtgaact caaaaaaaga
      361 cttttagcag taacagctga tagtttatat tgcaatccaa agaatataac ggactatcaa
      421 agagtggtta aatattttga agatcctaca ttacagttta caacgtttac tattactgaa
      481 aaaggataca gtttaaaaga ttctaaagga caattttttt cggtagtgga agcagatata
      541 aaatctggac ctaccaatcc tcaacatacg atgagtattg tgacatcctt gttgtatagc
      601 aggtttaatg ctggagagtt accgattgcg atggtttcta ctgataattt ctcacagaat
      661 ggtcaacgtt ttcaagatag catttttatg attgcagaag cttggcaaaa aaaaggatac
      721 gtttctgaaa aatttattgc ttatttaaag gataaagaaa aagtgacttt tccttggagt
      781 atgattgata gaattacgcc aaatccttcg gaaagtgtca aaatggattt attgaaggcg
      841 ggtttcgaag acttagatat aattcatact gcgaaaaata ccaatatcgc tgcttttacc
      901 aatactgaaa aagtccatta tctagtcatt gaagatagtt ttccaaatgg tcgacctaaa
      961 ctggaagcgg ctggtgtgat tatgacagat agagaaacag ttgataaagc agatatcatg
     1021 aaggttacga catgtttaaa tccgctccat acggcattag caataacagg ttgtttgttg
     1081 gggtataaat caattcaggc agaaatgcaa gatgatgatt tggttggctt aattaaaggg
     1141 ataggttatg tagaaggctt accagtagtt gaaagtccag gcgttattga tccaaaacaa
     1201 tttattgatg aagtaatcaa taaacgtttg cctaatccct atattccaga tacaccgcaa
     1261 cgaattgcaa cagatacttc tcaaaaagta gcgattcgct atggtgaaac aattaaaaag
     1321 tatgtcgaaa aagacggaaa tgcagaaaat ctaacattta ttcctatcgc tattgctgct
     1381 tggttacgtt atttattggc agtcgatgat caaggaaaaa attttgtgcc aagtcctgat
     1441 cctttattgg tagagttgca ggaaaaattc tcagtattga aattaggtga acaagatcct
     1501 gcaaagattc attgtgcggt acaggaaatc ttagaaaata aggaaatatt tggtttagat
     1561 ttatatcaag ttgagatagg aaagaaaatt gaaaaaatct tttgcgaaat gctagtaggt
     1621 cctcaagcag ttcgtaaaac aatcaataaa tatataacag aagttggagg aaagtaacta
     1681 tggaaatgac ttttagatgg tatggggcaa atgaagaaaa aatctcacta caagaaattc
     1741 gtcagatccc agcaatgaaa ggaattgttg gaacattaat ggatatacca gccggagagg
     1801 tctggccgag agaacgaatt aaagctttaa aagatgaagt cgaagccaat ggccttacct
     1861 tgaaagttat tgagagtgtt aatgttcatg atgatatcaa aattggtttg ccaacacgag
     1921 atcaatacat cgagaattat aaacagacga ttcgcaattt ggcagaattt gggattgagg
     1981 ttatctgtta taattttatg ccagtctttg attgggtaaa atcagatttg gattatcatt
     2041 tagatgatgg gtcttctacg ttagcctata tttcaaaaaa tattcctact gatccagagg
     2101 aaattattag aaaagtagaa aattctacta ataattttga attgccgggt tgggaacctc
     2161 atcggttatc acaaatcaag caattattag aagcctataa agaagttgat gaagcgaaat
     2221 tgcgagagaa ctttgcatat tttctcaaag caattatccc gacatgtgaa gaagttggga
     2281 ttaaaatggc agttcatcca gatgatccac catattctat tttcggatta cctcgtattg
     2341 ttaaaaatcg tgacgattta gaatggttgt gtaatgtagt ggattctcct tctaatggta
     2401 ttacgttatg tactggaagt attgcagaac gaccagataa tgatgtctat gaaattctat
     2461 ctgaattttg tcaacgggat cggattcctt ttgcacatgt tcgtaatatc aaatatttaa
     2521 gtgataatgg actcgatttt tatgaagcgc cgcataaatc agaatatggt tctctagata
     2581 tgtataagat attaaaaaca atgtacgata atggatttaa tggctatatt cgtccagatc
     2641 atggtcggat gatttgggga gaaaccggta gacctggtta tgggttatat gaccgagctc
     2701 taggtgcagt ttatttaaat ggtatttggg aagcgcttga aaagggggcg agtaaataat
     2761 tatataatgg agtctattta gaagaaaaat tttctcttct aaaaactaag ggaaggaaag
     2821 gagtgcttaa atatgttttt agataagaac tttttattag agaatgaaat agctaaaaga
     2881 ctttttcata aatttgcaga acaccaacca atcatcgatt atcattgtca tttagaccca
     2941 gcatgtttgc tagaaaataa gcaatacaag aatttgactc aggtatggtt aaatgataaa
     3001 ggagctggag atcattataa atggcgattg atgcgagcta atgggataga agaagagttg
     3061 atcagtggtg caggtaatga ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag
     3121 gcgccaggta atcctatttt tgaatggtct catttggaat tacaacgtta ctttgatatt
     3181 gatttgacaa tttctgagaa gaatgcacca gaaatttggc gacgtgccaa cgaaaaaatc
     3241 caaacggatt cctatcgccc aaaagaattg ctgaagatga tggatgtgaa agccatttgt
     3301 acaacggatg atcctacaga tgacttgcga tatcatactg agctttctaa acaggataat
     3361 tctttcaagg tattacctac tttccgtcca gataaggtct ggagtatcaa atcatcagga
     3421 ttttcgaact atattttaaa acttagtcaa gcagcgggta taccaatcaa aaactttaat
     3481 gggttgaagc aggcattgag ccaacgagtg gattattttc atcaaattgg ctgtcgatta
     3541 gcagatcacg gtttgaatac ttatttttat gaaatagcag atgaaaaaat actcgacaat
     3601 ctgttaataa aagctttagc aggaaaaacg gatttccatt tatcagaaat tgcacaattt
     3661 gttacaggta ttcaattaca tttgatgtct gagtatacaa gatatggttg gacgatgcaa
     3721 atgcatttaa atgctttaag aaataattca agtatattaa aggaacaaat tggagtagat
     3781 gtcggtggtg attcgatggg ggatcaagct agtttgacac aaaatgttgt agcattgttc
     3841 gacactgctg aaaagagtgg gtgcttacca aaaacaattt tatattcatt aaatccaacc
     3901 gattggcttc cactagcaac agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc
     3961 caattgggat gtgcttggtg gttcaatgat acagctgaag gaatgcgtca acagttgact
     4021 acgatggcgc aacaaagttt gttgtcgaat tttacaggta tgttgactga ttctcgaagt
     4081 tttctaagct atcctcgcca tgagtatttc cgtcgagtat tatgtcaatt actaagcgag
     4141 tgggttaaag caggtcgaat tccagatgat gaaatgtatc ttagtcagat agtggagaat
     4201 atttgttatc gtaatgcgta taattatttt aatttttttg aaatcgaaag tagaaaataa
     4261 tacaaaaaat aaaagcttaa atttatttat tgtaagttag tacaactagc tagagtatgt
     4321 atccataaaa gaaggagata gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg
     4381 ctggagtcat tgcggttgta cgtggaaaaa ataaagatga ggccattaaa gcaataaatg
     4441 caattactgc gggtggaatt aaagggattg aactaacttt tacagttccg aatgctgaag
     4501 aaataatcaa agaaattgct aaaaaatatg ctgatcaaaa agatgtaata attggagccg
     4561 gtacagtttt agatgcgttt actgctcgta ttgcaattat ggctggagca gaatatatcg
     4621 taagtccatc ttttgatcga caaacagcag agatttgtaa tttatatcaa attccttatt
     4681 taccaggttg tatgacaatt actgagataa aggaggcact taccgctgga gtagaaatca
     4741 ttaaactatt tccaggttct gcttttggtc catctgttgt aaaaacgtat caagctccat
     4801 taccatatgt aaatataatg cctactggcg gagtaagtct agataactta gaagagtggt
     4861 ttgaagcagg cgttgttgca gttggtgtag gtgggaattt gttggctcct gttgaaaaag
     4921 gtgattttga agaagtaact cgaatagcta gtaaatatat agataagttt tatgagataa
     4981 agaattatta ggagaaaaaa taaataatac tataaaatgt aaaaaataaa tactaactct
     5041 ttatacttca gagtatagta cgtaagttgg cagttcaaca gaatgaaaac gcttaacaat
     5101 aggaaggagc gcaaattata tggaaagaaa atttggtcta agagacaaaa taggatatat
     5161 gtttggagat tttgggaatg atttcacttt catttttgct agttcttttt taatgatttt
     5221 ttatacaaaa gtattgggga taagcggagc taccgtagga acattatttt tagtagcaag
     5281 atttattgat gcagttacag acataacaat ggggcgtatt gtggatggat caaaaccatc
     5341 taaaactggc aaatttaaac cgtggatttt acggatgagt ggtccagtcg ctttagcaag
     5401 ttttttaatg tatcaaacat caatggcatc agcttctatg agtattaaag ttatatatat
     5461 gtatgtaaca tatatactat ggggaagtat tttttataca tctattaata ttccttacgg
     5521 ttcaatggca tcagctatca cttctgttgc agaagaaagg acagcgttat caacatttcg
     5581 tagtatagga gctaccttag caggtatgtt cattggcgtg ttaacaccaa tttttattta
     5641 tacaagagat agttctggag ctcaaattgt acgtggtgga tcaactttta caattgtggc
     5701 aggagtattt tcagtgtgtg cagttatttg ttacgtaata tgttataaat taactgtaga
     5761 acgggttaaa agtgatgaat cgcagttaaa agtttcattg ttgaaatctt taaaaggatt
     5821 agctaaaaat agagcgttgc tttctataat agctgcagct atttttttgt tgcttgctca
     5881 gcttttaaca atgagtatga acaattatgt atttccagat tattataata atgcacaggg
     5941 aatagtttta atgaacttta tcaatcctat tttgatgttg gtacttgttt cacctgtaag
     6001 tttaattttc agtaaaaaat acggtaaaaa agaattggcg tcagtaggga tgttcttctc
     6061 tgctattgta tatttacttt tatttttgat aaagcctagt aatatgtatg tattcttagt
     6121 tcttacttct gttggatata tggggttagg tatttttaat acagtcattt gggcgaatat
     6181 tactgatgta atcgatgatc aagaagtgaa atctggccaa agagaagatg gaactattta
     6241 tgcagtatat tcttttgccc gaaaaattgg acaagcttta gctggcggtg tcggaggttg
     6301 gactctttct ataatagggt atgattcgtt agcaaaagta caaagtggag aagttttaca
     6361 aaagctgtat aactcttcaa cgttgattcc agcagtttgt ttctttgttg taggattgat
     6421 tttatttttc gtatatccat tatctaaaga aaaagtatta gaaaattata atttgctaaa
     6481 tcaaaaacat aactaggaga aaaaacatga acagttggat attttgtatt gattcagatg
     6541 gttgtgtcat ggatacaatg acttataaac acaatctctt tttcgggcca attgctgcag
     6601 agatttttga tgttcatcat agggaaaaat tcctacagga ttggaataat attaatttat
     6661 attcagagac tcgtggaata aacagatttt taggtttagt taaaacatta gaaaaaaatg
     6721 atgtaccatc gattaatcga ttaaagcact gggtatctgt aacaaaagaa ttgtctaatc
     6781 catctttgaa gagggaaata gaatactctc agtcagagga tttattaaaa gctttaaaat
     6841 ggtcggaaga agtcaatcga cagattagtc aagctaaagg attagataaa ccctttttaa
     6901 gtgctagaga tactataaaa gctctgaaaa aatatgggaa gataattatt gtaagctcag
     6961 ctaataagga agctattcaa gaagaatggg aaagacatga attattatcc ttagttgatg
     7021 aactgtgttg tcaagataaa ggaaaaaaag aagacattat tcgaagtg
//
LOCUS       DACDID010000128         6995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDID010000128 DACDID010000000
VERSION     DACDID010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.130"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..670
                     /locus_tag="IV236_002176"
     CDS             86..670
                     /locus_tag="IV236_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8772351.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            772..1920
                     /locus_tag="IV236_002177"
     CDS             772..1920
                     /locus_tag="IV236_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8772352.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(2009..3634)
                     /gene="groL"
                     /locus_tag="IV236_002178"
     CDS             complement(2009..3634)
                     /gene="groL"
                     /locus_tag="IV236_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8772353.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(3685..3969)
                     /locus_tag="IV236_002179"
     CDS             complement(3685..3969)
                     /locus_tag="IV236_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8772354.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            4194..4856
                     /locus_tag="IV236_002180"
     CDS             4194..4856
                     /locus_tag="IV236_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8772355.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(4932..6224)
                     /locus_tag="IV236_002181"
     CDS             complement(4932..6224)
                     /locus_tag="IV236_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772356.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            6394..>6995
                     /locus_tag="IV236_002182"
     CDS             6394..>6995
                     /locus_tag="IV236_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8772357.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHE"
BASE COUNT         2219 a         1400 c         1244 g         2132 t
ORIGIN      
        1 ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg caaatatatt
       61 ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag acggaaaaat
      121 gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag cagtaatttt
      181 aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag caagtgataa
      241 tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag aagaaaaaag
      301 cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat atacactaaa
      361 tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg cacaagtagt
      421 aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag gtacagatgt
      481 ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa atacaatggg
      541 atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa accaaaaggg
      601 agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta tttttaaagc
      661 aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg cttttagaaa
      721 acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga gatgttaaaa
      781 gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata tgtagatcct
      841 tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc acaagcgtgg
      901 aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga ttacaataaa
      961 tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa gcagcatgtt
     1021 aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa gcacataagc
     1081 gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat tgaagatatg
     1141 tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac tctatctgtt
     1201 atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct agcagttaaa
     1261 atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa aaaatatctg
     1321 gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg cagaaaacta
     1381 cacggcataa tagctgagtt tctatattta actggtttga gatatgggga attgctagct
     1441 ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt agattacaca
     1501 tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc gcaacgtgaa
     1561 gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga caatattctt
     1621 gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg cactccgctt
     1681 gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga attagaaaaa
     1741 agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga attaggcgta
     1801 cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac tctgtctatt
     1861 tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa tagcctttga
     1921 tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga agaaagaacc
     1981 ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt gatggatcca
     2041 ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct tctgtcgtta
     2101 ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact ttagttgggt
     2161 ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg aaaccagtgc
     2221 ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca gcgttttcag
     2281 cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct gttgccacgt
     2341 ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct gtaccaccac
     2401 ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct tcaatgcgta
     2461 attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca gctacaccac
     2521 ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa gttgtttcag
     2581 caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct ccagaacctt
     2641 ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct aagttttcga
     2701 ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca cctgtcaaaa
     2761 tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct tttacagcta
     2821 ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct tcaccatcta
     2881 catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct aacaatggca
     2941 gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg ttttctagaa
     3001 cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca cgatcaaact
     3061 gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg attgtgatga
     3121 caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact ttatcagaac
     3181 ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca gttgagatat
     3241 tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga atgcctagag
     3301 gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt gtcagaacag
     3361 ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact tctgatacaa
     3421 gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg atcgtcacac
     3481 catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt cggcctttag
     3541 gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac atagctgcgc
     3601 gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat tgattatttt
     3661 tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt ttcctgcgac
     3721 gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt cgaacataac
     3781 ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca aacggccttc
     3841 accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag ctaaaacgat
     3901 tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat cacctaatgg
     3961 ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac tcttaacctt
     4021 cgagtgctaa cctacatttt taataatact cattctccct cttttttgca agcttttaaa
     4081 tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat aattgaaatc
     4141 aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat tacatgtctt
     4201 taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc tcacccttga
     4261 tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca actgcagtca
     4321 tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt gaacccttag
     4381 cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat ggattggttg
     4441 gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct gtattgtttg
     4501 gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg gaagcacccg
     4561 cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta ttccgccgca
     4621 gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg atcagctctt
     4681 tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc ctaggtttct
     4741 tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc acacacgtag
     4801 gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta gtataaacat
     4861 gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag cgacaggagt
     4921 atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt ctgtctttcc
     4981 tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa aaaactaatt
     5041 gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa acccgagagg
     5101 atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa tccaaaatat
     5161 aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg aacagccatc
     5221 atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt gaaaaaggct
     5281 ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa aaccgtcata
     5341 aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg atcgagaaat
     5401 tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga ggctaagatt
     5461 tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt gaccatcgca
     5521 gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa aagcacacca
     5581 aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac tccttgaaca
     5641 acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa agtgactata
     5701 acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat cttctctctt
     5761 tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat gatgagccca
     5821 gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat ctgagtattt
     5881 agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg taagcgatcg
     5941 atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa ggctatcaca
     6001 tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt gtctatccac
     6061 tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc aacaataatt
     6121 attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag attcacaaca
     6181 gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta tctccttcaa
     6241 cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca cgaaccatga
     6301 aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc agcaactgct
     6361 tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat tggagctact
     6421 ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga cgtgacggca
     6481 atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa ggatttattt
     6541 gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt caacgctccc
     6601 aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat gcttgaagga
     6661 accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga tacagaaaag
     6721 acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac agctttcaat
     6781 atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac ttatatcagt
     6841 ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt aagtgatgat
     6901 tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta cgcgattgca
     6961 ttagtagatg agatactgga tgagaaacat gaaaa
//
LOCUS       DACDID010000129         6925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDID010000129 DACDID010000000
VERSION     DACDID010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.131"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..610
                     /locus_tag="IV236_002183"
     CDS             137..610
                     /locus_tag="IV236_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8772358.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            607..2739
                     /gene="feoB"
                     /locus_tag="IV236_002184"
     CDS             607..2739
                     /gene="feoB"
                     /locus_tag="IV236_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8772359.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2741..2875
                     /locus_tag="IV236_002185"
     CDS             2741..2875
                     /locus_tag="IV236_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8772360.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2969..3613
                     /locus_tag="IV236_002186"
     CDS             2969..3613
                     /locus_tag="IV236_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8772361.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3800..4993
                     /locus_tag="IV236_002187"
     CDS             3800..4993
                     /locus_tag="IV236_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8772362.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4986..6713
                     /locus_tag="IV236_002188"
     CDS             4986..6713
                     /locus_tag="IV236_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8772363.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2228 a         1222 c         1555 g         1920 t
ORIGIN      
        1 gtataattga cattctcaat taccgagagt attctatgtg agaatgattc tcatttattt
       61 gcgtaacacg taagaaaatt agaacatata atgaatgatt ttaatacatg atttatttca
      121 caagagagga atcaatatgc agaccttagc gcaggcaaag cagaatttcg tttatcaagt
      181 cgaacgaatc gaaacagaag aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc
      241 cggaagtaaa atcgtcgtcg tcaatttatc aggcaacaat gccattttac tggtaaaaag
      301 tcatcgagtg gcagtaagtc gggaaatact aggtcaaatc tttgtcaata aaaagactgc
      361 ttcctctgaa agctggactt ctcttgatca attaaaagca ggagagactg ggatcgtcgt
      421 agcaatccac ggacaaggcg cagtgaagcg acggttaatg gatatgggat tgacgcgaaa
      481 tgtaaaaata gttatccgaa aactagcccc tctgggagat ccaatcgaac tgacagtgcg
      541 agggtatgaa ctgacgttac gaaagtcaga ggccgaacta atcttgatac aaagggagga
      601 aaaagaatga atatcgcgtt agtcggaaat ccaaacagcg ggaaaactag cttgttcaat
      661 ctcttgactg gttcgaatca atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa
      721 gaaggatcgt tgaaaaaaaa tccgaacatt catattcagg acctacccgg catctattct
      781 ttgtctcctt atacaccaga agaagtgatt gcaagagatt atttggttca ggaacagcct
      841 gaactgatca tcaacattat tgatgctacc aatttagaac gaaatcttta tttaactacg
      901 cagttattgg atttagacat tcctatgatc attggtctga atatgatgga tgtcatcaaa
      961 aaagaaggaa aaaaaatcaa tctagaaaag ctttcctatg gtcttggtgt accagtttta
     1021 ccaatcagcg tattgaaaaa gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct
     1081 gcaaaaaagc ggccttctat tcctgttccc tgtacatatg atccacgttt agaagcagct
     1141 ttacatgaaa ttgaagatgt actaggagaa caaagcggca agaaacgctg gtatgcaatg
     1201 aaatattttg aacgagatca gaaggtaaga gaggattacg agatagcagt ttctcagcaa
     1261 aaagagatcg agcaactgat ccagttaaca gagaaactat tggacgacga tagtgagacg
     1321 attctggtca atgaacgtta tgaatttatt acccaactat gtacactctg tgttgtgtca
     1381 aatgattctt tccaattatc gatgagtgac aaaatcgatc agattgcaac gaatcgttgg
     1441 ttagccttgc cgatcttcgc atttgtgatg tggctgatct attatctggc tattcaaaca
     1501 gtaggaacaa tggggacaga ttggatcaat gacacattgt ttggtacatg gttaccagaa
     1561 catgttggca gattgttagc tgaatggcaa gtagcgggat ggatgcagga gctgatcttg
     1621 aacggcatca tcgcaggcgt gggagcagtg ttaggatttc taccgcaatt gattatattg
     1681 tttttatgct tgagctttct ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg
     1741 gatcgacttt tcaggaaatt cggtctttca ggaaaatcct tcattcctat gctgattgct
     1801 acaggatgtg gagtgccagg cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt
     1861 cgtttgacga tcatggtcac aaccttcatg ccttgttctg caaagttgcc gattattgcc
     1921 ttagtagcag gggccttttt tcctcatcaa tcttgggtag ctccttctgc ctattttctt
     1981 ggtatgactg caattatttt ctctggaatc agcttgaaaa aaacacgttt gtttgctgga
     2041 gatacagctc cttttatcat ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc
     2101 ctaagacaaa cctatgaccg cagcaagtct tttgttaaaa aagcaggaac gattatcttt
     2161 gtttctagta tcgtgatctg gttcagctct tcttataatt tttatgctca acctgtttct
     2221 gaagaccaaa gtatcttagc ctttttcggg cgaatcctgg cagttgtttt tcagccgctt
     2281 ggttggggaa actggcaagg aacagtcgca gcgattacag gattggtcgc taaagaaaat
     2341 attattggca catttggtat tttgtttggt catgcggaag tatccgaaaa tggcagagaa
     2401 atctggcaag tgctacaaca cacatatact cccgcagcag cttattcatt gttagccttc
     2461 aatttacttt gtgcgccttg ttttgcggca attggggcta tccatcggga aatgaatgcc
     2521 aagaaatgga cttggatcgc cataggctat caatgcggtt tagcttattt agttagtttt
     2581 gtgatttatc agttgggaca cttattggag ggtggacagg tagcgttagg gacgtatctt
     2641 gctattttac tgatgatagg actcgtttat gttttgttcc gtcagccaaa atccaagaac
     2701 cagttttata ctatattgtc attagaagga gaggaatagg atggcgacat tcttattaag
     2761 tgtgttaatc tttggaagtg caggcgcaat tgtctatact cgtttaaaat ccggtaagaa
     2821 ttgtgatgac tgtcaaacag cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt
     2881 cgttgcaaga ggctattctc cccttttttg cttattttgc tagagcatag taagatgagg
     2941 gaaagtaaaa aaagaaagag gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg
     3001 ctaccagcag attgctgttg atttagccga acgtatcgtt gaaaaccgat ataaagtggg
     3061 tgagaagatc catgcccgct caacacttgc cagtacgtac aatgcttctc ctgagactac
     3121 ccgcaaggct atcaatgtgc tggtagatct tggaatcatg gaagtacggc atggaagtgg
     3181 ggcatttgtt gcttcgaagg aaaaagcaaa agatttccta ttgcaatatc aagatgtaca
     3241 atccatccaa gatacaaaag cagaaatcat ggcaagtgtg gagaagcaac aagatgagct
     3301 gaatcatttt tctcaattat tggatcagct ggtcaatcaa acgaagcgaa tacataagat
     3361 gaatccttta cttccgtttg aattatcttt atcagaaaaa gcaatgcatc tggaccgttc
     3421 aattagcgag ttgaatatct ggcaggcgac tggcgccaca gttatcgcaa tttctcataa
     3481 tgaagaactg cttgtttcac ctggccccta tgctaaattt acggcagggg acatcgttca
     3541 ttttattgga aatgaatata cactacagcg tatgacaaac tttttttacc cggagcatca
     3601 gcttgcagaa tgattgtgtg aaatattaaa aaaatattaa cttggctaaa ccttttattt
     3661 acaaagattt agccaagttt ttttcagttt ggacaagcaa atttctttca tttttgacaa
     3721 agtgactaca tgatgttaac atagtgtggt cacttttttc agaaagaaaa atgaatttac
     3781 ataaaaaagg agaatttgtt tggtaaaagt agaagtaaaa catttaacaa agatatttgg
     3841 taaaaagaca caagccgctt tagatatgat gaatgatcac cagccgaaaa cggagatttt
     3901 aaagaaaacc ggtgcaacgg ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga
     3961 aatcttcgtg atcatggggc tttccgggag tgggaaatcc acattgatcc gtctattgaa
     4021 tcgtctgatt gagccaactt cgggcagtat ctatattgat ggagaagatg tatcgaagtt
     4081 aagcaaagaa gaattgcgag aagtccgtcg tcataaaatc aatatggttt tccaaaactt
     4141 cggtctgttt ccacatcgaa cgatcttgga aaatacagaa tacgggttag aagtccgtgg
     4201 tgtacctaaa gaagaacgac aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct
     4261 ttcatttaaa gaccaatatc ctagtcagtt atctggtggg atgcaacaac gggttggcct
     4321 agccagagct ttagcaaatg atcctgagat cttgctgatg gacgaagcgt tctcggctct
     4381 tgatccgctg atccggcgag aaatgcaaga cgaattactg gacctgcaag ccaatgttca
     4441 aaaaacgatt attttcatta cccatgattt gaatgaagct ttacggattg gggacagaat
     4501 cgccttgatg aaagacggcg aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa
     4561 tccagcaaat gattatgtac gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac
     4621 ggctcaaaac atcatggtac ctgcattgac gacgaatatc gaatctgatg gaccaaacgt
     4681 tgcattgaca aggatgcgta atgaagaagt cagcatgctg atggctgttg acagaaagag
     4741 acacctgaaa gggatcatca ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc
     4801 attgatcgac ttcctagatg aaaatgtgac ggtgattggg aaagacatgg tggtaagtga
     4861 tattttcaat attatttatg actcaccgac tccacttgca gttgtagagg atggaaaatt
     4921 aaaaggtgtc gtcatccgag gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag
     4981 tgaacatgaa taatctattg aactatcaat taccagtggc aaactgggta gaaaatatca
     5041 cagaatggtt tacgacaact ttttctggcc tattttcatt tttacaaacg attggacagg
     5101 cagtgatgtc ggggattaca aatttactgt tagtgattcc agctcctttg tttatcttgt
     5161 tattgacggt agcagctttc tttatttcta agaaacgtcc aggacttacg ttgttcacat
     5221 taatcggtct atggtttatt tataaccaag gtctatggaa tgatttgatg aatactgtca
     5281 cacttgttct tttatctagt gtgatctcga tcatcattgg ggtaccatta gggatcttga
     5341 tggcaaaaag cagtaaagcc caaagtatta tcaaaccgat ccttgacttt atgcagacaa
     5401 tgccaggatt cgtttatttg attccagcag ttgcattttt cggtatcggg atggttccag
     5461 gggtatttgc ctcagtcatc tttgctctgc caccaaccgt tcgttttacg aatttaggga
     5521 ttcgacaagt tcctaaagaa ttagtagaag cttctgattc attcgggagc acaagtcgtc
     5581 aaaaactatt caaactagaa ttgcctttag ctaaaagtac gatcatggca gggatcaatc
     5641 aaacaacaat gctgtcactt tcaatggtcg ttattgcctc tatgatcggt gcacccggct
     5701 taggacgcgg cgtattatca gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg
     5761 gggttgcatt agttatctta gcaatcatca ttgaccgttt tacacagcat ttgaaccagc
     5821 caaacaataa aaaagctgca ggagcaacaa cacagaaaag taaaaaacgt caaggactga
     5881 tcattggtgc tgtagtagta gtgatcttag gtgcgattgg tatagggagt ttttcatctg
     5941 ccaaagaaac aaaacgtatc aacttatctt atgttgaatg ggatacagaa gttgcttcaa
     6001 caaatgtagt cggtgaagta ttgaaacaaa tgggctatga tgtaacgatg acgccattag
     6061 ataattcaat catgtggaaa tctgtttcaa atggcgagtc agatgccatg gtttcagctt
     6121 ggctacctaa aactcatggt tcacaatatg cacaatataa agatcaagtc gaagacctag
     6181 gtgccaattt gacaggagca aaagttggtt tagcagtgcc agcctacatg gatgtaaatt
     6241 cgatcgatga gttaaccgat caggcaggta agaaaatcat aggaatcgag ccaggagctg
     6301 gtgtagttac ggctgctgaa aacacgatcc aaaaatatga caacttgaaa gactggaaag
     6361 tagagacatc ttcttcagga gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg
     6421 aaccaattgt agttacagga tggacacctc attggatgtt tgccaaatat gatctgaaat
     6481 acttagaaga tcctgaaaac ggaatgggaa gcgaagagca gatccatacg atggttcgaa
     6541 aaggattgaa agaagaccag ccagaagctt acaaagtatt agacaatttc cattggtcag
     6601 aaaaagatat ggaaaaagtt atgttggaaa tcaacaatgg aaaagatcca cagcaagcag
     6661 caaaagactg gatcaaagag aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca
     6721 gacacgaaat aaatggagct tccaaaattg gatcaaatat ccaaatttgg aagctccatg
     6781 ttcgtctcta tcttttttgt ctataaaaaa agagaatgcg tagctcatct tcgaaacgtc
     6841 caaagacgcc gacaaactat tatgaattta tgggtgcgag tcaaagagat cgatcgtagg
     6901 tatgaaagac taatgtgatt aaagt
//
LOCUS       DACDID010000130         6687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDID010000130 DACDID010000000
VERSION     DACDID010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.132"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..483
                     /locus_tag="IV236_002189"
     CDS             <1..483
                     /locus_tag="IV236_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8772364.1"
                     /translation="FHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDAISIYK
                     FFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKK
                     PLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI
                     "
     gene            764..1984
                     /locus_tag="IV236_002190"
     CDS             764..1984
                     /locus_tag="IV236_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8772365.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1981..4701
                     /locus_tag="IV236_002191"
     CDS             1981..4701
                     /locus_tag="IV236_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772366.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4685..5629
                     /locus_tag="IV236_002192"
     CDS             4685..5629
                     /locus_tag="IV236_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8772367.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5698..>6687)
                     /locus_tag="IV236_002193"
     CDS             complement(5698..>6687)
                     /locus_tag="IV236_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8772368.1"
                     /translation="LAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLES
                     SNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYK
                     EYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDG
                     DDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPT
                     SYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKI
                     KDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2355 a         1088 c         1287 g         1957 t
ORIGIN      
        1 tttcacgagg tagggagtga attttctatc aaagagctaa actatgagct tgagtatgaa
       61 aaatctattt gttcaatccc ttataataat attgaggaaa ttatagaaaa ggatgctatc
      121 agcatttaca aattttttat ttttattcct aatcgtcaat tgaaagctaa tatatttaac
      181 catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg cacctgtaaa tattgaaatc
      241 gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt tggaatcaat ctacaattta
      301 aagaaacctt tgagaatcgc gatcggagat agtttaaatg acttatctat gtttgaaagt
      361 gcagatatta gttttgcaat gggaaatagt catcaagaaa tcatgaaaaa agctgattat
      421 gtcactgttt caaatgataa agacggagta tctcaagcat ttgaaagaat tcttaccata
      481 taaactaatt aaggtagtat agaacataga attctaaata cggaaaaatg gagcataaat
      541 tccagtacaa atagtacttc ttcagtgtca aacgaagcga atataacata gatatttagg
      601 attgtgacaa aaatcatgtc acaatctttt tttcgaataa acttattttt tgaagtcggc
      661 catttatcgt aatttctttg cataatacga acaaattttc ggttattgat gaaaaaaaac
      721 attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga aatttatcca
      781 tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg ataaaactgt
      841 acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat
      901 aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc
      961 attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga accatatacc
     1021 tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt
     1081 tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa
     1141 aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga
     1201 caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt tgtaccacgg
     1261 ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa agatgcaaga
     1321 aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct taaatgaaaa
     1381 gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac aagcttctac
     1441 atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga ttaaagtagc
     1501 agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga caaaagaggt
     1561 cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt taattgagat
     1621 aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac aatcaggaga
     1681 gctattagac tatttgttag aggaattctc agataggata aatgatttct ttatttggcg
     1741 tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt ctgctaaatt
     1801 gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga
     1861 agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat atcaagaaga
     1921 aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg aggatcagga
     1981 atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgac
     2041 ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc aacgatttat
     2101 caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa aaaggattat
     2161 acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc tgtatatggt
     2221 gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg
     2281 gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac gaaagagtta
     2341 ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca tttgagagaa
     2401 aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca agtattccaa
     2461 aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg ccaaaaatta
     2521 ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa
     2581 gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag
     2641 cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc cagacagcaa
     2701 gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag cactacaggt
     2761 cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta tcagcaaaca
     2821 aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat ggatcagcag
     2881 tcagccagat attattttta tttagagcag gagcaggaac taaaagatct ccagcaactt
     2941 caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga gactagagaa
     3001 acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg
     3061 gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag aaggattgct
     3121 gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca agagatgatt
     3181 gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa tcagtcagag
     3241 atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc aataatcatt
     3301 gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg tgcaggcatg
     3361 attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga tttctctgag
     3421 ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc tcaattagct
     3481 gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca gcaagaagta
     3541 tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact catgaaagaa
     3601 tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg gaaacataaa
     3661 agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga gtttgttaaa
     3721 gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct ttcccgtttt
     3781 atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag
     3841 caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata cagcgaacaa
     3901 ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa
     3961 caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac tccactattt
     4021 cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa agaaaagcaa
     4081 tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa
     4141 gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc tagccggcaa
     4201 aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc cgggacattc
     4261 ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt aagagcagca
     4321 ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact gaaaaatgat
     4381 acactgactt taacagacgg atatacagaa tttcccatct atgagttatc tacaggtacg
     4441 aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc taaccgttcg
     4501 atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg aaagtatcaa
     4561 ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat
     4621 aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt agaaggagca
     4681 aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat ttgtcctgat
     4741 caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca
     4801 agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg aaatcaataa
     4861 atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc
     4921 tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag agcctagttt
     4981 atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattgtt
     5041 gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta ataaatatca
     5101 aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg ccagtggatt
     5161 agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg aatatcctga
     5221 gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa aggtgattga
     5281 actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg gtcatctggt
     5341 attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga
     5401 agatgttctt gtattacgtc atatggtttt agcacaccat ggcttattag aatatggctc
     5461 acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca atatcgatgc
     5521 ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg
     5581 gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat
     5641 ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg ttttctttta
     5701 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
     5761 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
     5821 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
     5881 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
     5941 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
     6001 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
     6061 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
     6121 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
     6181 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
     6241 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
     6301 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
     6361 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
     6421 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
     6481 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
     6541 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
     6601 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
     6661 aacagccaac gcactagtag ctgccaa
//
LOCUS       DACDID010000131         6655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDID010000131 DACDID010000000
VERSION     DACDID010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.133"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..835
                     /gene="sufC"
                     /locus_tag="IV236_002194"
     CDS             62..835
                     /gene="sufC"
                     /locus_tag="IV236_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8772369.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            848..2134
                     /gene="sufD"
                     /locus_tag="IV236_002195"
     CDS             848..2134
                     /gene="sufD"
                     /locus_tag="IV236_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8772370.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2131..3366
                     /locus_tag="IV236_002196"
     CDS             2131..3366
                     /locus_tag="IV236_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8772371.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3353..3823
                     /locus_tag="IV236_002197"
     CDS             3353..3823
                     /locus_tag="IV236_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8772372.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3828..5219
                     /gene="sufB"
                     /locus_tag="IV236_002198"
     CDS             3828..5219
                     /gene="sufB"
                     /locus_tag="IV236_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8772373.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5314..6456)
                     /locus_tag="IV236_002199"
     CDS             complement(5314..6456)
                     /locus_tag="IV236_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8772374.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2098 a         1310 c         1414 g         1833 t
ORIGIN      
        1 cgcttcattc tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg
       61 catgtctgtc ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa
      121 aggcgtaaac ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac
      181 aggaaaatct accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg
      241 cgaaattttg tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact
      301 tggcttattt ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt
      361 catgcgtgca gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt
      421 tttgaaaaag ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg
      481 ttatctgaac gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt
      541 gatgttagaa cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc
      601 tttgaaagtc gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat
      661 catcacccac tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat
      721 ggaaggccga gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg
      781 ctacgctggt atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg
      841 agcaagaatg aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt
      901 aagtaaaggc gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga
      961 tgaactgcca ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt
     1021 gaaagaaaca caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga
     1081 caatcctgtg cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt
     1141 agcagaaaaa ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact
     1201 agtccaagaa tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact
     1261 ccatgtagct tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga
     1321 tgaaccactc gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca
     1381 tgtgttgatc gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac
     1441 caaagaacaa gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg
     1501 ttcaaaaatc aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa
     1561 tcgccgtggg cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga
     1621 cggtcatgtg atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt
     1681 caaaatcgtg gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa
     1741 taaagcccct catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac
     1801 attgaccttt aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca
     1861 agaaagtcgt gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt
     1921 gatcgatgaa aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga
     1981 agaaatgtac tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat
     2041 tcgaggattc ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt
     2101 agtcgaagta atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat
     2161 tttcctatct tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg
     2221 acgacccaaa agccaaaaca agtactccag gcattagcgt actattacga acacgataac
     2281 gccaatgttc atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa
     2341 gcaagagaaa aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact
     2401 cgtggtacga cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa
     2461 gcaggagacg agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag
     2521 cagttggcaa aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc
     2581 ctggatatgg aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct
     2641 catgtatcca atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat
     2701 gaaaacggcg gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat
     2761 gtccaagcac tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca
     2821 ggtattggtg ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt
     2881 ggtggcgaaa tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg
     2941 aaattcgaag cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat
     3001 tatttgcaaa aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat
     3061 gtgttgccaa aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat
     3121 tttcggacag gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca
     3181 gctttagaca tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg
     3241 aagtaccttg aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa
     3301 gacgccgacc gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact
     3361 atctaaatta gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca
     3421 tcgtggaagc ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga
     3481 tgtgatcgaa ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg
     3541 cagtggctgc tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa
     3601 atcaatagaa gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga
     3661 agtcaaagat cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc
     3721 cgctcggatc aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga
     3781 tggtaccgca gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag
     3841 aattagaaga atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag
     3901 aaggcttgac agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga
     3961 tgcttgaatt ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg
     4021 gccccgacct ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca
     4081 aacccgctcg tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg
     4141 gtataccaga agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag
     4201 tcgtgtacca taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg
     4261 actctgcttt gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc
     4321 caaccgacaa caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt
     4381 acgtgccaaa aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg
     4441 aaaacacggg gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact
     4501 atgtcgaagg ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag
     4561 aaatctttac aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca
     4621 atgtgtacaa cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga
     4681 tcgacggaaa cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa
     4741 aaggtgcacg aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata
     4801 cgggtgcaaa aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa
     4861 tttcaaaaga cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg
     4921 cgggttcgat ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg
     4981 atacgatccc attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag
     5041 tctcaaaaat ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg
     5101 aagcaactga aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg
     5161 aatatgcagt cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa
     5221 cctgttatag gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt
     5281 atactagaaa tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa
     5341 gtcttgacct gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac
     5401 tttactattt tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa
     5461 taataaacta gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt
     5521 tttcaaaata tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac
     5581 tgttgtaaaa acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc
     5641 tttttgctgt aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac
     5701 tcgtctagaa gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc
     5761 aagagtttta ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc
     5821 gagtacttca ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt
     5881 catgttacca aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa
     5941 tttacgattt ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt
     6001 gtcaatatat tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata
     6061 atcgtaactt tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga
     6121 gatattcgat agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac
     6181 tgagattgcg attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata
     6241 caggtcttca aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct
     6301 gtactcgtca taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt
     6361 tattttttca ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg
     6421 accgtttttc tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca
     6481 aggagtactt accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc
     6541 ccaattgatg aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct
     6601 tcttcgggat aagacatgtc tttcaattga ataggtagcc attcatctac cattc
//
LOCUS       DACDID010000132         6481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDID010000132 DACDID010000000
VERSION     DACDID010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.134"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            142..885
                     /locus_tag="IV236_002200"
     CDS             142..885
                     /locus_tag="IV236_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772375.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            878..2458
                     /locus_tag="IV236_002201"
     CDS             878..2458
                     /locus_tag="IV236_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8772376.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2709..3188)
                     /locus_tag="IV236_002202"
     CDS             complement(2709..3188)
                     /locus_tag="IV236_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8772377.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3204..3956)
                     /locus_tag="IV236_002203"
     CDS             complement(3204..3956)
                     /locus_tag="IV236_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8772378.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4073..4143
                     /locus_tag="IV236_002204"
     tRNA            4073..4143
                     /locus_tag="IV236_002204"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4105..4107,aa:Gly,seq:ccc)
     gene            4504..5229
                     /locus_tag="IV236_002205"
     CDS             4504..5229
                     /locus_tag="IV236_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8772379.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5484..5750
                     /locus_tag="IV236_002206"
     CDS             5484..5750
                     /locus_tag="IV236_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8772380.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5747..6178
                     /gene="ruvX"
                     /locus_tag="IV236_002207"
     CDS             5747..6178
                     /gene="ruvX"
                     /locus_tag="IV236_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8772381.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6203..>6481
                     /locus_tag="IV236_002208"
     CDS             6203..>6481
                     /locus_tag="IV236_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8772382.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFN"
BASE COUNT         2105 a         1125 c         1284 g         1967 t
ORIGIN      
        1 agtgttttcc tgtagaagag aacaacccaa gtagcagcct agtcgtttct atcctttttt
       61 gttatgcact ctttttgaca tttactccac caataacgaa gggtacaata aaaagtaata
      121 acaaacaaaa ggagtacaga aatgattgaa ttcatcaatg taagtaaaag ttacgggaaa
      181 aaacaagcat taaaagactt gtctctctcg ataagacaag gggaaatttt tggattttta
      241 ggacataatg gtgccggaaa atcaactacg attaaaagtt tagttagtat tattgaacca
      301 tcatcgggaa cgattttagt agatggaatg aaattgacgg aaaatcgatt gagtatcaaa
      361 caaaaaatcg gttatgtgcc tgattcccca gatatttttt tacaattgac ggctggagaa
      421 tattgggact tgatttcagc tgcctatgaa ttagacacac aaaaaaaaga aaagcgttta
      481 gcagaattga cagcgctttt tgatatgtat agtcatcaaa atgaaacaat cgcaagtttt
      541 tcacatggaa tgcgtcaaaa aacgattttg atcggcacgt tgctgccaga tccagatatt
      601 tgggtattgg atgaaccttt gcaaggactt gatcctcaag cagcatttga tttgaaagaa
      661 atgatgaagg ctcatgccgc aaaaggaaaa acagtcattt tttctacaca tgtattggat
      721 accgcacaac agttatgcga taaactggct attttgaaga aaggtgaatt gatttatcag
      781 ggggcagtta gtgaattatt gaatggctca cctgatgaaa cgctagaaaa gatttatttg
      841 aaaatggctg gaaggcaagc gacaggagat ggaccgtatg tataagcgac aattattaga
      901 attaattcga gttaatcttt tatatgttaa tccacagctc acgaataaag caagagagaa
      961 aggaaaaaag ggaaagcgat taactatcta tcttttgtct caaaatcttt tttcagggct
     1021 gattttttta ctgatttacg gatttgctat gtttgctatg aactttgtta aattgccagg
     1081 tttctttact tattacgtgg cactttttgg tatgttaggt ttaactcaaa gtatatcagt
     1141 catttataat atcttttttg aaggaaatga cctgcaagta tttttgcctc tgccttttgg
     1201 acaagaacag gttttttcag caaagatttt ggtagttgct ttgaccatca ttccttttgt
     1261 ttttccgctt ctagtcatgt ttattttaac cggatggcga gctgatctag ggattttgct
     1321 tactatcatt atctcaatcc ttttgttttc tctatttttg attattcttt tttgtctggc
     1381 tagtttgatt gtttttggtt tggctcgaac agcagtgttt aaaaaacata gaaaaacagt
     1441 ttctagtatc ttgcttggca gctcgatgct gatcgcattt gctggaatca tgtggatgaa
     1501 tcatcaaacg gggactattg aaaatgagtt aacagatcgg catccgattt cgatattatt
     1561 gccattctat gatgctgcca gtcagccact ttctcaacaa ggtttattgg gttggggaat
     1621 actagctatc acggctctgg gatttttcct tgctgtaaaa cagctgatag taccaaagct
     1681 tacagagcaa ctgactgatt caaacagtga gttcaaaata aaaagaaccc ataaaaagaa
     1741 ccaaaatgtg catcagcttt tatttagtta caatgctcaa ttagtaaaag aacctaattt
     1801 gattatgcaa gttctttctt cttccttgtt aactccaatc atttttattc ttgcttttgc
     1861 cctaggaggc gaaataaaat taactgattt agataataag ttaatcggtg ttgttttcct
     1921 ggtagggatc gcattggctt tcttaacagt taatcagact tcttttatca gcaatcttat
     1981 ttctttggat caagaaaatt ttctgtttat ccgttctttg ccaatctcta tgaaccaata
     2041 tttaaaagaa aagttcagat tcggatggat attgcagagt tttttaactg gtgggatcgc
     2101 attattaagc ggactgtctt ttcagctgcc tttgtttttt atccctagct tgctatttgg
     2161 ttctttattc ggctgctact tattgtcact acgttatttc gctagagatt atcggttgct
     2221 gctgctgaac tggaccaata ttaaccaatt atttaacaga ggttcaggaa gtttgggttt
     2281 agtcgcaact atgatgggtt cattgatcat cagtattatt cttatcgtct tatacggaat
     2341 gttggctttg ttttatccat tttggttagt gaatattcct gtgatcggtt tagttctaat
     2401 actttccgct ctatggatcg tttattatca acgaacgttt tggaaaaaat ttgaataaag
     2461 aagaggggcc gtgacaaaaa tcatgtcgca gccccttttt tacagataga gggtgttcaa
     2521 aagtcagttt ctcagtatta agtcaatttt atgtaaaaat gtgagaagct atttttacaa
     2581 aacttttttt aatactcata attacttctt attgctcctg cggttacttg tcgcaatgtt
     2641 tgtgagatat tttgtcatcg ctcaaggctg aacgtctctt tcacaacctt ttctttgtta
     2701 cacttcgttt atttaaaaag ccgttttttt cgtttcatca tgattgcgtc acgctttact
     2761 ttgaaattac ggatgttatg gttaaagact tctgataata caagacctaa agcgattgct
     2821 ccagcaatca taattgcttg gaccgcataa tgagccgctt ctcgatagga ttcagtaacg
     2881 agatttcgaa ccgcttgata agctaaacca ccagggacta gaggaaccat cccgggaata
     2941 ttgaaaatcg ttacgggcat tttcaaacgt ttagagaaaa gatcgctgat gaatgctact
     3001 cctaaagaac caagcaatga accaaaagca gcagatccgc ctatttgaac acagatccag
     3061 tagatcatcc agccaaaagc accagaaaga ccacaacaaa tcaatgaacg cctaggtaca
     3121 tttgtaataa cggcaaatgc cgcagtcgct aaaaaactaa aagaaaactg aataaatatc
     3181 gtccccatac ttgtgttcct ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa
     3241 ccgatcatcg tagcagtcat catcgcttct gttccgcgag aaactcctga gacataatgt
     3301 ccagctagca aatcacggat agcgttcgta atttgaactc caggaactaa aggcatcaca
     3361 catccgataa tgattagatc ctgattgctt cctaagccaa aacgtacgct gtagatagcg
     3421 gctatgccaa tcaataaaga agataaaaat tccgataaaa atttgattct aaaaaatttc
     3481 aaactatata gataagctgc gtatcccaat cctccaatga ctaaagtcac aggtaaatcg
     3541 gtcatctttc cgccaaataa caccataatt gttccactga tcactgccgc gctaacgatt
     3601 tgcaaccaga caggaaagaa agaacggtct cggtcgattg cttgcagttc gcagtaaagt
     3661 tcttctaatg ttagctctga agcaacatat ttacgtgaaa gatcatttac ttttactact
     3721 ttttctaaat taatcgaacg gttggtaatt tgttccattc ggatggtcga ggtacgatca
     3781 agaccaataa agagaccggt ttgagtgaca tagctgacca aacggaaatt tccagaagct
     3841 aaagcaattc gactcatcgt atcttctacc cgatacatct ccgcattgct ttccatcatg
     3901 attttcccag cgagtaaaca agtgtccagc aacagatcta attcttttcc cgtcatagca
     3961 tcttctccaa taattgattg attagtttta ttttactaca aaacatacca gaagcgtaaa
     4021 gaataaaaaa acttgaggaa ggaataaaag aggcgtatac tgtttttgta ttgcgggtgt
     4081 agtttagtgg taaaatccca gcttcccaag ctgatgtcgc gggttcgatt cccgtcaccc
     4141 gcttaaaata ggttgtgaaa aagctgctct gcaacaagta ataagaacag ccaagcaaaa
     4201 acaacttctt atgtttttaa cttggatgga cttattaccg cagatgcaag cttttgcgca
     4261 ccgtttacca agcggttgtg aaatcagtgt attgtcttga gtatgaagaa aatttttgaa
     4321 taataataaa taccttacag atagagtggt gactgattag agacgaggaa tccgtctctt
     4381 ttttgtattt ttaagccatc ttaaaacagg ttttattttt tgctgtttct atttctttct
     4441 agtatttatt tataggggaa catatgttaa gatttaggga gctgttagta aaggagtaaa
     4501 gaaatgagca aaaaaacaac caaggcgatc gtggggatcg tcgctgctgt gattgcaggg
     4561 gcattcggtg tttccaccac acaaaaagat tcagatatct ttcagcagat tacaactatt
     4621 tttgattcca caccacaagc ttctcaagta gcggatgtgc cagaatatga tggaaaacat
     4681 caagaaatag aaatcaatgg gaatcagccg gaatttacaa aagaggatct tagcctttcc
     4741 aaaggaacat gggaaagcta ttcaaatatt gataaattga acagagtagg acaagccaat
     4801 gctatgcttg gaaaagattt gttccctact gaaaagagag agcctttgta tattgacccg
     4861 acaggttgga aacaaaagaa attaagtgac gggcaatggt tatataatcg cagccattta
     4921 attggtttcc agctgactgg tcaaaataac aacattaaaa acttgatgac agggacacgt
     4981 tcgttgaatg ctccatacat gcttgctcac gaaaatgata ttgcggcata tatcaaagaa
     5041 acaaatcatc atgtacgcta ccgtgtcact cctcattttg aggggaatga actggtagcc
     5101 aggggagtgc aattggaagc agaaagcatt gaagacaaaa aaattgaatt caatgtcttt
     5161 atctacaatg ttcaagatgg ctacacgatc aattatgaga caggacaagc aacaaaaaca
     5221 aaaaattagt agcagctatt cgaatttttt atgaatatta tgaaaaaatt gtgtaaaaac
     5281 atacattcaa agaaatatag cgtatacttt atgaaaagag tacttttgat aaagctacag
     5341 aaaaaaggaa aaaaacatac aaatagtgtt tcttaattta ttcggaagtt tggaatattt
     5401 gtttgtagtt ttccctttct tcgagtaaaa taaggttgta gaaggtaagc tggaattacc
     5461 ttgcgggaaa gaggtgtaga atcatgggtt ttacagatga aaccgtacgt tttggttttg
     5521 acgatagtcg caaaaaagaa gtgagcgaaa cattagcgat tgtttatcgg gctttggaag
     5581 aaaaaggcta caatccaatc aatcagattg tcggctactt gctttctgga gacccggcct
     5641 acattcctcg ttatcgtgat gctcgaaatt tgattcgccg tcacgaacgc gatgagatca
     5701 tggaagtaat tgtcaaggac tacctctcaa atcatggagt gaatttatga gagtaatggg
     5761 cttggatgtc ggttctaaga cagtaggtgt tgctatcagt gacccctttg gctggacagc
     5821 tcaaggagta gagatcattc ggatcaatga agcagaagaa gagtttggct ttgatcggtt
     5881 gaaggaatta gtagcacaat atgaagtaga ccggtttgta gttggattac ctaaaaatat
     5941 gaacaattcc atcggaccaa gggcagaagc ttccatgtca tacggagaaa agatcaaaga
     6001 actattctcc ttgccagttg attatcaaga tgaacgatta accactgttc aggcagaacg
     6061 tatgctagta gagcaggcaa atacttcaag agccaaacga aaaaaagtca tcgataaatt
     6121 agcagctgtg atgatcttac aaaattattt agacggctca ggtaagcaat tattttaata
     6181 ataaaagaaa agaggtaatc ttatgacaga acataaccat tcccacgacc atgaaggaca
     6241 cgaacacatc acattggtgg atgaccaagg aaatgaaaca ttatatgaaa tcctactaac
     6301 agttgacgga caagaagaat ttggacgtaa ctatgttctt ttatatcctg ctggtgtttc
     6361 agaagatgaa gatgtagaat tacaagctta tgcatacatc gaaaacgaag atggaacaga
     6421 aggcgagtta gaacaaatcg aaacagacgc agaatgggac atgattgaag aagtattcaa
     6481 t
//
LOCUS       DACDID010000133         6285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDID010000133 DACDID010000000
VERSION     DACDID010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.135"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV236_002209"
     CDS             complement(99..1229)
                     /locus_tag="IV236_002209"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8772383.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1787..2860)
                     /locus_tag="IV236_002210"
     CDS             complement(1787..2860)
                     /locus_tag="IV236_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8772384.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRK"
     gene            complement(2857..3684)
                     /locus_tag="IV236_002211"
     CDS             complement(2857..3684)
                     /locus_tag="IV236_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8772385.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3685..4491)
                     /locus_tag="IV236_002212"
     CDS             complement(3685..4491)
                     /locus_tag="IV236_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8772386.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4503..5588)
                     /locus_tag="IV236_002213"
     CDS             complement(4503..5588)
                     /locus_tag="IV236_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772387.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5605..6147)
                     /locus_tag="IV236_002214"
     CDS             complement(5605..6147)
                     /locus_tag="IV236_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772388.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1901 a         1323 c         1002 g         2059 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaattc taagaatagg tcattataga tgcctaggta tttcgatcct agatctttgt
     1861 agacttcaag atgggagatc gtgtcatcag atggatagaa ttgttcgtcc gacgaaatgt
     1921 cttttggcaa taacgctttt gcttcttcat ttggtgtaga ataaccaata tactctgcat
     1981 tttgtgccgc attttccggt tctagcataa agttgatgaa atcataggca ccttctttat
     2041 ttttcacggt ttttggcata acgatattat caaaccaaag attggatcct tctggtggga
     2101 tcacataatg taaatgttca ttttcccaca tcatatcggc agcttcgcca gaaaatgtca
     2161 ctgccacgga ggcttcttca ttgatcatat acattttgat ctcatctgcc acgattgctt
     2221 tgacatttgg tgttaaatga ttcaatttat ttgctgcttc tgtcaactgt gccatattct
     2281 tactattcaa agaatagcct aaactattca aagaaagccc cataacttct cgtgcaccgt
     2341 caatcagcat caaactgtct tttaattccg gcttccacaa atcatcccaa tgcttaattt
     2401 gatttgcatc aaagaattta tcgttatata cgatccctaa agttccccaa aaatatggaa
     2461 tcgaatactg attatctgga tcaaaatcca agtctaaaaa tcgtggatca atattattta
     2521 atcctttgat cttcgaatga tctaatggaa gtactagttt ttctttgatc attttttgga
     2581 tcatatattc acttggtata gcgatgtcgt agtttgtccc accttgctgg atttttgtga
     2641 acatcgcttc attagaatca aatgtttcat aattcacttt gtatccatat tctttttcaa
     2701 atttcgtgat caaactagga tcgatataat ctccccagtt atagatggta agtacctttg
     2761 ctccactcat gccacttgtt ttttcaagtt gacgaacacc aagcagcagc accgcaataa
     2821 tgatgagaat acctaaaaac agagattgta attttttcat cgcagccgtg ccaccccctc
     2881 gcgtactctt ctgtttttct tcagccgttt tggacggttt tctttactaa taaagtagta
     2941 tccaatgacc aatatcatgg agaacaagaa aactaacgca cttagcgcat tgatttctaa
     3001 actgattcct tgtcttgcac gagaatagat ctctactgag agcgtcgtaa agccatttcc
     3061 tgtgacgaaa aatgtaaccg caaaatcatc aagagaatag gtaaatgcca taaaatatcc
     3121 agctatgatt cctggagaaa gataaggcaa aatgatattt ttgactactt ggaaattatt
     3181 agcaccaagg tctctagccg catcgaccat tgaatcgttc atttcctgca attttggcaa
     3241 gaccatcaag acgacgattg gaatgctgaa agcaatatgg gaaagcaata cactctgaaa
     3301 acctaatccg aatcctagta gtgtaaagaa aatcaagaaa ctagcaccga taataacatc
     3361 aggagagact aataagatgt tattcatgct caacaacgct gtccgtgcac gtcgtttttt
     3421 cgtgtaatag attcccatag ccccaaatgt cccgatgatc gtggcgatca atgcagataa
     3481 gaaagccagc atgaacgtat tcaatacgat aatgatcaaa cgtgtatctt caaaaacagc
     3541 ttcataattt tcccaagtaa atcctgtaaa gctgttcatc gttcctcctt cattgaagga
     3601 ataaaaaatc agatagaaga tcggtgcata taagaataaa aagacgatcc ctaaataaag
     3661 atacgaccat ttaaattttt tcatttatga gcgacctcct tttttcttct cgcctgtcag
     3721 gagcatcacg atgaacatag caatgattaa aatcacaccg attgtagaac ccatccccca
     3781 gttttgtgta accaagaaat gctgttcgat agccgttcct aaagtgatca cccgatttcc
     3841 gccaatcaga cgagtcagca tgaataaaga aagagaagga ataaatactg cttggacacc
     3901 acttttcaca ccatttaatg ataacggaaa aatcactcgt cggaaggtct caaagctatt
     3961 cgcgcctaaa tctctactag cactgatcaa agaaggattc atctcttcca atgcattgaa
     4021 aattggcata atcataaatg gaatctcgat atacgttgcc acgaacagaa aactgaagtc
     4081 tgtgaataat atctgtttcg gcccgatccc gataaaatca aagaaatgat tcacgctgcc
     4141 gtgcacactg aaaatcccaa taaatgcgta agctttaagc agcagattca cccatgtagg
     4201 caaaataatc agcatcagcc ataattgctt gtgcttcagt tttgttaaaa aataagcagt
     4261 cgggtaactg atgatcagcg tgaaaaacgt gatcaaaaat gcataccata cagaattgat
     4321 cgtcatagaa agcgatgtcc ccgacgtaaa ataagataga taattattca gcgtgaattg
     4381 cccattcata tcaaaaaaag attgataaat gatcattaat acaggcgcga tcacgaataa
     4441 accgagccac aaaatataag ggacggaata gatcctgcgc attgttttca ttcttgtccc
     4501 ccctattctt cgtagctgtc aagacgtgca tcgaattctt cttcagactc gttgaaacgc
     4561 atgacatgga tatcttctgg ctcaaaggcc aatcctactt tgcttccttc tttagctttt
     4621 ttcgttgaat ggaccatcca ttcattttgc tgttcatcat aacagatgat ttcataatgg
     4681 acaccacgaa acagctgagt atcgaccgtt acgactagtt ttcctttatc tggtgtcgtg
     4741 atcgtcaaat cctctgggcg taaaacaact tcaactggtt catttagacg catcccgcca
     4801 tcgacacatt caaattgctt accaacaaac tcaactagat tatcttcgat cattacaccg
     4861 tccacgatat tgctctcccc aacaaaatca gcaacgaaat gattgattgg ctcatcataa
     4921 atatctacgg gtgtaccact ttggacgatt ttccctttat tcatgacaaa aatctcatca
     4981 ctcattgcca acgcttcttc ttggtcatga gtaacaaaaa taaacgtgat tcctaatcgt
     5041 tgttgcagat cccgcagttc atactgcata tctgtacgta gtttcaaatc aagcgccgat
     5101 aacggttcat ctaatagcaa tactttcggt tcatttacaa ttgctcgtgc aatcgctaca
     5161 cgctgtcttt gaccaccaga catttcacta atctcccgtt tttcgtatcc aggaagctgg
     5221 accatacgca aagcatcttt taccttcttt tcgatatctg atttggacat tttcttgatc
     5281 ttcaatccaa aagcaacgtt atcaaaaaca ttcatatgag gaaaaagtgc ataatcttgg
     5341 aagactgtat ttacttgtcg cttattagct ggcaaatcat tcaaccgttt cccttcaaaa
     5401 tagatgtccc cttctgtagc ttcggtgaat ccagctataa tacgtaggat cgttgtttta
     5461 ccacagccgg aaggaccaag cagtgtataa aattttcctt gttcgatctc aaaacttact
     5521 ttttttaata ctgtttcatc atcatagcgt ttcactactt gatcaaatga aatgattggt
     5581 ttccccacgt ttttaccccc tatattacaa atatgattgc gttgcaacaa tcaatacttt
     5641 tgttcgtttt ttgagatgat tccttaattg atgttgtttt gtcgcttgat aatagatcga
     5701 ctgtcctgct tttgccacat agaccgtctc tcctaagctt aactctaatt ccccttctaa
     5761 tacaaaaata aacgtttcag ataatgaagg ttcaaacgtc ttatattctc ctgcaccttc
     5821 aaaagtcagc aagacaggct ccatttcttt ttcattcgaa tcagccacga gccattccaa
     5881 ttcatacccg ttttcttcat cgaaataaac cgtatgatct tcttttgaat aaaccacttg
     5941 cagattcgct gcttcttgat gaaaaaattc ttctggagtc acacctaaga cttcaagaat
     6001 agaaaagaat gtttccattg atggtgaact taaatctcgt tccaactggg aaatataccc
     6061 ttttgacaaa tctgtgcgtt cgcctagttc ttcttgtgtc aaattctttt ggatgcgcaa
     6121 atttcttaac ttctcaccaa tttccataat ttcctccgaa tcgaagtttt ctaatagtaa
     6181 acttcctgtt tactaccact cgtattagta tacgaaattt attgaaaaag gcaacccttt
     6241 gtaaaatttt ttttgctgaa tatttcttta tcttttggaa taaat
//
LOCUS       DACDID010000134         6264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDID010000134 DACDID010000000
VERSION     DACDID010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.136"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2455
                     /locus_tag="IV236_002215"
     CDS             <1..2455
                     /locus_tag="IV236_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8772389.1"
                     /translation="TQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVME
                     QVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDE
                     VDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVE
                     ENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECP
                     LRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESN
                     QEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDE
                     IEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENE
                     AMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIAS
                     IVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAII
                     KLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTL
                     TSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLK
                     LFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLE
                     HIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVS
                     IQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQ
                     VLPTYEILLPSSLKSYFRL"
     gene            2637..2876
                     /locus_tag="IV236_002216"
     CDS             2637..2876
                     /locus_tag="IV236_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772390.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2892..4265
                     /locus_tag="IV236_002217"
     CDS             2892..4265
                     /locus_tag="IV236_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772391.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4269..5903
                     /locus_tag="IV236_002218"
     CDS             4269..5903
                     /locus_tag="IV236_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772392.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6066..>6264
                     /locus_tag="IV236_002219"
     CDS             6066..>6264
                     /locus_tag="IV236_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772393.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2188 a         1026 c         1273 g         1777 t
ORIGIN      
        1 tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt ttgtatttat
       61 tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga aaaaggcaga
      121 aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat catttgtaaa
      181 agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac caaataatca
      241 cgagacgatt cttactattc agaattttct tcaattactt caccgaagat ttggagtaga
      301 agatgaagtt gatattgata cttctgttta taatccttca agattatgca aattagttgg
      361 tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat ctagtttcct
      421 ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac agatagttga
      481 agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta gagaagtgtt
      541 accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat taacttatac
      601 cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat gtccattaag
      661 aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata ataagattcg
      721 atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg taaaaaaata
      781 tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc aagaatatta
      841 ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag gtcaagaaga
      901 aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac aaaaagtcat
      961 tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa tagaaggtgg
     1021 atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca ttaaagctag
     1081 aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc caatcttttt
     1141 tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc agttacaaga
     1201 ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc aattaacaac
     1261 taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag gaacgaactt
     1321 agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa acgttacgaa
     1381 ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa cgggtaaaac
     1441 cactacgtta cactttattg cctctttatt tggaaatcca actacggttc ttagaaatat
     1501 gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag gtgttccact
     1561 aatactagat gagttaggag cagcaactca aaataacttt agctcgttga tctatcaatt
     1621 agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc cacaagagac
     1681 tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt tcaatgagaa
     1741 attaactggt ttgaaagttc gatatatcga attttctgat atcaagttta caaaggatgc
     1801 gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg gtataaagtt
     1861 atttcttttg aaactctttg aacaagggat tacaaaaatt gaagcaactt ttgtcgaagc
     1921 tcaaaaaaaa ctatgggctg ttttacctga acattctttg aagcaacgca ttggtaataa
     1981 tctagctttg atttatagtg gagccctcct aattcaagat atactgggct atcctttacg
     2041 attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg tagatactag
     2101 tttaggaagt gagcaaacgg tatttgaccg agtgaaaaca ttgttggtga tgaatagcca
     2161 taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg gaaaagtatc
     2221 aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg aggacttgtt
     2281 aaggcgagaa tttcaagtaa aggatctaac ctatcttttt aggcaattgc ttgattcggg
     2341 taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc gtcaagttct
     2401 tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat tgtaatttag
     2461 aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta ttgatttagc
     2521 aaaacaactg attacttatc tgtatgatag cgagcaaacc caaacggaag taaatgaagc
     2581 ttgatctatg cttcatttac ttcattcaat caaacatgaa agaaaggaaa attctaatga
     2641 ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa catttttcaa
     2701 atcaagcctt ttttacaaaa ccaatttact tggatttcga aataagaggg gtatctgctg
     2761 gatctatcgg gggtactctt caagcgttga agaacgaagg atatttagaa aatcactttg
     2821 tgcaacgttc gttcaataga agagtcgtga aaaaatggta tttagttcat tcatagagag
     2881 gggaatcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa gaagcgaaaa
     2941 ttagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa tatgaacaaa
     3001 ttccccaacg attttcagac aaaggagaat caggggaaaa agcggagcgt ccagcttttc
     3061 gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat gtttggcgtt
     3121 atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg tgccaacaac
     3181 atcaggttca agtgtattcg atttctgaac ctcttcctgc ttctttctca cacgcgtcaa
     3241 cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga aacgtcatta
     3301 ttgagaatgt tcgtgccgga ctttcctata aaaagcaaca acggcaatat ttgtcttctg
     3361 cggtaccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagta cacgaagcaa
     3421 ggattgttcg ctatattttt caactttatt tgaccaaaaa atatggctac aaaaagcttt
     3481 gtcaacgatt gacgcaacaa aagcttttct tccgcgaacg tccttttcag ccctatcata
     3541 tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga tctttgggga
     3601 agtatctagg aacctttgaa ccgattctct cgaaagcaac ctttttacaa gcccaagaga
     3661 ttcgccaatc aaggtgtaca gctaaaaaag atacgtatct gtacttatta cgtcaaaaaa
     3721 ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat acaaagaaga
     3781 cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatt tttcttcggg
     3841 cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa tacgaaggcg
     3901 tctatcaagc cttgattgcc caattaacgc aacaattagc cgaaaagcga aaagatgaaa
     3961 aacgcaaagt aatccaacaa aagaaacaag aagcacaatg gattaaggca tttgaagagg
     4021 gcaattgttc actggaagaa ctcaagcaac aactgcaagc tcttcaaaag ccgatgtcta
     4081 cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg cttcaactaa
     4141 aagacctctc tagtcgacaa ttattatgtg actatgtttg tcggattgag gtaacaaagg
     4201 ataaaacagt ttgtgggatt tacttccgac aactgacaca aaatatttta gaaaggaacg
     4261 aatgatctat ggaaatgata caaaccaaac gagtcgcatt gtatattcgc gtgagtacac
     4321 aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga cgacagtttg
     4381 ccgaacggat gggctatgaa gtggtagaag tttatgccga tgaaggaatc agtgggaaat
     4441 ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac catcgttttg
     4501 atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat gtcttacaaa
     4561 cagtcgagga attattgcga tatgacgtag gattacattc gatctcggaa caatttgacg
     4621 tgaccacttc gacaggtaaa ttgatgttac aactccttgg gagtttcggt gaatttgaac
     4681 gcaaccaaat tgcggagaat gttcaaatgt caatgcaaag cttagtgaaa gataaaaagc
     4741 gttatgcagg aggccgacgt ttaggctatg tcagtggtat tgacacgaat ggtcaaaaac
     4801 aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca aaatatttag
     4861 gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat caaacagtca
     4921 aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa atttatgcag
     4981 ggtatttaga atatgcgcgc tacatcaatt gggaaaccaa acgacggaag gggaagaatc
     5041 cgagaccgat tttagtgaaa ggaacccatg aaccgattat ttcggaagaa gattatcaaa
     5101 aagtacaaga acgccttgct ttagaaggaa aacaacccaa atggaaccat acaggtgaaa
     5161 atgtattgac aggcttgtta cgttgtccag aatgtggcgc accgatggca gcgagtaatg
     5221 tcacaaatac cctaaaggat ggcacaaaga aacggattcg atattattct tgttcagtct
     5281 ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa caagcggaaa
     5341 catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt ccccgattgg
     5401 tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa gaactcgtgg
     5461 tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa gctgctttgg
     5521 aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg actgaaaaac
     5581 gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa ggagagccga
     5641 ttcaagtcaa agatgtccaa cggatattga ctaatttgga tcgttttctt gcccagagtg
     5701 agaagaagca aatcaaagcc ttgtatcgta cctttataga gaaaataacc tttgatccta
     5761 accataagga gatgttggaa attacgatga aattcacgca tgccgtggta caacaattga
     5821 atgagcaata tcaaatagcc gtatccaaaa caaaggatac ggctattttt gtgttaaaaa
     5881 caccctttac gttaacgatc tagtctaggt tatatcaaac aacgttagag gggcgctaac
     5941 gcttctctag cgatttgaaa tcgttagaga agtagttagg gaggtcctta tcataaatag
     6001 ggatatttat taatgaatat ttgaaatata attgtatata agaattaaaa agagtgggga
     6061 tagatatgaa aaagatgggt aagtgtattt tatgtgaaaa atacactgaa cttactaaag
     6121 aacatgtacc acctaaaaaa tcagggaata ctggaggtaa aaagaccaga actggtaatt
     6181 tagatgattt ccttaagagc gattttacaa aaggtgattt tccgaagggg attaaaagga
     6241 aacctcaagg aaatgtttat tata
//
LOCUS       DACDID010000135         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDID010000135 DACDID010000000
VERSION     DACDID010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.137"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV236_002220"
     CDS             725..811
                     /locus_tag="IV236_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8772394.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV236_002221"
     CDS             complement(932..1621)
                     /locus_tag="IV236_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772395.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV236_002222"
     CDS             complement(1628..1906)
                     /locus_tag="IV236_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772396.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV236_002223"
     CDS             complement(1866..2780)
                     /locus_tag="IV236_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8772397.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV236_002224"
     CDS             complement(2762..3142)
                     /locus_tag="IV236_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8772398.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV236_002225"
     CDS             complement(3592..3879)
                     /locus_tag="IV236_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8772399.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV236_002226"
     CDS             complement(3899..4123)
                     /locus_tag="IV236_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8772400.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV236_002227"
     CDS             complement(4763..5296)
                     /locus_tag="IV236_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8772401.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV236_002228"
     CDS             complement(5317..6159)
                     /locus_tag="IV236_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8772402.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDID010000136         6159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDID010000136 DACDID010000000
VERSION     DACDID010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.138"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IV236_002229"
     CDS             <1..382
                     /locus_tag="IV236_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8772403.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IV236_002230"
     CDS             complement(776..1783)
                     /locus_tag="IV236_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772404.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="IV236_002231"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="IV236_002231"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8772405.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="IV236_002232"
     CDS             3679..4002
                     /locus_tag="IV236_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8772406.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="IV236_002233"
     CDS             4013..5695
                     /locus_tag="IV236_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772407.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
BASE COUNT         1964 a          931 c         1242 g         2022 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaagg
//
LOCUS       DACDID010000137         6084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDID010000137 DACDID010000000
VERSION     DACDID010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.139"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(296..781)
                     /locus_tag="IV236_002234"
     CDS             complement(296..781)
                     /locus_tag="IV236_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772408.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(920..1135)
                     /locus_tag="IV236_002235"
     CDS             complement(920..1135)
                     /locus_tag="IV236_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8772409.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1359..2159
                     /locus_tag="IV236_002236"
     CDS             1359..2159
                     /locus_tag="IV236_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8772410.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2178..4046
                     /locus_tag="IV236_002237"
     CDS             2178..4046
                     /locus_tag="IV236_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8772411.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4039..4530
                     /locus_tag="IV236_002238"
     CDS             4039..4530
                     /locus_tag="IV236_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8772412.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4518..5072
                     /gene="srlA"
                     /locus_tag="IV236_002239"
     CDS             4518..5072
                     /gene="srlA"
                     /locus_tag="IV236_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8772413.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5090..>6084
                     /locus_tag="IV236_002240"
     CDS             5090..>6084
                     /locus_tag="IV236_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8772414.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2149 a          967 c         1219 g         1749 t
ORIGIN      
        1 aagaagaaaa tatgtcaaaa ggtaaaaaag taaattcttc tatccaaata tagaaaaatt
       61 gaatctagaa aaccgcccga caataaattt cattaactca gttgaccgaa aatgaagtag
      121 ataccaaaaa tttatcaaga gagcaagtag agaagtgagt atattcataa gagaaatagc
      181 tgtaacgaat tattaggtta atgcaaatgc ttatttatac tccgtataac tttctcagaa
      241 aaaatgatac caataatgac ttgctagtac tgtaaatccg tataatatca tctaatcaaa
      301 atggtgttcc atttgcatct gtcatcgtaa tcgatccttc gggtgacact cgataaaaac
      361 caacgcttcc agatcctcct tgggaacgga tttgtttgct ggaagctgtg aacaaataat
      421 ctcgatcaat tttttgataa aagctaataa caatatcgtc actttgtaat tcggaacggt
      481 gggcaataag aaatagaagt gctttgtcag agttttctaa tggaagagaa ggtacagtgc
      541 tttcttgcat ttcagatgtt gaagaaatgc taaacgactg aatacttgat tggctatgtt
      601 gcgaatctac attaccagta gaagtattag taatggaagt agtagaagta cttttagttg
      661 aaccaacctc agttgttgaa atggctgctg aagattcttt aacagaattc tttgttttat
      721 atccacaggc agaaaaaagt attacagttc ccaacaagcc tgcgtaaatc cctttatgca
      781 tgatagctcc tcctaaaata gattatcttt attcagtata tcacttaaac aaagaaaagc
      841 agatggttta aaatgaaaaa accttcccaa taaagggaag gttcatgaag ataataactt
      901 ttttaataat tatagctttc taatatcttt catcaacttt tctagtaatc attgggaaaa
      961 atttcaagca gtagatcgca caaataccta cgacgatata aatgatcttc gcaaaaatag
     1021 ttgttgaacc accagcaatc atagctacta aatcaagttc gaacaagcct accaacagcc
     1081 agttcaaccc tcccacaatc aataaagtta atgcaatagc atctaaagtt ttcattagaa
     1141 ttctcctttc ttaaatgata aaacaattat atatcaaaaa gagaaacatt gcttatataa
     1201 cgctcaggaa ttttagcaat ctatgcaaaa agatgaaaat tttttttgct cttccattag
     1261 tacacatttt ttagctgaag ctgtaaattt catgtcttaa ttttttttag tgagctttta
     1321 tactggattt gtaagaaaaa agaaggagag gatcacatat gtcggattgg ttggatattg
     1381 caggaaaagt agtaattgta actggaggtt cctcaggaat tggaagaagt atcgtcgaaa
     1441 atttgttgaa gcagaatgca caagtagcga attttgatgt aactgaatgc cgtatacagc
     1501 atgaaaacct actatcttta aaagtcgatg tgtcttctaa aacggacata aaagaaggga
     1561 tatataaagt aatgaaacat tttaagacga tcgatggtct agtcaataat gcaggaataa
     1621 atattccttc tcttctaata gatagaaatc atccaaaaag caagtatgaa ttatcagaac
     1681 aagtttttga taagatgatc gctgtcaatc aaaaaagtgt atatttgatg agccaagcag
     1741 ttggaagaat cttggtgcaa aagggatcag gagtgatcgt gaatctttct tctgaatcag
     1801 gattggaagg atcagaagga cagagctgtt atgctgctac aaaagcagcg atgaacagtt
     1861 ttactcgttc ttgggcaaaa gagttaggaa agaggaatgt tcgtgtggta ggtgttgcac
     1921 cagggatact ggaagaaacc ggtttgagaa cacaagaata tgaagaagct ctttcttata
     1981 cgagaggtat aagcgtcgaa cagctgagaa acggttacag tcgaacaagt acgatccctc
     2041 taggaaggag tggtaagctg caagaagtgg cggatctagt ctgctattat ctttcagaaa
     2101 gatcaagtta tatcactgga gtcacgacta atatttcagg aggtaaaaca agaggataaa
     2161 ggataggggg gacgcacgtg acgctagtaa acagatggta ccaattattg aaattactag
     2221 tcactcacaa gaaagtttca atccaggaag tgcaaaaaga aatcaaaacc agtaatcaaa
     2281 ccataaaaaa agatattggg gagttgaatg aagaaattgc cggtattgca aaaatcatcc
     2341 aaaaaaacaa acattttgaa cttgaaattt atgattttga tttttttgat gagatcatgc
     2401 aaggaaaatt gaaaacagcc agtgatttca attcggcatc caaaagaatc gcttatttac
     2461 taaataggct gatcgaaaca gaggatttct tacgtatgga tgatttgtct gaagaagtcg
     2521 gtgtcagcag aggaacagtg gtcaaggatc taaagacatt aaaagagatg atcaatgatt
     2581 ttgatgtaaa gattacaggc acaccaaata aaggactaag aataatagga gatgagctag
     2641 aattacgact acttttattt tattttgttt ctccctactt ttcagagacg aaaacagaac
     2701 agtttctaga agaaaaacaa gcaatcaaac attttcagaa gaagacgaat gctagtaaac
     2761 aagaaatcac ccttttaacg aaagttatcg gtatttcttt gaatagaatc tctagtaact
     2821 atctgttgaa gaagcccatc aatcgttaca gtgggtgttt tgaatatatc caagaattca
     2881 atgaactgat caatcagttg gaagaaatct atgaactaac tttgagccaa tatgaaaaag
     2941 attttatcag ttttcctttg aacatcatca acacaagtat aacgatgata aggaaagcta
     3001 actctgattt gcgcttatat ttcgacccca tgatgatgcg tattcgacga ttagatctca
     3061 ttgatctgga tgagtccttc ttatatcaag agatgaaaca gcacctctct ttattgataa
     3121 atcgggtaat ttttcgttat cgattgactg atttatttta tggtgagata gaaaaaaagt
     3181 atcctttttc ttaccaaatc gctagcgaat gcatacaagc attagaagct atcttgcaac
     3241 ataagatctc taaagtagag atcagctacc taacgttgta tttggagtta gcatttcgat
     3301 atcaaaaaga gcatttttcc tctaaaaaag tagccattgt tcatacgaca ggaaaaggaa
     3361 cagcattaat gatccaaaga caaatcaaaa ggatattggg gagggatata caaataacac
     3421 atgtttcaga agaacaatat gaaaaagtag atctaaacaa atatttcgct gtttttacaa
     3481 cgattccgtt aaaaaatgta aatgaagaga caccggtgat ccatttgact aatctttttg
     3541 atgactcttg gttaagggca caatggcaaa aagcaaaaaa aaagcgatta gccgggtttc
     3601 attctactgt ttttttctat caaatatgga aagaaaaaaa gacgtatcaa gaatatttaa
     3661 acaaattagt cgctcctttg atcagaggct attatgtgga tgaagaattt gaaaaaagga
     3721 tcttgaaaag agaatatcag caaagcacat ttgttaacaa agaatttgct tttcctcaca
     3781 caaccaacca gctatctcaa cacattgttt tatcagtagg gctaataact gatcaaggtt
     3841 tagcagaaac aggaggaaat ggagttattt ttttatttgc catacctgaa gaaatgacgg
     3901 ctgaagttga gaatgaatta tttgagctat acgatatcct atttgaactc ataaaaatgg
     3961 gaatagatga gtttattgaa ttagcaccga cacaagaatc atttttaaaa ctaatcaaaa
     4021 gtaaaggaga gagacttcat gaatgatatg tatctattca tagttttcgc agcttcagct
     4081 tatgtgctgc aagcagttat cagttggaag caactaaagc atttcaatca aacatatagc
     4141 gaactgcgaa gaaaaggaaa agtagcgatt ggtaaacgac ctgggaaaat ccgttcaggc
     4201 acgattgtta tgctagctat agacgatcat gatcaagttt tagaagtcag aaaaatgcaa
     4261 ggggttacat ctttggcaaa atttcattca ttacctgtct atgcgggtga agatatccat
     4321 tacatcgatc gttaccatcc ctcagttcga gcggaagatc gtttgacaca agcagcttta
     4381 gaaaatgcca gagaagtata cttacgaata gaggcaggaa atgaaaaaag tgaacaacct
     4441 tcattgcctt ttattgctat gctcaataat tttcggatat ggaaaagtac gatcaaagaa
     4501 aaaaggagtg acaaacgatg gatatcataa caaaatttgc tgagggattt atgaggctgt
     4561 tccaaacagg tgcagaaaca ttcataagct ggatgacagg aatcgtacca gttgtcttga
     4621 tgctgatggt tgcaatgaat acgctgattg cttttttagg tgaagaccga gtaacgaaag
     4681 tagccaaatt atcagcgaaa aatccgctta ctcgttacct tgtgttaccg ttcttatctg
     4741 cttttatgtt gggaaatcct atgtcattta caatggctcg tttccttcca gaatactaca
     4801 aaccaagcta ttatgctgct caagctcaat tttgtcatac gagcaatggt gtattccccc
     4861 atattaatcc tggagaattg tttgtatgga tggggattgc tcaagggatc gaaaccttag
     4921 ggctaaattc gatggagctg gctatccgat acttgcttgt gggactgctg atgaacttta
     4981 ttggtggttg gataactgat tttactactg gatttgtatg cagacagcaa ggaatcgttc
     5041 ttagcaaaaa agtagagctt tcagttgatt agagaaagga gagttttaaa tgtatggtga
     5101 tattaagatc gtaagaggaa acggcgggta tggaggtcct ttgattatta ggccgacaaa
     5161 agataaacac aagttcatct atgtcacagg tggaggtgag aggccaatga tcgtagataa
     5221 aatagaagaa ctgacaggta tggagtctat caatgggttc aaaacatcaa tacctgatag
     5281 tgaaatcgcg cttgcaatcg ttgactgtgg agggactctt agatgtggaa tttatcctaa
     5341 aaaagggata ccaaccatca atatcgttcc cacaggaaaa agtggcccat tagcacaata
     5401 tatcactgaa gatatttatg tttcagcagt agggttagaa caaatacagc cactaatgga
     5461 aactgatgat aacaaagaaa cagtaaatac tgcatctcca gataaaaagt acaaatatga
     5521 tacttctaaa aagataaccg agcaaaaagc agagcagcaa aactggatag caaaaattgg
     5581 aatgggcgcg ggaaaagtag ttgctacatt caatcaatca gcaagagaag cagtagatac
     5641 gatgatccgg acagtgatcc cttttatggc tttcgtttcc cttttgattg gtgtgataca
     5701 aggttctggg atcggtaatg tatttgctaa cattatggca cctttagcag gaaatgcagt
     5761 tggattaaca atcattggct ttatttgttc actgccattt ttatctcctt tgcttggtcc
     5821 aggggcagtt attagccaag tgattggaac attgatcggt gtagagattg ggaaaggaaa
     5881 tattcctccc caattagcat taccggcact atttgctatc aatactcaaa atgcatgtga
     5941 ttttattcca gtcggtctag gtttggaaga agcaaaagct gagacagtag aagtaggtgt
     6001 accatcagtt ctttattcaa gatttcttaa cggcatccca cgagttatca ttgcttgggt
     6061 agcaagtttt ggattatata gctg
//
LOCUS       DACDID010000138         6067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDID010000138 DACDID010000000
VERSION     DACDID010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.140"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            182..421
                     /locus_tag="IV236_002241"
     CDS             182..421
                     /locus_tag="IV236_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772415.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(783..1043)
                     /locus_tag="IV236_002242"
     CDS             complement(783..1043)
                     /locus_tag="IV236_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772416.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1228..1725)
                     /locus_tag="IV236_002243"
     CDS             complement(1228..1725)
                     /locus_tag="IV236_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8772417.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1855..2565)
                     /gene="budA"
                     /locus_tag="IV236_002244"
     CDS             complement(1855..2565)
                     /gene="budA"
                     /locus_tag="IV236_002244"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8772418.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2578..4242)
                     /gene="alsS"
                     /locus_tag="IV236_002245"
     CDS             complement(2578..4242)
                     /gene="alsS"
                     /locus_tag="IV236_002245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8772419.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4450..5112)
                     /locus_tag="IV236_002246"
     CDS             complement(4450..5112)
                     /locus_tag="IV236_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8772420.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5122..5937)
                     /locus_tag="IV236_002247"
     CDS             complement(5122..5937)
                     /locus_tag="IV236_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8772421.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
BASE COUNT         1916 a         1257 c          938 g         1956 t
ORIGIN      
        1 aggcataatc gttaatacag atggtaacca atgtatctgg tttgaactgg gatatcaccc
       61 tctccatata tgtattacta agactaatag atactataaa agacttgata gttttgcatt
      121 gattttgcct aatactgagc ataggtgtat gctaaacgat actataagga gtgtgtacat
      181 aatgaaaaga attctgttta tcagtttatc aaatttattg ttacttactg cttgtgggac
      241 ttctgcatca aagaacatac cttcagatac gcaaatttcg aatagtatcg attcaacaat
      301 ttctacaaat gagaaaaatt ctccggtaga aacttccgat ataaaagttt ctacctctgc
      361 aaaaaacagg gtaaagaaac tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta
      421 gcttcttcat cttcttcagc aaataatgat gatagtttcg cgtttactcc taactaaatg
      481 caacctttac aaatgcttta cgataacaag cctgagctaa atgatcctga tatcagtttt
      541 gcattttttg aaatgattgg atcggatgat ctttttaaaa ctactagttt gtctatccga
      601 aaataggaca gttctggcag tataggtttt tatcgagttt ctccaccagg cctcgttaca
      661 gagacagatt ctctaggcga accttttgaa taaatcaaaa atcatgaaca atcactgcca
      721 actaaataaa gtgccttcaa aagcttggtc ttctcgcttt tgaaggcact ttatttaaat
      781 ctttatgaaa ctaaagtatg ttcatatttg ttgctatagt tatatagata ttctgttgtt
      841 tccaactcat aaacgtgagt atattttgcc actacttttc cgcatttttc taatatttca
      901 ttgcgatcga cgtcatgata ttcgtctacg cagatcacaa cacaattttc taatttactg
      961 attacttctc caaccacttt ttgtttcaag ccaataggtt ggcaaatata tttttttcct
     1021 aaaatgatag tttcgttatt catatttcat ccatccttaa tgttttttca ttattcaaaa
     1081 tgataatacc attaaaatat gattgtgaaa atcaataaca cttgtgattt tataaaaaaa
     1141 taaaaaaacc gaaaaacaga aatgaccact gtctttccgg ctctttttat tcttgatttc
     1201 tcttcttttt tgtttcaata gacgatttta gttattaata aatgaaaaag catttatatt
     1261 aaaatttgct aataaaagat ctacatattt ttctagaaat gcttgatcga tttcatcata
     1321 ccctttcgtt actccgcaat cgatatctaa aacaccgatt aaattacctt cttttaccat
     1381 tggcactacg atttctgatt gtgcggctga atcgcaggaa atgtaatttg catgggtttt
     1441 gacatcatca acgatcaatg tttcttgttt ttcagctgat tctccacaaa ctccttttcc
     1501 catcttgata cgtgtgcaag aaactcgccc ttggaacggt cctaaaatca attcattacc
     1561 atcgaataaa taatatcctg caaagacagt ctcaggcaaa gtctcattca acaatgcaga
     1621 tgaatttgct aaatttgcta accagttatc ttctgcttct aataatcctt gttgctgtaa
     1681 taataaaatt tcgtatgcat cttttttctg tttttctgtc cacatgataa aaaccgctcc
     1741 tttttgatct atggagaaga ttatagctca gtttttttgt aaacgctagg tttcctcctc
     1801 aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat ctcgctgtct ttttctattc
     1861 tgcttgttgg atttctgctg ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg
     1921 atcatcaact ggaaaatgct gtacaaggga cgagatgcgg ctaatctcaa cttttccttc
     1981 agccacttca aaatccatta catgtccgcc aaatgtatgt gtatcatcga taaaatgcag
     2041 gtgaaatccc gctgcagcaa ctccttggaa taattcaggc gtaaagaacc cgactaatgt
     2101 tccttggata ttgctttgtt ggaattctgg ttgagactct gaaatttttg ctagtcttct
     2161 gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata tttttgaata atccagttat
     2221 cttcactgcc aaaaataggt tttccccggc ttctcttact aatatgtctt ttttcaattg
     2281 ttctccatct gcagtttttg agacagaaaa actagtatct ggcgtaaaat ctgtaattgc
     2341 tgcatagggg gtaagttcac ttccatctaa ttgggcaact gagccatctg atcttccttg
     2401 ataagctacg ccatctaaaa agatgacttc accatcaagc ccatgcaatg ttcccaatcc
     2461 cagagttcct ttttctaata tatgagcaat cgtttctgtt ccatccatca agccggccat
     2521 caaagctcct aatgtcccgt gctggtataa gattttttca gtcatgtcga cacttcctta
     2581 atagaattga tctggtaaga ttgtttctcc caatttctca ttatcacggt aatcaatcgg
     2641 aatatcaata attacgggtc cttctgtcgc aaatccttct tctaaagctt tttctaattc
     2701 ctctacactt gtagctcgga ttcctttagc gccaaaagct tctgcatatt tcacaaaatc
     2761 aactggacca aagtctacac ctgacgcccg ctgatatttc attttttctt ggaactctac
     2821 catattgtag tgtccatcat tccaaatcaa atgaacgata ttcaattttt tacgtaccgc
     2881 cgtttctaaa tcctgggcag agaataaaaa tcccccatcg ccggaaactg atacgatttg
     2941 tgtatttgga cgcaccaatg cagcagaaat cgcccatggc aatgcaacac caagcgtttg
     3001 cataccatta ctgaataaca aatgacgagg ttcataactt ctaaagtggc gtgccatcca
     3061 aatataatga ctacctacat ctacggtaac agtcatatca tctgttactt ttgactgaag
     3121 cgtgttaata acttctaaag gatgcaagat acctgcttct ccttttgatt gaacaatgtc
     3181 ccgctccgtt aattttgctt gaagagatgc taaatattct tttgcatctt ctgacacttg
     3241 ttgaggctct aatgaacctg tcaataaatc aagagtggct gagatatcac cgatcaattc
     3301 tctttcaggc tgcataaaag gatcgatttc agctggcgct tcatcgatca caatgatccg
     3361 cgcatctttt tccgcattcc agtttcttgc ttcatattcg attggatcat agccaattgc
     3421 aataactaga tcacttcgtt tcaaaagcat atctcctggt tggtttctaa ataaacccac
     3481 acgaccaaag aaatgcgctt ctaattcacg tgagatcact ccagctgctt ggaatgtctc
     3541 tacaacaggc aatgctgtct tttcaactaa ttgcctgatg gctaacgttt ctttttcact
     3601 tgatcctcgc atacctacta atagtaccgg tagttttgct tctctaatgc gttcagctag
     3661 gtaacggata tcatctgcag aagcagaacc taatttagga tcgctcaatg gcttgatgac
     3721 attaccttgg acaggtgcat ccactacatc ttgcgggata ctgataaagc tcgcgccttt
     3781 ttttgaactc ttagccatac gataagcatt ggcaatcact tctgaaattg tttctggatc
     3841 ttggatttct gcactgtatt ttgtgattgg ctggaacagt gctgcattat ccatgctttg
     3901 atgggtcaac ttcaatagat cgcttcgttt tacttgtccg gcaattgcta aaactgggtc
     3961 accttctgct gtcgctgtta ccaaacctgt agctaagtta ctagcaccag gaccacttgt
     4021 tgcgataact actcctggct ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc
     4081 attttgttcg tgacgagtca cgattaattc aggaccttga tcctctaatt cattaaatac
     4141 accatcgatt tttgcacctg gtataccgaa tacataatca acatgatgat taatcaaact
     4201 ttctactaca gatttcgaac cttgtttcat tttttcagcc attgaacatc gtccttttta
     4261 tgctttattt ttaattatta gttagaaata atcacaaata agtgatacag tttctaaacc
     4321 tgtttcatac aaaagtatag cacgttttat ttcatttgtt tagccatatg tttcaaaaaa
     4381 tgaaaaagaa tgacagtttt tacataaaaa aaactgtcat tcttaattga aagtttcaat
     4441 ttataaagtc tacagatcca tagttctcat caatttacga ttgttgaagt aaatcaaacc
     4501 aaatacgata gaaggaccta gtagaataca ccattggggc tttacgaaaa agaagagaaa
     4561 caaacttgtc aatagtaaaa aatctagtaa actcacatac cattttttca acgtatagta
     4621 gaacgctatc cgtagaactt gatttggttt cgacgctgga tttctcacct gaaaatacca
     4681 gcagttggtg ctaaaggaaa ctgataaaca agcaagtaaa acaaacaacg gaatcaacca
     4741 ttttccataa gaaaaacgta taaaaaagaa acaatcaaaa atcattatca cactaactat
     4801 ccaagcaaat agccaataaa aaaatccctt tagccaaaat tggcgaaaca tctttatgaa
     4861 caaacgtact ggtgaaaaac tattttcttt tgctaattct tctgtcatac ctatcatcac
     4921 gatgcaacct ggaccaataa aaagtaaaga aaaaataaag gaaaagacat tccttccatc
     4981 tatcgacaaa aggaacgcag ctgctatata caagatattg acacaccaaa aacaaaggct
     5041 acttgccaat gattgataca accaataaaa gaattccata taaacatttt tgtcaaaacg
     5101 ctgctctttc ataatcacct cctattcctt cattcctgat gaagcgatcc cttctacaaa
     5161 atatttttgc atcgctaaaa atataaaagc aacaggaaga acagaaacaa ctgaggcagc
     5221 catgattaat gcgtactctg catcaaatag cccaataaac attttcaacc ctaactggat
     5281 tgttttattt tccgtagaag acaaataaat gaatggcccc atataatcgt tccatgtatt
     5341 aacgaatgtg atgatcgtca aactggcaat cgccggtttt gtaagaggta aaatgatctt
     5401 gcgatagatt ccccactctg ataagccatc gatccgcgca ctctcacata aagaatctgg
     5461 gatactactg taatattgct tcaacaagaa cacaccaaaa gcattgaacg cttgtaatag
     5521 caccaacgac cacaacgtat ctgttaaacc cacttttctc atcataatga actgcgggat
     5581 catataagac tgccaaggga cagcaatcgt gccgatatat gctaaaaata aaatatcgcg
     5641 ccctttaaaa tttagtttag aaaacccgta agcagcaaaa ctagaagtaa acaactgaat
     5701 caacgtgatc acaacgctta aaaaagctgt attcttaaaa aatgtcacta aaggaatacg
     5761 agaccaaata accagataat tctctaaatg ccaagtttta gggatccatt gaatcggaat
     5821 cgtgaacact tcattatttg ctttgaatga agcagaaacc atccataaaa aaggaatgat
     5881 cgtcaataaa gctaatacaa tcagcagaat catacccaac cattgaataa agggcatgtt
     5941 ttttgaacga atttttgctt tttttctttc atttcgaaca cctccaaagt tataatcttc
     6001 atttctttgt aatgattaca atccagaaca acggttatta tagctgattc atttccgttc
     6061 ttcttat
//
LOCUS       DACDID010000139         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDID010000139 DACDID010000000
VERSION     DACDID010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.141"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV236_002248"
     CDS             <1..395
                     /locus_tag="IV236_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772422.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IV236_002249"
     CDS             398..1228
                     /locus_tag="IV236_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8772423.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IV236_002250"
     CDS             1244..2095
                     /locus_tag="IV236_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8772424.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IV236_002251"
     CDS             2092..2451
                     /locus_tag="IV236_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8772425.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IV236_002252"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IV236_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IV236_002253"
     CDS             2624..2929
                     /locus_tag="IV236_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772426.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IV236_002254"
     CDS             2929..3240
                     /locus_tag="IV236_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772427.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IV236_002255"
     CDS             3407..3823
                     /locus_tag="IV236_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8772428.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IV236_002256"
     CDS             3823..4491
                     /locus_tag="IV236_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8772429.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IV236_002257"
     CDS             4488..4865
                     /locus_tag="IV236_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772430.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IV236_002258"
     CDS             4862..5161
                     /locus_tag="IV236_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772431.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IV236_002259"
     CDS             5403..5819
                     /locus_tag="IV236_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8772432.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDID010000140         5945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDID010000140 DACDID010000000
VERSION     DACDID010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.145"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..633)
                     /locus_tag="IV236_002260"
     CDS             complement(70..633)
                     /locus_tag="IV236_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8772433.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(648..1691)
                     /locus_tag="IV236_002261"
     CDS             complement(648..1691)
                     /locus_tag="IV236_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772434.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1824..2228)
                     /locus_tag="IV236_002262"
     CDS             complement(1824..2228)
                     /locus_tag="IV236_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8772435.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2461..3096)
                     /locus_tag="IV236_002263"
     CDS             complement(2461..3096)
                     /locus_tag="IV236_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8772436.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3362..4414)
                     /locus_tag="IV236_002264"
     CDS             complement(3362..4414)
                     /locus_tag="IV236_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772437.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4427..5365)
                     /locus_tag="IV236_002265"
     CDS             complement(4427..5365)
                     /locus_tag="IV236_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8772438.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1871 a         1253 c          951 g         1870 t
ORIGIN      
        1 aaattccata aaatataaat aatagaccta gcatcatctg gtataaaatg gatgctaggt
       61 ccgttatatt tactcaaaat tgattctacg aatagatctt cttatcattt ctattttagt
      121 agtcatgcgt tgttgttctt ctaaaggtgg taatggtata agtagtttag ataaatttgt
      181 accatttaca tttggttgcc cagttccgta agaaattttt tctaataact tccaatataa
      241 tggcgagtct aagaagcagt ctacgtattc tgataataat ttctcaacta acctaattcg
      301 aatcaagtaa gaagcaaaaa cagattcctc acttatttcc ttaaccaaga aactttttcc
      361 catagttcct cctgtccttg ctatcaaaat gtcattttct tccaatctta aatcaactag
      421 tttagaatta ctaatatcac agtaaggcac actggaccaa tttacacgtc cctcttgtat
      481 atctgtgatt ctcaaaaact taacattgcc ttgttttttc gccgaatctg tatagccata
      541 ttgaatctta gtagaaatac ttcctaaata aacccatagt cgaacatcgc tagatgattt
      601 ttcttgtcgt tctttatgaa caacagtgat catcttgagt tgtccgacta ttttttagtt
      661 gcagcaatct ttttgggacg cttatttgca tgaagcaacg ttgctaagtc tagtaataaa
      721 acattggcta acacaggatt ggctttctcg cgatacgctg cggcgccact ttcaaacaat
      781 actgctagac gatcttctac aacaacgatt tgttccaaat acggtggaaa tttgattcgt
      841 tttaggatcc ggctaggaga gtcccgctcg gacactaaga gttccgaggt ataaggacca
      901 taagaacgac tggctaagag ataatggcca taaaaagcca caccttgaat cttatcagat
      961 gtttttctgc tcattgtcac ggttggttgg attttgtctt tgcggcgaat tgctgttttc
     1021 atttcagcta tcgtcggcaa tagatagaaa gtcaactgtc cttcaccttt tagtgtgaag
     1081 ttgccagata ccagttgatc attgttttga gtcaaatacg atgcttgtgg aatctctgag
     1141 agatcagttg tcagccaata agcgattggc tgtcccgtct cttcagatgt atctgctaag
     1201 atatcgatca aacgtaaagc ggctactcgt ccagtagcag atcctgtgat tgtcaaccat
     1261 aaaagttccc gttttgtatc atatactaag ccccctacat gtggccgttt aggtaaaatc
     1321 agcagtttga tcggctgtcc tgtttctcta tccagtacat gaataataga atgatgctga
     1381 tggtcatagc agtaggcaga aataaacaca aaatcttgag aaaaagccaa cccttgcggc
     1441 gtcatccatt gacaaacctc attttcgccg gtttccggca ccaccattct tgtttctgtt
     1501 aatccaggaa tgttatattg ttttgcttca gccttttccc aagctgcttg aacaatagga
     1561 taccgtttta actttttttc aatatcctga tgtatccgtt gctgcttcca atgcttaaac
     1621 tttaggacat agtaaacaag aaggccaatg accagcaaca ctaaaatcat tattgctacc
     1681 cgctgtgtca tttttttgcc tccttgttct attcgatcaa tcgtccatat accgctatta
     1741 attaccgtaa ctacatgttt cttttactag cgtttcttta taggatcttt cataatctca
     1801 tttatagtaa cattaagaat cccttattct actatagtca cttccacatc aatattgcct
     1861 cttgttgctt tagagtatgg gcaaaatgcg tgggcttctt ctgcgatttt ctctgcttgg
     1921 tccttctcaa gtcctggcaa agaaacttct aaaacgaccc cgatttggta gcccccatct
     1981 tctaattgaa ataaagatac ccgtgctttg acttcagaat cagactcaag attattttct
     2041 tccagcatgt gttgaaccgc gccgttgaag caagatgcgt atccagccgc aaaaagttgt
     2101 tccggatttg tcccttcact atggattcct gggcggtcga tcttcatgtg catacttccg
     2161 tttggtgcct caacttcgcc atcacggcct ccacgattga tcattgctgt ttcgtacatt
     2221 tttttcattg agatcatcct ttctttcgtc gttctatatt aagtgtaacg agtatttttt
     2281 aatctgacaa aggtaatgct tgcattcggc gacatcagtt agacaacatc gatgatcagt
     2341 gatagaagca gtttatctat acaaaaaaag aagggttcta tgatatttgg tgtccttact
     2401 cgccttgagt aacaacacca aatatcatag aaccacttaa tcagcttcac actgcttttt
     2461 ctactttcgt agttgaaaaa aacttaaaca aatcatcggc aaatagttaa agaaaaaact
     2521 taatcgttga aagaagccca attgcttaaa gaaggcaaac tgactaatcg acggaatccg
     2581 tgctaatccg taaagacctg caattagtaa actaataact gttaaaacaa gataaaaatg
     2641 gctaagcgta ccttgaccac tttgacgata aagcagaaca agtaacaatg ggaaaagtag
     2701 aaagcctgca tagcctagcc cagaacctat attatggatc caagtcgata gattacaaga
     2761 gctttcattc gtattgatac tgaataagcc agtaaaaata caatcaccta tcccatataa
     2821 accaactgtt gcaacaacaa gtattgcaag tgtttttgat gttccaacaa ataggtgata
     2881 caacgcaggc aacgaaagaa caaaaaaaag tccagataca actgaccaga ccaagaaagc
     2941 acgtctgact ggactatcta cgtcaccaaa cacactaatt acggtagtca tttgattcag
     3001 tttgggataa aaaaggccaa ggatatacgg tgtcgataaa tcactcatca cacctaataa
     3061 taaaaaataa aaaccatatt tttgcaaaaa aatcattttc ttcccctact ttttagtgat
     3121 tattcgatac atccaacaat caaatttttt ctcattttaa cacatatttt tattctagtt
     3181 gtcttttgaa caatcaataa cataaaggtg agtttcttct acattttaaa aaatcgaatg
     3241 gcactgaaga gttcagttgt cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag
     3301 tcagatgttg ttgacatctc acttacgcat ccattgattg aaaatttctt agtaaaatct
     3361 actagcctaa aaatttgaag acttcttctc gatcatacag tttaaatact ttctcgcata
     3421 aaaattgagt aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa
     3481 agccatttag ccctgcatct aaagaagcca acggaccaac aagtccgacc acgccgaaac
     3541 cagcagaagc aggcgtacct gaaatagaaa atagagcgac aggaatagct gtaatcactg
     3601 cactaactaa acaaggaata agaatcacag gttttctaaa tagattaggc atcatcatct
     3661 tcatagcgcc taaagcaatc gcaattgtca cacctgactt attaaccttc caagagttca
     3721 ctactaaaac aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca gaaacaccat
     3781 ctagttggat tgccattccg attgctactg ttgaaatagg agagataatc aaaaaggcaa
     3841 aggaacaacc aatcaagata gacattaaca gcggttgtaa ggtagtgaag ttattaatca
     3901 atgatccaat tgcagaagta attgctgtta caaaagggaa taaataaaaa ccaagtaaac
     3961 ctgcacctac acctacaaca atcggtgtta acacaatcga cactgaccca aaactatttc
     4021 caatcagtaa aatcagcccg acagcaatcg atgctgtcag catcgtatta ataatatcgc
     4081 ctgtaccagc accaacgtaa acacccgttt cacccaaatc aggtgtgaat ttgactacac
     4141 cagaaccaac aaaagaagct ccggctacaa ccatcattct catcggatca aatccaaatt
     4201 gtaaggcaat caaaccacca atgatcaaag gtgttgctag ttgaaaaata actgctaact
     4261 gtgcgatcgt taccgcaaag ggatacgcac taaaatattt taaaatggca cctaatactg
     4321 cattaggaat caatccaata atgattcctg ttgctgtacc agcgaggact ttattcagaa
     4381 aaattttcgg tgatattttt tgtgaagttt ccattcaatc tctcctttat tttacgttca
     4441 aactatcttc ttttgcatgg acaagctcag tcaagaaatc acaataaggc gtcgcaacac
     4501 catatttctt cccttttcta gaaattgcgc cattgatata atcgatttct gttaatcgat
     4561 ggttcttaat caagtcttga tacattgaag gataatgtaa cccaattgtt tccgggtcaa
     4621 aacaagattc acaatgtgca atgacttctg ggacatctag ttcaatcttc tctactgctg
     4681 ctactttggc aaactcattg acaatcgtcg ccaccatttt atgagcagtc gatgtttttc
     4741 ctagctcagc catattaacg tctaagattg tgcatagtcc gttcattgtt ccgttcacac
     4801 atgctttgcg ataaatagaa tagtgaatat tgtcagaaaa atgagcattt aatcctgatt
     4861 cagacaattt gtctgctaat ttttttgcag ctgcttcttt tccatcacct aaattttgta
     4921 attctactga accacttcca aataatttga cctgaccagg accttcaaga cctgccgtcc
     4981 acatggtatt tccaatatag atattttcca ttggtacaaa tttctcaata atatcttcat
     5041 gcccgatacc atttaataga cataagacct ctgtatcttt tttgattaat gattggatat
     5101 cctgcagcat tttttccagc tgcatcgctt tggtaaatag aataatcaga tcaacttgat
     5161 cttctttttc tacttcggat tgaagaacga ttggtagttt tgcttctacc tctttaccat
     5221 taaaatttgc ttgtaatcca tgttctttga tttgttggac atgttctgcc cagccatcga
     5281 ttaataatac ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt cccattgcgc
     5341 cagcgcctgc aattgctatt ttcattgatt cttcctcctt tttctttcta ttatactcat
     5401 ttgagaatct tttcacaacc tcgttttgtt attcttctct tatttatcat gtttatatcc
     5461 accattcaag ttattttatc agcaaatcaa taaaaactgg aatagaatcc caatttcatc
     5521 gtaagagaaa ctagactagc aataagaaaa tagaaacatt catagaaaaa atctcactcc
     5581 tctgattata aaagaggggg cattaacctc gtgtatacta aaaccttcca ttcattcgat
     5641 ggatgatttt ctttttgtga aaaaatattc attgtagtac gatgttgcgc aacacagacg
     5701 gatggtattg cttattattt attaaaatcg aacaatacac ttatttaaaa acttctttcc
     5761 cagattttct tccattttta cagttataac tagtattatc atgaaaaata gccttttatt
     5821 gtctatggga ataataattt aaaattggta cttttttatt aattagttca tcgcccaata
     5881 atctctccag tttcttagca tctcctgact caaccggaat tatccaagcc cagtctaaat
     5941 aattt
//
LOCUS       DACDID010000141         5905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDID010000141 DACDID010000000
VERSION     DACDID010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.146"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            182..1078
                     /locus_tag="IV236_002266"
     CDS             182..1078
                     /locus_tag="IV236_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8772439.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1228..2169)
                     /locus_tag="IV236_002267"
     CDS             complement(1228..2169)
                     /locus_tag="IV236_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8772440.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2295..3416
                     /locus_tag="IV236_002268"
     CDS             2295..3416
                     /locus_tag="IV236_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8772441.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3674..5176
                     /locus_tag="IV236_002269"
     CDS             3674..5176
                     /locus_tag="IV236_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8772442.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5243..5770
                     /locus_tag="IV236_002270"
     CDS             5243..5770
                     /locus_tag="IV236_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8772443.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1943 a         1038 c         1230 g         1694 t
ORIGIN      
        1 tcagacaatt acaactattc agaaaacttt taggcgaatt ttaatagaag aatgatttat
       61 ttcatcatat tttcatacaa atgttaaatt ttcatacaaa tataggaaac gttttactgg
      121 ctttttacta tttaaacagg ctatcatagg aaaaagtgac agagaaagtg aggcggaaaa
      181 gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa tcactttatt ttttgccttc
      241 catccgctct ttagatgttt ggtccggaaa agatttacta cagtcggaat tcattaaatc
      301 agctagacaa cagttggcaa attatcagct gatcgttttt ttagattatg gatttgatat
      361 ggagatggct cgacgcgtgc gcccatttac caatgcaaaa attgtgttat ttttttggaa
      421 tcacttcaaa gcagaacacg aggcgatgct gaagcaggca caagatgaac cggcaatcga
      481 tgagatttat cattttgatt ttttggaagc aaaagaactt gggctgaaac ataatagttc
      541 attttattcg aaacatatga atttttcttc tagcgaacca aagacggatc tattttttgg
      601 tgcaacgaat aatgggagaa aagaacgagc agaaagctat aagcaggaat ttataaaaag
      661 agatgttacg accaactatt tcattcttcc acatcgaggg aatgaacaac ctggatatct
      721 tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc gggattctgg aactgttgcg
      781 ggagggtcag caaggagtga ctttgcggac atttgaatcc atgtattttc aaaagaaact
      841 agtgacggat aatcaagcga ttgctcatta tcatttttat cgtcctgaaa atattttttt
      901 gcttcgagaa cgtgatttag atgaattacc ggaatttctg catacaccat ttaagccaat
      961 cctatcagag ctattggaat tttatgatgc agaaaattgg gcaaaacgat ttatcactaa
     1021 cgattccact atttatgagc aatacgaata tgacctctcc tctgttgaaa acaaataaaa
     1081 aacctacatt ttgaagagat tatgtactga aacaaacaag cacaattctt atggcatcat
     1141 gagagttgtg ttttcttttt gatcttttat tgggcttaaa aaaagagaac cagctgctga
     1201 ttcagctgat tctcatcaga gagcctttta ttctgcgcca aggattcctt cttttgcatg
     1261 gactaattgg gtcaaaaagg cacaatatgg tgtagcaata tcatattttt gtccttttcg
     1321 ccaaatggca ccattgatgt agtcgatttc agtcaaacga tggtttttga tcaggtcttg
     1381 gtacatagat gggtaatgca acccgatgtt gtcaggatca taagtagaag caatatgttg
     1441 gtacacttct tcttgatcta gtatgattcc ttcttttgct gcgatagaag caaattcaga
     1501 tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg cctagttcag caatattaca
     1561 atccaaaatc gtgcataaac cattcaatgt tccatttaca caagcttttc gccaaatgga
     1621 ataacgaacg ttatggctat agatcggatt taaatttgct tcttgaaaga cttctacaac
     1681 tttttttgta aatgcttcgc cttctggttc caagttctct agttcgattt ctccatcacc
     1741 taacaatgtg acttttccgg gaccagctag accagctgtc cacatcgtga tcccaaataa
     1801 gatatttttc tttggcacat atttttccag tacatcttca tgacctaatc catttagtaa
     1861 acacaaaaca taagtattgt cagtaataat ggattggatc gaacaaaaca tatcatctaa
     1921 ttgattggct tttgttaatg ctacgatcaa gtccacgtgt tcgttgcttt cgatgatttc
     1981 ttctggtgaa tagattggca gttttgcgac cacttcttta ccgttaaagt cagcaatcaa
     2041 cccatttgta cgaatggctt caatgtgagc aggccaacga tcgatcaaga ggacctcatt
     2101 tccgctttga tgtagcatca atccgattcg gctgcccatt gctccggccc cagctattgc
     2161 gattttcatg ttttttctct cctttattca atgagttccc ttttagtata tagaaaaatg
     2221 gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa
     2281 agtgagggat acagatggat attttggcgg caattgattc atttaaagga agtgcttctt
     2341 catttgaact aaatcaggca gttctaaatg gctgggaaga tcgtaaagga agaaaaatca
     2401 atgtgccgat tgcagatggc ggagaaaata ctgcagaagc aatctattac gcgctcaggg
     2461 gaacatggcg taccattgat gggatggatc tcttattccg tcagcgaaaa atcagctatt
     2521 tattgacaac ttggcaagga aaaaacaccg cagtcatcga atctgcacaa gcattggggt
     2581 tagatctact tgataaacca acggatcaaa ctatccgact tgcttccagt tatgccttag
     2641 gagaacttat taaagatgca ctgcttcaag aagtagaaca aattattgtc actttaggag
     2701 gaagcgggtg tagcgatggt ggtctaggat tactccaatc cttgggtgct tctttagaag
     2761 ggtacacaga aggaaatcct ttattatcta ctaaaaaact tcattttgat cgaagaaacg
     2821 ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt taccaatcca tatacaggtc
     2881 aacaaggagc tgcttggcaa tttggaagac aaaaaggagg aatagatacg acgctagctt
     2941 tcttagataa acaagcagga aagattgctc agcaaataaa agatttctgg gaggtggatt
     3001 tagatgatat tccaggaagt ggagcagcag gcggtattgg tggagccctt tttttgttgg
     3061 gagcgaagat ggtttctggt ttcgatttag tcagccaaat cactggattg gaaaaaaaga
     3121 tcgaacaagc agatgtcatc attacaggag aagggcggat cgatcagcaa acgatccacg
     3181 gaaaagttcc atacggtgta gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta
     3241 tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca attgtcttaa
     3301 gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa
     3361 aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg aagtagttga
     3421 caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg
     3481 cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag
     3541 gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg
     3601 tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac
     3661 gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca
     3721 aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact
     3781 tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc
     3841 ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga
     3901 taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat
     3961 cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac
     4021 actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca
     4081 tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc
     4141 ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat
     4201 cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac tttatcaagg
     4261 gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa
     4321 tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg
     4381 gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg atactgaatc
     4441 tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga aacgatccaa
     4501 agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat taaaagatat
     4561 cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc gacttttaag
     4621 cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc attttacaat
     4681 cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt catccattat
     4741 ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc agccaaaaca
     4801 tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt tggcggaatt
     4861 tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa agcttgggat
     4921 ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac ggaaaaaagt
     4981 ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg aaccattgaa
     5041 ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg tgaaacctgc
     5101 catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg agattatttc
     5161 tcttgagaga atctaaccct tgtaatctca agaacaacaa ggtatggtta aggggaactt
     5221 tgccaacgaa ggagagaatc acatgcttta tgtaacgaga cacggagaaa caacttggaa
     5281 cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag gacagatgca
     5341 ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga aaatcatcca
     5401 ttcccctctt caacgagcaa gagatacagc acaagcagtt gctgatcgtc tcagcttgcc
     5461 gctgaccgtt gatgaacgat tggtagaaat ggattttggg gattatgatg gactgccaag
     5521 caaagatgag aactttcaaa aagcacgttt agcgttcgct gtacgattcc caaatgggga
     5581 gtcagtctta gatgtgtatg cacggatcgt tcctttattg aaagaatgca tcgaagacga
     5641 agaaaatgtc tatctgttag tttgccataa tgccttgata agagtgatca atgcttattt
     5701 tcatccgatg cctaatgagg ggttctttac attcatggta gataatacag aattggtttc
     5761 ttatgagtaa ttatgttttt gtgttattta aataaaatat ttcaaggcac tgcttttgct
     5821 tagcagtgcc ttttttaggt tatgacaatt cttttgataa tataaactca acctttagaa
     5881 atgaaattta attattttaa taaaa
//
LOCUS       DACDID010000142         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDID010000142 DACDID010000000
VERSION     DACDID010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.147"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IV236_002271"
     CDS             complement(<1..986)
                     /locus_tag="IV236_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP8772444.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IV236_002272"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IV236_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8772445.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="IV236_002273"
     CDS             1811..2965
                     /locus_tag="IV236_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8772446.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="IV236_002274"
     CDS             3180..4148
                     /locus_tag="IV236_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8772447.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="IV236_002275"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="IV236_002275"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8772448.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="IV236_002276"
     CDS             5178..5786
                     /locus_tag="IV236_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8772449.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DACDID010000143         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDID010000143 DACDID010000000
VERSION     DACDID010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.148"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..325)
                     /locus_tag="IV236_002277"
     CDS             complement(<1..325)
                     /locus_tag="IV236_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772450.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMR"
     gene            complement(425..934)
                     /locus_tag="IV236_002278"
     CDS             complement(425..934)
                     /locus_tag="IV236_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8772451.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1135..2004)
                     /locus_tag="IV236_002279"
     CDS             complement(1135..2004)
                     /locus_tag="IV236_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8772452.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2024..3145)
                     /locus_tag="IV236_002280"
     CDS             complement(2024..3145)
                     /locus_tag="IV236_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8772453.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3147..3542)
                     /locus_tag="IV236_002281"
     CDS             complement(3147..3542)
                     /locus_tag="IV236_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8772454.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3561..4358)
                     /locus_tag="IV236_002282"
     CDS             complement(3561..4358)
                     /locus_tag="IV236_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8772455.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4360..5148)
                     /locus_tag="IV236_002283"
     CDS             complement(4360..5148)
                     /locus_tag="IV236_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772456.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5162..5638)
                     /locus_tag="IV236_002284"
     CDS             complement(5162..5638)
                     /locus_tag="IV236_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8772457.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5710..>5903)
                     /locus_tag="IV236_002285"
     CDS             complement(5710..>5903)
                     /locus_tag="IV236_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772458.1"
                     /translation="SCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYY
                     SVDIPIHEVKYVYDYVKRA"
BASE COUNT         1811 a         1291 c          845 g         1956 t
ORIGIN      
        1 acctcatttc acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc
       61 tattacctat ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt
      121 ttatttttgt aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc
      181 ccagagtaaa ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc
      241 cccgaagctc aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt
      301 catcattctt atattcatca ttcataaaac caatccagct cctcgattaa gataatattt
      361 taatctcata caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat
      421 ttctttatac ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt
      481 tatctccgca aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc
      541 cttttgatgg atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag
      601 acaaggcgat ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt
      661 tttgcggcgt gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc
      721 catttaggaa tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg
      781 cattatcttt gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca
      841 gtggtacacc gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat
      901 aacgcaatcg caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt
      961 actttcttac gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc
     1021 acttatgata aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga
     1081 ccacattaag ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga
     1141 attgattcta acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa
     1201 cttaggtgtg acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc
     1261 taaaaaacgt ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa
     1321 aattataata tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc
     1381 cgcctcgatt tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg
     1441 catctcgata gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt
     1501 accagagaaa gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct
     1561 aaacccttca acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt
     1621 gtaataaaca acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc
     1681 agcattcaca tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac
     1741 aaaatcaaaa tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga
     1801 ttcacgatgg atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt
     1861 atagtgatta aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa
     1921 actcactctt tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc
     1981 acctgcttct attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt
     2041 cttccctgtc taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa
     2101 cagaagaaat atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga
     2161 aagtaacacg taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc
     2221 catgcatcgg taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag
     2281 cactaaaata atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg
     2341 tattaaaatt ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc
     2401 cgccaaaata atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta
     2461 tataaaactt aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt
     2521 aggttcctac ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt
     2581 ttgtcgtgat gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt
     2641 cagtcaatgc ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg
     2701 ttaaagtaat cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg
     2761 ttggattcac tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa
     2821 aaaaatctcc taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca
     2881 aaattaaatc acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa
     2941 aatgacgtaa aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta
     3001 tcggaacaga aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt
     3061 tccctttttc accattttta ttttctggtg aaatagttct actttcctct cccttgtgga
     3121 gccatatatt atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga
     3181 ttattcatat agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta
     3241 tcatttccta actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg
     3301 tgcactctgt cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca
     3361 gttaaaacaa ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca
     3421 tcaatttctt cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac
     3481 tctagtgtct ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc
     3541 ataattcaaa tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa
     3601 acaataacga gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat
     3661 gctgcagcag taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg
     3721 attttcatac taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa
     3781 gcaattaatg atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta
     3841 atcttttcac cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct
     3901 ctcattcgga aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga
     3961 ttattttcta aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc
     4021 aaaagagagt ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc
     4081 gcatcttttg cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg
     4141 gctgccagcc aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat
     4201 tcatcatcat ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca
     4261 ccttgctgtg tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc
     4321 ctaagatcct ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa
     4381 cccaacatca gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata
     4441 cacattaaat gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa
     4501 agcggctaag aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg
     4561 taacaacatc gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat
     4621 aaaccaatta ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc
     4681 aataaatact ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact
     4741 attcattttc ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc
     4801 atttaagatt ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat
     4861 tgctaatccg gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt
     4921 tccatagtcc ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac
     4981 tgtaccgcca actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc
     5041 ccaaaaaact gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat
     5101 aaatgctaat atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc
     5161 tttatcctaa ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa
     5221 tttctacacc agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg
     5281 ctctagaaga tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt
     5341 caattctaat accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa
     5401 atactttttc ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga
     5461 taattgatac agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt
     5521 tatttgcgac agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt
     5581 gcgtgacaat aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa
     5641 atacttcctc ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa
     5701 cttgataaat caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat
     5761 gtcgacacta taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt
     5821 ttgaaattcc tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac
     5881 taatcgttca attaagcaac taa
//
LOCUS       DACDID010000144         5884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDID010000144 DACDID010000000
VERSION     DACDID010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.149"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            515..2026
                     /gene="gadC"
                     /locus_tag="IV236_002286"
     CDS             515..2026
                     /gene="gadC"
                     /locus_tag="IV236_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8772459.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2039..3439
                     /locus_tag="IV236_002287"
     CDS             2039..3439
                     /locus_tag="IV236_002287"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8772460.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3604..5044)
                     /locus_tag="IV236_002288"
                     /pseudo
     CDS             complement(3604..5044)
                     /locus_tag="IV236_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5437..5884
                     /locus_tag="IV236_002289"
                     /pseudo
     CDS             5437..5884
                     /locus_tag="IV236_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1875 a          948 c         1112 g         1949 t
ORIGIN      
        1 cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa tcgttcatat
       61 ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt
      121 ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc
      181 tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt
      241 tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact
      301 aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa
      361 aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta
      421 gaaaagaggt agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg
      481 aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt
      541 cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc
      601 aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc
      661 agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt
      721 cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg
      781 gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta
      841 tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt
      901 cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc
      961 taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc
     1021 agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc
     1081 tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg
     1141 cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt
     1201 ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt
     1261 agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga
     1321 aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat
     1381 gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat
     1441 gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga
     1501 cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt
     1561 aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt
     1621 catttatctc gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa
     1681 aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg
     1741 tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc
     1801 aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac
     1861 tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga
     1921 ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg
     1981 agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat
     2041 gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc
     2101 aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat
     2161 tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc
     2221 tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga
     2281 aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt
     2341 aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac
     2401 gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa
     2461 acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc
     2521 tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt
     2581 cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga
     2641 gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat
     2701 caagggtctg aatgatttag ttgaagctca caataaacaa actgactata aagtatacat
     2761 tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg
     2821 ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt
     2881 atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt
     2941 atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag
     3001 tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg
     3061 tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac
     3121 tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa
     3181 agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg
     3241 atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt
     3301 agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca
     3361 agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac
     3421 gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa
     3481 aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata tgatattcat
     3541 ttgttacact ctcaatctta gctgttgttc tattcatcat tactcttatt gtaagtagtt
     3601 cagtcaaatt tgcttgttca attgatcgat aacagtatga attgtttgaa aagtaatcgg
     3661 atatataatt gttttctcac tacctaagcc ttcttggtat aaagaactat cgcaaagtat
     3721 aatccgctga gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt tacaaatgat
     3781 attactaata gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat agtataaata
     3841 cctaaagtct gacggagcta ctatgaacac ttcaattgga gatttccgtt tatgctcatt
     3901 taaaagactt aaactaatgg ttagtttacg aatagaattc tcattccaac gcagaaataa
     3961 attgttttgt tttcgccatt gttcaagaat ttcatgaact tctaagtaca aagcgcgttg
     4021 ctcttcattt aacaagtaag tcttatcagg ttgaaatatc tgaatatcag cccaaagtga
     4081 gcggataaac gggagaagtg ctttatcaaa aagtatctga ttttcttcag atatattcag
     4141 agataagata agtttttgac tgagctcaat caccaaaggg ttatctgcta catggttttg
     4201 ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat aatttctaga
     4261 gttgaataga aagtagatat aaaggcattc atcttcgtca tgacataaag agaaagaaag
     4321 ttgctctaat cctttctgta ttagctgaaa caagggagta tgtcgggcaa ctcttttttc
     4381 ttcctgacta aaatatattg ggttagtctt agatctctgc aaaccgatag cgatacctaa
     4441 taaaataaat tgtttttcaa ctgtagggta acttcttgaa aaaaaatgtt tttctacata
     4501 atcaatcaat tgttgaccgc tttgaataat tgatttattt agctggatca actctgtccg
     4561 atttgtctca tagattatcg ttagtaacaa acttcgataa tccttttcag gaatttcaaa
     4621 atggccatca gcatttaagt actgcatttg tttaaaaaaa ttaatcaact catttcgtaa
     4681 gttgtaaact ttgcttaacg aaatgaaggt agtatcagct atattactcc aattgtgttc
     4741 ccctgataaa taatgtccta gtacattgaa aaagtcagag cgctgataga tgagttgagt
     4801 gagtgccaac ctcgaaaacg aagagtcgaa tgcgactgta aagctacttc tagatttctc
     4861 cactgaaata atttgctgat ggagtatgtc ggtaatgcat tccaagtcgt tagtgacaga
     4921 cagtgagtgg gcaaacgttc aattgatctg ctatttcgcg aatctgaatg gtcccttttt
     4981 cgtgtaattg ctcacaaatg taaacttgac gaaaaatact tttctccaga taggtttcca
     5041 acatagtact cctcctagtc attttataca taactattat aactctacca atcggaatag
     5101 tcctgtaata tattgaatca aaaatataaa ttataaaata attagatggg gtatttggtt
     5161 taagtatccc ttatatagta ataaatttat aggtgtgatt ctcgaattaa tacccgaaaa
     5221 tgtgagagaa tttttatgga ttttttagga tacaaattaa ttatttttgc ttttatcctg
     5281 attaccagtc cagtactgtc ggtatcccat tttgcagcaa aatcatgttg ataaagtgac
     5341 aagaagactg atgagatata ttatccaaat tgagaagata attaatcgga ttcttgagta
     5401 taccctactt tttaagaaag tagggtatac aagcgtatga ataaagacat aactttccct
     5461 gtgatggacg ttcggcttgt tccaattacc aaaatacagg cgaacgatta taatcccaat
     5521 cgagtagcgt ctccggaaat gaacttattg gagctttcta tcattgaaga tggctttact
     5581 caaccattag tttgttatta caaggaaaaa gaagagaagt atatcttggt tgatgtgctt
     5641 cataggtatc gtgttgctaa ggaaagactc aacttaaaag ttgtaccggt aacagttatt
     5701 gataaaccca tataagaacg tatggcttca acaattcgac acaatcgtgc aagagggacc
     5761 catgaaataa gaggaatgga aattgtcatc caaagattag taaaaaatgg atggtcagac
     5821 aagagaatat ccaaagaatt aggcatggac attgaagaaa tttttcgctt taagcaagtg
     5881 tctg
//
LOCUS       DACDID010000145         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDID010000145 DACDID010000000
VERSION     DACDID010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.150"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IV236_002290"
     CDS             450..1604
                     /locus_tag="IV236_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8772461.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IV236_002291"
     CDS             1598..2020
                     /locus_tag="IV236_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772462.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IV236_002292"
     CDS             3143..4075
                     /locus_tag="IV236_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8772463.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IV236_002293"
     CDS             4092..4607
                     /locus_tag="IV236_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8772464.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IV236_002294"
     CDS             4903..5184
                     /locus_tag="IV236_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772465.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IV236_002295"
     CDS             5186..5602
                     /locus_tag="IV236_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772466.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2227 a          961 c          931 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtgtacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDID010000146         5658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDID010000146 DACDID010000000
VERSION     DACDID010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.151"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(187..864)
                     /gene="nth"
                     /locus_tag="IV236_002296"
     CDS             complement(187..864)
                     /gene="nth"
                     /locus_tag="IV236_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8772467.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(871..1569)
                     /locus_tag="IV236_002297"
     CDS             complement(871..1569)
                     /locus_tag="IV236_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8772468.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1837..3021
                     /locus_tag="IV236_002298"
     CDS             1837..3021
                     /locus_tag="IV236_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8772469.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3027..3470)
                     /locus_tag="IV236_002299"
     CDS             complement(3027..3470)
                     /locus_tag="IV236_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8772470.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3696..3983)
                     /locus_tag="IV236_002300"
     CDS             complement(3696..3983)
                     /locus_tag="IV236_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772471.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4154..5506)
                     /locus_tag="IV236_002301"
     CDS             complement(4154..5506)
                     /locus_tag="IV236_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8772472.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1493 a         1137 c          957 g         2071 t
ORIGIN      
        1 ctacatcggg tattttccca ataaaaataa caacttttat ttttatcgca ataaattgta
       61 ataagttacc atcctagcta ctgagtatgt acatttactt tttcatgcaa aaaagataaa
      121 cgaaacagct tttctccata ataacaagat aattcataca tttatttgca attaaagaaa
      181 ccacgtctac aggcgtggtt tcttttttag cgccttttct tttagacgca tccgattttt
      241 cccatcttga catattgata gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca
      301 atgatatcta ccaaaaaaaa tcagtgtatg atgtgtcttt acccataatt cttgtggtac
      361 tttccgcatc agtgtttctt ctacttccat gaccgtagca tctagtttgc agatcctcag
      421 gcgttttgag acacgttcca cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc
      481 ccccaatacg acatttgctg tctttctgcc tacaccagga agagacatca attcttctct
      541 tgacgttggc acttgcccat cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt
      601 ttttgcttta tttcgataca aaccaatcgt ttttatttta agaatgattt catcgattga
      661 agcttctgct aacgcatcgg gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt
      721 cactgataca tctgtcgcct gggcacttaa gatcactgca atcaataact caaatggatt
      781 attgtgcttg agttctccat gtgcttcggg aaacattcca tacatcgttt ctagagcttc
      841 cattgttttt tgtttgctta acatattccg ctactcctcc aaccaatttt tcagtgagac
      901 tttcggcaat tcttcttgtt gagctgactg ttctacttct ctttgaagaa gctgctgttt
      961 tcttcgtttt tggtcttctt ccacttgttg ttttgtacgt agattttttc tttcccacga
     1021 tagcaaaatg cggtcaacgt aattaaaact ataagcctga tttaatacgg cttcccgcag
     1081 tgctagcttc aaaatttccg gctgataatg gtcttcttct agccattgtc cgatccgctg
     1141 gaactcgatc gaagaaagcg ggcgtccaaa ttcttgttca aataattgat acactgactg
     1201 gatttctttt tcttgtgttt tttcttcgta ttttttttct tgtgttttta aatactcacc
     1261 taatagatca tagatggggt aaagattata tcgatctgct tttttaccgt tgacaatcgt
     1321 cgtttctatc ttgataaatc catttgttac gagacgatcc agtatttgaa aaatctgatc
     1381 ttgtttgatt cccatatcca tagcaatcat ctgcaaatca gggaaagtat ctccttcaag
     1441 ctgtgccata tgcaactgta gaacaaataa aaattcttca gattgtagac cgatcctatg
     1501 ataattttta agtaaaagat tagatatgac tgtttgtcca gcgcttaagt aattttctaa
     1561 attcaacatg atttctcctc cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa
     1621 gaacaggttt cttttctatt tacttcactg atcggtcaga tttacaatgt cgtctacttg
     1681 atttttgttt gttcatgttt ttgatgcgta tcttcacttt tcggaataaa taaattaagt
     1741 agatttcgct gttccttaga atgttgacaa ggaaacaaaa ttactataca ctatttttgt
     1801 ttccaagtta acattattgc ataaaggagt ttttacatgt acagtttcac aaataaacat
     1861 tctaaagttt ttggatacgt ctctgtctta ttcactggat taggatttac tatcgtcagt
     1921 cctgtacttc cgtttttaac gctgccttat tctcactcca tacatcagca agctttctat
     1981 atcactttgc tcatgtctgt ttatgctcta gctgccttct tatctgcccc cattctcggt
     2041 agcttaagtg atcattttgg gcgtcatccg atccttatta tcagtttatt aggatcaagt
     2101 atcggatatt tgctttttgg tttaggaaat tcgatatgga tgttgttcct tggtcgtatc
     2161 attgaaggat tgacggcagg agaaatctcc accttatatg cttactttgc cgacatcacg
     2221 gaacctaatg aaagaaccaa agtttttgga tggatgggtg ctttagtcgg tataggaaca
     2281 acgctgggtc ctattatagg gggtctatta gctgagttgg ggaattctgt tcctatattt
     2341 attggagcac tctttacttt cttaaatgcc gtttatggct acacatttat gctagaaagc
     2401 cttccaataa aaaaacgttc agtagattta agtttctccc atgtaagacc ttttcaccaa
     2461 ttacaacaat tattcaagat ttcatccgtt attcctttat tgacagcagg ttttgctgtt
     2521 tggttggcag ctggctctct tcaatctatc ttttctcaat tttcgattga cgcctttcag
     2581 tggaaggctg ggttggttgg tttaagcttc tctttgattg gaatcttgga tactgtctcg
     2641 caattgttca ttatgcctcg attgctcaaa aaattttcag aacagcagat cacaagaata
     2701 gggatgttca gtgaaatcct cgcctatcta ttcatcacat tatctggaat tcttctactg
     2761 ccgattttgt ttttatttgg gattatctgt tatggtttcg gtgattctat ttttactcct
     2821 gtattcaatg gccaactatc caattctgtc tcagataacc agcaaggtct tgtaatgggc
     2881 ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt
     2941 tatgctgcag caccatttct tccgacggta ttcggatttt tgcttatgat ttttgcctat
     3001 tatcactaca ataaaaaata gtagcttcat ttgctacttc gtataaattc tctgtatcga
     3061 ccgataaaac gaaatactgt gtcaaattct tcttttgaca tattttcaaa tacagtagca
     3121 tctcgctgta cccatttttg atggagttgc tcgtgtctat tccttatttt ttgtccgatt
     3181 ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc tttgataagt gtttatgatc
     3241 cctctagcta tcagtttttt tgttagctta ctgatggctc ctctagtcat gtaactagca
     3301 ttcgctaatt ttgtcacgtt aggatcaggg attttcccga tatgttcaat cacttctagt
     3361 tcatttagag taaatcctgc aaattctttt tccatttctg gtttattacg atgaataatc
     3421 tcattagcta aatccagata ttcttctatt agcagttgtc ttttatccat ttctacacct
     3481 tctttttctt tcattatatt gtttttttgt ttcctattca acaaagataa aaatattttg
     3541 taggaaaacc caatctgaaa ccaagaaaaa aaggggttga caaagcttgt cataacccct
     3601 ttttctgatt aaaagatagt aaagagacag tcatcggtaa ttgtgatgat tttttgtcat
     3661 cacttaagga tgagcggcct tttcaaaaac tgtctttaaa gatcatcatc agtttctagc
     3721 acatatggct gaccttcata tgtgtactct gtattcaatg tgccgtcatt ttcatctgag
     3781 tagatgcgtc tactgaaagg aaaacgatga gaaacattat ctttggcgtc atttgctggc
     3841 atcgtgtaat attcctcttg cgtttcattc aactgagaga gtacttgatc tgccaactca
     3901 tcaatattga tcgttaattt cttcgaagca cttttataat cttctaaaaa tgcatcttgt
     3961 tggacaaaat attctttatt catcttcttt ctcacctttc ttttcaaaat caaataaagc
     4021 tgcttgttct tttatgtaac agctttatct ttgctgctta ttgtgacaaa aaattcgaag
     4081 aaagacaact aattgtactt ttcacaaaaa aagacagagg gtcgtgacaa aattgtgtca
     4141 caaccctttt tctttattgt gcagtttcga tttcgcctag cgctgcttgt gcaagtaggt
     4201 tcaagtaatt ccatgggcgg tcaaaatgtg gttggaagaa gaaatctgaa agtgcgagat
     4261 cttctacagt cattttattt tgaacagcta aagacatggt atttgctgat tgtgtaatgt
     4321 cgtatttgga catgaactga gcacctacga tacgattcgt tcctttttca tagactagct
     4381 ccatcattac tttttcagta gttggcatga actctggacg ataattatct tcaaatacag
     4441 tcgcttggac ttctagatcg ttcaaagttg cgctttcttt ggtcacacct gtcgaaccga
     4501 tcgtccagcc aaataggtaa agtcctgaag ttccttgagt accacgatac gccattttat
     4561 gatctgtcaa gttatttccc acaagcaaac cttgacgaac ggcatttgtt gccaaaggaa
     4621 tatagttttg cgtgccactt ggattgtagt tgactactgc agagtctcca gctgcaaaaa
     4681 tgtctggatt acttgtctgc atgtattcat tcactttgat tgccccgttt ggcagtgtat
     4741 ctacttttcc ttcgatcagt tttgtatttg gacggaagcc cacacataaa atgaccatat
     4801 ctgtatcaaa tgtatctgtt ggtgtagcta cttgtttcac ttttccttct tcatctgtga
     4861 taaattcagt aacattttcg cctagcgcaa gagtaacacc gcggtctgtc aattcttttt
     4921 ctaagatatc tgtgaatggt ttatctagat atttattcaa gatgcgatct aatccatcaa
     4981 tcaatgtcac ttgttttcca gattcgacaa aagcttctac taattcaata ccgatatatc
     5041 caccaccaac aattactaca cgttttgctt ctttcgcttg tgcaataatt tcatttgctt
     5101 gattgtagtt tttgcatagg agtacatttt tcgcatcgat tccactaatc ggtgggataa
     5161 taggccatga accagttgtc atcactaatt tatcataaga aaccgtttct gtttctcctg
     5221 ttttcagatc ttttgccgtt actgttttat tttctgtatc gacgtctgtt acatcgtgtt
     5281 ccattttaac tgttgctcct aatgaagata attcttcagg atttgagtag aacaagcctg
     5341 ctgggtcctt tactactccg ccgacataaa gtgcgatacc gcaagataag aaagaaatat
     5401 tgtcatttct ttcatatact gtcacttctg cttctggatg attttttaaa atacttttta
     5461 cagctgcagt acctgcatga gtacagccaa ttactactac tttcatttat ggtccctccg
     5521 attaacattt tgtccttcga caaaactact ataacagaaa taaaacggat acaacagttt
     5581 aatgcttgtg aattcacaaa ggtttgtttg tagtttcatt ccttttcaag cataagaacc
     5641 caatatctac aagattct
//
LOCUS       DACDID010000147         5614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDID010000147 DACDID010000000
VERSION     DACDID010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.152"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IV236_002302"
     CDS             complement(59..2008)
                     /locus_tag="IV236_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8772473.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IV236_002303"
     CDS             complement(2050..3108)
                     /locus_tag="IV236_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8772474.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IV236_002304"
     CDS             complement(3227..3682)
                     /locus_tag="IV236_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772475.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IV236_002305"
     CDS             complement(4266..5069)
                     /locus_tag="IV236_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8772476.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DACDID010000148         5531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDID010000148 DACDID010000000
VERSION     DACDID010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.153"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..1036
                     /locus_tag="IV236_002306"
     CDS             44..1036
                     /locus_tag="IV236_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772477.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1096..2517
                     /locus_tag="IV236_002307"
     CDS             1096..2517
                     /locus_tag="IV236_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8772478.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2510..4009
                     /locus_tag="IV236_002308"
     CDS             2510..4009
                     /locus_tag="IV236_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8772479.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4048..5442
                     /gene="uxaC"
                     /locus_tag="IV236_002309"
     CDS             4048..5442
                     /gene="uxaC"
                     /locus_tag="IV236_002309"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8772480.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1809 a         1029 c         1148 g         1545 t
ORIGIN      
        1 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
       61 tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
      121 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
      181 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
      241 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
      301 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
      361 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
      421 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
      481 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
      541 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
      601 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
      661 tgtcaatcta gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
      721 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
      781 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
      841 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
      901 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
      961 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
     1021 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
     1081 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
     1141 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
     1201 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
     1261 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
     1321 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
     1381 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
     1441 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
     1501 actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg tttcaaatta
     1561 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
     1621 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
     1681 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
     1741 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
     1801 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
     1861 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
     1921 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
     1981 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
     2041 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
     2101 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
     2161 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
     2221 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
     2281 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
     2341 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
     2401 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
     2461 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
     2521 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
     2581 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
     2641 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
     2701 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
     2761 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
     2821 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
     2881 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
     2941 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
     3001 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
     3061 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
     3121 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
     3181 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
     3241 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
     3301 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
     3361 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
     3421 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
     3481 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
     3541 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
     3601 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
     3661 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
     3721 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
     3781 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
     3841 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
     3901 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
     3961 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
     4021 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
     4081 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
     4141 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
     4201 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
     4261 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
     4321 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
     4381 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
     4441 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
     4501 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
     4561 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
     4621 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
     4681 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
     4741 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
     4801 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
     4861 cttacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
     4921 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
     4981 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
     5041 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
     5101 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
     5161 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
     5221 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
     5281 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
     5341 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
     5401 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
     5461 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
     5521 agacggacca a
//
LOCUS       DACDID010000149         5438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDID010000149 DACDID010000000
VERSION     DACDID010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.154"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV236_002310"
     CDS             131..505
                     /locus_tag="IV236_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8772481.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV236_002311"
     CDS             complement(619..1248)
                     /locus_tag="IV236_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8772482.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV236_002312"
     tRNA            complement(1382..1454)
                     /locus_tag="IV236_002312"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IV236_002313"
     tRNA            1668..1741
                     /locus_tag="IV236_002313"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IV236_002314"
     CDS             1696..1845
                     /locus_tag="IV236_002314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772483.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IV236_002315"
     CDS             2338..3666
                     /locus_tag="IV236_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8772484.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IV236_002316"
     CDS             3868..4701
                     /locus_tag="IV236_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8772485.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..>5438
                     /locus_tag="IV236_002317"
     CDS             4717..>5438
                     /locus_tag="IV236_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8772486.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANI"
BASE COUNT         1831 a          839 c         1073 g         1695 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattat
//
LOCUS       DACDID010000150         5296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDID010000150 DACDID010000000
VERSION     DACDID010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.155"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..1332
                     /locus_tag="IV236_002318"
                     /pseudo
     CDS             44..1332
                     /locus_tag="IV236_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1458..2537)
                     /locus_tag="IV236_002319"
     CDS             complement(1458..2537)
                     /locus_tag="IV236_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8772487.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2640..3080)
                     /locus_tag="IV236_002320"
     CDS             complement(2640..3080)
                     /locus_tag="IV236_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8772488.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3137..3547)
                     /locus_tag="IV236_002321"
     CDS             complement(3137..3547)
                     /locus_tag="IV236_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8772489.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3727..4161)
                     /locus_tag="IV236_002322"
     CDS             complement(3727..4161)
                     /locus_tag="IV236_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8772490.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4261..5109)
                     /locus_tag="IV236_002323"
     CDS             complement(4261..5109)
                     /locus_tag="IV236_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8772491.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1944 a         1016 c          884 g         1452 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 agacccagaa gaaaaaccag atgtgactcc agaaccagat acggattctg gaaaccaaac
      781 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
      841 agccccagaa gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac
      901 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
      961 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
     1021 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
     1081 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
     1141 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct
     1201 gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg
     1261 agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac
     1321 agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc
     1381 cagaaaatga ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat
     1441 ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt
     1501 ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa
     1561 acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct
     1621 gttttgctgg acatcaactt cggattcagc acataagact caattagatg gacaatcaaa
     1681 atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat
     1741 agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg
     1801 atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac
     1861 gtatttaaaa gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac
     1921 ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat
     1981 cttgagaata caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac
     2041 atagcaaacg ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg
     2101 ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa
     2161 acaggatctg catcttttgg ctgatgctcg taaaaattaa taacggactg gatcatgtca
     2221 aatgtttgat gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg
     2281 atcagtgtat aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg
     2341 atttgaacaa gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc
     2401 atacttctaa ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt
     2461 ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa
     2521 ataaacacgt tattcattat atgatggaat atgaaaataa gaaagagcaa aaagaaaaac
     2581 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
     2641 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
     2701 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
     2761 gcttgttttt gcaacgcagc accttgagca gtcagcgtaa catttacgat tctttcatca
     2821 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
     2881 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
     2941 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
     3001 ggagtgtagt gtctaatgat ttctttagag gcggcatata aaggaaaaca tacttgatta
     3061 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
     3121 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
     3181 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
     3241 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
     3301 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
     3361 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
     3421 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
     3481 cgggattatg cacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
     3541 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
     3601 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
     3661 gcgctttctc gaatagcaaa catatacaaa aaatatcaga gacaaaaaat aatctcatca
     3721 taaacattac atatatttct caaaaagttg ttttctttct tcaaaaatta cttcacgatg
     3781 gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc
     3841 aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt
     3901 atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc
     3961 agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtaccata
     4021 caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat
     4081 ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc
     4141 gatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat
     4201 tcttttatta taccagctaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac
     4261 tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat
     4321 gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc
     4381 agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag
     4441 cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg
     4501 ttcactcatg accataacag atttctttcg attgatacct gtttcgatat aagtcatgac
     4561 aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaataaa
     4621 cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg
     4681 gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt
     4741 atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg
     4801 atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc
     4861 gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac
     4921 gatattcaac actaaaataa ctatagctcg gtcaaagcct aaagcagctt ccagtaatat
     4981 tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc
     5041 gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt
     5101 ttctttcatt tattcacttc cttaattcat taataatact cttatcttat tataacttac
     5161 ttttcacctt aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt
     5221 tcattcttct agaatatact tctacgccaa aaactgttaa aaaactttta ctttagaaaa
     5281 ttggagaaat tgatcg
//
LOCUS       DACDID010000151         5176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDID010000151 DACDID010000000
VERSION     DACDID010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.156"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..673
                     /locus_tag="IV236_002324"
                     /pseudo
     CDS             1..673
                     /locus_tag="IV236_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            732..1172
                     /locus_tag="IV236_002325"
     CDS             732..1172
                     /locus_tag="IV236_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772492.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1184..1852
                     /locus_tag="IV236_002326"
     CDS             1184..1852
                     /locus_tag="IV236_002326"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8772493.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1916..2539
                     /locus_tag="IV236_002327"
     CDS             1916..2539
                     /locus_tag="IV236_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8772494.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2595..2981
                     /locus_tag="IV236_002328"
     CDS             2595..2981
                     /locus_tag="IV236_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8772495.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3194..4585
                     /locus_tag="IV236_002329"
     CDS             3194..4585
                     /locus_tag="IV236_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8772496.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1714 a          860 c         1087 g         1515 t
ORIGIN      
        1 atgaattttt tagttgtttt aaaagatact ctgattgagc gatctacatt tctatatgtt
       61 caattgcagg aaaataaaac attgctgcat ttttcaccag agttccatct agaggggcta
      121 acattaggag aaatctatca agcagaaggg ctgtcggctg ttttgcactt ttttaaggta
      181 gaattggaat taccagtaaa agattatatc gagctttctc ttgaatcttg ggatcgagca
      241 gtggttgaac tgtttccaga aggtttaatg atcgatacaa acgatgggaa gatccatctg
      301 actgcagcag aactgcaaaa acaaatgcgt tatcaaatag atgacgaaaa ttctttttct
      361 atttttagac gtcagcaaaa gattctaaaa agctttttaa aagtgatcag taaaaaaacg
      421 gaatctgatc aaaacgacac agctgttgaa aaaatatcca ggtctgcttc acacgtctat
      481 tcaatttcct caattgatta cgatgatcca tcgttttata cgtatgcaac agctaccatc
      541 taagaagctg tttcttccat tgacggacac atttcgagta gtcaacagag aagataaaaa
      601 gaaagtgatt gtgattgact ttaccagaaa tcggaatgtc cttcaccaag ttgcaatgga
      661 aaaggccaat taaggctttt tctttttgga tttttttgtc ataataaata ttagatgatg
      721 gaggagataa tatgatgcaa acatggttag aaaggctaat acgatttcag cagaactatc
      781 acaaacttat gcagggaaga tatgcgaaat ttgatcagtt gaatcgcggt ctattgatcg
      841 ctgcgcttgt ctttagtttg ctaagtcgtt ggttgcccta tagaatcgga caattgttgt
      901 tcatcgtctt tttcggatgg atgatatttc gtttcctatc aaaaaaaatc taccccagac
      961 taaatgagaa tcaacgttat ttgaaatatc ttgatagagt caaagaaaaa tatcatcaga
     1021 taaaaaacaa aaagcaagtc aaaatggatc gaaaaagtta tacttttttt gagtgcccga
     1081 attgtcatca aaagcagcgt gcacctaaag gaaaaggacg aatacgtgtg acatgcaaaa
     1141 catgtggtat aaaattcgaa acgaatgttt aagggagaaa agaatgaaat tagtattatt
     1201 acgtcatggc gaaagcgaag caaattttga aaattactgg acaggatggc tagatgtcgc
     1261 cttaacagaa aaaggaaaag aacaagcagc agttgccggt caaaaaatgc ttgaagccgg
     1321 acttcaattc gatgccgttt atacctctgt tttgaaacgt gcgattctta ccggacaagt
     1381 cgcactagaa gaaatgggtc agttatggct tcctataata aaaacatggc gtctaaatga
     1441 acggcattat ggggatcttg tgggtaaaaa caaagataag atgaaagaaa tatacggaaa
     1501 agatcaagta aaaacatggc gcagaggata tacggaagtt ccgccgttaa ccgcagaaaa
     1561 tagatttgac cgcagatatg atcacttgga tccgcatttg atcccatacg gagaaagtct
     1621 ggaaatgaca gtgaatcgga tcattccctt atggcaagat catctagcac caaaactaaa
     1681 agatcggcaa gatttactag ttgtgggtca tggtaatagc atccgagcac tgacaaaata
     1741 tttagaagat gttcctgaag atcaaatgga tacgatcgat atcccaaatg cacaaccaat
     1801 ccagtataca ttagcagatg atttgacaat tctggacaaa aaaatacttt aaagaacacc
     1861 tttatctaaa agtgagagat agaaaaaaga agcaaaggaa tggaacaaaa ggaacatgaa
     1921 aaattatata tgggactttg atggtacatt gtttgatacg tatcccgcaa tggtagatgg
     1981 tgcatggcaa gcactaaaag attttggtat cagtatggac aaaaaagaaa tctacttcaa
     2041 aatgaaaaag tattctacta gttatttgat caatgaatca aacttgaatg ccagagagtt
     2101 taatgaatta tttcaccgat atgaaaaaga aagtacggaa gtttcccgtc catttccaga
     2161 aacgaaacaa gtactggaaa tgctaaaaga caacggcggt cgtcatttca tcctgaccca
     2221 ccgtttgaca gaatcaacat ggggcctgtt aaaagaacat cgattggcac atctgattga
     2281 ggaagtagta gggatcgatc aggactttcc tcgtaaacct gatccagcat ctttaaatta
     2341 tttgatcgat acttttcatt tagaaagaac agacacgatg atgatcggcg atcgtcgttt
     2401 agatatcgaa gcagggaaaa atgcaggagt cgctacttgt ctttacgaca tcgatcattt
     2461 tctgggtgag atcccggcag attatgtggt aggaaatctg aatgaaatcc ttaccttgca
     2521 gagcatcatt aaaaagtagt atcccctttc aaaggagata tgttataatt ttcataaagg
     2581 agtgataaaa ttatatggaa atcagaagac aaaaagaagt tgtagaagca tttcattatg
     2641 atatgagatt gccgaaccaa gaagtagaaa cagatttgaa agtaggcttc tcgcctttaa
     2701 aagaaacgat cgaaaactat ccgaaagaac atagcatcgt aggagcacgc ttagagttcc
     2761 gtttagtgtt tgatacatat gttttatctg gaagtgtgag tcagatcaat catatcctca
     2821 atcggaaaat tgaaggtcaa aatgatgtca cacaagaaga agtggacgag ctagtagcac
     2881 cgttattcga tatcgtacgc cgaatgtctt atgaggtgac agagatcgcc ttagatcagc
     2941 ctggggtaaa attgaatttt caatcagaaa aacaagaata gacagtaaaa acatacctgc
     3001 gatagattaa agctatcaca ggtatgtttt tttattacta attgtaagca aaaagaatgt
     3061 catttcgata aaaatcgtct aaaatataaa agtcactgaa aaaatttttt tcaggaggat
     3121 ggttgaagaa tcattctcgg gaacctttac ttccttcgat ggacttgtca ttaataatat
     3181 gaggaggaga aaaatgacaa cactcatcac agttttagtg gttctagttt ttgcggcatt
     3241 attatatgtg ttctatcgta tgcaaaagcg ccatacaaaa ttttcaacac gcgtctttac
     3301 agcattagga gttggtatcg tctttggcgg tctgcttcaa ttgatctttg gtgtagacga
     3361 caaagtcgtt actcaatcaa tggaatggat cgggattgta ggtcaaggat atgtatcatt
     3421 cttgcaaatg ctagttatgc ctttagtgtt tatttcgatc attggtgcat tcacaaaaat
     3481 gaaagtttct gaaaagctag ggaaaattag tgcgactgta ttgacttctt tacttggaac
     3541 aacagctatt gcagcattga tcggaattgg ttcaacaatg ctgttcggac tccaaggagc
     3601 taaattcaca caaggagctg cagaaacagc acgaatctct gaactggcaa cacgtcagga
     3661 aacagttgaa aacttatcga ttccacagca gattttatcc tttatcccta aaaacgtttt
     3721 tgctgattta gcaggtagtc gtgcaacaag tacgattgga gtcgttattt tcgcggcatt
     3781 tgtcggaatc gcttatctag gcgtgaaaag aaaagcgcca aaagaagcag cattcttttc
     3841 agatttagtc gacagtctgt ataaaatcat tatgcggatc gtgacgcttg ttttgcgttt
     3901 gacaccttat ggagtatttg ctttgatgac aaacgttttg gcaacaagtg attttgcagc
     3961 tatcgtcaat ttaggtaaat ttgtcgttgc ttcttacgta gcattgcttg ctgttttgat
     4021 tgtccactca ctgattttga ttggtgcaaa agtcaatcca atcaactatt tcaaaaaagc
     4081 ctttccggta ttgagctttg cgtttacttc acgttcaagt gctggagctt tgccattgaa
     4141 tatcgagact cagacaaaag ctttaggagt agatgatgca acagctaatt tctcagcaag
     4201 ttttggtttg tctattggac aaaacggctg tgctggcgtt tatccagcta tgttggcaac
     4261 gatcgtcgca ccagcagctg gagtgaatgt ctttagtttg gaatttattt tgatgcttgt
     4321 ggcaattgta acaatcagtt cttttggtgt agctggagtc ggtgggggcg caacatttgc
     4381 ttcactgatc gttttagggg caatgaactt gccagtagca atcgttgggt tagttatctc
     4441 tgttgaacca ttaatcgata tggcacgtac agcagtaaac gtcagcggta gcatggtagc
     4501 tggtatcgtg acaagtgcac gcatcaaaga tttagatcgc gatgtcatca atgatgattc
     4561 taaagtgatc gatgtgaatg catagtcaaa aagaggttgt gatagaagtg gtcagcttta
     4621 agaaataaga agaagtttcc taaaaatagt ttctcaaatt tttgtgaaat ttcggcttat
     4681 ttccgagaag ctacttctga aacaccgtga ataagaggtt gtgaaaaagc tgccctgcat
     4741 caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg gttgagctta
     4801 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgtttc
     4861 actctgagtc acaaagagca atttcaaaaa atagcttctc atgtttgttg aatattgtgt
     4921 cttgtgaccg aggagttgtc aggagaacac cgtttaccag aggttgtgac ttgtttttag
     4981 tcacgacctc tttttttatt tattccatat aaatacacat tatttattcc gaaaagtgaa
     5041 agcgtaaata acaaaaacaa ccaacttatt tattattaaa gaaaagcgta tttaagtagg
     5101 acgattctgt tgagagcttt cgtagatttt cccattttcc tatggtaaac tgacaaagtt
     5161 gagaatgtta gaacag
//
LOCUS       DACDID010000152         5135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDID010000152 DACDID010000000
VERSION     DACDID010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.157"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..171)
                     /locus_tag="IV236_002330"
     CDS             complement(<1..171)
                     /locus_tag="IV236_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772497.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQG"
     gene            complement(173..1612)
                     /locus_tag="IV236_002331"
     CDS             complement(173..1612)
                     /locus_tag="IV236_002331"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8772498.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1797..3743
                     /locus_tag="IV236_002332"
     CDS             1797..3743
                     /locus_tag="IV236_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8772499.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4007..4879
                     /locus_tag="IV236_002333"
     CDS             4007..4879
                     /locus_tag="IV236_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8772500.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1616 a          903 c          924 g         1692 t
ORIGIN      
        1 tccttgaagt ccacgtgcta aattatttgg tttataacca agagttttca tagcttcatg
       61 aactttttgt tttgtttttt ctgataaata tcccttatta ttaatgacgc gtgaaacggt
      121 tgttgggcta actccagcta attcagcgac atcagttaat tttgctacca ttttaatatt
      181 ttaattcaaa gtattttcca gtaggttcgc ctgatttgat ttgaatacca ttttggtgat
      241 tttcagggaa tacacgacta gtaaacactt tttctccttt attaataaaa atctcaatga
      301 ttgagttatc tacaaaaatg ttagcagttg tttctttagt atcaattgag cattcacgaa
      361 tcgtaccaaa gtcagtggca tattgaatgc ctgctttgct gcgatctaaa ataatttttc
      421 cttctttggt atctacaatc aagctaagtc catttccttt actatcagag aaaagaagta
      481 actccgattt ctgatttgga ggaaaagtta gttcaagctc ataagtatta tttgtttctg
      541 cttttgctgt aaattgttca gaactagcac gtaatgatgt aatagcttct actggatatt
      601 ggtaaagttt accatctttt attgaaagtt cttttacaag actcattgct ccctgataat
      661 catacttatc agttggataa tcgatatcag gtagtccaat ccaactgaca atcagagcac
      721 gaccatcagg agtattgaat ccttgagtcg catacgcttc aaatccataa tcaagatttt
      781 gaatttctga agcgcccaca agttttactt tttctatgtc aagcgattga cagatcttaa
      841 aagtattcgg atagatattc ccataattta gctcagattt atccaaccct tgaggacaat
      901 aaatgagaac aggttttttg tctacaaata ctagatttgg acactcaatc atgtattctg
      961 aaccagtgcc gccaaaatca aggttgccaa cttcctccca tgtttccacg ttattgtcat
     1021 ccgctttgta aagtttgata aagcctgatt tttctaaatt ttgtgctcca acaatcgcat
     1081 acaattgtcc ttcataatta aacatttgtg gatcacgaaa atgatcagtt acatcagcag
     1141 gtttatgaat aagaacattc tcacctttcg taatgttgta gtctttatcc atccatgctc
     1201 cgatttgaag tgtatcacgt tcccaatttt catttcgaac gtttcctgta tagagtaaga
     1261 atagattccc ctctatttca tatgcagaac cggaataagc gccgtggcta tctcgttttg
     1321 tgtctggaag aagttctgct cctgttacat gaaaatgaac taaatcatct gattcattgt
     1381 gtacccattc tttcaaccca tgagcagctc caaatggcca attttgataa aaaagcgtat
     1441 attttccgtt aaaaaaagaa aaaccattag gatcgtttaa caatcctgtt ttaggttcga
     1501 tatgatagct agcacgccaa ggtgatcgtt ttacattatc cttaatttta tccatctcat
     1561 ctttagtcca atcagaataa gcacgatatc gtatttctcg aggtaagttc atattttact
     1621 ccttcaattt atctttttac aactagtatg gtaaacgttt tccgttgaaa aatcaatcat
     1681 ttcatttaaa aaaataaact atatgacaaa cgtttgacat atttttaaga tatgctaaaa
     1741 taaatttgca tttcagtgaa ccgttttcac gtaaaaataa aaaaggagtg attcgaatga
     1801 attacagtaa agtagcttcc gacgtaatac aagcggtcgg aaaagataac cttattgcag
     1861 ctgcacactg cgctacacgt cttcgtctcg tactaaaaga tgacacgaaa gtgaatcaaa
     1921 aagctttaga cgaaaatcca gatgtgaaaa gcacatttaa aattaatgga caatatcaag
     1981 tgattatcgg tgcaggtgat gttaatttcg tttatgacga acttatcaaa aaaactggac
     2041 tttcggaaat ttcaacagat gatttaaaac aaattgctag taaagataaa aaattcaacc
     2101 ccattatggc attaattaaa ttattatcag atatttttgt tccaattata cctgcccttg
     2161 ttgcaggtgg tttattaatg gcattacaaa actttttaac agctgctgga ctttttggac
     2221 caaaatcaat tgaagaaatg tatcctgcga ttgaaggtat ttcctcaatg atccaactga
     2281 tgtccgctgc accattcatg ttcttaccaa ttttagtagg tatttcagct gctaaacgtt
     2341 ttggagcaaa ccaatttctt ggtgctgcaa ttggtatgat catgacaaca ccagatcttg
     2401 gtggctcaac tgaatattgg aatatttttg gttatcatgt ttcacaaaca aactatgctt
     2461 atcaagttat tccagtttta gcagctgttt ggatattatc tatattagaa aaattctttc
     2521 ataaaaaatt gccatcatct attgacttta cattcacgcc attattatca gtaatgatta
     2581 ctggattttt gactttcaca gttattggcc ctgtaatgtt attgttgtct aatggaatta
     2641 cagacattat tgtttggcta tataacacta ctggatttat tggaatgggt atttttggag
     2701 gaacatattc acttatagtc atgactggtt tacatcaatc atttccagct attgaaactc
     2761 aattactttc agcgtggaca aatggtatcg gacatggaga ctttatcttt gtagttgcat
     2821 ctatggcaaa cgttgctcaa ggtgctgcaa catttgccat ttggttctta actaaaaatt
     2881 ctaagacaaa aagtcttgct tcctctgcgg gtgtctcagc acttcttgga attacagagc
     2941 ctgccttgtt cggagttaac ttaaaatacc gctttccatt cttttgtgct ttaattggtt
     3001 caggaattgc tacagcagta gcaggattac ttaaagtagt tgctgtttca cttggttcag
     3061 caggtttctt gggatttctt tcaattaatg caacatctat tccattttac gtattgtgtg
     3121 aattgattag ctttgtagtt gcttttgcaa ttacttactt ctacggtaaa acaaaagctg
     3181 ctggtatctt taaagcagaa gaaattgctg aacaaacacc agttaattct tcaaaagtag
     3241 caacaacaga tccaatatct actgatgttg aatcaacaca aacaaaagtt tcatcagaaa
     3301 caattgttag tcccatttct ggtaaagtga ttagtctttc ttcagtaaat gatccagtct
     3361 tttcatcaga agctatggga aaagggattg ccattaaacc aaatggaaac acggtccatt
     3421 ctccagtaga tggtactatt caactcacgt ttgaaactgg acatgcttat ggtattaaat
     3481 caaataatgg tgctgaagtt ctaattcata ttggtattga tactgtatca atgggaggaa
     3541 aaggatttac acaaaaagtt actactaatc aaaaagttag aaaaggcgat attcttggag
     3601 catttgaccg ttcaatgatt gagaaagaag gtcttgatga tacaacaatg attattgtga
     3661 ccaatacttc ggattattca gaagtaaata tagtagctga aaatacagtc actgagggta
     3721 ctgatttact tgaaatcaaa taaatatatt tccctgagta aagatggaat tttctttcca
     3781 aaatcaaata atatagatta atcaaatatt tgagaataac attttattta atcttttcat
     3841 atatcaattg tatgaaaaat tattagaatt ttaaaaaatg ataaaaattc aaaaagaaaa
     3901 aatagtagtt atttacttgt catgttacta aataaaaaaa taatttaaat atgatatata
     3961 gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc tatacggtgc
     4021 aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa
     4081 ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag tcattgactt
     4141 cttcaaacaa taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga
     4201 tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat tggcatggca
     4261 aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt cttggacaac
     4321 agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag gattgagcag
     4381 tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag aagggcgctt
     4441 tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc atccaaaaga
     4501 tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg ccgctggacc
     4561 cgccatcgaa aaacgcttag ggaaaaaagg acaagaggtt gcagaagatg atccttattg
     4621 ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga tgttttctcc
     4681 agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca
     4741 cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat
     4801 cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat tggctcgaga
     4861 agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag
     4921 atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt ttttgtttta
     4981 cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg aaatttcgat
     5041 agccgtaggc cacttgtatt gtgcaatagt tttttacacg ccgtttgtat aattaagcga
     5101 gacaaataaa aaagccattt atgttacact caaat
//
LOCUS       DACDID010000153         5100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDID010000153 DACDID010000000
VERSION     DACDID010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.158"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..148
                     /locus_tag="IV236_002334"
     CDS             <1..148
                     /locus_tag="IV236_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772501.1"
                     /translation="FAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYM
                     KKTL"
     gene            complement(226..759)
                     /locus_tag="IV236_002335"
     CDS             complement(226..759)
                     /locus_tag="IV236_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8772502.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(853..2031)
                     /gene="mutY"
                     /locus_tag="IV236_002336"
     CDS             complement(853..2031)
                     /gene="mutY"
                     /locus_tag="IV236_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8772503.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2024..2821)
                     /gene="recX"
                     /locus_tag="IV236_002337"
     CDS             complement(2024..2821)
                     /gene="recX"
                     /locus_tag="IV236_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8772504.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2928..4309
                     /gene="rlmD"
                     /locus_tag="IV236_002338"
                     /pseudo
     CDS             2928..4309
                     /gene="rlmD"
                     /locus_tag="IV236_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            complement(4483..5061)
                     /locus_tag="IV236_002339"
     CDS             complement(4483..5061)
                     /locus_tag="IV236_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8772505.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1463 a         1049 c          918 g         1670 t
ORIGIN      
        1 ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca cagctgaaac
       61 tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg ctggcatgcc
      121 tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa
      181 gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact tacgcgacaa
      241 ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc catctccatt
      301 attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg
      361 gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc gtttctcgcc
      421 gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct catcgtccag
      481 caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga tcattgctat
      541 tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg
      601 gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag agtgttcgct
      661 tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact
      721 ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc cttaagcagc
      781 attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta tatcaataaa
      841 aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact gatctaccag
      901 tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca accacttcga
      961 agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag
     1021 aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc gtgtctgcca
     1081 aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta ctaggtcatc
     1141 aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat actcctcttg
     1201 tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc tatctctttc
     1261 ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat aaacgtcttt
     1321 tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt tgttagctaa
     1381 acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac agatagctga
     1441 acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa tgattttccg
     1501 catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt caatgcaaaa
     1561 caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc
     1621 gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc taatttcttc
     1681 aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag cagcttggat
     1741 gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct tttcttctgg
     1801 agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa aataatcgat
     1861 cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac gataaggatc
     1921 acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa tgaactgatc
     1981 ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca ctttcctctt
     2041 ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga tataagcttt
     2101 gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc catagtttat
     2161 ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc ggaagatcag
     2221 ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt tgttggattt
     2281 ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt tcggctgtct
     2341 gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt tcaatgattt
     2401 caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct ttgtcactta
     2461 agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca aggtccaatt
     2521 cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt tcttttaagt
     2581 atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt gctaggtgaa
     2641 aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg tctaattccc
     2701 gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa gcttctcctt
     2761 cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta acggttaaca
     2821 taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc gtaatcaggt
     2881 attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa
     2941 ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata aatggtgaag
     3001 gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca aaagaagaag
     3061 tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta ataaaagtca
     3121 aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa tgcggtggct
     3181 gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat ctgcttttgc
     3241 aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg ccgacgatcg
     3301 ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga aaaaataagc
     3361 agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta gtccctataa
     3421 cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt gttcaactat
     3481 tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc ttccgcacaa
     3541 tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt actcgcagta
     3601 aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg
     3661 tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg gatgaaacga
     3721 tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact ttcgatcttt
     3781 ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat ggcgaaggaa
     3841 tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc ggagttggga
     3901 cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat accgttcctc
     3961 aagcaatcgc tgatgcaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     4021 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     4081 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     4141 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     4201 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     4261 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     4321 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     4381 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     4441 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
     4501 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
     4561 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
     4621 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
     4681 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
     4741 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
     4801 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
     4861 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
     4921 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
     4981 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
     5041 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
//
LOCUS       DACDID010000154         5060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDID010000154 DACDID010000000
VERSION     DACDID010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.159"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(103..324)
                     /locus_tag="IV236_002340"
     CDS             complement(103..324)
                     /locus_tag="IV236_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772506.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(321..611)
                     /locus_tag="IV236_002341"
     CDS             complement(321..611)
                     /locus_tag="IV236_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772507.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(608..1792)
                     /locus_tag="IV236_002342"
     CDS             complement(608..1792)
                     /locus_tag="IV236_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8772508.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1974..3377)
                     /locus_tag="IV236_002343"
     CDS             complement(1974..3377)
                     /locus_tag="IV236_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8772509.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3399..4172)
                     /locus_tag="IV236_002344"
     CDS             complement(3399..4172)
                     /locus_tag="IV236_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772510.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4182..4559)
                     /locus_tag="IV236_002345"
     CDS             complement(4182..4559)
                     /locus_tag="IV236_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772511.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4580..4894)
                     /locus_tag="IV236_002346"
     CDS             complement(4580..4894)
                     /locus_tag="IV236_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8772512.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1473 a         1012 c          880 g         1695 t
ORIGIN      
        1 atgttttaat taaaagagat ttaaattgat catttgtaaa catattcata aatcctttca
       61 taacaaaata tatacttcct attttatcat gaaaaatatc tgttaagcac caatgattcg
      121 gttaaataag tttaataaca catctttcac gccagctgca ttgaacacaa gtccgacggc
      181 gattagagca ataatcaaga agccaatgag ttttgaaaat tctcgtttaa aacctaaaaa
      241 cagtccaatg acaacaatcg ctaacaatac caatgactgt gcattatcca agaaccagtt
      301 gtacaaattc tgcccaaaat tcatgtgatt gtcctccttt ccttaagata tttttctcga
      361 taatagatat agctttttac acgccttaat aattgaaaag gggttaaatg ctctccatag
      421 acggttactc gattggtata attattataa aatctcacct tgtaaaaatc ataggtatag
      481 atggtatcac cagcttgccg ttttccctca ataatgagag acgtatattt ccctgtattt
      541 tgtatatacg cttgaaactc aatgccacac tgaaacccta acttctttaa ttcttttgtt
      601 aaaaggctca tgtaatcaaa tcctccatgg ctagatgttg ttgttcaatt aatttctcat
      661 gtttttctgt cagtttggct tcctgaatca tggtggagat aatggttgta ccgttgagaa
      721 catctaaaat gcttgcgatt tttaatgtag gggcgacttg atgatgtaac cagttcaatg
      781 ttcgttcaaa agaataaggc tctggctgtg tggttaggcg taatgctcca cgatttttac
      841 cgataaacca ttcccaacgt tcattagttt tccaatcact ccgacgttta gtgtcgtctt
      901 tatctgcaaa gcggatatag cgattgataa tttcaaaggc tgttttttcc gcctgttgat
      961 gtgccaataa atctttcatg gcttgggtgg ctcggtcatt ttttaaccga atttcaaacc
     1021 gattctttac ttctgcgtct tctaagggaa catcattttt cacgtattgc tcataatctt
     1081 tctcatatat acagaaatag acttcacttt ttaatgagcc aatataaagt gtgtttccca
     1141 tgtatggttt ttcatcacgg tgaaccaatt caccactgcg ataattttta aagcttcgaa
     1201 agacggaaat gcattcttcc tgtttacatt tctttgccaa atctggaata ttcaaaatcc
     1261 ctgttttatc gttgatagct aaatccaagc gtttgaaaat acctttatgt tccatgcaat
     1321 cttgaaagaa atcataccag ctccgatgtt gagccaatag aaagttttca aattgccgac
     1381 agccttttcc tttcagttct aataaaacac ctttacttac atcatgagag actaggacaa
     1441 caatatcacc gaaacgatag tgttcgggat aagaataata gccaaagtct tcgtgtagca
     1501 tataatccat attcaaacgt aaaacctcac cgataatttg agccacatca ttggtaggga
     1561 atcgaatccg tacatagtcg aataacatct ccaatgggtt atcgggattg aagcgttcca
     1621 aagcgttcag taaatcttct ttcacctgtt gagtagggac tgccttgcct gtttcaatat
     1681 ccgataggta aggtctggta atatgagaag caaccgccag tttattttga gaaacgccat
     1741 aagccagtcg cttttctttc aattccttaa tccaaatttt ttcgttttcc attcgcaaac
     1801 ctccaatcta aaaaagatgt aaactatgac ccttgagttt acacctttca tattttccgc
     1861 aatcctttgt accatcaaca ttggttgatg agaattgcct gttttcagtt gtatttgtgc
     1921 ccccctgtta gatagagggg gctaaaggat tggcgtggct aacgccacac caatcaaagc
     1981 aagtctgaac ctgtaccttt cacttcgtgc ggtacaggct cagattgctt tttttgaatg
     2041 acttttccta tttctcctaa gaaatcgtac ctttttggta caaggggtgt ataaaactca
     2101 ctaatcacac tttttcctgt atccacgtaa cctcttccct tgatgggttt taagaaaaat
     2161 tgtttttgtg tatcgcttcc gaacatcata ccaaagccca tttcagacat acgacccaaa
     2221 gccacacgga agttaaagtt gtcccgaatc ccatcactaa aatattttgc gtctggacgt
     2281 tgacaggcaa gaattaaaaa gaagcctgct tgtcgtccta acattacaat ttgtttgagt
     2341 ttatgaataa cggtggtact ttcacgacct agcatatcca tcattgccac atattcatca
     2401 aaaataagaa aattggcaga caaaccaaga taagcataat tttctcctgt tttatagtta
     2461 gggtgttgtt tcatggtttc actgcgatca atcatatctt cgtaaaattg attgatacat
     2521 gccaacatat cttctttacg gtaatagaca ttattcatga ccgcccctaa gtctgccaaa
     2581 tcagcatttt tagggtcaag aatataaagc tttgcatttg tatgcaggag agattcaatc
     2641 aatgtgagaa taaaatagga ttttccacta ccagtgcccc cagcaattaa catgtgaggg
     2701 agagaatcga actcccaata gaccgattcc atcaatttga cacgtccatc ttgagctacc
     2761 acgtcttcaa tgccaattcg attgccaata gtatcataaa gtaggacata ctctacataa
     2821 gaatctttca acgttttttc cactaattca caatacaatc ctgtttctag tttgttttct
     2881 aggtgcaaaa gctgatcttg atatttccct aaggtgattt ctacatgaat atggattagt
     2941 ccatctttca ttcgataata tattttagga aaatggcgga ttcgttgttt ggttttcttg
     3001 ctgggtaaat ctttaaagaa tccctctgat tgagtttctt cggcttcata ccaatgattt
     3061 tctaaaatca tacgagctaa cttttgacgg tgctctaact gttttataga atctatacga
     3121 tagtgataat aaagccagct gataagtccg catacaaaaa gagcagtgag aatactgctc
     3181 caaaagtatg gcgtatgaag ttgatagaaa aatgtatcta actgaactgc ttgccagttc
     3241 accgattgta acgctctcca atgaaaaggg agtaggacga ctagaaaaac taccagcaat
     3301 gaaattagta caaaatgata gaccaagtgc ttatcgttgg ctttgattcg ctctcctctg
     3361 tcatgaagca atggcataaa aatcctcctt tccacctatc aaaataattg tgtattgtag
     3421 atacgagcct gtttatcaaa agttggttga gaaatggctt catatttctg ataagcgatt
     3481 cgcttttcaa agcgtaaatt tttttctttc ggataataat gacgtcgata gagagtgtgt
     3541 cctaacagtt ttttcgcttc ttttgtcaac ggatagagat agcgaaacat gcgtccattg
     3601 attttctcaa tccctttgta ttcgcaaaat gcctgggaaa gccaatgctt tttctctacc
     3661 tgttcaaagc gagcattttc ctctaataaa aggcgagcac ttcgagggtg tatcttttcc
     3721 catgactgct tatcacgata gacacttgtc ttgaaatacc cgcagtaaaa gaagttggac
     3781 gcttgataga catagccaca ttttccctca attccatctg ccagtgtata taaaaaatga
     3841 cagtccgtat gttctttcaa ccacttaatt agagctgaaa gagcttgact gccaaaataa
     3901 ttggtttgat tcatttctgg aagaaaacac atctttccaa tttctaaata atctgtggtt
     3961 tgaagggaac tgtcgggaaa gagtttgcgt atggtttgta ggggttgcgt accccagcct
     4021 aattccacaa cgcctaataa tttttcttct gaaaaaatcc ctagaaagta tttacacaat
     4081 ctagggagta tctttgaata atggtattgc cgaataaaag aaatggcttc atccttcgga
     4141 atttctttca aaatcaatgg tgtgtttgtc ataagcaaca cctatttttc actttcggac
     4201 gcttcttttt tatatttctg atgaggtttt gtctgtcctt ttaatacaag atcgtctgct
     4261 ttcacgtacc aatcaacgtc tgcccctcga aaagtagccg tcgctacggt atctgccaca
     4321 ggattgatta attccacttc tgcgttgtag tcaaattctt tcaaaggaac agaagcgggg
     4381 atactgactt gtatcatgcg tccttgtcct tttgatttta agtcataggt acgttcttta
     4441 atttcttcgg aaaccgtacc atcttcattc tggatacgaa cctcacgacg caaggcggaa
     4501 aatttcaatg tgccaaatgt ctggtctttt tcaatcacaa taccttctgc taatctcata
     4561 ttttttcacc tttcctttct tatgctttca ccatatcgtc tgcgtgcata atgtaattgg
     4621 taaatcctcg attgccaatt ttataacctt ctgcggtaat atgtgggttg atgagtttga
     4681 ctttttcgtc ggattcaaaa tgtttctcgc cagcttcgga gggaagaatg acaacaatat
     4741 catctgctcg ttgaatcgtt gagtataaat tgtaactacg agagagaatg gttggtctgc
     4801 catttattcg acgttgttca atgttccctt caccagcata ttctaattgt ccaaatgttt
     4861 tttccatatt gggaatgaca tatttaagtt ccatattttt acctatcctt tctgttttta
     4921 tgtttgaatg aatcttattt gtgtgatggt tttaaagggg ggagagcgac cttttgattc
     4981 ttgatttttt attttcataa gttcacttcc tttcaaaatt gggtaaaaaa atagacgctc
     5041 cgtactgagc gtccgattcc
//
LOCUS       DACDID010000155         5014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDID010000155 DACDID010000000
VERSION     DACDID010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.160"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..2144
                     /locus_tag="IV236_002347"
     CDS             12..2144
                     /locus_tag="IV236_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8772513.1"
                     /translation="MQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAF
                     GLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWI
                     ADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFH
                     IVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSY
                     LLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSI
                     FLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVN
                     SSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLIS
                     SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNE
                     IFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIV
                     GVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTI
                     IENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLAL
                     ARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILV
                     LHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2113..3528
                     /locus_tag="IV236_002348"
     CDS             2113..3528
                     /locus_tag="IV236_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8772514.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3561..4595
                     /gene="sppA"
                     /locus_tag="IV236_002349"
     CDS             3561..4595
                     /gene="sppA"
                     /locus_tag="IV236_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8772515.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4597..>5014
                     /locus_tag="IV236_002350"
     CDS             4597..>5014
                     /locus_tag="IV236_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8772516.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGD"
BASE COUNT         1810 a          702 c          959 g         1543 t
ORIGIN      
        1 catttccttt tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga
       61 ttttaaaata ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg
      121 atttagaggg tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt
      181 gtatggctat tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa
      241 ttgcacatat attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg
      301 gaaacgtact atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt
      361 ttcaaaaaga atggacaggt attcttttat tacctatgcc tagagaaact tatattccta
      421 ttaaagaaaa agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat
      481 tagtattcca catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat
      541 attattttca aggtattctt gattttttca tccctaatca agcacgttca actttaaata
      601 taatttccat tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta
      661 gttatcttct tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca
      721 aacatgtgtt aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt
      781 ctcgttttct tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat
      841 ttttagatat tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt
      901 tgtttttaat cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga
      961 aaagctatga aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta
     1021 tcatagaaag tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat
     1081 ataaccgtat agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc
     1141 ttgataatat tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt
     1201 tatggctagg ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat
     1261 acaatgcttt actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga
     1321 aaatgcaaac ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag
     1381 aaaaatcgaa ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca
     1441 ctgtaaaaaa cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat
     1501 gtacaattcc ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa
     1561 ctcttgcaaa gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa
     1621 acattaatca cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc
     1681 aagagtcatt tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc
     1741 caccaagttt cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta
     1801 atcaacagcc attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag
     1861 ggcaacgaca acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt
     1921 tagatgaagc aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc
     1981 tcaatttaaa agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat
     2041 gtgatcaaat tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac
     2101 ttcgttttgc cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt
     2161 atgcagaata ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc
     2221 taccgttggg tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc
     2281 tttgctaaaa aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag
     2341 aagttacaag tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt
     2401 gttcataagg acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct
     2461 cagatagaac aagaaaacca acgtattact caacaaataa agatggctca gctatttatt
     2521 gaaagtataa gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat
     2581 caattaaaga gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa
     2641 ttaaatgatc aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa
     2701 attgagagtt cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat
     2761 aatcaatcat taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa
     2821 caactaaata atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc
     2881 ataaatgaac aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct
     2941 aaattagtta aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag
     3001 caagagctag aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa
     3061 caagagaaga accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta
     3121 aaagctcctg tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta
     3181 gccaaaggaa cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt
     3241 acagcacttt tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt
     3301 aaattggatc aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca
     3361 gaaaattcta ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca
     3421 actcaatcgt ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa
     3481 aaaacatatt ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta
     3541 agaatatagg aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg
     3601 gattattgtt actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat
     3661 caaatacaac cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag
     3721 aaacgttaga agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta
     3781 ttgcagatac aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt
     3841 taacacagct aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa
     3901 attcacctgg gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta
     3961 aaaagctaga tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact
     4021 acatttctgc tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg
     4081 gtgtcattat ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg
     4141 cgacgtataa aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg
     4201 aagagaataa agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg
     4261 tagcaaaagg aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt
     4321 atgatggtaa ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg
     4381 cattagattc cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa
     4441 atgacacaac tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc
     4501 taaaggcaac tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata
     4561 ctccaaaacc aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa
     4621 actttgccta attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat
     4681 ttaatttgta ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga
     4741 ctggaatctg acaaatccat tattactttt tatggtatag cgtcattagc tatttattta
     4801 acctattttg tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt
     4861 gggataagag tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt
     4921 gagtgtatat gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct
     4981 gctttcacgc ccaagaaaca acatattggg gatt
//
LOCUS       DACDID010000156         5010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDID010000156 DACDID010000000
VERSION     DACDID010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.161"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            58..1773
                     /locus_tag="IV236_002351"
     CDS             58..1773
                     /locus_tag="IV236_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772517.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1776..3539
                     /locus_tag="IV236_002352"
     CDS             1776..3539
                     /locus_tag="IV236_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772518.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3579..4115)
                     /locus_tag="IV236_002353"
     CDS             complement(3579..4115)
                     /locus_tag="IV236_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8772519.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4438..4860)
                     /gene="mgsA"
                     /locus_tag="IV236_002354"
     CDS             complement(4438..4860)
                     /gene="mgsA"
                     /locus_tag="IV236_002354"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8772520.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1504 a         1008 c          977 g         1521 t
ORIGIN      
        1 caatgtggta gtacttgttt tttcaattgt ggacaaataa ataataggag gaatcatatg
       61 attcagttat tacgttatgc aaaagactat cgtaagcaaa tcattttggg cccgttcttc
      121 aaatttcttg aagctgtctt cgaacttgtc ctgcctttaa tgatggcttc cttgatcgat
      181 aatggactaa aaatgaatga ccgaggaaaa atcattgaaa tgggcttatg gatggtcgct
      241 atgtctgtga ttggtctgat ttgtgctatc atttgccaat actatgcttc agtagcttca
      301 caaggatttg gtacagagtt acgtaatcag ctaatcaaaa aaatcaatac tttctctcac
      361 aacgagttga atcattttgg aacagatacc ttgatcaccc gaatgacaaa cgacatcaat
      421 cagcttcagc tagctttagc aatggtcatt cgtttattga tcagagcacc gtttttaagt
      481 atcgggtcag tggtcatggc ttttgtgatc gactgggaag taggtctgtt tttcttagca
      541 ttattgccta ttttctcaat cattttattt tttatcatca aaaaaaccgt tcctctctac
      601 caaaaagtgc aagaaaaact agatcagctc aatgaacacg ttagtcagaa tcttagcggt
      661 gttcgtgtca tccgcgcatt tgcgcagaca aaaaaagaaa cagagaagtt cgataaaagc
      721 acaaatgatc tagcgaacaa ttatttacgt gtttccaata tctctgccct attatcacct
      781 gctacaacct tgatcatgaa tgtcgggatc atctgtttgc tgacagttgg cgggatcaaa
      841 gtaaatattg gtagcttgca acaaggacaa gttcttgctt tgatcaatta tatgaatcaa
      901 atgcttttgg cactgatcgt tgtttcaaac ctcgtcgtga tcttcacacg tgccgaagct
      961 tcaggaaatc gggtgaaaga ggtgctagac acagaaaatt cgattcttga agctgaggtc
     1021 agtactactc ccgatttttc ttctgaagca attatcgctt ttgaccatgt tgacttccgt
     1081 tatactcctg attctggtct ttctctccaa aatattacat ttcagctgaa gcgaaataca
     1141 gtccttggaa tcattggtcc aaccggaagc gggaaaacga ctttgacaca attgattccg
     1201 cgtttctatg atgtcagttc cggtacagtt ataatggacg gaatggatgt tcgttcttgg
     1261 cagctggatc atctccgtca gcaaattgct cttgttcccc aaacttctgt actatttacc
     1321 gggactattc gtgaaaacct tcaatggggt aagaaagacg ccacagaaaa agaatgttgg
     1381 gaagctctga aaatcgctca ggcagaagaa tttgtccgtc agcttccaaa tggtttagat
     1441 acaaaagtca tggaaaatgg gaaaaatttc tctggcggac aaaaacagcg actgacgatt
     1501 gcaagagctt tgattgccaa gccagcatta ctgattttag atgactcttt aagtgcgttg
     1561 gattatcaaa cagacttgga tttgcgtaag gcgctacaga cttatcttgg cagtacagtg
     1621 attattatct cccaacgtgt ccgctctatt caagaagcac atcatatctt agtgatggat
     1681 caaggtaaga ttgctgctca gggtacccat gaagaattac ttgctcaatc agcagaatat
     1741 caggaaatcg tcgcatcaca ggaggaagaa tagttatgaa agtaaaaaaa ttatccttgc
     1801 agacgctccg ccgattcatt ccttacctta ctgcctatgc aaaagacatt tggctagctg
     1861 tgatactagg aatcatcaat gggattgcaa cagttgtgat gacgttacaa attggtcaaa
     1921 gtatcgatca aatgattgga gaaggacagg tccgcttcaa tcgactgttc catttactta
     1981 ttctattcgg tggtatcgtt ttaatcaacg tagtcagtca atggctcatc caagtgttaa
     2041 gcaatcggtt agcttactta tccgttgcaa aattaagaaa agatgcattt gctcatctga
     2101 atcgattgcc acttcgttat tatgatcaaa actcccacgg aaatatcgtc agtcgattta
     2161 caaatgatat ggacaatatt tccattgcaa tttctgcggc cttcaatcaa ttattttctg
     2221 gagtggcaat catcattctt tcgtttattt ttatgatgcg tctaagtcca cttctgacat
     2281 tggtcgttct ttgttctaca ccaattattt tccttgtgag ttggatcgtt gctcgtgctt
     2341 cacaaacaaa tttcgataga cagcaaagga ttattgggga tatctcgggc tttgtgaccg
     2401 aacgaatcgg taaccagaaa atcgtgaaag cttttcagca agaagaagct agtcaacagc
     2461 aatttgaact gctaaatgct gatctatatg aaaaaggaca acgggcacaa ttttcttctt
     2521 ccttgacaaa cccgtcttct cgttttgtag atcaccttgc ttatttatcg attggtttag
     2581 ttggcggcct attggcattg aagagtggtt cgtcaatcac tgttggtgtg atttccagct
     2641 ttacgatcta ctcaagtcag ttttctaaac catttatcga actttctgga atcacgacac
     2701 aactgcaaac cgctttttct ggtctagaac gagcatttga tttgatcgat cagccagaag
     2761 aaaagcctga tttgccagat gcctatgcct tggaacccaa aaatatcaaa ggaaaagtct
     2821 cttttgatca tgtttctttt tcttatgacc ccaagcagcg tttgatcgaa gatttcaatt
     2881 ttactgctta tccaggtcaa accattgcta tagtaggaaa aacaggggcc ggaaaatcca
     2941 cacttgttaa tttgctgatg cgtttttatg acacaacaaa aggaacaatt acagtcgatg
     3001 accatgacat ccgctctatc caacgtgata cattacgtaa aagttttgga atggtgttac
     3061 aagatacttg gctatttgac agtacattgc gagaaaatct tcgttttggg aatccggaag
     3121 caagtgacgc agaaatcaat gatgccctaa aagcctctta tatgtatgaa tttgtagaac
     3181 gtttgccgga aaaattagat actaagattg gcggtcaagg gttaaagata tcagatggac
     3241 aacgccaact gttgacgatt gctcgaacga tgatcagtaa tccccctatg ttgatcttag
     3301 atgaagctac cagctcagtc gatacattga cagagaaaaa aatccaacaa gccttcttag
     3361 caatgatgga aggaaaaacc agttttgtga ttgcccatcg cctgtccaca atcaaaaatg
     3421 cggaccagat attagtgatg gatcagggag ctattgtcga aatgggcacc catgatgagc
     3481 tgcttcaaaa agatggttat taccgacggc tctatcaagc acaattcaca gcacaataaa
     3541 aacaagcagt cttctgggat gggaagactg cttgtttttt atttagcgtg gatcgctaaa
     3601 cttcttaaaa tttttttgta aggaatacta cgtcctacgt aataaatggc aattgattga
     3661 atcggtaacc atattactgt ttttaacagg cggggcgccc aaacgaccca gctgaacaat
     3721 ggcacatggt acatcaacgc aagccataat ggtgtcaaaa ataagttgat gatcagcgtg
     3781 acactaagcg tagccagaat ggcatttttc caagtgatct cttttcgata gaaaaagaat
     3841 ccgtagattg ctccttcaat aaatgcattc aatgtaaatc caataaagaa tgcagatttc
     3901 gagaagaggg ccattcctac taaatcagct aaaactgcgc ctatccctga ccaaaaagga
     3961 ccaaaaagaa tgcccatgat cacttgtgga ataaatgtga cactgatttt aataaatggg
     4021 gtctcgattg ctaccagtcg ggacaaagtg accatcaatg caatcaataa gcccataata
     4081 gcaatcatac gtgcgtctaa ccggtgtttc ttcattttta gcatctcctt taagagctgc
     4141 tacatcgtat catgcagcga atgcgaaaat aaaaccgcga acattacgtc cttcgtccaa
     4201 gaggcaacat cccatcctct tagcacttaa cgctttattt tctactctgt attatttttg
     4261 ggatcttttc ccacagaaac tatagcatat tttcacattt actgcaaata cgaaaaacta
     4321 atattattgt ttatattatc caatacgttc gtattatatc gcatattatc agtttgttta
     4381 gcttttatag ctacatatag gcgaacatta aacgttattt ttccatacgc ttatccatca
     4441 aaatgaaagt ggatgcttgt ctatttcatg gacgattctt cgaaaatcta ggaagccggc
     4501 ttccagtcct cttagtagca cctcagctgt tccgatattc gtagctaacg gaatctcata
     4561 gacatcagat aaacggataa gtgctgttac atctggttca tggggctgtg cagccagcgg
     4621 gtctcgaaga aaaatcacca aatctaactg atcctccgaa atcatggctc caatttgctg
     4681 atccccgcct aacggtccgg atttaaaccg atggatcggc aagccggttg cttccgtgat
     4741 cctctgccca gtcgtcccgg tagcaaaaag ttcatgtttt tccaatattg ttttatacgc
     4801 tgttgctaat ttcaccatca taggtttttt tcggtcatga gcgatcaaag cgattttcat
     4861 aagagacact ccttttgata ttgttaacat acttatttac tccagtgtat cagtttttat
     4921 ttcttatgga tatttattaa agcttaaaac tagcacattg acaaaaaaaa gaaaaaattc
     4981 ttgttcaaca tgacattgaa gttagttaac
//
LOCUS       DACDID010000157         4985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDID010000157 DACDID010000000
VERSION     DACDID010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.162"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IV236_002355"
     CDS             102..2543
                     /locus_tag="IV236_002355"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8772521.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="IV236_002356"
     CDS             complement(2873..3577)
                     /locus_tag="IV236_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772522.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="IV236_002357"
     CDS             complement(3764..4009)
                     /locus_tag="IV236_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772523.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="IV236_002358"
     CDS             complement(4026..4877)
                     /locus_tag="IV236_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8772524.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
BASE COUNT         1533 a          899 c         1054 g         1499 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgatt
//
LOCUS       DACDID010000158         4856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDID010000158 DACDID010000000
VERSION     DACDID010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.163"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..272)
                     /locus_tag="IV236_002359"
     CDS             complement(12..272)
                     /locus_tag="IV236_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772525.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            347..550
                     /locus_tag="IV236_002360"
     CDS             347..550
                     /locus_tag="IV236_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8772526.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IV236_002361"
     CDS             complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IV236_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8772527.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1179..2078)
                     /locus_tag="IV236_002362"
     CDS             complement(1179..2078)
                     /locus_tag="IV236_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8772528.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2091..2684)
                     /locus_tag="IV236_002363"
     CDS             complement(2091..2684)
                     /locus_tag="IV236_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8772529.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2925..>4856)
                     /locus_tag="IV236_002364"
     CDS             complement(2925..>4856)
                     /locus_tag="IV236_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8772530.1"
                     /translation="VVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADHGKIFKRL
                     WINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIP
                     GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQ
                     EAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTL
                     EAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYV
                     NNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILT
                     QVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKET
                     KPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKV
                     EKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFV
                     SHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTS
                     EACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGF
                     EFSEKKKTNYKKRTRRTTK"
BASE COUNT         1205 a          987 c          848 g         1816 t
ORIGIN      
        1 cagctcttct tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc
       61 gattcaatga ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt
      121 ccatcatcca taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc
      181 gattcatgaa ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg
      241 attttatcaa gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa
      301 aaacagctaa aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt
      361 aaagtttgtg acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca
      421 aaatcttaca gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag
      481 tttcggttct ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca
      541 aaaaaaataa ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct
      601 ggtcaccgtc ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc
      661 cattataaca actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc
      721 gattgattgt ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc
      781 tacatcccgt tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc
      841 acggctttct gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt
      901 cacatacaca cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac
      961 gccgatcaaa ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat
     1021 cacacagtta ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac
     1081 agctagggta aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt
     1141 ggttaagcgt cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc
     1201 atttgttgtg atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt
     1261 ttcattaggt gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg
     1321 ttaactgagc gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc
     1381 atttctcttt cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt
     1441 cgaacacttt tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag
     1501 tcgtagtaat cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt
     1561 tctgcaataa tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt
     1621 aattggtgat agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt
     1681 ttaaacggta cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa
     1741 aaatcggctt ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg
     1801 ggttttctag ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt
     1861 ctaaatgtcg tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat
     1921 aaaaagccgc ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt
     1981 agacgatcca tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct
     2041 aaatttcttg tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag
     2101 ctttgcatgt ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat
     2161 ttacttggat ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca
     2221 tattcaaatt catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata
     2281 gtaataggat ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc
     2341 ttcacgaaat gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca
     2401 tggggaaaag ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa
     2461 gtcgtttcat gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct
     2521 attccatgac catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc
     2581 accaatcttt gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa
     2641 tttccatcgc gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt
     2701 cgttacaaat acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc
     2761 tatcgatcta attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt
     2821 cttttatttg cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt
     2881 tcttgttgtt tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg
     2941 cgtgttcttt tcttatagtt cgtttttttc ttttcagaaa attcaaagcc tgttgtaaag
     3001 tcttctttta aaacgatcgt tgcttcaaac gaacctttct gaccttttag tcctctgact
     3061 gattggcttg ttcggccgtt ttgaatgatc tctttcagtt gtgtggctgt gagtttcttt
     3121 ttggcaatcg ttgtccataa tttaaaatca caggcttcag acgtacatgc cgccacattt
     3181 tgataaagca agacttcttg ttcccgacat tttggacaaa cacctaaatg acgcgttgct
     3241 tcctgttttt cactggctgc tttcacctct tgtaacgttg atccaaaatc tgttgactgg
     3301 atttgtcccg gtacgacttc caacaagtga gagacaaatt tttggatatt agctaagaaa
     3361 gtagcttgtg ttcctttctg ctcaccaatc tttaataagt aactttccca tttggcggtc
     3421 atttcggcac ttgttagcag cggctctttt tccacggctt ggcaaagtaa aatcccttta
     3481 tttgtcacca caagcttatt tttttctacc ttgatatatt ccttttgttt caaagtttca
     3541 ataatatttg ctcgtgttgc ttctgtaccg attccttcta cttctttcaa tatggattgt
     3601 gcttcttcgc tatccacggt ttttccagct gttttcatcg ctgtgatcaa cgtaccttcg
     3661 gtataaggtt gtggtggctt cgtttctttt tcaaggactt gtagatctac cgtcaccgct
     3721 tctccttttg tcactttagg aagtagcggt tcttcttcct tttcttttct gtcgctctct
     3781 ttgccaaaca gctttttcca acctagatct actggtactt ttccgactgt ctttagctga
     3841 agctgctgaa cttgcgtcaa gatcgttgtt tcttcatagg tatacttttc cgcaaacatg
     3901 gccaccgttg tttttaacac ttgttcataa atagcctgct gcagtggact taattgttca
     3961 aaagccgctg ccgtcatgac cgtctttgta ggaatgatcg cgtaatgttc ctggactttc
     4021 ttattattca catatcgact attcggtgta tgaataggag taggaatggc ttcggccttt
     4081 aaaaagtgtt tgtattcgtc taagtgatcc aacagatacg catactcccc ttctgtaata
     4141 taaggggtat ctgttcgtgg gtagcttaga tatttccctt catacaaccc ttgcatggct
     4201 tccaaagtat ctttggcact agctttcatg agctgattca ttttagattg aaggctggat
     4261 agagaaaaaa gacgtggact tgccgcactt tttttctcgg tttcaacttg gtggatcgta
     4321 cctggctgtt ttcccaattg aacccccttt tcttttaaat aatcttcaaa tgcttcttgc
     4381 gtcgcttggg tttcattcgg atcaagcttc gcatcaaatg cgccattttg agcgattact
     4441 tgggcttttc cttcaaaata tggttctttt tggaagtttc taatcttttc ctgtaattga
     4501 taaatcatgt aaagcgttgg ggtttggact ctgcctaagg aaaaacttcc tggtatgcct
     4561 ttttgctgca agagtaaact atacagcggg ctaccgttca tgccgatcaa ccaatcggcg
     4621 atctggcgtg cttgtgcttc ttcaaatttt gggtaggtct cttctgcatc tcgtaactgc
     4681 tggaatccct gataaatggc ttctttttct aaggagttga tccaaagtct tttaaatatc
     4741 tttccatgat cggcgcctgc ttgttgaatg atcgtccaag cgatcagttc cccttcacga
     4801 tcgctatctg tcgcaataat aatggtgtcg gccgattgaa gttgctgttt caccac
//
LOCUS       DACDID010000159         4815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDID010000159 DACDID010000000
VERSION     DACDID010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.164"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1932
                     /locus_tag="IV236_002365"
     CDS             <1..1932
                     /locus_tag="IV236_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8772531.1"
                     /translation="VVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADQGKTFKRL
                     WINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIP
                     GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQ
                     EAFEAYLKEKGVQVGKQPGTILEVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTL
                     EAMQGLYEGKYLSYPRTDTPYITEGEHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYV
                     NNKKVQEHYAIIPTKTVMTEATYEKLTPLQQAIYDQVLKTTVAMFAEKYTYEETTILT
                     QVQQLQLKAVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGETVTVDLQIFEKET
                     KPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKV
                     EKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLTKIGEQKGTQATFLANIQKFV
                     SHLLEVVPGQIQATDFGATLQEVKVASEKQEAARHLGSCPKCQEKEVILYQKVATCTS
                     EACDFKLWVTIAKKKLTATQLKEIIQNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGF
                     EFSEKKKTTYKRKVRRKTS"
     gene            2178..2771
                     /locus_tag="IV236_002366"
     CDS             2178..2771
                     /locus_tag="IV236_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8772532.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            2787..3686
                     /locus_tag="IV236_002367"
     CDS             2787..3686
                     /locus_tag="IV236_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8772533.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            3689..4174
                     /gene="ssb"
                     /locus_tag="IV236_002368"
     CDS             3689..4174
                     /gene="ssb"
                     /locus_tag="IV236_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8772534.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            4540..4800
                     /locus_tag="IV236_002369"
     CDS             4540..4800
                     /locus_tag="IV236_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772535.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1768 a          835 c          984 g         1228 t
ORIGIN      
        1 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
       61 gaaggggaac tgatcgcttg gacgatcatt caacaagcag gcgctgatca aggaaaaacc
      121 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccagcaa
      181 ttacgagacg cagaagagac ctatcccaaa tttgaagaag cacaagcacg ccagatcgct
      241 gattggttga tcggcatgaa cggcagccca ctgtatagtt tactcttgca gcaaaagggc
      301 ataccaggaa gtttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcag
      361 ctgcaggaaa agattagaaa cttccaaaaa gagccttatt ttgaagggaa agcccaagta
      421 accgctcaaa atggcatatt tgatgcgaag cttgatccca atgaaaccca agcgacgcaa
      481 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaag taggaaagca gccaggcacg
      541 attcttgaag tcgaaaccga gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc
      601 agccttcagt ctaaaatgaa tcagctcatg aaagctagtg ccaaagatac tttggaagcc
      661 atgcaagggt tgtatgaagg gaaatatcta agctatccac gaacagacac cccttatatt
      721 acagaaggag agcatgcgta tctgttggat catttagatg aatacaagca ttttttaaag
      781 gctgaatcat tccctactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
      841 gtccaggaac attacgcgat cattcctaca aaaacagtta tgacagaagc aacgtatgaa
      901 aagctgacac ctttacaaca agcgatttat gatcaagttt taaagacgac ggtggccatg
      961 tttgcggaaa aatataccta tgaagaaact acgattttga cacaagtcca gcagcttcag
     1021 ctaaaggcag tcggaaaagt accagtagat ctaggttgga aaaagctatt tggcaaagag
     1081 agcgacagaa aagagaagga agaagaaccg ctacttccta aagtaacaaa aggagaaacg
     1141 gtgacggtag atctacaaat ctttgaaaaa gaaaccaagc caccacaacc ttacacggaa
     1201 ggtacactaa tcaccgccat gaagacagct ggtaaaaccg tggatagtga agaagcacaa
     1261 tccatattga aagaagtgga gggaattggt acagaagcga cacgagcaaa tattattgaa
     1321 accttgaagc aaaaggaata tatcaaggta gagaaaaata aacttgtggt gacgaataaa
     1381 ggaattttgc tttgtcaggc tgttgaaaaa gaaccgctgt taacaagtgc cgaaatgacc
     1441 gccaaatggg aaagctatct tacaaagatt ggtgagcaaa aagggacaca agctactttt
     1501 ctagcaaata tccaaaaatt tgtttcccac ttgttggaag tcgtcccagg acaaatccaa
     1561 gccactgatt ttggagcaac gttacaagag gtgaaagtag ccagtgaaaa gcaagaagcg
     1621 gcgcgtcatt taggatcttg tccaaagtgc caggaaaaag aagtcatact ttatcagaag
     1681 gtggcgactt gcacgtctga agcctgtgat tttaaattgt gggtaacgat tgccaaaaag
     1741 aaactcacag cgacacaact gaaagagatc attcagaacg gccgaacaag tcaaccagtc
     1801 agaggactaa aaggtcagaa aggttcgttt gaagcaacga tcgttttaaa agaagacttt
     1861 acaacaggat ttgagttttc tgaaaagaag aagaccacct acaaaagaaa agtgagacgt
     1921 aaaacgagtt agaattgacg gagggaaaaa cgatgagaaa tacaacgcat aaagaacaag
     1981 aaatcgaaaa tcaaaaagcc aaaatacgtt cagagcagga gaaaatgcgt gccagcaaac
     2041 gaagactaca gcaattgaag aatcaacaac ggattcaaaa agaacttgaa ttagatcgat
     2101 aaatcctcct gttgttaata ttaaactaac atgttatagt tatgttagtt taatattaac
     2161 attcgagagg gaaagcgatg aaagataaag tcaagtgcat ttttgagaaa tacaatggta
     2221 atctatcctt gaaggatgct agaaatgaag ggttatcacc tatgacgtta gatcgtttag
     2281 ccgaaaagga caaaatagaa aaaatagcat caggttttta taccttaaaa gatagttttg
     2341 tggatgaatt atatctcgct caaagtatct atactagagg aatcttctct cacgagacag
     2401 cgcttgatct ttttcaagta ggaacatata tccctaaaaa aattaatatg atgtttccta
     2461 aaggatacaa tgtttcgaaa gagaatttag atcagtatgc cattcaaccg cattacacga
     2521 ataaagaaaa ctttgaactg ggaatcacag aggcagaaag cttttacggc aataaaattt
     2581 tcgtctatga tttagagcga acgttatgtg atatgtggaa tcccagatat aaagcaagcg
     2641 tagaagtaaa acaagaagca ttaaaagaat atatgacaag taccagcaga aatacaaata
     2701 agctaagaag atacatgaat attttaaaat caccaaaaga aatgactttg tatatgttgc
     2761 cactttattg agaggagtaa aggtgaatga caccgacaca atttaaagcg aaaacaagaa
     2821 atgttgcagc tgagaaacat ttaaacccac aaattgttca aagatattat atgatggaaa
     2881 aattattaga atgtattgtt gaatcgcctt atcatgatga ttttgtatta aaagggggat
     2941 ttctgatcgg atcaaaatac gggttagaaa accgttcaac cgttgatatt gatacgactt
     3001 atcgaaattc gaaactaaca aaagaaaaat tagaaactgt tttttctgaa ttaacagctg
     3061 ctcctacaaa agatggcatt cggtttgaat taaagggctt aacagagacg agggaagccg
     3121 attattaccc aggttttcaa gcaaagttta ttgcttttct tgaaaatgca cgtgtacctt
     3181 ttaaattaga tattacttca ggtgattcta ttattccagc agcattgata tatgaacata
     3241 aattgatgtt tgaagataaa acaattcata ttccagctta tcctacggaa caaattttag
     3301 ctgaaaaatt acatgccacg tttagtttcg gaatagataa ttcacgtatg aaagatttct
     3361 atgatctgta tatgatccct aaactagaac gtatcgatga gataaatttg tataagagtg
     3421 ttcaaggaac atttaaggaa agaaataatc aatcagtgtt taagaatttg tataatcagg
     3481 aaatgcctca attgatgaag aatgagggat tgagggagaa atggaaaaga tatcagcaag
     3541 aaaactattt tgccaaagat atttctttcg aatctacctt aacttctgtt gattcgctga
     3601 tgcaatcaat tgtcgagcaa gaggaagtag aacgaaaaca aaattttaag agatcacaac
     3661 aaatgaatca agaaatggag agataaagat gatcaataat gtaactttag taggtcgatt
     3721 aaccaaagat cccgatttac gttacaccgc aagtggcact gcagtgacca cttttacctt
     3781 agccgttaac cgtaatttta ctaatcaaag tggcaatcga gaagctgact ttatcaattg
     3841 tgtgatttgg cgcaaaccag ctgaaacgat agcgacatta gctaaaaaag gcagcttgat
     3901 cggcgtcgtc ggacggattc aaacgcggac ttatgacaac cagcaaggac agcgtgtcta
     3961 tgtgactgaa gttgtggccg ataatttcca actattagaa agtaaagcag cgacagaaag
     4021 ccgtatgcac gctgatcaat cgtccagcag tccaagccct accacctttg aacaacggga
     4081 tacagcgacg cctaacaaca atggattgaa cgcttcacag aatccatttg gtggacaatc
     4141 aatcgatatt agcgatgatg atctaccgtt ctaagtagca ttttctattg gttgcatttg
     4201 ctacggttta tgttataatt tagacaacaa aaagaatttt caatctataa ccaaaaacca
     4261 ctagctggcg ggctagtggc tttcttttac gtttctatta taagaattaa cgggtaggct
     4321 tactgtcttt cttggcaaat ttacaatcaa gccatttgca gatatagtgc gcggctactt
     4381 ctgccatgat tgcaaaaaca aaatcaaaaa gaattttcaa acctataacc tcctcccctt
     4441 gccagattag gccggggtag cagacagcaa gactattata gcataattga aatatccaaa
     4501 tgattggatt tttgctataa ttttagcgag gtgattgtga tgaacaaata tgttggtttg
     4561 cttgataaaa tccgagtaat aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg
     4621 attcatgaat cgattaattg tgtagttgca gatattgaaa ttatcgataa attgctaatt
     4681 atggatgatg gaaaatacaa tattgccgtc acaggtcact tcaataaacg aaatcagcta
     4741 gttattgcta gcatgtatgt tagaaatccc gatcatttta cgcgatcgat gggcatataa
     4801 ataaaagaag agttg
//
LOCUS       DACDID010000160         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDID010000160 DACDID010000000
VERSION     DACDID010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.165"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IV236_002370"
     CDS             564..989
                     /locus_tag="IV236_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772536.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IV236_002371"
     CDS             2141..3118
                     /locus_tag="IV236_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8772537.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IV236_002372"
     CDS             3128..3625
                     /locus_tag="IV236_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772538.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IV236_002373"
     CDS             4001..4282
                     /locus_tag="IV236_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772539.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IV236_002374"
     CDS             4284..4700
                     /locus_tag="IV236_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772540.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDID010000161         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDID010000161 DACDID010000000
VERSION     DACDID010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.166"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IV236_002375"
                     /pseudo
     CDS             106..357
                     /locus_tag="IV236_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IV236_002376"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IV236_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8772541.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IV236_002377"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IV236_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IV236_002378"
     CDS             1786..1869
                     /locus_tag="IV236_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8772542.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV236_002379"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV236_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8772543.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IV236_002380"
     CDS             complement(2994..3857)
                     /locus_tag="IV236_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8772544.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IV236_002381"
     CDS             complement(3859..4131)
                     /locus_tag="IV236_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8772545.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IV236_002382"
     CDS             complement(4148..4363)
                     /locus_tag="IV236_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8772546.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IV236_002383"
     CDS             complement(4455..>4710)
                     /locus_tag="IV236_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8772547.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDID010000162         4667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDID010000162 DACDID010000000
VERSION     DACDID010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.167"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IV236_002384"
     CDS             complement(26..466)
                     /locus_tag="IV236_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8772548.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IV236_002385"
     CDS             626..1132
                     /locus_tag="IV236_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8772549.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IV236_002386"
     CDS             1300..3213
                     /locus_tag="IV236_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8772550.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IV236_002387"
     CDS             3218..4219
                     /locus_tag="IV236_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8772551.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IV236_002388"
     CDS             complement(4421..4627)
                     /locus_tag="IV236_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8772552.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1707 a          718 c          958 g         1284 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagat
//
LOCUS       DACDID010000163         4661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDID010000163 DACDID010000000
VERSION     DACDID010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.168"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IV236_002389"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IV236_002389"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8772553.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IV236_002390"
     CDS             2714..2938
                     /locus_tag="IV236_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772554.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="IV236_002391"
     CDS             2951..3451
                     /locus_tag="IV236_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8772555.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..>4661
                     /locus_tag="IV236_002392"
     CDS             3548..>4661
                     /locus_tag="IV236_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8772556.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKL"
BASE COUNT         1674 a          691 c          996 g         1300 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg a
//
LOCUS       DACDID010000164         4637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDID010000164 DACDID010000000
VERSION     DACDID010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.169"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1143)
                     /gene="nagA"
                     /locus_tag="IV236_002393"
     CDS             complement(<1..1143)
                     /gene="nagA"
                     /locus_tag="IV236_002393"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8772557.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFH"
     gene            complement(1219..2055)
                     /locus_tag="IV236_002394"
     CDS             complement(1219..2055)
                     /locus_tag="IV236_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772558.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2239..3456)
                     /locus_tag="IV236_002395"
     CDS             complement(2239..3456)
                     /locus_tag="IV236_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8772559.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3839..3922
                     /locus_tag="IV236_002396"
     tRNA            3839..3922
                     /locus_tag="IV236_002396"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3873..3875,aa:Leu,seq:gag)
     gene            complement(4162..>4637)
                     /locus_tag="IV236_002397"
     CDS             complement(4162..>4637)
                     /locus_tag="IV236_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772560.1"
                     /translation="NLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSL
                     FYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWA
                     SGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1383 a          982 c          753 g         1519 t
ORIGIN      
        1 gtggaaacgt tccacgccat ctagataagt tgcttccaat gtcatatcag ggttcaatac
       61 gataaagtct gcatcgcgtc cttgagcgat ttttccacat gtatcatcga ttttacagct
      121 gattgctgga atcagactag ccatcatgac tgcttgttct ggtgttgcta atccccagtc
      181 tacaacattt ttaattgctt ctttcaattt caatatactt ccagctaagt tgccagattc
      241 tagacgtgct gttccttctt ttactactac tggaaattcg cctaaattgt aatttccatc
      301 aggcatccca ccggccatca tacagtctgt aattaatgcg acatgatcat gtcctgcttt
      361 ttccattagg attcccgcag catttggatg tacatgatgg ccatcacaaa tcaattcaga
      421 aaagacatgt tctaaagata ataacgcacc caccatacct ggttcgcgat gattcaatcc
      481 acgcatacca ttgaaagcat ggacaaatac acttgcgcct gcttcaacag ctttttgcgc
      541 ttcttctagt gtagcattac tgtgtcctag agaaacaact actccttctt cagtcacttg
      601 tttgacaaac tcttctacac cttgacgttc cggtgctaaa gcaatttttt tgattaaacc
      661 acctgaagct tcttgccatt catggaaagt atccaaatca ggatccccga aataacttgg
      721 gttttgtgcc cctttgtgtt cttctgtgaa gaaaggacct tcaaaataga ttcctttgat
      781 tttagctcct ggtgcttcct gatacatttt gccgattgtc tcagctacat cttttaaacg
      841 ttctttgctt gacgtcaatg ttgttggtaa aaatgaagtc actccacaag ataacagacc
      901 ttcagacatc actttgatgc cttctgcatc attatccatt acatcatgat tcatatagcc
      961 gtgaatatgt gtatctacca aaccaggagc aatccatttg ccgctttggt caatgatgtt
     1021 gacttctccc tcaggtagtt ctttggtgta ttcaccaaaa agtccatcgg taatttcaag
     1081 atagcctgca tgtttgacac ctgacgctaa aaagaatttg tcagcataga taaaagttct
     1141 cattctattc tctccctttg tttttctacc tctaaagtac tcttatttag agaagaagtc
     1201 aagaatggtc tacaccattt acaaattttt tgaaatttgt ttcacagctt gtgctaagcc
     1261 ttcatagaat gctgctcttt ctggttcttc tttacgcttg tatattaagc ttttaagcac
     1321 aaataaatca ttgaggtaat agtagctatt cttctcttga gcccaataga ttccttgatt
     1381 cacctgttct aacgcttcgt cgatttcatt ttgatggaat aagaaatttc cataattata
     1441 aaaaatttgc gaaaattcac tttttgatcg ttcatcagct gattgatgca gcagttggat
     1501 gcttcgactc aaatagtagc gtgcttcagc cgtttttcct gccacaccat atatttttcc
     1561 gatacaacta atcagttgta tttctgtgtc agaaacatgg attctttctt tttggctaga
     1621 gtagcttaat gcttctttca aataataaag ggactgagag atattttgac gcaacgtgaa
     1681 ttcgcaaaca ccgagataat aataatagcg ttgaaagtca gttgcttgat acagattttc
     1741 agaaatatca gaagatttta gtacttgttc taacttccga taatcacgtt tttggaaaca
     1801 actatccaac agttcaaata atttatcgtt tgtccgagta gtttttcgag agagattcat
     1861 cacatgatca atcgttaccc ctagccgttg acaaatctgc atcatcacca atacattcgg
     1921 cagttcctca ttattttcga tccgacttaa tacactttgt gaacaaatat catgcgaaag
     1981 cattttttgc gtcatttttt gttcttttcg aatctcttta atgatcgttc caaaggactc
     2041 gattaacgtt tccataagta tcctccccaa aatatgcgta ttcgcattct attcgtattt
     2101 tactttagga agtcgcaaac ttcaagaaaa gacttgcaac taaataatat actttagtta
     2161 tttaagaaaa ctccactaga aagagagcct cagtgtgttt gtctgtggcc ctcctactga
     2221 aaaatttggt ttttaaaatc atttttggcg ataggttgct aggaattctc tcattttctt
     2281 agctgtttgt ttcaaatcat ccattttagg aagataaacg attcgaaaat gatctggctg
     2341 gttccaatta aatccaccac catgtacaag tagaatatga tgttcatgta aaaagtccaa
     2401 aacgaatttt tcatcatcca aaatattgaa tcgctttgta tcaatttttg ggaatatata
     2461 aaaagcagct tttggtttta cggctgaaag accaggaata tcattgattg cattatagat
     2521 atattctctt tgttcataga tccttccgcc tggcaggagc agctcatcga cactttgata
     2581 accgcctagc gcggtttgga taatttgctg agacaataca ttggaacaca aacgcatgga
     2641 ggacaacata ttcagccctt ctatatagcc tttgacgtgt gacttatctc cacttaggac
     2701 catccagcct actcgaaatc ctgctacacg atgagatttg gatagaccgt tcagcgtaac
     2761 aacgaacaaa tcaggcgcta atgtagcaat cggaatatgt tctagaccat ccatcactaa
     2821 gcgatcatag atttcgtcgg aataaataat caggttattt tgtcgagcta cttcaacgat
     2881 ttcttctaat aattcttttg gataaagtgc cccagtagga ttattaggat taatgataac
     2941 aatagctttt gtccgactcg ttatttttga tttgatatca tcaatatcag gataccattc
     3001 tgcctcttca tcgcaaatat aatgaacagg tgtgcctcca gctaatgcaa tagaagccgt
     3061 ccacaacgga tagtccggca ttggaaccag tacttcatct ccattgtcca gcaacccctg
     3121 catacacatc gtgatcaatt cgctcactcc gttgccggtg tagatatcat tgatcgtcac
     3181 attcggaaaa tgtttcagtt ggcaatattg ctcaattgct tttctagcag aaaagatccc
     3241 cttggaatct gagtagcctt ctgagtcacg ggcattcatg atcaaatcac gaatgacttc
     3301 gtttggtgct tcaaatccaa aaggtgcggg atttcccgtg ttcaatttta aaatactaat
     3361 gccctcttct tgcatccgct ccgcttcttc tagaacggga ccgcgaacat cgtaactgac
     3421 accttctaat ttgttggatt tttcaaattc tctcatcact tagccaatcc ctcatttcaa
     3481 aaagttagaa ctttccaaat tttttgttat ttactatttc aatatcatac tccatcctag
     3541 aaaattttgc cacttattta ggaaaaacgt aagtattaaa aaaacacaaa caagcgttga
     3601 gaacttaatt gtcatgatag tcattccata gattcgctgc ccaatccatt gcaattccac
     3661 aaatgattcc tagcataaaa ccaagcaaaa aacgtccgaa tgcaagtcca atcaatacac
     3721 ctaagatcat tcccagtcct gaaaattctc cgtcatattt ctccatctta ataagtcctc
     3781 ccatttttga gcttgacaac tatgtaaaac ctcgctatca ttagtgtgtt attattacgc
     3841 agatgtgttg gaattggcag acaagcatga ttgagggtca tgtgagcgtt agctcgtgtg
     3901 ggttcgagtc ccatcatctg cactacaact cgtgaagtta attttgcttc acgagttttt
     3961 ttgtttgaac gtttctttat gccattatat acatatcact gtatccttta caaaaagaac
     4021 gtacaatcat gtaaacagcc tgaggtgagt atatgaaggt cggaaacaag ctactcgtag
     4081 aaacttgcaa gtgttaaaac aattaatcga aaaacgagta aaatccctca cctaatacta
     4141 aagcgaggga ttttttgtat attatctgtt tatcaccgtt attataaagg tacaaatacc
     4201 aagcacggtt aatccaaatt gcataaattc atttttttgt acactcatgc ttttcattag
     4261 ctctttaact gccattagta ctaagatggc gataacaaca taaagtatat agtacgtccg
     4321 cattccggaa gcccatactg tgggagaaaa tcccataatt aaccttgaac aaaatcccat
     4381 tatcatcaaa ataactaaaa aaatagaagt tttcttattg tcaaaactta aataaattcc
     4441 aacaattaaa catagtaata atgccaagac tactaaagta gctatccaag tacctggata
     4501 aaaaagtgac agtttcgttc ctaactctgt aaagagatta ttcaggttac tactactcca
     4561 aatcattgcc cttttatttc cattgacgta agtaaagctt tgacccattg tattaccaaa
     4621 ataaataatc aggttta
//
LOCUS       DACDID010000165         4631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDID010000165 DACDID010000000
VERSION     DACDID010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.170"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(120..1094)
                     /gene="lacD"
                     /locus_tag="IV236_002398"
     CDS             complement(120..1094)
                     /gene="lacD"
                     /locus_tag="IV236_002398"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8772561.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1091..1549)
                     /locus_tag="IV236_002399"
     CDS             complement(1091..1549)
                     /locus_tag="IV236_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8772562.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1546..3009)
                     /locus_tag="IV236_002400"
     CDS             complement(1546..3009)
                     /locus_tag="IV236_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772563.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2991..3923)
                     /gene="pfkB"
                     /locus_tag="IV236_002401"
     CDS             complement(2991..3923)
                     /gene="pfkB"
                     /locus_tag="IV236_002401"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8772564.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4065..>4631
                     /locus_tag="IV236_002402"
     CDS             4065..>4631
                     /locus_tag="IV236_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8772565.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         1386 a         1032 c          733 g         1480 t
ORIGIN      
        1 tgttattatt agttttagat aacttttgca gttttatact ttttttctat aaaatttgac
       61 aaacattttt ttaaataact aattattttt tgaatctgtc actcagacaa tcctttctct
      121 tacttgttaa cataaataga agttgctgtt tcttctaata gtgtacttaa ttctttaata
      181 ttttgttttc cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa
      241 ggtgctactc ccttcgccca tgttgctcgc ccacataaga caccattgaa tgtcgaacct
      301 gattgttttg caaaacgcaa tgtatcttga aatagtgcag cgctgactcc cgcacttaaa
      361 aaaataaacg gttgttcagt cactttcgat tgttcataga aatacgcgga tgcctctgct
      421 tgactataac aagtatcttg cccatatcct tccacaaatc tcatattcac tggtacttct
      481 actttcaata catccacttg ataacgttct tttgaaaatt ctcgcatggc ttcaatcact
      541 ttatgcggtt ttacttttgc atagtcaaga gaagacggat cgccaatagt tgcatcgtaa
      601 gaaaggagct ctaaaaagaa aggtatgcct tctgcttcgc attctgaacc aatccgttct
      661 ataactattt gtttttgcac attaatctct actggttcat caacgtcata gtacaataaa
      721 aatttacaag catccgcacc cacttctttt aaccttttca ctgaccaact attcagtaaa
      781 tctggtagtc ggcctggcat tttcgcatca tagccggttt tttcataagc tagcaacaaa
      841 ccacattctt tctctcgaag cgctgctgca ggcagaccat attctggatc gagtaaaata
      901 gcggaagcat acggagtcag gtactgagaa accatctgtt taaattggat gatatcttct
      961 gatgtagctt ctttatattg gttgatcatt ttcttcaaag caccacgttg gtcgatagct
     1021 agcgcgctaa taattccagc ttcatccgtc aatcgcttca aacgttcctg tttttctata
     1081 ctcattttct tcatatatct tcacctattt ctttagttaa tattgtagct atttctattt
     1141 ttgattttgc agctctcaac tgttctgtaa cctcttttcg catcaatgat cgtgcaacga
     1201 gagacaataa tttcaaatga gtcgttcctg cttctgaatc tgggataaat aacgcaaaaa
     1261 taaaatcaac tgactgtcta tctaaactgt tccattcaat tccttttttc agtgtgacga
     1321 taatcaaact aggttcattg attgtttgag acttcgcatg agggatagca aaacctgaca
     1381 ttattcctgt agtaccttct ttttctcttt ccagaagttt ttgatatact tctttttcgt
     1441 catgagcgat tcctaaacag aatgtttgct ttgctaaaaa cttaaatgct tcttcttgat
     1501 tatctaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg ataaacctcc
     1561 tgctcttcct tcttcgataa ctgtacgatt tgttgcttta ttatccctct tcttgtcttt
     1621 atcagttact cttttctttt ggatcaaacc tagtatcact ccaccaacga tcgaaccaat
     1681 aagaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaacacta aaaaaccgcc
     1741 atgtggggct ggtacttgga caccaaacag ataggtcaag acagcagcaa ttgatgagcc
     1801 taacatcatc gttggtaata ctcggatcgg gtctttcgca gcaaacggaa tagctccttc
     1861 cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat cattagtatc
     1921 aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg caatgcaagc
     1981 tgctgacgca ccagccataa aagtcgtgtt tccttgtgtc aaaagagctg ttcctgttac
     2041 atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatacagc caacaatcac
     2101 gccaagaaca agtggattcg aatattcaaa tccagctaaa aagctcatca ttccttcatt
     2161 tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg ctacaccaat
     2221 aactggatat aagacgattg ccttcaagcc atctagttgt ttaggtaaaa ttatcagtag
     2281 cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatac ctcctaaaaa
     2341 tccagtaccg ccattcatgg ctacgattcc accaacaaat ccaacgacta accctggacg
     2401 tttcgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc ccattgctgc
     2461 atcgtcaact gcttttacca ttgccgctgt agcattgtat tgtgcatgat ctggatcaga
     2521 agaatagatt ccccaaacgg caaatgaaat agcaatcaat actccgccag ctaccacgaa
     2581 aggcaacata tgagagacac cgttcatcaa atttttatag atgctacgac caaaattagc
     2641 accacttatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta agacagtacc
     2701 ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg tactgacaga
     2761 aacatcaatg actcgttttc ccacaaatct ttctgaatgg acatctttgt ctgcggcaat
     2821 aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattctcga tcccaatttg
     2881 tccatgggtc tccactttga tcgtgatgcc ttttttccta gctgcttgct ctaatgcctc
     2941 ttgcgccatg taggtgtgcg caattcctgt cggacaaccg gttgctgcaa ttagttgata
     3001 ttttcccatc tttcgaacct ctcttccttg attttgattt gtttacttaa ttcctccaca
     3061 tccaaaaaat cagtcagtcc tttacgaaaa gcagtagagc tgcctgctgc tatgctttta
     3121 cgtaacgttt catcaagcga tcgtcgattc atgtatcctg ctaaaaaagt gcctaacatg
     3181 gcatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc tcgaatcacc
     3241 ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac attttctgcg
     3301 cctgactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac catttttcgt
     3361 ccgaaccaag aagaaagttc ttcctcattt ggtttcagca aaaacggtcg atagggtaaa
     3421 caatccatga cttcttgatc actactatca ataatcaaac aaatccgact gatttctatt
     3481 aaaacctttg gagacagccc tcttggtaaa ctcccagaaa cacaaagata atcccctgct
     3541 tgtaatgctc ttatttgatt taaaaaatta tgaacagctg tctgcggtat ttcaggtcct
     3601 tgattcacta atttgtattc ttctcccgtt tgattgactt gtgtaaatac attgatacgc
     3661 gtcataccag gcacttcaat aaactctgtt tggatttgtt tctgctgtaa atagtcttct
     3721 atatactttc ctgtgaaccc agcagaaaaa cctaatgccg tacttgggaa atctagcatc
     3781 tttaatacta gtgacacatt cacccctttt ccgtttgcct gaatgtcatc ctcttttgtg
     3841 cggtttacca taaaaggatg catctcatct gtttcaataa atagatcaat tgccaaattc
     3901 atcgtgcatg tgtagataga cataaccaac gacctccttg attctattat gaaatacgcg
     3961 aaatagaaaa tgttttctta atcgtcccaa cacaaagaaa acctttacat ttataaactc
     4021 gtcgtgcttt ttttaactaa cttttggtga aagaaggaaa gaatatgaat cacaaattaa
     4081 gtgaaacaga gcagtacctc tggaacttta tcgaacacct catcctagag atccctaact
     4141 attccatcgt gaaactaagt gaacaagcaa atgtctcaac agccaccatt gtgcggacaa
     4201 tgaaaaagaa aggctatgaa gggtttactt cgtttaagca tcatctaaaa gaaggagaaa
     4261 acaagaacat caatttttca tttttagata aagtagacaa aggaatccgc cgagccattc
     4321 taaaaaatga acaagaagtg gtacgtacaa tcaatatgtt ggaaatcggg aacattgaag
     4381 atgcaataca aagaatcaaa tttgctgacc gggtttttgt ctttgcccga ggattttcag
     4441 aaatgattgg ccaagaaatg ctggtaaaac ttcaattaac gggaaaaaat tgtcagatgc
     4501 atactgatcc agaaattatc aaaagcatca gtcggaaact aactaagaaa gacagcgtta
     4561 tttttgtttc tttgaacggt gagaaaacaa ataaaaacat caaaaataag atttatgtct
     4621 ttatttttca a
//
LOCUS       DACDID010000166         4610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDID010000166 DACDID010000000
VERSION     DACDID010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.171"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IV236_002403"
     CDS             complement(85..339)
                     /locus_tag="IV236_002403"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8772566.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IV236_002404"
     CDS             complement(450..704)
                     /locus_tag="IV236_002404"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8772567.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IV236_002405"
     CDS             complement(897..1172)
                     /locus_tag="IV236_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8772568.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IV236_002406"
     CDS             complement(1144..2097)
                     /locus_tag="IV236_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8772569.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="IV236_002407"
     CDS             2709..4202
                     /locus_tag="IV236_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8772570.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="IV236_002408"
     CDS             4337..>4610
                     /locus_tag="IV236_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8772571.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DACDID010000167         4556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDID010000167 DACDID010000000
VERSION     DACDID010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.172"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..718)
                     /locus_tag="IV236_002409"
     CDS             complement(107..718)
                     /locus_tag="IV236_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8772572.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(841..966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1048..1764)
                     /locus_tag="IV236_002410"
     CDS             complement(1048..1764)
                     /locus_tag="IV236_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8772573.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1770..2339)
                     /gene="scpB"
                     /locus_tag="IV236_002411"
     CDS             complement(1770..2339)
                     /gene="scpB"
                     /locus_tag="IV236_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8772574.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2323..3120)
                     /locus_tag="IV236_002412"
     CDS             complement(2323..3120)
                     /locus_tag="IV236_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8772575.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3090..3461)
                     /locus_tag="IV236_002413"
     CDS             complement(3090..3461)
                     /locus_tag="IV236_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8772576.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3480..4367)
                     /gene="xerD"
                     /locus_tag="IV236_002414"
     CDS             complement(3480..4367)
                     /gene="xerD"
                     /locus_tag="IV236_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8772577.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1249 a          990 c          790 g         1527 t
ORIGIN      
        1 aatcaaaaaa atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt
       61 tttttccaaa aaaaaagacc aagaaatttc ttgatctttt tgatatttat ttgactgctg
      121 tatgacgttg ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca
      181 gcactaaaaa gacagcactc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa
      241 tcagtacata gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca
      301 ttaagtaaag aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga
      361 tggttcctgt aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa
      421 agaaataata aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta
      481 ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg
      541 ttgcaactcc ggccgctgga ccgaataaga acatgctgat caataccgag atatcactaa
      601 aatcgatctt caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta
      661 atgcgatcgc agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt
      721 gaaatcctcc tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa
      781 aaactctatt ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag
      841 ctctatcttc tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt
      901 ttttgcaaaa taggtcgtgg actataacca ccggtcggga atttcaccct gcccctgaag
      961 acgaacctta tataattttt gtttacatat tcattataca tatctattca aaaaacgcaa
     1021 catctaacaa ttataaaaag taagtgttca ttttgagtgg ttcagcaaac tgctgacttc
     1081 ttttttgttc agttcacggt aatccccagg acgtaggcct tgtaaagtca gatctccgta
     1141 tttttcacgt tttagttttt gcacaggaag accaactgct tgaagcatat ttttcacttg
     1201 atgattacgt ccttcgtgga tcgtcaattc cacgatactg tggtttgtct taggatccac
     1261 tgataaaatt tggaatttag ctggagatgt ttttcgtcct tcgatcttta ttccttttgt
     1321 caaaggtgca agcatcgatt ttgtagcgat cccttttacc tttgcaacgt aaactttatc
     1381 cacttcatgt tttggatgtg tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa
     1441 gatccccgaa gtatcgtaat ccagtcgacc tacaggatag atacgttctt ttaccatcgg
     1501 caaaagatcc gtaacaactt ttcttccttt gtcatctgca acagcagaaa taactccttt
     1561 aggtttgtac aataaatagt aaccgtattc ttcttgataa attgggactt catccacttc
     1621 cacagtgtca tgtttcccaa cttttatccc taattccctt acaacttgtc cgtttacttt
     1681 tacacgtcct gcaaggatga attcttctgc ttttcgacga gaagtgatgc cagcgtgggc
     1741 aattactttc tgcaatctct ccattacaat tattctcctt tattcattcc atcaaagaat
     1801 agatctggtg acacgtcttc ttcaagtgaa atttccattt cttgtgtatc aggcagttct
     1861 tctaatgatt tcaaaccaaa ataatctaag aagtagccag ttgttccata taatatcggc
     1921 cgacctggtc cttctactct acctttttct tcaatcagtt tatgggcagc tagacgctgt
     1981 accgctcctg aaacctgaac tcctcgaatc tgctcgattt cagctcttgt gattggctgt
     2041 ttataagcga tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct
     2101 tgcgcatatc gcttaagtaa gggagccagc tcttttttag tcgttaatac ataatggctt
     2161 tccgtttcaa aaatatgcaa agctgagtgc gagttttgag tgtaagattc ttttaattct
     2221 ttcactaaac ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa ttcttcatta
     2281 ctgatccctt cctcgccaac aataaaaaga agggcttcaa gttcacttaa tttcgtcatg
     2341 ttcggtatct cctactgctg gataaagcat aattgattca tagttttctt tttgttgcgc
     2401 aatgatctgc ccttttttca tcaattctag caaagccata aaagtagtga caacttcact
     2461 tttagaaggt gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc tttttatacg
     2521 ctcttcgata aataccattc gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc
     2581 cactttttga gaaaatttct tacgttctaa gattttctgc atggcacgga tcaaatcgat
     2641 ttttttgact tgtcctcgct tcaacggctt ttcagcatcc gctaacccat cgatgttcat
     2701 aggttctttt gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc tttcagctgc
     2761 atatttatat ttacggtatt ctaaaagctg agtgaccaat tcttctcgag gatcttcttc
     2821 ccataattct tcatcgtctg tgccgaattc ttggacagga agcaatgtct tgcttttgat
     2881 tgccatcaat gtagcagcca tcactaagta ttctcctgca acagctagct cgagtgtttt
     2941 cattgtatgg atataattca tatattgctc cgtgatcgta gcgatcggta tatcatatat
     3001 atcgatttcc atttgctgaa tcagatgaag cagcagatcg agaggaccct cgaatacatc
     3061 taatttaata ttgatctccg tcaactcatt cactctttct tctttttctt tgctagccat
     3121 tttccaatgt aaggctctgt gatttcagtt gcacggatgg agacatccgg gtacttttcc
     3181 tgtaattcat ctaacatttg aaagcccata ttctcaccgc gataagacgg attcaaagaa
     3241 atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa taccaataat cccttgaatg
     3301 ttggttgaac cttctggcgt aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta
     3361 tcaatctcct tcaacaaaga agttttacct gtcagtttct tatggaaact caatagtccc
     3421 atagcaattt tacggttttc tttccggtaa tgtgttaaca tcttctcacc ttcctatgac
     3481 taagcccttg gaaaatgctg cttataaaca tccgtcattc ttttcttcgt gatatgtgta
     3541 taaatctgag tcgtagagat atccgcgtga cctaacaatt cctgtactgt ccgcaaatca
     3601 gctccatttt ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct
     3661 ttgtaaatcc cagcctctcg tactaactgc ttcagatttt tccagattcc ttgccgagaa
     3721 agacctttcc catgattatt gacaaataca tgcatttcag atgcgtcctt tacaagtaat
     3781 ggacgtgctt cgtctaagta gcgttccagc cattggatcg cataatcacc taaaggaatg
     3841 atccgctctt tatctccttt tccaactgtc tgtaccagtc caagagaaag atgaagatct
     3901 cccaatttca atccaatcaa ttcactgacg cgcatcccag tagcatacat gacttccaag
     3961 attgcacgat ctcttatccc taacgttttt gtcgtatcag gtgcttcaat cagcttttct
     4021 acttctgtca gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt atcgatatgt
     4081 tgcatcggat catgatcagt gatacgctct tgccgtaaaa actgatggaa tcttctgaga
     4141 cttgagatca tccgagtaac agttgctgtc gctttttttt cttcatgcaa cttctccaga
     4201 aaacggatga ctaaaaagcg atcgacatcc tgccatgaag caattttctg ctcctgtaga
     4261 aaatgcaagt actgttccaa atcacgttca tagctttgtc gtgtattggc ggacaatccc
     4321 cgttcaatat tcaagtagtg gagataatcg ttgatttgct ctttcataat aatccccctc
     4381 atgcctctct atcatatcag ataccgcttc attctgatag tctttttcca taatctgagc
     4441 aaagaaaaag ccgtctagac actcagacgg actggtttct tttttcggct ctataattta
     4501 tgggactgat gaatcagaat tgattcttca gccccatttt cattttaggt attaaa
//
LOCUS       DACDID010000168         4435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDID010000168 DACDID010000000
VERSION     DACDID010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.173"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(63..959)
                     /locus_tag="IV236_002415"
     CDS             complement(63..959)
                     /locus_tag="IV236_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8772578.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(952..1632)
                     /locus_tag="IV236_002416"
     CDS             complement(952..1632)
                     /locus_tag="IV236_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772579.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1633..2415)
                     /locus_tag="IV236_002417"
     CDS             complement(1633..2415)
                     /locus_tag="IV236_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8772580.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2408..3325)
                     /locus_tag="IV236_002418"
     CDS             complement(2408..3325)
                     /locus_tag="IV236_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8772581.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3489..3812)
                     /locus_tag="IV236_002419"
     CDS             complement(3489..3812)
                     /locus_tag="IV236_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8772582.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1305 a          955 c          768 g         1407 t
ORIGIN      
        1 ttctctatta aatatatata agataataca tttttctact agttctcact aatcttgtat
       61 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
      121 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
      181 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
      241 gtttgcgttc ctggtttgat aggattggcc actgttaatc gaatgaatgg atcctctttt
      301 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
      361 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
      421 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
      481 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
      541 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
      601 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
      661 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
      721 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccaa ccatgtattc
      781 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
      841 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
      901 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca tttaatcaaa
      961 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagcttccc
     1021 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
     1081 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
     1141 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
     1201 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
     1261 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aaaagttctt cgatatcaaa
     1321 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
     1381 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
     1441 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
     1501 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
     1561 aagaacctcc tccagtaagg aatgaacagc ttcatcgtct tcgataatca aaatagtttc
     1621 cagcaggacc acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
     1681 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
     1741 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
     1801 gcctttgatt tttgcgaaaa caccgggaat gaaggccgtt cccatgatat aaagaaaaga
     1861 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
     1921 tgtccctaat gtcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
     1981 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaagca tggctagtcc
     2041 cgtgatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
     2101 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
     2161 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
     2221 ttctaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
     2281 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
     2341 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
     2401 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
     2461 gcccaactag gtgaatacca tccacaggaa cttttgcttc taccaatgct tgattgatgg
     2521 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgt acaaaataat
     2581 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
     2641 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
     2701 cttcatgcaa aataccatag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
     2761 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
     2821 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
     2881 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
     2941 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
     3001 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
     3061 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
     3121 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
     3181 tagacttacc cgctccattt tccccaatca atccatagat cattccacgt ttgatttcga
     3241 ttgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
     3301 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgatga gtgaagtata
     3361 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
     3421 aatgtcaaaa aaaacaatcg gacaatacgt gtctttccta ttatcaggca gtttgttata
     3481 cgcttgtatc agtttcctga ttctttcttt tcgaccaaag tcgtttctct gtattcctct
     3541 tcactgaaaa tttttgcttc tttcagatca agcttgagta attttaccag accggttata
     3601 aggataacta tagcaagact ccctcccaga aaaattagac tgtctcgcag acgtggttgc
     3661 gttgtcttcc aatcaaagag cgcaccaaac agtgctaatc caagtggact ggccaccatg
     3721 gtaatcgttg ttatcgttga cataagtcgt ccttgtatat gcgttggaac ttctcgctga
     3781 agccagacca tataaaaagc aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa
     3841 taatacatgc cgtttgggat ttacaattag ctaccaaata agaggttatg acaaaagact
     3901 tttcacaatc ccttcttccg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     3961 acaatattta tcaaacatga gaagcaattt ttgtaaattc cttcttattt cttgaagctg
     4021 accacttctg tcacaacctc agaagcggat aaataatatt gtttgatgat actttatctt
     4081 tttcccgttt gtttatagat cgaatgatat tatagaccaa gaatcctacc tattaacata
     4141 gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag tgccagaaaa gttaggatta
     4201 aggtaactgc gctcagcatt gcttcgccca gtacgattct cgtaactcgt gaactcgaac
     4261 gataatcgca attaacgttt tgagaactgt gaagctttac gggctttctt aagacctggt
     4321 tttttcttcg tatcattgaa taagtttgaa aaaccttgat ttataaaggt tttcccgtaa
     4381 atatatttta ttatatttga ttaaatttga aatgtttgtg ttctaaaatg tgttc
//
LOCUS       DACDID010000169         4313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDID010000169 DACDID010000000
VERSION     DACDID010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.174"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IV236_002420"
     CDS             complement(<1..164)
                     /locus_tag="IV236_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772583.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFT"
     gene            complement(584..1705)
                     /locus_tag="IV236_002421"
     CDS             complement(584..1705)
                     /locus_tag="IV236_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8772584.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2033..3442
                     /locus_tag="IV236_002422"
     CDS             2033..3442
                     /locus_tag="IV236_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8772585.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3439..4167
                     /locus_tag="IV236_002423"
     CDS             3439..4167
                     /locus_tag="IV236_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8772586.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1420 a          779 c          812 g         1302 t
ORIGIN      
        1 ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt atatcctcgt
       61 ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg cataccagtt
      121 tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt tccttgatgg
      181 ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt atacaacaac
      241 agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta tgctcactga
      301 tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat gtccggaaag
      361 cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg aaaaatctat
      421 ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat ttcttgctaa
      481 aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa aattttagta
      541 tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca ggtctgtaac
      601 gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt ttcaattgaa
      661 acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc gttgcgatta
      721 aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt cgtttaatgg
      781 tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct tgatacatgt
      841 cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg attttttgtc
      901 ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca ccgccgacaa
      961 gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct gctacttcaa
     1021 tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag aaatagccgt
     1081 cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc acatacgttg
     1141 cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc gcatttgtca
     1201 tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc aaatgtagct
     1261 tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg ttcaacggaa
     1321 tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc tgagcagaaa
     1381 ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt tctggcgctc
     1441 tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag aggcgctgat
     1501 ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc gcatgaagta
     1561 acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag tcgctaatta
     1621 agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat ttttgaaatg
     1681 aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg gataagtcat
     1741 ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg cttttgatgg
     1801 gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga aagatatttt
     1861 accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga atttaactac
     1921 acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac tggtttcctt
     1981 ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa ctttggatga
     2041 tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc agataagaaa
     2101 agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc aactattcat
     2161 tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag aaatcatcgg
     2221 tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg ccaaagctat
     2281 ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt gtgtatacat
     2341 ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg atcgtcttga
     2401 tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc atgaagatca
     2461 catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga cgtccgcaag
     2521 tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac tatttaatca
     2581 atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg tgaaaaaaac
     2641 aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca ccattcatta
     2701 tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac atgtcgaaaa
     2761 taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg actcttttga
     2821 gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga cgcattattc
     2881 aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac attctcttcc
     2941 acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt atcacgaaat
     3001 gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc aattgactgt
     3061 tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat taatgaacga
     3121 atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca tgaaacaatg
     3181 gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc ctgcacgcct
     3241 tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa aacaattact
     3301 aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg aattagtcgt
     3361 tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg cattaacaaa
     3421 aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa aaagcttcga
     3481 ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga acaatttatt
     3541 accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga acggatggaa
     3601 aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca cgaagatatt
     3661 tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct aaaaaggcaa
     3721 gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc gatcgcatta
     3781 gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa caatctcgta
     3841 gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa gcgattcgaa
     3901 acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa agaaggagct
     3961 gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat tattacctct
     4021 aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac agctgctctc
     4081 gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag tttcagattg
     4141 aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa actctgcact
     4201 ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa cgacgtaatt
     4261 ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta ata
//
LOCUS       DACDID010000170         4300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDID010000170 DACDID010000000
VERSION     DACDID010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.175"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(4..1029)
                     /locus_tag="IV236_002424"
     CDS             complement(4..1029)
                     /locus_tag="IV236_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772587.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="IV236_002425"
     CDS             complement(1274..2545)
                     /locus_tag="IV236_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8772588.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="IV236_002426"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="IV236_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="IV236_002427"
     CDS             3788..4261
                     /locus_tag="IV236_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8772589.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
BASE COUNT         1371 a          813 c          665 g         1451 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact
//
LOCUS       DACDID010000171         4288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDID010000171 DACDID010000000
VERSION     DACDID010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.176"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..965)
                     /locus_tag="IV236_002428"
     CDS             complement(<1..965)
                     /locus_tag="IV236_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8772590.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKE"
     gene            complement(1073..3100)
                     /locus_tag="IV236_002429"
     CDS             complement(1073..3100)
                     /locus_tag="IV236_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8772591.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3245..3976)
                     /locus_tag="IV236_002430"
     CDS             complement(3245..3976)
                     /locus_tag="IV236_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8772592.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1192 a          892 c          704 g         1500 t
ORIGIN      
        1 aattccttgg ctacttttcc gtctattgta aaatcttttt caaataccca ttgattggta
       61 tagttgatcg tagtatttcc tttttttctc gaaaattttc cattttcatt ttcatttcct
      121 aaaacagtca ttgtaaggtg atattcacct ggggttagcg gttgaccatt taaagcagtt
      181 ggaaaagaaa aatttgtatt cggcgcaatc tgcaatcctt ctttttcctc ttgatataaa
      241 acttcagaat gtcctttttt tgtaattttt gtttcaataa ctacttgatt gatataggtt
      301 ttttgatcat tttgaacgtt tgctaagatg acatttcttg cattgatttg atctggttta
      361 acttcatgaa gagtaaggtt cggagcaact tcgttcagtg tctgttgcat caacaaagca
      421 ataatatagg tgtattcatt ctcaatcgat aagctttgat ccttagtttc tgtttggtcc
      481 ttttccgtct ggatttcttg gaatgtgatc ccaccagcca atacaccatc aaatcgctca
      541 ttcggcatcc tgacatgaaa agcaacggtt tggacacttt taggttttag cgtcactatc
      601 tgtggatagc tcacataatc taccagatca aagatcaggc tcatgtcttt ttcaatctcg
      661 ttttttccgt attcaaccat cacatttgaa ttggtggttg cgctattcac agaaatatcg
      721 attttcactt ctttttctgt atcatttctc aattccgctt ttacttctac ttcctgatcc
      781 ggatcaagtt gaagatcgaa gtaagttttt ctcttgtcga tttgattctc tgaagttatc
      841 ggcgtcactg aaaaattaaa tgcactagct gacactgata tattgttaaa aagtgcccct
      901 aaaataacta ggagtgttat aaataattgt tgttttggtc tcaagaaaat cgctccttta
      961 atcatatgta agagaagact ataacaaaat cttgtctctg tgggacacaa gagcctatgg
     1021 tacctgtgcc cttcttgaaa ctcaataatt ttgttatatt cttctcttcc tgttaatttc
     1081 ctgggacatc tgcaagtatc caagatagtt tcccctcaaa ggttccgatt ctttggtttt
     1141 ctacaggaat atctattctg attccacgat gagctgtaga atctaaaaga gaggacaaat
     1201 cgctttcacc cttttgatca ctttcgaaaa tcgtaacagc ggatgtccct ataaccgttg
     1261 tattttcaga ggtaacaaag gaaagagctt ctggaagctc aaaatctcca tttgtaagaa
     1321 ctttctcttg tttcaattgt aatttccaac ttccaaaagc ccgttcatct ataattttca
     1381 acccacctga aaactgtccg aattcactta atggtctact ggatatttta tgttttccaa
     1441 aattgacata ttgaggagca gttgagagat aaagtttgcc ttttctgtaa taaaaataga
     1501 tatcctctgt attttcggag aagatgcctt ctgtattcgc tgaatccgag tcaaaaacat
     1561 acccttcaat atcaatagga gtttctttat acttctctcc tattttccca gtgattgttt
     1621 tcatttcagc aatatcaatc tgactgtctt tatctttata ataaacatgg atatctatct
     1681 ccttcagctt tgcgatcttt aggtttattt cattcgattg ctcaatcaaa ttcccgtttt
     1741 caaggtattc aaaatgcata gtgagtactt tttcaggaat tacttcctgc aaagttgtct
     1801 ccaattcgat tattaacgta tctccagcaa taaaataatc tgtcagattt agcgtgactc
     1861 gatttccggt tatcaagata tctttggcat caatttgatt tccattgatc aagatatgct
     1921 cgggaatagt taattcttgt gggaggtcga tactagtctg cattttctta tattcaagaa
     1981 aatccccctc gaccttcaat gtatttctga tagttatctt atcgtttcct ccagcttcag
     2041 cgatggcccc ataatcacta tcgttgactt ttacatcagc tgttgatgtg acattcgatg
     2101 gaagtcgcgt gacagcgaca gcattttctt gaaaaaaagt acctgttgaa gaagtaaacc
     2161 cccaatttac ttctgtagaa ccaaactgcc taatgagatc aggtataatc cgagaagagt
     2221 gaccgacacc ttccaaatca taagtaagac ttctttgctc cgcgtcccat ttaattatta
     2281 acaagcgcca tgtgccatta gaaagaaatg taggagctac cgtcacgcca gtatgctctc
     2341 cagaataatt attattattt ggtttaggcg tgacaaaagc gacatgtcca tgccctctat
     2401 tatcagcagt tatctctcga tccattcgat cagaagaacc actattttta tacgtatcaa
     2461 actcaatcga gagcgcattc gcaatgtaag gttggccaga acgagtagaa tatgagccaa
     2521 ttcccatacc tggcgatcca ataacttgac tgggattact agccattcta ggatcgccag
     2581 tcaatgtaaa ggtcatgcca tctcccgctc tatctcgttg atttcctaag tataaataag
     2641 atttaaatgt aaaatccgag agtaaactaa tcttcttatt tgaccaaata gctcctcttt
     2701 gagacctttc tgctgggttc aactgcagta acttcccatt ttccaatatg gacgtgctag
     2761 taccgatagg tgtttgaaaa attttatcga tagaaataat attgggatcg tcaggcagct
     2821 caaccgcaga aattggtgca tcttctagtc cttgtttgtc cgaaccctca gcttctacgt
     2881 tgtcagtggt ctctccacta ctcgaaatac ttaaatcctc actgctttct tgtgtctggt
     2941 tggattctat taaactgtcg aatgtcgaag tgagtttgct ttcttcgtct cttctatttc
     3001 catgctccaa ctgattcaat tccaaagagt accctatgat cggaaagata ttagtcacta
     3061 agagactgct taaaatgagc acgatcctcc tctttttcaa tatatctttt cctcctgatc
     3121 gttaaattcc tagatgaact attcgtatcc aaacaaattt agcctagcct ttgacaattt
     3181 ttgctcgatt acctcgcaag acaatatgac tacgtattta tttttaacag ttcttttcag
     3241 taaactagtt gttaggtgta tcttctaatg tccaaactaa tttagtggaa tattgttgcg
     3301 ctaacttgac agatttaccg ggtaccgaaa gctgtaccgc atcttgattt tcattttcat
     3361 ttgcaccaaa tcgatagacc caagtcccca cacctgttcc tgatttagca ttaattgcta
     3421 acgtattgtt tcctcccggt gtcaacgtta attcagaatt caccgtttgc ggcgtatatg
     3481 ttggatcaac caaagaaaca gcctgccctt tcgtaaattt cagctgtgct ccaactagtt
     3541 catccccact ggcagtttta aactgttctg gttgagagac acttaatttc cacccagata
     3601 aggtacttct cctatctgtc acttggacat atgtcggttt tctagaccca tcgttcatca
     3661 catcagcagc tgcataatac gttttatccg cagtagaaat tttttgagtc ccaaatttga
     3721 attttgatcc ataatcaatc gataatgccc ctcctgttcc agggcttgga ggatctgatg
     3781 gatcgacagg tttattggga tcaggattct ctggatcaac aggaggagtt acaccctcat
     3841 ctcctgcttc aaatgtaatc gttccttctg tgttatattc ctttgggtat acttcagttg
     3901 cagaaacaaa agtcgtactt gctaccatgc tgaataacat cattgctgct gttaatacga
     3961 tttctttttt catcattgac attcctcttt ctttatttac taaacatatc agtcgattgg
     4021 attaatccat agcaaaattc tgttttacga tacttatgaa ataaaagaac atagacctat
     4081 atttggtcct atgtatcgat tagttataaa tgattatcag cccgatttga tttctagttt
     4141 ttattgcaac ttgatgcaaa cacaatctag aaatacagct gtcatagcct ttaaaataat
     4201 tcgtaagtcc ccctcctatc tatcaaaatg tgacttgact gcctagactt caatcaatat
     4261 taattataat taaataaaaa atgtatat
//
LOCUS       DACDID010000172         4193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDID010000172 DACDID010000000
VERSION     DACDID010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.177"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(599..1141)
                     /locus_tag="IV236_002431"
     CDS             complement(599..1141)
                     /locus_tag="IV236_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772593.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(1918..2108)
                     /locus_tag="IV236_002432"
                     /pseudo
     CDS             complement(1918..2108)
                     /locus_tag="IV236_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2228..2590)
                     /locus_tag="IV236_002433"
     CDS             complement(2228..2590)
                     /locus_tag="IV236_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772594.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2594..2902)
                     /locus_tag="IV236_002434"
     CDS             complement(2594..2902)
                     /locus_tag="IV236_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772595.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(2911..3330)
                     /locus_tag="IV236_002435"
     CDS             complement(2911..3330)
                     /locus_tag="IV236_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772596.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3323..3556)
                     /locus_tag="IV236_002436"
     CDS             complement(3323..3556)
                     /locus_tag="IV236_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772597.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3553..>4193)
                     /locus_tag="IV236_002437"
     CDS             complement(3553..>4193)
                     /locus_tag="IV236_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8772598.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1177 a          935 c          605 g         1476 t
ORIGIN      
        1 aatattttct ttcagttttc cgactatggt aattcggtca attgaccact gcatttttaa
       61 gggaagggtc aaactttgat actttaaccg cctgttagag ggggctgtta atttttccca
      121 ctcatttatg atcgtgcctt ttttactcaa attttgttcc tcctcaaaga aaaagagact
      181 tctggaaaga attttcaaaa gttcacgtaa ctcttaaaaa ttcattacca aaaaatctct
      241 agtcttgaag aaatctaaaa atattcgtat aatgattaag aagttacgga gctagtgcga
      301 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
      361 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
      421 caaaaaataa aaccgcaccc tttgaaaagg gcgcccccaa acttcgctaa cgctaggttg
      481 gccaaaaaat ggacttgcca ttttttagca aatgtcagct tcagggatta gtggtttagc
      541 cgtcaactcc ttatcctcgg cacaaaccgc ctccggtacc gttgactgca catcgtaact
      601 accactcgat ctcatcttca gaacgctcta atttacgctg tgagacgttt tgacgataaa
      661 ataaggtttt ctcctgctct aacacttcta attcctcaga aagccccata tccttttctg
      721 caagaccaaa acggatagac aaacgagtga tcgatttttg aaatgcgttt tttaatcgct
      781 ctaaacgctg gcgtaaagag cgattttctt ctttcagctt tttattttcc acttgtaaat
      841 ctgctaattg ttttctacca gacaaatgac gctcttcttc caatgtagct acatactcat
      901 ttaattctgt attttgctca agcatctgta aatattgctc actcatgtta tcagcatttc
      961 tcttggcatt tgttgctctc gtagctcgtt gatacaaatc aacaaaatca ttcccagata
     1021 aatgatacga accatcagga agcctcttga aactttctaa aggaacaata tctttttgga
     1081 aaatgaccgc catctctttt tttacattca gcatattttt cttctcttct tcaatcttca
     1141 ctctctcaga agccaccaga gccttttctc gctcattttc ttctctttgc ctagaaaaat
     1201 atacctcaac tcatcagaaa cgctctcacg ctcttttacg gccgtttgac gcaattctga
     1261 aatctcgctc atgatttctt tatattcgtg aacatctttg attttgttag taccgactaa
     1321 ctcacgctcc caaccaaaat ctgccatcaa acgttcaatt gactcaactt cctgattcct
     1381 aaaattattc caaatcactc gaccattctc tttatctgct cgtaaaccct gctgttgcaa
     1441 ggctttgtta aaacttggtt gcttttgaac accccgttta tacccttcag caacaggaac
     1501 aatattcaaa tgcaggtgcg gacttacttc gtcattatga ataacagcat tgtagacgga
     1561 taaattagga ttccgttctt taaattcttt gaaatattca actaaaattt tgttggcaat
     1621 cactcgattt tgttctgctt gttgttccga tatgccagta gaagaaccat catcacgagt
     1681 agtacgaaaa tcatcaagag taccaatttg cacgataaat tctcgctgtg gttccaattt
     1741 tttatccttg cgaacctttg ctaaatagtc ctcgattttt ctatcggctc ttttctgctt
     1801 tgcattatat ttttctacgg cttcaccgaa taattcttca tatttttcac gaatatttgt
     1861 ttgttctaaa tagatatttt catccgattt tgtgaaatca atatgcttat gccagtcatt
     1921 gattttttgg ctatcagtta actcacgatt attatgagaa atgcttgtat tctttgttcc
     1981 ctttttgaac gaaatactga accccaagtt aaaaccctcc ttcggctgtc gctaataaaa
     2041 agagtgtaaa cctttctgtt actctttgtc aaagagtaac attttcctaa ttgtgacatg
     2101 gctgtcacaa ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa
     2161 ggagccgaaa gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcacgcaaaa
     2221 taatcgctta tcgtaaattc acgtcattaa atattgacgt aaatttactc tcactcatct
     2281 gacttttctt attcggtatc actccgaata agccgtcaga ttcatcggga taatctcgtt
     2341 cgcttggcgc tgatttttca tcagcaagcc actcattatc cactgatttt tcgtgttgct
     2401 gatcgtcaga aatcgacgat agccaccgct caaaatcagc aaaagtttca aacccaaaac
     2461 gcttccgaag agccttgccc attgcagcat tttctttctc aatcaagcgt tttttctgtt
     2521 ggcgaagttt cgcttgttct ttttttaatt tttcttcttg cgctttaata tctgctaaag
     2581 tcattcccat aaactacatc tccttttctt tggaattaat aacggaaatt tggtttaact
     2641 gcataacatg actcattaat ttcaaccaaa attctttttc tgattcatca gttgtttgcg
     2701 ctaaatgctt atgaacaaat tcattagtca aaactgaaga ttttgctaaa tcagcattga
     2761 taaccgagtt gagaacatcc ataattcgtt caagcatttc actataatta tcttcatccg
     2821 gcaaattcaa ataatcagct aaccgttcat acatcaattc aattctatcg actgtttctt
     2881 ctctcaaatc aattttatcc atgtcacacc tcacttccag aattgccacc attttcgatt
     2941 ttttaattct tgattttcta gtaacaactg atcgtttttt tgttgctgaa gcagggtcaa
     3001 tttttgttga tcacgaatca tttctgttaa ttcagaaata tgtttgtcct tctcagctaa
     3061 ttgatcacgc aaaaactcat tctctttttc taaaggaaaa tcactgcctg tgttattgac
     3121 cggtaattga ccagtcaatt gatcactttt tgaccgataa attgaccgaa caacaccaat
     3181 caactcttta tctagcatga taatgccatt ttctttatac tgagcaaggt caaaccctga
     3241 attgaccagt tgattgcgca ctgtattttt cgatacaccc aattcgtcag caagattttt
     3301 tatggtcatc ttttctgaca aatcagtcat tagcttctcc actactttca gctaccgcat
     3361 aaatatattt aacggcgtgc ttagggataa taatcggaga gtcaatttta ttatttttca
     3421 aaatcaattc atattgcgtt acatcaagta acttaccgga catttttttg ctatccaaaa
     3481 aaacaaccac aacgtaatca cctgttaaat ttaccaacca atttttctta ccaaaatact
     3541 tttgtttttc tgtcattttt cttcctcctc tgttacgtct agccctagaa gccatgtgtc
     3601 taattctttt tttaaatcgt ctgccgattc cgcaaaggtt tctcgcaaat gattcgtcaa
     3661 ttcatcattt tgactttctt gcttcaacaa atccctgtat tttttctttg tattcttagc
     3721 tagatttccc cactcagaat gatcataaag caaacctttc accataatcc gttcagtaac
     3781 tttagtattt ggcggaaaat aatgtggact agcaaagcta tccaaacttt catgaaccat
     3841 ctcatgccaa tcagttgctt ttccccttcg taactgcaat tctagtcgcc accatgtcgt
     3901 tatttccggc ggaacaattt tacgttttct ttcttgttcc agttttttat tatacatccg
     3961 aatttgtcgt tcactggcac gactacccca atatgccgtt tctaacttac cagaacgccc
     4021 ataaattggt ttaaaagaca caggatcaac tacccgatat tgtgtaataa actcatcagg
     4081 tacatcgata atgtcgcaag caatatccgc acgtgaaaag tgaggttcga taaacaaatc
     4141 atgaataaaa tttttcatgg aactagctaa gaattgacta atcttattcg gat
//
LOCUS       DACDID010000173         4118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDID010000173 DACDID010000000
VERSION     DACDID010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.178"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            17..124
                     /locus_tag="IV236_002438"
     CDS             17..124
                     /locus_tag="IV236_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772599.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            129..2258
                     /locus_tag="IV236_002439"
     CDS             129..2258
                     /locus_tag="IV236_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8772600.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2255..3262
                     /locus_tag="IV236_002440"
     CDS             2255..3262
                     /locus_tag="IV236_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8772601.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3279..>4118
                     /locus_tag="IV236_002441"
     CDS             3279..>4118
                     /locus_tag="IV236_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8772602.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSK"
BASE COUNT         1351 a          667 c          942 g         1158 t
ORIGIN      
        1 aaacggtcaa tgcttgatgc aagacttgta tgggcgagta ggtgtggttc aagttcatcc
       61 agtctttgaa gaactcttcc atgcttttga cactcgtccg ccggttcaag agagaatgga
      121 gtgaggggat gaaaaagaaa ctttttcgga ttgtgggaat cgtttttatt agcatcgtca
      181 ctctgacagt tctattgagt ttagtcggta cggtagctga ggcgacaggt ctggttgatg
      241 atacagttga agccggaaac ctttattcca aatattctct gaacaattat caactggatt
      301 tctttgtgga tagttcttgg gattggttac cttggaattg gggggatggc ttagggaaaa
      361 gcgtgatgta tggtctttat gccattacca attttatttg gacagtaagt ctgtatttat
      421 caaacgccac gggctatgtg gttcaagaag cttacaagtt ggatttcatt tcggatacgg
      481 ctaaaagtat tggaaagaat atccaaactt tagccggtat cactgaaaat ggattacaga
      541 cttcgggatt ctactttggg ttcctattat tgatgatttt agctctagga gtttatgtgg
      601 cgtataccgg attattaaaa cgagaaacta ccaaagcagt ccgaaccgtc attaattttg
      661 tgatgatttt tctactctcg ggttctttca ttgcgtatgc gcccacctat attacaaaaa
      721 tcaatgactt tagctcagac gttagtgaag cggctcttag ccttggaacc aagattgttg
      781 tacctaattc agaaagccag ggaaaagata gtgtggattt gattcgagac agtttatttt
      841 cgattcaagt tcaacaaccc tggttactat tacaattcga tgattccaat atagaagaaa
      901 ttggcgaggg tcgggttaat aagattttat ccgtgagtcc ggatgaaaat aagggtaagg
      961 atcgggaaga agcagtcaaa gcagagattg aagataatga caatgcgaat ctcagtatta
     1021 ccaagaccat gaaccgttta ggaaccgtgg tctttttagt gttgtttaat attgggattt
     1081 ccttttttgt gtttcttctg acagggatta tgttgttctc acaaatcctt ttcattatct
     1141 ttgcgatgtt tctgcccatt agtttcttat tgtcgatgtt gccgacgtat gagagtttgg
     1201 gcaaaagggc gattattcgt ttgttcaata cgattatgat gcgagcggga gttaccttag
     1261 tcattacgac tgcattcagt atctccacga tgtttttcaa tatttcggcc acctatccct
     1321 tctttatggt agcttttcta caaattgtga cctttgccgg tatctatttc aaattaggtg
     1381 acattatgag catgtttaac cttcaaagta atgatagtca gtcaatggga aggcgagtca
     1441 tgcggaaacc gcaaatgttg atgaatcgaa aattgcgaca gcttaatcga aatgtgggtc
     1501 gaacttttgc ttttggtggc ggtgcagcag ttggtgggaa actagctacc agttctacaa
     1561 aagggaaagg tagttcaaaa atcaatagag tgtccaatag aaagaaccat tccgcgagaa
     1621 gcattaacga gctaaattct gatctgaaaa aacaagaaca accagtgggc acgaagaaag
     1681 aatcacgaat aaatttagtt ggtagaaaag ttggaaaagt actcgataca aaagaaatcg
     1741 tgaaggacaa agcgaaacaa gcaaaaaatc aagtaaccga tgcaccaact aacttgaaat
     1801 acagccttca taaaggcatg gaaaagacga aaaaagtacc agaggacttt aagcgagggc
     1861 ctttcgaaga aaaagcaaat cgaggggagt tgcgagaaaa acaacgacaa cgaagagacg
     1921 caaaaatgga tgaaaaacgc aaagtattga acgaagcatg gaatcgtcat gagaaaggaa
     1981 gggaaaatgt tccgataaaa aaggagataa aaccacagaa aaatgaacta ccagaaaata
     2041 ggtctttaaa accaagggta caagtacatt cgaatcctga aatcaaacgg aaactttcta
     2101 gtcaagaagt cattccaaac ggaagcactc agccactaag caagaacaat tcctttcaac
     2161 aagtgaaaca gagattcact tttccaaagc gaaccaatca acaagtacaa agaaaaaaga
     2221 acactcgccc aaaaccaaag ccaggtgaga aaagatgaac ttaaaaaaat tgagtattat
     2281 ttcgttaacc ttcctgacca tctcttttgt gggcatcttg atgtgtgtgg gtttgttgtt
     2341 tggtgaggac ggtgagggaa gtggtagttc tggaacttct cctggaggta gtagtgtctc
     2401 agaagtagtt ttgaaacacc gttcaatggt ggaaaagtat gccaaagaat cagatataac
     2461 agagtatgtt tcgattcttt tggcgattat tcaagtcgaa tctggaggta caatggaaga
     2521 tgtcatgcaa agttctgaat cagcaggttt atcaccgaat tcattaagta ccgaggcatc
     2581 catcaaacaa gtgtgtctct atttttcgtc tttggttaag tgttccaaag aactaggatg
     2641 cgatcaagat agtatcattc aggcttataa ctatggtgac gggtatttgg attatgtcgc
     2701 gaaaaacgga aagaagcata gttttgcttt agccgaatct ttttcaaaag aaaaatcggg
     2761 tggtcaaaaa gtcgattatc ccaatcccat cgcgattaag gaaaatagtg ggtggcggta
     2821 taactatggc aatatgtttt attgcttatt agtgaagcag tacctaacta caacacagtt
     2881 tgatgataaa acagtccaag gaatttttga cgaggcgttc aagtatgaag gaacggctta
     2941 tgtatttggt gggtcaagtc ccggaactgg gtttgattgt agtggtttaa ctcaatggtg
     3001 ttatgccaaa gttggtttga aactccctcg ggtggctcag gatcaatatg atgctatgac
     3061 tcatattgac ttaaaagatg ccaaaccagg cgatttgatt ttctttcatt caacttatga
     3121 tgccggtact tatgtgactc atgtgggcat ttatgcagga gagaacagaa tgtttcatgc
     3181 aggagatccg gtgggttgga ccaatcttac cgatagctat tggcaacagc acattattgg
     3241 tgctggtcgg tataaacaat agaaaagagg aatggaaaat gaagataaaa attgagcgaa
     3301 gtaacaagga aaagattcca aaaaagaaga aagaacgagt agtatccgtg ggcaagcatc
     3361 gaaaaatggt gcttgccctt tggcttttat tgtcttgtag ccttgctttt ggaatctaca
     3421 aaaacttcac ggccattgat cagcatacaa ctcatgagaa agtggtcgtg aaagagaaaa
     3481 tagtgaatac ctctggagta gaaaatttta ctgtagattt tgtgaaagaa tatttttcat
     3541 ggaagaataa caaggaggta attgaaaaga gaatgactaa tcttgagcag taccttacag
     3601 aagaagggct cgctttgagc caagatatgg ttcgagcgga tattcccact agttccgagg
     3661 ttgtatcagt aaaaattcta gatgttgaaa aacactcgga agaatttgtc gtttcatttg
     3721 tggtagatca aaagattaca gaagggaaaa agacacaatc aatttcttct gcatatcgtc
     3781 tgatgatctt tgaagatgaa aaaggcaatc atattgtaac gagtttaccc actatgattg
     3841 gtaagccaga aaaggcaaaa cataaagtaa gacaagtaaa aagtgattca aaaattgatt
     3901 caaagacaac ggaagaaatc accgagtttt tagagacgtt tttcaggctt tatccagcag
     3961 cctcagaaaa agaactagag cattatgttg aaaaagacgc gatgcaacca attaatacta
     4021 atttgaaatt tgtggaatta gtgaatccta tttacaaaca aaagggtaaa gcgattcaag
     4081 tgattgttgg tgttgagtat cttgatgagt tgtccaaa
//
LOCUS       DACDID010000174         4040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDID010000174 DACDID010000000
VERSION     DACDID010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.180"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..775
                     /locus_tag="IV236_002442"
     CDS             122..775
                     /locus_tag="IV236_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8772603.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            860..1765
                     /locus_tag="IV236_002443"
     CDS             860..1765
                     /locus_tag="IV236_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8772604.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1767..2399)
                     /locus_tag="IV236_002444"
     CDS             complement(1767..2399)
                     /locus_tag="IV236_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8772605.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            2719..3243
                     /gene="infC"
                     /locus_tag="IV236_002445"
     CDS             2719..3243
                     /gene="infC"
                     /locus_tag="IV236_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8772606.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3315..3515
                     /gene="rpmI"
                     /locus_tag="IV236_002446"
     CDS             3315..3515
                     /gene="rpmI"
                     /locus_tag="IV236_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8772607.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3568..3927
                     /gene="rplT"
                     /locus_tag="IV236_002447"
     CDS             3568..3927
                     /gene="rplT"
                     /locus_tag="IV236_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8772608.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
BASE COUNT         1358 a          708 c          836 g         1138 t
ORIGIN      
        1 ctttttttct tctttgacta gaaaaaaata caattgacac tactagatat tgggggtata
       61 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
      121 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      181 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      241 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      301 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      361 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      421 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      481 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      541 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      601 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      661 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      721 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      781 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      841 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      901 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      961 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
     1021 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1081 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1141 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1201 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1261 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1321 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1381 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1441 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1501 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1561 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1621 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1681 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1741 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1801 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1861 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1921 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1981 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     2041 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2101 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2161 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2221 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2281 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2341 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2401 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2461 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2521 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2581 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2641 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2701 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2761 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2821 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2881 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2941 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     3001 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3061 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3121 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3181 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3241 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3301 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3361 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3421 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3481 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3541 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3601 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3661 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3721 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3781 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3841 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3901 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3961 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     4021 taacgtataa gtctaaaata
//
LOCUS       DACDID010000175         3992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDID010000175 DACDID010000000
VERSION     DACDID010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.181"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(101..1606)
                     /locus_tag="IV236_002448"
     CDS             complement(101..1606)
                     /locus_tag="IV236_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8772609.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1603..2289)
                     /locus_tag="IV236_002449"
     CDS             complement(1603..2289)
                     /locus_tag="IV236_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772610.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2482..3903)
                     /gene="gndA"
                     /locus_tag="IV236_002450"
     CDS             complement(2482..3903)
                     /gene="gndA"
                     /locus_tag="IV236_002450"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8772611.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1097 a          850 c          686 g         1359 t
ORIGIN      
        1 cgctagtttt tttataatga catccctgaa tttctcactt gcttgtattt tgtaccttat
       61 tctttgttgg atatataaaa aaacaagtaa agcacatgct ttacttgtct tctgcctttg
      121 cgataggaat aaagattcta aaaattgtcc cttgccctac tacactttct gctaagatac
      181 gtcctttata gttttcaacc agttgtttag cgatagacaa ccccagacca tttccaccct
      241 tcgttctagc tcttgcttta tccactcgat aaaaacgatt gaagatctta ttaaggtctt
      301 ctttgggtat tccttcgcca aagtcctgaa tcgcgatctc aaattcattc agagtggaag
      361 aaatagagat atggacttct tttcgtgtag ttgaatactt tactgcgtta tccaatatga
      421 taatgattaa ttgttcaaag tgattacgat aaatttgcag ggtcacttct tttgtcaaat
      481 catcatctaa agtgatcaca tagtctggat ataatatctt gaaattatta taaacttgat
      541 aggtgacttc ttttccttta gttgtttcat tcgcataatg aacatctact tgttctgctc
      601 ttgataagtc tagcatctct tgaactaagc ttttcatacg gctgatttct tgcatgctgg
      661 cgcttaatga ttcttctaat atctcaggat catctttccc ccaacgattt agcagactaa
      721 gatgcccttc aataattgca acaggtgtcc ttaactcatg agatacgtct tccacgaatt
      781 gctcttgttg ttcaatatac aaacgcatac ggtcaagcat ctcattgaat atttcagcta
      841 aatctgccaa ttcatcattt gtgtcgattt caggcatgtg gatatctgat tgcggatctt
      901 tacgtatagt atccattgta tctctgagta ccttgagtgg ttttaagaaa taagaagata
      961 aaaagaaacc taaaatacta cttaagatca aagaaacaac ttctaaaaca ataagagtca
     1021 ataatagctt atttcgaata tcatagaaag aagataattc atagaagctt tgcgcatagc
     1081 cgatcttttc tcttgtacct ttagaatata taggctggat agataaaaag ccagtttttc
     1141 cttctagtgt aacgatcgtt ggaactttac gtgtagtctg aatcaggttt cgactgtttt
     1201 catgggtttt aaaaatcaag ttctcgtcca aatcataaac ataaagatcc attgatggct
     1261 gccctagctc agctataaag gaatcgatct tcattaagtt tccttcgacc agtgaagtat
     1321 gatctacgac ctcatctccg tcatttgtcg ctttcgtcag attacgatac gtatttacta
     1381 atgtcagttc ttcatccgag ttagccaaac gagaagctac ttctgaaatc gtacgctcta
     1441 cattgtttct ttcttttgcc acaatcagat tgatagatga tttataggta atgacagcga
     1501 aaattgtaaa cacaacgaat atgaagaaag aactcgcaaa ggcccacttg attgtcaagg
     1561 agggcccttt cagttctctt ttttccgatt ttttcggctt tatcacgagc gcatcacata
     1621 acctgttccg cgtacagttt ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg
     1681 aagataacga atatatacgt ccactacgtt tgtttcaact tctgtttcgt atccccacac
     1741 tttatttagt aaaacgtcac gtgctaatac cacgttcacg ttttccatta aagtaagcag
     1801 caactcgtat tctctttttg ttagttcaat tacttcattt ccgcgacgta cgacacggtt
     1861 ttctttttcg atagtcaaat cgcgataagt aatagtagtt tgtttagcca catttttatc
     1921 gccttcaata tcgattcgac gcaacaatgc acgaagacgt gctaataatt cttcgatagc
     1981 aaatggtttg acgatgtagt catctgctcc gtggtcaagt ccagatacac gatcgatcac
     2041 agaatctctt gcagtcatca taatgattgg tgtatttttg acttggcgta cacgtctgca
     2101 gacttctaaa ccgttcaatt ctgggagcat cagatccaac aatatggcat cccactcatt
     2161 atttaatgcc gcatctaaac cagttcggcc gttgtagtgg acttctgcgt tatacccttc
     2221 atgcttcaac tctagctcga caaatcgtgc caagttcttt tcatcttcaa tgattagaat
     2281 attgctcatt tattatttta tcctttctat tctactggct ttttaaaaac tctatcatta
     2341 taccgataat atgcttaaaa atctatagtc attttatcat ggatagcaat tttaaaaaag
     2401 agctgaagtc tactcataac aatcgacttc agctctttcg tcctcttttt attttctcat
     2461 ttcctaacag ccgaaagact attattcttc atggtaccat gagtaatgga agattccttc
     2521 tttgtcgaca cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt
     2581 tgcaggcaga cgttctgctc tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg
     2641 tacaggtacg ccagcttgga cagccaaggc aacgacgtct ctgactgatt gctgatattt
     2701 tttcgtgatt tcaacgaagt aatcatctag caataaattt tcgatctcag gatttttgtc
     2761 ataggcatcc gtaatttttt gcaagaatcg agctcggatg atacatccag cgcgccagat
     2821 ttttgcgatt tcaccaaatg gcaagtccca atcaaattct tttgaagcga cacgtaattg
     2881 agcaaatcct tgggcataac tcatgatttt actgaaatac aacgcttctc ggattttttc
     2941 gaccaattct tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct
     3001 tgcttgtacg cgttcttctt tataagcaga aatatagcgg gcaaatacag attcagtgat
     3061 caatggaagt ggcacaccta agtcaagcgc gctttggctt gtccatttac ctgttccttt
     3121 attcccagct gcatcaagga tcacatctac tactggttta cctgttcctt catcgtcttt
     3181 acgtgtcaga atgtcagcag tgatttctat caaatagctg tctaattcgc cttgattcca
     3241 ttctttaaag atatcagcca tttcttctac tgaaaggttt aaaatattct tcattaagtc
     3301 ataagattca gcaatcagtt gcatatctcc atactcaata ccgttgtgta ccattttcac
     3361 ataatgtccg gctccatttg ggccaatata tgtgacacat ggtgctccgt cttctgcttt
     3421 tgcagaaatt tgctctaaga ttggtgcaac tagcgcatag gcttcttttt gtccaccagg
     3481 catgattgaa ggtcctttta aagctccttc ttcaccaccg gaaacgcctg taccgataaa
     3541 gttgatgcca gagttggcta actcttcatt acgacgcatc gtatctttaa agaatgtatt
     3601 tcctccgtca atcaaaatat cgcctttatc aaggtgagga agtaactctt ggattgttgc
     3661 atctgttgcc ggtcctgctt tgaccattag caagatccga cgtggtttct cgatagattc
     3721 tacaaattct tcgattgtat atgttgcttt gaaattttta tctgggtgtt cttctactac
     3781 agcggttgtt ttagcccctg tacgattgaa taaagccact gaatagccgc ggctttctat
     3841 gttcaaggct agatttttac ccataacggc cataccgaca acgccgattt gttgttttga
     3901 catatgaact cctcctacat acgggatttc tccctaatct cttacattat agcttaacac
     3961 ggctacattg caaactaaag aacaacatat tt
//
LOCUS       DACDID010000176         3944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDID010000176 DACDID010000000
VERSION     DACDID010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.182"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            838..1050
                     /locus_tag="IV236_002451"
     CDS             838..1050
                     /locus_tag="IV236_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772612.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1328..3127)
                     /locus_tag="IV236_002452"
     CDS             complement(1328..3127)
                     /locus_tag="IV236_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8772613.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3251..3847)
                     /locus_tag="IV236_002453"
     CDS             complement(3251..3847)
                     /locus_tag="IV236_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8772614.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1409 a          701 c          682 g         1152 t
ORIGIN      
        1 cagataataa cggaatatta tcatcgaaac agctaacaaa attgtcataa aaaacgagat
       61 ggttgatatt agaacttgtg tttttgttta ggtctagaga taaagagaga caatttccaa
      121 atatatgtaa tgaatacaaa aagacgtttg tcgcggatta ccgttacaaa cgtcttttat
      181 tgattttccg agatctcatc agtcgccatt tggaatggtt cactccttat ctagattcgt
      241 ttaatgctcg gactgcttgt tcgattgtaa taccaaacgc aacgttattt tcgttttgtg
      301 catcaatcgc cataaaacgg ttagaagttg tatcaaaagc tacgcggtat tgcatatttg
      361 tttttgtaaa cgtcataaaa ttcatctcct ttgtaatgta atttagttct tgaaagtttc
      421 ctttaaactt tcgaacttat accaagttta gcatcgtacg ttttttgatg caattaaaaa
      481 gcatttatca ttttccttat ttattttttc ttaaatggtt tagctatttt cttttaatcc
      541 gataagtagc ccaatcatca aggttacaaa tactattgct tgaatataaa tcatatgctc
      601 acctctttat aaataactta gcaatataaa gaaaaaaacg cacgaacaac ataggaatat
      661 aaacaaacgt ttttgtcaca acgtctaaga tcgtgttaaa aaatagttat ctaaaaagca
      721 gaaagatact caaaataaaa aataaaaaaa tggataaaaa cgttttcaaa tacaaggtaa
      781 tttgttatta taaaataaag aaaaaacaaa ataaaaataa agaaaagagg gaatgaaatg
      841 gtaaaagcag aagtaggaag atcttatata tgcaaaccga tcggagtatc tcatgaacta
      901 atcggctgta ttgaacgtac atatatcaac acggctgtcg tgtatgttga aagttatgac
      961 caacgagact atcaacttat caatgaaagc aagtatcgca tgctcgtaaa attctgtgat
     1021 attagcgcac ctgctgaatt ggtttcttaa acagtatttt tgagaattcg caagaactcc
     1081 cttataatat aaataaagac agaagggagc gagaagatat gccaaaaaga aaaagttcag
     1141 ggaaaatggg aattattctt gcagtgatct ctgcaattcc tgctatttta gctattgttc
     1201 gtgcgatgaa ggaatcaaga gaaagttaag cacacattat gagcgtcaaa caaaaaacgc
     1261 atatacctgc ggtggcaggt tatgcgtttt ttgttgtata aagacttttt tcattaacga
     1321 tttttgatta gccgactccc aaaacaaaat ttagcagttt atctgaataa gtgatttctt
     1381 catctgtttg tttcacactt tcatttagta gtcccatctc atcggttatg ataccatcca
     1441 caccataaaa catcatgcga ctcatagcat ctgaatcgtt gattgtccat acatatactt
     1501 cttttccgtc ttggtgggca gaattaatga agttacggtt gatcgtacta tattccattg
     1561 tcagaaaatc agcaggcgtc acagggggcc caacaatatt gaaaggtaag atgtagccca
     1621 cataaaagtt tggttctgct tcttttaaat ctgaaacggt ttggtacgtc aaactttgca
     1681 aaatatgatg gttctctaag ataactttgc ggtatttatg gacaaatcgt tcgacaaggt
     1741 catccgaatc ttttttagag gtcttgatct caataagtaa tttttggttt agcttatgtg
     1801 ctttttccaa ataagaatca aaagaacaaa caggggcttg cactccgttt tcagtaactg
     1861 taagatttgt caattctttc aatgtaagat cctgtggtgt tccatttacc cctgttaagt
     1921 gacgaagatt tgcatcatgc atcactacga aatgaccatc ttttgtttcc tgaacatcca
     1981 tctctacata atcaggatga tagtctctgc ttgttttttc aagtgcttct aaagtatttt
     2041 gcgccccatt ttgggtactc acacctctgt gagagacagt cactggtttt gtctgaacag
     2101 ctgaagtcag ataatccata ttgtaaagac agacgccaat accgaaaaaa actgcaaaaa
     2161 gagtcaaacc agtgtttttc agcgcagaga aacgcaagtt gggggcttgt gggataaacc
     2221 acttagggat ctcaggcaag aagccttcgt catccatgaa atcaacaatg atataaaaaa
     2281 tccctacggt agacatcacg atattgaata ataaaatccc ttgtaaaagc gtcatagcaa
     2341 acacagcgct gatcaatgca tagtctggga aaaattgttc aaccatactt tgtgccaaaa
     2401 taacaacgat atatcctaaa gaagaaagga gtaaaatcgt accgccaatc acaataaatt
     2461 ggcccgtgat ggccaaaaga cgcgatttgg tcaatgacca gctttcccga atggctgctc
     2521 gaaaaggacg atctcgtaga atcatttccg gtaaagcaaa aatcagtcga atcccaatat
     2581 agccaaggaa aatataaacg agtaaaaagg aactgacgat gatccaacga ttagcaaaaa
     2641 taaaatccat aatgaaagcg gggattttta tttttgacag tagatcagaa ttaaaactta
     2701 atccgctgat cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa gtaatgggtc
     2761 tcactttctt cagctgaagt atcgtcaagt gtaagagttg cttcaaagag attggttctt
     2821 tctttttaat aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa actaacagta
     2881 aaatcgtcat caaaactacg attaacgaga gtagaacccc aggatgttga gaaaaaattg
     2941 aaggaatatt gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata aaacgggtcg
     3001 tactgctcaa taagggtaaa caaataaata aaataaagcc atgcatcagc aagacatcac
     3061 ggaaataagc ttgtgttcct tttaaaaagt cccaagcatt acctaagcta cgtttcaaat
     3121 aattcatcct atcacctgct cttcacattg tagcgcaaat ctaatgaaaa aggaaagggt
     3181 gcagtgtgtt ttgcagaccc tttcccagat ttaacattgt caatcaatga ctaattttac
     3241 aaaataaata ttatccaaga agaccactta gatattgacc tccataaatc aaggccatac
     3301 tatataagaa aatcgacagt ggtttagcaa tcaaaatgat tagtgtatat cgtttcactg
     3361 acatgtttgt caatccagcc atcaaacata acgcatcgtc aggagcaact ggaagaaaaa
     3421 tagctagagc aaacagcttc tcaaaacggt tttgattatc cagccagcca atatatttgt
     3481 tatatgtctt atcgctaatc atggaaagta tgaatggctt tccataacga cgccctagca
     3541 gaaaaatgat aatcgaacca atgcagattc caatatagtt atagataaat ccagcataag
     3601 ggccaaatat caggacacca gctgcagtac tgatccctcc aggaatgatt ggaataacta
     3661 cttgcaggat ttggatgaag acaaaaataa tcggtcctaa aatgatggaa tcacctacta
     3721 atcctcttaa cgcattcaaa tctttgaaaa cacctaaatt aataaaataa atcgtcagcg
     3781 cgattgatag gcctaatccg ataatggata tgaaatttat cagttttcga gaggcagcaa
     3841 tattcattat tcatcaactc cttagaggtt ctttgttaac tatactttac tagaaaagca
     3901 actaactgcg ccattttttg atatgacaga aaacaaacaa tttt
//
LOCUS       DACDID010000177         3861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDID010000177 DACDID010000000
VERSION     DACDID010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.183"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            211..1107
                     /locus_tag="IV236_002454"
     CDS             211..1107
                     /locus_tag="IV236_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772615.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1157..1546
                     /locus_tag="IV236_002455"
     CDS             1157..1546
                     /locus_tag="IV236_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8772616.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1533..>3861
                     /locus_tag="IV236_002456"
     CDS             1533..>3861
                     /locus_tag="IV236_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8772617.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1293 a          619 c          812 g         1137 t
ORIGIN      
        1 aattgatgaa caccgcggag aattgattaa gaatttagtg accaatcggg ctaagattca
       61 aacaataatt aatgaaatgt cagatgaaat tgatagttat agagcagagc tgaataaatc
      121 agattggaaa gttaactata atgaggtttc aaagaggtat caaagaggta tcaaaaactt
      181 agtgaagagt tatctcaaga tgaattaaaa atgttacaaa accttgattc tttatatcaa
      241 tctttatcaa aaactgaaga gcgattggaa gaaattattc aacaagaaat aaagcttaaa
      301 gattaccaag ttgaggttga ctcagcaatg tcaagaatga ataaatcata tcaaaatctc
      361 tattctgaga gaagtagttc ctcgcgcgaa attttttctg atattacagg aatagaggct
      421 actattcaac caaataggga tttcaataat tatgttaaag agctcagagc ttgtattaat
      481 aaggaggaca cttttgacag tgagtttgga tctcttattg ataagctgac aaaaaattcc
      541 attactaatc aagatatata taatgacata cttgatataa gagttagtgg ggaaagtagt
      601 attttttcag ataaaaaatt aatcaattct ataagaaaat tgaatgatgc acaagttctt
      661 gaattacaaa cccttaagcc aaatgattta gtagttatcc gacttgaact aaatggaaaa
      721 aaagttagtt tatcgaatgc tagtgctggt caaaagacat ctgcaattct ttctatgatt
      781 ttggcgtatg gaaattcacc actcatattg gatcagcctg aagatgattt ggatagtcaa
      841 ttaattaata acttaatcgt aagaagtatc attacaaaaa aggaaaacag acaaatttta
      901 attgtaactc ataatgcaaa tatcccagtt aatggtgatt cagaatggtt gattagcatg
      961 ggagattcaa aagaaatatc ggctgatcag tgcgggtcgg tagatgaacc caatatcaag
     1021 ccaagaatat gtactgtgat ggaaggtggc gaggatgctt ttacaaatag agcgaaacgc
     1081 tatggtttta aacaaattgt agaataacac tagagggtga gacatattat tatgtccgcc
     1141 ctttttagga ggaaaaatga aaaaaataaa aagctatacc agtatctggg cagttgaaaa
     1201 agtcatttac gcaatcaatg attttcagtt gccttttcct gtgactttta accaaatggc
     1261 ttggttcgtt ttatctttac tgtttgtcat cgtatttgcc catgtgcctc cactatctat
     1321 gatagaggag gtctttttaa aatacttagg cataccggtg gctgtcactt ggtttatgtc
     1381 gcaaaagacg tttgatggca agaaaccact gggattttta cgatccttta tcagctatca
     1441 cttgcgattt aaggtgactt ttgcaggaaa gaaagtcaaa gaacagaaga aacgatggga
     1501 tgaacccagt actcttgtaa ggagtgtgaa ctatgtatcc gattaagtat attgaaaata
     1561 atctcgtctt taaccaagaa ggcgagtgtt ttgcctacta tgaattagtg ccttacaatt
     1621 actccttttt gtcacccgaa cagaaatttc aagtgcatga taactttcgc caactaattg
     1681 cgcaaaatcg tgaaggcaag atccatgcgt tacaaattgc cacagaaagt agtattcgcg
     1741 ccacacaaga acgatcaaaa aaagaaatta ccggtagact caaagaagta gcgaaacaac
     1801 gaattgatat acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     1861 gcttttttat tggctttaaa ctaattgcga cagatgaaga agtcaatcta aagagtctca
     1921 agaaatcttt cttttctggg cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     1981 gcgattttgt ttccttatcc aatgaagaaa ttcgctgcta ttctaagctt gaaaaattaa
     2041 tggaaagcaa attggctcga agatttaaag tcaggcgagt cacaccgagt gacctgacgt
     2101 atttgattga acatatttat ggcgaaaaag ggataccgtt tgaagaatac gaatttcagc
     2161 taccaaagaa aaagctaaaa tcagaaacac tggtgaaacg ttatgactta ttacgtccaa
     2221 atcgttgttt aattgaagaa aaaccaaggt gcttacggat ggaacatgag aatcatgagt
     2281 cgtacgtagc gtatttaaca attaatacaa ttgtggggga gatggaattt ccttcctcag
     2341 aacttttcta ttaccagcaa caacaattta cattcccaat cgacacaagt atgaatgtag
     2401 agattgtgac gaataaaaag gctttagcta ccgttcgtaa taagaaaaaa gaactgaaag
     2461 atttagacaa tcatgcttac caatctgaca atgaaacgaa ttccaatgtc ttagatgctt
     2521 tggattccgt ggatgaattg gaaaccacct tggatcagtc aaaagaatct atgtacaaac
     2581 tgagttatgt ggttcgagtg agtgccgaat cagtggatga actaaagcga cgctgtgatg
     2641 aagtgctcga tttttatgat gataccaatg taaaactagt ccggcccttt ggcgatatga
     2701 tggggctaca tgaagaattc ttaccctcaa gtaaacgata tatgaacgac tatattcagt
     2761 atgttacgtc tgattttttg gcggggcttg gttttggggc tactcagatg ctaggagaat
     2821 tagaagggat ttactttggc tacaatgtgg atacaggtcg taatgtttac ctgaaaccgg
     2881 cattggcttc acaaggagtc aagggttcag taaccaatgc gctggccgcc gctttcctgg
     2941 gttctcttgg tggaggtaaa tcctttagta acaatctctt agtctactat gctgttttgt
     3001 tcggtggtca agctgtgatt gtcgatccaa aaggggagcg aggagggtgg aaagaaacct
     3061 tgccagaaat tgcggatgaa atcagtatcc tcaacttgac gagtaagtca gaaaatcaag
     3121 gtctgcttga tccttatgtg attatgaaga aaaagaaaga ctcggaaagt ttagcaattg
     3181 atattctgac ttttttaaca ggaatttcaa gtcgagacgg ggagaagttt cctgtgttac
     3241 gtaaagccat tcgaacggtg actcaaagtg atcaacgggg gttattacaa gttattaacg
     3301 aattgcgaaa agacgggtca ccaatcgcgg aaaatatcgc cgatcatatc gagtccttta
     3361 ccgattatga ttttgctcat ttgcttttct cagacggctc gattacccaa tcgattagtt
     3421 tagataaaca gctaaatatc attcaagtgg cggatttggt tttaccggat gcagaaacga
     3481 cttttgaaga atataccacc atggaattgc ttagtgtttc gatgctaatc gtcatttcca
     3541 cttttgcctt ggactttatc catagtaacc gagaaatctt caaaattgtc gatttggatg
     3601 aggcttggag ttttcttcaa gtggctcaag gaaaaacgtt atcgaataag cttgttcgtg
     3661 ctggtcgggc catgaatgct ggggtctatt tcgtcactca aaatagcgac gatttattag
     3721 atgaacgttt aaaaaataat atcgggttga agtttgcttt tcgcagtacc gatattcatg
     3781 aaatcaagaa aaccttggaa ttctttggtt tggatcagga ggatcaaagc aatcaaaaac
     3841 gcctgcgaga tttggaaaac g
//
LOCUS       DACDID010000178         3855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDID010000178 DACDID010000000
VERSION     DACDID010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.184"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..1088
                     /locus_tag="IV236_002457"
     CDS             120..1088
                     /locus_tag="IV236_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8772618.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1099..1914
                     /locus_tag="IV236_002458"
     CDS             1099..1914
                     /locus_tag="IV236_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8772619.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1982..2692
                     /locus_tag="IV236_002459"
     CDS             1982..2692
                     /locus_tag="IV236_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8772620.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2728..>3855
                     /locus_tag="IV236_002460"
     CDS             2728..>3855
                     /locus_tag="IV236_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8772621.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1277 a          565 c          857 g         1156 t
ORIGIN      
        1 gatacaatgc aattatgtca catgacgtta tatgacgtta tgtgatatct ttacatttaa
       61 tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga
      121 tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa
      181 tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta
      241 aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa
      301 ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag
      361 cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga
      421 ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg
      481 gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt
      541 tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta
      601 tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg
      661 ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg
      721 cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc
      781 attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc
      841 tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata
      901 aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag
      961 atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt
     1021 cagctttagc agatgtacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg
     1081 agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat
     1141 gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa
     1201 aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat
     1261 gttttatcag caataaaaga aataggactg gacaacagtc actctcgctt tgaaaaaggc
     1321 gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat
     1381 tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta
     1441 aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct attagaagag
     1501 ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag
     1561 gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat
     1621 gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca
     1681 acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc
     1741 gtgctagaag aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta
     1801 ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct
     1861 gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt
     1921 tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa
     1981 aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa
     2041 taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat
     2101 agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga
     2161 agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg
     2221 taaaattgaa tatattatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac
     2281 tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga
     2341 tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc
     2401 cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga
     2461 gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg
     2521 ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc
     2581 tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat
     2641 tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt
     2701 ttggatttca aggagtagat tttatttatg aagcaaatat tgcggtcaaa aatgattttt
     2761 gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag
     2821 gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat
     2881 gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg
     2941 gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt
     3001 attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat
     3061 gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt
     3121 ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag
     3181 acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa
     3241 atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt
     3301 tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat
     3361 atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag
     3421 catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga
     3481 gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat
     3541 cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca
     3601 ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa
     3661 gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt
     3721 agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta
     3781 gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca
     3841 actgttataa aaaat
//
LOCUS       DACDID010000179         3783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDID010000179 DACDID010000000
VERSION     DACDID010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.185"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1029)
                     /locus_tag="IV236_002461"
     CDS             complement(<1..1029)
                     /locus_tag="IV236_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HAP8772622.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFE"
     gene            complement(1033..1443)
                     /locus_tag="IV236_002462"
     CDS             complement(1033..1443)
                     /locus_tag="IV236_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8772623.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1461..1952)
                     /locus_tag="IV236_002463"
     CDS             complement(1461..1952)
                     /locus_tag="IV236_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8772624.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1962..2753)
                     /locus_tag="IV236_002464"
     CDS             complement(1962..2753)
                     /locus_tag="IV236_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8772625.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2746..3519)
                     /locus_tag="IV236_002465"
     CDS             complement(2746..3519)
                     /locus_tag="IV236_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772626.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1178 a          788 c          510 g         1307 t
ORIGIN      
        1 ttcaaataga aaataatctt cgattttagg aacaattact ctttcttttt ttccaaattc
       61 tttctctaac ttaaaacttg aaaatggagt gatttcttta aaattctcaa ttattttttc
      121 aggcagttta acaccaagag ttttgtctgt cttttgaata atttgatgtg gaacaagtgc
      181 accgccccaa tcccagttat tttgatcgtt ctccccttct tttgttgcca cccagccagt
      241 tagaaaacgg ccagtttcat tagttccgac tgttcttgct gcataatagg cttttccgtc
      301 gaaagcttcg tctactggtg atttccaatc tgaaaataat cctgctccga ctcgatattt
      361 agtcttttta tcttcacaat actctgaaaa aagtaaatac cacgttccat caatttcaaa
      421 tatatcaggc atttcaatca tgttaaattc attattagcc cagtagtctc ctttgaaatt
      481 ccaatctttt aaatcttcgg attcaaaata gaccaatctc cccgtcggct ttttcttact
      541 tgctggtttt ctagctccta gtatcaaaac gtattttcca tgatcttgat caaaaattgc
      601 aaaaggatcg cgccaatcat tcttatcata tccttcttga ggaacaagtt ttgctgcttc
      661 gccatccttt ttccaatgaa ttaaatcttt agaacttgca atcattaaca cttctgaagt
      721 ttttccatcc aacttagctt tgcgattatg tccagtatac aaagctaaat actcattgat
      781 tccttggaca atacttccag cgtaaatgta ttgatcaatg tcatcgttgc caccacgatg
      841 aattacttcc ccgtattctt caaatttcac aaaatcagtg gttgtaacta gtgaccaccc
      901 aaaaggatca gttaaaggtt taggatttct cgtatctcgt tgatgaaata gataaaaagt
      961 tccatcttta taaaaaggca tgcaatcccc aaaccaaaaa tctttaggct tgtaaaaaat
     1021 tttttgcatt tatcactcat cctcttcgtt agtatgtctt tgcatatctg caagacacgc
     1081 aattgtattc atatttctta gattttcgat gatatcatcg cttttttttc cttgtaatct
     1141 caaattgaca gcctcaatca aaattcctaa attcatgcca ccaagaagtt gaaacccatt
     1201 ttgaaatact tcacttcttt tagcaaattc gttgaaaggt gttccaccaa tcaagtcagc
     1261 aaataccata acttcttgat tttttacttg agaaactatt ttttcaatgt tttcacggaa
     1321 ttcttcaatg cctgcatctc caagtgttac cacatgacaa tttaaattat cgccaaaaaa
     1381 catttttaat gtatctacaa atccaatggc aatatttcca tgagatgtaa ttataaactc
     1441 catccactca tcctctgttc ttaataaata aaatccttta aaaattcttt tgaatctcta
     1501 ggagtttgta tcactgtcac ttcaactcca tcactaatta gttctttaaa agcctctctc
     1561 tcttcttcag taatatttat cgtcggacgg atgcgttctg ttccattcct tcttgataaa
     1621 tttcctacgt ttacacttgt tattggtaaa ccgttatcaa tcaaaaattt aacatcgatt
     1681 ggagaaactg caacaataag aactttttgt ccttcatatt taccagaaag aatattttta
     1741 actgctactt cccttgtaat tattgaagtt ctaacattag gtggtgcagc gaggcgaact
     1801 acagcttttt ttacgtcatc attggctact tcattattaa taaccataat tctgttagca
     1861 ctcaattgat ttgtccattg tgttgctact tgtccatgaa tcattcgatc atcaatacga
     1921 acatgtataa tacctttcat tttttcatcc atattcaaca cctacttcaa aatatttaac
     1981 gctcttaacg caatagaaac tactaagatt aatagaatta cataaactgg tttaaccttt
     2041 cgtcctaaca aataatacac aattgctact acacatgcag gtaataaacc aggcataact
     2101 tgatcaaaga tttcatttcc ggtcattgtt aagtcgcctt gtttaaattc aaagccaaca
     2161 ttcactttta caactgtggc aatgagtgca ccgaccacca ttaaccctaa aattgatact
     2221 gcatcagtaa tagactttaa ttttgtttca atttgagtta gtaggctcgc acccgattct
     2281 ttcccagcaa ggtataaagg ataagcagta atccttaata aaattgatgc cagtatccac
     2341 aaaataattc caacaaaact accttccaat gccatattag ctgcaattgc tccaaaaata
     2401 gtccatggaa taactacaaa tacactatct ccaattcccg ctaaaggacc cattagacct
     2461 gttttcagag cacttactgc ttccaaagac ttattttttt cttctttttc aattccaaca
     2521 ttcataccaa taatgtaagg cgctgtatac ggcgtagtgt taaaaaattg taagtgagct
     2581 aaaacggatt gtttcattcc ttcaggatca tctttatatg tgtccttgat aaaaggaagc
     2641 atagaatacg cataacccaa cccttccatt ttttcataat ttaatgaagt tgcaccattg
     2701 agaatccacc ggaaaattac ttgtttgtcg atttttgtca gtttcttatt catcttcgtc
     2761 aaaacctcct gttgcagctg gtgctacttg agtattattt gatctatttt tatatacgat
     2821 tagtgcgatt gcagcgccaa gtaacgaaat ccctaaaatt ggtatttgga aataaactgc
     2881 caaaacgaaa ccaactaaca aataggcaaa gtaatctttt gtaggtaaat atttaagtag
     2941 cattcctatc ccaacagcag gtaatagtcc ccctgctacc tttaaaccac cggtaaccca
     3001 ttcaggaata taattgattg cacttcccac taacgttggg cctaatatca cagttaacaa
     3061 aacaggaatt ccagatacaa agcttgtatt aattgatgcc aaatattgca tccactctaa
     3121 tttttgataa tcttcttttt gtgcgaattt ttctgccttt tgttgaaaaa aaatgtttgt
     3181 tgaccacttt agaacatcta gttgtacaat taacattgct accggtattg ctaaagttat
     3241 tcctatagaa gcatcctggt tagttgagat tgctaaataa gtaccgatca atgctgctgt
     3301 ttgataatcc ggtactgatg caccaccaaa actagtaata cctagtgtca ttaattgcaa
     3361 agtcccaccg atataaagtc cagtttgaat atctcccata ataagtccgg tcaagaaacc
     3421 tgcaacgaca ggttgggagg ttccgatttt gggcccttcc ttatcccagt taatgaaaaa
     3481 tccatacaac acaactaaaa atgtttgcca aaacgccatt ttgttctctc ctttcttctt
     3541 atacttttat ataagcaatt actgtgccaa ctaacattat ccaataaagc gggaaaatac
     3601 agtaaatatt aattaaatga gcgtctaaat atactttgga caccctttca tttttttaga
     3661 cgctagtatg ctatttagaa aatagtctgc tcaaaaattg tatagattaa ctcatactct
     3721 cctttgggta tttcaataca gtaaatatct ttgatttctg aaaaaaaatc ttttgataca
     3781 ttt
//
LOCUS       DACDID010000180         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDID010000180 DACDID010000000
VERSION     DACDID010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.186"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV236_002466"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV236_002466"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8772627.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IV236_002467"
     CDS             complement(2260..2511)
                     /locus_tag="IV236_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8772628.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IV236_002468"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IV236_002468"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8772629.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IV236_002469"
     CDS             3461..3679
                     /locus_tag="IV236_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772630.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACDID010000181         3682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDID010000181 DACDID010000000
VERSION     DACDID010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.187"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..>3682
                     /gene="nifJ"
                     /locus_tag="IV236_002470"
     CDS             22..>3682
                     /gene="nifJ"
                     /locus_tag="IV236_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8772631.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKM"
BASE COUNT         1181 a          696 c          854 g          951 t
ORIGIN      
        1 tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa tacggcagcc
       61 gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac acccagttcg
      121 accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt attcggtcaa
      181 ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt ccatggatca
      241 ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct tttgatgata
      301 ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt tgctagtcgg
      361 gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat ggctgcaaga
      421 caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga tttagcacct
      481 gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt tgatggattc
      541 cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt agctcctctg
      601 atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa tcatccgact
      661 gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac gatcaacccc
      721 tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa ccgattacga
      781 ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga agtcattgtt
      841 tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa tgcaaacgga
      901 cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga aacattcgtg
      961 caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa agaaccaggg
     1021 gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc tgaacttcgc
     1081 ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc cgaccaaatc
     1141 tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg gtttaccata
     1201 gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct ggatttgaca
     1261 gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac agttggagca
     1321 aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca aggctatttt
     1381 tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt tggagatacg
     1441 ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca cacaccagct
     1501 tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt tttattgaat
     1561 actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa acgttacttg
     1621 gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca agaagtcggt
     1681 ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc tgacatcatt
     1741 ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta tggacataaa
     1801 tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga actgcttcat
     1861 caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat caataaaagg
     1921 aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg aaatcaactc
     1981 tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt aggaacggct
     2041 tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga tcgctgtacg
     2101 atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt tttagcagat
     2161 gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg aggcgcagac
     2221 ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg tctgtgtgta
     2281 gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga acagaaagaa
     2341 caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc agtcaaagga
     2401 aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc gggcgcttgc
     2461 tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg tgatcgaatg
     2521 atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc tgcttctcct
     2581 tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt ggaagataat
     2641 gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc tttggcaagt
     2701 tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat ggaagactgg
     2761 ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact gcaagctgcg
     2821 atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag aaaggatcta
     2881 ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga cattggtttt
     2941 ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt tttggataac
     3001 gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc ggcaatcgct
     3061 aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat ggcgatgaca
     3121 tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca aacgatcaag
     3181 gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta tacgccttgt
     3241 atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa agaagctgtt
     3301 tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa aaacaagaac
     3361 ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt catgaggaaa
     3421 caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc tttatttgaa
     3481 aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac gggagaatta
     3541 gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt taagactaga
     3601 aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag agctgccaga
     3661 gcagaacgac gtaaaaaaat gg
//
LOCUS       DACDID010000182         3638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDID010000182 DACDID010000000
VERSION     DACDID010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.188"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..766)
                     /gene="truA"
                     /locus_tag="IV236_002471"
     CDS             complement(26..766)
                     /gene="truA"
                     /locus_tag="IV236_002471"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8772632.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(957..1592)
                     /locus_tag="IV236_002472"
     CDS             complement(957..1592)
                     /locus_tag="IV236_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8772633.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1739..2980)
                     /gene="sstT"
                     /locus_tag="IV236_002473"
     CDS             complement(1739..2980)
                     /gene="sstT"
                     /locus_tag="IV236_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8772634.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3178..>3638
                     /locus_tag="IV236_002474"
     CDS             3178..>3638
                     /locus_tag="IV236_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772635.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGF"
BASE COUNT         1195 a          761 c          567 g         1115 t
ORIGIN      
        1 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
       61 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
      121 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
      181 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
      241 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
      301 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
      361 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
      421 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
      481 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
      541 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
      601 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
      661 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
      721 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
      781 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
      841 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
      901 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
      961 tgtttgagat gcaagatact caacctggca atgggcacat aattaaagaa aaaactgatt
     1021 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     1081 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     1141 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     1201 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     1261 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     1321 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
     1381 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     1441 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     1501 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     1561 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     1621 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     1681 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     1741 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     1801 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     1861 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
     1921 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     1981 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     2041 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     2101 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     2161 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     2221 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     2281 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     2341 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     2401 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     2461 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     2521 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
     2581 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     2641 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     2701 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     2761 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     2821 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     2881 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     2941 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     3001 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     3061 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     3121 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     3181 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     3241 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     3301 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     3361 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
     3421 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
     3481 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     3541 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
     3601 ttgctttttt gtagtattcc ttatacagtt ggattttt
//
LOCUS       DACDID010000183         3585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDID010000183 DACDID010000000
VERSION     DACDID010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.189"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IV236_002475"
                     /pseudo
     CDS             75..470
                     /locus_tag="IV236_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IV236_002476"
     CDS             790..1104
                     /locus_tag="IV236_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772636.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IV236_002477"
     CDS             1208..2386
                     /locus_tag="IV236_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8772637.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IV236_002478"
     CDS             2789..3082
                     /locus_tag="IV236_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8772638.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1221 a          523 c          707 g         1134 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgc
//
LOCUS       DACDID010000184         3564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDID010000184 DACDID010000000
VERSION     DACDID010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.190"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..521
                     /locus_tag="IV236_002479"
                     /pseudo
     CDS             1..521
                     /locus_tag="IV236_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            663..2366
                     /locus_tag="IV236_002480"
     CDS             663..2366
                     /locus_tag="IV236_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8772639.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQ
                     HNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
     gene            2427..>3564
                     /locus_tag="IV236_002481"
     CDS             2427..>3564
                     /locus_tag="IV236_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8772640.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQL"
BASE COUNT         1382 a          593 c          767 g          822 t
ORIGIN      
        1 aagaaaaaga accactagat aatagtcgtt tgggaaagtc caaaagaaaa ttagtacggt
       61 tgcaaaacga attaaataat caatttcaaa agctatttga tcaccaaaaa ttgacaaatg
      121 ggcaaccaat gaatgacaaa cgaaatggtc gttcatggtt taaccagcaa gaaaaaatag
      181 aaaataaaat acaaagtttg agagaagaga tcaagcaaca agaaaaacag gtagaaaaac
      241 ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc ctggacatga
      301 cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcga
      361 ctttttcagc tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta gaacagctga
      421 aagaaaaatc agagaaagga aaagaaaatc ttcttcctga agtacaagcg atcattgaca
      481 gcggacgagt gacgcaatgg aaaaagaatc caactatcta ttttctgaaa ggctatcgaa
      541 aagtcgcttt agaactcaat gaaaacgggg gatttgagga atctaaaaaa taccaagcca
      601 cgtcggaaga agaaaaggca gctgttcgtg aattacttga agaagctaca cctactgatt
      661 ctgtgaagga gaaagaaacc gatcatttct atcgagtaga attcaacgaa aatgatccag
      721 caaaagggtt gaaaagttat ggtggggaga tcgtaacact agaattgatc gatcaactca
      781 aaacactgga taaccaatac gccgcagaag caggctatta taaattctat tttgaagaag
      841 tgaagaatgg agaagtagtc gatcagatac gaatggatct aggggatggc gtcaatagta
      901 atcaccccat ctatttagag ttagctagat tgtgcgaaca atcacctgaa aaggaacaag
      961 aacaaacagt caaaaatgaa cctatttcaa aagtacaaaa cgagattaaa gagacaaaaa
     1021 caaaacaaaa gggttatcaa aaacgaacgg cagaagagat taagcaagaa gtcaaatcac
     1081 tttcccaaca ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa gacgtcaaag
     1141 aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgatcg
     1201 aggaacaatt tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa aaagaaggaa
     1261 tctatatcaa aaaaggagaa caagggaaca agatctttgt cagaaaaacg gtcaaaggat
     1321 tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa gataaaaaac
     1381 gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt gaaaaagtgt
     1441 ttgatatcac acaaacacaa ttgaaacctg aagactatcc gaaaatcttc ccgaaccgcg
     1501 tctttgattt tcaattggat caaacagggc agacagaact tcaagttggg attcaagcgg
     1561 tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc tatcaaagag
     1621 aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa gaagaaatcg
     1681 tcatgaatac tcgaaatact cctacgcagc atttagccac aagtattcac gaattagccc
     1741 atgctagaat gcacaaattt tcagaattag atactgcaac aaaagaacta caggcagaaa
     1801 tgacctccta tattgtgtgc aagcattttg ggatggacac ctcggagaaa gctattcctt
     1861 atattgcgac atggacgaag aatagtcaga cgttggatga taaggaagct gcggaacgtg
     1921 ggaaaatcat gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc atttctaatg
     1981 aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg aagcaggagc
     2041 ataaaaatac tgtagaaacg gcagaacaaa agaatattgt gcccgttggc tcactttgga
     2101 tcgacaagaa agacgggaaa gtcctggaag aagacacggg ccgaacgtta catttgaagg
     2161 atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga aaaagttatg
     2221 gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat ccattccgat
     2281 tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat tatgaacaac
     2341 aattgtcaca aaaagaacgc gtataaaaca agcaagaaag actatctgaa aaatggatag
     2401 tcttttttct tttgggaggt ttgaaaatga cagagaaaaa aatgagtctc attgatcgat
     2461 gcaagcaaat ggatattgtg gattttgcac gaaacaatgg tttagcggtg gccaataaag
     2521 gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga aaacaacgtt
     2581 tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa ctttttttca
     2641 tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat tttattctaa
     2701 agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa aagtacaaag
     2761 atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg aaggaagaac
     2821 gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatt gccgaactaa
     2881 agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttggat catgcggtcg
     2941 cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat caaggaacgt
     3001 tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat aaaaattcga
     3061 cagcgaatca tggctttaac ctaaagattg gtgatcctaa acagttaaaa ttttttgaga
     3121 gtagtattga tttacttagc tacgctgcac tgaatcgtga tcagttaaac gatacgtggt
     3181 tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt gaggcagtat
     3241 ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg gataatgacc
     3301 gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat ccatttacga
     3361 atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca agagaataca
     3421 aagtcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct attatggcca
     3481 tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct cacaaagtca
     3541 atgcgtcttt tggtcagcag cttt
//
LOCUS       DACDID010000185         3528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDID010000185 DACDID010000000
VERSION     DACDID010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.191"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            109..1875
                     /locus_tag="IV236_002482"
     CDS             109..1875
                     /locus_tag="IV236_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8772641.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1890..2327
                     /locus_tag="IV236_002483"
     CDS             1890..2327
                     /locus_tag="IV236_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8772642.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2340..3491
                     /locus_tag="IV236_002484"
     CDS             2340..3491
                     /locus_tag="IV236_002484"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8772643.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1183 a          631 c          766 g          948 t
ORIGIN      
        1 ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt
       61 agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg
      121 attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca
      181 agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt
      241 atcccagatg gttatttacc aaatgaggct ttggctacca tggtaagccc aatgctaact
      301 tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga
      361 tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg
      421 ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta
      481 ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt
      541 atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg
      601 actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca
      661 agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt
      721 ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt
      781 gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga
      841 gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa
      901 atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga
      961 atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca
     1021 gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg
     1081 ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat
     1141 gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa
     1201 gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc
     1261 atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa
     1321 aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca
     1381 gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa
     1441 gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa
     1501 atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca
     1561 gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat
     1621 gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac
     1681 catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca
     1741 ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt
     1801 catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg
     1861 aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc
     1921 tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag
     1981 aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat
     2041 ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg
     2101 tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag
     2161 aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc
     2221 aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag
     2281 acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa
     2341 tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg
     2401 cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa
     2461 atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa
     2521 aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg
     2581 aagcagattt agttactacc gccattggcc cgaatatctt accttttatt gctgagttga
     2641 ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg
     2701 cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc
     2761 cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg
     2821 tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg
     2881 tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag
     2941 atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg
     3001 tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt
     3061 tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat
     3121 gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa
     3181 acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt
     3241 atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac
     3301 atttgacggc aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc
     3361 gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag
     3421 gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc
     3481 aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaattttt
//
LOCUS       DACDID010000186         3528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDID010000186 DACDID010000000
VERSION     DACDID010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.192"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..599)
                     /locus_tag="IV236_002485"
     CDS             complement(<1..599)
                     /locus_tag="IV236_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8772644.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIA"
     gene            760..1449
                     /locus_tag="IV236_002486"
     CDS             760..1449
                     /locus_tag="IV236_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8772645.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1463..2965
                     /locus_tag="IV236_002487"
     CDS             1463..2965
                     /locus_tag="IV236_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8772646.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2978..3454
                     /locus_tag="IV236_002488"
     CDS             2978..3454
                     /locus_tag="IV236_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8772647.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1159 a          498 c          649 g         1222 t
ORIGIN      
        1 gcaataattt ttgcgccatt ttttttagct attttcaatg aatcataagt atctttcgtt
       61 ttcccagata aagaaaaaat gattactaaa tcattaactg ttaataaaga agcgacttgg
      121 gcttgaaaat gagggtctag tacagctttt gcttgaactc ctacgcgaag aaacatattt
      181 tctaaatcaa gactagtatt tccactagat cccactccaa atatatatat gttttttgat
      241 tgagtgatca ttttaatggc ttcattcaat ttttcttcgt ctaaaagcct aatagtggaa
      301 tagagagctt ctgttaaact attagcaatt tcatcatagt actttacact agtcggatgt
      361 tctttttttt gactaaagtc ttcctttgca atttcaattt ttaaatcaga aaaaccagaa
      421 aaaccaattt tttgacaaaa gcgaataata gtagcatcgc caacttttgt gtttttctta
      481 atatcactca tcgtactata aataatagtt ttcccagaat ttaaaataaa atcagcgact
      541 tttttttcag acttcgttaa aaatggataa ttttctttaa ttaaatagat ataactcata
      601 caaactcctc tacaatttca acttttctcc attatactcc aaaataataa aaaaaactcc
      661 taaatactcc atttttatct ttacttttat gaaagcggat gctatattgt ttttggagtt
      721 gttatccaaa aataataaaa aattggagtg tttaggataa tggataaaaa gaggttttta
      781 gatcttattt caaaaggttt gattgtttcc tgtcaagcat tacctggtga accattttac
      841 aaagaggaag aaagtttaat gccttatttt gctttggctg ctcaaagggc tggagcagtt
      901 ggtatacgtg caaattctgt tcgagatatt attgaaatta gaaagtgtgt atcattacca
      961 atgattggaa ttattaaaag agattatcca ccagaatctc cattcattac tgccactatg
     1021 agagaagtag atgaattagt agaaaccggt gttgaggtaa tcgctcttga ttgcactctt
     1081 agaaatcgac atgatggact atctataaat tcttttatta ctaaaataaa agaaaaatat
     1141 cctcatcagt tatttatggc agatatttct acatttgaag aaggcaaaaa tgcatataaa
     1201 gcaggaatag attttgtagg aactacttta agtggatata ctgaggaaag taaaaaacaa
     1261 gatgggccag atatagattt gattaaaaaa ttggtagaag aagggatttg tgtaattgct
     1321 gagggaagga ttcatactcc agaacaagca aaagagataa aaaaaatagg agttgcaggt
     1381 attgttgtgg gtggtgctat tactcgacca caggaaattg catcaagatt tgttaaagca
     1441 ttaagttagg gggagaaata aaatgtttaa aaaattttca caattaggac gcgctttcat
     1501 gttaccaatt gctatattac cagtagccgg attactattg ggactgggag gagcgttaac
     1561 aaatgagagt gctataaatg cttatccatt tttagatcaa ccgtggttgc atacaatttt
     1621 aagtattatg agttatgctg gaaatgcagt ttttgcaaac ctagcactaa tttttgcaat
     1681 tggtattgct gtaggacttg caaatggaga taaaggaaca gctggtttag ctggtggagt
     1741 agcttatcta gtatatactg caactattag tgggtttctt gaactttttt cagcaaaaga
     1801 tgcaacacta gatacaggtg tagtgggatc aattgttgtt ggtggtactg ttgcattttt
     1861 acacaatcgt tatagaaaaa ttgaattacc acaattctta ggtttttttg gaggatcaag
     1921 atttattccg attgtttcta gtttagctgc aatcattatt ggttctttct tttatttaat
     1981 ttggccacca attcaaaatg gtttagtagt tgctggtgaa caaattgccc aaatgggtag
     2041 tttaggaact tttttatatg gttttttatt gcgtttaaca ggtgctgttg gactacacca
     2101 caccatttac cctttgttct ggtatacatc tttaggtggt accgaaactg tagctggatc
     2161 aacaattgct ggagcccaaa atattttctt tgcccaacta gctgatccaa accacactgg
     2221 tctgtttact tatggtacta gattttttgc aggaagattt gcaacaatga tgtttggatt
     2281 accagcagca tgttatgcaa tgtatcgtgc aattcctaag aaaaatcgta aaaagaatgg
     2341 tgggctttat tttagcgggg cccttacatc atttttaact ggaattactg aaccagttga
     2401 atatatgttt ttatttgttg ctccttggct ttatgtgatt catgcattct tagatggatt
     2461 atctttttat tttgctgata tcttaaatat tcgtgtaggg aattcctttt caggtggttt
     2521 gatcgacttt ttattattcg gtgtacttca agggaatgac aaaactaatt ggttaaaatt
     2581 aattcctttt ggtattgcat gggcatttat atatttcttt gtttttacat tctttatcaa
     2641 aaaatttaag gtagctattc ctggaatgga agaagaatca gaagttcatt ctgaaaaagt
     2701 aataaacaaa aatacatcat tacatgaaga gtctcttatc attattgaag ctcttggtgg
     2761 aaaagaaaat attgagtctg ttgaagcttg tgcaactcgt ttgcgtgttg ctgttacgaa
     2821 tggggagatt attcaaaaac aaacaataaa agacactggt gcaactgctg tcttcgaagt
     2881 gaagggtggc atccaagctg tttttggagg gaaagctgac ttattaagtc aagaaatcaa
     2941 tcaaatatta ggaaaagata attaggaggt ttttaatatg tttgaaattt tcaaaaagaa
     3001 aaaacaagaa actttttatg caccttgttc cggaaaaatc atttccatca cagaagtaaa
     3061 tgatcctgtc ttttcacaaa aattactagg tgatggtttt gccgtcatac ctgatggttc
     3121 aaaaatttat gctccattgg ctggtcgagt aacaaatata tttcctacta aacatgctat
     3181 taatataaaa agcaactcag gcattgatta tttgattcat attggaattg atacagttga
     3241 attacaagga gagccttttt cgatttgtgt tcaagaaaat gatgaggtta ctcctgaaac
     3301 ctcgttggtt gatgtggatt ttgacaaaat aataaaatct ggtaaagata cttctgttat
     3361 agttgttttt ccggagaaaa atcagttcaa tgatttaata atagataatg gtcgaattgc
     3421 tcatgggaat tcatgtggaa aaatcacttt ttaaaaaagt gtatataaaa agctataaaa
     3481 taagctaaat aggatatttg tcaaatgaag ttggtgaaca gaaagaac
//
LOCUS       DACDID010000187         3521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDID010000187 DACDID010000000
VERSION     DACDID010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.193"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..993
                     /locus_tag="IV236_002489"
     CDS             19..993
                     /locus_tag="IV236_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8772648.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            975..1436
                     /locus_tag="IV236_002490"
     CDS             975..1436
                     /locus_tag="IV236_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8772649.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1486..1950
                     /locus_tag="IV236_002491"
     CDS             1486..1950
                     /locus_tag="IV236_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8772650.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1964..2713
                     /locus_tag="IV236_002492"
     CDS             1964..2713
                     /locus_tag="IV236_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772651.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2713..>3521
                     /locus_tag="IV236_002493"
     CDS             2713..>3521
                     /locus_tag="IV236_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8772652.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFV"
BASE COUNT         1175 a          477 c          688 g         1181 t
ORIGIN      
        1 agagtgaggg ataaagaaat gagacaacta cacaaagaac agattgaaac agcaatggca
       61 gcgttaaagg aaaagacaat tgatcatgta tattttacag catgtggggg ttcacaagcc
      121 gttttaatgg ctggtcaata tctatttgat aaagaattaa ctgtaccttg tcatgtttat
      181 actgctaacg aatttaacta tgctatacca aaaggattat ctgaaaattc tttagttatc
      241 acttgttccc attctggcac aacaccagaa actgtagaag caacaaaaaa agcaagagaa
      301 tttggagcaa tgactatttg tttatctaat gaagaaggat ctcctctatg ggaagcagct
      361 gaatatcctg ttcactatga ttggggtaaa gatgttgatg ctagtgattt aaataaagga
      421 attctttatg gtttattatt taatatttta cttgttttag atggctctaa aaaatgggaa
      481 acttgcttga atgaatttga taagttaaat gatcttgtta ttgctgaaaa gagcaaattt
      541 gaaataactg ctaaagaatg gggtaaaaac caaaaacgag ataaaattat ttacacaatt
      601 ggtagcggta tcaattacgg agaaatttac tccacagcag cttgctggtt catggaaatg
      661 caatggatta attctaatgc tattcattct ggggaatttt tccatggtcc ttttgaaatc
      721 actgattatg atgtaccatt catcttagtg aaaagtattg gagatacaag atttttagat
      781 caacgggtag aagacttcac taaaaaattc agtaataaat taatggtttt agaccaagct
      841 gattttgact tagaatctat agatagtgta gcgaaagaat atattgcagc tgtacttagt
      901 ggcgttgtta ttcgccaaat ggtagaagcg attgcttttg aaagaggcca ttccttagat
      961 gttagacgct atatgtggca aatgaaatat taattaatag gaggttgcaa aagatggcaa
     1021 atcaattatt tcacattgtg attacaacac attctaccct ttgtgagggt tatttaaagg
     1081 caacaaaatt aattttaatg aatgacgcca caggtattag tgctgttcca tttaaagaaa
     1141 atactagtcc agatgatttt gaggagagta tgaaagaaat aattagtcag catcatttgc
     1201 aacctctaat tattttaacg gatttaattg gaggaacacc taataatgtt tctattaaac
     1261 agttgaacaa caatagaatt caaattattg ctgggattaa tttacctctt ttactggagt
     1321 tattaatggc ccagtcagct ggtaaatcta tagaagaaat agattttaag gaattactga
     1381 ataactctca aaaaagtatg gttcatatta attcgctttt aaaagatgtc agttaaggtg
     1441 gcaatttcta ttctataaat tttaaaaatt gaaggaggaa atagaatgat taaattagtg
     1501 cgtgtagacc acagattact tcatggacaa gtaatatttt cttggactaa acagtatgat
     1561 attaaccata ttattgtggc tgataacaaa gttccaggag atccaatgtc tgtgatggct
     1621 ttgacaattg ctaaacctgc tgattgcaaa ttagacattg ttaaaattag tgatgttaaa
     1681 gggattatgg aaacatcccc aaatgataat tacatgattt taattaaagg acctaaggaa
     1741 gcttttgaat tagttcaaaa tttaccagaa attaaagaaa ttaattttgg cggtgtgtct
     1801 aagaaaagcg agtccaaaca atatggaaag gcagtctttt taactcctga agaattagaa
     1861 gccacaaaaa aaattcttga tcaagaagtg aaaatatttg tgcaacaagt cccatcttca
     1921 agtattgaaa atgtagattt tagtaagtaa ggggggaaaa taaatgttat taaaagccat
     1981 tttattaggt ttagttggtg tggtagctgt tattgactcc aggttaattg gtcgacaaaa
     2041 tattggtaga ccactaattt tgagtacact tgccggactt gtgctaggtg acttgcatac
     2101 agggattgtt cttggggcat ctttagaatt aatttctatg ggattcgtct caattggggc
     2161 ggctggacct cctaatatgc aacttggtag tattattgcc actgcttttg caattttaac
     2221 taatagtagt acagaaacag ccttaacaat agctatccca gtggctgtag ctggtgaatt
     2281 tatgagtatt atcatgcgca tgataattgc tcaatttgga catgctgcag ataaagccat
     2341 tgaaaataat aaatttagaa aagctcaaat tattcatatt tattggtcat ttatttttaa
     2401 tgcttttgtt tactttattc caatcttttt aacagtatat tttggtgctg acgttgtggc
     2461 agatttagta gataaaattc cagaaatgat tacagacgcc ttgactgtct ctggaaatat
     2521 gctttccgca ttagggtttg caatgctatt aagcacaatg ttgagtaaaa aattatatcc
     2581 ttactttatt tttggattct ttattgttgc ctattcaggt ctttcactaa ttggcgtgac
     2641 aatttttgca gctctgattg cctttgtaat ggatcaagtt aaatatggaa aaagagaaga
     2701 ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt taatcacaaa aaaagatctt
     2761 aataagatgt gtcttcgttc atggacaatc atgtgttcgt ggaattatga aagacaaatg
     2821 catatgggat ttatgtatgg gatggcgcca attttaaata aagtttataa aaatgatgaa
     2881 gagaaaaaaa gagaagctta tactcgtcat atggcatttt ttaactgtac gcctcaaatg
     2941 acttcattta ttatgggact ttgtgcttct atggaagaac aaaatgctca agcagaagaa
     3001 ggaaacttta atactgaatc tatctccatg attaaaacca gtttaatggg tccttttgct
     3061 ggaattggtg attcattttt ccaaggtact ttaagaatta ttacatttgg gattggattg
     3121 tcttttgcta agcaaggtag tattttagga ccgattttag ctgttggcct ttttgctata
     3181 ccttctattt tagctgtttt ttacggcggt aaattaggat atgagtctgg caataaatac
     3241 cttactaaat tgtatcaaga aggtcttatg gatcgagtaa tgcaatttgc ttctatcatt
     3301 ggtttggctg tagtaggtgg aatggtggct agcatggtgc caattgtcac ccctttaaaa
     3361 ttcactacag ggggaacaga acttgtttta caagacttac ttgattccat tattcctgga
     3421 ttattaccac ttgtatgtac tttgggaata tataaattga ttaagaaaaa tgttagtaca
     3481 aatatgctct tattaggttg tattttggca gggtttgttt t
//
LOCUS       DACDID010000188         3470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDID010000188 DACDID010000000
VERSION     DACDID010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.194"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(445..1419)
                     /gene="sgrA"
                     /locus_tag="IV236_002494"
     CDS             complement(445..1419)
                     /gene="sgrA"
                     /locus_tag="IV236_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8772653.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1615..2454)
                     /locus_tag="IV236_002495"
     CDS             complement(1615..2454)
                     /locus_tag="IV236_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8772654.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2639..>3470
                     /locus_tag="IV236_002496"
     CDS             2639..>3470
                     /locus_tag="IV236_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8772655.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     V"
BASE COUNT         1151 a          720 c          514 g         1085 t
ORIGIN      
        1 ttgaatcacc gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac
       61 cacatataat ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc
      121 attgatatca ataaattctt caaacaaagt acatactcct ttttatctat tatttagact
      181 tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac
      241 taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt
      301 tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc
      361 gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa
      421 ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct
      481 tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct
      541 actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg
      601 atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc
      661 atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt
      721 tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt
      781 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact
      841 ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc tactttcact
      901 gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt
      961 gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt
     1021 gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc
     1081 atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac
     1141 gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata
     1201 tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt
     1261 aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt
     1321 ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag
     1381 cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat
     1441 taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt
     1501 tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa
     1561 tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg
     1621 ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac
     1681 aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc
     1741 agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc
     1801 gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat
     1861 agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa
     1921 gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa
     1981 tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa
     2041 gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat
     2101 cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg
     2161 actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca
     2221 gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc
     2281 aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat
     2341 ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac
     2401 aaaaccaact aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca
     2461 tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa
     2521 atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta
     2581 cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat
     2641 gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact
     2701 attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac
     2761 agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa
     2821 aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt
     2881 aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa
     2941 agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca
     3001 atacggtttg aaagccaatg taaaattaac agataaagat ctagacactg cttggaaaga
     3061 attctatcca gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa
     3121 agcaaaaaaa gaagcaaatg aaggggaaaa tttctccaag cttgttcaag cttatggaaa
     3181 aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat
     3241 tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat
     3301 tcctgtaaca gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa
     3361 acaggataaa ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga
     3421 agcaaaaact cttgatacgg aattcatgga taaaacaatc cgtaaagtga
//
LOCUS       DACDID010000189         3464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDID010000189 DACDID010000000
VERSION     DACDID010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.195"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..897
                     /locus_tag="IV236_002497"
     CDS             10..897
                     /locus_tag="IV236_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8772656.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            894..2063
                     /locus_tag="IV236_002498"
     CDS             894..2063
                     /locus_tag="IV236_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772657.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2087..3442
                     /locus_tag="IV236_002499"
     CDS             2087..3442
                     /locus_tag="IV236_002499"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8772658.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         1107 a          639 c          826 g          892 t
ORIGIN      
        1 aggcttttta tgtcatttca aatcggggaa ctttttaatt tggactattg gcagcaaatg
       61 ttttccggtg acttgttctc tctgaatttt ttggtgaaca tacttgatat tctggttgtc
      121 tggtatcttg tatacaaact gatccaactg ctgcgaggca caaaagcgat tcagcttctg
      181 aaaggtgtag ctatttttat tgttatccga atcgtagcgg aaatcattgg tctgcacacc
      241 ttgtcttggc tgatgaacca agtcattacg tacggggtga tagctgctat cgtgattttt
      301 cagccggaaa tccggagagg actggaacat cttggacgca gttcgctttt caaacaatca
      361 aaaagcgaac agcaggaaga tgagaagatg atcttgtctt ttgacaaagc catccaatat
      421 atgtccaagc gaaaaatcgg tgcgttgatc actatcgaac gtcatactgg attagatgag
      481 tatattgaga cagggattgc tttagatgca gatatcactg gggagctgct gatcaatatc
      541 tttattccta atacaccgct gcatgacggt gcagttattg taaaagaagg aaagatcgca
      601 gtagcaagcg cctatcttcc tctatcagaa agtatgttga tccctaaaga attcgggact
      661 cgacaccggg cagctgtcgg tataagcgaa gtcagtgacg cgattaccat cgttgtctct
      721 gaagaaaccg gggacgtcag catcacgtta gataatgaac tgatggctgg cttatcccag
      781 caagaatatt tggcaatctt gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag
      841 aaaaacgtga ttcaatcttt cctagacggt atgactaagg gaggggatcg caaatgatct
      901 caaaagagaa acggacaagc attctttaca gttttattgc attgctgttc agtattgcgc
      961 tgtacttcaa tgcaaatggc cagtccgttc caaatacgct ttctggaaat gaagcgtata
     1021 atcaaacagt tacgggggtg ccgatcaaga tttcttatga ttcaaagcgc tactatatcc
     1081 atggatatga aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt cagttgaatg
     1141 cagaggcaaa cgaagataca cggatgttcc gcgtcacagc agatatcact aagctgggcg
     1201 aaggaacaca tgaagttccg ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga
     1261 tcgaaccatc gactattaca gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg
     1321 aaacgttgat tccttcaacg ttcacaccat ctggatatga acttgatgat acttccgtta
     1381 gtcctaaaaa agtctctatc actacaggtg acaaaacatt ggcagaaatc aaacgagtag
     1441 tagctgatgt ggacgcttcg atggtcacag atgatgggat taatagtgag gtcccaatac
     1501 aggcgctgaa tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg caagtaacag
     1561 tgagagctga ttcgatcaag ccaagcaagt cggtacgact ttatgggata cagcaaggaa
     1621 caccagctgc cggggtagaa agctatgatt tcagtttttc aaaactggaa gcagaagtaa
     1681 gcggctctag cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct
     1741 caggtatcaa tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag gggttaagta
     1801 tcaagcccga atcagttagt gtggagatta cacccgtgtt gcaaacgtca agcagtagct
     1861 ctgcttcgac gagcagtcgg acacaagaaa atacaagtac gagtagaagt atttcatctg
     1921 ctgattcagg aacggaacca tcgacaacaa ccatttcttc cagttcggaa tctgggacaa
     1981 gcagctcgac cacaagtaca gaagaaacat cagaaacaag cgaatcaaca caaatgagca
     2041 gaacaaattc ctcagaaaat taagttaaag gagtaatttt acaactatgg gtaaatattt
     2101 tggaacagac ggcgtacgcg gtgaagccaa caaggaactt acgccagaac ttgcatttaa
     2161 gctgggacgg tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg
     2221 cgtgttagta gggcgggata caagaatctc aggtcaaatg ttagaagaag cgctgatttc
     2281 aggactttta tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt
     2341 tgcttatctg acacgtttgc aaaaagcaag cgcaggggtc atgatttctg cttctcataa
     2401 tccagcacaa gataatggaa tcaaattttt cggtgcagat ggttttaaac tagtagatga
     2461 ccaagagcta gaaattgaag cattattaga tgctgaagtg gatgaattgc ctcgtccatc
     2521 agctgaagga ttgggaactg tagaagaatt tccagaaggc ttattgaaat attctcaatt
     2581 ccttgtacaa acgatcaacg gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa
     2641 cggagcaact tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag atttttatac
     2701 aatgggcaca aatccagacg ggttaaatat caatgacggc gtaggctcta cccatccaga
     2761 acgtttagct gagatggtcg ttgaaaaagg agcagatgct ggactagctt tcgatggcga
     2821 tggcgatcgt attattgcgg tggatgaact tggaaacatt gtcgacggtg ataaaatcat
     2881 gtttatctgt gctaaatact tggcagaaaa aaatcgattg aaaaaagata cgatcgttac
     2941 gactgtcatg agtaacttag gattccacaa agcggtggaa ggaatcggtt tgaaagatgt
     3001 cgttacacaa gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg attataactt
     3061 tggcggcgag cagtctgggc acatgatttt cttagatttt aacacaactg gagatgggat
     3121 gctttcagga attcagttac tgaatatcat gaaacagaca ggtaagaaat tatcagagct
     3181 tgcagcagag gtaacaatct atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa
     3241 cggtgcgatg gatgttccgg caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg
     3301 atcagaagga agaatcctag ttcgtccttc cggtacagaa ccattacttc gagtgatggc
     3361 agaagcgcct actgatgaga aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa
     3421 agaagaaatc ggactggatt aagagatatt ttcataaaaa ataa
//
LOCUS       DACDID010000190         3445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDID010000190 DACDID010000000
VERSION     DACDID010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.196"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..265
                     /locus_tag="IV236_002500"
     CDS             <1..265
                     /locus_tag="IV236_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772659.1"
                     /translation="FGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQ
                     NHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            279..968
                     /locus_tag="IV236_002501"
     CDS             279..968
                     /locus_tag="IV236_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772660.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1093..3444
                     /locus_tag="IV236_002502"
     CDS             1093..3444
                     /locus_tag="IV236_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8772661.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1155 a          455 c          733 g         1102 t
ORIGIN      
        1 atttggtgat ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga
       61 taaaattgtt gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt
      121 tagttataaa caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc
      181 gattaaggat ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga
      241 aactaattca gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt
      301 ctgttgggct acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag
      361 ttatttcaga tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata
      421 aagtacttgc attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg
      481 ataatccaga tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag
      541 tgacagaagc aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa
      601 ttatgcctcg aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag
      661 caggaggagg aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta
      721 tgggtgtaaa accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg
      781 atggaagagt aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa
      841 tggcattgta tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa
      901 tgaatatagg tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa
      961 atatttaata aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg
     1021 acaaacacta aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag
     1081 caggggattt ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac
     1141 agtaatgtta cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc
     1201 tatggactta ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt
     1261 ggacctacca ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta
     1321 cctaaaaata ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt
     1381 ggcttcgatg gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat
     1441 gcagggttta atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta
     1501 tttgatatga gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag
     1561 caatggttaa atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat
     1621 caaagaaatt caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc
     1681 agtgaagcaa cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa
     1741 ggtaactcgg gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt
     1801 gaagacggta cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc
     1861 cgaaaattta tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga
     1921 ctattgtcta gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa
     1981 ttaactagat ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat
     2041 cttactggta ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa
     2101 ttgaataata ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca
     2161 gcattagctt attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca
     2221 gcaaaaaatc taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg
     2281 ttaggggaag aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg
     2341 tcatatagcg ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc
     2401 aataacgggt atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat
     2461 ttaggttttc ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga
     2521 agtggcaacg gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt
     2581 aatgagttat caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt
     2641 ataaatcaga tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg
     2701 aaccctcatt tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt
     2761 tcttctcctg taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact
     2821 aatagcgagg gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg
     2881 cctcacttaa ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt
     2941 gtttctgact ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa
     3001 ttaggaatac aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca
     3061 tttagtctag aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa
     3121 agaaattctg attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct
     3181 attgatagat ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca
     3241 gtaacaaata gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt
     3301 tcatttttca ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat
     3361 tttattggag atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa
     3421 gaaaatagaa gtaaacttaa ttaag
//
LOCUS       DACDID010000191         3440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDID010000191 DACDID010000000
VERSION     DACDID010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.197"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..899
                     /locus_tag="IV236_002503"
     CDS             9..899
                     /locus_tag="IV236_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8772662.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(972..1796)
                     /gene="nadE"
                     /locus_tag="IV236_002504"
     CDS             complement(972..1796)
                     /gene="nadE"
                     /locus_tag="IV236_002504"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8772663.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1808..3280)
                     /locus_tag="IV236_002505"
     CDS             complement(1808..3280)
                     /locus_tag="IV236_002505"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8772664.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          971 a          725 c          618 g         1126 t
ORIGIN      
        1 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
       61 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
      121 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
      181 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
      241 ataaaatcga tattagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
      301 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
      361 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
      421 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
      481 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
      541 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
      601 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
      661 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
      721 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
      781 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
      841 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
      901 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
      961 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
     1021 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
     1081 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
     1141 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
     1201 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
     1261 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
     1321 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
     1381 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
     1441 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
     1501 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
     1561 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
     1621 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
     1681 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
     1741 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
     1801 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
     1861 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1921 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     1981 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     2041 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     2101 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     2161 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     2221 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     2281 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     2341 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     2401 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     2461 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     2521 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     2581 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     2641 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     2701 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     2761 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     2821 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     2881 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatca
     2941 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     3001 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     3061 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     3121 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     3181 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     3241 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     3301 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     3361 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     3421 aaactatcct ttcttattgg
//
LOCUS       DACDID010000192         3378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDID010000192 DACDID010000000
VERSION     DACDID010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.198"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..249)
                     /locus_tag="IV236_002506"
     CDS             complement(<1..249)
                     /locus_tag="IV236_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772665.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDR"
     gene            complement(209..1963)
                     /locus_tag="IV236_002507"
     CDS             complement(209..1963)
                     /locus_tag="IV236_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772666.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(2073..2642)
                     /locus_tag="IV236_002508"
     CDS             complement(2073..2642)
                     /locus_tag="IV236_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8772667.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(2660..>3378)
                     /locus_tag="IV236_002509"
     CDS             complement(2660..>3378)
                     /locus_tag="IV236_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8772668.1"
                     /translation="ALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVK
                     VGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTES
                     VRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLF
                     LFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKD
                     IKLRVEDSTLIEQIFNDITK"
BASE COUNT         1144 a          639 c          441 g         1154 t
ORIGIN      
        1 cctatctaac aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca
       61 acggggcttt gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg
      121 taccatatta atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc
      181 gccctgatca cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat
      241 catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt
      301 cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag
      361 atttaatgct ggataatcga tgagctatca taataactgt tttttctttc atcagatgtt
      421 gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca
      481 taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc
      541 ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt
      601 ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt
      661 gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa
      721 agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac
      781 caattttaat ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac
      841 tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt
      901 tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt
      961 cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat
     1021 aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca
     1081 ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa
     1141 cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa
     1201 tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag
     1261 aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg
     1321 ttactttagc accaaatatc tttataactt gaattccgcg gatatattct accgtttcag
     1381 agcttaacac atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt
     1441 tctttaacaa gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt
     1501 taatgaaaaa gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg
     1561 gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa
     1621 tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc
     1681 ccttttttct caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa
     1741 aaatataaaa taaagctcct aaagttaacc aagcaacagt ttgaaccgca taactcttag
     1801 ctaaatattc atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc
     1861 catagactgt taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt
     1921 tcattttagg gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat
     1981 tcaatttctt ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga
     2041 gatgtacaaa cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt
     2101 cttccaatca atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc
     2161 catataccta ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct
     2221 atccaatctt gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca
     2281 ctgcctaatt gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat
     2341 acagatagaa taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag
     2401 tacgccccgc ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt
     2461 ccttttctag ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa
     2521 atcccggggg atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa
     2581 aagtaaatta ctagggaaat aactgtagct aaaccaataa aaatataatc ttttgttttc
     2641 atatatattc tcctcaattc tatttcgtta tgtcattaaa aatctgttct atcaaagttg
     2701 aatcttctac tctaagtttt atatcttttt ttattccacc attttttaaa taaataattc
     2761 tattgcatgt tttataaaga aattccggat catgagtagc tataactact gcaccctttt
     2821 cttttaagtc tttaataagg tcagcaatcc tttccatatt aatataatcc aacccacttg
     2881 taggttcatc aaatagaaat aaattagttt ttgacaatga agctatagaa accaataatc
     2941 tttgtttttc tccaccggat aaatcaaacg gtaattgatc tttcatttta taaagttcca
     3001 ttttttttaa aattttttct tgctgatctt tactaactat ttcattcccc agggctaact
     3061 cgctcctcac tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg
     3121 atgacaaatt ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga
     3181 gtattcttag taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc
     3241 caactttgac atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt
     3301 ttaatacatc tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt
     3361 caaatttcct taatgcgt
//
LOCUS       DACDID010000193         3339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDID010000193 DACDID010000000
VERSION     DACDID010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.199"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV236_002510"
     CDS             complement(<1..2232)
                     /locus_tag="IV236_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772669.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IV236_002511"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IV236_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          920 a          746 c          537 g         1136 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaat
//
LOCUS       DACDID010000194         3226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDID010000194 DACDID010000000
VERSION     DACDID010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.200"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(654..1694)
                     /locus_tag="IV236_002512"
     CDS             complement(654..1694)
                     /locus_tag="IV236_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8772670.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2043..2369)
                     /locus_tag="IV236_002513"
     CDS             complement(2043..2369)
                     /locus_tag="IV236_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772671.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2356..3144)
                     /locus_tag="IV236_002514"
     CDS             complement(2356..3144)
                     /locus_tag="IV236_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8772672.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          973 a          549 c          458 g         1246 t
ORIGIN      
        1 tgcttaggca tagattcatc gtaatatcac gtatttttaa ccttaacaca gccaaaaaaa
       61 gtggtatcac ttttgaatat ctgttatact aaatccgtta cgggtaatac cgtaacactg
      121 aaaggtaaac gcattaaggg agtgatctta atgccacctg tggacgtcga cgaaggttac
      181 cagccttccg agtctcacag ggcctttctt ttttttgtat tttcaattgt ccaaatttat
      241 cgtccaaaag tgcagaacga aaatcaattc atcacaagca tagcagacaa ttaaatttaa
      301 aacaactaaa aatatacaaa aaagtgtaat tgaatattag cagcactgcc tgaaaagaag
      361 taatatcagg cttttcagct tggggaaata agtgaattac ctctcttcat gaattattat
      421 aactcaaaaa agcacatatg caaacactag ttccctattt ttttgagtga aaaataacta
      481 aaatatctaa cgattatgaa tttaaaacct acgattgtga ttgttttcat aaacttttaa
      541 tctaaaaact ccggaatact aactatttat ttgtaatatt tgtaataaaa ttaaaatcat
      601 aaaaaagttc taatttaaaa ttgatcattt ttatagtaat tgctttttat caactactct
      661 aaccaatttt ccgtactaac tgttggtaat tcgttaaatg tttctctttg ttcgtttgca
      721 agctttgccg caatagcttt ttcgacaaac caatttttta cataaacgaa aattagattc
      781 tgcaaattgt ttactttttc agttttttgt ttttgataag ctttcagtaa agtcatatat
      841 aatcctttgt cagcatcaat tccataagat tccagttctt cataaacgac aatagtttgc
      901 tctaattctt cagctttttt ctttgcatta tgaatagttt ggacggtttg ttgagcttca
      961 ctgtaacttg aagaaaatgt ttttatcaag tttaatacat tttctggtac aaatgtattt
     1021 atactgtcct cttgcatgat gtcgacgaag ttatctagca aaacttgatt ttgtaactgg
     1081 tcagtttcag tgtctattat gtgtctatta gtgtctatag aattagaata gaatagattg
     1141 tctgcgattt tcgcaattac ggttgagtcc ttgatatgac tggattttgc cgtatttgcg
     1201 attttcgcaa ttacggtaga atcattgatt tttcgcggtt ttgccgtatt tgcgattttc
     1261 gcaattacgg tggaatcagt attgtcttcc tcttccatga ttttaaagac gtcttgattc
     1321 gtaacttctg gcttgcctaa gtacaataaa ttaggcatag attcaatctt accatcacgt
     1381 ttatttactc ttcctctctt ctgtttcaga agacctgctt tttctaattc ttttttgatc
     1441 ttagatactt taccttcgcg gcattgtaac aatttcatta attcttcaac tgtgaatatg
     1501 aagaatatat tatcatcaga atctacccag ttatttttga ctgaataatc aaatcgatct
     1561 ttaagtagca tataagcaat ttttgcatca tttgaaagat ccatgtattt ttcgttagta
     1621 aaaaatacct taggcatttg ataatatttt tcacgataaa tttcttttga tttgtaataa
     1681 ttaaaatcat tcatagccaa tcctctttct gtaggatagc gttttgttca aaactatgtt
     1741 acaatgaatt aaacaaatat atctatccac tatatatttt tctactgctg ataagtcgcc
     1801 aaactttgtt aaagcagtag tcttttttta tcttttttta gtttacctta aaaaataaat
     1861 taaagcaaac cgctctattt cagggtttaa gctcatggta actatatcac gatattacaa
     1921 taacattata tcacgatatc gtaatattac aatatgaaga taagcgactt atcagctttg
     1981 atactactga tttttataaa aaaaatatca cgatattatg atatcgtgat attgaatgta
     2041 aattaaattt tattttgttt ctttgctagt ttatatcctt caatcaagta ttcttcaggt
     2101 atggatttta atagcgcatc ttctataacg tctggtttac ttttgtctga ttcagcaata
     2161 gtcgtccaat atttgagtgc tgacattaga attggattta tacgaatgct atctgctgtt
     2221 cttacactac ttccttgatc ttttatgact ttacttatat ttttcttggt taattcgatt
     2281 tcttctactt cttttggttt gtagttcttt ttatttttat ttcctagtag tctacttact
     2341 gcagcttgac tgcgattatc ttctgccatc aaaatcacca acttctttta aatattcttc
     2401 tgtaaatgct ttatagtcat cttcagctgt tgagggttta ttaaaattga ttagatctga
     2461 tccagcagct tgcgcttcgt caacggttat tgttctacga atataagttt tgaaaatttt
     2521 tgcaccaatg actctagaaa tttcggttgc tgcagtcgtc atttctttag aaatgttata
     2581 acgcttatta tacttagtaa ataggactcc tcgtacttca agcttgtcgt tcgtatattc
     2641 ttttaccgtt tcaatagttt cacttaactt tattagtcct tttaatgaga aatacgcagc
     2701 ttcaactgga atgataattt catcagaaac agtaaatgcg ttgactgtaa gcattcctaa
     2761 acttggtgga caatcaatta taatataatc atagttgtcc ttgattggtt gaagtactct
     2821 tttaagcctg tgttcacttc ctacttgagt aaattccctc tctgcagaag aaagggctat
     2881 gtcggctggc aaaacatcaa ttcctttata gtccagaata gcatctgtaa tgtcagcctc
     2941 cccttttaat acttcataaa ttgtaggttg gtttgttcca tctatatcga aacctacatt
     3001 atctgtagca tttccctgag gatcaatgtc cactaaaagt acatttttac cgtaaacaaa
     3061 tttcaaacat gctgctaaat taacgctagt cgtagtttta gaaactccac ctttttgatt
     3121 ggctactgac aatattcttg ccattaaaat atcccccatt catcttatat tataatataa
     3181 ttatatcata atattgtgat attgcaatat cgtatatgca ttatat
//
LOCUS       DACDID010000195         3211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDID010000195 DACDID010000000
VERSION     DACDID010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.201"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            653..1564
                     /locus_tag="IV236_002515"
     CDS             653..1564
                     /locus_tag="IV236_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772673.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2051..2317
                     /locus_tag="IV236_002516"
     CDS             2051..2317
                     /locus_tag="IV236_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772674.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2389..2730
                     /locus_tag="IV236_002517"
     CDS             2389..2730
                     /locus_tag="IV236_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772675.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2785..3204
                     /gene="ssb"
                     /locus_tag="IV236_002518"
     CDS             2785..3204
                     /gene="ssb"
                     /locus_tag="IV236_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8772676.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
BASE COUNT         1158 a          534 c          704 g          815 t
ORIGIN      
        1 aggttatatg taataagtgg ggggagggct gtcggcgctt ggtatgaaag gcattctccg
       61 acttccaggt ttttgaccat tttctctcaa cgtggaaaaa ccgccactta tgagagaaaa
      121 tggtcaaaaa cctaagcggg aagtctccga ataacatacc aagcgccgac agccaacaga
      181 taaagaatac actactaaaa atagtagtag cataaaattt aaaagggaaa aaatcaaccc
      241 ctaccccagt cttggggtct caaaactaac tcccccctaa ccccctcttt cagcctcttt
      301 tgaaagaggg ggagaatcaa ccccacggcc aacgccccag tgttcttggg gctgtccaga
      361 acagaaactt gcgagagagt gggggtaaaa caagtgtaga ggaaacttga agacttgttc
      421 gaacagaaac ttgcgagaga gccggagtaa aacaagtgta gaggaaactt gaagacttgt
      481 tcgaacagaa acttgcgaga gagttggagt aaaacaagtg tagaggaaac ttgaagactt
      541 gttcgaacac aaacttacaa gagaggggag taaagcagtt gcagaggaat caggaagatt
      601 tgttcgaaca gaaacttgca agaaagcatt attaaattga aggtgatgaa atatgaagta
      661 tttattgcat ggctatttta ttgattattc tactcgcaat agtagtactt tagcgattct
      721 atgtttgctt catgaattga gagtggttac tagaaaacag ctatacaaac tattgaattt
      781 tgacagatca attagtaaac aaggagtaac gaatgcttta gtgagattat atgaaaaaca
      841 gttaatcgat cgaaaaaaat atggaacaga aatgtattat tatttgacga aagagggaca
      901 tcaaagcata ggtgggtatt atacgttgcc gaaagttcct gaatataatt tgaatcacca
      961 tttacaaatt aacaattatt tgattaaagg aatggcattg gcaaaagacc acccacattt
     1021 aaaacggatt atttcagaaa gacgtaccgt ctatgaaacg aaggatttaa atcctaagac
     1081 aaaaggaaga aaattttttg tgccagattt tatttttcgt tttcgagaca aagaaaatca
     1141 tgagatcaat tggtcctttg aaattgagct aacgttgaaa acacgtcgtc gctaccgtga
     1201 agcgattttt ccgaaatata ttcgtgagtt gcgaacacgt cctgaatcac atttaattta
     1261 tgtgacacct agtggcgcaa ttcaagaaga acttcatgcg tttaagcgta tttttttcaa
     1321 gcgcgaaact gaaaaagatg cgagtcaaca agaattgttt gagcggttgc atattttttc
     1381 agctttagag tttgaagagg gattaaaagc attattagag aatgatccag cagtcaattg
     1441 gcggacagta gaacgagaaa aaatttatcc gaatggcgct gttcaaagtc gtttgaattt
     1501 atttgaagaa tcaactgaaa atgaggaaaa accaaaagtg aagtttacgc ctgaagtatt
     1561 gtaaaagagg gtgttcgctt cgctcttttt ttgtgccagt ttgctggcac cgttcagacg
     1621 caagcgtctg aaccggctcg tttttaggaa ggactaactt gtttgattta tccgttgtca
     1681 aagaagtgga cgttcgagag tccctaatcg tatagcagtc attcggcgag tataacctaa
     1741 aacataacct gccaattgcc aagggtaaaa tgcacgctgc gcgcccttgg caatcgtcag
     1801 aacatgtttt cgagccggtt atgccgaacg accgctatac gaaaagggcc tctaaacaga
     1861 agtccacttt gatccttgac aactgaaacc caaaatcaaa caagcaggcc ttcgtctaaa
     1921 aacgagccga atgaagacct tgggggttgc acccccaaac ccccgtgccc ccgacacact
     1981 accgaaaagg tgggggtggg tggcgaaaaa atcaaataaa acattctatt caagaaagga
     2041 agaaacaacc atgacaacta aaaacgagca actgaaagaa caggcaagaa aagagttgca
     2101 agaaagaggt ttgattattg aggggagctt tgaaggagat tttgaaacct atattggttg
     2161 ttatgcaaga ccgataaaca aacctacagc cttagaccca atgaacgaac aagaagtaaa
     2221 agaacaagaa aaacatgcag tgaatggttt tccgcaagat ttcactgaat ggtatgagtg
     2281 ggaaattaac aacgggaagc ttgaaaactt cttataaaag tcagatgacg tgcaaagaga
     2341 atgattctct ttgtacggta aaaatataaa gaaagaagga aaaagaaaat ggcaaaaaac
     2401 acaccttatc aagtaggaga cattttttat agtagttggg gctacgaaca aacgaacgtg
     2461 actttttggc aaattgtgaa gttaacagaa aaaacagcat ggtttcagcc tattcaaaag
     2521 aaaacggtcg agcattattc gtccatgagt ggaaaagttg ttccaatgaa agacaagttt
     2581 atagagtatc ctttagcaaa aactaaagac agtattgtaa aaaaacgaat tgatccagaa
     2641 acctatcctg atatgttatg tggttatcgt agttgggaca cgtttcatag atataaaaat
     2701 caagctgttt atgaaagtag ttattattaa ggggatagtt tcccctttca aaaataataa
     2761 atagaagaat ggagagaaaa aatcatgatg aattcagtga atttagtagg acggttaaca
     2821 aaagaagtgg atttaaaata cacaccaaaa ggcaatgcga caggcacatt tattttagct
     2881 gttaatcgaa attatacgaa tgcaaacggc gagcgtgaag cagattatat ccgctgtgtc
     2941 atttggcgga aagctgcgga aactttagca aaatatactg gaaaaggaag tttgattggc
     3001 attaatggac gtcttcaaac gaggagctat cagaatcagc aaggacagac catgtatgtt
     3061 acggaagttc tagtgacaga tttctatttg ttagagagta aagaaatcaa tgagcaacgg
     3121 tcaaaatcaa tggcgaataa tcaaaataat tcttcaattc caggaaatga gattcaaatt
     3181 tcagatagtt atttgccctt ttaagtgaaa a
//
LOCUS       DACDID010000196         3187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDID010000196 DACDID010000000
VERSION     DACDID010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.202"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..242)
                     /locus_tag="IV236_002519"
     CDS             complement(12..242)
                     /locus_tag="IV236_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772677.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(336..674)
                     /locus_tag="IV236_002520"
     CDS             complement(336..674)
                     /locus_tag="IV236_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772678.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1602..1718)
                     /locus_tag="IV236_002521"
     CDS             complement(1602..1718)
                     /locus_tag="IV236_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8772679.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2194..3072)
                     /locus_tag="IV236_002522"
     CDS             complement(2194..3072)
                     /locus_tag="IV236_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772680.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1001 a          587 c          469 g         1130 t
ORIGIN      
        1 taagaactcc cttactgttg catactaaca taatccaaaa actcttctac ttcagctaaa
       61 gacattccac tttctttagt ccaaaaaaca gttttttcta tcgatccttc tctgtaaact
      121 gtaaatgtcg gagtcgagtt aattgagtag cgactcttga attctttcca ctcattttca
      181 tcttctctaa cttttgctac attcacgtaa taaatatttt cttttccctt ttctaccagc
      241 atatcgaata gcttcctgct tttgatgatc gatttcttgt gctacatcta gtaaattttc
      301 atcaatttct atatcataaa gtatttcact agtgtctaca tccacaatat atacttttta
      361 tcagtagcaa tttttgagta atccgttaaa atgatctcag tctcgtcact ttcatcagta
      421 taaatatacg ttaacaatga actcaccttt tcaatcggtg ttagctctaa gcctactgta
      481 ttttttacta ttgagtcatt atttaccacc ataacttcgt actgtatata ccatcctatt
      541 tggtttccat caatatcagt aattggctca ctagctaatg gagtctccgg aacaatattt
      601 tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt tatcatgttc ctcacttgaa
      661 actggttgag tcatagaaaa tgttacttgg ctaaacagag ggaaaataaa cactacaatc
      721 aacaaagtat aaaaaaaagc ccttcttttt cattttttat cttccaatca caaattgaaa
      781 aacttattcc tgcaactttg agtctagtat cacaatcgac tgactacaac tattcattta
      841 cattctactt taagttcctc cttaaccatt aattttatta aagatacttg taatagtgat
      901 tctcacaaaa tttcatgaaa aatactttat tactgtatct aaaattccaa tacacaactt
      961 atcgactatt atctgaaaac caaaacttat tattccttga tgttattcta gcaatagtaa
     1021 ctgctttttt atttttttgc atattttttc ggcgttactt tttaaagtta actttttcga
     1081 caaatagaaa gagatacgaa tcactgattt ttttgaagaa aagaaatgga gtgagctgaa
     1141 cgttagagat attgggagct ttcgtaactg cttattaaac gataatggtt atttgcaacc
     1201 gtattgctcg cttctacatg aaatatttca tgaaatattt cgcaaaacct ttgattatgt
     1261 agttacgttt ccattcttga ctaaaaatgt ttcaaaaacg aacaaaggtg ttaaaaggta
     1321 aagtgatcaa tactactggt caaaataaga ggttgaaagg gaattgaaaa ataactacag
     1381 ataatacttt tataaacgta cgtgctttgt ggttctgtag ttaactatat aactggaatt
     1441 agggaatggt atcaatttga aaagaaagat tctaccaatt tatcatatgt taaaaatgga
     1501 aaatcaacag cagttctttc ggaaacctta cacaaaaacc gattattgaa aatagccgaa
     1561 aaacgttttt cggctatttt tttaattaag tttccttctt cttacctttt cccactattc
     1621 cgtttcacat ctttgatgat cttcagcgca cgttttcgtg ttttgatatt tgggcttttc
     1681 aagcggcggt atgcacttga gatatctgtt ttttccatat ggacgaccca ctttcttctt
     1741 tgattgtagt catgctaaaa aacacaaatc gaaacaatta cgatttgtgt tttaaatggt
     1801 atcaaaagtt aaaatgaaag tcaacttgtt aaaacagtcg atcagactag ctgattgact
     1861 cctatctctg gaaataaatt ttcattatta cgttttagct acctaaaaaa ggccctcttg
     1921 tccgttaact ttaatatcta ttgttatagc tagtttacct attgaacgta ctgtgtgttc
     1981 gatagaacac taaaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa
     2041 ttcacgaaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt
     2101 ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtct
     2161 cacgctcttt taaactattt cgaagtacta aacttatgaa gtaagaatac ctaagccaga
     2221 gccaatgtgc tcgcctgcaa gcaattgctc ttttgcttgt cgctgtaaac tcgtactttc
     2281 tggataatca tgtatttggg catctactcg tccaaagatc caagggactc cactatcgtc
     2341 ctgttcatca actgataata atcgataatc ggctgcaaaa agacagattt ttgactcttt
     2401 tttcaaccat ttagtaagat tgggtgatag taaccaagta tctgtgtaca tcacttggta
     2461 atggtaatca ggaaaataaa gttcaaaaaa acgaatggca ctttcgtaac tatctcttat
     2521 cttattctga gataaatctg catcggaagg aatatgaaga gaaaggcact gctttccttc
     2581 taatcttaga tccgtgtacc ctttcggaaa atatgtcatt tcgtactcca attcaccaat
     2641 ccgaaagatc aagccactag tgtaatgcca aatccaaaaa ccacggtcaa agcgatagct
     2701 tcctgttact tggaaatgtt catgaataaa tctagtaaaa gcattcattg ttgccaaata
     2761 gatagttctt ggaatcttca atggttgata aatttttttc caactttgag aagctgcata
     2821 tagataaata gctaatgctg caaaaggttc ctcttcatag actttgtcta ttttctttgc
     2881 agtcgccttt gctttattag gatccattag ttcatgacta tattgctcaa acacctcatc
     2941 aaggatgttt tttgcttgta caatcatttg ctctataacg actttgggaa gagttaaagt
     3001 ttctgccaga atttggattt cattgcttat tttcttcatc aaatttcccc ttttcatatc
     3061 tttgaacctc attgtttaaa tgactgatta tccggacacc atatggtata aacatacttt
     3121 tattgtacac taactataaa acttaaactt tctatgaaga aagaatatct gtaagacttt
     3181 caaggaa
//
LOCUS       DACDID010000197         3165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDID010000197 DACDID010000000
VERSION     DACDID010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.203"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..605)
                     /locus_tag="IV236_002523"
     CDS             complement(60..605)
                     /locus_tag="IV236_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8772681.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(628..1113)
                     /locus_tag="IV236_002524"
     CDS             complement(628..1113)
                     /locus_tag="IV236_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8772682.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(1103..1915)
                     /locus_tag="IV236_002525"
     CDS             complement(1103..1915)
                     /locus_tag="IV236_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8772683.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(2054..>3165)
                     /locus_tag="IV236_002526"
     CDS             complement(2054..>3165)
                     /locus_tag="IV236_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8772684.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT          976 a          679 c          566 g          944 t
ORIGIN      
        1 aagttcatta gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt
       61 caataattcg ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga
      121 gcttgtccaa gggttttcaa acgaggtctg acaggtactt ggacatgttt gatatgcata
      181 ccgattgatg tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca
      241 ggatcattga actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga
      301 acaacagaga caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct
      361 cggttcaaat gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct
      421 tttaacgtac ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct
      481 ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc
      541 tcagccaata tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac
      601 ttcattttct aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag
      661 acttgcagtt ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg
      721 tatagaagcc actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat
      781 gatcataact aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct
      841 gaaagaacca gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg
      901 cggatatcct gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc
      961 cgttggacca taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc
     1021 attcgtagcc ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag
     1081 catagcatag atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc
     1141 cagaccagac acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa
     1201 caaatttttt actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg
     1261 ttattgcaga agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat
     1321 gagaaaatac ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca
     1381 tccttgtgcc gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga
     1441 gatggatcat ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg
     1501 ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat
     1561 acaattgatt gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg
     1621 ggatattttg ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca
     1681 ataacccaat tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt
     1741 taagcaattc caaagatata gattccagca taaatcgctc attaatatct agtgccccta
     1801 tagaagtcaa ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt
     1861 ggattcctcc accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat
     1921 tttcctccat gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat
     1981 atttttatct catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc
     2041 tttccttaaa tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca
     2101 ctttcattgt agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc
     2161 gaggaagttc atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta
     2221 aagcaataat cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa
     2281 aatctaacga atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca
     2341 aatccacttg ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc
     2401 cgctatccca ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag
     2461 tcaaagcccg tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat
     2521 tcccaactga atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc
     2581 ttgcatcact tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt
     2641 cactttggcc atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg
     2701 tcttcattgt ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa
     2761 aagcttctgg atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct
     2821 ctagtgcaat tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg
     2881 ctaaaggcat cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg
     2941 aatgcactgg agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg
     3001 ttttttgcca tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga
     3061 taccagttgt accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt
     3121 gagaaacaga caaagcaggt tgttccatta aataaagaat aggaa
//
LOCUS       DACDID010000198         3163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDID010000198 DACDID010000000
VERSION     DACDID010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.204"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..818)
                     /locus_tag="IV236_002527"
     CDS             complement(<1..818)
                     /locus_tag="IV236_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8772685.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
     gene            complement(832..2049)
                     /locus_tag="IV236_002528"
     CDS             complement(832..2049)
                     /locus_tag="IV236_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8772686.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2039..>3163)
                     /locus_tag="IV236_002529"
     CDS             complement(2039..>3163)
                     /locus_tag="IV236_002529"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8772687.1"
                     /translation="IKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          878 a          677 c          529 g         1079 t
ORIGIN      
        1 acttgataag gatgtaacac catgagggtt ttattgtctt gatcttcact gacaatcgta
       61 taatccgcaa tcaagcgatg ggcatctggg atatttagca cttgtttgca aaactcatat
      121 aagttttcta cctttttatt atctttcgtt cgccaactaa ataaaaactt aggatgcaaa
      181 tccttcggta aggcatttgc aaaataccga gtcgtttgtt cattggaaac cacaaacaac
      241 tgtaaggtcg aaaagatatt gttccgaaac atgccttctt cagcatattt tttgatttga
      301 ttaaacgcct gataaaaacc atcttttgca gttacttgtt ttagctcgat ttggataatc
      361 ggtaagccat tgatcaataa ggtgacgtca aaacgtcgat cccgcgcttc tatgttactt
      421 ccacgttttg caatttgatg gaccacttca taagtagaga ttcctccacc aatgtcttga
      481 ttggaataaa ggataagtga gacagaaccc aattgcggat tttcccgttc aatcgtgatc
      541 cgagccatcc cattttcacc ttttaaccat ttcgcagcat caaatggggt tttcgtccgt
      601 agcaataatt cggttttgat cgtttcaaac tctttatcgg tcaaaggaga atcattcagt
      661 tctgcttgat tatttgagat gattttttga cgtaaattgc tccaaagatc ttcttctgat
      721 tttagatccg gacgatacgt ccactgatta tgtccttccc cgaggacacg aatcaactgt
      781 tcttcgactt ccgcttcctc acggtgacta attttcattc ttcttccctc cctaaacaaa
      841 catcttttgc aagaagcctt ttttcgtttc cttcagcaaa tctaacttac gttgatgaag
      901 agcgatcgta tcatctagct gcttgaagaa ggcaccaatt ttttgttgtt catttatatc
      961 cggtggaaac tgaaatgtcc tttgttctat accaccttta gtcacatgta ccattgtcgt
     1021 tccgttagtt gattctttaa ttttctcttt atcagtcaat agccaagtat atagatactg
     1081 tttgtccaat tccttagata aaaatttaag tttccaaata tgataatggt agattacacg
     1141 ttcttcattc cacatttcag gaccgaagct tgttgcccat aaatataaga gatctccttt
     1201 attagcatac ttgtcttcct ctaattctaa gtttgaataa taccatctat cattagtatt
     1261 aaaattacct actcttaaaa ccctatattt tccagaatca agtaattctt gttgtttaaa
     1321 agctctacca ttcaaaaaaa ctaccacttc ccccaacttc cgctcttccc aatctcccgt
     1381 aaatcctgga aaacgaactt ctggcacttt tgctccgttt ttagggaaca ttttttgtaa
     1441 gaatcctttt ttcgtttcct tcagcaaatc taattcgtgc tggtgaagca tgatggtgtg
     1501 atcaagttgt ttgaaaaatg aaccgatctt gatctgttcg tcataagttg gaagtgagat
     1561 aggtgtcttt ttaccactat caattgtata ggttccgacg gttccaatgc ctgcaacgtc
     1621 acggatatac ttctgaattt tgttatttat gaacgagtag taaaagaagt ttccgtcaat
     1681 tttatgctca ttcttgaacc aaatgatgtt tccatcctta aagtaaattg ggttatcatc
     1741 agtgattaat aaggggacac ctattgatcc gacaccagtt acaagcaagt ctccttgaga
     1801 aactttacct gaatttttcg aatattcatt atatttttct tctgaaatgt aaagataatc
     1861 ggatggttcc tcattttttg cagctgagac aatatcacga gcacgtaaaa atctaatacc
     1921 tgagttagtc caatctgatt gatgaattcg cttcacagaa gtaacatcca tcatatctcc
     1981 taacttacgc tcttcccaat cttccgtaaa accaggaaac cggatttctg gtcgtgtatc
     2041 attgctcatg tttgaacacc tccagcgcac cttgtaacca cgcttcattt tcaggtgttg
     2101 tttgcaggga agagatagct tctaaaagtt ctttttctag cacttgctgt tcttgtcgta
     2161 tcttcttgat gtcatttccg acatgaacca tatcaattgg ttcttcttct tcgaaagtat
     2221 cgacatagcg tgggatattt aagttatagt cattttcttt gatttcatca aaggttgcca
     2281 aatgagcgta tttctcgaca tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt
     2341 tttcttccga taatttattc tgatttttcc ctttaacaaa ttcacgacta gcatcaatga
     2401 ataatacatc tcgagtttga cgattcttct tcaatacaat caccgtcgtt ggaatcgatg
     2461 taccaaagaa caagttcgca ggcataccga tcacagcata aatactgccg tcttctaata
     2521 atttttgacg aatgacccct tctgcagcac cacggaataa aaccccgtgt ggcaagacga
     2581 tagccatagt ccctgtttcc ttcaaatgat agaaaccatg aagaagaaac gcaaaatctg
     2641 ctttggattt tggcgctaat ttcccgtagc gattaaaccg agaatcatct aagaaggtcg
     2701 tatctgctga ccaattagcc gaataaggtg gattcatcac tactgcatca aatgtgtaag
     2761 gttcatctgt tggccagtcc ttattcaacg tatccccatt gcggagattc atttcttcgg
     2821 catctacccc atgtaaaatc aagttcattt ttgctagatt gtatgtcgtt gtatttagtt
     2881 cttgcccatg atatttaacg ttatctggat gagttaagta gttacgtaca tttaacatca
     2941 aggaacctga acccatggtt gggtcaaaga cactaaagaa tcgtcgttct ttttgatcca
     3001 acgtcacgat ttgtgccatc atatcagaaa ccatatgagg cgtgtaaaat tctccggctt
     3061 tcttccctgc ttctgaagca aattgactga tcagaaattc atacgcatcc ccgatcacat
     3121 ccccatcgtg acctaaaact tccacgtcat tcaatttctt gat
//
LOCUS       DACDID010000199         3162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDID010000199 DACDID010000000
VERSION     DACDID010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.205"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1125
                     /locus_tag="IV236_002530"
     CDS             <1..1125
                     /locus_tag="IV236_002530"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8772688.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1115..2332
                     /locus_tag="IV236_002531"
     CDS             1115..2332
                     /locus_tag="IV236_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8772689.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2345..>3162
                     /locus_tag="IV236_002532"
     CDS             2345..>3162
                     /locus_tag="IV236_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8772690.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT         1089 a          534 c          658 g          881 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa
      961 gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa
     1021 gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa
     1081 gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat
     1141 ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga
     1201 ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa
     1261 agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat
     1321 tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt
     1381 gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt
     1441 atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca
     1501 acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt
     1561 taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt
     1621 caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac
     1681 gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg
     1741 gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca
     1801 atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa
     1861 agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt
     1921 atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat
     1981 atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt
     2041 cttaatagat aacaatacca tggcttattt atttaataat acttgggata tctattttgg
     2101 taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc
     2161 ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca
     2221 aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt
     2281 agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag
     2341 aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact
     2401 tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg
     2461 ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt
     2521 gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt
     2581 tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga
     2641 agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg
     2701 aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg
     2761 ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct
     2821 gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc
     2881 agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa
     2941 tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt
     3001 atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca
     3061 agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga
     3121 tcaagacaat aaaaccctca tggtgttaca tccttatcaa gt
//
LOCUS       DACDID010000200         3158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDID010000200 DACDID010000000
VERSION     DACDID010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.206"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(482..1438)
                     /locus_tag="IV236_002533"
     CDS             complement(482..1438)
                     /locus_tag="IV236_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772691.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1547..1792)
                     /locus_tag="IV236_002534"
                     /pseudo
     CDS             complement(1547..1792)
                     /locus_tag="IV236_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1937..2359)
                     /locus_tag="IV236_002535"
     CDS             complement(1937..2359)
                     /locus_tag="IV236_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772692.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2498..2928)
                     /locus_tag="IV236_002536"
                     /pseudo
     CDS             complement(2498..2928)
                     /locus_tag="IV236_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          959 a          696 c          516 g          987 t
ORIGIN      
        1 tctataattt ctaagactga taaaaatatt ctatcggttc tttttgcaaa taaaaaaaca
       61 tatagaagct ccattagatt gaatcaccac aacacaatca aaggagcatt ccgtatgctt
      121 gattttcatt ctataaaact acttttagga atcaaagaaa atcacaacgg ttcaaactaa
      181 aagcaagaat atccgaagta ccattgtttc atcgatcatg agaaaagcat tcacctctgt
      241 ctgcagatgt tcttatatcg ctctgtcagt cctattaata aagtactgtt ttgttttcaa
      301 aaaaactatt acgagtactt tttaagagaa tgccttgcaa atattcatca cacaccgtta
      361 tataagtcaa atagtttctt tcagtcgtca agaaaagatt ctcctgtcct tggcattatc
      421 agttaagtag caacttaatt ctcggacttg ttctactcta gcaaaattta ttttcatcaa
      481 cttacgtcca aatgtttttt tgaataatta cttcatcgcg ggtctggaag acttgcctca
      541 ttcgataagt cactagcttt tggcgtcctt caggatttct agtatacacc aactgacatt
      601 tgggcagcga cgattgattt aattcgttta gaaaaaggtt agcgatttgc ttatttcggc
      661 caaagcgttc tggtaccgtc agaactatct ttttcccttc gattaaatac cgcggattct
      721 ccagcggtgc taaaagctcc ctcatagctt cagtaaaaag ctgtccttct cgatagccag
      781 cattctcaag atagcatcta aattcctgct gatcagccgc tacgattact tttctatcac
      841 taacttcacc aaggcgctgc aacgtcgttt gaacgcatat ggccaagctt tttttgcggg
      901 cacattcctt ttcccatagc ttttgtcgcc tacgaatttg gaaatattcc agcaagttac
      961 ctttaaatat agctgaccag aagcttagag gctctatatt ttttactact atcggcaatg
     1021 aagtttcttg tttttgcttt acctgatcga ctttcctaac gacgattggc atagataggt
     1081 gccagccatc agctaaagca gtactccatt gttctttcaa ccgacttcgt tcacaagaaa
     1141 aatagaacat cgactgagta taagtttcca tcgtggtggt cgtatattcc agtggtaaag
     1201 caaagcgatc gccaccactc tcaatttttg gtgaagattc aaaggtcaga cctgaaatat
     1261 tgcccaaacg ccgtagcatt cttttcaaat caggctgttc agacagagga actcccggta
     1321 atccaatcgc taaatgccaa atgttcgtta ctttattttg tgcacctagg cgtaagcccc
     1381 gccctcgtag ttgttgtgtt tgcacaaaag tggaagcttg attagccaag atcaacagat
     1441 taattgcagg acaatcccag ccttctccta gcaaactaac ggttcctacc aaaatattca
     1501 acttcttttc ggttaatagg cgagtaactg cttgcatcaa tgcttggcgg tttttgtcat
     1561 ccaccgtcaa ataaaaataa cccgacatat aaggatgttc attcataccc gtcgctagtt
     1621 ctgccagctc aacagttact agcattttcg gtaacagtaa gaactctcca cagatggcac
     1681 cgatttgctg agtgttacct aatagacgaa caacttcatc aaacaaagga accacgccat
     1741 aagctaaggt accatcactg cctgctaata cctccttgca aatacgatcc atcaaaatga
     1801 ccgctgtcag ttcttgcttc agctgaactt cttcttttaa caaaatttga caaactgctt
     1861 gctttttctc aggaatcgcc tgactaccaa cagtgtctgg atactctgga aacaaatgca
     1921 gcgactcatc aaacaatcat ccagcttttt tcaattgata aaaaataggc aattccggtt
     1981 gatcttcaaa catctgctga taaatagtga tcagctcttt tttacctaaa gatggaactg
     2041 aagctttttt accttgctgc ttagaactgc ctaatagctg ccaatgttcg ggtatcaatt
     2101 catagttatg atgattcaag taaattaatg cactagtgaa cagctcaaaa ttttcataaa
     2161 tttccttcga atgctcaagc ggatgctgaa caaattctaa agaaagcaaa taatccaccg
     2221 tctcttggct ttccattaac gcttccacca gctcattttg tttctctaaa aaggtaaaat
     2281 aatgctgagt agtttcctga ttagccggaa tcaaatatag aaaatcctga tgaggacata
     2341 gaacctcctc ctttaccaac tcgggaatgc taatttcttc gtcaatcagg ccattttaac
     2401 tcaatatatt gcttccactc cgtctgagag ctatcaatcg gaggcgtggc tgtcaaggaa
     2461 atcgtcaaca gattcggttg ttttttcttc aagtcaatca acaattcacc ccagcttttt
     2521 tttaaatgat gagcttcatc aaaaaactaa cagctccagc tgatcaccat aagttggctc
     2581 ctccttcagt cggttgtaaa tggtctgata cgtttccact gtaatatgac taggatgttc
     2641 aatcgcccca gaaagatagc aactatctgc tggatttact aagaaatatt cctcgattgt
     2701 tgttaaccac tgtttcttca atattaggga aggaaccaaa attaaggttt tacaatccaa
     2761 acgtcggatc atctcaattc ctaaaaaggt cttgccagcc cctggtggag ccactagatg
     2821 gactgaattg ttatttaaat aagtagcaaa gttagtcaat atacgcttct gatagctacg
     2881 ccactgaatt ttttcatcag atagccacat cccaggtccc cctctcacgt attttctcta
     2941 tcataatcta aatgaataca gcaaaaaaaa gatttcttca agaatatgtg tttttatagt
     3001 aatattttac ttactctaaa ctcaaatgaa aaggtaaaaa aactggtagt acaatctttt
     3061 aaattctcat tgttttgtac gttctttact atcaaaaaaa caaaaaaaac gcgtttctac
     3121 gcgtgaccaa aatttttagc tctataattt ctaagact
//
LOCUS       DACDID010000201         3141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDID010000201 DACDID010000000
VERSION     DACDID010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.207"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..489
                     /locus_tag="IV236_002537"
     CDS             13..489
                     /locus_tag="IV236_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772693.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(495..677)
                     /locus_tag="IV236_002538"
     CDS             complement(495..677)
                     /locus_tag="IV236_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772694.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(677..976)
                     /locus_tag="IV236_002539"
     CDS             complement(677..976)
                     /locus_tag="IV236_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772695.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(1081..1284)
                     /locus_tag="IV236_002540"
     CDS             complement(1081..1284)
                     /locus_tag="IV236_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772696.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1299..2045)
                     /locus_tag="IV236_002541"
     CDS             complement(1299..2045)
                     /locus_tag="IV236_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8772697.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2123..2431
                     /locus_tag="IV236_002542"
     CDS             2123..2431
                     /locus_tag="IV236_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772698.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2402..2659)
                     /locus_tag="IV236_002543"
     CDS             complement(2402..2659)
                     /locus_tag="IV236_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8772699.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
BASE COUNT          876 a          602 c          514 g         1149 t
ORIGIN      
        1 tgaggtgatt ctatgaggaa aaagctcaat tacattctta ctgcgacttt aaccatatat
       61 ataattctgt ttattacctt atctttcctg tcttcaatta aaaaaattcc ttttattaca
      121 ttgaataaaa atagtatttt gaaggagttc aataacactg actacatcgc atttctaaaa
      181 cttttttcag tctgtgtgct aatatcatgt gtaattattt ttattgttgc taaaacatgt
      241 tggacaatgt taaataaaaa ccaacgcgat gttgaaaata ttgctatatt tttaattaag
      301 attacttcct caatatcaat tatcattccg atgactgtaa ctttttcaaa agaacagttt
      361 aatgtagcgg ctacttatct ttcatttata gcgctatttt cttttgttct tcctaaaaga
      421 aataagagca actttgacaa caaggagctg gatgaagtac gcaatagcag cccaaaggaa
      481 caaaaataaa aaaatcatat tttagtcagt ccctcccgac tggctttttt cgctctgtgc
      541 tcagcttcat caagccccat aaaaatccaa accatgtaaa cgattgtccc tatcaacgct
      601 tgtctgcttc cccagaggcc taaagcgtag atgattagcg gtgcgctgaa tactagtgct
      661 ctgttgaatt tgcccattaa cttgcctcct tcaaaacatc tgaaggcgat aaacttttta
      721 cacgatattt gtttgcgtct ttccacttta aaaaccaaat aaaagtatgg taatgaataa
      781 atgtggttga gtgacctgga cgaagtattc cttctgcaaa ttcaggaatt gtttccattt
      841 ccttgcagta ttcaactaaa gtagtttttg acatgccgtg gaacttctcc aagattaaac
      901 tttgtcggta ccagtcgtct ggttttactg ccttctcagc agcttctatc agctctgaaa
      961 gtgttggttt tttcacttat tatccctcct aaaaattatc gttccatact cgatgtttaa
     1021 catttcttga agctctgatt ttgtaagaat gattttatct tccaacgact ttacctccct
     1081 ctacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt
     1141 tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat
     1201 ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc tatctcgttg
     1261 agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc
     1321 atttgagggt aatagccctc tgcgattaat aagttgtaaa taaacactcg ccctttctgt
     1381 gtccatttgg tattcattac aactttagtg ccgccatcgg ctttcggtat ctcacttgtg
     1441 tgcgattttg tgtatccttg gttcatatgt tttcggtaaa gtatccattg tccgctaact
     1501 ttatgctgaa cacctaactc gtttagcaat ttgtttagag caattgccga cattccgtaa
     1561 tcagctgcaa tttgagaagt agctaccgta tctgtcgatg aaagaatcgt atctaagtag
     1621 ctaatctttg gttcgtactc ggcaatttgt tgttcgagta attggttctt ttcttctaaa
     1681 tccgctgcta aacgcaatgc ttgtgcaaaa ctttgaggta cgtttgaata gctacctgtt
     1741 tttctaattg ttggaaggac ttcactcgtt acccaacgtt taaatttttt ggcagaggga
     1801 agttttgatt ttaagattaa actgtaaagg cctgactcgt taatcaaaac aactgctgtt
     1861 ccattgacgg tgaacgaatc gttctgcgtc ttatcctcca agtccacatg atctcgaata
     1921 gctttttgcg gattcgaata tcctagaacg tctgctacat cttttcctac aaaatatggt
     1981 tcatcattta ctaaaatagt tcgaacttcg ttttgctcga aattaaaaat ttgtggtgta
     2041 ttcatatttc tcattccttt ctttggtata atttccttat cagtgtgaca ggctgaaata
     2101 attgttaagg aggtgaaaat ttatgaaacc agaatttgta attactatgc ttgatggaga
     2161 aaaaatcaaa atatttgccg atactttggt cgttggatac gatgatgcgt caaatatcga
     2221 agaaaaagat ggaatgttct attcttctca aatttacgtg aataccttgc aaggtgatat
     2281 tggggtatct actcttgcaa catcaagacc gcttgtaggt cttatcggct ttttgtcaaa
     2341 agcaacattc ttttctctcg gcgatgatat agattcggat gaaactgtca tctataaaac
     2401 atcagctgtt aaaagtatcg gtttcaaata gtaattcttg ctcgttgata gcggaagcca
     2461 acttttcttc caacacatcg gcgagctttc tttttgttgg attcatttca aagttgatag
     2521 tcttgagaag catttttgca tccttaactt tttgtccttg caattccttg atgattttat
     2581 cggcttggct atcaattcca tgtgttagat catctatcat tttttgtacc ttgattgttt
     2641 ggctcatttc cttgttcatt gtttagcctc ctttaataaa tgattttttg tattttttaa
     2701 tttccataaa ttcctcaaat tgctctttgg tattattacc acgaccatac atattgtgaa
     2761 attctttatg gcaactgaca caaagtgtaa cgccgtttga aatcgctgcg gatttagttg
     2821 ttgcatggct aaatcacgaa tcacaagtat ctactaaagg cgaatcaatt tcgccaaaag
     2881 aaattgttca agcttattta gatattcatt atgctgttat ctatggtcag cttccagaag
     2941 accgtaaaaa cgatgactaa acgtgagcta aaatctctgc tatggctgca accatggcag
     3001 agtcttcttt atctaccgcg tgttccatcg ccacttttgc ttgtttcagt atgtctaatt
     3061 tcaaatcttc gatttttgcc gagactgttt tttccatatt cttctctcct ttcttttata
     3121 ttcgtaaaca aatttaacaa c
//
LOCUS       DACDID010000202         3124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDID010000202 DACDID010000000
VERSION     DACDID010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.208"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..414
                     /locus_tag="IV236_002544"
     CDS             106..414
                     /locus_tag="IV236_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8772700.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="IV236_002545"
     CDS             441..740
                     /locus_tag="IV236_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8772701.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..1465
                     /locus_tag="IV236_002546"
     CDS             914..1465
                     /locus_tag="IV236_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8772702.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1611..1904
                     /locus_tag="IV236_002547"
     CDS             1611..1904
                     /locus_tag="IV236_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772703.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1897..2193
                     /locus_tag="IV236_002548"
     CDS             1897..2193
                     /locus_tag="IV236_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772704.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2264..>3124
                     /locus_tag="IV236_002549"
     CDS             2264..>3124
                     /locus_tag="IV236_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8772705.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDE"
BASE COUNT         1173 a          439 c          625 g          887 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat tggcagatag
     1441 tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat aagtagtttt
     1501 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1561 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1621 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     1681 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     1741 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     1801 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     1861 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     1921 ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc gaatatagat
     1981 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2041 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2101 gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg ttgtcatgct
     2161 ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat taattgactg
     2221 ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa aatggttgtt
     2281 attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata cgaggactaa
     2341 agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc aatcaactga
     2401 acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat
     2461 tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat ctactcaact
     2521 agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa gctacaatcc
     2581 tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg atggaaattg
     2641 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2701 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2761 gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2821 tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac agttaaaaca
     2881 aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag aagaatctac
     2941 tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt caagttggga
     3001 agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt ctcctgataa
     3061 aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3121 tgaa
//
LOCUS       DACDID010000203         3096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDID010000203 DACDID010000000
VERSION     DACDID010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.209"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(301..663)
                     /locus_tag="IV236_002550"
     CDS             complement(301..663)
                     /locus_tag="IV236_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8772706.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            886..1638
                     /locus_tag="IV236_002551"
     CDS             886..1638
                     /locus_tag="IV236_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8772707.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1778..2701)
                     /locus_tag="IV236_002552"
     CDS             complement(1778..2701)
                     /locus_tag="IV236_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8772708.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2716..2988)
                     /locus_tag="IV236_002553"
     CDS             complement(2716..2988)
                     /locus_tag="IV236_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772709.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1008 a          590 c          512 g          986 t
ORIGIN      
        1 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
       61 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
      121 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
      181 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
      241 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
      301 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
      361 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
      421 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
      481 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
      541 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
      601 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
      661 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
      721 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
      781 tatcacttaa tagtataaca taatttcatg agaaaaaaat acaatttttt cttgaccatc
      841 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
      901 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
      961 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
     1021 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
     1081 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
     1141 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
     1201 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
     1261 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
     1321 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
     1381 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
     1441 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttatc ttacagctgg
     1501 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
     1561 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
     1621 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
     1681 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
     1741 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
     1801 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
     1861 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
     1921 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
     1981 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
     2041 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
     2101 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
     2161 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
     2221 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
     2281 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
     2341 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
     2401 tctgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
     2461 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
     2521 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
     2581 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
     2641 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
     2701 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
     2761 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
     2821 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
     2881 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
     2941 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
     3001 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
     3061 aagaaaagtt ttattcctct ttcattatta ccaaag
//
LOCUS       DACDID010000204         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDID010000204 DACDID010000000
VERSION     DACDID010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.210"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1243
                     /locus_tag="IV236_002554"
     CDS             <1..1243
                     /locus_tag="IV236_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772710.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="IV236_002555"
     CDS             1256..1888
                     /locus_tag="IV236_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8772711.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="IV236_002556"
     CDS             2093..2320
                     /locus_tag="IV236_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8772712.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="IV236_002557"
     CDS             2389..2655
                     /locus_tag="IV236_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8772713.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="IV236_002558"
     CDS             2645..2914
                     /locus_tag="IV236_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8772714.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DACDID010000205         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDID010000205 DACDID010000000
VERSION     DACDID010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.211"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IV236_002559"
     CDS             177..359
                     /locus_tag="IV236_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8772715.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IV236_002560"
     CDS             462..746
                     /locus_tag="IV236_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772716.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IV236_002561"
     CDS             762..998
                     /locus_tag="IV236_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772717.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IV236_002562"
     CDS             1020..1274
                     /locus_tag="IV236_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772718.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IV236_002563"
     CDS             1356..1541
                     /locus_tag="IV236_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8772719.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IV236_002564"
     CDS             1608..2003
                     /locus_tag="IV236_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8772720.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IV236_002565"
     CDS             1993..2370
                     /locus_tag="IV236_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772721.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IV236_002566"
     CDS             2371..2766
                     /locus_tag="IV236_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772722.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DACDID010000206         3002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDID010000206 DACDID010000000
VERSION     DACDID010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.212"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IV236_002567"
     CDS             complement(65..1285)
                     /locus_tag="IV236_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8772723.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IV236_002568"
     CDS             complement(1305..1910)
                     /locus_tag="IV236_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8772724.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IV236_002569"
     CDS             complement(1959..2891)
                     /locus_tag="IV236_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8772725.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          824 a          628 c          522 g         1028 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tt
//
LOCUS       DACDID010000207         2889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDID010000207 DACDID010000000
VERSION     DACDID010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.213"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IV236_002570"
     CDS             367..555
                     /locus_tag="IV236_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772726.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IV236_002571"
     CDS             545..805
                     /locus_tag="IV236_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772727.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IV236_002572"
     CDS             846..1073
                     /locus_tag="IV236_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772728.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IV236_002573"
     CDS             1138..1710
                     /locus_tag="IV236_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772729.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IV236_002574"
     CDS             1711..2022
                     /locus_tag="IV236_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8772730.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IV236_002575"
     CDS             2013..2273
                     /locus_tag="IV236_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772731.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IV236_002576"
     CDS             2434..2793
                     /locus_tag="IV236_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772732.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
BASE COUNT         1013 a          437 c          626 g          813 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttatt
//
LOCUS       DACDID010000208         2852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDID010000208 DACDID010000000
VERSION     DACDID010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.214"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..134
                     /locus_tag="IV236_002577"
     CDS             <1..134
                     /locus_tag="IV236_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8772733.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="IV236_002578"
     CDS             complement(220..1314)
                     /locus_tag="IV236_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8772734.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="IV236_002579"
     CDS             1503..2264
                     /locus_tag="IV236_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8772735.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IV236_002580"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IV236_002580"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8772736.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          913 a          567 c          481 g          891 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tt
//
LOCUS       DACDID010000209         2747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDID010000209 DACDID010000000
VERSION     DACDID010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.215"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(133..744)
                     /locus_tag="IV236_002581"
     CDS             complement(133..744)
                     /locus_tag="IV236_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772737.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(857..1114)
                     /locus_tag="IV236_002582"
     CDS             complement(857..1114)
                     /locus_tag="IV236_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772738.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(1711..1989)
                     /locus_tag="IV236_002583"
     CDS             complement(1711..1989)
                     /locus_tag="IV236_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772739.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(2023..2274)
                     /locus_tag="IV236_002584"
     CDS             complement(2023..2274)
                     /locus_tag="IV236_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772740.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT          849 a          551 c          368 g          979 t
ORIGIN      
        1 cctttaatat caaaatatct actggtcgta ataatattcg ctatgaattc ctactactcg
       61 tataagaaag gccaaatcat cagcttttac ttgctgatga tttggcttgt ttctatttat
      121 cgtaattgtt ttctaatcac caaaattgtt aacaatcggt tcagatattt cccagttgat
      181 taaggtaatt ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa gtacagttct
      241 tatgtatcca ctttctccta atataaagtc tacctctctt ttaaaacctt cctcgagatt
      301 tctaagggct atatgtggtt ttaatgacat tgattgtaca ttttccccca tataatattg
      361 cgcacttcct cgactaaaat agattcctag atcgtcccca aaaggtagat cttctctacc
      421 actagctaat ctacttctcc atcttcgttc tcttttcata gaacctgcgc tgaactcaca
      481 cccgttaaac tcagctatga cagtatcttc aattatgtta atctgtccat ctagttcgta
      541 ttcaatccta aatggaaatt ctccataggt gatttccggt tcgggtattt taggtgagat
      601 taatgcaaag aaaggaaata ttaagcttgg caacatcaaa aaaactcctc ctatgatcac
      661 aattcccgat gtaatccaaa atataatttg tacaattctc attgatccat tgtattccat
      721 tttattcccc ctattactat tcatatccgc ctatctatat cgatcttcca atgcagagat
      781 aattgcttcc ataccttgat tttttactga gttacctatg cgagatatca ttctccacta
      841 aggtgcatca tattgtctat ctaacatgat taattctcca ataggtgtat actcctccaa
      901 aaacaacatt taatgcatct ccctcgacaa cataagctac catgtttgct gtatatgtgt
      961 ttgagtctaa ttcataagca gataaattta ctgtcgctgg attaaatata atcgcattcg
     1021 tatttatagc tactgaattt gctgcttcag caccaccttt tgaatgtccg atcattgtga
     1081 tttcaatagt aggatttacg tggacagccc tcaccgttta ccctgaccat tatagaaatc
     1141 gcttactttt ttgaactacg caacaaagga tacaatgctc ctaacttcac tgtctccatt
     1201 tttgtccact aaggaatata aaaaataggc attaattcat ttaattcggt cacaacacgt
     1261 taccttttaa atcattatac tgtatctcat acattattat caataaacta ttataaaaat
     1321 gcaaaaaatg ctttatcaat ctattttgta attggtcata atatcattga aatcataccc
     1381 tgtatcgatt tttcctatga atctcgtcta tttgtgattg acgaaaaaga ggcaagacag
     1441 acactggaat ggtaatttta cccactattt tgctccaact atttgtaatc gtacccactt
     1501 ttgaaatacc gagatgatca ttttaattga tatgctgatt cccaagcaaa accccaagaa
     1561 attaaatgct ctatcgcatc tagcttcttg ggttttgctt atataaattc caacatttaa
     1621 attttttatg ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa tatcaagatg
     1681 tcaaattatt cgtgttgccg atgactttga ttaatttttt tcaaattgtt tattaagact
     1741 aatgctgtct ttttgcagat accaatgttc gaacttttta acattatcat atgatcctgc
     1801 aagttttaac ttcttaccat tagatgttat caaagttata attaaccttg cctttttccc
     1861 attaggatct tttattttat gaattttctg caggtaatct ctcctaatgg cagctatttt
     1921 tacctcatca gtgctgataa accattgatc atccacatac atactgcctg ttatgttgtg
     1981 tttttccatt tcttccgaga acgaaaagtt taaggctcgt tcttacttcc aaagttgaat
     2041 gagtccatgg attggactta gtgtgagaat tgagaacaac aataagaatc catatcctcc
     2101 cgcaaacatc cctaaggcta tactgtctct ccatccataa gtaggattag aagagaaaag
     2161 agtcaccgtt tgtaataata tgaaaaggaa taatccagca caaacaccaa agataacaac
     2221 tccaaacatt gaatttttat acaatgacca tttatttata ggcttttctt tcattttact
     2281 tccccctact gttctgttca cacggaatga attcaaagtt cataagccct cctctttgaa
     2341 catatgaata cgacttgcta actatacttt aaaaaagctt gttttaagcg gttataggcg
     2401 agttgtaatc tctattcatc tcattagcaa ataacttgcc gaaaagtcta ctgaaattaa
     2461 agcagatagt ggaagaatta tttagccagg atagacacta tttgtgaaac acatgtcttt
     2521 acttttgaaa ttctatgatg aaaagccttg gaatacagta gctcttaatt ggtaatactt
     2581 tttccattat aaataacctc ttttcatttt atatttatcc cctctaaaaa taatatctaa
     2641 tctaatattt taaacttata tttccctcgc tgtgctaata gagcattact aaataaatag
     2701 aaactagaaa aaagcattac gcctgccaga ataaaccaat tatttgt
//
LOCUS       DACDID010000210         2735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDID010000210 DACDID010000000
VERSION     DACDID010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.216"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IV236_002585"
     CDS             174..518
                     /locus_tag="IV236_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8772741.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IV236_002586"
     CDS             519..938
                     /locus_tag="IV236_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8772742.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IV236_002587"
     CDS             complement(1030..1380)
                     /locus_tag="IV236_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772743.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IV236_002588"
     CDS             complement(1346..2566)
                     /locus_tag="IV236_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8772744.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1014 a          493 c          528 g          700 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caact
//
LOCUS       DACDID010000211         2621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDID010000211 DACDID010000000
VERSION     DACDID010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.217"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..555
                     /locus_tag="IV236_002589"
     CDS             <1..555
                     /locus_tag="IV236_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8772745.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRN
                     ISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQLIEN"
     gene            689..>2621
                     /locus_tag="IV236_002590"
     CDS             689..>2621
                     /locus_tag="IV236_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772746.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYIL"
BASE COUNT          947 a          393 c          460 g          821 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg tggtactttt
      241 gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctaagccaa
      541 ctgattgaga attagaaaca atctgcagta gggaagaatc atttacacaa aattattgac
      601 gctcccttat aatgagaact tttactaaca taatttatga taaacttact atataatcat
      661 attaattaaa tagttaggag atctgcttat gtttatatct actcttcaaa ttcgaaattt
      721 tagaaattat gaatccgaaa tctttcgatt cgataatgag acaaatacta ttataggtga
      781 gaatgattca gggaaaacca atgctcttac tgcattgaga atcttattag atgatagtta
      841 ttactatagt agtaaaacac taaaagaatc agattttttt catggaattc aaaatgggtg
      901 gcaagggcat tggattatta tctctgcgac atttgaagga atcagcgaag aagaatttga
      961 caatgagatt tgtgcttcca tttctttaaa ttctgaaagt caaacaatct tagaagaatt
     1021 gatttcaaat gcagataaag gtgtaggtag tatttctcta tttattagac ctaacaaagc
     1081 tattagaaag caattatttg atatttccac tgaattagac tctcatcaat ttaacgaatt
     1141 tagaaattct attcgtctat ccgactatga attttactac acatctaaat caaatctaaa
     1201 tttctgtatc aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca atatagctag
     1261 taatcctgaa gaagacgatg aagctctgtt gggctcaaga attgacatgt cagatatttt
     1321 caagcatatt tcggttgttt atgttgacgc tcttcgagat gttcttagag aaatgaaaaa
     1381 caatcgaaac ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat catcagataa
     1441 tgttgagtca ttaaaaacaa ttatacagca attaaatgag actattacca gtgttcctga
     1501 aattaggaaa attggcgaaa atattaatcg gcaactaaat agcataattg gttcagtgta
     1561 ttctcctaat cttcttcttt cctcaactat gtcagatgat atgggttctt tagcaaaatt
     1621 tctttcaatg aagccagaac aaaatatcga cttagactta ctagggttag gacatctaaa
     1681 tatgatttat ttagctttga aaatagtcga atttgaagcg tgtcgttcaa gagaactact
     1741 taatattatg ttgattgaag aaccagaagc tcatattcat catcatattc aaaaaactct
     1801 gttcgaagga ttaaatcttc aaaaaaacta tactcagatt ctcatgacca cacattctgt
     1861 acatttagct gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt ttaatggcaa
     1921 atctatcgtt atgaatccag caaataatct gaactcattt gctaaagaaa agttaaaaaa
     1981 gaacaatcta aatctaataa acgcagtaga gagatattta gatgttaaaa gaaatggatt
     2041 gcttttttct aaaggtgtta tcttagttga aggagatgct gaagaaatac ttattcctca
     2101 aatagtaaaa aaattatttg gtataaactt agacgaactt ggaataggtt tgattaatgt
     2161 tggtagtact tcgtttgaat atatcgctag cttgtttgac gatgatagaa tacagagaaa
     2221 gtgtgctatt gtaactgatt tagatgaaca ggcaatccca tcagaacatc gattgtataa
     2281 aagcactgca caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc tttttaatga
     2341 aaacaattgg gttaattgtt tttatgctaa tacaacattt gaaattgagt ttttagatgt
     2401 gaataaagaa aatattctaa cttatgtcag tccaattata gatgacgcat tcaaagataa
     2461 aaaaacaata actgcttata aagaagaaat acaaagtgaa caatatttag aaagaaattt
     2521 agcgatgtta aaactagcca atcatgttgg taaggggtgg tttgcaattg agttaggtga
     2581 aaaaattgac tttttaatca aaattcctaa gtatattctt a
//
LOCUS       DACDID010000212         2609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDID010000212 DACDID010000000
VERSION     DACDID010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.218"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..316
                     /locus_tag="IV236_002591"
     CDS             83..316
                     /locus_tag="IV236_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8772747.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            313..720
                     /locus_tag="IV236_002592"
     CDS             313..720
                     /locus_tag="IV236_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8772748.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1857..2375)
                     /locus_tag="IV236_002593"
     CDS             complement(1857..2375)
                     /locus_tag="IV236_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8772749.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          873 a          449 c          393 g          894 t
ORIGIN      
        1 ttagacatga gagggactat gcttgattta aaatcaacaa gcgtatatac tgtatataca
       61 aagaaaaagg agggagaaga ctatggcaat taaagaaaga aaatttaggg aagtggggaa
      121 ctcagtagta ttaactattt caaaggagtt tcttaaagaa aatgggttaa atcccggaga
      181 tacggttttt ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac aagccttatc
      241 tgatgaaatt gatttagcaa tatctcaagc attaactgaa tatgatacta cttttagaga
      301 gcttgtcgat aaatgagtat tcattaccta gaagccaaag atattataaa aatgaatgta
      361 atccaaataa aaaaatactc tcctaatgaa ccccttggtg taaaggatgc gaatgcttta
      421 gaaatgtgtg tagggcaact tcgagcctca gcttttggtg aagaggttta tccttcgatt
      481 tatgaaaaag cagctatcct acttatacaa ttgataaaga aacatccttt tcacaatgca
      541 aataaacgta ctgctttctt agctactttt gtctttttga aaatcaatgg acagctttta
      601 gctattgatc aaaaagatgc tgtaaattta gcggtatata ttgctacgta tgataaggat
      661 ttcgatcaac taaaatatga agtgatcaac actataaaga cacatacgca gcccatttaa
      721 ggagttactg tatgtgtctt tatagggtta ttagctgcta tagcgaaata attgaagtaa
      781 attacaaata gctataattc aaatccaact atatagaaaa cgtaaatttt ctatatagtt
      841 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
      901 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
      961 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     1021 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     1081 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     1141 accttttaca tcttgcagaa atcggtactg ggattaaact aagcaataaa aactaatgtt
     1201 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     1261 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     1321 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     1381 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     1441 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     1501 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     1561 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     1621 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     1681 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     1741 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     1801 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     1861 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     1921 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     1981 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     2041 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     2101 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     2161 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     2221 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     2281 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     2341 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     2401 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     2461 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     2521 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     2581 tagtaaaatc ggactggttt gcatatcat
//
LOCUS       DACDID010000213         2595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDID010000213 DACDID010000000
VERSION     DACDID010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.219"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(2..1426)
                     /locus_tag="IV236_002594"
     CDS             complement(2..1426)
                     /locus_tag="IV236_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8772750.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(1690..>2595)
                     /gene="guaC"
                     /locus_tag="IV236_002595"
     CDS             complement(1690..>2595)
                     /gene="guaC"
                     /locus_tag="IV236_002595"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8772751.1"
                     /translation="DTTVTLGKHTFKMPVVPANMQTIIDETIAEFLAENGYFYIMHRF
                     DEAARIPFIKKMKKRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGHANSV
                     IDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGT
                     GGWQLAALRWCAKAARKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESPGET
                     KVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQHKGSLKDTLVEMQQDLQSSISYAG
                     GRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          712 a          575 c          421 g          887 t
ORIGIN      
        1 attattctgt taaccacttt tctagaattt tcatctttga ataaaacaac ggtatttggt
       61 tttcatccag cctactgtaa ttgattctga ttccatttat atcttgccca aaaagtagac
      121 ttggtgcaat tccaagaccc atatctacta ctttttctgc tagttcgggg tctagttttt
      181 tcccatccca agtaatccag gcatagtaac cacctaaaat tgaatgaagc ttgaaaaacg
      241 attgtgaatc tccccaagcg cttatcagtc ggcttttttc tttcaacttt tttcttacat
      301 cttgcatttt caaatcaatt gcttcattaa atacattagc cactgtgatt tgtgtaagga
      361 tattcatcga aaactccatc atttcttgcg cttgagctag ttgcgagata aaagtctcag
      421 gtcctatcac ccaaccgatt ttcgttgttt ttcctaaaac cctagataac gaaccgacgt
      481 ataacacatt atccggatca atcgttcgta taggaaggac ttcttcttgg tttagaaatg
      541 aaagatccgt aaatacatcg tcttcaataa ttggaagccg atactgtcga cacaattcta
      601 ctagcttttc tcgtcgtttt tgactcatca cttttcctgt agggttttga aaattcgggt
      661 tgataagcac tgctttcaat ttatttttat ggatcgattc tttcagtttt tctaagtcga
      721 tcccttcttc atccatcggt atcccaaata aacgcgttcc tgaagctttg aaaagaggta
      781 atcggtaaaa aaaagaaggt gactctacag ctatcccctc tccagctgaa agaatcgttt
      841 gtaaaatcaa aaagattgct tgctgaccgc caccagtcac aagaagctgg ttgggggaaa
      901 atgccaattc aaatttactt tccaaatacc gtttgatttt gttacgtaac ggagtatagc
      961 ctaaaagatc catttctttt tcttccgcta agatttcttc aagagaataa gaaggaaact
     1021 caaaattcgg caaaagctca tctggcatct cgctagcgta taaatcaaga aacgatgttt
     1081 tttggatctg ctttgcttct cttatttttt gctcatacca gtccgtctgc tcataacgag
     1141 gagagatcat ttgccgccaa tcgattcttg gaacagcgaa tttcccccat tcagtctgat
     1201 taacaaacca tccgctgcca cgtttgctcg ttagtacccc aagctcccta agctcttcca
     1261 atgcatggac aacggtggaa cgattcacac caaaaagttg ccctagtttt cgttctgatt
     1321 caagttgact tccaggattg atccttccgt tttcgatttc tctcataatc tgttccataa
     1381 tttgacgata aatcggtcct tgttttttct ctactttttc ccacataagc ttctcttttc
     1441 tattgtctat tttttctttt gattttaccg aatttacgag aaaaaagaaa aacttttttt
     1501 gcaaaacata agggactgtg acaaaaatct tatcacagtc ccttattctg aataatgatg
     1561 ttcaaaagct tgtatctgcg ataataattc cagccaaaca aaacatataa agaactattt
     1621 ttgtttggct gttcttatta cttaatgcag gacaactttt tcacaacatc ctaatatttt
     1681 atttattttt tagattgtgt ctccattaaa aatcgaattt ttcacgatga catagtcaac
     1741 ttttcgaatg gcttccagat ctcttcctcc agcataagaa atagatgatt gaaggtcttg
     1801 ttgcatttcc actagtgtat ctttcaaaga tcctttgtgt tggatccaga ttttctttcc
     1861 ttcaacattt cgtttttcgc ctttttggaa ttctgaagca gaaccaaagt attctttata
     1921 cacgattcca ttttcgactt ttgtttcgcc aggagattcc tcgtgaccgg caaacaatga
     1981 accaatcatg accattgtcg caccaaaacg gacagatttt gcaatatcgc catgtgttct
     2041 gatcccaccg tcagcgatga tcggcttgcg agctgctttc gcacaccatc taagagctgc
     2101 cagttgccag cctccagtcc caaagcctgt cttgattttt gtaatacata cttttcctgg
     2161 accaatcccg acttttgttg catctgcgcc cgcattttcc aattcacgga cggcttccgg
     2221 tgtaccaaca ttaccggcaa tcacaaaagt ttcaggcaaa tattttttaa tatgctgaat
     2281 catatcaata actgagttag catgtccatg cgcgatgtcg atagtgatat aatcaggatt
     2341 tagcgattct tctgccaact tctctataaa ggaatattcc tctttcttga cgccaacact
     2401 gatcgaggta atcaatccac gctttttcat ttttttgata aacggaattc gagctgcttc
     2461 atcaaaccga tgcatgatat agaaataacc attttccgct aagaattctg cgattgtttc
     2521 atcaataatc gtctgcatgt tggcaggcac tactggcatt ttgaatgtat gtttacctaa
     2581 tgtgaccgtt gtatc
//
LOCUS       DACDID010000214         2560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDID010000214 DACDID010000000
VERSION     DACDID010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.220"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..1014
                     /locus_tag="IV236_002596"
     CDS             7..1014
                     /locus_tag="IV236_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8772752.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1026..2030
                     /gene="neuB"
                     /locus_tag="IV236_002597"
     CDS             1026..2030
                     /gene="neuB"
                     /locus_tag="IV236_002597"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8772753.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2027..>2560
                     /locus_tag="IV236_002598"
     CDS             2027..>2560
                     /locus_tag="IV236_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8772754.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          935 a          332 c          512 g          781 t
ORIGIN      
        1 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
       61 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
      121 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
      181 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
      241 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
      301 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
      361 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
      421 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
      481 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
      541 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
      601 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
      661 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
      721 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
      781 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
      841 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
      901 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
      961 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     1021 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     1081 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     1141 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     1201 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     1261 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     1321 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     1381 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     1441 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     1501 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
     1561 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
     1621 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
     1681 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
     1741 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
     1801 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
     1861 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
     1921 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
     1981 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
     2041 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
     2101 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
     2161 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
     2221 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
     2281 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
     2341 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
     2401 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
     2461 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
     2521 ttggagagtt tttcccgtcg gagctcctag aatagatact
//
LOCUS       DACDID010000215         2544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDID010000215 DACDID010000000
VERSION     DACDID010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.221"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1747
                     /locus_tag="IV236_002599"
     CDS             <1..1747
                     /locus_tag="IV236_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8772755.1"
                     /translation="RKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSRDKNYL
                     SSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVG
                     VEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSP
                     DKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVY
                     SGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLGFNGLV
                     VTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERL
                     RDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAITLVKDK
                     QDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISP
                     EEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWPASKGT
                     PDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKG
                     ISPIDAYCGLKDTFI"
     gene            1775..>2544
                     /locus_tag="IV236_002600"
     CDS             1775..>2544
                     /locus_tag="IV236_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8772756.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSM"
BASE COUNT          937 a          321 c          504 g          782 t
ORIGIN      
        1 acgaaaaaaa ccatatcatt tagatgaaga tgctatttct tggattgaag agacgattga
       61 tgctatgact ttagaagaaa aaataggtca attatttgtt aatatgggat ctagtcgtga
      121 taaaaattac ttaagtagta tggtcaatca gtaccatatt ggagctgtac gatataatcc
      181 aggaactgct gatgaggtat atgatcaaaa ctatatccta caaacagaaa gcaaaattcc
      241 tttattaatt gctgctaata cagaagcagg tggtaacgga gcttgtacag atggtaccga
      301 agttggtgta gaagtaaaaa ttggtgcaac aaatgatgcg aaatatgctt atgaattagg
      361 gagagtttct ggaattgaag catcggctat tggatgtaac tggagttttg ctcctattgt
      421 agatattaat agaaattgga gaaatcctat catttctagt cgatcttttg gacaaagccc
      481 tgataaagtt ttagaactta gtttagctta tatgaaaggt attcaagaga gtggaatttt
      541 gcctgcagct aaacatttcc ctggtgacgg tattgacgaa cgagatcaac atctatcttt
      601 tagtattaat agtttaagta cagaagaatg ggataattct tttggaaaag tatactcagg
      661 cttaatagaa gctggattac cttcaattat ggcaggtcat atccatatgc cagcttattc
      721 aaaacatttt aatcttgatc tgaaagatga agatttactt cctgcaactt tatcaaaaga
      781 attaatcact gacttactta gaggaaaact cgggtttaat ggattagtag ttactgatgc
      841 tagtcatatg ttaggaatga ctggggcaat gaaacggtca gaattattgc ccacttctat
      901 tgcggctgga tgtgatctat tcctattttt taatgatcca gatgaagact ttaattatat
      961 gatggaaggt tattacaaag gaattatttc agaagagcgt ttacgtgatg cattaagaag
     1021 aattttaggc ttaaaggcta aactaggttt gcataaaaaa gaaaagaata aaattttgtt
     1081 accgaaacaa gaagctatgg aaaaaattgg tttaattgaa aataaatcaa tttttaaaga
     1141 ggtagctgat caagcaatta cactagttaa agataaacaa gatatttggc ctattgtacc
     1201 agaaaaatat aaaaggattt tactggtaga tgtacatggg gtaaaaggtg ggtttggtga
     1261 agtagttgct ggaaatagac ctcaggccat tgatttattg aaacagattt tgtcagcaaa
     1321 aggatttgaa gttgaaaaat acatttcacc tgaagaagaa ttgaaaggat tatcgcaaga
     1381 agaaaaaatt aaaaatatag ctggaatata tgctgctaag agaccaatca aagatttaac
     1441 tgaaaaatat gacttgatta ttaatatagc taatgttaac ccaggtacag ttcaaagaat
     1501 tgtttggcca gcaagtaaag gtactccgga tattccattt tatattcatg aaattccaac
     1561 aatttttgta tctgtacagt gtccgtttca tttagctgat gtgcctcaag taaaaactta
     1621 tatcaatact tatgatggaa aacaagaaac tttagaaata ttagtggaca aactaatggg
     1681 cctttctgaa tttaagggaa taagtccaat agatgcttat tgtgggttaa aggatacatt
     1741 tatttaatat ttaaattata taggagtgat ttacatgtta tatcccatta tgacagagac
     1801 gaggcaagta attgatttaa gcggtctttg gagatttaag ttagatacaa atgatgactt
     1861 acaagaagaa cttgctttag aaccaatggt tgaaaaagag agttatccaa tttcagttcc
     1921 ttcttcttat aatgatttat ttgaaacaga gaacattaga aatcatgttg gatgggtgtg
     1981 gtatgaaaaa gaagtgattc ttcctaaaaa attattagaa gaaagattag ttcttcgttt
     2041 tggatctgca acacatgaag caaaagtatt tttgaatgga aaattgatta atcaacacaa
     2101 aggagggttt acgccatttg aatcagaaat taatcagttt ataaaagatg gaagtaacag
     2161 aattacagtg gctgtaaaca atgttattga cgatagcact ttacctgtag ggattttgaa
     2221 agaaacgata caggaagatg gagacataaa aaaagaaaat ttggttaatt ttgacttttt
     2281 caattatgca ggcattaata gacccgttaa attgtataca actccgaaag actatataaa
     2341 ggatgtcact attaatacaa aaataattga ttcagatgca aatgtagaat ttaatatcga
     2401 tacagaatgg accggagaaa ttctaatagc aatctatgac gaagaagctc aacttatagc
     2461 agaagctaag gggaaagaag gagtgattca acttaagaac ccaacacttt gggagcctct
     2521 aaatgcttac ttatatagta tgaa
//
LOCUS       DACDID010000216         2509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDID010000216 DACDID010000000
VERSION     DACDID010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.222"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..1518
                     /locus_tag="IV236_002601"
     CDS             193..1518
                     /locus_tag="IV236_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8772757.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1511..1861
                     /locus_tag="IV236_002602"
     CDS             1511..1861
                     /locus_tag="IV236_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772758.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1858..2037
                     /locus_tag="IV236_002603"
     CDS             1858..2037
                     /locus_tag="IV236_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772759.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2173..2463
                     /locus_tag="IV236_002604"
     CDS             2173..2463
                     /locus_tag="IV236_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8772760.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          890 a          379 c          497 g          743 t
ORIGIN      
        1 actcttttag agtgagagtt tttattagat tttcaaatta attagtaaag tgatagaata
       61 aattgatcat acaaatcata agaagaagca gggtgatagc atacctatgc ttcttttttt
      121 atgtatttag ctatttcttc tttacaacat cgaacatatg tttgtataat gtggaagaat
      181 ggagtgttat ttatgatgga ttattcaaaa gagccagtaa atgattactt tttaatcgat
      241 atgaaaagct tttacgcaag tgtcgaatgt atagaaagaa atttagatcc attaacaaca
      301 gaacttgttg ttatgagtcg atctgataat actggttcag gattgatatt agcttcttct
      361 cctgaagcaa aaaagcggta tggtattaca aatgtgagta gaccacgtga cttaccacaa
      421 ccatttccta aaacactaca cgttgttcca ccacgtatga acctatatat caagcgaaat
      481 atgcaggtaa ataatatttt cagaagatat gtggctgatg aagatctact gatttactcg
      541 atcgatgaat cgattttaaa agtgacaaaa tcactgaatc tttttacgac tgaagaaaca
      601 cgaagtcagc gaaggaagaa gctcgctcaa atgatccaag aacgtattaa agaagagtta
      661 ggtttgattg ctacggtagg cgtcggcgat aatccccttt tggctaaatt agccttagac
      721 aatgaagcaa agcataatga aggatttatt gcggaatgga cgtacgaaaa cgttcctgag
      781 aaagtatgga atattcccga aatgacggac ttttggggaa ttggttctcg aatgaaaaaa
      841 aggctcaacc aaatgggaat tttgagcatt agagaccttg ctaactggaa tccttatacg
      901 attaagaata gattaggtgt gattggatta cagctgtact tccatgctaa cggaatcgat
      961 cgaacagata tagcaatccc gccagaaccg acaaaggaaa aatcttatgg taattcacaa
     1021 gtgttaccac gagattatac tagaagaaat gaaatcgagc tggtggtaaa agaaatggcg
     1081 gaacaagtag ccattcgtat caggcagcat aattgtaaaa caggatgtgt tcatttaaac
     1141 attggtacgt cgatcttaga aacgagacca ggattttcac accagatgaa aatacctatc
     1201 acagataata caaaggaatt gcagaattat tgtcttttct tgtttgataa gtattatgaa
     1261 ggacaagaag taaggcacgt tggaataacc tatagcaagc ttgtgtacac cgattcgctt
     1321 cagctggatt tatttagtga tccacaaaag caaattaacg aagaaaattt agataaaatt
     1381 attgacaaga ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttcaatgtta
     1441 gaaagcgcta gaagcattac tagaagcaca cttgttggtg gtcatgcggg cggaaatggg
     1501 ggaattaaaa atgattgatt acaatgatcg tggacgttta aagtgggcag ggttttattt
     1561 atctgatcat actgaagaaa ttgatgccga caatgaacaa cgaaccagtc aaaatttagc
     1621 aaaagaacaa atgacgactg aagagattgc ggaagtattg aacgctgcag tcataaaaaa
     1681 tagatcagta tctattcaaa aagaagaacg aaacgctgaa ggatactatc cagaagatat
     1741 tgttggcaag atcttaggta gtgatgagct ggggatctac gttggaaaag acaaaattca
     1801 ctacgacgaa atacgtcata tcgaatttta ccaggataat aaatggagtc gattagaatg
     1861 aacattatcc agcaatatga gctaaagtat ataacctttg atcagctatc agaagagatt
     1921 tggggttatg gccaacgttt gataaatgag gttggtgttg agcgcttctc tttctatgta
     1981 gaagctgcag caggttatca taattttagg ttttatattt tcccgttata tatataaggt
     2041 attttacgga ttctatagag ttatctctat gaatcctttt ttgtttttac aaataggagt
     2101 agatactaga ataatgcgtg tgttaaacta ttattgcaaa cgcaataata aaagaaagta
     2161 ggcttacgca ccatgtctat caatacattt gtttactcgc acccgatcaa tgtttacatt
     2221 atcaagaatt taggaatcac tgtagaacaa ttttgtgagt tatatgccta cccacaagga
     2281 acggttgcaa gctggataac ccgacaacgt agaataaaat cattacctgc ttcattcgtt
     2341 tatgatttaa gcctggcctc tagtttaaac atgtctgatg tttatgaaaa gctcctgtct
     2401 ttagaaaaag aatatgattc cttcaagatt aaacatgata aaaaaataaa aaaacatata
     2461 taatatcaat cacaattgta taatattacg atatcttaat atcatgata
//
LOCUS       DACDID010000217         2485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDID010000217 DACDID010000000
VERSION     DACDID010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.223"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            280..486
                     /locus_tag="IV236_002605"
     CDS             280..486
                     /locus_tag="IV236_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8772761.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            486..737
                     /locus_tag="IV236_002606"
     CDS             486..737
                     /locus_tag="IV236_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772762.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            752..1189
                     /locus_tag="IV236_002607"
     CDS             752..1189
                     /locus_tag="IV236_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772763.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1182..1889
                     /locus_tag="IV236_002608"
     CDS             1182..1889
                     /locus_tag="IV236_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772764.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2270..2449
                     /locus_tag="IV236_002609"
     CDS             2270..2449
                     /locus_tag="IV236_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772765.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          904 a          412 c          520 g          649 t
ORIGIN      
        1 tgcacgttca ctaagatatg gtgtcttgca aagcaagaca aaatagaaaa aacttcctct
       61 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
      121 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt
      181 ccttgtcgct tttcgttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
      241 aggaaataaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
      301 tggggtgttc ccttcagcga attattccca aactatcatg caccacatac tgtcgaatgc
      361 gattgtggag aaagagcgaa atgtatcaaa agctatcgtt tgtatcaatg tccaacatgc
      421 gggaaaaaat ataccttgtc ttatggcgat tatgttttga ttgatgagaa agtaaagaaa
      481 cggtgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
      541 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
      601 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
      661 caggatatga actgcattat caagaaaaca tggaaactta tgaaatcgaa tattggttaa
      721 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcaa aattactatg acgaatttgt
      781 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
      841 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
      901 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa
      961 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc
     1021 aagcactatc aaaccttccg aatatcaagc cttagaactg acttatgcaa aattcgcaca
     1081 gattaagaaa gctaaagcaa tggctagcga ctggctagat acttttgaaa atatcgaaaa
     1141 aaatggtgca ttttatacga ttgaatcgga ggagaaagat aatgaataag gtggtttatc
     1201 agctaacaac agaagatgtt tcgcaatttt taacaaacaa agaacacttg aatgaagtaa
     1261 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
     1321 tggaagaaca gttgtatatg gaaagtgaaa cgagtcaaac gaaggttaca gtcagccata
     1381 atcgtttaga cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg gagcttctac
     1441 gactagaaac agaaaaatat atcatgaatg tttacctaga aaggagtgag ctggtcgaaa
     1501 actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa
     1561 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaagatgcga
     1621 cggtagacga ttattacctt gtatttacta gatttttgac gtgtgggacg gaatctatcg
     1681 tgttaatggg aatggattat gaactcactg tacccgaagc atttttgagg gaatatgtag
     1741 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagacacct
     1801 acacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
     1861 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag gatgtgtgaa
     1921 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgatccaa
     1981 ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaaa gcccgataac ctaagagaaa
     2041 gaaggagatc catatgaata aaatcattcc tctattgttc gtactgtttg ttggcgagat
     2101 acttatgcgt catggtgcag ctctacattt aaacttcgta gtattcttag gaatactggt
     2161 attttgtttg atcttttggc gctttgtgtt aacccttgtg agtctaatat tcaagttgat
     2221 tgcagccata tttttaatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
     2281 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagca
     2341 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     2401 gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac gtcacttttt
     2461 atcctaaaaa ctaaaagtga tagca
//
LOCUS       DACDID010000218         2481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDID010000218 DACDID010000000
VERSION     DACDID010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.224"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IV236_002610"
     CDS             complement(<1..1097)
                     /locus_tag="IV236_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8772766.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>2481)
                     /locus_tag="IV236_002611"
     CDS             complement(1227..>2481)
                     /locus_tag="IV236_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8772767.1"
                     /translation="RASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQT
                     DSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFV
                     SEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVE
                     VEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKN
                     LDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSL
                     LLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGN
                     NILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLV
                     LLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          750 a          481 c          365 g          885 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct g
//
LOCUS       DACDID010000219         2426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDID010000219 DACDID010000000
VERSION     DACDID010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.225"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1478)
                     /locus_tag="IV236_002612"
     CDS             complement(<1..1478)
                     /locus_tag="IV236_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772768.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPL"
     gene            complement(1576..1851)
                     /locus_tag="IV236_002613"
     CDS             complement(1576..1851)
                     /locus_tag="IV236_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772769.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            2083..>2426
                     /locus_tag="IV236_002614"
     CDS             2083..>2426
                     /locus_tag="IV236_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772770.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLR"
BASE COUNT          798 a          443 c          315 g          870 t
ORIGIN      
        1 agtggaagga tatcttcatt caatttttga atcacagaag ttgcattaaa aacgtctaat
       61 cgatagacat tgcttctctt atgattaaag tttcctaacg ctttctcttc ctttaaccta
      121 tttaggtatt cgagcatctt caaaggaaca tcttcatcat ctaatttttt tatcaatcct
      181 ataaaagtca taagtgatag taacttatta catttatcta tgtcataagt tttaccatag
      241 taagaactca tttttttagc taattgttcc cccgacaaaa tacaagtcac actttcctta
      301 ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg catttcctct
      361 agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt gtcaaaaagt
      421 acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag gttctctaat
      481 agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac aactccaata
      541 aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg cccagtttta
      601 ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct taagttttta
      661 cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat ctcattaaaa
      721 gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc ttctttgtcg
      781 tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc tctccctgaa
      841 tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc agtaatatct
      901 aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc aaaaaacatt
      961 ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa gtattcgtaa
     1021 acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac aatcttgtat
     1081 ttttctagat tttcagagta ccgaatactt ttttgtatcg caaacgcaga attatgaact
     1141 aaattgatag ataaaacctt ttctacgctt aagtatctac tatcatcttt actcaaatct
     1201 actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat tactctggtt
     1261 tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat aaatgattgt
     1321 gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt atctaaacac
     1381 ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa tttttcttga
     1441 tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc cttataaaat
     1501 tctgttaaag actaaaacat acagataata aaaacaaact ctttattact atatgtttta
     1561 gtcttatttg tttactcaac ctttataccg ttccatgaaa ctatcaatat cagatttttt
     1621 aaatctaatg accgtatcaa tttttataac aggtaacccg tattgagaaa catattttct
     1681 aatcgttcct actgatacac ctagatattc acttgcctgc tgatagtcca tatactctag
     1741 agtgactcca gaaataatgt cttctagcat aggctttagc actgtaatta tctctttcac
     1801 ttgttgttca acttgtatag aaacaatgct atcaatgatt tgtagagaca tatttccccc
     1861 tcctttcgtg attgcttgaa caaattttct aagaattatc ttcgtatttt ttgctcaaaa
     1921 taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca
     1981 tacacataat acctcgataa aaaaggggtg tcaacagctt tgtaaaattt atttgcaaca
     2041 cccctttttt gaggtatact aattttagga ggtgtggtca atatgataaa acgtccacca
     2101 ataaattatt tagaacgaaa aaagatactc ggaacaaaaa taaaagctat aagaaagagt
     2161 aaaaaattaa cacaaccagc atttggtctt atgattaaca acggtcaatt aattgacaaa
     2221 aaaactatct acgaatggga gaagggaacc tatttaccaa taccagagcg attatcccga
     2281 attgctgatt tgggaaatat gagtatagag gagttagttt gtggcaatgt agaagaatat
     2341 attttgggaa ttattcttta tagagatagc attgtactag atggtataac atttccagat
     2401 aaaaacctgt tccagcatct tcgtca
//
LOCUS       DACDID010000220         2424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDID010000220 DACDID010000000
VERSION     DACDID010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.226"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(326..991)
                     /locus_tag="IV236_002615"
     CDS             complement(326..991)
                     /locus_tag="IV236_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8772771.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1117..>2424
                     /locus_tag="IV236_002616"
     CDS             1117..>2424
                     /locus_tag="IV236_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8772772.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEK"
BASE COUNT          751 a          387 c          435 g          851 t
ORIGIN      
        1 gaaatataaa aaagaactaa aattgatcta aattatgtgc agcttgacta cgtaccaatc
       61 tatattactt actagattgg tcgttaatct attcggatcg aatgagcaaa atatttttgt
      121 gaatgtccat ccttgattat gattccaaaa agaatcattg ggaggctgtg gcgtaaatat
      181 tatactataa gccagaaata cctatcattc tgaacttttt tatttaggaa ctttcatctc
      241 aaggcttttt caattaattt gtacccctgt tttttggata agatcatgtg cattgatttt
      301 ttctatctct acgttcatct cctacttaat gcctatctgg ctcctgaggg cttcttttta
      361 aggcgtcgtc atagtcttgg ctgaggattt cttggtaatc tgagggaatg gcttcccaag
      421 tgagcaggcg aacgttcatg acgtgttttc ctttgtgttt gattacagca tggtttttct
      481 ctttttctac ctctccagcg tgaaaagaca gccatgtacc atggccaaag atatcattac
      541 atacccaacg ttcgatggaa ttgtccacgt atcctacagg gagtgtggta tagccgattt
      601 gtggtttcaa aaaaggattg aatcgatcga tcgtttcatt tttgtattga taattgataa
      661 gaaaaagata gaccaaacaa aagacgagca gaaagaaaga tagtgtcttt ttgagtgaga
      721 agggttgttt tgatgaataa gatttcttgt taacaagagg ttcatctggc aatgttggtt
      781 ctattgattc ggtagctgat tcaatagaag gaaaatcaat cggcagacct aaagtatgat
      841 gtaaaagtga cagcatttca aggtctggca ttgtacgaga attttcccaa ctagaaatcg
      901 ttttatcgga aacaccaatt ttttcgccta attctttttg cgtaagctta caatttctgc
      961 ggtaagttcg tattttttct ccggtgttca tcttcttctc ctttcgattc tacagtatat
     1021 agatttattc tacatgacct atacacttga tagatacgtt tttttgatga aaaacggtat
     1081 gattttatca aagaatcatt taagtaaggg agagtggtga taatcaaaaa aaagttcaca
     1141 gttttactca caatcatagt gaccggtatc atgagtggtt ttgccttggt tagtataaat
     1201 aaacaggtac tctatcggac aaatgaattt ttccatcaaa cagatactta tactccgatt
     1261 cgattgccac aacattttat tgggcaagaa gaatatgagc agctaatcat ggtatttgaa
     1321 aatgtctcat cagaaactga gctttcttat atgaaaaaaa cagtaaatga atccaatgaa
     1381 gtagatacat cttggaactt caagatacag ccaaagcttg aaatgacatt gtattatcaa
     1441 ggggatgtca aatcttctca cgagaaggta ccctattcta atacctcctt tcgcgtaacg
     1501 tccattgaag aatcgatcaa aataaaagat tttgaaggtg aatttttctt gatgacgaga
     1561 gaaaaagaag cctatgaacg gttcatttca ctattcactg atcgattcaa tcaagcattt
     1621 cacactgatt atgccactaa tatttttgaa gactttgaaa atagctattc gtattcggtt
     1681 atttcaaata cagacgtata taattatcga gtgtatttaa ctattggatt gattctttta
     1741 ttccttttgt tatcatcctt tttcttattg atgaataaag aaatcagttt attgttgaat
     1801 aatgactttt cattacatca agtattgtgg tgtttattgg ggaaaaagaa aatcattatt
     1861 acattcataa ttgcaggaat cattggtggt tttattttta tgtattataa ggagcaattt
     1921 ctttatttgt tccagatgta cgttttcttg ttcagcggaa tatatggtat ctctttgatt
     1981 tctcttttat tagttgaaaa aatcgcttcg ttaagagaag cgttcaacta tatccaatac
     2041 attcttttta catttggatt gttgttttct cttcttttga ccgttgccaa tgtcgaatta
     2101 gcaactatcg ttttttcttc ttcacagtta gctgtttccc atgaaccacc aaaagaaatc
     2161 gctggtgagg actatcatgt attttatcca gtaaccatcg ggaaaaacca cgtggatttt
     2221 atttattcca atcgttttgc atcagcagca gatcaggaat tatatgaaac tttgaataaa
     2281 aacggaagta tattagtcaa tgtatcagat tacttgaatg aagaaaatga acagtttggc
     2341 agagggataa aaataaatct gaattatctt aagaaatttc ctttgattga tgtctctgga
     2401 aggttgatcc aaataaccga aaaa
//
LOCUS       DACDID010000221         2419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDID010000221 DACDID010000000
VERSION     DACDID010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.227"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            513..1739
                     /locus_tag="IV236_002617"
     CDS             513..1739
                     /locus_tag="IV236_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8772773.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1847..2317)
                     /locus_tag="IV236_002618"
     CDS             complement(1847..2317)
                     /locus_tag="IV236_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8772774.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          898 a          359 c          479 g          683 t
ORIGIN      
        1 ttactttaaa agaaaatcgt tttactttga agaggagcat atctatccat taaaagatga
       61 tggtaatgca atcactagtt cgagtcattt taagagccat gcagtgagct ggaaataaaa
      121 cgaatagaag atgggtttaa caaaaggacc agtcatagga agaaattgtc gtgaaatcga
      181 atgataatct gaacggtagg caaagactgt taaaaagatg ggaaaggtat gtgcgatcat
      241 tttactgcac gattatttgg aaaatacctt gttgttcgct ttttctggaa aaaatacata
      301 aaaaaagata aaacgattgc gaactgcgtg tattcggtct ttcgatcttt ttcacggtat
      361 ggtatgatag ttaagaattt tgagaaattt taaaaaataa taatcgttac acaaaaatgt
      421 tttttaaagt tttcttatga ttaaaaagtg aatttgctga ttgaacagtt atatgagaca
      481 atattttaga aaatgtgaaa cagaggtgta ttatgaagcg atggcaaaaa gttctattaa
      541 ccttgttagg ggtattcgtt ttgctgattg ccggattatc agcatacgga atcagattta
      601 tgggagaagc gagccaaaca gtaaacaaaa tttctaaaaa ctctaatcga atatcctcca
      661 aacgttctga gagagtaagc attgatgaca aagaaccttt ttcggttttg ctattaggtt
      721 tagataccgg tggattagga agaacggaac aaggacgttc tgatacgatg atggtagtaa
      781 cagttaatcc gaaacaaaaa aaatcaacga tcatcagttt ggaccgagat atttatacga
      841 atatcgtagg ttatggaacg gtagataaat taaatcatgc ctacgcattt ggtggtgtcg
      901 aaatggccat ggattcaatt gagcaactgc tcgatattcc tattgatcat tatgtaacga
      961 tcaatttgga tggcatggaa gatttgatca atgcggtcgg tggcatcaaa gtgaataata
     1021 aaattgactt tactttggat ggtgtccatg taccagtagg tgaacaaaca ttgaatggag
     1081 aaaaaggttt agcctattct cgaatgcgac acgaagatcc agaaggcgat atcggtcgac
     1141 aacgtcgtca gcgggaagta gtgacgaaaa tcgtcaataa agttttaagt ttagatggcg
     1201 taagcaatta tagaaaaatt ttaaaagcag ttgagaacaa tgtgacgacg gacttagatt
     1261 gggatgacat gcttgatgta gcgacgaact acaccccggc gtttgaaaca atcaaacaag
     1321 accagttgca aggcgaagga caaatgatca atgatatcta ctatcaaatt ttaggaaaaa
     1381 atgatcttct ttcgatccaa aatgaactga agaaacaatt aaatataaaa acaagcgata
     1441 cattacctaa tttgaaaaat gagaatgctt caatgatgtt ctttgatgat tcggaagatg
     1501 gcgattcgac aaacggtgat ggatcgaata gtgaagggta caccgattat tcttcctatt
     1561 cagaaaacac atatgatcag taccaagatc cgaataccta ttatcagcaa acagaagata
     1621 caagtcagta ctatacacca agtcaagata ctcaaagtta tgatccttac gctggacagg
     1681 aacaaaatgg ttatggttat gaccagaata atgagtatgg aacaacaagt gggtactaga
     1741 aaaaaatagt ttggttaata aagagtgcca cctaaattag acaaaaaatc taacttaggt
     1801 ggcacttttt ctatgtgtta aattacagga cgtctctttt tagcgcttag tttaaaaaat
     1861 aaattgaata attcaataga gttgcgaata gtacccataa ataataagga atcatgagcc
     1921 acattgtcag gcgatgatgc agccacaact gatacataag cactgacaag aaaataacta
     1981 aaagtgtaat atcgattgct gcaatgaaaa tatttcttaa attaaaaaat agagcagacc
     2041 atataaaatt aaaaatgaac tgaatcacaa aaagaccgac aagattgttt cgctgttttt
     2101 tgttgttgtg attaagtaac aaataaagag aaatacccat caatatatac agtaaggtcc
     2161 acatcggccc aaatatccag ctaggtggtg caaatggtgg aagttgcaaa gaatagtaat
     2221 attttccggg atttccgcta agcaaccctg ataaaaagcc taatatgaca attcctataa
     2281 cacaaaccca aaaacgaata tcttttatca tcttcatttt actaactcct ttctataata
     2341 ttagtttacc atgaaggctc acagcaagac ctgtatttag tttgtgaagt aagaaaaaaa
     2401 ttgataattg ttgttatta
//
LOCUS       DACDID010000222         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDID010000222 DACDID010000000
VERSION     DACDID010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.228"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV236_002619"
     CDS             5..1150
                     /locus_tag="IV236_002619"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8772775.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV236_002620"
     CDS             1321..1779
                     /locus_tag="IV236_002620"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8772776.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IV236_002621"
     CDS             1938..>2407
                     /locus_tag="IV236_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8772777.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDID010000223         2405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDID010000223 DACDID010000000
VERSION     DACDID010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.229"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(362..865)
                     /locus_tag="IV236_002622"
     CDS             complement(362..865)
                     /locus_tag="IV236_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8772778.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1224..2249
                     /locus_tag="IV236_002623"
     CDS             1224..2249
                     /locus_tag="IV236_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8772779.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          772 a          373 c          453 g          807 t
ORIGIN      
        1 ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat tacttataaa
       61 acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga cggaattcct
      121 ttctagattg agatattgac taggaagaat gttaagattt taagccttta agctaaaaaa
      181 aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct
      241 gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa tgactgaata
      301 aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt tacgaacgat
      361 tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga aataagaata
      421 aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga tgtttgttta
      481 gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc aaatcagtga
      541 tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag cttacaagga
      601 aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc
      661 caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc gtattttcaa
      721 aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg agaaattgtg
      781 gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa taaatcccta
      841 ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta taccatcgaa
      901 aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa
      961 aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag atagcgagtt
     1021 gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg ttttttgttc
     1081 taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa gaaatagttg
     1141 ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag ctttttttcc
     1201 aattactagg aaggagggat agattgttca agacttattt tcgtttcaat aaaatacaag
     1261 ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta tggttaggtt
     1321 acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt acttttttct
     1381 attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc ggttgcggac
     1441 tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt gaaccatttg
     1501 ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat atttctaaac
     1561 caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag ctgccaatcg
     1621 ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg
     1681 cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa gcaactttaa
     1741 aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac agcgctgcct
     1801 tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat tggttgacct
     1861 tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat cgacaagtcc
     1921 aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt
     1981 ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca caatttctag
     2041 ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg atgctatcta
     2101 cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta gggatcgtca
     2161 taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg caacattatt
     2221 taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa aagaggttat
     2281 gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt ctcagcatta
     2341 agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata tttattctat
     2401 caaaa
//
LOCUS       DACDID010000224         2390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDID010000224 DACDID010000000
VERSION     DACDID010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.230"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            197..2059
                     /locus_tag="IV236_002624"
     CDS             197..2059
                     /locus_tag="IV236_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772780.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="IV236_002625"
     CDS             2059..>2390
                     /locus_tag="IV236_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8772781.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDID010000225         2334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDID010000225 DACDID010000000
VERSION     DACDID010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.231"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..>2334
                     /gene="esp"
                     /locus_tag="IV236_002626"
     CDS             8..>2334
                     /gene="esp"
                     /locus_tag="IV236_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8772782.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          848 a          370 c          476 g          640 t
ORIGIN      
        1 ttgaataatg gttagcaaga ataataagag agtatttcta gaaaaaacaa agaaacgagt
       61 attgaagtac agtatcaaaa aattaagtgt tggggttgca tctgttttga ttggtgttgg
      121 tcttgtcttg ggaacaactg aactagttaa agcacaagat gaaatttctc caagcagccc
      181 acttgaaaca gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt
      241 tcaggagaat cctggttata cgaaaaatta taacttttcg gatttacaat ttagtcctca
      301 agaactaact ggagacacac taaaaggcaa tacaattgga tttgaagttt acggaaaaca
      361 taatattgca gcatcaacta aaaactggga aatccgtctt caattagatg aacgattagc
      421 taaatatgta gaaaaaattc aggtcgatcc aaagaaagga attggatctt ctagacggac
      481 ctttgtaaga attaatgatt cgctgggtag acctacaaat atttggaagg tcaattatat
      541 tcgtgcaagt gatggattat ttgctggggc agaaacaacc gatacgcaaa ccgctcctaa
      601 tggggtaatt acgtttgaaa agagcctaga tgaaattttt aaagagatag gcatagacaa
      661 tctcaagacc gatcgtttaa tgtatcgaat ctacttggta agtcatcaag acgatgataa
      721 aattgtaccg ggtattgata gtacgggcta ctttttaaca gattcagatg atttctataa
      781 tagcttagat gtatctgaaa ataatcctga ccagtttaaa catggatcag taagtgcaaa
      841 atatgagaaa cctaatactc aaacaaaaga tggttcagga tccacaggtg ctaatggagc
      901 tataatctta gatcataagt taacgaaaaa ttataatttc tcatattcat cttccgcaaa
      961 gggcacgcct tggtatgcta attacaaaat tgatgaacga ttagttccat atgtagcggg
     1021 tatacagatg cacatggttc aggctgacaa agtaacatat gatgtttcgt ttgaatccgg
     1081 aacaaaagta gctgatttag caatcgagcg acgggaaggt catgaaaatt atggcatagg
     1141 ttcaataact aataatgatt taactaaact tatcgatttt gctaatgcaa gtccacgtcc
     1201 agtcgttatt cgatatgttt tacaattgac taagccatta gatgaaatct tagaagatat
     1261 gaaagcaaca gcacaagttg aagaaaataa accatttggt gaagatttca tctttgattc
     1321 ttggttgtcg gatacgaata aaaaattaat ccagaacact tatggaacag gttattatta
     1381 tttgcaagat attgatggtg atggaaaccc tgacgataaa gaagagagcg gagacacgaa
     1441 tccatatatc gggaaacctg aattagaaga agtatatgat gttgacacaa cagttaaggg
     1501 gaaagtattc atccacgagt tagcgggaac aggtcacaaa gcccaacttg ttgataaaga
     1561 aggtactgta ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg ctccaatttc
     1621 agatactgta gaatttgaat ttacgggtgt agattcaagt aaattaatcg cgaaagatga
     1681 attaaaaatc caaatcgttt ctccaggttt tgataaacca gaagaaggtt caaccgttat
     1741 taaggaatca ccaaaagcgg ttgataaaca aaccgtggta gttggattta aaccagatgc
     1801 taaagaatca attcggaata ataaaaactt acctgaagat gcagagtatt catggaaaac
     1861 agagcctgat acttctaacg ttactgatag tacgaaaggt attgtaactg ttaagatcgg
     1921 aaatcgaact ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa
     1981 tgatgcaaca tacgtaccta taacaacaac tccagaaacg acagttcaaa gtggaaaacc
     2041 tacatttgat aaaccagatg ttcctttagc taaagatgcc ttttcaattt cagatgtcta
     2101 taataagtac ttcggtaatg caagtgttga tgcaaacact ggtattgtta cattcactcc
     2161 agctaaaggt gtaggagaat cggagccgat tactggaaca attcctatta aaattgttta
     2221 ccaagatggt tctgtaggca cgaccgattt agcagtaact gtaagtaaag atatttatga
     2281 aaatccagga gaaaacattc ctgcaggcta ccacaaagta accttcaccg cagg
//
LOCUS       DACDID010000226         2297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDID010000226 DACDID010000000
VERSION     DACDID010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.232"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1296)
                     /locus_tag="IV236_002627"
     CDS             complement(<1..1296)
                     /locus_tag="IV236_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772783.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTY"
     gene            complement(1315..1719)
                     /locus_tag="IV236_002628"
     CDS             complement(1315..1719)
                     /locus_tag="IV236_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8772784.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1951..2208)
                     /locus_tag="IV236_002629"
     CDS             complement(1951..2208)
                     /locus_tag="IV236_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8772785.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          697 a          445 c          319 g          836 t
ORIGIN      
        1 ataggtggga tatgttaaag ctgaattatt ttggatatca acattctttt gtctaaaata
       61 tctatcatat ttctcaaaaa gctgctgctt atcagtgtct ttcttccacg tttgataaag
      121 atactctata agttcgttga tattaaaaac ttcgtctact ttttccaaat catccatttg
      181 aatatatttt aattcattct gtcttttcat aaccttcatg ccgttgaaat tcacccattg
      241 tggtaaatga tacatctgaa tataaagttc ttccaaatca tctaagaaat aaaaggcatt
      301 ggcacaggtc aattgaatga tatttttcaa attgccaaca ttttcactaa agcgatagtt
      361 taataaaaaa tctaataatt gttgagatat cagtattttc tttgctagtc tttcttcttc
      421 tgatttaaaa aatatcttga ttaactgttt tctttcataa agagaacgac tgtctaatgt
      481 tggaagtgtt agctttacag gtattcttct caacaaagtt gataatagtt tatcctcagg
      541 tggttctgat gttgcaaata taatccagca acttgatgtg taccagtttt cattatcacc
      601 catccgatga aatttcctcg tatccataaa ctgaaaaagt ttctcctgac attcattgct
      661 caatgcatga atttcatcta aaaataagac tccattatta gctaattctg ctaaaccctt
      721 tcttccatct tcggctcctg tatatgcacc ttttacatgc ccaaaaaaat tggacaagaa
      781 aagttctgga ttattggcat attcactaca attaactgaa atgaaagatg ccttctcttc
      841 tatataatta ttggccaaca aatccttata cacataattt gctaaatagc tttttccagt
      901 tccagtctcg ccatgaagta aaatgggtaa gccatttggc ggatagtaga ttgctgcttt
      961 aatttgagca attacatttc gcagacttcc ttgacttccg ataacccttt ctattccaac
     1021 cctaaaagag ctatcatacc atttttccgc ttcctctgaa gagctaaaaa tttgttgatt
     1081 tggaactttc tccggtaaag aaaagaagat tactggacga gtattgattt ttccaaattc
     1141 tccatctttt acgaattcat tcaaatattg agagacaata ttacgactaa catttaattt
     1201 ttcagatatt ttctgtgctg tattgttccc ccatttattt ttaatctcag agttctctat
     1261 gacataattg acaatgcgtt cttttaattc ttccatcaca gcacctcctc gacactattc
     1321 gaattttgcc gacagtattt ttccataaat atgacttgct aaaaaggctt tgtttagatc
     1381 attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg
     1441 cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc
     1501 atctgaaagt aattcacgat acaacatcgt ttgtttgctt actgtgtcat attcctctaa
     1561 ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa
     1621 aatcagcccc aataaataat ttttatattc tgacgcatcc attttactac ggagattatc
     1681 tgccgcacta aataacttct gatttaattc tgcacccaaa aagatacact cctatctctt
     1741 atttaaagtt gctatatacc ctcttatttt agcatactca gttttagaaa aagcaaaaaa
     1801 ataaagaaac ttattcttga taacaagcta cgtgacgtat atccctaaca caaaaatagc
     1861 catatccttt ggtttggata cggctatttg atattactca ttcaattatc aaatcacagt
     1921 aggtgtgaat ctcatcggga tcggcaatat ctacttctga ttaggatcaa aggtgatttt
     1981 ctcaataaag gtgtgataca aagctttaac ttgcttcttc tcactctgga taagaaagca
     2041 atccaaacta gtcaatatct gttggacatc tttgacttga atcggttccc cttgttgttg
     2101 aaagatccct aagatttcat tctctcgaat ttgtaactgt cttcgttttt cagtcaattc
     2161 atttaaacga tcttttaaca tcggaaacaa ttcgggacta tcttccaaag cagcttcctg
     2221 tttttgctct aatagttaga ttttgcgtac cattttcttc ttttattttc cttcgattgc
     2281 tgattttaat atagtct
//
LOCUS       DACDID010000227         2285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDID010000227 DACDID010000000
VERSION     DACDID010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.233"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..890
                     /locus_tag="IV236_002630"
     CDS             273..890
                     /locus_tag="IV236_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772786.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            931..1230
                     /locus_tag="IV236_002631"
     CDS             931..1230
                     /locus_tag="IV236_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772787.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1264..1431
                     /locus_tag="IV236_002632"
     CDS             1264..1431
                     /locus_tag="IV236_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8772788.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1476..2081)
                     /locus_tag="IV236_002633"
     CDS             complement(1476..2081)
                     /locus_tag="IV236_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772789.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          764 a          367 c          425 g          729 t
ORIGIN      
        1 taaaatatac catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga
       61 ccgttaaggc ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt
      121 aaacttactg tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat
      181 ggcataataa ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa
      241 caattttagt gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca
      301 gtagttatga tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg
      361 aagaatatat atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat
      421 taatgaaacg gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag
      481 gcagaaataa acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg
      541 atatttctgt gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt
      601 tagagacgct tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc
      661 ggaaacgaat taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa
      721 aattcggcag aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga
      781 aagcaggaaa gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct
      841 tttatcgacg ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac
      901 agctattaga cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt
      961 actggaacgc tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac
     1021 ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct
     1081 aagtcgttgt ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt
     1141 gcgttaggtc atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa
     1201 cagttgaaag aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt
     1261 tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat
     1321 attgcttttc acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat
     1381 tacttttact gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat
     1441 agtgcggtag aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt
     1501 ttataaatag ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct
     1561 tgctttaaca tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta
     1621 atagaatact cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct
     1681 tgccgctgac gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat
     1741 aaaaacatat cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac
     1801 tgtgcatcta atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt
     1861 atatcttcat aatctcttcc taaacgatct agtgatgtaa cgatgacgca atcaccttca
     1921 cggatataat ttagtaattt ttgaaactca ggacgctcag tatctttacc actggctttt
     1981 tcttggaata atttatcaat gttaagtcct tccatcattg ccaattgtcg atccaaattt
     2041 tgatcttgtg aagatactcg aatatagcca atttttccca taaaaaactc ctttttaaaa
     2101 gtgatataat ttacatgtaa aatttacttg tagttttact gctatcatag catttttaaa
     2161 aataaatttt atgtagactt aaaatttact tcatggaggg tttaaatgga tgaaatcaaa
     2221 agtaacaaaa aagcatttgg catttattta ggagaaccta gtacagaaca attaaaccaa
     2281 ttttt
//
LOCUS       DACDID010000228         2281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDID010000228 DACDID010000000
VERSION     DACDID010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.234"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..1730
                     /locus_tag="IV236_002634"
     CDS             117..1730
                     /locus_tag="IV236_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8772790.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          714 a          405 c          445 g          717 t
ORIGIN      
        1 caaattattt ctctatattt tctgtctatt caaaataaac aataattttc cggcacaatt
       61 atttgctgtt ttttaccata acatagtacg atcaacgtag aaaaggagat gtttttatga
      121 aagttgttgt cattggcggt gtagctggcg gtccttcatt tgccactcgt ttccgtcgat
      181 tgaatgaagc gcacgaaatc attatctatg aacgcggaga aaatatttct tatgcaagtt
      241 gtgctttgcc ttattattta ggaggtgtga tcactgaccg tgactcgctg atcgaacgta
      301 caccagaaat attgaaaaca aaaaacaaca tcgatgtctt tactaaacac gaagtaacag
      361 caatcgatcc ttctactaag cgattaacag ttaaagacct atccacaaat gaagaaataa
      421 aaacagatta cgataagttg atcatctctt ctggtgctag accagattat ccggatattc
      481 ccggagtttt tgaagcagaa aacggctttg tcctccgtag tgtgacggat gcggatcgaa
      541 tcaaatcgtt ccttgaagaa aaaaatccac aacatgtcgt cattcttggt gcaggtgtta
      601 tgggtctgga attagctgag aatttcaagc atcgcggctt agacgtgact ttaatcgatc
      661 aattgccaca agtcgctttc ccttatgatc cagaaattgc taatttagtt tatgacaaat
      721 tgctgaaaga aggattagcc gttcatttag aaacaagagt tactgagatc cgtgataaag
      781 gtcgagaaat agtattatca gatggctccg tcctttctgc tgatatgcta atttttgctg
      841 ttggtgtttc tccgaagaat gaagtggtga aagcaaccgg catacaatta tcagatacag
      901 gtcagatcat tgttgatgac cagttacaaa ccaatctgcc ggacatctat gcgattggcg
      961 atattatcga aacgactagt gtagtgactg gtcagccgat ccaaagtatg ctttccagtg
     1021 cggccaatcg tcaaggacat atgctggcag atattttaaa tggtacgcct atgcgctatc
     1081 gcggatatat tggtgcaggt gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa
     1141 tgacagaaca tgaattaaaa gcatcaggca taacaaatta taaaactgtt tttatcactc
     1201 cctttgacca tgcctatttc tatccagaag ctacaagatt aaatctaaag ctgatttttg
     1261 atgcagatag cggtcgtatt ttaggtggac aagcatttgg agaaaaaggt gtcgataaac
     1321 ggatgggaga actttctgta gcaatcaccg gaaacttgac agtctttgat ttgcctgatt
     1381 tggagttgcc ttactctcca ccgtattcta ctacccgtga cccgttgaat atagctggtt
     1441 atgtcgcaat caatcaaatg acgaatatcg tagaaacgat caaagcaagt gatatacccg
     1501 aaaacgattt gaaagaagcg ttctttttag atatacgtga acctaataaa gcaccttctg
     1561 gcagtatctc ggcaacaaaa aatattccaa tgaatgagtt acgagatcga atcaatgaaa
     1621 tcccaaaaga taaaaaagtt tacattactt tcagaagagg attgaatact tatacttctg
     1681 cccgaatctt ggcaggtttg ggtatcaaag cgattttgat tgaagaataa aaaaacgacc
     1741 gcggtgggta gccgcggtct aggagttatt tttacttgag gagtaaaaat gaaaaagtat
     1801 atagggttgt tggttggtat gtatatagaa tagaagataa atgtgaagaa attgtgcatt
     1861 atttcttcac atttattagg ttatagttta agaaagttta aacattgcgc aatttaatta
     1921 ttttagctgt ttcttttcat aaaaaaacat gttgtgactc tgagacaaat catagtccat
     1981 agtgatgtct ctagagtaac tacgcctaat attacgaggc atcatattaa tctatcagca
     2041 ctagtaccta aactagcatc cctttcgaat attttttctt atataataaa gtttgctttc
     2101 actcctctaa tctcaccaaa tcgactggct tgcgttgtgt gatactagga actgattggt
     2161 ttagaacgac ttttctattg taatcatatt tttcgccatc ctcgttgtct tctaatcctt
     2221 caatgaatga tccctaatgt gtgtattaaa taatttaatt tttttgttcc tatttattta
     2281 a
//
LOCUS       DACDID010000229         2277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDID010000229 DACDID010000000
VERSION     DACDID010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.235"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(73..2157)
                     /gene="cadA"
                     /locus_tag="IV236_002635"
     CDS             complement(73..2157)
                     /gene="cadA"
                     /locus_tag="IV236_002635"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8772791.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          655 a          514 c          356 g          752 t
ORIGIN      
        1 tagttatttt ataatcctat ctattttctt tacatttttc attaagaaaa ggatataaaa
       61 aaaagaggac gttcattcgt cctctttttt tacatgcaag cttcgcatgg cattcaaaac
      121 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
      181 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tttgtttgac
      241 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
      301 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
      361 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
      421 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt caggaagcaa
      481 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
      541 gtcgcccgtc aacataactg tgttcttcac accagcctgt tttaattgaa ccaatgcgtc
      601 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
      661 cgctagataa agcaatgtac cgatttcttg acttaaagga aaagagattt caaatctttc
      721 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
      781 acctgcaaat tcctggatat tagttgttgg taaaagcgga cctgcgtagc cttctaaaac
      841 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaatatatt gcatgaattt
      901 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
      961 ggttttatcg aataccactg tctcggtttg cgcaagcaac tccaagtaat ttgaaccttt
     1021 tactaataca ccgattttac ttgcgcctcc gataccgcca aagaaactaa gtggcacgga
     1081 aatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     1141 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     1201 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     1261 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     1321 tttttctact tgaactaaaa atggtttatt tttattgata aatccgctta atactgtttc
     1381 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     1441 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     1501 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     1561 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     1621 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     1681 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     1741 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     1801 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     1861 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     1921 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     1981 tacttttttt gtttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
     2041 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
     2101 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
     2161 aatgacctcc tcgcctcaac atatgaacat ctcttcataa ctaattataa atgaatggtt
     2221 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaattt
//
LOCUS       DACDID010000230         2259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDID010000230 DACDID010000000
VERSION     DACDID010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.236"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            367..738
                     /locus_tag="IV236_002636"
     CDS             367..738
                     /locus_tag="IV236_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8772792.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(888..960)
                     /locus_tag="IV236_002637"
     tRNA            complement(888..960)
                     /locus_tag="IV236_002637"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Gln,seq:ctg)
     gene            complement(1006..1434)
                     /locus_tag="IV236_002638"
     CDS             complement(1006..1434)
                     /locus_tag="IV236_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772793.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1494..2252)
                     /locus_tag="IV236_002639"
     CDS             complement(1494..2252)
                     /locus_tag="IV236_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8772794.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          706 a          391 c          422 g          740 t
ORIGIN      
        1 ctgtcgatat ttacatcttt taaaagattt tgggctttct ctttataagg tcctaattgt
       61 tctttatgtt cttcgataaa tcgttttgcg ccatcgaaat tgccgttctt aatcatttcc
      121 ttgacttttt gaaataattc ttcttgattc attttgaaca catcctttgc tatcaacatg
      181 attttttaca tggacaaaac gagaaaaact gatttatcca cacgtcaata atatcaaata
      241 acgatcattt ttactaaaaa aacactcgat tctcgcttct aaagtaggag aaaaatctcc
      301 aacttgggac ggatggaatt tctttaactg acccatatac tgaatatata acaaggaggt
      361 agaaagatga aagtgattaa atatttgatt ggactggcgg cgattagtgg tattttgcta
      421 tttggactga aatattatac tgaaggaagt tatggagaaa tccctggtat acttgatcag
      481 ctaaatccat tagttgaaaa aggagaggtc tatgtaaaga cacaaaaacc agaggaagta
      541 aatgaatatg gtacagcaag gtatgtgcag aaagctgctg atgccaatgg gaaagaacga
      601 acggttgaat ttaatggtct ttctgtgtta aaagaaaatc attatttgaa aattagcaat
      661 aaaggagcac acatagaaac gtatgaggaa gttcctagag aaaaagttcc ggagaaagca
      721 ttagcaatta ttgggtaaat cagtttttgt attaagaata cgcccgattt tattattcat
      781 aatttaatta tgtaaactaa atacaagtag taaaaataat acaataatcc ccctgcaaaa
      841 agattgttct tttacagggg gttcattttt tcagcaagca catcaaattg ggatggcagg
      901 gttcgaacct acaattgctt gattcagagt caagtgcctt accagtttgg ctacatccca
      961 tttattcaac aaaataatca tagcactttc ctaaaaaaac gcaagttatt tttcttgatc
     1021 ggatagttgt tgctttactc gtaaaaggcg gcgtactagt ttcttttcta atccagcagg
     1081 aagggcaatc agttctgcta ctgacaagac ggtgtccaat aactgatatt ctttgacagg
     1141 ttcattcata atatgataga tgtttgctaa acgttcaata tattcaaagc gagtatactc
     1201 tgttcgagca attagaaatt tcaataaatc ggtggctttc tcgtactctt gatctgcgta
     1261 ataaacaaga gattgacgat acaatttgat ttccagttca ttgagggtag caggtctgcg
     1321 acgctcgttc cactcattaa tagagacttt accttgaaca tttcccttgc agtcacggac
     1381 aaaaataatc ggaatttcat ttggatccat ttttttatgt gtgaattttt ccattatcta
     1441 cgctcccttc aaaggtttgg tatcttgagt ataagggatt tttgtttgaa ttactagctt
     1501 ttctccaaag ttagcatctg tccacccatt gcagtgaaaa tcgccgaaaa atcgttgatc
     1561 ggtactgctc gattttttac accataggga tcgttgacat aaatattttc tttgtcatat
     1621 ccagtgacta cacaagcatg aacaagagga gtgactctta cttctccatt atttgtggtc
     1681 caagtcataa aatcagtttc atctgggact tcaaaattga tagtcacaga agtccagaca
     1741 ggatttcctt tagatagtgc ttttttgata tcatcgagtt gagcatcggt ttttggaaaa
     1801 gtgtgataat ccgtttggac gatttgctga gcaagggagg caactggggg aacatagacg
     1861 accatagccc agtctccgcc actgatatca ccaacaaatc cttcattagg atcgccatga
     1921 gtcccatctg cattgaaaac agggacgtaa tccaattgct cagctaactg gtttttagtt
     1981 gtttcataac cataaaaaga taaaagcata gaaagcgaag tgacttcaca gccattttct
     2041 aaagaaggag aatcaaactg gctctctaat ggaacatcta gtaaaacttc tgctttctct
     2101 tttgtctttt tcgttttttg tgttgaactt ttttgtgcag tgttcattgt atgttctgga
     2161 gaatggatat gtaggtaata acttgtaccg cttaataaaa gagcagctgt gccccctaga
     2221 aacaatagtt ttttgaacgt ttttttcttc atttgaaat
//
LOCUS       DACDID010000231         2242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDID010000231 DACDID010000000
VERSION     DACDID010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.237"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1475
                     /locus_tag="IV236_002640"
     CDS             <1..1475
                     /locus_tag="IV236_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8772795.1"
                     /translation="TEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGK
                     NGFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKM
                     AIVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRT
                     FYNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANP
                     MFEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMAT
                     KQELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAP
                     IKDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESY
                     NFEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKK
                     EPVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            1510..>2242
                     /locus_tag="IV236_002641"
     CDS             1510..>2242
                     /locus_tag="IV236_002641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772796.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYND"
BASE COUNT          817 a          275 c          450 g          700 t
ORIGIN      
        1 acactgaaaa aaattttttt cctttagcta aatatcaaaa atttatcaca cctttcattt
       61 tctgctatca aaaagaggac gacgaggtgt tttttgatga aattctaaat agtattgcgc
      121 gaggcggcgg gaaaaatggg ttcatgtcag caagagactc attttttatt agccctctct
      181 acggtgtcag aaattatgat gtaacgatta cagcgaattc tgagaagcag ggaaaggtat
      241 cttttaaaga agtttatgaa atggtacaaa tgcgcaaact ggaaaaacaa ttttatttga
      301 ctaagatggc tatagtcaac aatgtaacta attcgatttt tagttatagg acaaacaatc
      361 ctaagactat ggatagtgca agagatggct gcttagaatt tgatgaaatc catcagtttg
      421 aatcttcaga cctagttgat attcagcgta gtgggctagg gaaaataaaa catcctcgaa
      481 ctttttataa tggtacaaat ggtcatgtta gagaaggttt ttatgacaaa atgttggaac
      541 gagcacaaaa gatttttagt ggagagaaca agaatgatcg cttgtttccc tttatttgta
      601 aactggataa cattgatgaa atggataatc ctaaaatgtg gtcaaaggct aatccaatgt
      661 tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac agtgatgaaa gaatataaaa
      721 aactagaaga tgagccatca ggtaggcgtg aatttgttgt taaacgaatg aattttacag
      781 aaggtgatgc agaatcagat attacgactc atgaaaaatt aatggctact aaacaagaat
      841 tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga ttatgcaagt attcgtgatt
      901 ttgcaacagt gggtttgctt tttaaaattg atgataaatt tatctggcag caacatagtt
      961 ttgctaggaa aaagtttcta gatacattca aactgaaagc gcctattaag gattgggaga
     1021 aaaaaggttt agtgacaatc gttgatgagc catcaattga tcctcgtcat ttaattgatt
     1081 ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt ttgtgccgac ggatttcgta
     1141 tggatttgct gaaacctctt ctagagaaag aatcatataa ttttgagttt atcagaaata
     1201 taagaggtgt gcagtcaaaa gttgctccta ttattgaaga tggttttgca aatgaacggt
     1261 ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa taattcgttt atcaaagaag
     1321 acaaattagg gaataaaaca tttttgaaaa aagagccagt aaggcgtaaa acagatgggt
     1381 ttcatgcttt tttggcagct ttatataaac gagaagaaat aactgatatg gattatgagg
     1441 aagcatttga tatgttggat gagattgaat tttaaggtat actccttatt gaaaggggta
     1501 gttatagaag tgaaaaagag atattttcta tttgttacaa tatttataat aatagcaatt
     1561 tgtgttttga cagtattttt accacaaatt actgagtatt taaattatcg gatatttacc
     1621 acaaaagtag atgatagcgt tactagttta agtaggctag aatttataaa aatatgtgca
     1681 tctgtgtttg gtcctatttt gactgtaatg gtatttgtga atactttaaa tatgcagaaa
     1741 aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag ctaaggctga gaacgagaga
     1801 gcaaaaaaag agctgttaac acagatagat cgtgagttct attttttatt aaatatgttt
     1861 attgaaatac aaaaagatca aaatgccaaa caagctgtta ttgctttaca gaagttggca
     1921 atttttaacg aaaagggttt taatcaagac tatgcattta cttctaaaga tttatcagat
     1981 agtgaaattg ggagtacaat tgaacaatca ggaccgctga agggcacagt tgaatatata
     2041 aaatttgaaa catttgatgt aagcaagtcg atggggaatt atcaaacaaa aacaaaagac
     2101 gttgctacaa gtttacacga tcagaaagta gctatattga atgaacaata tgaaaagatg
     2161 caaatatatc ttggacggta cttcaaaatg tttcaccgaa tagttaaaac attaaatgag
     2221 tattatgacg attataatga tt
//
LOCUS       DACDID010000232         2235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDID010000232 DACDID010000000
VERSION     DACDID010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.238"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..197)
                     /locus_tag="IV236_002642"
     CDS             complement(<1..197)
                     /locus_tag="IV236_002642"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8772797.1"
                     /translation="METRDYIITHQKLIESGLSHFEKEGFQQTKIRQLCLTAGVTTGA
                     FYKHFTSKEALFEEIIQPHINA"
     gene            complement(489..722)
                     /locus_tag="IV236_002643"
     CDS             complement(489..722)
                     /locus_tag="IV236_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8772798.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(737..1687)
                     /locus_tag="IV236_002644"
     CDS             complement(737..1687)
                     /locus_tag="IV236_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8772799.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1684..>2235)
                     /locus_tag="IV236_002645"
     CDS             complement(1684..>2235)
                     /locus_tag="IV236_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8772800.1"
                     /translation="ITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIG
                     VSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTI
                     LIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAA
                     FLFVISFLISPKQKFLRKKERSL"
BASE COUNT          717 a          419 c          281 g          818 t
ORIGIN      
        1 gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta
       61 taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt agtttgctga
      121 aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg agttataata
      181 taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt attatcttac
      241 acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt agagctaaac
      301 taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt aatagactca
      361 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
      421 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
      481 aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt
      541 aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca
      601 gatgctggac taacacttag aacttttgct aaatcattat tttttaattt cttttttctt
      661 tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc
      721 atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca
      781 tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg
      841 ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac
      901 tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta
      961 tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat
     1021 tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat
     1081 tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat
     1141 acttatctag attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt
     1201 tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt
     1261 ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa
     1321 aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc
     1381 ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa
     1441 catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta
     1501 aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta
     1561 ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg
     1621 ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc
     1681 tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact
     1741 aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact
     1801 ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat
     1861 catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc
     1921 aacaattaat atagttccca cactttgcat agctgttact gctactaaag ttaatagaac
     1981 catcaataga taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc
     2041 aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga
     2101 aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat
     2161 atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa
     2221 agaactgaaa gtaat
//
LOCUS       DACDID010000233         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDID010000233 DACDID010000000
VERSION     DACDID010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.239"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IV236_002646"
     CDS             <1..543
                     /locus_tag="IV236_002646"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8772801.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="IV236_002647"
     CDS             complement(899..1471)
                     /locus_tag="IV236_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772802.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="IV236_002648"
     CDS             complement(1487..2092)
                     /locus_tag="IV236_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8772803.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DACDID010000234         2218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDID010000234 DACDID010000000
VERSION     DACDID010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.240"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..324
                     /locus_tag="IV236_002649"
     CDS             <1..324
                     /locus_tag="IV236_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8772804.1"
                     /translation="GLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRI
                     ACARVLLKNSKIILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGN
                     KKIML"
     gene            352..780
                     /locus_tag="IV236_002650"
     CDS             352..780
                     /locus_tag="IV236_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772805.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            1237..2148
                     /locus_tag="IV236_002651"
     CDS             1237..2148
                     /locus_tag="IV236_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8772806.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          865 a          326 c          435 g          592 t
ORIGIN      
        1 ggattgaagt tttcgaattt aaatgtttca gataagcgaa cagcaaagta tgaggcattg
       61 aaaaaagtaa atataaacaa gaagctaagt acgcctatct acacattatc tggtggtgag
      121 aagcaaagaa tcgcctgtgc gagagtctta ttgaagaata gcaaaatcat tttagccgat
      181 gaaccgactg ggtcattgga taaaagaaat aaggataaag tagttgaaat cttatcgcaa
      241 caagcttccc aaggtacgtt agttatcgta gtcacgcatg atccatatct gaccaaaata
      301 ggaaacaaga agatcatgct ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa
      361 gcagttatta tttgtgcact agggataggt tttgttggag gaaacgctat gtcatctgaa
      421 gtggaagcaa attcagttca cggtgaaatc tattacaaat ctttaaagcc aggagagatt
      481 cattttgatg ctaaaggtaa tattattaag tcaaatttac cagtagaccg ggaaatttct
      541 agaacagcaa ttaatcatag ctctggaaga tgggtttatt atagtgatac aacttgtacg
      601 agaaaaacag gtcattctaa ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg
      661 aaagttggaa actctattcg atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt
      721 gcgacaggaa atagaactga cacattctca tgttggtaca atccaaccgg ttggtattaa
      781 gtatccaaaa gtttatataa aatagaaaaa acatataatg aaacagcgtt aaaagttatt
      841 tttggtagtt ttaatatatt catttttagt tgtataaaaa tatatcataa taaaaacaac
      901 aaaaaaatgt ctggaaaatc gaacataaaa gtgtctgatt tgaatatccc agcagcggaa
      961 gaagaaaaac aaaatcgata acatttgttc taagaattag ttacgttaaa tacggaacgc
     1021 aagatagact tgaaaaacgg taacaaaaga agtagtatat taaggtgtga tgtgctactt
     1081 gacatttatt ctgctgagcg aactttctgg attgtgtagc cttatatcta ttcaggacgt
     1141 atcgagcaga ttagtaaagg tgttcttgat gattttcatc ttgaacacct ttgtttttag
     1201 caattcgcaa aaaacgaaaa gtaggagaat tgataaatga aaaaaagatt tttagcactt
     1261 gccatcgtac taggcactgg cttattgtca ggctgtacga atgccggtga aaaaacagcc
     1321 gttagttata aaggcggtac aatcagcgaa caagaagtaa tggatagctt gaaaaaaatg
     1381 caaggagcag attccgctgt gcaacagttg atcgtttatc aagtatttga agataaatat
     1441 ggagatgacg tatcaaccaa agaaatcgat tctcaatatg atcaaacaaa gaaacaattg
     1501 ggtgattcat ttgattcaca attgaaatct gcaggttaca cagaacaaac attcaaagac
     1561 agtatcaaac aaagtctagc ctttcaagaa ggtctaaaga aacatatcaa attgaccgat
     1621 gaagatttaa aaacggcttg ggaatctttc catccagaag tggaagctca aattatccaa
     1681 gtagcaagtg aagatgatgc aaaagatgtg aagaaagctg ctgataaggg agacgatttc
     1741 tctaaattag ccaaagacaa gtcaactgat actacgacaa aagaagatgg cggaaaagtg
     1801 aaatttgatt ccactactac aacggttccg gcagaagtca aagaagcagc ctttaagttg
     1861 aaagatggtc aagtttctga tgtgatcact tctaccaatg cttcaactta tactacggaa
     1921 tattatgtgg ttaaaatggt gaaaaaccaa aacaaaggca acgacatgga taaatacaaa
     1981 aaagaattga aagaaattgc aacagacaca aaattaagtg acagtacctt ccagaataaa
     2041 gtaattggcg aagtattgaa agacgctaat gtaaaaatca aagacaaaga ctttgaaaat
     2101 gttttgagta cattcacttc agattctagt acagcaagta gtaaataagc ggtataaata
     2161 aaaaatatga actatttaaa tcaataaaac tgttcatcta aatcttttta ttagaact
//
LOCUS       DACDID010000235         2209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDID010000235 DACDID010000000
VERSION     DACDID010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.241"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..417
                     /locus_tag="IV236_002652"
     CDS             <1..417
                     /locus_tag="IV236_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP8772807.1"
                     /translation="IIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDA
                     VIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGS
                     WCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            407..1549
                     /locus_tag="IV236_002653"
     CDS             407..1549
                     /locus_tag="IV236_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8772808.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1562..>2209
                     /locus_tag="IV236_002654"
     CDS             1562..>2209
                     /locus_tag="IV236_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8772809.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHE"
BASE COUNT          782 a          296 c          436 g          695 t
ORIGIN      
        1 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
       61 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
      121 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
      181 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
      241 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
      301 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
      361 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
      421 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
      481 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
      541 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
      601 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
      661 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
      721 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
      781 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
      841 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
      901 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
      961 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     1021 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     1081 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     1141 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     1201 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     1261 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     1321 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     1381 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     1441 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     1501 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     1561 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     1621 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     1681 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     1741 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     1801 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     1861 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     1921 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     1981 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     2041 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     2101 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     2161 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaa
//
LOCUS       DACDID010000236         2187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDID010000236 DACDID010000000
VERSION     DACDID010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.242"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            115..948
                     /gene="msrA"
                     /locus_tag="IV236_002655"
     CDS             115..948
                     /gene="msrA"
                     /locus_tag="IV236_002655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8772810.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1028..2017
                     /locus_tag="IV236_002656"
     CDS             1028..2017
                     /locus_tag="IV236_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8772811.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          789 a          363 c          436 g          599 t
ORIGIN      
        1 ctctttttta tacgaaaaat ctgtaggggg atgaagtaat cgcttatttc ctcactttat
       61 gataaattac agctaaagtc actagcttgt cttcttcgga aaggaggaga atctatggat
      121 gaggaacaaa tattcattca aacactttat aatctgattt tgaatccgaa tacacgagat
      181 tgggaacgaa aagtattgat tcagacaaag aatgacataa gagaaaatat atccgttaaa
      241 gaacagttat caaaactaga agcaacacta cgtccattag ctatccgaat gaatctgacg
      301 cctgatgtaa tggactttta tttattgatt acggaaggat ttgataagga gcaaaaatat
      361 gacttttcca agcatgccat gcaagatgct gactatcaag aacgtgcagt atttgcaggg
      421 ggatgtttct ggtgtatggt cgagccgttt gaatcgaaaa aagggatcct atctgttttg
      481 tcaggataca cgggcggaca tgttgaaaaa ccaaattatg atcaagtaag tggtggttac
      541 acaggtcatg tggaagctgt ggagatcatt tatgacacaa gagaaataag ttatagcgag
      601 ttgctgacaa tctattggca aattactgat cccacagata catttggaca atttcaagat
      661 agagggaagc aatatcgtcc agtgattttt tatcaagtcg aacgacaaaa agaattagcg
      721 gaacaatcaa aacagaaact agacagttca ggaactttcc accaaccaat cgtcacaaaa
      781 atcgaaccag ccggtacgtt ttggccagca gaaaactatc atcagcaatt ttacaaaaaa
      841 caaccgaaac gttataaaaa aatccagcaa gcacgtaatc aatttctgat ctatcaacga
      901 gtaaaaaaca aatggcaaaa aaatatacga aaaaatcatt ttgattaact ccttttcttt
      961 aaaatgtagt aaactaaaaa tagaaaaaag aaagcgtttt aaaaagattt gatgaggagg
     1021 agaaaaattg tatattgcag atagtgcacg ttatgaaaaa atggaatatc gtaaatccgg
     1081 tcattcaggt ttgaaattac cgattttatc tctcggatta tggcaaaatt ttggtgatta
     1141 tgatcctatc cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca
     1201 tttcgacctt gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg
     1261 tatttttaga gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg
     1321 ttatcatatg tgggaagggc catatgggga atggggctct cgtaaatcca tcatttcaag
     1381 ttgtgaccaa agtctccaaa gaatggggct agattatgtt gacatcttct atcatcatcg
     1441 cccagaccct gatacacctt tggaagaaac agcagaagct ttgatgcaac ttgtaagaca
     1501 aggaaaagca ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaaatgac
     1561 tgaaatttta aaaagaaagg aagcaccgtt cattattcat cagatgcgct acaatatgtt
     1621 ttctcgtgca ctactagaag acgatctatc tccagtatta gaagaagaag ggctaggggc
     1681 aatcacgttc agtccgcttg ctcaagggtt gctgacaaat cgttatctac atggtattcc
     1741 tgaagattct agagcacatc gtaaggaaat accattcctt tcagaagaac aagttggatc
     1801 aactctggaa aaaatcaaag cactgcaaac aatcgccgta tcacgaggac aatcattagc
     1861 gcaaatggcc cttgcatgga atttgcgaca aaaatctgtt acaagtgtac tggtcggagc
     1921 aagtcggtta agtcagttac aagaaagtgt tcgaatgatg gataatttag atttctcacc
     1981 agaagaagaa ttgagaatcg accaaattct agaatagaaa cgatgtaatc aaacttttta
     2041 tccatatatt aaaaaaaaca aaagatgaca tcaagaatcg aatgaagatc gtctgttatt
     2101 gaacatcata ttttttgtcg ttcaaaataa aaatgatcga aggctaggag tgaattttat
     2161 cctagtcttt tttatggtaa atatctt
//
LOCUS       DACDID010000237         2168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDID010000237 DACDID010000000
VERSION     DACDID010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.243"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            140..406
                     /locus_tag="IV236_002657"
     CDS             140..406
                     /locus_tag="IV236_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772812.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            464..976
                     /locus_tag="IV236_002658"
     CDS             464..976
                     /locus_tag="IV236_002658"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8772813.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            973..2010
                     /locus_tag="IV236_002659"
     CDS             973..2010
                     /locus_tag="IV236_002659"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8772814.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          783 a          401 c          444 g          540 t
ORIGIN      
        1 tgagcaaaat aaacagtcga tgtttgtttc ttaattgtat atcttgatat taaacgattt
       61 aagattattc ttcaagatat atattcgcaa atgtaattgg aaaagcgact tcttaaacaa
      121 aattaaggag tcgcttttta tggcaaaaat aacgaaaaat gagcaagaaa atttggtgga
      181 aaattacaca aaaaaaccgg cgtactctaa tagccggtta gacgcacatt tcgtgtgcac
      241 ttctaatccg aaattgagct ttgacgctat gacgatagtc ggaaacctta ataaaaacag
      301 cgctaaaaag ctatccgatt ttatgagttt agatccacaa attagacttt gggatatact
      361 tcaaacaaaa tttaaagcta aagcactaca agacccccta tgttaggcta gttgtcgtat
      421 aggataataa gttataataa agactatacg gaggatgatt accatgacaa ataaaaacaa
      481 tgaacgttac tctaccgaac tgaaagcgac agccatcaag aaaatgatgc cacccgaaaa
      541 tagggggatt cgccaattaa gtcaaacttt gggcattcca gaagcgactc tttattcttg
      601 gcgtaaaaaa gctagaattc aagggaatac tacacccgga acgaaaaaaa atgtcgaaaa
      661 atggtcaagt gaagataaat ttttaattgt gatggaatca ttctcaatga accaagtaga
      721 acttgcggaa tattgccgaa agaagggctt atttaaagaa caaattgagg cttggaagaa
      781 aacgtgctta tctgcgaatg aacaggaaga aaatcgaacg agagaacttg caactgaact
      841 aaaagaagag aaaaaacaag cgcgtcaatt agagaaagaa ttacgtcata aggaaaaagc
      901 attagcggaa gccgctgctc tcttattact aagaaaaaaa gcacacgcga tttgggggga
      961 ccaagaggaa gaatgatttc atcttcagat cgcaaacaag ctgttgaact tattcaagaa
     1021 gccaatcgca atggggcccg tctcacctat gcctgtaatg agctgaatat tagcgttcga
     1081 acctacgaac gatggacgcg tgaaggtacg atagcgcatg atcaacgccc tttggcgaaa
     1141 cgtcctgtac ccaaaaataa actgacggat caagaacgcg aaaaaataat agaaaccgtt
     1201 tcaaaaaaag aatttatgaa tttgccacct agccaaattg taccaaaact tgcggattgc
     1261 tcgatttata ttgcatcaga gtctagcttc tatcgtgtat tgcgagaaaa aaatatgcaa
     1321 catcaccgtg gccgaagtca agtttcacaa aaacgcatac cgccaagtca ccttgcgacg
     1381 aagccaaatg aagtttggac ttgggatatt acttggctaa aaggtccaat aaaaggactc
     1441 ttttaccggt tatatctcat cattgattta tttagccgta aaatggtagg ctgggagatt
     1501 tgggaatcag aagaagctcg tcatgccgaa aaacttatta aacagacggt gcttaaggaa
     1561 cgtatccaag gacagccgtt ggttttacac tccgataatg gcagccctat gaaagccgcg
     1621 acttttcaag tcttactcga aaaattagga attcaaagtt cttattcaag accacgagtg
     1681 agtaatgaca atccttattc ggaagcggct ttccgaacgt taaaatatcg accagaatat
     1741 cccaataacg gctttaaaac aatagatgat gctcgaaaat ggacgaaaga gtttgtcgat
     1801 tggtataacg attctcattt gcatagtggg attcaatttg tgtctcctgc agcgtgtcat
     1861 gaaggacgac atctcgctat tttagagaaa cgaaagcagg tttacgagga cgctaaaaag
     1921 cgccatcctg aacgctggtc aaaaggtgtt cgaaattgga ctccacatca atcggtggcg
     1981 ttgaatcctt taaaagaaac agaaagttaa caggagcata atctttcaag attatcaaat
     2041 aaccctcgtc acccaatagg ggaaaaagtt attcttcccc tattggataa cgaggccaac
     2101 cgaccatagg gaggttctgc tttttgatag gtggaaaata aaaatacgac aactatattg
     2161 acaaacac
//
LOCUS       DACDID010000238         2149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDID010000238 DACDID010000000
VERSION     DACDID010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.244"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            183..557
                     /locus_tag="IV236_002660"
     CDS             183..557
                     /locus_tag="IV236_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772815.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            562..990
                     /locus_tag="IV236_002661"
     CDS             562..990
                     /locus_tag="IV236_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8772816.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1081..1995
                     /locus_tag="IV236_002662"
     CDS             1081..1995
                     /locus_tag="IV236_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8772817.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
BASE COUNT          793 a          348 c          376 g          632 t
ORIGIN      
        1 agacacagaa atgcctagta atttacattt cagagtttgc cgaccgtgaa gtgtttatta
       61 gttatcaagt ctttttagct aatattttag cttacgaaaa cagtatacta acacttttat
      121 tagttgtcaa cacctttaac taattttttt attagtgttt tcgtagcttg tgaggtgcga
      181 aaatgaattt actagataga ataaaggaat tagctcataa aagaggcata agtataactc
      241 aattggaaga agaattaaat atacctaaaa acacgattta tcaatggaaa aatagaactc
      301 ctagtactaa aagactacaa cttgtagcag attattttaa tgtaactact gattatttac
      361 tcggtagaaa ccaagttcct gactgggcaa caaaagatga agtagttgaa cttgataaat
      421 tactagattc aaatgtcaat atgtcttatg gcggagaaac actgacgcct gaacaggtcc
      481 aacgcgtaaa agatatcctt attggaacct tctgggatat tgtgaaagaa gacaaagaaa
      541 aaggcaaaaa gatgtgagct tatggagatg gatacgatta atttagtcga ggagttgaag
      601 cggaaatacc agtccgctaa tcctttttat atttgtgaaa agatgggcat taaaattcaa
      661 tacgttcctt ttatcgagaa tcccaaagga cagtttcaag aaattagagg tcgtgcaata
      721 atcttcttaa atgatgaact gagagactct gaggaaagat tctacatttg cgctcacgaa
      781 ttaggtcacg ctatttttca tcgtggctta tccagttact atgtatcaac aagaacatct
      841 agaagcaaat ctgaaagcga agctaattgc tttgctgcta atctcattgt ttctctatac
      901 aaagaagaca atgatcaata tcctaaacga attgaggaat taaagaatct ttacggactt
      961 ccagaaagcg cttatcgctt tcttatataa tgtaaaaaaa gtccgtgctg caacacggac
     1021 taaaaacctt atttctaaga tcctacaaaa aaatcatatc atagaagtga ggaattaaaa
     1081 atgaaaaaaa ttgtttttgg gttgcttttt ttaggcttgg tattaagcgg atgctccact
     1141 agtcctaatt ctgctagcac taaagaaaat actgtttcat caacatctga atatgaaaca
     1201 aaaaaactaa cttcatcaga cgttcataaa ataaaagttg gtgatgattt aactcaagtt
     1261 tatcagacac ttggttcacc gttacgtaaa tggacaaatg attttgttta caaagaatta
     1321 gacaatgcta tcacacggga tgaattaatt gtatctattt caaaggatgc acaaggagat
     1381 tattataaag agcactctga attaattgaa catggaaata gtgcaaaaga tataaaaaaa
     1441 ttagagatgc tccaatatac ttattcaact gattctgatg atgaaacaat gttaatttgg
     1501 gttagtccaa aaactgaaca ggttgtatat tataatcaac gaactttctt agataaagat
     1561 ggaaaatcca aagattcaag ctctactaca actacaacta caacttcgtc ttcagaaccc
     1621 cagacggcat cagttgcaca tagtatagga gaagcagttc cttttacttc tgaaaatggt
     1681 aactctatta acgtaactgt ccagtctgta tcaaaagatt acggtgatga tttctataaa
     1741 ccagaaggcc tattttacgc aaaggtagat tttcttgtag aaaacacagg gactacacct
     1801 tttgatgcaa gttcaagtta tttagaattt tatgattcat cagatattaa atctgattta
     1861 gctagtagag attacttttc ggaaaccatc caaccgggta aatccgcaca aggaacagca
     1921 tattttgaag tgaaaaatga tggaacaaca tttgaagtgt tcttcgcaga tacttcatgg
     1981 aaaggtagct actaaacaaa aaaacacgcc ccctccctcg ccaaagtttg tggacgtgat
     2041 gaaaaataaa cctgctataa ttggcttact tatccattcc tattatagca acaaatagga
     2101 gatgaaaaca atgtggattg aaaccaaaac tgataaaaac ggaaaaaaa
//
LOCUS       DACDID010000239         2145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDID010000239 DACDID010000000
VERSION     DACDID010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.245"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1278
                     /locus_tag="IV236_002663"
     CDS             <1..1278
                     /locus_tag="IV236_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772818.1"
                     /translation="DIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISL
                     LVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYP
                     MLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILK
                     EMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLV
                     FMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSD
                     MGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPV
                     YAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYC
                     LVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1265..>2145
                     /locus_tag="IV236_002664"
     CDS             1265..>2145
                     /locus_tag="IV236_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772819.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIP"
BASE COUNT          715 a          329 c          372 g          729 t
ORIGIN      
        1 gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat cccagctgtt
       61 ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc tttatctgtc
      121 agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag tgcattgatt
      181 gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga tataaaattt
      241 gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag ctatttttgg
      301 tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa aacagatttg
      361 cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa cgatattaca
      421 acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt catactgaaa
      481 gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg ggtatgcgta
      541 actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact ccagtttcct
      601 tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct tgtttttatg
      661 aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt ggtgtttcta
      721 ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc aaaacacggt
      781 catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat gggaggatta
      841 tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa acatgaagta
      901 aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa cattacttac
      961 acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta tttaggtaca
     1021 caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg cttagtgaat
     1081 gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac tccaactgct
     1141 ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc aagttttaaa
     1201 cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga gataaacaag
     1261 aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag ttagtagtat
     1321 atattctttc atctttttca aaaatacaca gagtatttat accactggag atctttcttt
     1381 tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac
     1441 attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc
     1501 tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt
     1561 attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa
     1621 aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat
     1681 ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt
     1741 ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt
     1801 gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat
     1861 tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc
     1921 tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg
     1981 gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt
     2041 atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac
     2101 tacttactca atgggactag ttggtctgtt atctttatgt attcc
//
LOCUS       DACDID010000240         2127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDID010000240 DACDID010000000
VERSION     DACDID010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.246"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            152..670
                     /locus_tag="IV236_002665"
     CDS             152..670
                     /locus_tag="IV236_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772820.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            678..1718
                     /locus_tag="IV236_002666"
     CDS             678..1718
                     /locus_tag="IV236_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8772821.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1967..>2127
                     /locus_tag="IV236_002667"
     CDS             1967..>2127
                     /locus_tag="IV236_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8772822.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          748 a          257 c          431 g          691 t
ORIGIN      
        1 gtccgtattt aaatatcatg cttaacaatc tggaaaaact tcagttgcaa aaaataagta
       61 tgttattgtt tataggtgta tgctgttatc gtatgttatt taaaacatct ttgtttgggg
      121 attgtgctgt tttaattgca ctatatgtca tatgttatac actgaaaaaa acgataataa
      181 tatttttgag agacacagaa tcataggaac aattgtatgt ataacagtta taccacttct
      241 catatccata ggaatacttt tcaaatttat accaaatgca atggttaacc agatggttca
      301 tattggttct ccattgatgg ttatagcaag tatattttta ttttatgtat ttaaaaattt
      361 ttcattttac agtaagtata taaataaact tgcgagaacc agtttagggg tatatatttt
      421 acatgaaaat cattatttca ttgatggtat gaagtctaga ggcttaatat gggatgggat
      481 atttggatta ggcaaaaggt ttttagatag ggatttttgg atttggttta taattgcagt
      541 tgtagttgtg tgcataattt gcatggctat cgatgttgta agagaaaaat ttgaaagaca
      601 gatttttaaa attaagagtg taaagaacct gtgtaaactt atgaatcatg ttataaatat
      661 atacccataa ttaataaatg aggagaaaag aagtgaaagt tatatcattc ggagaattat
      721 tattgagact tgcagcaccc ggttattcaa agttatttca aagagatagt ttggagacta
      781 cattctgcgg aggggaagct aatgtagcgg tatccttagc aatatttgga tatcaatcag
      841 aatttataac aaagcttccg gattcagatg ttggatatgc ggcagctaat gctttgcgtt
      901 attttggagt aaatgttgat aatattatat ttggagaggg acgcatggga ctgtattatt
      961 tagaaaaagg agcagctcag agaccatcta aagtaattta tgacagaaaa ttttctgcaa
     1021 tttcgttagc tgatagtgat gaatttaagt ggaaggagat cctaaacgat gcaaattggt
     1081 ttcattggac agggataaat ccagcattgt ctgagaatct ctttgagacg tccttagatg
     1141 catgtcaggc tgccaaaaaa agaggtataa caatttcttg tgatttaaat tatcgatcca
     1201 agctttggac atcagaggaa gcaaaagaaa agatggaaca gatactacca ttggttgata
     1261 tttgtatttg taatgaagaa gatgcagaaa aagtgctagg aattaaagct gagcatacaa
     1321 atattaatag tggagaatta aataaagatg gctatgtgca ggtggctagg gagattcaca
     1381 agaagtttgg atgtaggtat gtagctacaa ctctgcgtag aagttattcg gcaagtagaa
     1441 atggttggag tgcaatgtta tacgaagcag aaaaagataa agcgtatttt tccacagaat
     1501 acaacataca aattgttgat cgcgttggtg gtggagacag cttttctgct ggtataatat
     1561 atgcgttatg tgaaaatatg tccggacaaa aggctattga atttgcgact gcatctagtt
     1621 gtttaaaaca tactatagaa ggtgatttta atcgaactac aattgaggat gtatataatt
     1681 taatgaatag cggtggaaat ggtcgcgtgt tgcgttagtc aacatataaa gataaatcac
     1741 agtaattttt atggtgatta tctgtcttag tagtgattag aataacgtga aaataaattg
     1801 ttgagcgttt atactgtttt gaaaaataaa tttaacatat tgaaaaatac aaatatatac
     1861 gtatattata aagcgccgat tgtaaaatta agctagacaa ctaaaacagt catttgtgct
     1921 acactcaaaa tagttccgac caaagaatta taaggagtga gtgcaaataa cctataccta
     1981 tcttacaacg gatgaacttg taataataga gtcttatttg aaaattaacc aatctgttgc
     2041 taaaactgcg cattgcttga atcgttcaag tcaaacgatc cataaagtat atctgttctt
     2101 caagcaaaga aaatcagcct tagaata
//
LOCUS       DACDID010000241         2120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDID010000241 DACDID010000000
VERSION     DACDID010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.247"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV236_002668"
     CDS             complement(<1..914)
                     /locus_tag="IV236_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8772823.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV236_002669"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV236_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8772824.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          715 a          374 c          412 g          619 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg
//
LOCUS       DACDID010000242         2120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDID010000242 DACDID010000000
VERSION     DACDID010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.248"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(660..950)
                     /locus_tag="IV236_002670"
     CDS             complement(660..950)
                     /locus_tag="IV236_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772825.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1158..1850)
                     /locus_tag="IV236_002671"
     CDS             complement(1158..1850)
                     /locus_tag="IV236_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8772826.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          602 a          392 c          312 g          814 t
ORIGIN      
        1 cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg
       61 caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt
      121 agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata
      181 caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca
      241 aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat
      301 ttacttttac gactatgata atcacctctt cgagttacat accggtactc tccaggaacg
      361 tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct
      421 ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag
      481 caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg
      541 tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg
      601 tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc
      661 tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct
      721 accacaattt tgggtttccc taacacatga taggatctca caacaaattg ctttctatta
      781 ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa
      841 ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca
      901 ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc
      961 tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt
     1021 tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga
     1081 catgtactaa aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt
     1141 ttacttgtta agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca
     1201 gctgtatctt ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt
     1261 gagttttcag cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca
     1321 tttgtttttt cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt
     1381 aattctgaaa gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga
     1441 ttgatttcat catttttttg gttgatctca tctagtttga cattgatttc attttgtttg
     1501 ctttgtagtt gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc
     1561 gattgatttt tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc
     1621 tttgaatctt gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaagcgct
     1681 tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata
     1741 tctgtctctc caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt
     1801 agacatagaa atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct
     1861 ccttaatcca atacttttac atgctaaaag taagtgatct caaacacaat gtcaagcgtt
     1921 tttaaattac atgtgtaatt ttaatataaa tactatcaaa tactcattta atcctccttt
     1981 tttatctatt taaaaaatct tttttataga gaaaaatgca ggttaatact ttcttaaata
     2041 aatatttatg gtgaacaaaa acaaaaaagc aagttttatt aaacgtaacc cttttcattt
     2101 catagattac tgtatttatt
//
LOCUS       DACDID010000243         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDID010000243 DACDID010000000
VERSION     DACDID010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.249"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IV236_002672"
     CDS             169..1539
                     /locus_tag="IV236_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8772827.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IV236_002673"
     CDS             1560..1844
                     /locus_tag="IV236_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772828.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DACDID010000244         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDID010000244 DACDID010000000
VERSION     DACDID010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.250"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..618
                     /locus_tag="IV236_002674"
     CDS             196..618
                     /locus_tag="IV236_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8772829.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(784..1977)
                     /locus_tag="IV236_002675"
     CDS             complement(784..1977)
                     /locus_tag="IV236_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8772830.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT          761 a          420 c          343 g          564 t
ORIGIN      
        1 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
       61 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
      121 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
      181 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
      241 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
      301 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
      361 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
      421 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
      481 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
      541 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
      601 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
      661 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
      721 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
      781 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
      841 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
      901 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
      961 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     1021 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     1081 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     1141 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     1201 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     1261 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
     1321 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     1381 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     1441 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     1501 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     1561 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     1621 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
     1681 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     1741 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     1801 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     1861 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     1921 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
     1981 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     2041 aaacaagact tttttatttg aaataataca caaacaaccg atatagat
//
LOCUS       DACDID010000245         2086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDID010000245 DACDID010000000
VERSION     DACDID010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.251"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>2086
                     /gene="acm"
                     /locus_tag="IV236_002676"
     CDS             <1..>2086
                     /gene="acm"
                     /locus_tag="IV236_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8772831.1"
                     /translation="RNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNI
                     TDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTV
                     RKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTK
                     PASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPST
                     FEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVS
                     YKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIF
                     KYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEA
                     PNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFK
                     LFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVP
                     SGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEV
                     SEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPG
                     SHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKS
                     ILPKTSSKKSSFA"
BASE COUNT          820 a          342 c          403 g          521 t
ORIGIN      
        1 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
       61 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      121 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      181 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      241 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      301 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      361 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      421 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      481 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      541 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
      601 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
      661 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
      721 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
      781 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
      841 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
      901 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
      961 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1021 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1081 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1141 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1201 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1261 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1321 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1381 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1441 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1501 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1561 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     1621 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     1681 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     1741 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     1801 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     1861 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     1921 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     1981 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2041 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgct
//
LOCUS       DACDID010000246         2049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDID010000246 DACDID010000000
VERSION     DACDID010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.252"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IV236_002677"
     CDS             complement(<1..223)
                     /locus_tag="IV236_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772832.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLA"
     gene            537..1151
                     /locus_tag="IV236_002678"
     CDS             537..1151
                     /locus_tag="IV236_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772833.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1175..1525)
                     /locus_tag="IV236_002679"
     CDS             complement(1175..1525)
                     /locus_tag="IV236_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8772834.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1519..1782)
                     /locus_tag="IV236_002680"
     CDS             complement(1519..1782)
                     /locus_tag="IV236_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8772835.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          705 a          346 c          305 g          693 t
ORIGIN      
        1 gagcaagtaa tggagaaact attaaataat tttcagtaac agttaagtcg ttagttgggt
       61 taccaatggc tattccgttc ctatctaaag gagttaattc ttggttatta tctactacag
      121 tagttggttc atttgtacta tcagcaaatg taggaattgt agttccagtt acactaattc
      181 caatagttaa aatacctaaa gataaaataa acttattttt cattgtctat cacctcgaaa
      241 tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt tatatactag
      301 aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga ttgacattta
      361 tttacaaaaa atattgccat ttaaaaagaa aagtgacaca tactatttcc tccatcatta
      421 gtttttgtct atataaggta taccctaact agacacgacc gcgacattga aaaatctggt
      481 tttctcgtgt atgattaggg tgttttttaa atagttgcag aaagtaggta aaaattttga
      541 gagttgcata catacgtgtc tcatccatcg atcaaaatga acaacggcag attgaagaaa
      601 tgaaaaaatt cggtgcagag agaattttca ttgagaaaca atctggcgca acgattactc
      661 atcgacctgt ttttcaagaa gcactggatt tcgtccgaga tcaagatatt tttattgtgg
      721 aagctattga tcgacttgga agaaattacg acgagatcat cgcttcagtc aattacttga
      781 agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat catggccgaa gcaattggca
      841 atcctctcct ggataaattc attaaagatt tgatcattca aattttggcc atgatcgctg
      901 aacaagaacg taccgaatct aaacgacgtc aggcacaagg cattaagatt gcaaaagcga
      961 acggtgttta taaaggacgt cctaagctat acagtgccaa tgcaaaagat ccccaacgtc
     1021 gtcttgttta taaaaacatc gttgaggatc taaaaaaagg tgttgcgatc gctaagattg
     1081 caaaagacta caatgttacg agacaaaccg tatatagaat aaaaaaagac agcatggtca
     1141 atcacgaata gttgatcatg ctgctgtttc tattttattt gcttactttt tgaaaaactg
     1201 ttctaacttt catcaataac tcctcaacta aactatcatg cgctttttcc aaaaaaatac
     1261 actctctggc ttcataatca attgtcgtta gttgatctaa taatactttt cctgttgtct
     1321 tcatttcata ttctgagttt atatttacat atagcggata gtttctagtt gtactagaaa
     1381 ttggagcaac tgtaacaacg ttactataat ccgcaataat atcatgactt aaaacaatat
     1441 aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc tgtattcaat aatagaatat
     1501 cgccttgtct aggttttctc accatttttc attacctact gcttcaccta aattttgaat
     1561 cgtcacatga tttctttcat cagtgtaatt ttcaaataac tgttcaattg ttaattcatt
     1621 ttctatatta gccgcttcga taattagccg cttctgaccg tttacttctt cttgtattat
     1681 cttcactttg tcttctgtat gaattccaag atattccata atatactttg gcaatctaag
     1741 gccattacta tttccccatt tttttatagg taacatttcc atagtagaca cctctttctg
     1801 tatatacata gtataacata cataagaaaa aacgtatata cttaattatc cttttctatt
     1861 aaattagatt aacattccat gaatccataa actatgttaa catttatatg aaaatagtcg
     1921 aaaggaaatc ataatatgtt taagaaaaca aaatctttag aatcaggtaa gaaaaaaatt
     1981 gatctaaaaa taatctcttc tggatacttt aaagatttca tagttattga tacaaaatgg
     2041 aataaaaag
//
LOCUS       DACDID010000247         2024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDID010000247 DACDID010000000
VERSION     DACDID010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.253"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..168
                     /locus_tag="IV236_002681"
     CDS             <1..168
                     /locus_tag="IV236_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8772836.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVAPDDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="IV236_002682"
     CDS             150..1064
                     /locus_tag="IV236_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8772837.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1309..1995
                     /locus_tag="IV236_002683"
     CDS             1309..1995
                     /locus_tag="IV236_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772838.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTAC
                     YEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTE
                     EACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESV
                     KEWLDQQQQN"
BASE COUNT          806 a          383 c          372 g          463 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg
       61 tgcaatacac accaacacga cgttgctcct gatgatgctc aacgtttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt
      181 catgtagccg agcaatcaat tacgcagaac cacgagcaac cgtcaaaaca ggaataaact
      241 gtgacattga ttatgccaaa agtgaaatga aacaaatacg cacgctttat ggaaaagacg
      301 atcgtgtaca agcacactta ttgattcagt cctttcgacc aggagaaatt accgctgaaa
      361 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctaaccac caaatcgcga
      421 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      481 ttgaaactgg gaaaaaattt caagcccatg ggcaagcatt tttaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gaacatgact taacaatcac ggaacgagga aagaaaaccg
      601 aaaaacgaac catgtctgaa attaaattaa aagaaaaaaa tgaaccggtt tggaaagacg
      661 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tatacccaat
      721 tttgtgattc acttaaaaca ttcggcattc attgcttgaa tcgcggaaaa aattttactt
      781 acgaattggt ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgaac ctacaatcaa caacgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      961 aacgaacttt tacgcctgaa caatcggaaa ttagaccgac tcgaagaaac catgaacaac
     1021 acgttgaacg aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacatc
     1081 gaaaacgaaa taaagagaca acgtgccttt cttgcgcctt atacccaaga aaggatacaa
     1141 actacgatgg attcacaagt caaaaaactc caccaagcgc tattagacca gcgaaaccaa
     1201 gaacaaaaaa aggctttaat tcaacgatcg atggatattc ttgccccctt atgttgctta
     1261 gtactaacat tattagcctt ttggttggta tggggagaat aaacagacat ggcattctat
     1321 ttaccctatc tactcatttc tgccagtatt ttaggaatta tttggcttag ctataaggct
     1381 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttattggtc
     1441 ggggtatttg ccttatcaat cgttttaagt ggtttattcg gcgttttaga gggtaataaa
     1501 cgtgtttcaa gaagcatttt attaggggat atcactcaaa agtatgaagc agcatgtgat
     1561 aaaaagaaaa aagagcaagc attagcacaa aaaatggagg aatttaccgc ttgttatgag
     1621 gacatgaaca atatttttgt ggatcaagag aaacgtttaa cagataaaaa catgaaaaaa
     1681 ctcactcgtt tttatcaaaa cttaccagaa aaacagcaaa aagaggtaca agaaaaatat
     1741 gagcaagtaa aaaaagacgt gcaatatttt aaagatacgc agacagaaga agcttgttgt
     1801 aacttattta gcgatacaat ccctttttca accagtgaac aagaacgaaa agaacggcaa
     1861 caatcggtca catacgaacg ttatgaaaca ctgcttcagc aagcaacaaa catacaaaat
     1921 ccaacgaaaa aagaaacggc attaaattat ctagaaagtg tcaaggaatg gttggatcag
     1981 caacaacaaa attgaatttg attagcaatc atgttaaact gaac
//
LOCUS       DACDID010000248         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDID010000248 DACDID010000000
VERSION     DACDID010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.254"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV236_002684"
     CDS             <1..378
                     /locus_tag="IV236_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8772839.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV236_002685"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV236_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8772840.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2001
                     /locus_tag="IV236_002686"
     CDS             1859..>2001
                     /locus_tag="IV236_002686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8772841.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDA"
BASE COUNT          554 a          339 c          432 g          676 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt t
//
LOCUS       DACDID010000249         1992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDID010000249 DACDID010000000
VERSION     DACDID010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.255"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            140..1606
                     /locus_tag="IV236_002687"
     CDS             140..1606
                     /locus_tag="IV236_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8772842.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          627 a          335 c          404 g          626 t
ORIGIN      
        1 tttgatggaa aaaatatgaa aaaacgatgt gtattaaata aaagaataca cacataaaat
       61 aacactgtct tgtaagtttg ttaaaaattg aatatgatta catctggata acaaattttt
      121 atcataaagg agagaacgaa tgaacaacaa acaattacgc tggtttactg ttgcgttaat
      181 tgcatttaac atggtttggg gcatggggaa tgtagttaat aactatgctc agcaaggagt
      241 atcagttgtc acctcttggc tattgatttt ggccatttat tttattccat acgcattgat
      301 cgttggacaa ttaggatcag ccttcaagga tagtaaagga ggcgtcagtt catgggtcga
      361 aaatacgaca tctaataaac gactggcata ctatgccgct tggacttact gggtcgtcca
      421 tattccttat ttggcacaaa agcctcaagc aattctgatt gctggtggtt gggcagtcca
      481 aggaaatgga aatatcgtaa atacgatgtc agtacaaatg gtagcagtta tttcacttat
      541 tatatttctt gcctttttat atttatcaac aaaaggactt tctactttga aagtgattgg
      601 cggactggcc ggaacagcga tgttcgtcat gtcgatctta tttattcttt tagctgtaac
      661 ggcaccgttc atcaatccaa cgatggaatt tgccacacca catatgaatc aagtgaaaac
      721 atatattcca acatttgatt tcagttattt cacaaccgtt tcgatgctag tatttgcagt
      781 tggaggggcg gaaaaaatat ccccttatgt caactctaca agaaatcctg caaaggaatt
      841 tcctaaaggg atgatcttct tagcagcaat ggtagggatt tgtgctgtat taggctcagt
      901 cgcaatgggt atgctgtttg ctagtgataa tattcctaaa gacttgatgg caaatggggc
      961 atacagtgct tttcaaattt taggtaatta ctatggtatc ggtaactcat tgatgatcat
     1021 ttacgcattg acaaacatga ttggacaaat ctccgcatta gccttttcaa ttgatgcacc
     1081 actaaaaatc ttgttagcag atgcggatcc agaatttgta ccaaactggc tacgtaaacg
     1141 taatcgtaaa ggtacattgg tgaatggtta tcttctgaca ggtatactag taggaatcat
     1201 tattttgttg ccgatttttg gtatcaaaga tatgaatgaa ttagtaaaat ggttgacgaa
     1261 cttgaactca gttgtcatgc caatgcgtta cctatgggtg ttcttggcat atatgatgtt
     1321 gaataaagct tataagaaat tcaattctga atataaattt gttaaaaatc caaaaatcgg
     1381 ttttatcttt ggtgcttggt gctttgcctt cactgcgttt gcatgtatcc taggaatggt
     1441 gccaaaagta gattatactg ctgatcctaa agcatggtgg ttccaattat ttacgaatat
     1501 catcacacca atcgtcttga tcttattagg tatgatcttg ccagcaattg ctcgtcgaga
     1561 caaaaacaaa aaaatcactg aaccagaaac agtggttcag ccttaaaata gaaatacttg
     1621 ttacaagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat
     1681 tgcttctcaa atttttgtga agtttcggct tgtttttcga agaagctact tctggaacac
     1741 cgtgaatcag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
     1801 aatagcccct catatttttc gtttggctgg gcttattacc gcagatgcaa gctttggaac
     1861 accgtttaag gaagaggttg taaaatcagc gatttgacct gagtattaga gaaacaaacg
     1921 gaaaaatagc tcctcatatt ttgccgcttt ttgagctaat gctgaaggtc agctgattga
     1981 acaccattta tt
//
LOCUS       DACDID010000250         1983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDID010000250 DACDID010000000
VERSION     DACDID010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.256"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="IV236_002688"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="IV236_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8772843.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="IV236_002689"
     CDS             853..1041
                     /locus_tag="IV236_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8772844.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="IV236_002690"
     CDS             1054..1611
                     /locus_tag="IV236_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8772845.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="IV236_002691"
     CDS             1643..1885
                     /locus_tag="IV236_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8772846.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          305 c          406 g          548 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 att
//
LOCUS       DACDID010000251         1895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDID010000251 DACDID010000000
VERSION     DACDID010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.257"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            803..1276
                     /locus_tag="IV236_002692"
     CDS             803..1276
                     /locus_tag="IV236_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8772847.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1285..1512
                     /locus_tag="IV236_002693"
     CDS             1285..1512
                     /locus_tag="IV236_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772848.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          614 a          312 c          289 g          680 t
ORIGIN      
        1 attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc taggaaatat
       61 ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat ctctattctt
      121 tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg ccatctatct
      181 gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt tatttttcta
      241 cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca tctacttgcg
      301 cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg attttccttt
      361 tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca aaccattgaa
      421 taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga atcggttagc
      481 tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct cttcgctaga
      541 taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa actagcatta
      601 tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta caatcaaaaa
      661 aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac gatatatttt
      721 tattgttttt cgataaaaaa tagttgtttt acaataatta ttctgctata ttttagctat
      781 caaagataaa agagggataa aaatgaaatt taaaacattg ctattaaaat ttgtcgtttt
      841 aggtataagt gccgtattta ttttgttcgg cgtacttttt tatactcaga tcgctacaag
      901 cgaaaaagga ttcaattttg attggcagac cacattattt gtttctgtca tttactttgc
      961 ccttctccta gggatcgtta ttgcttattt cttattgaaa ctattgaaaa aaatcgaaga
     1021 taaccaagct ttttccactc aaagcttaaa catactaaaa aaaatcgaac gaaccatttt
     1081 gtttattggt ttcgcctttt ttggcttact tcctaaattt atgaaagtag cggatttaga
     1141 cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt ttgcagtata
     1201 cacattaaca agcgtgctgg aaaaactgct tgaacatgcg attttactta agacagatca
     1261 tgatttgaca gtgtgaggtg cgacatggca attatcgtta atttagatgt agtattagct
     1321 caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg gcattacgat ggcaaacatc
     1381 tctattctaa aaaatggaaa agcaaaagcc atccgttttt ccactctcga aaaaatctgc
     1441 caagttttgg agtgtcaacc aggtgatttg ttgacctata aaaatgactc gaatactata
     1501 ccagaagaat aaaataaagt agaacaaaca gtaaataaaa aataatctga atgatagatg
     1561 tgtgagactt ttagaagtta tttacgtcta ttattcagat tttttatttc gattgaatac
     1621 cgttttatct ataaataaga tatgtactga ctcatacaaa gggggccttt tcttctccat
     1681 gtactatcac ttttcctgat acaactggtt tcctgttacg ttgtagttgg atctgctttt
     1741 agtataatac tttattcatt ccaccagact gatagcagtt tgagcagtct aaataatcat
     1801 gtagtattac aggccattct gtttcatacc ttgaagtgct taaaacttca gctaatggcc
     1861 cattgtgtta actcttactc cattcttttt tagta
//
LOCUS       DACDID010000252         1857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDID010000252 DACDID010000000
VERSION     DACDID010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.258"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IV236_002694"
     CDS             complement(<1..425)
                     /locus_tag="IV236_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8772849.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IV236_002695"
     CDS             complement(534..1565)
                     /locus_tag="IV236_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8772850.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          573 a          312 c          321 g          651 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacatt
//
LOCUS       DACDID010000253         1848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDID010000253 DACDID010000000
VERSION     DACDID010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.259"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..593
                     /locus_tag="IV236_002696"
     CDS             72..593
                     /locus_tag="IV236_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8772851.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(1292..>1848)
                     /locus_tag="IV236_002697"
     CDS             complement(1292..>1848)
                     /locus_tag="IV236_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772852.1"
                     /translation="RNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITRG
                     EFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLDE
                     LEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGLE
                     DLYDRFGIRLISWEHDPPIENRFE"
BASE COUNT          598 a          369 c          293 g          588 t
ORIGIN      
        1 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
       61 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
      121 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
      181 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
      241 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
      301 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
      361 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
      421 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
      481 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
      541 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
      601 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
      661 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
      721 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
      781 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
      841 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
      901 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
      961 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
     1021 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
     1081 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
     1141 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
     1201 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
     1261 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
     1321 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
     1381 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
     1441 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
     1501 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
     1561 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
     1621 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
     1681 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
     1741 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
     1801 taacaacaat aatgattaat attttgtttc tatttgataa atttctca
//
LOCUS       DACDID010000254         1837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDID010000254 DACDID010000000
VERSION     DACDID010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.260"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IV236_002698"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IV236_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IV236_002699"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IV236_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8772853.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          551 a          348 c          357 g          581 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttc
//
LOCUS       DACDID010000255         1834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDID010000255 DACDID010000000
VERSION     DACDID010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.261"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            153..524
                     /locus_tag="IV236_002700"
     CDS             153..524
                     /locus_tag="IV236_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8772854.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(808..>1834)
                     /gene="xerS"
                     /locus_tag="IV236_002701"
     CDS             complement(808..>1834)
                     /gene="xerS"
                     /locus_tag="IV236_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8772855.1"
                     /translation="IKDFVQDREDKDQSPRTTLEYLKNYKLFYEWLLSESIVPDTSIT
                     SIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLSTSTINRNITALKVLF
                     KYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKKLFLDKDTQNYLDYIA
                     NEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININLDDLSLDKNNVVVTR
                     KGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERM
                     VAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSSTNTTTLYTHIDNAAT
                     IDALNAL"
BASE COUNT          586 a          290 c          312 g          646 t
ORIGIN      
        1 aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt
       61 ggacaaaaga agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac
      121 attttggttt tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca
      181 ctgcattaac ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac
      241 tctattataa gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc
      301 gtacaatcga actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag
      361 tttcaatagg aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata
      421 caatccgtca tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac
      481 acggattaag gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa
      541 ttaaagaaag gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt
      601 atctaaattc attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt
      661 ctaaaatcaa accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga
      721 tcacaccaaa agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc
      781 ttatttccgc ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc
      841 attgtctata tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc
      901 agttaatacc aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt
      961 gtgagggctg acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc
     1021 aataccggat atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt
     1081 ttctgttact ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc
     1141 gaatgctgct atattgacag catctctttt atttcctttt ctagttacta ccacattatt
     1201 cttatctaaa gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga
     1261 tcctaaaatc agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa
     1321 agctcgttta ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt
     1381 atccttatct agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt
     1441 atctgtgatg gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg
     1501 attattggaa cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt
     1561 gatagtagaa gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc
     1621 tttttttaca ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata
     1681 ggcagttaac tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag
     1741 ccattcataa aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc
     1801 tttatcctca cgatcttgaa caaaatcttt tata
//
LOCUS       DACDID010000256         1802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDID010000256 DACDID010000000
VERSION     DACDID010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.262"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            409..1227
                     /locus_tag="IV236_002702"
     CDS             409..1227
                     /locus_tag="IV236_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8772856.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1300..1659)
                     /locus_tag="IV236_002703"
     CDS             complement(1300..1659)
                     /locus_tag="IV236_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8772857.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          592 a          272 c          342 g          596 t
ORIGIN      
        1 gaatggttta ttacctgatt gaacatgaat aaaagtaata tttttatttt gatggcctat
       61 ttctaggtta tgaattatcc agatcaaatt tgtgtattac attcagtaat aaggtgctta
      121 tcttaggatc aacttatgat actagctgac caaggatgta ccagttatag aaaatcagtt
      181 aaagtaaaaa tgataaaaat cttatgtcaa cactgattta ggctgacctg tgggttagga
      241 gaaagacagg tcggtagctt ttaatacaaa aaatggcttg tatgtatcta gatagttaac
      301 tgaaattaga aatgttctta tttttaacgg agtaagaaaa ataggctgct ttaggtctga
      361 atactttttc aatataatga agatatcaaa ggcgacagga gtgtttttat gaataagaat
      421 cctcataatc taaagttggt agcagtagat atggatggaa catttgctcg tactgattac
      481 acatacgata tcccaaggtt caaggctgtt ttatcgcgaa tgaaagctgt tgattgtcaa
      541 tttgttgttg caagtggaaa tcaatactac caattgagat cgttatttcc aaattattat
      601 gatgaattaa gttttgttgc tgaaaatggt gcttttgtta aagataaaac agaggtacta
      661 ttttcagccg atatgcctag aaaagcgatc gaggtggtcc ttgatttatg taaagactat
      721 cctgaagtaa aaaatgtgat gtgtggtctg aatagtgcct actgtgaaag aggaaaggtg
      781 agtcaagcat tttttgatct aaccaatatc tattaccata gattagaatg ggtagctgat
      841 tttgcagaag ttcaagataa aattttaaaa tttgctccga ctatgcctgt tgaaaaaacc
      901 gaattttacg ttgaattgtt ccgtgaaaaa ttagccgggc tattagtacc taccagtagt
      961 ggtcatggtt ctgtagactt gattctttct gagtgccata aaactttcgg cttgaaaatg
     1021 ttagttgagc gcttggggat taatcctgac cagtgcgttg cttttggaga tgggggcaat
     1081 gacattgaga tgttggagta ttgtggtctg agttatgaaa tggataatgc aacggaagct
     1141 gttaagcaag ttgctaagca tcaatgtcct tcaaatgacg aagacggtgt tttagtgacg
     1201 ctcgatcgat tgttccctaa cacgtaaaca ctaggtaatt catcaaaaaa cctcgatata
     1261 agaaatattc gaggttttaa aactatattt agaacagatt tacgaagcgt ctgatgagtc
     1321 aaaatagact tggacattgc tatctaaaat accactttca ttctttaaac gaattgtttg
     1381 atttgatgga gttacctcta atactaaatg cccattaata aaggtagtat cgtattcaga
     1441 tgttttaact gttgcttctt cgcaaggtat tgttaaatca tatgctggaa tataatgttg
     1501 tgtggaaact aataaaaaat gatcaccaat catacagcat ccctctgctg aagaaaattg
     1561 attagagcca tcatttaaag ttaacaagaa tccatccttt aaacgaggta cttgcttaaa
     1621 tacttctggt tgtacttcta aaaataaatt atcgctcatt ttcacgacca ccatcgactt
     1681 ccacagttga aattgacatt ttatttaaca cacctattcc cccttaacta taactcaatt
     1741 ctatataaaa aatattctat tctcaaataa ttgaactttt caaatcgcca atttacttcg
     1801 gc
//
LOCUS       DACDID010000257         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDID010000257 DACDID010000000
VERSION     DACDID010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.263"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..317
                     /locus_tag="IV236_002704"
                     /pseudo
     CDS             1..317
                     /locus_tag="IV236_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            472..1047
                     /locus_tag="IV236_002705"
     CDS             472..1047
                     /locus_tag="IV236_002705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8772858.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1090..1287)
                     /locus_tag="IV236_002706"
     CDS             complement(1090..1287)
                     /locus_tag="IV236_002706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772859.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1274..1456
                     /locus_tag="IV236_002707"
                     /pseudo
     CDS             1274..1456
                     /locus_tag="IV236_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          642 a          268 c          328 g          561 t
ORIGIN      
        1 ggggaacttc atctccagat tccgcgcgac cgcaatggcg agttcaagca acagactgtt
       61 cctgcttata gacggacgaa tgacacatta gaggagaccg tcattcacct cttccgaaaa
      121 ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca tcactacacg
      181 ccccaaacca tgtccaacat gactaaagtg ctgactgaag aagtaaatgc atttaaagtt
      241 agaactctaa atgataagta tgtcgctatt ttcatgattc cttcagtttt tcagatgata
      301 taccactcaa aaaacaatat gagagatgtt caggaatacc atctctaatg gcttataaat
      361 tggttttagg gatagtagat gttatagaca tatcaaggaa taaacgagca gctgcataaa
      421 agcagatgga attaaggaat aattgaattg taaaagaagg gaataaattt aatggcacaa
      481 ttattttaca gatatggaac aatgaatagt ggaaagagta ttgagctact gaaagtggct
      541 cataattatg aagaacaagg gaagagagtt atcataatga caagcgcaat agatactcga
      601 tcaggatttg gaaaagttga gagtcgtata ggtgtttcga aaccagctat tgcaattact
      661 tctgaaatgg atgtgtacgg agaattagtg gagattattg atcagtacgg aaaagttgat
      721 tgtattttgg tcgatgagtc tcaattttta agtagagaga atattattga tctatcactt
      781 atcgtaatga atgaggaagt accagtaatt tgctatggat tgaagaatga ttttcaaaat
      841 aatctcttca agggatccga agcattattg acatttgctg ataaaattga ggaaattaaa
      901 actatttgcc atttttgcaa taaaaaggct gtaatgaatg ctcgtattgt tgatggtatt
      961 ccagtttatt caggtgaaca gattgtagta ggaggtaatg aagattacct ttcggtatgt
     1021 cgcaaccact actacaacga agattgaaat aaaaaacgtg taatcaaata attgattaca
     1081 cgttttttat tattctgtta aaggttgagg atcagaaatt tttaggtatc tataatcctc
     1141 acctaaaaat ccgttaaaac gaagtaaaac atcatgacca tttaaatcag ttggaactaa
     1201 aattggaatt aatttatcaa cagtagtttt gttgtaaagt tcaaaagtgt tctccattcc
     1261 ttctggttct acaatcgaat ctttcaataa acaaatcaag aaatacagcc gcagaaaaga
     1321 gcagtttcaa aatgaagaat cactagagcg tttcctagta tccatctttg atacatacaa
     1381 tcaaaaattc ttaaatagaa gccataaagg cttccaacaa gtgacggata cattagcttc
     1441 aatgtttact gagtaaccca ttattttgca gaaggacgag ttatttacac aaaattattg
     1501 acgctccctg tttggagtgg taaaaagatt gagaaattgt ttcattagct attacgatta
     1561 ttattgtaat atatttttat tgaaaatcaa aattaaaata attcactgta aaaacaggat
     1621 tattttttct attttggtaa gtcaacagga acatagtttt caattgattc aattgactca
     1681 ttttcaataa tctgatccat aaatccaggg caaatattac cagacaaatt ataaaacctg
     1741 ctttcttctt cagtcactaa atttttaaat tctttttcag ttataatagc tccagtttt
//
LOCUS       DACDID010000258         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDID010000258 DACDID010000000
VERSION     DACDID010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.264"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IV236_002708"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IV236_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8772860.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DACDID010000259         1779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDID010000259 DACDID010000000
VERSION     DACDID010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.265"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            77..643
                     /locus_tag="IV236_002709"
     CDS             77..643
                     /locus_tag="IV236_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8772861.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            788..875
                     /locus_tag="IV236_002710"
     tRNA            788..875
                     /locus_tag="IV236_002710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:820..822,aa:Ser,seq:cga)
     gene            1209..>1779
                     /locus_tag="IV236_002711"
     CDS             1209..>1779
                     /locus_tag="IV236_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8772862.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          613 a          274 c          348 g          544 t
ORIGIN      
        1 aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag
       61 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
      121 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
      181 gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa
      241 tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac
      301 tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag
      361 tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc
      421 atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga
      481 atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat
      541 gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat
      601 tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa
      661 tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga
      721 tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag
      781 tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta
      841 ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa
      901 gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt
      961 cgtttgattg ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag
     1021 gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt
     1081 tttgattccc cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt
     1141 tttttgataa aaaaaatact agatgtaaga taaaagtgta aaaacgattt gttttaggag
     1201 gggaaacgat gtgtaccgca atcacttatg caacgaaaga ccattatttt ggaaggaatt
     1261 ttgattatga gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg
     1321 attttagaaa ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg
     1381 tatccaacta tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac
     1441 tgaattttcc tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac
     1501 catttgagtt tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat
     1561 tattggctac tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt
     1621 tacactggtt attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac
     1681 ttcatttata tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat
     1741 tatttaatct gaacaattat cgctcgctat caaatggaa
//
LOCUS       DACDID010000260         1777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDID010000260 DACDID010000000
VERSION     DACDID010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.266"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..500)
                     /locus_tag="IV236_002712"
     CDS             complement(54..500)
                     /locus_tag="IV236_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8772863.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(736..1662)
                     /gene="cysK"
                     /locus_tag="IV236_002713"
     CDS             complement(736..1662)
                     /gene="cysK"
                     /locus_tag="IV236_002713"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8772864.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          498 a          399 c          269 g          611 t
ORIGIN      
        1 gtttttttta cacagctagc tttttttagg tataacactt gtagaaactg tttttacatt
       61 tgcgcctttt ttgctgactc tttacaatcc ggacagagtc catacaattc caaccgatgt
      121 tcattaatct caaaatcagt cagtctgctt gcagccattt ctatatcttc aagccctggg
      181 taatgaaaat caacaatctt accgcagttt tcacaaatta catgataatg acgctttgaa
      241 ctgaaatcaa atcggctaga agcatcgccg tacttcattt cttgaacaaa tccaatcgaa
      301 gtaaataagc gaaggttatt gtaaacagtc gcaacactca tatttggaaa ccgatgttca
      361 agtgctttat aaatctcatc agccgttgga tgggaatgat gttcaatcaa atattctaaa
      421 accgcgtacc gttgaggtgt gattcgaatg ttctcttttt ttaacttttc gataacctct
      481 tcgactaact tattttccat gtaaacaccc cacgttgtta ttctcgtctt atcatactat
      541 ttttacattt taaaatccac ttaacatgaa aagaaaattc aattttattg tattttaatg
      601 aaaaaagacg taaaacagtc gcgttcacta ttctacgtct ctattcaaat aatttttctc
      661 atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa cctatctgga
      721 gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac gttcaccatt
      781 gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga cttttagcgc
      841 ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc gcccaacttc
      901 tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat acacagaagt
      961 atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag gtcccgcttc
     1021 tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga ttttcggata
     1081 cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga tacctgcaac
     1141 aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc
     1201 atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt
     1261 tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag gagtcaaaat
     1321 caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg tctcaggcat
     1381 cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga ttcctgtatt
     1441 tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt gttctgcttt
     1501 ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa aaaactcaag
     1561 ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga cgattggcgt
     1621 tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt cctccttgtc
     1681 tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga ttcgtctttg
     1741 actagcaaaa aaaagacaaa gatataccat atatggt
//
LOCUS       DACDID010000261         1748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDID010000261 DACDID010000000
VERSION     DACDID010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.268"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..182)
                     /locus_tag="IV236_002714"
     CDS             complement(<1..182)
                     /locus_tag="IV236_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8772865.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IV236_002715"
     CDS             517..951
                     /locus_tag="IV236_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8772866.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IV236_002716"
     CDS             974..1294
                     /locus_tag="IV236_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772867.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IV236_002717"
     CDS             1435..1626
                     /locus_tag="IV236_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772868.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          666 a          261 c          364 g          457 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattact
//
LOCUS       DACDID010000262         1748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDID010000262 DACDID010000000
VERSION     DACDID010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.269"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..1107
                     /locus_tag="IV236_002718"
     CDS             67..1107
                     /locus_tag="IV236_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8772869.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          684 a          247 c          289 g          528 t
ORIGIN      
        1 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
       61 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      121 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      181 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      241 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      301 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      361 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      421 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      481 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      541 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa
      601 tcagagggta tgaaaatcat accagaactt aataataata tattagacac taatagacac
      661 aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca
      721 attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa
      781 acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag
      841 aaaaaagctg aaatagaaag tggtatttcg atagtttttg aagaactcga tagttattat
      901 gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc
      961 gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa
     1021 aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgaaag ctccccaagc
     1081 actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt tgcttcatgg
     1141 agtaacatta aaattaaaaa ttttttatta ttctaattat attacgaata ttacaaaata
     1201 atagctattt taatagaatt tataagttaa aatattatga taaaaatcac aaacgtagcc
     1261 ttttacgtca taagtgttaa aagaaataat taatttcaaa tatagaaaag ggaacgtatg
     1321 tttgcatact cctttttttt gagttataat aatacgcgaa gagagataat ttcattattt
     1381 aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac
     1441 attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac
     1501 ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg
     1561 gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt
     1621 taacctttca gtgttacggt attacccgca atggatttag tataacagat attcaaaagt
     1681 gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg
     1741 cctaagca
//
LOCUS       DACDID010000263         1724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDID010000263 DACDID010000000
VERSION     DACDID010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.270"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..790
                     /locus_tag="IV236_002719"
     CDS             194..790
                     /locus_tag="IV236_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8772870.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(848..1330)
                     /locus_tag="IV236_002720"
     CDS             complement(848..1330)
                     /locus_tag="IV236_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772871.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1436..1633)
                     /locus_tag="IV236_002721"
     CDS             complement(1436..1633)
                     /locus_tag="IV236_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772872.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          580 a          350 c          264 g          530 t
ORIGIN      
        1 attcctaaaa cacaacaatg aaagcgataa ctaagaaaat ccttacataa cgatttttct
       61 tattaaaatt gttttttgaa cttcgcttat tttttgtgag catatctgtt tacttacttt
      121 ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa atgcaaaacg caaatcaaag
      181 gagaaatttt attatgacaa cttttactga cactttgaaa aaccgccgtt caatctatca
      241 tctaggacgt aatgtttcac tttctaatga agaattaaca actttgatca aagaagcaat
      301 taaagaaagc ccaactgctt tcaatgctca atcaacacgc gcagttatcc ttttcggaga
      361 tgcacatgaa aaactttggg aaatcactga agaagcactt cgcccattga ctccagcaga
      421 agctttccca aatacacaaa acaaattagc tggtttcaaa aatggttatg gtacagtatt
      481 attcttcaaa gatactgacg tggtaaaagg tctacaagaa caatttgaat tatatgcaga
      541 caacttccca gattggtcag aacaatcaaa cggaatcgca acagctaata cttgggtagc
      601 attagttgat aaaggattgg gcgcaaactt gcaacattac aacccagtta ttgacgaagc
      661 agttgcaaaa gaatggaata tcccttcaaa ctggaaacta cgttcgcaat tagtattcgg
      721 ttcaccagaa acaccggctg gggaaaaaga atacatgaac gatgccgatc gcttccgcgt
      781 atttggataa ttttatttga aataaaaaac agaggttgtg acaaacatct tgtcgcaacc
      841 tctgttttta ttctggatca atcataatat caatctctgc ctcatcgttt aagcgttggg
      901 acaaaaatgg aactaattct tctaatgatg cagaaaattt cagcatcggt cctcgccatc
      961 cgtttcggaa aaaattcatt tttacgattt tagaaccttt tggctgatat aatacaattt
     1021 tattgatctc agaaaaagga acaccttttt tgtcaaagct attcaaaaca agtccttctt
     1081 ctgtgattcc tctactgtcc aacaaaaaac taataaaaat cattgctgac aagatccctt
     1141 ttgccaattc atctgctgta ggatgactga tgagtgtata ccaaatgact agtgacacta
     1201 agaagggtat caccaagtag acccatcgat ttcgcacagt ccgataaata atttcttttc
     1261 gaaccagcca aagaaataaa aacatcacaa caacgaataa aaacaataga accgtaaaga
     1321 atgttaccat gacaactcgc ctcgcttttt cttctccaga aaataggaaa agaatactga
     1381 tttaccttct caaagaaatt ggacttgcag cacgccgcac gtccaattgt tttctttatt
     1441 caaatgtatc atattttgca acaaccatgc ttgctttttc atctaataat tcttggtcct
     1501 ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt catttttgat acaacttcac
     1561 caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata ttgttctcct aaattaatca
     1621 tttcttccgt catcaaaatc ctcctaaact gctggaactt ttctccacat taccactgct
     1681 atttctttgg agatttaact cattgatttt tatttacagt taaa
//
LOCUS       DACDID010000264         1708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDID010000264 DACDID010000000
VERSION     DACDID010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.271"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..832
                     /locus_tag="IV236_002722"
     CDS             50..832
                     /locus_tag="IV236_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8772873.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(958..1689)
                     /gene="erm(A)"
                     /locus_tag="IV236_002723"
     CDS             complement(958..1689)
                     /gene="erm(A)"
                     /locus_tag="IV236_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8772874.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          549 a          263 c          314 g          582 t
ORIGIN      
        1 agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca tgagcaattt
       61 gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa aagatttatt
      121 tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg gtttacgcat
      181 taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa
      241 aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg attctgttag
      301 accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat atcctccaaa
      361 aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac aaattcagga
      421 accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga atagtatttc
      481 tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag atattcgaag
      541 agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg agcgtaatgt
      601 aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta cctcgaaaga
      661 tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt tactggatat
      721 agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat attcaaaggt
      781 gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt aggctaattt
      841 tattgcaata acaggtgctt acttttaaaa ctactgattt attgataaat attgaacaat
      901 ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta ctttaattta
      961 gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata gtttattaat
     1021 attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga aaagaacacg
     1081 atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt ccttctttga
     1141 aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg gcttaggatg
     1201 aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca ttaatagtaa
     1261 acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa cgataagata
     1321 gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac tgatgttata
     1381 aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt ttagaatatc
     1441 cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca cttgacataa
     1501 gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct ctttggtaaa
     1561 atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat tcgtgtgatt
     1621 caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt tagggttttt
     1681 ctggttcatt ataaccttct ccttactg
//
LOCUS       DACDID010000265         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDID010000265 DACDID010000000
VERSION     DACDID010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.272"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1700
                     /locus_tag="IV236_002724"
     CDS             <1..>1700
                     /locus_tag="IV236_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8772875.1"
                     /translation="TTTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEE
                     IKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLG
                     FQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFE
                     ADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPD
                     WLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDT
                     SWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRN
                     LEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPS
                     YQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYND
                     DMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDID
                     S"
BASE COUNT          617 a          295 c          355 g          433 t
ORIGIN      
        1 accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc ttccgggaac
       61 gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag tgaaaccctg
      121 ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc tgaaaatatg
      301 gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg tacaccgatt
      361 tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca tgatcagtat
      421 ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc tgtcttagga
      481 tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa aatctatcgg
      541 aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa tcgtatgatt
      601 gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt tgaagcagat
      661 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta tacaaaattt
      721 gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc cattgacatg
      781 gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg gttaacaaaa
      841 gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga ttttccacga
      901 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
      961 atgcaaaaaa ttattgagga atataatcag tattatgata cttcctggtc attagcagac
     1021 attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc ggaattcaaa
     1081 caatttggga aacaaatcga tttagcgatt gtagtggatc gtctattaac aggttttgac
     1141 gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg attgatccaa
     1201 gctttttcac gtacgaatcg tacgtatcct gataagacaa aaggattgat tgtgacgttt
     1261 cgtaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta ttcaaaagca
     1321 aaagaagaat caggtttgat ttatccaagt tatcaggaat ccaaaaaaag attcaaacaa
     1381 gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca tacgccactt
     1441 gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgctta tgaatcgtta
     1501 gtgacttatg acgattacaa tgatgacatg gtgaaatctc caactttgaa caatcaaatt
     1561 caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt aatagaggaa
     1621 gaaccagaaa aagaaggaga agatttttca acgatcgaat tttatagtga aaataccact
     1681 aagttgtatg atattgattc
//
LOCUS       DACDID010000266         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDID010000266 DACDID010000000
VERSION     DACDID010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.273"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1700
                     /locus_tag="IV236_002725"
     CDS             <1..>1700
                     /locus_tag="IV236_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8772876.1"
                     /translation="TTTEFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEE
                     IKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLG
                     FQVEHENTVEPTSLENKIYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFE
                     AEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESD
                     WLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDT
                     AWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRN
                     LEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPS
                     YQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYND
                     DMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDID
                     S"
BASE COUNT          603 a          298 c          368 g          431 t
ORIGIN      
        1 accaccacag agtttacgaa atttgcttcg gagtttaata ctggggtttc ttctggtaat
       61 gccaaagcga atagcttgat cgtgggaaca ggaagtgcca aagagttaag tgaaactctg
      121 ttggcagatg ccaatgcgaa tgttgtgatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca ggaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 gggcaacata tcgtgtttgt agttgatgag tgtcatcgag cgttaagtgc tgaaaatatg
      301 gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg tacaccgatt
      361 ttccctgaga atcggaaaca agccaaaggg caattggcac gtacgaccca tgatcagtat
      421 ggagaggtct tgcacaccta taccatcaag aatgcattag aagatggctc ggttttagga
      481 tttcaagtag agcatgaaaa caccgtggag ccgacttcat tagagaataa aatctatcgg
      541 aaattaaaag aagtagaaac atatgcagag tattcaccgg aacagatcaa tcgtatgatc
      601 gatcaaatgg agtctgtcaa aaaagaaagt tatttggacc ctactgtttt tgaagcagag
      661 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag acaatgccta tacaaaattt
      721 gattttcgaa atggccgtcc aacgaagtct gcgatcttaa ccacgagttc cattgatatg
      781 gccaaaaagt attatcgggc catcaaagag atgacgaaag aatctgattg gttaataaaa
      841 gagtttccca atcagcctat ccgtgaagga cgaacgatgg aggatcctga tttcccacga
      901 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
      961 atgcaaaaaa ttattgagga atataatcag tattatgata ctgcctggtc actagcagac
     1021 attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc ggaattcaaa
     1081 caatttggga aacaaatcga tttagtaatc gtagtggatc gtctattgac aggttttgac
     1141 gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg attgatccaa
     1201 gccttttcac gtacgaaccg tacgtatcct gaaaaaacaa aaggactaat tgttactttc
     1261 cgaaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta ttcagaagct
     1321 aaagaagaat cgggtttgat ttatccaagt taccaagaat ccaaaaaaag attcaagcaa
     1381 gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca tacgccactt
     1441 gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgcgta tgaatcgtta
     1501 gtgacttatg atgattacaa tgatgacatg gtgcaatctt caactttgaa caatcaaatt
     1561 caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt gatggaggaa
     1621 gaaccagaaa aagagggaga agatttttca acgatcgaat tttatagtga aaataccact
     1681 aagttgtatg atattgattc
//
LOCUS       DACDID010000267         1685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDID010000267 DACDID010000000
VERSION     DACDID010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.274"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..750
                     /locus_tag="IV236_002726"
     CDS             <1..750
                     /locus_tag="IV236_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8772877.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          581 a          232 c          354 g          518 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggata
//
LOCUS       DACDID010000268         1680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDID010000268 DACDID010000000
VERSION     DACDID010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.275"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="IV236_002727"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="IV236_002727"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8772878.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          515 a          348 c          365 g          452 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
//
LOCUS       DACDID010000269         1669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDID010000269 DACDID010000000
VERSION     DACDID010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.276"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..556
                     /locus_tag="IV236_002728"
     CDS             200..556
                     /locus_tag="IV236_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8772879.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            566..1465
                     /locus_tag="IV236_002729"
     CDS             566..1465
                     /locus_tag="IV236_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8772880.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          562 a          292 c          343 g          472 t
ORIGIN      
        1 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
       61 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
      121 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
      181 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
      241 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
      301 tgcagaaagc caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa
      361 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
      421 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
      481 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
      541 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
      601 ggacatcaat aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
      661 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
      721 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
      781 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
      841 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
      901 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
      961 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
     1021 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
     1081 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa
     1141 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
     1201 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
     1261 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
     1321 tgtcttgaaa gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg
     1381 tgcccatcac ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca
     1441 agcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
     1501 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
     1561 gcggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
     1621 atgcaagcgt gtattctttt tttgaacgat aatcacaaaa aaaaatata
//
LOCUS       DACDID010000270         1669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDID010000270 DACDID010000000
VERSION     DACDID010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.277"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /locus_tag="IV236_002730"
     CDS             <1..776
                     /locus_tag="IV236_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8772881.1"
                     /translation="YDYSQNQIINQSKSPYSNCIWVCWWQGLDQSPEIVKACVKSIKR
                     NAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWLDA
                     TFYCTKPIPDEYFDIDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFL
                     NYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTFDKIPSNNPLCDELIKNLNNIYDEKV
                     WNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGMLVENKLL"
     gene            798..>1669
                     /locus_tag="IV236_002731"
     CDS             798..>1669
                     /locus_tag="IV236_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8772882.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIK"
BASE COUNT          661 a          172 c          325 g          511 t
ORIGIN      
        1 catatgatta tagtcaaaat caaataatta atcaatcaaa atcaccatat tctaactgca
       61 tttgggtttg ttggtggcag ggattggatc aatcgccgga aattgtaaaa gcttgtgtaa
      121 aatcaataaa acgaaatgca gggaaacata ctgttataat tttaacagaa gataattata
      181 gacaatatgt tgatatacca aaatgggtgg aagaaaagaa aaacaaagga atcattacga
      241 gaacaaatta ttctgattta ttaagattat ctttgctagc aaaacatgga ggtttgtggt
      301 tagacgccac attttattgt acaaaaccaa taccagatga gtattttgat atcgatattt
      361 ggtctattag gaggcctgat tatgctcacg catccgtagc atcaggatat tttgcgggtt
      421 attcattagc atgtaatcaa gagtctagat ggatgtttgc tataattaga gacttttttt
      481 taaattattg gaaaaataat gatatgatgg tggattattt gatgatagat tatatgattg
      541 ttttagcaca gagaataaat catgaaatag cacagacatt tgataaaata cctagtaaca
      601 atccattatg tgacgaattg attaaaaatt taaataatat atatgatgaa aaagtatgga
      661 acttattaaa gaaagatact ttactgttta agttgacatg gaaacaatct tttaaaaagc
      721 aaaaagatga taaagttact ttttatggga tgttggtaga aaacaaatta ttataaatat
      781 tagaggtata aaaagttatg aaacacaagg taagtgtgat agtaccaatt tacaatgttg
      841 aaaaatattt aagaaaatgt gtggatagtt tattgagtca gacattaaaa gagatagaaa
      901 tcgtattagt tgatgatggt tcgccagatg cttcggggga aatagcagat gagtatcaaa
      961 aaaaatattc taatgtaaaa acaatacata gagaaaatgg ggggttagga ccggcaagaa
     1021 atacaggaat tgagaatgcg acaggagaat atgtggcttt tcttgattca gatgattggg
     1081 tagaaagtga tatgtatgaa aaattatact tagttgcgtc caaagaaaat gctgatatag
     1141 tagttagtgg acattgtgat tttgcgaatg acaaagctat ggtgaaaaag caacatcctt
     1201 tgagtgggaa tgtatatgat acgcctaata gtattaaaaa tgtacgaaaa aatttatttg
     1261 gacatagtcc aaaagatcaa gaagtagagg ccttcccgat gtcagtatgt atgtctttgt
     1321 ataataaaaa tctgctagat aaatatagac taagatttga aaaaatttta tctgaagata
     1381 caatttttaa cttgaatgca tatgatgtcg caaataaaat tgcttttaca gaatatacag
     1441 attattgtta tagaaaagaa gaacaagagt ctattacaaa aagtttatct ccaaataagc
     1501 agacggaatt taaagaattt ttaataactc taaaagctat agcatcgaaa gaagatttag
     1561 aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg taggctttat gtgggcttag
     1621 ttgatgatgc aaatttggga attaaagaga aaagaaagta tataaaaaa
//
LOCUS       DACDID010000271         1654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDID010000271 DACDID010000000
VERSION     DACDID010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.278"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IV236_002732"
     CDS             <1..153
                     /locus_tag="IV236_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8772883.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IV236_002733"
     CDS             159..575
                     /locus_tag="IV236_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772884.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IV236_002734"
     CDS             681..944
                     /locus_tag="IV236_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8772885.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IV236_002735"
     CDS             944..1216
                     /locus_tag="IV236_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8772886.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IV236_002736"
     CDS             1293..1622
                     /locus_tag="IV236_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8772887.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          630 a          208 c          326 g          490 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatt
//
LOCUS       DACDID010000272         1619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDID010000272 DACDID010000000
VERSION     DACDID010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.279"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..1411
                     /locus_tag="IV236_002737"
     CDS             23..1411
                     /locus_tag="IV236_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8772888.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
     gene            1418..>1619
                     /locus_tag="IV236_002738"
     CDS             1418..>1619
                     /locus_tag="IV236_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8772889.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIE"
BASE COUNT          610 a          224 c          331 g          454 t
ORIGIN      
        1 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
       61 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
      121 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
      181 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
      241 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
      301 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
      361 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
      421 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
      481 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
      541 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
      601 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
      661 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
      721 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
      781 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
      841 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
      901 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
      961 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     1021 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     1081 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     1141 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     1201 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     1261 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     1321 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     1381 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     1441 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     1501 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     1561 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaat
//
LOCUS       DACDID010000273         1589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDID010000273 DACDID010000000
VERSION     DACDID010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.280"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(188..1519)
                     /locus_tag="IV236_002739"
     CDS             complement(188..1519)
                     /locus_tag="IV236_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8772890.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          548 a          286 c          233 g          522 t
ORIGIN      
        1 ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa aataatgatt
       61 atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa atatgatcct
      121 tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt tgttatctca
      181 tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca attcgtaaaa
      241 acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca gctgctcgcc
      301 agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa ggaatttgca
      361 ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc aaagaaaaaa
      421 tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc aatccaaaaa
      481 taataggttc gcttatattg aatatagcgg gagttaccga taatttagca aagtccttat
      541 aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta tttcccgatc
      601 cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga atttgctctt
      661 tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga tctaacaatg
      721 aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca atagatgtaa
      781 ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta actatcgtgg
      841 taattagctt atttatatcc atattaaaca attgaatgat tataaatgac aataaagcaa
      901 acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa acagcgggag
      961 gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta ataaataatt
     1021 ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct gtataggata
     1081 taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta aacatcatct
     1141 gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca atatgatttg
     1201 tataatttct gcttgcacac atatgataag atactgctgc tacaactaat aatgtagtta
     1261 tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc aatgtactag
     1321 tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta ataagtgtca
     1381 caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc aaagttctta
     1441 aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat acaatgtcct
     1501 tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga ttactttttc
     1561 cacccaaata atacatcact tatcaaaaa
//
LOCUS       DACDID010000274         1580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDID010000274 DACDID010000000
VERSION     DACDID010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.281"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1475
                     /locus_tag="IV236_002740"
     CDS             <1..1475
                     /locus_tag="IV236_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8772891.1"
                     /translation="LRVQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAI
                     PKAGGMALFFPFISVMLLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLR
                     ENSEEFQETIELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFS
                     YVSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQR
                     IRAMKETSIQEGSDSPLKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASG
                     SGKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENI
                     RIGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFL
                     KNAPILILDEITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQG
                     KLVDQGTHNELYRRSEIYKNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT          544 a          231 c          297 g          508 t
ORIGIN      
        1 atttaagagt gcaaattgca gacgatttgt caaatttacc attatcctat ttttcaaaac
       61 ataatttatc agatttatct caaactatca tgtctgacgt tgaaggtatt gagcatgcga
      121 tgagtcatgc aatacctaaa gccggcggta tggctctgtt tttccctttt atttcagtga
      181 tgcttttggt tggtaatgtc aaaatgggat tagctgttat tttgccaacg ttatttagtt
      241 ttgtcttaat cttgttatca aagaaatccc aaacgaaagc caatactaaa tattacgata
      301 ctttgagaga aaactcggaa gaatttcaag aaactataga attgcagcaa gagattaata
      361 gctttaatct atctaaaaaa gttcaagaca gacttttcaa aaaaatggaa gagagtgaaa
      421 ggattcattt aaaggtagaa ttaagtactt tttcagtcat ggccttatcc tctattttct
      481 catatgttag tttagcagta gtcattctag taggtgttca cttactgtta acgggtgaag
      541 tgactatact ctacgtcgtt ggttacttac tagccgcaat aaaaataaag gattccttcg
      601 attctatgaa agaagcagtg cttgaaatat tttatttagc tcctaaaata cagcgaatta
      661 gagctatgaa ggaaaccagt atccaagagg gatcagatag tcctctgaaa tcttttgaca
      721 ttgagttgcg agatgtctct ttctcatatg ataacaatac cccaatttta gatcatatct
      781 cttttacggc taaacaagga gaagtgactg cgttagtagg tgctagtggt tccgggaaaa
      841 ctagtatctt gaaattagtg tctagactgt atgattatga tgaaggctgt attttaattg
      901 atgggtatga tattaaacgt gtatctccag cttctttgtt tagcaaaatc gctattgttt
      961 ttcaagaggt aactttgttt aatacaagta ttttggaaaa tatacgtatt ggaaatagtc
     1021 aagcgtcaga tgaggaagtg aaaaaagcag ctcgtttagc taactgcgaa gactttattg
     1081 aaaaactacc tgacggttat catacattgg tcggagaaaa cggaagttca ctttctggag
     1141 gagagcgaca acgcctatcc atagcacggg cttttttgaa aaatgcgcca attttaattt
     1201 tagatgaaat tactgccagt ttagatgcgg aaaatgaaaa acgtattcag gaaagtttaa
     1261 atcgtttaat acaagataag acagtactaa ttatatcgca tcgcttgaag tctattgaaa
     1321 aggtaaataa aattgtagtg atggatcaag gaaaactagt tgatcaaggt actcataatg
     1381 agttgtatcg aaggtcagaa atatacaaaa atctcataaa gaaaactaaa ctatctgaga
     1441 aattcgtcta tgaaaaagaa gctcaatcac gatgatgaaa taggctttaa attaacctac
     1501 aatttttggg atatagttat aattcttttt caaaaagaat aattttatta aacagcatct
     1561 aattttgaat acacgaaaga
//
LOCUS       DACDID010000275         1568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDID010000275 DACDID010000000
VERSION     DACDID010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.282"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            146..658
                     /locus_tag="IV236_002741"
     CDS             146..658
                     /locus_tag="IV236_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772892.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            929..1552
                     /locus_tag="IV236_002742"
     CDS             929..1552
                     /locus_tag="IV236_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772893.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          616 a          193 c          263 g          496 t
ORIGIN      
        1 taacactttt aatagccata aaccgatgag aggtacttat ataaaatttc tcgaaaatta
       61 taatgattta aaatctacca ataattatca atttggaaac ttgtcttatg agatgataag
      121 taaagttcta agtttgtgat gataaatgaa attaaatagt ataaatgatt tatttgctat
      181 tataggtgtt attgctaaca tacttttttc gtgtttgaat tttagattta ctcaaaagaa
      241 caaaaagaac aaactcagta tagaacaaat gaaaaaagac attgaaactg atttaaaaaa
      301 tgaccataaa gaaagaagaa aaattgaaga atatacttct ttaatggaaa agtgtaaagc
      361 aactgatgca atttttagct ttttaagaaa aagtgatgag ataatcacac gtgaaaatat
      421 agatttagaa aatgagatta gtaaacttga atctatcttt tccgatataa attctcttaa
      481 gtatttaatc gtcgaagaaa attcggatag ctttttagaa tttgaaaaat atctaacaga
      541 tcttatagat aatagaaata atgaattaat gagacaaaac attgcaagat tccttactaa
      601 attagtttct gaatggaaaa gtattatttc tcaaaatatg gataaattaa gatattaagg
      661 atgttagttt taaaataaac taatgtcctt atttttgtgt tcacatttct gttacctaag
      721 aaatacttaa aggaggtgat gtagccagca acgactacaa cttgaacccg ctatctacag
      781 tttatttgtt gtttcttttg ggtatactct aatggtacaa atccatattt gcttaaagcg
      841 ttgatttttc agcggttttt ctttattgca aagtgtaccc taagacctgt atactaatag
      901 taaatagatt ggataggaat gatggcaaat gtatttagct tatttaagag tttcttccga
      961 cgatcaaagt ttagctcggc aacataagct aattgaaaat tggtcagtca aaaataatat
     1021 tgctgaagac gatttaaaaa ttttcgagga aaaagtctct ggaaaaaata ttgaaaacag
     1081 accagagctg caagagctta tttctttcat acgtgaacga gatacgatta ttattcctag
     1141 tcttgatcga ttagcaagaa atagtaaaga tacaaaagac ttacttcaat tgtttcgtag
     1201 taaaggtgct tctattgaaa tattagacct accgtcgttt aatggggtaa ctgaccctgc
     1261 attaagagac ttgctaacaa atttagtcat agaagtgttt agctatgttg ctgaaaacga
     1321 acggaaaaaa atcaaagagc gtcaaaaaca aggcattgaa attgcaaaga aaaatggtcg
     1381 ttatactggt aaaaagattc aataccatgc caatgcaaca ggaaaaaaca aattgattta
     1441 cgatgaaatt gttgatggtc tgaagaaaaa agaaactatt tctaaaatag caaagaacgc
     1501 aggagttaca agaaaaaccg tttatcgagt aaaaagagat ttaggaatgt aatatgttcc
     1561 agttagaa
//
LOCUS       DACDID010000276         1566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDID010000276 DACDID010000000
VERSION     DACDID010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.283"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..380)
                     /locus_tag="IV236_002743"
     CDS             complement(3..380)
                     /locus_tag="IV236_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772894.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(680..1561)
                     /gene="galU"
                     /locus_tag="IV236_002744"
     CDS             complement(680..1561)
                     /gene="galU"
                     /locus_tag="IV236_002744"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8772895.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          429 a          353 c          239 g          545 t
ORIGIN      
        1 tattaccgtc ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta
       61 gttgtatgtc taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg
      121 attagataca gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc
      181 aattcaacca tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac
      241 gtttcttttt ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac
      301 acaaatatcc aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca
      361 acagcgacta attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct
      421 ttttattcta ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt
      481 ttatacaact taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa
      541 agtctttttc tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac
      601 gacagttgcg caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca
      661 gcaggccact tatttccagt taactttctg ttcttagctt tttactcaac tggataatat
      721 atgcacttaa tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca
      781 tatatcctaa cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg
      841 cctctgtctg gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg
      901 gttcttgagt ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg
      961 ctaaattact tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac
     1021 gacccttttc gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg
     1081 gtacacgcat gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt
     1141 gagtcaatgg gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat
     1201 ttcctataaa tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac
     1261 ggatgaagtg aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt
     1321 ccttttcccg caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc
     1381 ttttttcttt tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca
     1441 caataaactg gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg
     1501 ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca
     1561 cttgct
//
LOCUS       DACDID010000277         1558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDID010000277 DACDID010000000
VERSION     DACDID010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.284"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..797
                     /locus_tag="IV236_002745"
     CDS             138..797
                     /locus_tag="IV236_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8772896.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            816..>1558
                     /locus_tag="IV236_002746"
     CDS             816..>1558
                     /locus_tag="IV236_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8772897.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAG"
BASE COUNT          598 a          188 c          303 g          469 t
ORIGIN      
        1 agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat gttattttta
       61 taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt cttaaaaaaa
      121 agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta aataacaaaa
      181 gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt tattatatac
      241 tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt agtaacccag
      301 atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc
      361 aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg
      421 tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac
      481 tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt
      541 tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg
      601 atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga
      661 ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt
      721 cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta
      781 ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt
      841 gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa
      901 aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa
      961 ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga
     1021 taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata
     1081 atatagagga tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc
     1141 ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact
     1201 tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa
     1261 taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga
     1321 tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata
     1381 tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac
     1441 ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa
     1501 gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat aatgcagg
//
LOCUS       DACDID010000278         1554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDID010000278 DACDID010000000
VERSION     DACDID010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.285"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            310..1440
                     /locus_tag="IV236_002747"
     CDS             310..1440
                     /locus_tag="IV236_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8772898.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          435 a          256 c          339 g          524 t
ORIGIN      
        1 cttgttaggt gaggctccta tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc
       61 aatgggtaaa acagaaactg ttggattaag gctttttcca atagctaaga atgttttctt
      121 tacgttgtat agtgctaaaa ctcggacgat gagatgatgc taggtcagaa ttcttgttcg
      181 aaaaggtttc tgaccttttc tttatatttt tgtgactgac ttatttgatg agacaaactc
      241 gctcctcacc tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat
      301 tggtggattt tgggtcctta tttgactttg tccatgatcg gtcttttaga agtatacagt
      361 gccagttctt atcgtttatt gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa
      421 ctaattttca tatttttgag ttggagcgtg atcttcttag ctcgttcagt caaactacac
      481 tatttacttc accctaaaat agcaggatac ggtttagcct tatcgatttt ctttttagta
      541 ttagtaagaa tagggatatt cggtgtcact gtcaacggcg cacaacgttg gatctctctg
      601 tttggcattc aattccagcc ttctgaactg gcaaatcttt ttttgatttt ttatttaagc
      661 tggttttttc gtgacggaaa tagtagccca aaagatctaa aaaaaccatt cctgattaca
      721 gtaggtataa cttttctgat tttatttcag ccaaagattg ctggagcatt gatgatcctt
      781 tcgattgcgt gggtcatatt ttgggcagcg gcggttccat ttaaaaaagg gatctatcta
      841 atcgttactt tttctgcatt gctgattgga gcagcaggcg gggtattata tttaggaaat
      901 aaaggttggc ttccacagat gtttaatcat gcttatgaaa ggatcgcgac tttaagagat
      961 tcttttatcg atagtcatgg tgcgggctat caaatgaccc attcgtttta tgcactatat
     1021 aatggtggca tatttgggcg gggattagga aatagtatca caaaaaaagg gtatcttcca
     1081 gagacagaaa cagattttat tttttcgatt attacagaag agcttgggct aatcggtgca
     1141 ttgtgtgtgc ttttcttatt gttctcatta tgcatgagaa tcttttgttt aagcagtcgc
     1201 tgtaaaaatc agcaagctgg attgtttctt ttaggattcg gaactttgct attcgttcag
     1261 acgataatga acgtgggaag tatcgcgggg ctgatgccaa tgactggtgt gccattgcca
     1321 tttgttagct acggaggaac aagttatctg atcctttctt taggaatcgg aatcacgttg
     1381 aatatttctt caaagataca ggccgaagag ttggcgctct atcgacctga aaaacaataa
     1441 agagaaactg agaaactctc agtttgaatt atcataaagt acaatctttt atttttcaag
     1501 cgagaggttc ttttagaaag aggaattcgc aatgaaagta tctcaaaaaa tcat
//
LOCUS       DACDID010000279         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDID010000279 DACDID010000000
VERSION     DACDID010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.286"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..573
                     /locus_tag="IV236_002748"
     CDS             <1..573
                     /locus_tag="IV236_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8772899.1"
                     /translation="EWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFSKEDFHEL
                     LDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQFTFKPEI
                     KKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEK
                     EKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            594..1127
                     /locus_tag="IV236_002749"
     CDS             594..1127
                     /locus_tag="IV236_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8772900.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
BASE COUNT          648 a          262 c          253 g          361 t
ORIGIN      
        1 gagtgggttc gctattcact ccaacaattt aacgaattac aaagtagcta tgcaaagact
       61 atgtttcgat tactaaaaca atatcgaact aaaggttttg cttatttttc taaagaggat
      121 tttcatgaac tattagatat tccaaaaagt tactggaaca accaagcaaa cgttgataat
      181 cgtgttataa agcctatacg acaggaatta accgcaatct tcaaaggatt atcaatcaaa
      241 aagaaatacg gaaaagggcg aggaaaacct gtcgttggct atcaatttac gtttaaacca
      301 gaaatcaaaa aagcagatga ttttaataaa catgcaccaa ccaaagaaga acaaaataaa
      361 gcattggctt tagctgataa acaaaaagaa tacgaagaac ctgctacaac aattaacgaa
      421 aaaatcaaac agaagaaacg agaacttgaa ctcctccaac aaatcaaaca actagaaaaa
      481 gagaaagaag ctcttgaaaa acaaacagag tccgcacttt cgaaaaaaga gaaaaaaagt
      541 ttattatctg atttagataa tttattccag tagcaaccta ggaggcaacc agtatgcaac
      601 cacaagaaac ctttacaata aaggaattgt cagaactttt taagatgagt aggcaagcaa
      661 tatcgaaaca tatccaaaaa ttagattctt ccatgatagc taaaaatgag cgtggttata
      721 aggttgtatt gcgttctgga gtattgcaac ttgctcgcaa cttaggcaac caaatgttgc
      781 tagataggtt gcacgagtac gaacaagaca acgataaaaa tgttaatata ccaagcaaaa
      841 acgtggttac aaacaagttg ctagatcagt tgcgtgaaga aaatgaattt ttacgagaac
      901 aacttgccct cagagagcaa aataattctc atatacaaaa actactcgac cagcaacaac
      961 aactcaccct acaagccaac aaacaaatcg aaaaactgca agaaaaacta cagttgactt
     1021 atacagaaga agctccagag aatgactcaa cgactctttc tgaagcaaca gaaacaatag
     1081 acaaacaaat tgatactgaa aaaaaatggt ggcaactttg gaaataactg gagggaacat
     1141 tcatgacaag aaaaaatctt ctattcggtt ctattttact aggaattcta ggattcagtc
     1201 cgatgttatt gtcagtgatc aataaaggga ataaacatgg tgaattgaaa ttgaatccaa
     1261 attattacaa cagtcctaac gaagaattgt ttgtaaaaga aacagataaa taataaaaaa
     1321 gactgctctt tcgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcaacg
     1381 cgcttatgaa gcgctaccaa aaatttatca tattttatta aaaataacaa cccagtaaaa
     1441 aatgactggt aaaaattcac aaagaaacgg aaagaagcaa ccgcagaaaa tcaagcttaa
     1501 aaagagcttt tgacctagac caat
//
LOCUS       DACDID010000280         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDID010000280 DACDID010000000
VERSION     DACDID010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.287"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IV236_002750"
     CDS             101..868
                     /locus_tag="IV236_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8772901.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IV236_002751"
     CDS             1060..1458
                     /locus_tag="IV236_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8772902.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DACDID010000281         1497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDID010000281 DACDID010000000
VERSION     DACDID010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.289"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1453
                     /locus_tag="IV236_002752"
     CDS             <1..1453
                     /locus_tag="IV236_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8772903.1"
                     /translation="DKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGG
                     LGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPF
                     GVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPL
                     IVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIR
                     NLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKP
                     RVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFI
                     LTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGS
                     FFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTE
                     SAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          406 a          287 c          316 g          488 t
ORIGIN      
        1 agacaagggc ttcttcgggc agccgaaggg attgtcaact ttgttcttca ctgagatgtg
       61 ggaacgattt agttattacg gtatgcgtgc attacttatc tattatatgt acgatacagt
      121 tgccaacggg ggacttggat taccacgagc aacagcatta gctatcatgt cgatctacgg
      181 atctttgatc tacatgtcta gtatcgtcgg cggatgggta tctgaccgag tgttaggagc
      241 aaaacgcact gtattcttcg gtggagtttt cattatgctt ggccacatcg tcttggcttt
      301 accatttggt gtaagtacat tgtttgtttc gatggcattg attattatcg gaaccgcctt
      361 tttgaaacca aatgtttctg gcatggtcgg acatctttat tcaaagactg acttgcgaag
      421 agatgcagga ttttcgattt tttacatggg tgtcaactta ggggcattat tagcaccact
      481 aatcgttggt acaatcggtc aagaatataa ctatcattta gggttctctt tagctgctat
      541 tgggatgttc tttggattat tacaatacat catccaagga cgcaagacat tagacggagt
      601 gggtacaaca ccgccgaatc ctttgacaac tgaagaacgt aaaggattca tccgtaactt
      661 gatcatcgca cttgttgtta tcgctgcagt attcggcggg gcacaactaa ctggtcatct
      721 gacaattgac ttttttgtca atacaatcag tgtattgggg attatcttac cgttgtatta
      781 ctttatcaaa atgctgactt caaaagatgt aactgctgaa gaaaaaccaa gagtatgggc
      841 ttatatccca ttattccttg cagcaatcat tttctggtca ttacaagaac aaggttcatc
      901 tatcttagct ttatttgcta gtgaacgtac acaatctacg ctatttggtg taacgatcca
      961 agcaagctgg tatcaatcat tgaatccaat tttcatcttt attctgacac cagtattcgt
     1021 aacgatgtgg acgaaacttg gtgaacgtca accatctaca gtagtgaaat tctctcttgg
     1081 acttttattt gcaggattat cgtacttgct attgatgttg ccgggtatgc tttatggtac
     1141 aagtggaaaa gtaagtccat tgtggttgtt gggaagtttc ttcatcgtgg ttattgctga
     1201 attgtgtctt tcaccagttg gtttgtctgt gacaacaaaa ttagcaccac gagcatttga
     1261 atcacaaaca atcgctattt ggttcttagc tgatgcttct tcacaagcaa tcaatgcaca
     1321 aattgcacgc ttctacacac cgggaacaga atctgcttac ttcggtatta tcggtgcagt
     1381 agcaatcgtc ggtggtatct tgctatttac tgtgaaagaa ccaatcaaaa aactaatggg
     1441 ctctattcat taatcaatga acataaaaac aggaattgct tatttgcagc agttcct
//
LOCUS       DACDID010000282         1490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDID010000282 DACDID010000000
VERSION     DACDID010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.290"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1440
                     /locus_tag="IV236_002753"
     CDS             <1..1440
                     /locus_tag="IV236_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8772904.1"
                     /translation="EKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAAT
                     IIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRF
                     HQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQ
                     KVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDET
                     SYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYS
                     GYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSP
                     EKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLEL
                     TNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDK
                     IPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          497 a          267 c          315 g          411 t
ORIGIN      
        1 gaaaaggtca attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag
       61 gaaacgaatg ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa
      121 gcagctgcca ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac
      181 aaaatcaaac acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt
      241 tgtgaacatt gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt
      301 cgtttccatc aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa
      361 agctgcgaaa aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt
      421 tctaatagct taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag
      481 aaagttcctg cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg
      541 agagataata tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga
      601 gaacgattaa gagtctatct taatcaagaa gagattctac acggggatga aacaagttat
      661 cgtgtgattg aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac
      721 agtcagcatc ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa
      781 acttttctca aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc
      841 gtggaaagtg tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca
      901 attccaaaag gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat
      961 aaatggtttg tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt
     1021 cggcaacaag aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat
     1081 cctgttgcaa aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt
     1141 tttgaaccta tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat
     1201 atcaaagagc tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca
     1261 cgaacttcag gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct
     1321 gtaaagtatc tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa
     1381 actttagatc aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa
     1441 aaacgccaaa cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DACDID010000283         1490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDID010000283 DACDID010000000
VERSION     DACDID010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.291"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IV236_002754"
     CDS             2..400
                     /locus_tag="IV236_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8772905.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IV236_002755"
     CDS             384..1079
                     /locus_tag="IV236_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8772906.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          563 a          214 c          302 g          411 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
//
LOCUS       DACDID010000284         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDID010000284 DACDID010000000
VERSION     DACDID010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.292"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV236_002756"
     CDS             <1..1350
                     /locus_tag="IV236_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8772907.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          572 a          225 c          314 g          340 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata t
//
LOCUS       DACDID010000285         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDID010000285 DACDID010000000
VERSION     DACDID010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.293"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IV236_002757"
     CDS             105..1406
                     /locus_tag="IV236_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8772908.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACDID010000286         1440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDID010000286 DACDID010000000
VERSION     DACDID010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.294"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1363
                     /locus_tag="IV236_002758"
     CDS             <1..1363
                     /locus_tag="IV236_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772909.1"
                     /translation="RVKGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWIS
                     NNLIVSYILYVWLLNVCTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSA
                     IAEAIALTVIPIVMYYAYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVL
                     GGFVVSETRKKESIRLIVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQ
                     ALNVSDSLIGAMNNDLTTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLST
                     KFFPWSLLQNTPIQMIQFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLL
                     LLAVSAATKANYEHKVVTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQN
                     HEVRIGKEWHTMPYQVMDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRT
                     SVD"
BASE COUNT          447 a          237 c          268 g          488 t
ORIGIN      
        1 tagagtcaaa gggttatctt caattttttc tggtccaatc aattttacga cgtttaatca
       61 ttatgggagc ggagtgaatt atttttatcc ctacttgacc ttttttccgg cagtcatttt
      121 ttattggata agtaataatt taatcgttag ttatatctta tatgtttggc tattgaatgt
      181 ttgtactatc atgctgatgt ttcattatgg actaaaattt ttaaaaagaa tcgatgcggc
      241 ttttatattt agctgtttat atacatttta tgggtaccgt acgattgata tttatcatcg
      301 ttctgctatt gctgaagcga ttgcacttac ggttataccg atagttatgt attatgctta
      361 cgcactgatt tatgagaaaa agccatgtgc aattccatta gcagtcagta tgagcttact
      421 catttataca catgttcttt ccacttttat gagtgtaata gccgttggct tactagtctt
      481 aggcggcttt gtagtaagtg aaacaagaaa aaaagaaagc atccggttga ttgtggagtt
      541 gtcaaaggca gtaggaatga cgattctttt aacagcatac ttttggtatc cgatgttccg
      601 acaaatcatg gttcaatcga ttaatgagcc agataaaacg accttacaac accaagcatt
      661 aaatgtctcg gatagtttga ttggtgcgat gaataatgac ctgacaacct ttacggtagg
      721 aatcattggt attatttctt tgatctgtcc actcttcttt tttaaaggat ttaatcgcaa
      781 agaaaaagtg atttacctta gtgcggtagt ctcttggctc ttatccacca aattttttcc
      841 atggtcttta ttacaaaata cacccattca aatgattcaa tttccttggc gaatcttagg
      901 ctttcaagta ctgtttggct cgttgatttt atcgttcgtt ttcatgaaga gcacatcagc
      961 taagcaaaga aaagtagtta atataacggc aattgtttta ttactgttgg ctgtatctgc
     1021 tgcaacaaaa gcaaactatg aacataaagt tgtcactcag agagatcact tggtaatgaa
     1081 tcaagaaaat cttattcgtt acacgacact ccttcccgga ggactttatg actatgcacc
     1141 cacggaagca ttacaataca aagaacattt gcagaatcat gaagtaagaa ttggcaagga
     1201 atggcatact atgccttatc aagtgatgga ttcgaaaata gtctatacag tacatgaatc
     1261 taaaggacaa aaaataacgc ttcctgtatt tgattattgg ggaacagtcg caaaagtaaa
     1321 tggacaaaaa agagagattg ataataggac tagtgtcgat tgaaggaaca caaggagaaa
     1381 caatcattga agtttcttcc atttatcctc caactatgaa gttagctttt ttaattagtt
//
LOCUS       DACDID010000287         1434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDID010000287 DACDID010000000
VERSION     DACDID010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.295"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..1397
                     /locus_tag="IV236_002759"
     CDS             3..1397
                     /locus_tag="IV236_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8772910.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT          484 a          264 c          224 g          462 t
ORIGIN      
        1 aaatgcctat taattcttat gacaattacc cgttaacttg gcgaccagac aaacaaaaat
       61 tgaagcgtcc ttattatcga tcgttgattc aacaattaga acaagatatt ttaaagaata
      121 aattacagaa aaacactcag ttaccctccc aaagagagct agctgattat ttagatttaa
      181 attttactac cgttggcaag gcttataaat atggaattga gaaaggtttg ctgtatacga
      241 atattggaag tggcacattt atttctccca atgtctttga gtccatcacc atctctacca
      301 atgaagtaac cgaacatatc attgattttg gtctggttag ttcttttgaa gaatgtaata
      361 aattcgttgt cccgtttatt caatcaacta gtaataaccc aaatcttgtt ggtttgctta
      421 attaccaaga tcccaaaggc acaaattttc aattgactac tgcagcaaat tggttaaaaa
      481 ctcaaggagt cattccgtcc ttagaatctc tatctattgt ttccggtgtt caaaatggat
      541 tggctatcac actttctgca cttttctctc caggtgatag aatagcaaca gaccgatata
      601 catacgctaa tttcattgaa ttggctcagt tatatcattt agagatcgtt ccaattgatt
      661 ttgacgagca aggaatgcga cctgatatat tgattcagga atgtagaaaa aagaagattc
      721 atggtgtctt tcttatgcct tcttgtaata atcctatagg gtttcaaatc tctcacaaaa
      781 gacgagagca attagccgaa gtgattgaaa aagaaggtct atgggttatt gaagatgata
      841 tccattcatt tttaacaact tactatcaac atacgatcat tcctcctttc caaaatttat
      901 tacctaacca aacaatctac ttagctggaa tgacaaaatt tatttgttct ggattgcgag
      961 ttgcttatct tgtagttcca gtacatgtcc aaagcaagat agaacaagca atttttaata
     1021 taaatgtgaa aacttctgga ttagatgtgg aagtcatcac tcaaatcctc cattcaaatg
     1081 aagcaaatag gatcttagaa actaaattaa gtcttacaaa aagagccaat cagctttttg
     1141 attctctttt caaagtagaa aaaccaacga atcgtgtccc ttactatcga attcttccag
     1201 ttgatcctga gattgaccct aagataattg agaatgactt tcttgctaac ggtatccgac
     1261 tttatcattc cagacgcttt actacacgtg cgcaaccaga tgcattcgtt cgaatttccc
     1321 tatcctctaa taaccttgaa attttagctt ctggactaag tatcgtaaaa gaaaaaataa
     1381 aaaaatatga attataacct attgataaat aaaggaatca aatcttattg attc
//
LOCUS       DACDID010000288         1432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDID010000288 DACDID010000000
VERSION     DACDID010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.296"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(128..418)
                     /locus_tag="IV236_002760"
     CDS             complement(128..418)
                     /locus_tag="IV236_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772911.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="IV236_002761"
     CDS             complement(536..796)
                     /locus_tag="IV236_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772912.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="IV236_002762"
     CDS             complement(933..1346)
                     /locus_tag="IV236_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8772913.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          528 a          236 c          212 g          456 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg tt
//
LOCUS       DACDID010000289         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDID010000289 DACDID010000000
VERSION     DACDID010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.297"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV236_002763"
     CDS             complement(150..1337)
                     /locus_tag="IV236_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8772914.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDID010000290         1404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDID010000290 DACDID010000000
VERSION     DACDID010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.298"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(94..435)
                     /locus_tag="IV236_002764"
     CDS             complement(94..435)
                     /locus_tag="IV236_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8772915.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(1054..1386)
                     /locus_tag="IV236_002765"
     CDS             complement(1054..1386)
                     /locus_tag="IV236_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8772916.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          405 a          316 c          182 g          501 t
ORIGIN      
        1 attccttact caatgcctaa ggtaatacta cctttttatt aatcatcgtg actccttcta
       61 agcctttatc tgtaaaattt attcctccag tttttattct tctttatccg ctacaaattc
      121 aatgatttca aagctgacaa tataatcact caaatatgac caagtatctt caacagataa
      181 ttcgtctcca ttatcattca taagcgtcat tttaaaccat tcattacctt catcatcttc
      241 aggtagcaag ccgaatccaa ttggattatc agtcaatgtt tgatcgtata aatcatcctc
      301 gtctaatgct aaaacatcta atcctttaca atttccgcct cttctaaacg tcacaaatcc
      361 tttgtaatcc caacgttgga aagaaacctt aatagtatgt gtgcccaatc gtatattcgg
      421 atcataattt ttcatttgtc attcctccat tacgtaaatt tcattttctc tatgttttat
      481 atcaattttt tcactttatt caggacttgt tcatatttta tcgtttttcg tttacttaaa
      541 aatacataaa aaatttcgac tcgtttatgg cgtccgaaac gagaaaccct agtagacagc
      601 ggtaaatgct cgattgatcc aataatcaac gtcctcgcaa gtatctacat gtactgcagc
      661 acacgacaca atttcttgtg cgtcctcccc attacgagaa gacctactcc ttattccatg
      721 agtttgcgtc aaagtctaga tcccttagta gctgctcatg attaatctag acgctattct
      781 aacttgtcta acttctaaat tccctcttgt ttactctgcg cttattattt acacaaattc
      841 atttcaccac tatccaaaca agcgcattta ctcgattgac caatttttta cgttaccaat
      901 cgttaaaacc taccccgtca ctccccgaag ggaattgttg atagatcaag tctactcctt
      961 atttttctag tgactctaag ttcctttaga agtctcgaca atattttcaa gcaagcgaaa
     1021 aacgataaaa tacaagttat gagaatgatc aattcagccg atcaaacgcc ccatcccttt
     1081 tgcggtacaa cgcaagactc cattttttct tcgaccaatc agtacaccgt tttggtcata
     1141 aacatgtttg attgttggat atagctcttt taaggggata ggatctttta aaattatttg
     1201 cttttgtttc ccaatgtaat aaatcatttc ctctgagact ttgtctttct ggattatata
     1261 tttcttacac tgcacaacgg ttttactttg gccagttgat agtttcttcg tgattttctc
     1321 tgtagcggta caccgtttac cgtagtgaca aatatctttg agactcaaaa ttcctgtata
     1381 tgacataaaa taaaccaccc aatt
//
LOCUS       DACDID010000291         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDID010000291 DACDID010000000
VERSION     DACDID010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.299"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..666
                     /locus_tag="IV236_002766"
     CDS             52..666
                     /locus_tag="IV236_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772917.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(831..>1395)
                     /locus_tag="IV236_002767"
     CDS             complement(831..>1395)
                     /locus_tag="IV236_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772918.1"
                     /translation="SNTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKL
                     LGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNF
                     FYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYK
                     ELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          461 a          244 c          222 g          468 t
ORIGIN      
        1 cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt tttgagagtt
       61 gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga agaaatgaaa
      121 aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat tactcatcga
      181 cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat tgtggaagct
      241 attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta cttgaagaag
      301 aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat tggcaatcct
      361 ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat cgctgaacaa
      421 gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa agcaaacggc
      481 gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca acgtcgcctt
      541 gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa gattgctaaa
      601 gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat ggtcaatgac
      661 aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac atataattac
      721 tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa caaaccattt
      781 tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg ttaatcccct
      841 actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc ttgtgattta
      901 gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat agctagttct
      961 tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt atttggttcc
     1021 gttggagtca attctataga ttcttcataa aaatctctat acataaagct atcattaatt
     1081 ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca ttcttctata
     1141 gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat gaaattttta
     1201 atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa catgctccct
     1261 aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa atcaggtttc
     1321 tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga aataaaccta
     1381 tctctagtat ttgat
//
LOCUS       DACDID010000292         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDID010000292 DACDID010000000
VERSION     DACDID010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.300"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IV236_002768"
     CDS             complement(<1..276)
                     /locus_tag="IV236_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772919.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSI"
     gene            401..1186
                     /locus_tag="IV236_002769"
     CDS             401..1186
                     /locus_tag="IV236_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8772920.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          520 a          230 c          211 g          432 t
ORIGIN      
        1 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt agg
//
LOCUS       DACDID010000293         1392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDID010000293 DACDID010000000
VERSION     DACDID010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.301"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..125)
                     /locus_tag="IV236_002770"
     tRNA            complement(41..125)
                     /locus_tag="IV236_002770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Leu,seq:caa)
     gene            complement(143..214)
                     /locus_tag="IV236_002771"
     tRNA            complement(143..214)
                     /locus_tag="IV236_002771"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Gln,seq:ttg)
     gene            complement(224..296)
                     /locus_tag="IV236_002772"
     tRNA            complement(224..296)
                     /locus_tag="IV236_002772"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:His,seq:gtg)
     gene            complement(358..442)
                     /locus_tag="IV236_002773"
     tRNA            complement(358..442)
                     /locus_tag="IV236_002773"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Leu,seq:caa)
     gene            complement(463..533)
                     /locus_tag="IV236_002774"
     tRNA            complement(463..533)
                     /locus_tag="IV236_002774"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Cys,seq:gca)
     gene            complement(579..650)
                     /locus_tag="IV236_002775"
     tRNA            complement(579..650)
                     /locus_tag="IV236_002775"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Gln,seq:ttg)
     gene            complement(662..734)
                     /locus_tag="IV236_002776"
     tRNA            complement(662..734)
                     /locus_tag="IV236_002776"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:His,seq:gtg)
     gene            complement(737..810)
                     /locus_tag="IV236_002777"
     tRNA            complement(737..810)
                     /locus_tag="IV236_002777"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Trp,seq:cca)
     gene            complement(817..897)
                     /locus_tag="IV236_002778"
     tRNA            complement(817..897)
                     /locus_tag="IV236_002778"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(861..863),aa:Tyr,seq:gta)
     gene            complement(899..974)
                     /locus_tag="IV236_002779"
     tRNA            complement(899..974)
                     /locus_tag="IV236_002779"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Phe,seq:gaa)
     gene            complement(1010..1082)
                     /locus_tag="IV236_002780"
     tRNA            complement(1010..1082)
                     /locus_tag="IV236_002780"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Asp,seq:gtc)
     gene            complement(1092..1164)
                     /locus_tag="IV236_002781"
     tRNA            complement(1092..1164)
                     /locus_tag="IV236_002781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Val,seq:tac)
     gene            complement(1180..1251)
                     /locus_tag="IV236_002782"
     tRNA            complement(1180..1251)
                     /locus_tag="IV236_002782"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Glu,seq:ttc)
     gene            complement(1266..1355)
                     /locus_tag="IV236_002783"
     tRNA            complement(1266..1355)
                     /locus_tag="IV236_002783"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Ser,seq:gga)
BASE COUNT          393 a          365 c          320 g          314 t
ORIGIN      
        1 atagcaactc tctataagaa agttgctata ttgataatta taccggcggc cggggtcgaa
       61 ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa ttccgccacg
      121 ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca tgagggagtc
      181 aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat gatgggaatc
      241 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatc
      301 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
      361 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      421 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      481 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      541 tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      601 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      661 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      721 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      781 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      841 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      901 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      961 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
     1021 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1081 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
     1141 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
     1201 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
     1261 tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc
     1321 caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt
     1381 tctcaaaatg ag
//
LOCUS       DACDID010000294         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDID010000294 DACDID010000000
VERSION     DACDID010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.302"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            192..1067
                     /locus_tag="IV236_002784"
     CDS             192..1067
                     /locus_tag="IV236_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772921.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1212..>1372)
                     /locus_tag="IV236_002785"
     CDS             complement(1212..>1372)
                     /locus_tag="IV236_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8772922.1"
                     /translation="LEWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPA
                     TKEICHDL"
BASE COUNT          487 a          256 c          230 g          399 t
ORIGIN      
        1 aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa
       61 gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa
      121 aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa
      181 aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt
      241 acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg
      301 caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca
      361 tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct
      421 tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt
      481 cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag
      541 aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt
      601 cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca
      661 ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc
      721 tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc
      781 tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt
      841 attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct
      901 cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa
      961 aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata
     1021 aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata
     1081 acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc
     1141 aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta
     1201 cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat
     1261 tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta
     1321 tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa tc
//
LOCUS       DACDID010000295         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDID010000295 DACDID010000000
VERSION     DACDID010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.303"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..>1372
                     /locus_tag="IV236_002786"
     CDS             122..>1372
                     /locus_tag="IV236_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8772923.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDID010000296         1363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDID010000296 DACDID010000000
VERSION     DACDID010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.304"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IV236_002787"
     CDS             188..391
                     /locus_tag="IV236_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772924.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IV236_002788"
     CDS             602..1186
                     /locus_tag="IV236_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8772925.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          482 a          193 c          259 g          429 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttatta tcaaatgttg taaggatttt attcatttgt taatattaaa
     1321 gaacggaact ccaaaaaagt ccgtactcta atttgcagtc tct
//
LOCUS       DACDID010000297         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDID010000297 DACDID010000000
VERSION     DACDID010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.305"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1357
                     /locus_tag="IV236_002789"
     CDS             <1..>1357
                     /locus_tag="IV236_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8772926.1"
                     /translation="EDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFKTRNYIIELN
                     KEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIE
                     KFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGF
                     SMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFS
                     DEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAH
                     FHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSF
                     TSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVSY
                     FEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKE
                     QI"
BASE COUNT          487 a          190 c          244 g          436 t
ORIGIN      
        1 gaagatagag aaaaactgct gctagctaga tatttagaat ttgagaaaaa gaattatgta
       61 gtgctgccta agttatatga aacattaggt ttgtctaaat ttaaaacaag aaattatatc
      121 atagaattaa ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa
      181 gaagcagaga tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc
      241 tactttcaac gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg
      301 atcgaaaaat ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga
      361 gaattagtct cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg
      421 gaagaaataa acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt
      481 tctatgccat ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa
      541 tatctgttca atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact
      601 attagtttgt tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa
      661 tactttaaga gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt
      721 catatcccta aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg
      781 gaagaagaat ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc
      841 cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa
      901 atctctgaag aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc
      961 aatcgaaaaa attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta
     1021 cagtttttct tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag
     1081 aataaacgct atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat
     1141 ctctttttat tggtcggtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt
     1201 attgattttt cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt
     1261 aaaaattaca atattgtgat tgagagtcga atcacttctc gtacgaatat ttatttatct
     1321 gattgtttac ttgaaaaatt taataaagaa cagataa
//
LOCUS       DACDID010000298         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDID010000298 DACDID010000000
VERSION     DACDID010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.306"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IV236_002790"
     rRNA            <1..>1355
                     /locus_tag="IV236_002790"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDID010000299         1351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDID010000299 DACDID010000000
VERSION     DACDID010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.307"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..1147
                     /locus_tag="IV236_002791"
     CDS             35..1147
                     /locus_tag="IV236_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8772927.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          473 a          252 c          220 g          406 t
ORIGIN      
        1 aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg tttagcctta gtgcgttctc
       61 tacaggaact attcttggat ggcaatttta caataatgaa cactctcaga tagttgccca
      121 tgctcaatca gctcaaaaaa agttgatact gactaccaat gaacaacagc tcgttgaaaa
      181 aatcgatcaa ctattaagaa aaaatgatta tattggctca atttacgtaa agaaaaataa
      241 tcgcatcatc atggaaaaag gctatggcta tgcgaataaa ctcacgcaac aaccaaatga
      301 tccttctctt tattatcaga ttggttcggt tcaaaaagca ttgactactc ttttgatttt
      361 aaaacaagtc gagcgtgggc acctttcttt agatactaag ctggctcaat tctatccaca
      421 aattgctggc agccagcaaa ttactatccg tgacttgctg tatatgcgct caggcttgca
      481 ccgaactgct tctccaacca taccgatgac tgatgaagaa gtcgttcaat ttgcgattca
      541 gcacttaaaa tttacagatt atcagacctc tcattatgat tcattgaact ttaccatatt
      601 gactggaatt ttgatcaaat taacaaatca accgtatgaa aaattattga ccgatgatat
      661 catcgaacct ttaggattac agcatacaaa attttatgac caagtcaaaa attcatccaa
      721 tcatgcacta tcctatcaaa tgtctaaaga ggatgattac tttaaaccac tgacagaatc
      781 tgaaacagag atccataacg aattagggac tggtaatatc agtatgtccg tctatgattt
      841 aaatacgctg tttacacaag ttttatctgg aaaactagtt tccaaagact tactcttctc
      901 attatggcgt gaaaatgcga atggacatcc atatagtggt ggggtatata gcggagataa
      961 ttatatcctg tcgcaaggaa atatcgactc ttttgataca tctgctatat ttagcaaaga
     1021 ttcacaggat gctatcatca tggaaagcaa tataaaagct gacaaaacta tcaaactgcc
     1081 tactgctgat ctaagaaacc aaatctatga cctgatggaa ggaactacta agttggcaaa
     1141 tagctgatat gatgaaaaga tagcaaatac tacttctttg aatagcattt gcttaaaaaa
     1201 agtactcgac taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat ttttgctaca
     1261 gactttaatt ccaaataaac agaacagaaa gaggtgttct tctttctgtt ctgtccaaat
     1321 tattctaaga tttctcttta ccaacattat t
//
LOCUS       DACDID010000300         1344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDID010000300 DACDID010000000
VERSION     DACDID010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.309"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..>1344
                     /locus_tag="IV236_002792"
     CDS             62..>1344
                     /locus_tag="IV236_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8772928.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          455 a          261 c          255 g          373 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcaccac aaaataaaca actgagagga cgatacgcta
       61 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
      121 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      361 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      481 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      541 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      601 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      721 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      781 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      961 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
     1021 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1081 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1141 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg
     1201 catcaccaac gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca
     1261 cataaagaca atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag
     1321 aatctttttg atcaatcaat taat
//
LOCUS       DACDID010000301         1335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDID010000301 DACDID010000000
VERSION     DACDID010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.310"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..225
                     /locus_tag="IV236_002793"
     CDS             <1..225
                     /locus_tag="IV236_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8772929.1"
                     /translation="EVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSE
                     EDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            230..1177
                     /locus_tag="IV236_002794"
     CDS             230..1177
                     /locus_tag="IV236_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8772930.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          444 a          186 c          281 g          424 t
ORIGIN      
        1 gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt
       61 aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat
      121 attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat
      181 tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta
      241 tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga
      301 acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac
      361 gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat
      421 gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc
      481 agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga
      541 attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt
      601 ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc
      661 ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga
      721 tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc
      781 tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga
      841 agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg tttatataga
      901 tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt
      961 caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat
     1021 tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc
     1081 tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag
     1141 tggaatgact aattatttga aaactgaaat caattaaaac atactaagat aagattagag
     1201 ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat
     1261 agaagggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat ttggacagaa
     1321 cagaaagaag aaaac
//
LOCUS       DACDID010000302         1326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDID010000302 DACDID010000000
VERSION     DACDID010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.311"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IV236_002795"
     CDS             complement(<1..625)
                     /locus_tag="IV236_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8772931.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIK"
     gene            976..1275
                     /locus_tag="IV236_002796"
     CDS             976..1275
                     /locus_tag="IV236_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8772932.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          482 a          190 c          214 g          440 t
ORIGIN      
        1 cttttatttg taaaaatttt ctagcaaaac caagtgcttg caactgatta agcgtacaat
       61 tttttagatg ttccgagcgt acttcaatca tttcaaagga gttgctactt aaatcgatat
      121 tttttaaatc agtatcaaaa aagctagcat tttctaaatc attagtagaa aacattattt
      181 tagtgaggga acagtctaca aaatctgcgc ttgataagac agagtgctca aaggtacttt
      241 cttttatgtt tgaaccagat aaatttacat agtccatttt acaattctcg aataacgtgt
      301 tcccaatata actttccatc aatttagtac ctgttaattt acaattgaca aatttcacgc
      361 gatgcaacat gctttttgtc aatatacagc ctgaaaaatc acagttttcg aatatacagt
      421 cggtaatctc cattctttga aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt
      481 tgtaaataat tggatggcag attatttggt tttcgatgac ctctttttgt gaaaggatat
      541 tatacagttc aaaatcttcg ttttcataga tatgaaaaaa ggatccttta ttcaagcttg
      601 cttcattaac tttaggaaaa atcatattta attacctcca ttttcataaa gtcatattaa
      661 caaaagctaa accaacttac attagcagaa aatgagatag gttttcgcct ttttcgataa
      721 cgatagtgtc gctatctctt gaaacgttct tagaattgcc ctgagagaca caaattaggc
      781 gtaattctaa taaaaatctt agaataaaaa tttttactga tttaatttag aatgttttta
      841 aaataaaaat cgttgccata acagtgtatt gaaaggtttt aacaatccag taatgattta
      901 aaaaaatcta gatgtagcgt atactttgtt tatagaatgt agtgggaaga aatatatttt
      961 tgggaggaaa tcgtgatgtc taaaagtgaa gaaatagaaa acatggtact aaaattatat
     1021 gatctcgttt taaatccaga gatcaaagaa aaggaacgta tacttttgat agacgcaaag
     1081 acaggcttag aaaaaggtca gtattatcct aaagtcataa ataatttgga acgtagttta
     1141 cgtccgttag caatcagggg agaattgtct aaaccagtat caccgtttta tatggagata
     1201 tcaacaatag gaaaatttga aaaagaatta ggtcgaggaa tggcttcggc accgattact
     1261 tttgggcatt tataaaaacg agttattcat ctcaatgaat taaaaagtat agtcaatctc
     1321 ataatg
//
LOCUS       DACDID010000303         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDID010000303 DACDID010000000
VERSION     DACDID010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.312"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..573
                     /locus_tag="IV236_002797"
     CDS             <1..573
                     /locus_tag="IV236_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772933.1"
                     /translation="LDIAKRNTAFLLYRVLLRAGLITFISIVGFFLFTVIVFIWRTTA
                     PPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRGNVTTQPRRSWSIKLLNNS
                     GELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQLTVRDRIAGFMHFSHYDLE
                     EARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            760..>1322
                     /locus_tag="IV236_002798"
     CDS             760..>1322
                     /locus_tag="IV236_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8772934.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          430 a          161 c          264 g          467 t
ORIGIN      
        1 ctagatatag caaagcgaaa tacagcattt ttattatatc gggttttact tcgagcaggt
       61 ctcataacat ttatatcgat cgttggattt tttctattca cagttattgt atttatttgg
      121 agaactacag caccaccatt gccagagatt acatatggag aatttccgtt tagtcttgaa
      181 tatgaaatag atgaggaaag aattttgatt gaagatacgg tgattgcaga atttatagga
      241 agttttagag ggaatgtgac aactcaacct agacgaagtt ggagtataaa acttttaaat
      301 aatagtggag agttgaaagc atttcattat gaattcttaa taatagaaag tgaagatgta
      361 acaatcactt taagtagtgg ctctgctgga tattttatga atgatggtgg aagcaagcca
      421 cagctcacag tccgtgatcg tattgcaggt tttatgcatt tttctcacta tgacttagaa
      481 gaagctcgta aaatacttgc agaatatggg atcacattaa tcaattggga atatgcgcca
      541 ccgataagaa attttttcaa tgattattat tagtcttata aaaaacagaa tgtaaaattt
      601 atgtgcgcga gtatttagtt ttccattttt tgtttgatag atatatagca ttgttgaagc
      661 atgggatagt ggtgccaatt ttatcaatga ggttggtcag gcagttaaca taattggtga
      721 tgctataaat ccatttaatt ggaattggag gaattaggtg tggaaacagt gagattatgg
      781 atattcatga aaaattttcg ttcggctttg agatatgtta ttctggaatc aaaatcaggt
      841 tattatctga ttgacacaga tcactatact ttttggggag gctacctttt tccgattttt
      901 aattggataa tcccccataa ggttatgaaa attaacttgc ctgcagaaga aataaataga
      961 ttaatactca acaaagattt acaaaggaaa agagagaaag aacattctaa tgttgcttgg
     1021 ataggtccag catttgcagt agtgacttct tcaattgtcg gtcctatgct agagagtatt
     1081 tatagtgatt tttctattgg aatacgttat ttatttgtta ctttaagtat agctgttctt
     1141 ttgtgcataa agggttatgt tagcaagaag aataaagata ttttaacact aatcggtgta
     1201 aacaatttat ctagtactaa aattatactg tttcctgaat ctataaaaga aggatcaagg
     1261 ttgttgataa tttttataat gcttatactg ttttctgttt tatttggggt gatgtttata
     1321 ga
//
LOCUS       DACDID010000304         1319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDID010000304 DACDID010000000
VERSION     DACDID010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.313"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(300..914)
                     /locus_tag="IV236_002799"
     CDS             complement(300..914)
                     /locus_tag="IV236_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8772935.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          423 a          261 c          221 g          414 t
ORIGIN      
        1 tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt
       61 tttatttttc tgacactaca tattcttata actcatttct cataactgtg aaacaactta
      121 taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata
      181 actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa
      241 acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac
      301 tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc
      361 aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc
      421 aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat
      481 cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg
      541 attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt
      601 ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt
      661 gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata
      721 cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg
      781 ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct
      841 tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa
      901 taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc
      961 ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct
     1021 gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa
     1081 acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt
     1141 acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg
     1201 tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta
     1261 ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaa
//
LOCUS       DACDID010000305         1308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDID010000305 DACDID010000000
VERSION     DACDID010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.314"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            26..>1308
                     /locus_tag="IV236_002800"
     CDS             26..>1308
                     /locus_tag="IV236_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8772936.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          409 a          285 c          261 g          353 t
ORIGIN      
        1 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
       61 tattctagac ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga
      121 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
      181 ccactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
      241 gaatgatgtc agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa
      301 acactgtgga aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc
      361 ccgcagagtc aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat
      421 tgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
      481 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
      541 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
      601 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
      661 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
      721 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
      781 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
      841 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
      901 attgaatggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga
      961 gatagtcgat cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca
     1021 ggtttttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     1081 agactacaag gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg
     1141 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
     1201 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg
     1261 agaacgaatc tttttatatc gaggaaaata tttcaagaaa actaagaa
//
LOCUS       DACDID010000306         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDID010000306 DACDID010000000
VERSION     DACDID010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.315"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..>1292)
                     /locus_tag="IV236_002801"
     CDS             complement(27..>1292)
                     /locus_tag="IV236_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8772937.1"
                     /translation="DQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLE
                     QLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGN
                     DWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITEL
                     MDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFS
                     TSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLC
                     FPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIH
                     LQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTT
                     HNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          418 a          179 c          233 g          462 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tc
//
LOCUS       DACDID010000307         1291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDID010000307 DACDID010000000
VERSION     DACDID010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.316"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(182..1207)
                     /locus_tag="IV236_002802"
     CDS             complement(182..1207)
                     /locus_tag="IV236_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8772938.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          371 a          220 c          257 g          443 t
ORIGIN      
        1 tcttccctgt ggaaaaaata gtttaacaca aacattatag ttatccacag ctatctaaaa
       61 cagatggaaa ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag ggatgagtga
      121 ttgaaaaaag aacagagtaa ctgacttatc ctcagtgttt ctgttctttt ataattctgt
      181 tctacatttc ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa agaatgattc
      241 ttttttcttc agggtattgg tggtggatta gttggaataa aagttctgtg gctttgattc
      301 ctaactgatt tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg gaggacaata
      361 agggatcact gaatcctgcg atcgaaagat catcaggtat ctgctttcca gattgttctg
      421 ccactttgta aatactcaat gattttaatg catcggtaga aaaaatcgca gttggtcttt
      481 ctcgtagctt cattaaatca ttcattgctg ccatgctatc ttctaagcga aagccgccgt
      541 ctataatcca ttcattacgg atatctaggt ggtgatcaaa taagcattga cggtaaccat
      601 tgagacgatc attggataca taaacttctg tcggagcatg gagacagccg atatgacggt
      661 gtccattctt gatcagtgtt tccaccaaat cgtaactatc ctggaaatta tccgtgtcca
      721 ctgagtagat gttcgtgtag tcgtttccga cttttccgat taccacaata ggaatattat
      781 attgatccaa ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata ataattcctc
      841 ggatcatttt ttcttggatc tttttcgcgc atttttctag ctcttcttct ggtgtttttg
      901 ccgtttgtag aatcaagtcg aagttatttt gttcagaaac ggcagaaatg gattgaataa
      961 catctgaaaa aaaaggattg ccataggtcg tatgagaagt acgatgagat aatgctagaa
     1021 tcgcgtcaaa accatttcct gacagtcctc ttgcaatttt gctcggttca taattcaatt
     1081 ctttgattgc ttgaagtaca cttgcttttg ctttatcaga gatatttggc tggttattta
     1141 atactctgga gacagttgat tttgagacat tagctttttt cgcaatgtca taaatagtag
     1201 gtttcaattt tatatgcacc tcctttttgg gagcgtttta gaataattcc atagtacaat
     1261 agacattctt ttttgtctat gattttgaaa a
//
LOCUS       DACDID010000308         1281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDID010000308 DACDID010000000
VERSION     DACDID010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.317"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..265
                     /locus_tag="IV236_002803"
     CDS             2..265
                     /locus_tag="IV236_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772939.1"
                     /translation="MQYLGSFDYTDENIINYVASLPPYQSVRLVDFASDELVLTTMGN
                     FLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            280..711
                     /locus_tag="IV236_002804"
     CDS             280..711
                     /locus_tag="IV236_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772940.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            893..1198
                     /locus_tag="IV236_002805"
     CDS             893..1198
                     /locus_tag="IV236_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772941.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT          476 a          178 c          268 g          359 t
ORIGIN      
        1 catgcaatat ttgggctcgt ttgattatac agatgaaaat attattaatt atgttgcaag
       61 tttacctcct tatcaaagcg ttcgcttggt agattttgca agtgatgaat tggtattaac
      121 aacgatgggc aactttttag attatgtcgg agacaaaaaa tggttggaaa aaattttgtc
      181 taaattgatt gcgaaacaaa caggagaaga acccataaat gcgattcaat tgttcaatcg
      241 atatgaacaa gaaaacaatg attaaagagg agagaaaaaa tgaaaagatt aaaacgaagt
      301 atttttattg tgctagcatt gattgatttt gttgggacag gttgtgaagc aattgacgtt
      361 caaaaagctc ccttatcgga aataaaacaa gcaataacaa aacaagatga agaacaaccg
      421 ctaaatacac aagtagaaat agtgaatcaa aataaaccga gtttttctga agaagatttt
      481 tcgttagaaa atggaaccag gcaatttttt tcagatttgg atcgtttaaa tcgggtaggt
      541 gtagcaagtg cacttttgca taaaagcagg atgccaacag aagaacgaga ccgattatac
      601 attaagccaa ctggctggaa acaaaagaaa atgaagaatg gcgattatct ttataatcgt
      661 tgtcatttga ttggctatca acgtgcgaca agtttcccca taagtatgta atacgaaacg
      721 ggaggattaa tcttaagatt aatcacaact aacctatttg acagttggaa tgaaagctat
      781 catgtctgct gaatcaactg gtctgatact cctacatgca ataaagtcta ctaggaagtt
      841 attgtatgaa gctaggtaaa ggcggcagaa caatttctaa gtgtggaatc atatgaagtt
      901 ggatatgccg ggacgctata aattactcag ggtgagactg tttttaatta aactgacgaa
      961 cttgcgaatg tacgggtcta taaccgactt atattcgaaa gatataagat tgcgaatgca
     1021 aagaacatta tcgaaaagta agaatgttgg tcatgaaatt cgagatggaa gactagcggt
     1081 gtatctgtca agagttggta aaacatgttt gattgattca gatgagagtg ggaatgcgac
     1141 aaaacgtttt acagaagaaa tagagcaact agcagaactg aatatacgat atttttaaat
     1201 actttagcgg agaataaaaa aatcctctac agaaaactac ggagggcaac atgtgctatt
     1261 gtttcaacaa tacattttta a
//
LOCUS       DACDID010000309         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDID010000309 DACDID010000000
VERSION     DACDID010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.318"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..1237
                     /locus_tag="IV236_002806"
     CDS             68..1237
                     /locus_tag="IV236_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8772942.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          468 a          192 c          217 g          402 t
ORIGIN      
        1 gaaaaaagga agtcccttct gtagaataga gttaccacaa cacattcaca gaaaagagga
       61 cttccatatg aacgatttta ctacagaaat tatggaaata ctaatcaata aaggtgattt
      121 ggatgatttg tttcgtcgtc acctagaatt ggctattaac gctttattac aagctgaatt
      181 aacggtattt cttgactatg aaaagtatga tagagctgga tttaattcag gtaattcccg
      241 taatgggaat tattcgcgct cttttaaaac agagtacggt gaattgaatt tagtaatacc
      301 tagagatagg aatggagact tttctcaaca aactttacct gcttataaaa gaaccaatga
      361 ctctttagaa accactatta ttcagctagt tcaaaaagga attactatgt ccgaaatctc
      421 tgatctaatc gaaaaaatgt atggtcatta ttacaaacca caaactattt caaatatgag
      481 taaaattgtg tctgaagatg tttcggcatt taaagaaaga actttagaag ctaaatactc
      541 agtcattttt atggatgcta ctcatattcc cttaaaaaga caaaccgtat caaaagaagc
      601 tgtctatata gtaattggta tcaggttgga tggaactaaa gaggttctag gatttactat
      661 agctccaact gaatctgctt atgtttggaa agagattctt caagatctaa aagaccgtgg
      721 tttagaagag gttttgttag tcgtaacaga tggtttaagt ggtattgacg atagtattca
      781 tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc catatatcta gaaatatcgc
      841 tcataaggtt cgtgttagtg atcgacaaga aatctgtagc gatttcaaat tagtttacca
      901 agcaagttca aaagaagaag caaataatca aataaggttt atgatagata aatggaaaaa
      961 gcagtatcca cgagtggtta aattgctcat gaatcctgct atattaacct tttataattt
     1021 ccctccgtca atcagaagaa ctatttattc aactaatttg attgaaggtt ttaacaaaca
     1081 attaaagaaa tataccaaaa gaaaagagca atttcctaat gaagaatctc tggagagatt
     1141 tctagtatct caattcaaca actataatca aaagtttcta tgccgagttc ataaaggttt
     1201 taaagaaata catgatacat tagaatcaat gttttaactt gatcaacaga atgggctttt
     1261 ccatttacac aaaattatt
//
LOCUS       DACDID010000310         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDID010000310 DACDID010000000
VERSION     DACDID010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.319"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..795
                     /locus_tag="IV236_002807"
     CDS             <1..795
                     /locus_tag="IV236_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8772943.1"
                     /translation="KKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRF
                     QVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQ
                     LVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDP
                     IDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSL
                     DLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            851..1069
                     /locus_tag="IV236_002808"
     CDS             851..1069
                     /locus_tag="IV236_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772944.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          474 a          160 c          241 g          401 t
ORIGIN      
        1 aaaaaaatag ttttatcttg tgccacttta ttaaaagagg caggtattcc taatattgct
       61 attgttacta atggatcatt agtaactcag gatgttgcaa aagaattatt caaagcaggt
      121 attaatcgat ttcaagtaag tttagatgga aaaaatgcta gtagccatga agcacttaga
      181 ttatatccaa acagttttga aaaggcaatt aatgctatca aattatttag agaattgtcc
      241 gaagaagtgg atgttgcttt ttgtcctact agttttaatt acaatcaatt acgagaagta
      301 tatcaactag taaatactct taaagtgaag tctttaagaa ttcaaccatt tatgcttagt
      361 ggtaggggaa tgcaaaatag ggaaatagtg ccaactgttt ctcaatatag aaaactagtt
      421 tctgatatta actatattaa aagaaatgaa gattatacca agatagattg gggagatcca
      481 attgatcata tcatccgaca cagaacttta ttgaaagatt tttcgaattt tgtttctatt
      541 aggtcagacg gaggattaat ggcatcacct tacttgccaa ttcaagttgg aaatattaaa
      601 tgtcacagtc tttctaaata ttggcgagca ggactttata aagttttttc tttagattta
      661 gttcaagata tagctactca agtttcttca atacaagata tgagtaaaga attaaaaaaa
      721 ggacatatta aatggcttaa tgaagatata gaaattgata ttctcgatga atacaaacat
      781 gatattttaa tttaggttta tcattggaaa gtttagataa acaaagtact tttagaaagg
      841 agggaaaaaa atgatttctg aacctcaaat gaataatatg tatgaaagcc caactataat
      901 gggagctggt gaagctgcgc caagagcttg ttcagcaatt atggtgtgtg tgttattagc
      961 atctgcagga gtaggtatcc aagcaggagc ggtgtatact tatgctgtag ttgctacagt
     1021 agctgtagct gcagccgcag tttggaaaac agtaggatta gttggttagg aattaatagg
     1081 agactaactg tctcctattg attccaatga ttaagctaag gaggaatttt aagaaaataa
     1141 tgaaatacaa taatttaaaa ccgaaattac ttaatgagaa catagaatat ataagagaag
     1201 aaaattatgg atatttaacc atagttccca aaaatggaac tgaattgaaa gataacgttt
     1261 tcaatccaac agtaag
//
LOCUS       DACDID010000311         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDID010000311 DACDID010000000
VERSION     DACDID010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.320"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IV236_002809"
     CDS             24..1103
                     /locus_tag="IV236_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8772945.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DACDID010000312         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.321, whole genome shotgun sequence.
ACCESSION   DACDID010000312 DACDID010000000
VERSION     DACDID010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.321"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            43..420
                     /locus_tag="IV236_002810"
     CDS             43..420
                     /locus_tag="IV236_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8772946.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            408..746
                     /locus_tag="IV236_002811"
                     /pseudo
     CDS             408..746
                     /locus_tag="IV236_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            880..>1264
                     /locus_tag="IV236_002812"
     CDS             880..>1264
                     /locus_tag="IV236_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8772947.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNR"
BASE COUNT          460 a          203 c          279 g          322 t
ORIGIN      
        1 acataagtaa agtggcaaga aattaagaga aagagtgatc agatgaatat caaacaagtc
       61 agtgaagaaa agggcatttc ggccgataca ttgcgctatt atgaacgaat cgggttgatt
      121 ccaccagtta atcggacaaa aggcggaatc cgtgattata cagaagaaga cctaaaatgg
      181 gtcgacttta cgatctgtat gcgaaatgcc ggattatcga tcgagtcttt gattgaatat
      241 attcgcctat ctagcgaagg agaagcaact gtatttgagc ggcgtaagct tttgattgaa
      301 gaaagtgaac ttttgacgaa aaaaattgct gaaatgcaag aatgtttgga acgtttacaa
      361 ggaaaaatca agaaatatga tcgtgtgtta tctagaaata aaatggaatg tatcaagtaa
      421 aaaaatcaag gagcatcgct atggagttac gcttactaaa atatttttgg acaattgcag
      481 aagaaggaac aatctctcaa gccgcagaag ttctacatct tacacaacca accttaagcc
      541 gacaactgaa ggagttagaa gagtaactag gaacagaact gtttgtacgc gaaaaccgca
      601 aaatgatctt gacagaagca ggttattttc taaaaaagcc gagcagaaga aattttagat
      661 ttaacgaatc aaaccactca agaattcgag aacagaaaaa aacaattatt cagcggccga
      721 tttacgatcg gctgtgtgga agctgatagt tcgaatacat tggcactatt actagaaaag
      781 ttgatccgtg attatcccag cgttcaattt catatccaca gcggaacaag tgatgatatc
      841 acggagtgac tagaaaaaag cctgttagat ttagcgattt tgctagatcc agtagataca
      901 gagaaatacg aaacgatttt gttaccaaga aaggaaaaat ggggattgct cattttagaa
      961 aattctgttt tagcaaaaaa agacgtgata acaaaagaag actttgcgaa tgtacctctt
     1021 ttgatctcaa agcgtccaga aatacagcga atgtttgctg actggttagg cataaaaaag
     1081 gagcagttgc tgatcacagg aacgtacaat ttgattttca atgtatttgc gctaattgag
     1141 aatcaggtag gtgctgcttt agtcatagaa ggtgctgtaa gtcatcattc aaagcgagtg
     1201 acattcgttc cgcttgaccc aatgattgaa acgaactgca tgctagtctg gaaaaagaat
     1261 cgga
//
LOCUS       DACDID010000313         1263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.322, whole genome shotgun sequence.
ACCESSION   DACDID010000313 DACDID010000000
VERSION     DACDID010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.322"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IV236_002813"
     CDS             <1..305
                     /locus_tag="IV236_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP8772948.1"
                     /translation="YENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNII
                     GSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            433..1116
                     /locus_tag="IV236_002814"
     CDS             433..1116
                     /locus_tag="IV236_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8772949.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT          422 a          208 c          275 g          358 t
ORIGIN      
        1 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
       61 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      121 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      181 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      241 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      301 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      361 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      421 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      481 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      541 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      601 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      661 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      721 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
      781 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
      841 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
      901 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
      961 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1021 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1081 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1141 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1201 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1261 gtt
//
LOCUS       DACDID010000314         1233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.323, whole genome shotgun sequence.
ACCESSION   DACDID010000314 DACDID010000000
VERSION     DACDID010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.323"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..667
                     /locus_tag="IV236_002815"
     CDS             134..667
                     /locus_tag="IV236_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772950.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            679..882
                     /locus_tag="IV236_002816"
     CDS             679..882
                     /locus_tag="IV236_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772951.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT          403 a          174 c          261 g          395 t
ORIGIN      
        1 ctaactatac cacattatca aaaatataaa ttatctttac taaataaaat attgacaagc
       61 ctataccttt tgttttatga ttttcacaaa gaaaacgttt tctatatatt tttctgtatc
      121 aggaggaaat tctatgaaaa aatttacttt aggggtagct ttgttaacta ttggggcagt
      181 tgttttaccg tcaactacta tagtttatgc ttcagaaaat tcccaagtgg tatcattaaa
      241 cgaaaacaat gctactgaaa atgtagttat tgagtacact gaagatggta tggtcgtaac
      301 taaatatgag gatggttata gtgcggaaga ttttgcaaat tcttcagata tcatgacaag
      361 agctgcagtt gcaagatggg gcaaaaagga atatacaaat attgctgtaa ccactggagt
      421 agctgctaat gctattaatg ctgccttttg ggcaggaggg aaagctagca cttctatgtt
      481 tggtatccca ggttgggcaa ttggtgcttt gttaacaggt gcaagttgga ctaaaaaagg
      541 tgcttctcca gggaatgcag ttgcaaaaga atgggacaaa aatggtaatg gctggattgg
      601 attttattac caaaggggat atgatgcagc tggaagagtt gtagctacta gatattcaac
      661 gttataagtt aaggagtgat gtttttgata tctaaatcat taaaatcatt aaaaacattt
      721 actaaaaaga atccgcaaat agctactatt cgtattttta tatttgctat agctgttgct
      781 ataattgtta atatgctaag cggagggcaa ttatacaaat ctggtttatt tttagtgtta
      841 atcttagttg tcttagacat aataaagtct aaaaaaaaat aaatcgatac attttagaag
      901 aatagggttg tttgaatatt acaaagcaac aatttcacct tcggtgaaat aagcgaaact
      961 ttacaaaaag tggctagcaa atgctagcct ttttttgtat tgtggagcgt ggattttgag
     1021 ggggattcaa gggggagctt gcgctccttt gacaagcggt tatttgcacc aaatacggag
     1081 gtatttggct agcaaatata gagcttgcca aaccctttta gtgagcttta ctcattgtac
     1141 ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga
     1201 cttatgagcg aacaacatca aaatttggct agc
//
LOCUS       DACDID010000315         1226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDID010000315 DACDID010000000
VERSION     DACDID010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.324"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1106
                     /locus_tag="IV236_002817"
                     /pseudo
     CDS             1..1106
                     /locus_tag="IV236_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          410 a          236 c          207 g          373 t
ORIGIN      
        1 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
       61 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
      121 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
      181 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
      241 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
      301 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
      361 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
      421 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
      481 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
      541 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
      601 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
      661 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
      721 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
      781 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
      841 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
      901 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
      961 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
     1021 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
     1081 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
     1141 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
     1201 tttttgactt attttcagag gaaata
//
LOCUS       DACDID010000316         1200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.325, whole genome shotgun sequence.
ACCESSION   DACDID010000316 DACDID010000000
VERSION     DACDID010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.325"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..1194
                     /locus_tag="IV236_002818"
     CDS             37..1194
                     /locus_tag="IV236_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772952.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          452 a          179 c          217 g          352 t
ORIGIN      
        1 atccttagaa aatcttagtc aaaaaattat ataagtttgc gtacgattca aagaaaatta
       61 agagaactta atactatttt gaaaaactat catttatctt gcagcttgaa aaaaggtaat
      121 ccgatagaag gagaagaata ccgtatccga tacttttttc acttgatgta ttggcagtca
      181 ttcgacgaaa atcattctga tatattatca agtataaaaa ataaacgtca actattagaa
      241 aaatttgcga aacatgcgcc atatacgcgt agtatagata gaaagaaatg gatcagccta
      301 ctaagaattt cgttgcaacg aatgaaacaa ggccactcga ttacgtatgt cccagaagag
      361 atgaacaaat ttgtgcatcc attgataaca aaagaagagt ttacgcatta catactagag
      421 cctttttttg aagcaaactt ctggtcgatg aatcacatca ctccaacaga gcaagcttac
      481 ctttatttta tgtttggtgt aatgaacacc tatttggaag aagatttgtt ggatactcca
      541 gataaagtac aagaaagttg gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa
      601 aacttcaaga tttgttttac aacttcagaa aaaaattatc tatatgtcaa tttggcaatg
      661 atccatttgt attcaagggt ttttgggaca aaaaagaagg tggatgcttt tggaaaatca
      721 gcagaagaca gagattttca gcatatattt ccagtgatgt atccaatcat gcaacagttt
      781 tttagtgggg tagctcgcag aattccagag cttaatactt attatgaaaa gaacagacgg
      841 ttgatttttc aatactgtat gttagtcagg gatattttta taaaacacga acaacctgtc
      901 atattctata ttcaatcaaa gtatggtaaa acacaggaat tatgggcaaa gaaaaggata
      961 ttatctatga ctgaacgccc aataaaatat agtgcatccg ctgatcagtt acctgatatt
     1021 gtcattagcg attatcctat cgacaaaaag gcatatgatc aaacgaatac acagatattt
     1081 tactggaatg gacaacctac aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa
     1141 atcaggaatg agaaaaatag atccatattg gaaaaattgg atttcgttaa ttaagaaaag
//
LOCUS       DACDID010000317         1200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDID010000317 DACDID010000000
VERSION     DACDID010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.326"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..713
                     /locus_tag="IV236_002819"
     CDS             <1..713
                     /locus_tag="IV236_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8772953.1"
                     /translation="EKKYLPGEQLPTEEELCKIYGVSKITIRKAFKLLAESGTVERLR
                     GKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLANKLQINVTD
                     RVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVKATSSIM
                     EINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYVIEVTE
                     NITLDTVHKNDSDPKVID"
     gene            complement(811..>1200)
                     /locus_tag="IV236_002820"
     CDS             complement(811..>1200)
                     /locus_tag="IV236_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8772954.1"
                     /translation="NILIEQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCL
                     ISGRDLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRF
                     SQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT          427 a          180 c          184 g          409 t
ORIGIN      
        1 gtgaaaaaaa atatcttcca ggagagcaat tacctacaga agaagaatta tgtaaaattt
       61 atggagtaag taagattacc attcgaaaag cttttaaact tttagcagaa agtggaacag
      121 ttgaacgatt aagggggaaa gggacctttg taactcaaaa aaaagaaagt ttaaaattag
      181 ctggagttca tggatttagc gattctcttt ctacccgagg acataaaatt agatatactg
      241 ttttacattc taaaattata aaggctgatg agtttttagc taataaatta caaatcaatg
      301 taactgatag agtttacaat attaaacgtt taatgtggga agacggtgcc ccgatgggaa
      361 ttgataattt tttcgcatca gaagaaaaat ttccaacttt atttgaaaaa ggtagccaat
      421 atacttcatt atataaactt ctagaagatg actatgatgt caaagctacg agctctatta
      481 tggaaataaa cggtttatct gcaacacctg agctttccga cttattgcaa tgtatagtag
      541 gagatccgct atttatttta gaaaaggttg gcaaagataa aaataatcaa ccatttcatt
      601 attctcaatc cacagttcgt tgtgatagaa ttagttatgt tattgaagtc acagaaaata
      661 tcactttaga tacggtgcat aaaaatgatt cagatcctaa agtaatagat tgatattaaa
      721 agaaatcata cccaaagcaa tcaattttga ttagatttgg gcatgatttt tatttttact
      781 aatattctct aaatacatca aaaaacttat ttatattcca aatttcacct gtacttcttg
      841 tcgcagatat ttaatatcgt aaataacatc atctgctaga cttacatctt gagaaaaacg
      901 tttatcttta attgcaagaa ccttcatatt tgctctttta ccagctttta taccattata
      961 agaatcttct atcaccagac attcttcaac tggaattctg agctgttctg aagctttaag
     1021 gaatatagta ggattaggtt tactttctgg caaatctctt ccgctaatta aacaatcaaa
     1081 atatgactct attttacaag tggtaacaac ttcatttata gtatccattg gactggaaga
     1141 agcaattgcc atttttattt ttttcttttt taaccattca agctgttcaa ttagtatatt
//
LOCUS       DACDID010000318         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDID010000318 DACDID010000000
VERSION     DACDID010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.328"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV236_002821"
     CDS             <1..1076
                     /locus_tag="IV236_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8772955.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDID010000319         1189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.329, whole genome shotgun sequence.
ACCESSION   DACDID010000319 DACDID010000000
VERSION     DACDID010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.329"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(82..744)
                     /locus_tag="IV236_002822"
     CDS             complement(82..744)
                     /locus_tag="IV236_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8772956.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1108..1189
                     /locus_tag="IV236_002823"
                     /pseudo
     CDS             1108..1189
                     /locus_tag="IV236_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
BASE COUNT          395 a          228 c          161 g          405 t
ORIGIN      
        1 tattcattgt tatttatact aaagcacccg atagctctga aaacaatcac aaattcaact
       61 tttcaaagcc acagctttaa gttattttgt ccagacaacc cccattgccc aacccatttt
      121 atggaattgg catccaggca acaacttttc atataaatcg taataatttt gttcagatag
      181 gtacttatct gaagctaaat gctcaagcca tgatttagat gtgttgtgat tataaattct
      241 aatcgcattt tttattccaa atttgaaaca atgcggtaaa aactcttgga ttgcccctaa
      301 tttataaaca taggtaggcg gtgtctcgac ttcagataca ttatcaagaa taactattct
      361 tccttcctca ttcaacagtt ccttcatttg ctgtattacg ctggctatat catccaaatg
      421 atgaaaggtt gttcggctta caataaaatc aaacttctca ttaaaattaa gttgttctgc
      481 attcatattc agatagaccg tatttgttag ttgacgttta gatttggcaa gatcgagcat
      541 ttgattagaa atatcaatcc ctaccacttc atcataataa cttgctaatt tctccactaa
      601 caaacccgag ccacatccga tatctaatgc tctgcctttc tttggagaca tattagacac
      661 aaagaatgaa taatcattca aaagctcatt cacgaaatcg taatcttctg caaccttatc
      721 aaactgtgat tctattgtat tcaaaaagat cccccattcc tactttatcg acattctttc
      781 attacttacc actttagatg ttttttcgtt ggggataaaa cttcccttta gacaatttta
      841 tccaaagaca atacaacagt gcaactttat taaagtcact gtcctttatc gcagccttta
      901 ctttttagta aagacagtgg cttctcttat caagtttcaa aacatattat tttgaagaaa
      961 acgtccatct gaagtgtcaa gtgcaaaatt acatataaag gtttattcta aaatgaaaag
     1021 atgatacaat catattcagt tacataagga ggtttcaatt atgtgcacca gtatcgcagt
     1081 agtagaaatt actttatctc attcataatg aaaaaaatgg aaaggagata aaagtatggg
     1141 tactttttct atatttgtta ttaataaagt tcgttatcaa ccaaatcaa
//
LOCUS       DACDID010000320         1177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDID010000320 DACDID010000000
VERSION     DACDID010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.330"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1177)
                     /locus_tag="IV236_002824"
                     /pseudo
     CDS             complement(83..1177)
                     /locus_tag="IV236_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          302 a          241 c          209 g          425 t
ORIGIN      
        1 tcccattcaa cgcaaaaaaa taaatagtga cggtttttcc ttcacgataa agctttgctg
       61 atccggtatt cccgtttgta actgttaagg ttacgacttc atatttgttg tcgtctgatg
      121 tcaatacaga ttttcctttt gattgaatac catcttcgaa atttttcaat ccttcgatgg
      181 attgcggttc agtcaaacta accgtatcat tcaagccttt ttcagtatat tcaggcgtga
      241 tgtcccagct gtagtcgttc ggattgttgc tatctttcaa tccttcacca aagtatttaa
      301 actgactaat attcggggtt cgggtgtcgc ctttttcaat tttgagccag tcaatttggc
      361 atgcgccggc cgtttcttta ggcaactgaa aaatgcgaag gtctttaggc aaacccggtt
      421 caagtttcgt tggtgtgaat gttagagacc atacgtctgt caatccctca actggtttta
      481 gttctccaaa attaacgttc caataattat atgctacaaa ggtttgactt gcgggttttg
      541 ttcctttaag cgtgatagta tacgtttgac ctattataag ctcttcttcc gtgttacctt
      601 tatatatttc gtacgcgcta gatttaattg ggaacgtcgt acctttattt gcaatattct
      661 cacccaacga tgttttacct acccagtaag ggtcatctag taagttaggc tggtacggtg
      721 tggctgttga gccttcttcg attttgatgt cgtacaactt aaatccgcca tttattttat
      781 ccctgtcaac aaagctaata ctcatataaa atcgatctaa atttgtgatt tgataattaa
      841 cgttagctgt acctttgatt gttatttctt tccctacatc atctgttgta atatttgtac
      901 tatttgcttc caataatatc ttttctcctg gtgatgtacg atacaccaaa cgtaatttat
      961 caatagctcc tgtagttcct tcatcaaatc gaactttcgc actcagagta taggttttcc
     1021 cactagcaag ctgaggtgta ttaatacgcg taaacataat aatgcttcct gtaccatcag
     1081 aagtaaaatg tagcttttcg ttatccgaag ttagtgaccc gtggtaacca acgttaaaat
     1141 cgctcgattt tagtttggac attaaattcg gcttacc
//
LOCUS       DACDID010000321         1152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.331, whole genome shotgun sequence.
ACCESSION   DACDID010000321 DACDID010000000
VERSION     DACDID010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.331"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IV236_002825"
     CDS             <1..354
                     /locus_tag="IV236_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8772957.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IV236_002826"
     CDS             355..903
                     /locus_tag="IV236_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772958.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          452 a          148 c          243 g          309 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcatga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag ga
//
LOCUS       DACDID010000322         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDID010000322 DACDID010000000
VERSION     DACDID010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.332"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV236_002827"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV236_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8772959.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDID010000323         1135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDID010000323 DACDID010000000
VERSION     DACDID010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.333"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(137..997)
                     /locus_tag="IV236_002828"
     CDS             complement(137..997)
                     /locus_tag="IV236_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772960.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          339 a          216 c          166 g          414 t
ORIGIN      
        1 aagcatgctt tgtaacgtat tttacgataa ttacgacttt attttattac tctatcttgc
       61 tgccataccc gtctatccct aatcatcttt ggtgaatttt ttcaaagtta gcataacaaa
      121 aataagtatt ttttccttag ttaatcaata gtatcatttt tttcatatga tttaattcat
      181 caattaattt tttgttttta ttaattttag caaaagctag tgcatcttga atcaattcga
      241 gcacttctgc cttcgactta tttttagcct gtgcgttctt cgctaattgg gcagcagctc
      301 gtgctaagta ataagtcaaa tggttatccg aacaaatatc gattgcgtaa tttagtagtg
      361 catcacttgt ttccaaatca ccgtcttgcg caaaaaaaac accacaatga aagacgatat
      421 ttaacacccg ccagatatct tctgttgtac gaatcggata cgtataaatt ttttcaaaca
      481 ctcgattgaa ataaacttct gctttttcca actctttttc tctagcatag accattccaa
      541 tacctgtata tgccaataaa cgaaacaacg tgttggctgt ttctgccatc aacaactgat
      601 caaaatggaa caacacatcc gtaattggtg cattcgtaaa aatcatttca aatcctttta
      661 gatagtagta acgtgcaatc agctcagact cgtcaattac cttgggatca atggctgcta
      721 aaatctgtgt caattgttca tattcactcg taatcagtga aaactctgct ttttctagct
      781 gttcatcaag ttgtgtattg atcacgttca tcgaagaaaa aagttcaact aacggaagat
      841 tgattcgatt acataatttg attaaaattt ttaacgaagg gacttgacca ttgttctcaa
      901 atttacttaa tgttgcttgt gtacaaattc cttcagacac ttctttttga gataagttgg
      961 cagcctttct tgccgtaata aatttttcaa tgttcattat tttctctcca atcagacctc
     1021 acacgaaaat catttctgtc tcgattttag catgttttct ttgggaatgc tttaggttct
     1081 gttgcaaagt tttaaataaa gaataaagtc ccttacggta tctatgattt aagct
//
LOCUS       DACDID010000324         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.334, whole genome shotgun sequence.
ACCESSION   DACDID010000324 DACDID010000000
VERSION     DACDID010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.334"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IV236_002829"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IV236_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8772961.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DACDID010000325         1106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.335, whole genome shotgun sequence.
ACCESSION   DACDID010000325 DACDID010000000
VERSION     DACDID010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.335"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..432
                     /locus_tag="IV236_002830"
     CDS             22..432
                     /locus_tag="IV236_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8772962.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            446..802
                     /locus_tag="IV236_002831"
     CDS             446..802
                     /locus_tag="IV236_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8772963.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          389 a          163 c          211 g          343 t
ORIGIN      
        1 taattaaaaa tgagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
       61 agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt
      121 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
      181 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
      241 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
      301 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
      361 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
      421 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
      481 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
      541 cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
      601 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
      661 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
      721 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
      781 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
      841 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
      901 ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata
      961 tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt
     1021 aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta
     1081 gaaatgacat gataagataa ctgagt
//
LOCUS       DACDID010000326         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDID010000326 DACDID010000000
VERSION     DACDID010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.337"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..642
                     /locus_tag="IV236_002832"
     CDS             196..642
                     /locus_tag="IV236_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772964.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            644..781
                     /locus_tag="IV236_002833"
     CDS             644..781
                     /locus_tag="IV236_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8772965.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            819..>1090
                     /locus_tag="IV236_002834"
     CDS             819..>1090
                     /locus_tag="IV236_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772966.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          405 a          172 c          222 g          291 t
ORIGIN      
        1 gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc
       61 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
      121 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
      181 ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca
      241 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa
      301 ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg
      361 aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca
      421 atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg
      481 ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta
      541 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
      601 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
      661 tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
      721 gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga gcgcatctta
      781 attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta ggggaatggg
      841 gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta ataaaaccaa
      901 ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac aatttagatt
      961 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
     1021 agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac acgcaacaaa
     1081 ttaaaacttt
//
LOCUS       DACDID010000327         1087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.338, whole genome shotgun sequence.
ACCESSION   DACDID010000327 DACDID010000000
VERSION     DACDID010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.338"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IV236_002835"
     CDS             191..445
                     /locus_tag="IV236_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8772967.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IV236_002836"
     CDS             446..763
                     /locus_tag="IV236_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8772968.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          383 a          169 c          220 g          315 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgta
//
LOCUS       DACDID010000328         1086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.339, whole genome shotgun sequence.
ACCESSION   DACDID010000328 DACDID010000000
VERSION     DACDID010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.339"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..639
                     /locus_tag="IV236_002837"
     CDS             193..639
                     /locus_tag="IV236_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772969.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            641..778
                     /locus_tag="IV236_002838"
     CDS             641..778
                     /locus_tag="IV236_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8772970.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            815..>1086
                     /locus_tag="IV236_002839"
     CDS             815..>1086
                     /locus_tag="IV236_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772971.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          397 a          179 c          220 g          290 t
ORIGIN      
        1 gcttattcat ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta
       61 caaatcaatc aagccttacg gattcgagca aatcattgtg aatgacgaag aacatttacc
      121 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
      181 ggagggaaat aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga
      241 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg
      301 gttcgttcag atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac
      361 caatggatta ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg
      421 tacgaggctc ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta
      481 agcgatgtag cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca
      541 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
      601 aaagagcaac taaatccagc ttcaaaagga ggtgagtagt atgtttagtt ttagcgatgt
      661 gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact
      721 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
      781 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
      841 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
      901 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
      961 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1021 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1081 aacttt
//
LOCUS       DACDID010000329         1071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.340, whole genome shotgun sequence.
ACCESSION   DACDID010000329 DACDID010000000
VERSION     DACDID010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.340"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1071
                     /locus_tag="IV236_002840"
     CDS             <1..>1071
                     /locus_tag="IV236_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8772972.1"
                     /translation="EFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILILKE
                     IIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLNTND
                     SLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRLMSE
                     VVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLKFTD
                     YEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPIDEQA
                     YVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVLHLN
                     AAVKRLKLGVNIYNPYTDKIKYS"
BASE COUNT          423 a          156 c          179 g          313 t
ORIGIN      
        1 cgaatttagc gaacggcaaa tgaaaatact atactttttg attgaaaatg agcacacaac
       61 ttacaaagaa ttatcagaac aattatatgt atctacgaaa acaattagca aagatatttt
      121 aattttgaaa gaaataatcc aaaaaatgcc agatgtacaa atgttggttc aacctaaaaa
      181 aggcgtttcg cttattggaa aaaaagaaac acttcgaaat ttattatcca gtcataaaat
      241 caatatcgca gactctccgg aggaccgtct aacttatgtg tttgctaaat tattgaatac
      301 taatgattct ctaaagattc aaaatcttgc tgacgaatta tacgtgagca ggtcgacggt
      361 agaaagcaca atgaaagatg ttagaaaaag actaaacaag catgggatgt ccttagtttc
      421 agatcgaaat ggcgtgcgca tcaaagctga tgaaaaaagt aaaagaaggt tgatgtcaga
      481 agttgtgaac tattattggg gtggctttac agcatctaac agtaaaacag atctcttaga
      541 cttgaatatc aatttcattg gtgaagcgaa taatttctta aataaggaca tattgaaaaa
      601 agtagccgaa ttgcttaatc gatacatgaa taagacacaa ttgaaattta cagattatga
      661 atttcaaact ttagctatcc accttgctat tgcgatagaa aggattgaaa caagcaattt
      721 cttatcacgt agtaataatt ctgaagaaga aatctcaaag aatacactta ttttaatcaa
      781 aatgattgaa aaagcatttt caattattgt gccaatagat gaacaagctt atgttgacat
      841 tcacattact tcgattgaaa aaaatgctat gaatcacgac ttagatcaaa cgaagctttc
      901 ctatttagat atgggtgttg agctacaaga gttagtaaga attaaattaa atgaatttta
      961 tcctgatgaa gaattgatca ataatctagt attacatctc aatgcagcag ttaaaagact
     1021 caaattggga gtaaatatct ataaccctta tacggacaaa atcaaatatt c
//
LOCUS       DACDID010000330         1069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.341, whole genome shotgun sequence.
ACCESSION   DACDID010000330 DACDID010000000
VERSION     DACDID010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.341"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IV236_002841"
     CDS             <1..969
                     /locus_tag="IV236_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8772973.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DACDID010000331         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.342, whole genome shotgun sequence.
ACCESSION   DACDID010000331 DACDID010000000
VERSION     DACDID010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.342"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..963
                     /locus_tag="IV236_002842"
     CDS             4..963
                     /locus_tag="IV236_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8772974.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DACDID010000332         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.343, whole genome shotgun sequence.
ACCESSION   DACDID010000332 DACDID010000000
VERSION     DACDID010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.343"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IV236_002843"
     CDS             complement(<1..357)
                     /locus_tag="IV236_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8772975.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKAL"
BASE COUNT          356 a          199 c          186 g          321 t
ORIGIN      
        1 taaagctttt tcgtaaactt gtatgtctac atatggatct ttagcagttc cacgtatttt
       61 aaactcacta aacataacga atctatctat atcgagccca ttagcacttc ttttaccaaa
      121 acgtaaatcc attaatttat cataagtcat ttctaaatca ttaatgaaac gattattagc
      181 agttgcttta taattactta aatcttttag ttggtcaaaa gtaagacgga ctgttttatc
      241 gcctttttcg cgcattctag acacaataga gaaaaataga ttcatttcaa ctggtgtaaa
      301 tttccgcaac ggaatggtat ttaattcatg atggtatttc acgatttcat ttgccaaaga
      361 tattcctcct tactccttca atggatacag acgttataaa caacgttttg tctgtgataa
      421 tgcatattat aacatacgtg agtgcaaaaa aagagtttaa aagcagacaa aatgtagttt
      481 aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta gtttaaaagc agacaaaatg
      541 tagtttataa aacgctgtat ccattgaagg agtaaggaca cagccgacgg taaagaggtt
      601 taaaaaaagg tttaaaaaga gattataaaa gagggtgaca aaatttcccg cctaaaaacc
      661 acttcttttt tctaccaaca ggttcgcgca tttttcaaaa ctgccaattc aaaaccttgc
      721 tccctacggt cgctaaaaaa gtgtgatcgc tatgctctca cattgtactg cgcagtcgcc
      781 taaaggctcc tttgctaaaa acaagtcagt aacggaggtg ggggaagcct ctgatagctc
      841 gtctaagtcg ttttaagaca gtcttgggta aaaaacctta tcttgttctt aaagcgcctt
      901 acaggcggtc agggcaagcc ctaacaaccc gaaaataagg gatacttaaa aacaccacac
      961 agattttatt ttgtaaggta taataacttg tcgcctatcc gacagtcttg cgactggaag
     1021 gaggtgtatc taatgtttga acggttcatt tgcccccttc tt
//
LOCUS       DACDID010000333         1031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.344, whole genome shotgun sequence.
ACCESSION   DACDID010000333 DACDID010000000
VERSION     DACDID010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.344"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1000)
                     /locus_tag="IV236_002844"
     CDS             complement(<1..1000)
                     /locus_tag="IV236_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8772976.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNS"
BASE COUNT          314 a          209 c          168 g          340 t
ORIGIN      
        1 gactatttag acgacgggtc tggcaaacag gttcgccagt ggtaacctga tatcctttta
       61 gctctgctaa acaaacacta agcccatttg taaaaaaagt taaatcattg cgataatctt
      121 gaatacatcg agcaggaatt tctccaataa taatgacctc attatttttc agttgagtat
      181 ttacgatatt tgcacaatat ttgggagcat cgttatatgc ccgtgaaaga tattcctgtg
      241 gtgcataaac tttaaaacta agatatggct ctaacaattc tgttccagct tttctaaagg
      301 cttgctccag tacaatagga gtaagcatcc gaaaatctgc tggagtacta acagggctat
      361 agtataaacc gtacttaaaa cagattttac aatccgtcac attccaacca tataatcctt
      421 gttcgcaacc atagcgtatc ccttccataa ctgcattttg aaatgattga tttaagtatc
      481 caagagaaac cgagctctca tactgcattc cacttcccaa cggaagcggt gatacagata
      541 aaccaatgga agcccagaaa ggatttggcg gcacttcgat gtgaatggta tattctgcat
      601 tttttaacgg tctctccata taaatgactg taggctcttt tagttctatc tccacatgat
      661 acttttcttg caacagtgca ctaatcactt ccatttgtac tttccctaag aaagaaagta
      721 taatttcatg tgtcgtagaa tccacgtaat atcgtagaag cggatcacta tctgagattt
      781 ccaaaagggc atcaagcaac atttctctct gttcaggttt actcggttca acagttgttt
      841 gtagtagagg gtgcggattt tcaatctttt ttctctgtgg caatagtttt gtatctccaa
      901 gaacactatt taacttcaaa aactcatttt gcaaaataac aatttctcca gaataagctc
      961 tatcaatctt acataattca ccatttattg aagtatacat ttctgtaact tttatttttt
     1021 ctttttctga t
//
LOCUS       DACDID010000334         1029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.345, whole genome shotgun sequence.
ACCESSION   DACDID010000334 DACDID010000000
VERSION     DACDID010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.345"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IV236_002845"
     CDS             287..694
                     /locus_tag="IV236_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772977.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..>1029
                     /locus_tag="IV236_002846"
     CDS             708..>1029
                     /locus_tag="IV236_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772978.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDY"
BASE COUNT          387 a          130 c          176 g          336 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacg
//
LOCUS       DACDID010000335         1022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.346, whole genome shotgun sequence.
ACCESSION   DACDID010000335 DACDID010000000
VERSION     DACDID010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.346"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..555
                     /locus_tag="IV236_002847"
     CDS             <1..555
                     /locus_tag="IV236_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8772979.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRN
                     ISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          335 a          181 c          190 g          316 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttttcgta atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tagaagcacg tggtgctttt
      241 gcgaaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataagca aatcaagaaa tacagccacc gcaaggaaca gttccaaaac
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa
      541 ccgatggaga attagaaaca atctacagta gggaagaacc atttacacaa aattattgac
      601 gctcccaagc taaaactcct attaatcgat ctactaaaaa taaataaaaa aattcttttc
      661 aatttttatt tatggtatga cataaataaa gtaaagttat tcttctaggt taatcttatt
      721 aatactcttt ctcactagtg ttgcatgcaa cactagtgag aaagagtatt aaatatactg
      781 caaggcaaga catttggtaa ttgaagaata acaagcaaaa aaatagcagt caggtctctt
      841 gacaaagtat agttgctatt ctttttttag gccctacttt aatgaaattt ttgtttggag
      901 ggaacgactt cattcctttg attatttcag ttgaatttaa tagagaagcc tggcgtctat
      961 taagaaatac ttttcttgat gagcgtcaat ttttttaacg gaaatctatc ctatactaaa
     1021 tt
//
LOCUS       DACDID010000336         1015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.347, whole genome shotgun sequence.
ACCESSION   DACDID010000336 DACDID010000000
VERSION     DACDID010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.347"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..430
                     /locus_tag="IV236_002848"
     CDS             <1..430
                     /locus_tag="IV236_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8772980.1"
                     /translation="EVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGY
                     LIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNE
                     ARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            497..991
                     /locus_tag="IV236_002849"
     CDS             497..991
                     /locus_tag="IV236_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8772981.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
BASE COUNT          365 a          138 c          206 g          306 t
ORIGIN      
        1 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
       61 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
      121 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
      181 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
      241 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
      301 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
      361 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
      421 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
      481 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
      541 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
      601 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
      661 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
      721 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
      781 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
      841 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
      901 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
      961 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aatta
//
LOCUS       DACDID010000337          999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.348, whole genome shotgun sequence.
ACCESSION   DACDID010000337 DACDID010000000
VERSION     DACDID010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.348"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>999
                     /locus_tag="IV236_002850"
     CDS             <1..>999
                     /locus_tag="IV236_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8772982.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFS"
BASE COUNT          418 a           85 c          166 g          330 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagtt
//
LOCUS       DACDID010000338          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.349, whole genome shotgun sequence.
ACCESSION   DACDID010000338 DACDID010000000
VERSION     DACDID010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.349"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IV236_002851"
     CDS             complement(26..913)
                     /locus_tag="IV236_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8772983.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDID010000339          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.350, whole genome shotgun sequence.
ACCESSION   DACDID010000339 DACDID010000000
VERSION     DACDID010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.350"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV236_002852"
     rRNA            complement(<1..>994)
                     /locus_tag="IV236_002852"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDID010000340          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.351, whole genome shotgun sequence.
ACCESSION   DACDID010000340 DACDID010000000
VERSION     DACDID010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.351"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IV236_002853"
     CDS             complement(<1..461)
                     /locus_tag="IV236_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8772984.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(746..952)
                     /locus_tag="IV236_002854"
     CDS             complement(746..952)
                     /locus_tag="IV236_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8772985.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          313 a          184 c          128 g          342 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aagaagccgt
      721 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
      781 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
      841 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
      901 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
      961 ctttcaa
//
LOCUS       DACDID010000341          965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.352, whole genome shotgun sequence.
ACCESSION   DACDID010000341 DACDID010000000
VERSION     DACDID010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.352"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..412
                     /locus_tag="IV236_002855"
     CDS             56..412
                     /locus_tag="IV236_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8772986.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            402..668
                     /locus_tag="IV236_002856"
     CDS             402..668
                     /locus_tag="IV236_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772987.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          402 a          124 c          187 g          252 t
ORIGIN      
        1 tcgaattttt atgttatata ctataatata aaatcaatat atgatataat tgggtatgga
       61 aaaaatatct tttgaaacat ttaaaagacc taatggtcat gatgaatttt tagaatggtt
      121 ggaaacatta ccaaaaaaag atagtgcgaa gcttttacgt acaatagaag aaacagaaaa
      181 gaatgggatg ctagtagctc aacggttgaa atgggtgaaa aaattagaca ctaatttgta
      241 cgaattaaga agtaaggtag gatcaaacat ccaaagagct atctatttcc atgtggtcaa
      301 tggaaggtat gtcattaccc atggatttac taaaaaaacg gagaaaacac caataagtga
      361 acttaaacat gcaaaagaac ttagagagga gtggttcaca aatgatgatt aaaaaattga
      421 ttgaagaaaa gcaaaaagac aaagattttg cagaagcata tgaccgtgaa ggaagaaaac
      481 tcgaaactgc ggttgctctt tatcatgcac gggaggaagc aggcctaact caagcggagc
      541 ttgcacaaaa agcacataca actcaatcta ctattgctcg aattgagaga ggggataacg
      601 tatctttcga taaacttgat gatatcgcta gggcattagg caaaaagctg gtagtagaat
      661 ttgcttaaaa gcgagttgaa aatatacctg taataggctc ctaactaatc aattagttta
      721 gtgggccttt ttgtactaaa tagataaaag ttgtcctata gtgtaaaagt caataaattt
      781 tatacaaaaa ttactttaaa gagacataaa gagaaaggta caataaatga aaaccataaa
      841 tctaataaaa gtatttatag catcaccagg tgatatagca gcaaagagga atgaagtaga
      901 agaaaccata tatagatgga atatagaaaa tacagataat acaaaagtaa ttttgatgcc
      961 aattc
//
LOCUS       DACDID010000342          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.353, whole genome shotgun sequence.
ACCESSION   DACDID010000342 DACDID010000000
VERSION     DACDID010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.353"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IV236_002857"
     CDS             complement(55..789)
                     /locus_tag="IV236_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8772988.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          331 a          197 c          180 g          251 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactcttt
//
LOCUS       DACDID010000343          949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.354, whole genome shotgun sequence.
ACCESSION   DACDID010000343 DACDID010000000
VERSION     DACDID010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.354"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>949
                     /gene="uidA"
                     /locus_tag="IV236_002858"
     CDS             <1..>949
                     /gene="uidA"
                     /locus_tag="IV236_002858"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8772989.1"
                     /translation="NGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWI
                     GANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEI
                     RTENAHKMAIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKR
                     PVTIVTYLKSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCP
                     DTPIMFTEYGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADF
                     ETSQGILRVQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNF"
BASE COUNT          343 a          125 c          205 g          276 t
ORIGIN      
        1 gaatgggaag tttttgatta ataataaacc tttttacttt aagggatttg ggaagcatga
       61 ggacacgtat attaatggtc gtggtttgaa tgaagcagta aatatcaaag attttaattt
      121 aatgaaatgg atcggtgcta attcatttag gacttcacat tatccttatt cagaagaaat
      181 tatgcgctta gcagatcgag aaggtattgt agttattgat gaagtacctg cagtaggatt
      241 acatttgaat ttcatggcta ctttattaga tgacaacgta gaaaaacatg atacttggaa
      301 agaaatacgg acagaaaatg ctcacaaaat ggcgattaag gaattgatag aacgcgacaa
      361 gaaccatccg agtgtcgtaa tgtggtctat tgcaaatgaa ccagattcgg attctgaagg
      421 cgctaaagaa tattttgaac ctttagttga attgactaaa gaacttgacc ctcagaagag
      481 acctgtaaca atcgttactt acttaaagtc aacaccagat atttgtaaag taggagatat
      541 tgtcgatgtt ttatgtttaa atcgctatta tggttggtat gtttcaggtg gagacctaaa
      601 acatgcaaaa tatctgttga aaaaagagct agatggatgg atggaaagat gtcccgatac
      661 gccaataatg tttacagaat atggagcaga tacagtagct ggacttcatg atacagtacc
      721 aacaatgttt acagaagagt atcaagtggg ttattacgaa gcaaatcacg aagtaataga
      781 taaatgtgac aattttgttg gtgaacaagt gtggaatttt gcagattttg aaacaagtca
      841 agggatttta agagtgcaag gaaataagaa aggaatcttt actagagata gaaagcctaa
      901 aatgattgct cactatctaa agaaacgatg gtcatcaata ccaaatttc
//
LOCUS       DACDID010000344          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.355, whole genome shotgun sequence.
ACCESSION   DACDID010000344 DACDID010000000
VERSION     DACDID010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.355"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            220..456
                     /locus_tag="IV236_002859"
     CDS             220..456
                     /locus_tag="IV236_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8772990.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            475..690
                     /locus_tag="IV236_002860"
     CDS             475..690
                     /locus_tag="IV236_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8772991.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          305 a          127 c          215 g          298 t
ORIGIN      
        1 aaattctcaa tatagcttta atacagtgaa aatattgtct cataattcca caaaaacttt
       61 gaaaacagtt cttgaacact aactttttca ttcctgaatc aaatttattt tggaaatttt
      121 actattcaag aataaacttg ataagtttca ggaagatttt tactgttaat caaatacgta
      181 tgttacagta gcaccgtaaa aaatattatt taaagaagcg tgtttaaaca tgcaaaatgt
      241 aaaggaacta agtattgtgg aaatgcacaa actgttggtg gaggcaataa tgcttgggga
      301 aaattggggc aagttgtagg tggacttacc acaggggcag taggtggtgc aggtttagga
      361 acagcaattt gcggaccagc ttgcggtgtt gttggagggt tatatggagc tgtcgctgga
      421 ggtgctgcag ctggctggga tgctcgaaaa aaataaaaaa ggagaataag tagtatgcat
      481 attaaaaaca caaaaacaac atttatatta tcttcagaag agttgaaaaa tattcaaggt
      541 gggagtgccg tgggagtttt aggcactact ttttctgggg caacagcagg agtaaaactt
      601 tgttcagccg gaggacctta tgcaatagct gcttgtggtg taggtggagc tttattaggt
      661 gctggattca gcatgtggac cggtgcgtaa acaattaaaa aatatcaaac ctcaagattg
      721 gtttgtgtcg attacgaaca taggtatgtt ctttatcttt tcaaaagttt ggaatggaca
      781 gaacttttta ctaaagcttc ttgtgctagt ttttataatt atattaaatt tttatctttt
      841 attaaagtca gataaaaatt ctttggaaat gtaatggtag agatattttt acaaaaggac
      901 ttgcagcgct gtagttggtg ctgcaggagg acctatttcg gccgt
//
LOCUS       DACDID010000345          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.356, whole genome shotgun sequence.
ACCESSION   DACDID010000345 DACDID010000000
VERSION     DACDID010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.356"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(398..>945)
                     /locus_tag="IV236_002861"
     CDS             complement(398..>945)
                     /locus_tag="IV236_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8772992.1"
                     /translation="NGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTK
                     DQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLP
                     HRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSEL
                     ARNFTLGKAQKIKEKLKESTV"
BASE COUNT          325 a          169 c          111 g          340 t
ORIGIN      
        1 ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc
       61 ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa
      121 attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct
      181 ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga
      241 ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta
      301 taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt
      361 ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact
      421 tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat
      481 atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc
      541 gactaattct tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt
      601 cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa
      661 aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt
      721 gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa
      781 tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc
      841 ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt
      901 gaacatcata ttttctgatt acttctgcta attctatacc gttaa
//
LOCUS       DACDID010000346          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.357, whole genome shotgun sequence.
ACCESSION   DACDID010000346 DACDID010000000
VERSION     DACDID010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.357"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..>943)
                     /locus_tag="IV236_002862"
     CDS             complement(37..>943)
                     /locus_tag="IV236_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772993.1"
                     /translation="DLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSD
                     IGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQ
                     FNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYIND
                     LTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSL
                     YINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQ
                     EEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          305 a          161 c          145 g          332 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tct
//
LOCUS       DACDID010000347          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.358, whole genome shotgun sequence.
ACCESSION   DACDID010000347 DACDID010000000
VERSION     DACDID010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.358"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            286..919
                     /locus_tag="IV236_002863"
                     /pseudo
     CDS             286..919
                     /locus_tag="IV236_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          308 a          172 c          173 g          266 t
ORIGIN      
        1 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
       61 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      121 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      181 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      241 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      301 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      361 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      421 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      481 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      541 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      601 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      661 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      721 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      781 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      841 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      901 tcagacttat tttttacct
//
LOCUS       DACDID010000348          906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.359, whole genome shotgun sequence.
ACCESSION   DACDID010000348 DACDID010000000
VERSION     DACDID010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.359"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..725
                     /locus_tag="IV236_002864"
     CDS             <1..725
                     /locus_tag="IV236_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8772994.1"
                     /translation="YLEIELFKHGYKTIICNSQNDPAKEREYIEMLEANQVDGIISSS
                     HNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEGGKLAAKTLQKNGCQNTIMITGN
                     DNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRREMEIKSTIINHKPDGIFVSDDLT
                     AILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQLTTIRQPIDEIASLIVDILLKK
                     IKGEKTNKDYILPISILSGGSI"
BASE COUNT          328 a          140 c          139 g          299 t
ORIGIN      
        1 agtatctcga aatagagtta tttaaacatg gatataaaac gattatttgc aatagtcaaa
       61 atgatcctgc taaagagaga gaatacattg aaatgcttga agccaaccaa gttgatggca
      121 taatctcatc aagccataat cttggaattg ctgattatga gcgtgtgaga gcacctattg
      181 ttgcttttga tagaaattta gctcctaatg tttccattgt ttcttcagat aactttgaag
      241 ggggaaaact tgcagctaag actttacaaa aaaatggttg tcaaaatacc attatgatta
      301 caggaaatga taacacggat tctcccacag aattacgtgc actaggtttt tcttttcaaa
      361 atccaaatgg aaaaattttc aaaattccta ataatctgtc tactatccgt cgtgaaatgg
      421 aaataaagtc aactattata aatcacaaac cagatggtat ttttgtttct gatgatttaa
      481 cagccattct cactatgaaa attgctcatc aacttaaact taatatccca gaagatttaa
      541 aaattattgg atacgatggt acctctttta ttgagaaatt tttcccacaa ttgacaacaa
      601 taagacaacc aattgatgaa attgcaagtc ttattgttga tattcttctg aaaaaaatta
      661 aaggcgaaaa gacaaataag gattatattt taccaatctc aatactttct ggaggaagta
      721 tttaacgtta aattaatttt ttaatgtcta taaaattcgt tagaaaatac aatttgtaga
      781 tatccaattg atttgtaaac aaaaaggagt tgtcaacttg tagtgacccc caaaagttag
      841 acttttttgg agtggagaaa tccactcctt ttttcatgct ctaaaatgat atttttacac
      901 aactgt
//
LOCUS       DACDID010000349          898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.360, whole genome shotgun sequence.
ACCESSION   DACDID010000349 DACDID010000000
VERSION     DACDID010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.360"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..>898
                     /locus_tag="IV236_002865"
     CDS             79..>898
                     /locus_tag="IV236_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8772995.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          309 a          136 c          156 g          297 t
ORIGIN      
        1 atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt
       61 ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat
      121 aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg
      181 caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca
      241 ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat
      301 ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa
      361 agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg
      421 tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt
      481 tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa
      541 tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt
      601 tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt
      661 gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta
      721 aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac
      781 gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct
      841 agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttca
//
LOCUS       DACDID010000350          894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.361, whole genome shotgun sequence.
ACCESSION   DACDID010000350 DACDID010000000
VERSION     DACDID010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.361"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(600..824)
                     /locus_tag="IV236_002866"
     CDS             complement(600..824)
                     /locus_tag="IV236_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772996.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          319 a          127 c          152 g          296 t
ORIGIN      
        1 ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc
       61 ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct tttttaattt
      121 gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa
      181 ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta ttgtgggtac
      241 tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat tctatcaggt
      301 ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga ttttgatctg
      361 aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa
      421 tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca acagtacctc
      481 tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa ttgttgttat
      541 accacatata aggatttgta atagctaaaa acagacagga aagttgaaat ctgtgaatct
      601 tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa taaaaatcct
      661 gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt catagccata
      721 taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc tcgaaaagtt
      781 ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata gtcatagcct
      841 ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg tacg
//
LOCUS       DACDID010000351          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.362, whole genome shotgun sequence.
ACCESSION   DACDID010000351 DACDID010000000
VERSION     DACDID010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.362"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..191
                     /locus_tag="IV236_002867"
     CDS             6..191
                     /locus_tag="IV236_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8772997.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            203..451
                     /locus_tag="IV236_002868"
     CDS             203..451
                     /locus_tag="IV236_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772998.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            482..661
                     /locus_tag="IV236_002869"
     CDS             482..661
                     /locus_tag="IV236_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8772999.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
BASE COUNT          319 a          122 c          184 g          265 t
ORIGIN      
        1 tttttatgtc tgagaattta gacttaaaaa ttcgagcgga gatgagaaaa agaagaatga
       61 ctttcaaaga actagctgcg cttatcggta tttcaggagc ttatttatca gatattctaa
      121 acggcaatcg tgatggaaag aaagcacaac agcatatcga aacagtgaaa aaaatattgg
      181 acatccgata ggggggtagg cgatgacaaa actaaaaaaa caagattttg taaaaaaata
      241 caattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt
      301 ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag aattatacga
      361 taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa cacctaaaga
      421 atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga ggtgaaggtt
      481 aatgggtaaa ttcaacagag cattggtatt cagcgcaccg ctaatcatct acgctttagg
      541 actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt ggatttttat
      601 ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg gggctgacta
      661 aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt aattctttca
      721 ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg atttgggttc
      781 tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa ttaattttcc
      841 ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
//
LOCUS       DACDID010000352          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.363, whole genome shotgun sequence.
ACCESSION   DACDID010000352 DACDID010000000
VERSION     DACDID010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.363"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>887
                     /locus_tag="IV236_002870"
     CDS             <1..>887
                     /locus_tag="IV236_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8773000.1"
                     /translation="IAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLT
                     KRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNR
                     ANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPD
                     LFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIFLINQL"
BASE COUNT          299 a          174 c          168 g          246 t
ORIGIN      
        1 attgcgaaaa attgtcaggt atctttgacc accgttattc gtacgttgaa agagtttaaa
       61 agctacttac caaagcaatc taagaggatt ctccccagag tattgatggt tgatgaattt
      121 cgttcgcacg cttccataga agataaaatg agctttattt gcgcagatgg cgaaacagga
      181 caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat taacaagcta tttcttaggc
      241 tgcgctaatc ctgaagaagt aaaattttta gtcacagaca tgaacgccgc ctacttccag
      301 cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg atcggtttca tattatcaaa
      361 cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa tgaatgaact tcgtaaagta
      421 ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc gctttttgct aaaaaatcgt
      481 gcaaacatca accattatga atacaaaaca tggaaaagtt ttcgagcacc aaaataccca
      541 tttcttacgg aagcgatgat gattgatcga ttgcttggct tttctacgtc cctgaaggag
      601 gcgtatccct attttcatga attagttgaa gcctttcgag acaaagaccc tgacttattt
      661 ttctccttat tggcagaact tcccgaaacg ttagatgacg gctttcggga aaagctccaa
      721 aaccttctga cctatgaaga aggcatcacc aacgcgatga tctatcctta caccaatgga
      781 aaaatagaag cgaagaatac ccatataaag acaatgaaac gagtatccta cggatttaaa
      841 tcatttgaga acatgagaat tagaatcttt ttgatcaatc aattaat
//
LOCUS       DACDID010000353          882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.364, whole genome shotgun sequence.
ACCESSION   DACDID010000353 DACDID010000000
VERSION     DACDID010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.364"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            150..518
                     /locus_tag="IV236_002871"
     CDS             150..518
                     /locus_tag="IV236_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8773001.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            538..762
                     /locus_tag="IV236_002872"
     CDS             538..762
                     /locus_tag="IV236_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773002.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
BASE COUNT          339 a          117 c          185 g          241 t
ORIGIN      
        1 taaaaaaagt ttgtggagga aagcaatcct agaaactatt gctttatttt tcttgaatag
       61 tatcaaacac aaggtgtttg cgctttcgtg atataatgga atcagtttat agacaggaaa
      121 gaaaatcaaa tagaaagaag ggtcaaatga tgatacaaaa aacacgtgtg atgctttatg
      181 tcaatgacgt agagatgatt tgccgattct ttgtagaaaa aattggtgca gaaatttctg
      241 aaacaatcga attgccagaa gaatttaaga gtattgtact cagcatctca aaagaattag
      301 aattaggcgt attcccaaaa gtatttgtgc agaagttttc accagaggta ttaggaccac
      361 cgccttcttt agtatttttt acggatgaat ttgaaactat ctacgagaac atggaagaac
      421 caggagaaat cacagataac aatggcatct tgactttcaa tttttctgac cctgaaggca
      481 attattttgt tataggaaaa gctgagtcgt ctgattgaat aaaaaggagg aaaaacagtg
      541 gatctaatgg gaagattaga aggttatttg aagtatcggc gagcaaaaca tagtgagaag
      601 cttcgagcaa agaatctcaa atcaggaaaa aagaagacaa aaggctgggg aaaaatggtc
      661 gatacaggtt ttggcggact aaataatctg aatcctgtcg atacaaaaga attcatcaaa
      721 gaagaacaga aacgaaatgg taaaatcaaa tcacactatt gatttttgag actagaatga
      781 agaaacttta ttaacacaat aaagaaattc ttgtaatcca aagtgaatgt tcatgaactt
      841 tgagataaag ggatttaatc agatggaacg ttcggatttt gc
//
LOCUS       DACDID010000354          875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.366, whole genome shotgun sequence.
ACCESSION   DACDID010000354 DACDID010000000
VERSION     DACDID010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.366"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..674
                     /locus_tag="IV236_002873"
                     /pseudo
     CDS             60..674
                     /locus_tag="IV236_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          321 a          137 c          155 g          262 t
ORIGIN      
        1 agagttctaa cgtatgatta gggtgtatta taaacgttat agaaaagtag ggaataaatt
       61 tgaaaattgc ttatattaga gtttcatcca tcgatcaaaa tgaacaacgt caaattgaag
      121 aaatgaaaaa gtttggcgct gaaaaaattt ttatcgaaaa acaatctgga gcgacgatta
      181 gtcatcggcc gattttccag gaagctttag attttgtcag agagcaagat atttttattg
      241 tagaagccat tgatcgactt ggacgcaatt atgacgagat tatcgattca gtgaattatc
      301 taaagaagaa gaatgtgcga cttattatca ccagtttacc gatcatggct gaagcaattg
      361 gaaatcctct cctagataaa ttcatcaaag acttaatcat ttaaatctca gccatgattg
      421 ctgaacaaga acgtactgaa tcgaaacgac gccaggcaca aggaattaaa atcgctaaag
      481 caaacggcgt ctataaaggt cgtcctaagt tatacagtgc agacaccaaa gatcctcaac
      541 gtcgccttgt ctataggagt atcgttcagg atcttgagaa tggggttgct atctcaaaga
      601 ttgcaacaga ctacaatgtg actaggcaaa caatttaccg aataaaaaaa gaaatcgacc
      661 aactaattgt atgatttctt ttttgaatca cgtaattatt aattagcttt gtcatcaata
      721 cagacaatca ttttagattc atctagctaa gttgattaca tttatgttaa aaagaattta
      781 gttaacataa atataattga tgcataaatc gtccttattt aaaggcattt aacattgaca
      841 acttagtata atgtatctta tgtaaactaa taaga
//
LOCUS       DACDID010000355          874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.367, whole genome shotgun sequence.
ACCESSION   DACDID010000355 DACDID010000000
VERSION     DACDID010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.367"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(620..865)
                     /locus_tag="IV236_002874"
     CDS             complement(620..865)
                     /locus_tag="IV236_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8773003.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          291 a          167 c          125 g          291 t
ORIGIN      
        1 atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga cctgttaaat
       61 ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc aaaacaagcc
      121 cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg tatatttttt
      181 tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc ctactagtta
      241 gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt atctgaaaca
      301 gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt cagatacatt
      361 tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag cctatgcttt
      421 ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat gaatgaaaaa
      481 ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat gtgaacaaat
      541 aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg aatcgtacaa
      601 ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac tcatcatcta
      661 acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc gcttctggat
      721 gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata ttaggaattg
      781 gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca gaaacaaacc
      841 actcattgga aaagacatga atcaaatcat cact
//
LOCUS       DACDID010000356          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.368, whole genome shotgun sequence.
ACCESSION   DACDID010000356 DACDID010000000
VERSION     DACDID010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.368"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IV236_002875"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IV236_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IV236_002876"
     CDS             complement(545..739)
                     /locus_tag="IV236_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773004.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          300 a          147 c          136 g          283 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataaca
//
LOCUS       DACDID010000357          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.369, whole genome shotgun sequence.
ACCESSION   DACDID010000357 DACDID010000000
VERSION     DACDID010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.369"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(213..443)
                     /locus_tag="IV236_002877"
     CDS             complement(213..443)
                     /locus_tag="IV236_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8773005.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..>845)
                     /locus_tag="IV236_002878"
     CDS             complement(673..>845)
                     /locus_tag="IV236_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8773006.1"
                     /translation="LQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELRE
                     KVLRSIIDKSIE"
BASE COUNT          309 a          132 c          126 g          278 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaa
//
LOCUS       DACDID010000358          839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.370, whole genome shotgun sequence.
ACCESSION   DACDID010000358 DACDID010000000
VERSION     DACDID010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.370"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..331)
                     /locus_tag="IV236_002879"
     CDS             complement(86..331)
                     /locus_tag="IV236_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8773007.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(325..726)
                     /locus_tag="IV236_002880"
     CDS             complement(325..726)
                     /locus_tag="IV236_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8773008.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          262 a          138 c          118 g          321 t
ORIGIN      
        1 aggttttctg tttaaaagaa tctactaatc ctttaactat tagaagattc ttttaaacag
       61 aataagtgga aaggggactt tttagttagt taattttaaa ttttaagatc gcttctatca
      121 atttaatttc tagctgtctt agcatatact catctacata ggtaataaat tgccccgcct
      181 cattaaatcc atttgtcaaa cataacttag ctatataact atgataatgt ttgagtatca
      241 tcttgattga aattggatct tcttctagag cagattggat aatggataga ggaactaatg
      301 gatactgccg ttttttgggg ttgtctacca taagtcatac cccattttta attgttcaac
      361 tgccttcttt cgttgatacc agataccact tgtactcata ttgaggattt taccgatttc
      421 agtatcatta aaacctgcaa aataataaag taggataacc tttcttctta tttccgataa
      481 atgatcaatg gctgccgata acttttgatc tgaaattaat atttccattc caaataagaa
      541 atagacatct tgttcatcaa aatgagtttg ggagtactta ccttcatttg aattcgtcaa
      601 tggttcctca ttatctgtta tgttaatgtt tcgttttcga atagacttag tttcgtttcg
      661 taatactttt ttacagtagg catcaaattg atgttgtata gatttctcat tgtaattttg
      721 ttgcatgata aatatgctcc ttttttcccc tttccactaa tacaacaatc agaaagcgcc
      781 tttttttcga aaaaactaga aaaaaatatc cttctttaaa cagaaaatct ttttataaa
//
LOCUS       DACDID010000359          837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.371, whole genome shotgun sequence.
ACCESSION   DACDID010000359 DACDID010000000
VERSION     DACDID010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.371"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..355
                     /locus_tag="IV236_002881"
     CDS             86..355
                     /locus_tag="IV236_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8773009.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="IV236_002882"
     CDS             348..605
                     /locus_tag="IV236_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8773010.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          323 a           94 c          156 g          264 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatact
//
LOCUS       DACDID010000360          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.372, whole genome shotgun sequence.
ACCESSION   DACDID010000360 DACDID010000000
VERSION     DACDID010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.372"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IV236_002883"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IV236_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8773011.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDID010000361          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.373, whole genome shotgun sequence.
ACCESSION   DACDID010000361 DACDID010000000
VERSION     DACDID010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.373"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..413)
                     /locus_tag="IV236_002884"
     CDS             complement(<1..413)
                     /locus_tag="IV236_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8773012.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYS"
     gene            complement(431..751)
                     /locus_tag="IV236_002885"
     CDS             complement(431..751)
                     /locus_tag="IV236_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8773013.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          271 a          145 c          111 g          284 t
ORIGIN      
        1 taagaatatt ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta
       61 ttctgaatag aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt
      121 tcgttctcaa tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca
      181 aattttgagt gaaaagttgt agaagtatcg tacaaaactg aatattcaaa atgagaagcg
      241 gtgaaatgcg caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt
      301 tcattcaaaa aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg
      361 taaactactt tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc
      421 atcactccaa ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg
      481 tctccttaga tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca
      541 catgtttagg ggtttctctt tcttctctac ctagaaggta gtctacagag actccaaagt
      601 aatcagctaa ccgagctaac tcaatagaat taggagtttg tcttttccaa cttcctaaat
      661 atccattcga atacccgaaa ttcatttcga gctgtctaat ggataatcct ttttcttttg
      721 ctaactcctt tattatttcg taagtattca ttgataaatc aacctttctg aatgcttaca
      781 aaaaaagttt agataaataa tgtaaaaata g
//
LOCUS       DACDID010000362          802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.374, whole genome shotgun sequence.
ACCESSION   DACDID010000362 DACDID010000000
VERSION     DACDID010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.374"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>802
                     /locus_tag="IV236_002886"
     CDS             <1..>802
                     /locus_tag="IV236_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773014.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNG"
BASE COUNT          296 a          154 c          160 g          192 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aa
//
LOCUS       DACDID010000363          786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.375, whole genome shotgun sequence.
ACCESSION   DACDID010000363 DACDID010000000
VERSION     DACDID010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.375"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..>786
                     /locus_tag="IV236_002887"
     CDS             80..>786
                     /locus_tag="IV236_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8773015.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFV"
BASE COUNT          274 a          103 c          163 g          246 t
ORIGIN      
        1 tataattttg atgtggattt tatcggttta tttatgttaa agtatagatg aagatatgtt
       61 tttgattgga gggtcaccat tgaaaaaaat tgcgaagtat atggaaattt acttagatat
      121 aaagaaaaaa attgtaaatg gagagtataa aattggtgag aaacttccta caggagatga
      181 attggcagtt atttatcata caagcaagtt aacagtaaag aaaggcgtag acttacttgt
      241 ttccgaagga gttttgcgaa gtagaagtgg gtatgggact gaagtcgtca gaacaccaat
      301 tgataactca cgagttcttg gtccaaacga aggtttgttt agcgtggttg gtgaagaaca
      361 cgtagattca gaaatccata ctttctcaat tgaacttcca tcgaaaaaag tagctgataa
      421 attaagtatc agtgtaaaag actatgtata taatattgta cgaagccgtt ttattgattg
      481 tcaaccatac tcgattgagc aaacattcat gccattagca gtcataccag gcttggaacc
      541 aaaacattta aagaaatctg tatatgctta tatcacaaat gaactgggtc tggaaataaa
      601 gacttcgcac atacgtatca aaggagatac tgcgaatgaa tttgacgcaa aaatattaaa
      661 aatagatcca ggtgaattta tgattgagat agacaaagtt gttgctctat cgtctggtaa
      721 acctttcgag tattcggtat cgagacattt atatcaagac tttgtttttg aagctgtttt
      781 tgttga
//
LOCUS       DACDID010000364          785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.376, whole genome shotgun sequence.
ACCESSION   DACDID010000364 DACDID010000000
VERSION     DACDID010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.376"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            154..390
                     /locus_tag="IV236_002888"
     CDS             154..390
                     /locus_tag="IV236_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8773016.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            387..785
                     /locus_tag="IV236_002889"
     CDS             387..785
                     /locus_tag="IV236_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8773017.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          285 a          119 c          141 g          240 t
ORIGIN      
        1 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
       61 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
      121 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
      181 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
      241 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
      301 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
      361 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
      421 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
      481 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
      541 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
      601 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
      661 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
      721 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
      781 aatga
//
LOCUS       DACDID010000365          782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.377, whole genome shotgun sequence.
ACCESSION   DACDID010000365 DACDID010000000
VERSION     DACDID010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.377"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>782
                     /locus_tag="IV236_002890"
     CDS             <1..>782
                     /locus_tag="IV236_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8773018.1"
                     /translation="ERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTS
                     KLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLG
                     ADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKE
                     GILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFA
                     LNEKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLVYIL"
BASE COUNT          301 a          102 c          140 g          239 t
ORIGIN      
        1 aagagagatt ctgtatacat tttaatgaag atgaaatcgc ttacgtaaca ctccatattc
       61 aatcactttt ggatcgttac aaacctgaca aaacgaaagt gatattagtt tgtagtagcg
      121 gatatggaac atcaaaatta ttagagcaaa gaataaataa cggttttgca gatatggttg
      181 agatagaaga tgtactaagc atcaatgaac tacaagatag aaaagtcact gatgaacttg
      241 ttataagcac gattccgatt gaaaatacaa attttcctgt tattgttgtt agtcctttga
      301 tgttaggggc agatgtcaga aaagtaaaac agtatctaca gttaaatgaa caaacagaag
      361 atgcttttct aaatttatta gataaacgat tttgcttttt tacccaccaa ataaaggata
      421 atcaagtgaa cgttttaaat ataattacca aaaaattggt aaaagaaaat tacgcgaaag
      481 aagggatact agagggagca atcaaaagag aaaaactatc ttcaacagcg atgaatgatt
      541 ttgctatgcc gcatatcgaa gtagatttta taaaaaagcc agtaatctgt gtctacgtta
      601 atccagaagg aattgattgg gatgggacac ttgttcattc tgttttcttt tttgcactta
      661 atgaaaaagt aaaacctgaa ttgaataaaa tttatgaaac attcaatgaa atcgcttcga
      721 atcatgagtt attagcaaat ttaaaaacga gttcgtcgtt tgaaaaatta gtttatatat
      781 tg
//
LOCUS       DACDID010000366          782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.378, whole genome shotgun sequence.
ACCESSION   DACDID010000366 DACDID010000000
VERSION     DACDID010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.378"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV236_002891"
     CDS             complement(<1..132)
                     /locus_tag="IV236_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8773019.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     "
     gene            290..553
                     /locus_tag="IV236_002892"
     CDS             290..553
                     /locus_tag="IV236_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773020.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            577..765
                     /locus_tag="IV236_002893"
     CDS             577..765
                     /locus_tag="IV236_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773021.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          304 a          100 c          157 g          221 t
ORIGIN      
        1 agtaaaatat acagcaggta aagaggccag taaaactgaa aacttctttt ttgtccattt
       61 tatgtaatag aaaagaatgc ttatcataat aaaaaaagga atataaaaaa caaattgacc
      121 gatcgcgatc atactattgc ctccgataaa taaagtccat gatacaaaat aacactttgt
      181 gcgaaatggc gcaagcagtt ttgtggattt atccggtaaa atctgtatcg attttgaaat
      241 tagaggagaa acttgctata atcaaagtga aagaaggagt gggattttca tggatcatgt
      301 agtgttagaa atcgatttgc atttgatcaa taatattcgg gtgatttact atgttgtaag
      361 taacagtgtc gaacaacgtg tactgacgaa taaaataaat ggtattttag caaagaaaga
      421 cgttcaccga ttcaataatg gtgaaggaag ttattatagt atcccagtag aaaaaattat
      481 ttatacgaca gtcaaagtca gagaagatct agataccaaa aaagcatatg aacctatttt
      541 tacaacttat tgattgacag cagatggagg aaaaaaatga atccatatga acaacttatt
      601 caagatttga tcgatggaaa aatcgaaaag attgatgtaa aaagagaaga attaatggct
      661 tttcgagaag cgtggctgaa gttggagaat cgtaaatatg tggtgggaaa agcctcactg
      721 aacggcaatg tgacttatca ttacgatccg acacgtttat tttagtatga agtattcaga
      781 aa
//
LOCUS       DACDID010000367          773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.379, whole genome shotgun sequence.
ACCESSION   DACDID010000367 DACDID010000000
VERSION     DACDID010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.379"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..574
                     /locus_tag="IV236_002894"
     CDS             <1..574
                     /locus_tag="IV236_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8773022.1"
                     /translation="IIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVS
                     MGTYAGMKVFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQN
                     ADFIFLDEPFVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRN
                     LIAHGSVESVFNEENLKKAYGDTIFIGEGE"
     gene            576..>773
                     /locus_tag="IV236_002895"
     CDS             576..>773
                     /locus_tag="IV236_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773023.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINF"
BASE COUNT          271 a           85 c          153 g          264 t
ORIGIN      
        1 tattatccct catcagggag aggtctccat taataatgaa gatatcaata aaaaactggt
       61 gaagattgca tatgtagagc aaaaagctga tattgatttt acttttccaa taaaagtaaa
      121 agaatgtgta tctatgggaa catatgccgg aatgaaagtt tttcaaagaa ttaaaaatgc
      181 agaatggcaa agagtttcaa aggctttgga aaaggtcgat atgggaaaat attctaatca
      241 tcaaatagga gaattatctg gaggacaatt tcaacgtgta cttttagcaa gatgtcttgc
      301 tcaaaatgcg gatttcatat ttttagatga accatttgta gggatagact tagttagtga
      361 acgaataatt atggatacgt taaaaaaatt gaaacatcag ggaaaaacag tgctaattgt
      421 gcatcatgat ttaagcaagg taaagaagta ttttgataat attattcttt tgaatcgaaa
      481 tctaattgct cacggaagtg tagaatctgt atttaatgaa gaaaatttaa aaaaagccta
      541 tggagatact atatttattg gagaggggga ataatatgct tcaaaatttt attaatggtc
      601 tgtatgactt tcacttttta cagaatgctc taattacatc tgtagttata gggattgttt
      661 ctggaacagt aggttgtttt attattttaa gaggaatgtc tcttatgggt gatgctattt
      721 ctcatgctgt tttgccagga gtagcaattt cttatatttt aggtattaat ttt
//
LOCUS       DACDID010000368          770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.380, whole genome shotgun sequence.
ACCESSION   DACDID010000368 DACDID010000000
VERSION     DACDID010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.380"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..>770)
                     /locus_tag="IV236_002896"
     CDS             complement(109..>770)
                     /locus_tag="IV236_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8773024.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          177 c          148 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc
//
LOCUS       DACDID010000369          762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.381, whole genome shotgun sequence.
ACCESSION   DACDID010000369 DACDID010000000
VERSION     DACDID010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.381"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV236_002897"
     CDS             <1..704
                     /locus_tag="IV236_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8773025.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          257 a          124 c          145 g          236 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ct
//
LOCUS       DACDID010000370          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.382, whole genome shotgun sequence.
ACCESSION   DACDID010000370 DACDID010000000
VERSION     DACDID010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.382"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          260 a          126 c          123 g          245 t
ORIGIN      
        1 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
       61 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
      121 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
      181 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
      241 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
      301 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
      361 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
      421 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
      481 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
      541 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
      601 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
      661 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
      721 acttatatgt tttagtgaac tacccccact tagc
//
LOCUS       DACDID010000371          744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.383, whole genome shotgun sequence.
ACCESSION   DACDID010000371 DACDID010000000
VERSION     DACDID010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.383"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>744
                     /locus_tag="IV236_002898"
     CDS             <1..>744
                     /locus_tag="IV236_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8773026.1"
                     /translation="IQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTA
                     TCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEK
                     IYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSN
                     IKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENN
                     ILFIVGTLNPHLEDVEFFSIEDMILGMNI"
BASE COUNT          276 a           91 c          134 g          243 t
ORIGIN      
        1 tattcaaata ggggatattt atttaacaaa aattcaaaaa aatgttttgg ttattttatt
       61 taagttaatg tgtagtaaag aatttcagca aaaacgttta gcactgattt tggctcatgg
      121 agatctaacg gcaacctgta tagcaagtac tgctaatagg atcgttgaga gatatatata
      181 cgacgccatt gatatgccac taatgacttc aagtagtgag attgcagaca aaattaatag
      241 atacttacaa gagtgtaccg gatatgaaga aattatttta cttgttgata tgggttcact
      301 ggagaaaatt tatctaaatt tggaactaga cgctcaaaaa aaattcggac tactaaacaa
      361 tgtttcaaca agaatggcta ttgatgttgc aaatcagata ttattagatt taccgcttaa
      421 acagattctt gaacaagtaa aagaaaatca aaatgtggat tatatttatg aatcatcaaa
      481 tattaagaag gatatgattg tctgttcttg tgcaagcgga ttaggaactt cgtttaaatt
      541 aaaaaagata cttgaagata gctttccaag ggatactgat cttgtcatag aaacgtgtga
      601 ctattactct ttagtgaatg aaaactattt atcggagctt cgtaaagaga acaatattct
      661 ttttattgtt ggaacactaa atccacatct agaagatgta gaattctttt caattgaaga
      721 tatgatttta ggaatgaata taag
//
LOCUS       DACDID010000372          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.384, whole genome shotgun sequence.
ACCESSION   DACDID010000372 DACDID010000000
VERSION     DACDID010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.384"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..734
                     /locus_tag="IV236_002899"
     CDS             <1..734
                     /locus_tag="IV236_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8773027.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DACDID010000373          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.385, whole genome shotgun sequence.
ACCESSION   DACDID010000373 DACDID010000000
VERSION     DACDID010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.385"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IV236_002900"
     rRNA            2..>733
                     /locus_tag="IV236_002900"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDID010000374          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.386, whole genome shotgun sequence.
ACCESSION   DACDID010000374 DACDID010000000
VERSION     DACDID010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.386"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..701
                     /locus_tag="IV236_002901"
     CDS             21..701
                     /locus_tag="IV236_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8773028.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          253 a          125 c          148 g          205 t
ORIGIN      
        1 agatagcagg aggaatgacg atgaatcatt ttaaaggaaa gcaatttcag caggatgtga
       61 ttattgtagc cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa
      121 tcttatatga tcgtggcatt aacgtttctc atacgacgat ttatcgttgg gtgcaagaat
      181 atggcaaact actctatcaa atttggaaaa agaaaaataa aaaatccttt tattcatgga
      241 aaatggatga aacgtacatc aaaattaaag gaaaatggca ttatttgtat cgagccatcg
      301 atgcagatgg tttaaccttg gatatttggt tacgtaaaaa acgggacaca caagcagcct
      361 atgcttttct taagcggtta gtgaagcagt ttgatgaacc gaaggttgta gtcacagata
      421 aagccccctc tattacaagt gcctttaaga aactaaaaga atacggcttt tatcaaggga
      481 cagaacatcg taccattaaa tacctgaata atttgattga acaagaccat cgtccagtaa
      541 agagacgcaa taaattctat cgaagtttac gcactgcctc taccacgatt aaaggcatgg
      601 aagccattcg aggattatat aagaaaaccc gaaaagaagg cactctcttc gggttttcgg
      661 tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg
      721 attttattct t
//
LOCUS       DACDID010000375          693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.388, whole genome shotgun sequence.
ACCESSION   DACDID010000375 DACDID010000000
VERSION     DACDID010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.388"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..381
                     /locus_tag="IV236_002902"
                     /pseudo
     CDS             1..381
                     /locus_tag="IV236_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
BASE COUNT          250 a           80 c          131 g          232 t
ORIGIN      
        1 gaaagtgacg aatttatagc attaagaaac tatgttgtta ataaaatatc accaattatt
       61 gattcgacag gtctatgttt attagatgaa ttcttggcgg atatcagtct atttataatg
      121 tttattgatg taattgaaga aacatcaaga aattttttgc tgtctaagtt cagaaaattt
      181 cgtatgaaaa atgataaaca aaaattattg gcaaatgaga tttggagtag tttagaagtc
      241 gaagaaagta aacaagaact tttgttgaaa tggatacttt ttcttgtaaa gaagttctat
      301 gtgaaaatac cagaaaccca ttgtctgata gttatgcacg gtaaaataac agcttctgcg
      361 atcgcaaggg aaacaaataa gttgctgaat acatatgttt atgaggcttt cgatatgcca
      421 attgaaggtg agactagtga cttaattaac ctaatcaaca atttttgtaa aagtatagat
      481 acgacgaatg gactcattct gctagttgat atgggatcat tggaacaaat gtatgaaaaa
      541 attgaatcca atgtaatagg tgatttggta attttaaaca atgtttctac agctttagcg
      601 attgaatgtg ggattcaagt ttgccaaaaa aagccaatta gtcattttta ccagatggat
      661 tttgattcgt ttcaagttaa agttcagtat tat
//
LOCUS       DACDID010000376          691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.389, whole genome shotgun sequence.
ACCESSION   DACDID010000376 DACDID010000000
VERSION     DACDID010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.389"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IV236_002903"
     CDS             132..344
                     /locus_tag="IV236_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773029.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>691
                     /locus_tag="IV236_002904"
     CDS             506..>691
                     /locus_tag="IV236_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773030.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          246 a          103 c          121 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct a
//
LOCUS       DACDID010000377          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.390, whole genome shotgun sequence.
ACCESSION   DACDID010000377 DACDID010000000
VERSION     DACDID010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.390"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..295
                     /locus_tag="IV236_002905"
     CDS             <1..295
                     /locus_tag="IV236_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HAP8773031.1"
                     /translation="EFRISLINNQLTFGKTPNYPWPQMFDKGLQRPLFLEIGKEYELK
                     LIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
     gene            318..>665
                     /locus_tag="IV236_002906"
     CDS             318..>665
                     /locus_tag="IV236_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8773032.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEI"
BASE COUNT          241 a           77 c          137 g          210 t
ORIGIN      
        1 cgaatttcgt atttcattga ttaataatca gcttactttc ggaaaaactc cgaactatcc
       61 ttggcctcaa atgtttgata aggggctgca acgaccacta tttttggaaa taggcaaaga
      121 atatgaacta aagttaattg ttgatgatac gatagctgtt ttatatattg ataatgttgc
      181 tttaagtact agaatgtatg ataaagtcgg aacagcatta agtatgactg ttacggaggg
      241 agcaatcaag gtagataaac tagactattc aataaattat gaatgtggat tttaaaacaa
      301 ttgaagggat gatgtaagtg aaattaaatg gtttacaaca tattggaatt ccaacaaaag
      361 attttggagc ttcaaaaatc ttttatgaat ccttagggtt tagtctgatt aatgaagaaa
      421 tgaatgtggg aagtaaagtc ggctttttcg agttgaatgg aacgataata gaaatatggg
      481 aagataaaac tactgagtgt agaggagcca ttgatcatct agcattagat acggatgaca
      541 tagatgaggt attcaattca attcaagatt taggatatga attattagat aaaaatatta
      601 tggaattacc gttctgggaa aatggaatcc gttactttaa tttctacgga ccaaatcatg
      661 agatt
//
LOCUS       DACDID010000378          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.391, whole genome shotgun sequence.
ACCESSION   DACDID010000378 DACDID010000000
VERSION     DACDID010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.391"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IV236_002907"
     CDS             complement(<1..270)
                     /locus_tag="IV236_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773033.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="IV236_002908"
     CDS             complement(276..>635)
                     /locus_tag="IV236_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8773034.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DACDID010000379          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.392, whole genome shotgun sequence.
ACCESSION   DACDID010000379 DACDID010000000
VERSION     DACDID010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.392"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            145..>596
                     /locus_tag="IV236_002909"
     CDS             145..>596
                     /locus_tag="IV236_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8773035.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGR"
BASE COUNT          195 a          118 c          125 g          158 t
ORIGIN      
        1 atatgaggta gaatactatc tattttcttt ttaacccaat tgttataatg gagagtgtaa
       61 aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac
      121 attcacagaa gagaggactt ccctatgaac gattttacta cagaaattct aaagactcta
      181 gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggagaaagc agtcaatacg
      241 cttctcaaaa cggagttaac ggctttccta gattacgaaa agtatgatcg cattggtttt
      301 aacacgggta attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtatggggaa
      361 cttcatctcc agattccgcg cgaccgcaat ggtgagttca agcaacagac tgttcctgct
      421 tatagacgga cgaatgacac gttagaggag accgtcattc acctcttccg aaaaggtatt
      481 accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcattacta cacgccccaa
      541 accatgtcca atataacaaa atcatttaca gaagaggtaa cggcgtttaa tgggcg
//
LOCUS       DACDID010000380          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.393, whole genome shotgun sequence.
ACCESSION   DACDID010000380 DACDID010000000
VERSION     DACDID010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.393"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>591
                     /locus_tag="IV236_002910"
     CDS             <1..>591
                     /locus_tag="IV236_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAP8773036.1"
                     /translation="EMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIY
                     VDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHT
                     FQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNII
                     KNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKK"
BASE COUNT          223 a           81 c          106 g          181 t
ORIGIN      
        1 ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat atgattttac
       61 tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt gctagtaata
      121 taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac aatgtaatag
      181 ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat attgaccaag
      241 caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa atgctaacta
      301 gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag agattagtag
      361 acttctactc taattttcag agttgtcctt acgagattga aagcagaggg aataataaaa
      421 acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta cccaacataa
      481 taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat atttgtgtac
      541 ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa g
//
LOCUS       DACDID010000381          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.394, whole genome shotgun sequence.
ACCESSION   DACDID010000381 DACDID010000000
VERSION     DACDID010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.394"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..>578
                     /locus_tag="IV236_002911"
     CDS             73..>578
                     /locus_tag="IV236_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773037.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVS"
BASE COUNT          210 a           81 c          121 g          166 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttc
//
LOCUS       DACDID010000382          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.395, whole genome shotgun sequence.
ACCESSION   DACDID010000382 DACDID010000000
VERSION     DACDID010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.395"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>576
                     /locus_tag="IV236_002912"
     CDS             <1..>576
                     /locus_tag="IV236_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773038.1"
                     /translation="MLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKI
                     PQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFS
                     HILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIM
                     SVVLVYKIGVQGATIATVICSYVIYVVRKRAV"
BASE COUNT          176 a           77 c          114 g          209 t
ORIGIN      
        1 aatgctaaat tactcgattc cattaattgc aactgttgtt ggatggtgga ttaatagtac
       61 agctgatagg tatgtggttt cctttatagt aggtgtctcg gcgaatggct tattgtctgt
      121 ggcatataaa atacctcaaa taattaatac tttgcaggga atatttatac aggcctggca
      181 aatttctgca attaaagaat atggagataa tgagactgca aaattttatg gaaatacatt
      241 tgttatgatt aatttgttaa tgtgcataac ctgttcaagt cttatcatat taactagacc
      301 gttttcacat attctttatg cgaaagactt ttatgaagcg tggaaatatg ttccattttt
      361 attaatagct aatgttttaa attgtgcatc aggtctgtta ggacctatct tatcagcaaa
      421 aaaagattct aaatccatga tgtggtctgc aatcataggg gcattagtta atgtgattat
      481 gagtgtagtc cttgtttata agataggggt tcaaggtgcc acaatagcca ctgttatatg
      541 ttcatatgta atatatgttg ttcgtaaacg agctgt
//
LOCUS       DACDID010000383          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.396, whole genome shotgun sequence.
ACCESSION   DACDID010000383 DACDID010000000
VERSION     DACDID010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.396"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..118)
                     /locus_tag="IV236_002913"
     CDS             complement(<1..118)
                     /locus_tag="IV236_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8773039.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVL"
     gene            complement(248..>556)
                     /locus_tag="IV236_002914"
     CDS             complement(248..>556)
                     /locus_tag="IV236_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8773040.1"
                     /translation="FENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTI
                     GMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS
                     "
BASE COUNT          203 a           94 c           74 g          185 t
ORIGIN      
        1 ctaaaactgt agcaataatg aaacacccca cttgaaatat atttcccttt cctaaaaaaa
       61 taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa cggattgatt tttgacactc
      121 tttcacctcc aaaaaataag atcgcactgt tatcttaagc tgataaaaat gcaccgttat
      181 tcagataaca gtgcgatctt atcaaaaagc ataactgaga acaattctca atgcaagctt
      241 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
      301 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
      361 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
      421 ttccgatagt ctttaactta tctagacaac agactgtctt tttcgtcaca aatgatgtaa
      481 ttttttcaac aatatcttga tatttggttc cagctgattg gaataaaatc aaatcgaata
      541 tacgtttatt ctcaaa
//
LOCUS       DACDID010000384          553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.397, whole genome shotgun sequence.
ACCESSION   DACDID010000384 DACDID010000000
VERSION     DACDID010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.397"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..>553
                     /locus_tag="IV236_002915"
     CDS             169..>553
                     /locus_tag="IV236_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8773041.1"
                     /translation="MKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDN
                     LQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIF
                     RSSGYSLFETYKDFFMMNLIKWGTTS"
BASE COUNT          186 a           92 c           92 g          183 t
ORIGIN      
        1 tatagacata cagatttaaa aaatgacctg gcgcaaataa cagaatatta tcaagaaatc
       61 agtgaaaaag aaccgaaatt tctatttata aaaaacaatc aacccattta tacatttatt
      121 ccttctatcc cctggatcga acattatccg gttgttgaaa tactgacatt gaaaaacagt
      181 atgtatttgg aaagaaatat tctcagcgga gaaatctatc caccgttaaa gatcaagata
      241 ggctctttgt cgaaagaaag gttatttgag ctgattgaag aaagtcagtt acgtgataac
      301 ttgcagttat catttccgta tactcagact gaagagatag ctgtcaaagt gttaagcaga
      361 tcatttcatt acttgataca gatattttta ctcactttct tttctttctt cttgttgagc
      421 tatgtcatgt tatgcatata ttttgtctac aattgtcgga aaatcgctat ctttcgttca
      481 agtggttatt cgctctttga aacgtataaa gatttcttca tgatgaacct catcaaatgg
      541 ggaacgacaa gtg
//
LOCUS       DACDID010000385          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.398, whole genome shotgun sequence.
ACCESSION   DACDID010000385 DACDID010000000
VERSION     DACDID010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.398"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..202)
                     /locus_tag="IV236_002916"
     CDS             complement(<1..202)
                     /locus_tag="IV236_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773042.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSN"
     gene            complement(300..545)
                     /locus_tag="IV236_002917"
     CDS             complement(300..545)
                     /locus_tag="IV236_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773043.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          159 a          107 c           78 g          202 t
ORIGIN      
        1 cattgctgtt tttaatctta tctagaatca cttcaaatgt ttcaaaggta tttacatcta
       61 actgacataa ccaaagcaag tacttctttt ctttgtaagt ccaatcaata ttacggtaag
      121 aggacattag attatggtac cttgctcttt gttcaactga agccaattcc cagcgttttt
      181 caaatatttt ttcttcttcc attattgatc cctcgcaaga cgctacctca aacttaaata
      241 atgggcagcg aaagtctaat tccttagatc taagtatagc aaacaaatat gaaaaaaact
      301 tagaaggtag taaaagttct tccgtctttt gctttgcttc tattcttaat gtgtttcgtt
      361 atcggtgccc tacaaagatt ttcattaatt acacactcat ttattgttgt gtattttctg
      421 acatttctct cttgatcaat tattttaatt ccatgggcat cctgatagtt cataggagag
      481 gagcggctat cagaaatagt tgcacctatc tcttgaactt cttttacaac ttctggaaaa
      541 tccatc
//
LOCUS       DACDID010000386          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.399, whole genome shotgun sequence.
ACCESSION   DACDID010000386 DACDID010000000
VERSION     DACDID010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.399"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IV236_002918"
     CDS             <1..75
                     /locus_tag="IV236_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8773044.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            253..>546
                     /locus_tag="IV236_002919"
     CDS             253..>546
                     /locus_tag="IV236_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773045.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT          205 a           94 c          114 g          133 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacgga
//
LOCUS       DACDID010000387          542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.400, whole genome shotgun sequence.
ACCESSION   DACDID010000387 DACDID010000000
VERSION     DACDID010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.400"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IV236_002920"
     CDS             <1..75
                     /locus_tag="IV236_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8773046.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..>542
                     /locus_tag="IV236_002921"
     CDS             249..>542
                     /locus_tag="IV236_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773047.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          213 a           94 c          105 g          130 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 ga
//
LOCUS       DACDID010000388          542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.401, whole genome shotgun sequence.
ACCESSION   DACDID010000388 DACDID010000000
VERSION     DACDID010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.401"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..515
                     /gene="spxA"
                     /locus_tag="IV236_002922"
     CDS             117..515
                     /gene="spxA"
                     /locus_tag="IV236_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8773048.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          189 a           87 c          110 g          156 t
ORIGIN      
        1 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
       61 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
      121 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
      181 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
      241 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
      301 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
      361 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
      421 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
      481 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
      541 ac
//
LOCUS       DACDID010000389          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.402, whole genome shotgun sequence.
ACCESSION   DACDID010000389 DACDID010000000
VERSION     DACDID010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.402"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>536
                     /locus_tag="IV236_002923"
     CDS             <1..>536
                     /locus_tag="IV236_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP8773049.1"
                     /translation="MEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVN
                     FEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVR
                     DNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRD
                     ELSELAKKVKVTFLTDPR"
BASE COUNT          212 a           55 c          108 g          161 t
ORIGIN      
        1 atatggaaga actgagaaaa tttctctcag acaaaaaaat tctactgatt gcaccaggaa
       61 aaagttctgt agatgaggta gaaagaataa aagagtttgt taccaaagaa gaagtcgtaa
      121 taattagtgt aaattttgaa tattctcata tagatataga ttacgtattt ttaagtaatt
      181 taagacgttt tcatgaatta ccgaaagaaa gcagaactaa atgtattgtt acatcaaata
      241 tacaagcatc ggatgtatat ttacggacga gttataaaaa gttgttatct gaaagggaag
      301 ttgtaagaga caatgcagga ttaatggcaa ttagattttt agccgatatg aatgtggatg
      361 aaatattctt agctggattt gatggatatt cacataatga agatgagaat tatggtgagc
      421 aatccatgga aattataaca aaatttgcat tattagatgc aatgaacgtg caaatgagag
      481 atgaattgtc agaattagct aaaaaagtaa aggtgacatt tttaacggat ccacgt
//
LOCUS       DACDID010000390          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.403, whole genome shotgun sequence.
ACCESSION   DACDID010000390 DACDID010000000
VERSION     DACDID010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.403"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV236_002924"
     tRNA            complement(5..90)
                     /locus_tag="IV236_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV236_002925"
     tRNA            complement(107..178)
                     /locus_tag="IV236_002925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV236_002926"
     tRNA            complement(190..262)
                     /locus_tag="IV236_002926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV236_002927"
     tRNA            complement(280..361)
                     /locus_tag="IV236_002927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV236_002928"
     tRNA            complement(369..441)
                     /locus_tag="IV236_002928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV236_002929"
     tRNA            complement(445..517)
                     /locus_tag="IV236_002929"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDID010000391          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.404, whole genome shotgun sequence.
ACCESSION   DACDID010000391 DACDID010000000
VERSION     DACDID010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.404"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV236_002930"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV236_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8773050.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDID010000392          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.405, whole genome shotgun sequence.
ACCESSION   DACDID010000392 DACDID010000000
VERSION     DACDID010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.405"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..445)
                     /locus_tag="IV236_002931"
     CDS             complement(<1..445)
                     /locus_tag="IV236_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8773051.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKG"
BASE COUNT          146 a           88 c           82 g          205 t
ORIGIN      
        1 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
       61 tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta taactctgaa
      121 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
      181 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
      241 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
      301 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
      361 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
      421 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
      481 tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DACDID010000393          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.406, whole genome shotgun sequence.
ACCESSION   DACDID010000393 DACDID010000000
VERSION     DACDID010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.406"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..>517
                     /locus_tag="IV236_002932"
     CDS             21..>517
                     /locus_tag="IV236_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8773052.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          189 a           94 c           86 g          148 t
ORIGIN      
        1 acagaaaaga ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca
       61 ctaaaggcga tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc
      121 tacggactga attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt
      181 caggtaattc gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa
      241 cattggaaat acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca
      301 aaagaacaaa cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga
      361 tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca
      421 tgtccaacat gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa
      481 atgataagta tgtcgctatt tttatggacg ctactta
//
LOCUS       DACDID010000394          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.407, whole genome shotgun sequence.
ACCESSION   DACDID010000394 DACDID010000000
VERSION     DACDID010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.407"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..>515)
                     /locus_tag="IV236_002933"
     CDS             complement(107..>515)
                     /locus_tag="IV236_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8773053.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DACDID010000395          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.408, whole genome shotgun sequence.
ACCESSION   DACDID010000395 DACDID010000000
VERSION     DACDID010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.408"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV236_002934"
     CDS             complement(<1..132)
                     /locus_tag="IV236_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8773054.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDID010000396          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.409, whole genome shotgun sequence.
ACCESSION   DACDID010000396 DACDID010000000
VERSION     DACDID010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.409"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..>512
                     /locus_tag="IV236_002935"
     CDS             23..>512
                     /locus_tag="IV236_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP8773055.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVM"
BASE COUNT          211 a           62 c          111 g          128 t
ORIGIN      
        1 accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
       61 aactacgagc ttacaagtgg cagaaagttt caaaaagaaa cataagcatg tactagaagc
      121 aattgaatca ataaaaagat cggtcgaaaa ttcggccaat gttgaagatg ggtccaattt
      181 tggacagatg tttgtggaag ggaacgagcc agactcatac ggaagaagtc ggagagttta
      241 tttcatgaat agagacggat tttccttgct agctatggga ttcactggaa gtaaagcaat
      301 aaatttcaaa ctaaaattta ttgaagcttt caacgaaatg gaagatgtta ttcggaagaa
      361 tactgttcct caaacaattg aagacatgat gatctatcaa ttagaagaaa tgaaagatgt
      421 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
      481 aattaagcaa aaaggaaata tgaaagttat gg
//
LOCUS       DACDID010000397          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.410, whole genome shotgun sequence.
ACCESSION   DACDID010000397 DACDID010000000
VERSION     DACDID010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.410"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IV236_002936"
                     /pseudo
     CDS             1..510
                     /locus_tag="IV236_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDID010000398          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.411, whole genome shotgun sequence.
ACCESSION   DACDID010000398 DACDID010000000
VERSION     DACDID010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.411"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..>506
                     /locus_tag="IV236_002937"
     CDS             44..>506
                     /locus_tag="IV236_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP8773056.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVM"
BASE COUNT          207 a           61 c          101 g          137 t
ORIGIN      
        1 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
       61 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
      121 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
      181 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
      241 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
      301 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
      361 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
      421 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
      481 gcaaaaagga aatatgaaag ttatgg
//
LOCUS       DACDID010000399          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.412, whole genome shotgun sequence.
ACCESSION   DACDID010000399 DACDID010000000
VERSION     DACDID010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.412"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          182 a           81 c           81 g          160 t
ORIGIN      
        1 atttttaaat agaaagacat ttgggttaaa ttgtgagata ataataaaga agagtttaaa
       61 gcgcacccca aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag
      121 ccattaaaaa gcataccata tttttgaaaa aaagtgagaa aaaaggctta caattggagt
      181 ggtagttaat tagtgactta tttttgattt tatagcactg atactataaa atatagatat
      241 catcatatta cacaatctta atactaactt aaaaaatatc tcctttcaca agtatggtga
      301 taaaattcgt tccgggctac ctttttaggt agcctacttt aatctttata cctttctgga
      361 tcaacgaaag tatactttat atagtcataa cgccgatgat cgctacgtgc gtccggcacg
      421 tcagtcatga atcggctaca ctagactaga cagaaaaaat aaggtgtgta gaataataaa
      481 aaacacactg gaggattttt catg
//
LOCUS       DACDID010000400          501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.413, whole genome shotgun sequence.
ACCESSION   DACDID010000400 DACDID010000000
VERSION     DACDID010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.413"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..>501
                     /locus_tag="IV236_002938"
     CDS             200..>501
                     /locus_tag="IV236_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773057.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLS"
BASE COUNT          197 a           63 c           89 g          152 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct c
//
LOCUS       DACDID010000401          497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.414, whole genome shotgun sequence.
ACCESSION   DACDID010000401 DACDID010000000
VERSION     DACDID010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.414"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          175 a           73 c           98 g          151 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagttta
//
LOCUS       DACDID010000402          490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.415, whole genome shotgun sequence.
ACCESSION   DACDID010000402 DACDID010000000
VERSION     DACDID010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.415"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..347)
                     /locus_tag="IV236_002939"
     CDS             complement(<1..347)
                     /locus_tag="IV236_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8773058.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          148 a           92 c           86 g          164 t
ORIGIN      
        1 atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt
       61 ttcgcagaaa aggtctgccc acaatcatag catagaaaac gggtacgttt taattctaaa
      121 atagtcaact tttctttgac tttcaatatc tgagtacgtg tcgtatagga accgttgcgg
      181 ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa tctcccttta
      241 ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc atcaaaaaat
      301 aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccataga gcgactcctt
      361 tggtagggtg atgttttggt cgactaaatt ttaccaaaag tgagtctctt tttgagttga
      421 aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaacaacac aaaatattat
      481 agaacccttt
//
LOCUS       DACDID010000403          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.416, whole genome shotgun sequence.
ACCESSION   DACDID010000403 DACDID010000000
VERSION     DACDID010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.416"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IV236_002940"
     CDS             complement(61..258)
                     /locus_tag="IV236_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8773059.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDID010000404          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.417, whole genome shotgun sequence.
ACCESSION   DACDID010000404 DACDID010000000
VERSION     DACDID010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.417"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>481)
                     /locus_tag="IV236_002941"
     CDS             complement(<1..>481)
                     /locus_tag="IV236_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8773060.1"
                     /translation="SSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLIIGTG
                     DMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFAD
                     LPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQ
                     "
BASE COUNT          161 a           92 c           53 g          175 t
ORIGIN      
        1 tttgaatatt ataatctgcc tttattaaaa tcgaacgttc tgaatctcga tgagagatat
       61 ctacatttgc aatctctctc gcccattctt ttgccgattg atttaaactt atgaaattgg
      121 tcttatcact gaaagcaact tctgtaggaa ctttctccga aaagaaacac ggtaaatctg
      181 caaattgagc ttcaataccc actaacggaa ccccttcaaa taatgatggc aacattaata
      241 cgtcaaatgc ttgataaaat ttttctatat catttctatt tcctaaaaat ttaatatcat
      301 catctatgcc acttttcttt gcataatttt ttatttcttc ttccatatca ccagttccaa
      361 taattaataa tacggaatta ttcttaatag cgtgaaattc tttaaaaatt tcaatcaaaa
      421 aacgatgatt tttagaatat gaaattcttc caatatgtcc cattacaaac gtctgacttg
      481 a
//
LOCUS       DACDID010000405          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.418, whole genome shotgun sequence.
ACCESSION   DACDID010000405 DACDID010000000
VERSION     DACDID010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.418"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV236_002942"
     CDS             complement(<1..430)
                     /locus_tag="IV236_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8773061.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACDID010000406          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.419, whole genome shotgun sequence.
ACCESSION   DACDID010000406 DACDID010000000
VERSION     DACDID010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.419"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IV236_002943"
     tRNA            complement(20..91)
                     /locus_tag="IV236_002943"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IV236_002944"
     tRNA            complement(113..200)
                     /locus_tag="IV236_002944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IV236_002945"
     tRNA            complement(225..298)
                     /locus_tag="IV236_002945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IV236_002946"
     tRNA            complement(312..382)
                     /locus_tag="IV236_002946"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IV236_002947"
     tRNA            complement(384..459)
                     /locus_tag="IV236_002947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDID010000407          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.420, whole genome shotgun sequence.
ACCESSION   DACDID010000407 DACDID010000000
VERSION     DACDID010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.420"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..283
                     /locus_tag="IV236_002948"
     CDS             89..283
                     /locus_tag="IV236_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773062.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          190 a           57 c           77 g          119 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcg
//
LOCUS       DACDID010000408          439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.421, whole genome shotgun sequence.
ACCESSION   DACDID010000408 DACDID010000000
VERSION     DACDID010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.421"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..>439
                     /locus_tag="IV236_002949"
     CDS             18..>439
                     /locus_tag="IV236_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8773063.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          158 a           83 c           91 g          107 t
ORIGIN      
        1 aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt gtaatgatag
       61 aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt aaacgtggac
      121 gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca cttgaatact
      181 ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt tttcctgctg
      241 aggaaaaaga atacattgaa aaaagatcaa ctgaaggttg gaccccagac gtcattattg
      301 gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg tttaagacgg
      361 gagaattcaa tgttttacat ttaccgatgc aaggaaaacg aaaaccaaat ggttataagg
      421 aaaaacgtgg aaaacaggc
//
LOCUS       DACDID010000409          435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.422, whole genome shotgun sequence.
ACCESSION   DACDID010000409 DACDID010000000
VERSION     DACDID010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.422"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          147 a           70 c           68 g          150 t
ORIGIN      
        1 aagctaattt taagagattc ttattagaag acgtttagga atataactaa gaaaatgatt
       61 atacaggtaa ttgtcatttc aataatgttt tctaattttc gcgtctttgt tttaagaaaa
      121 aggccagaat cagttggaaa aaagttcgta cgatagtgaa gaggggcaaa atttcctact
      181 aatattccca ctaacaagag ataaaacggc caggttggtg tgattcctct ataaaaaaat
      241 gcccatgcta ccgaaatccc atagattaac aactgaacaa tttttacaat gaaaaaattg
      301 ttctgccatt actcaaagtc tcttcctcct tgtatcgttt atttgtttca ttttatcatt
      361 atgataggcg cttgaacaat ccattttgta ttttattgag tactcttgga taaactacca
      421 gcgattatca tattt
//
LOCUS       DACDID010000410          434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.423, whole genome shotgun sequence.
ACCESSION   DACDID010000410 DACDID010000000
VERSION     DACDID010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.423"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>434)
                     /locus_tag="IV236_002950"
     CDS             complement(<1..>434)
                     /locus_tag="IV236_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773064.1"
                     /translation="YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKW
                     YWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSA
                     GFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNN"
BASE COUNT          162 a           77 c           76 g          119 t
ORIGIN      
        1 tagttgttat agttattatt taaaatatgt gtcagcatat cactaggagc cataccactt
       61 aattcttttc ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt
      121 aagaatcccg cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta
      181 gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa
      241 accatagcga cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag
      301 taccattttt ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa
      361 aatgttaaag cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag
      421 gcggaaaatg tata
//
LOCUS       DACDID010000411          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.424, whole genome shotgun sequence.
ACCESSION   DACDID010000411 DACDID010000000
VERSION     DACDID010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.424"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>432)
                     /locus_tag="IV236_002951"
     CDS             complement(<1..>432)
                     /locus_tag="IV236_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8773065.1"
                     /translation="SKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIP
                     VSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFG
                     YGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQ"
BASE COUNT          137 a           81 c           55 g          159 t
ORIGIN      
        1 cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc acatctgtag
       61 tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa tcggtctctc
      121 cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa atcgtaaaac
      181 gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct ttttctggaa
      241 gtggagtttt atctgattca tttgactcta taacttctac ttttctatca atcgatacag
      301 gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat accaatagaa
      361 ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta atgctctttc
      421 taaattttga at
//
LOCUS       DACDID010000412          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.425, whole genome shotgun sequence.
ACCESSION   DACDID010000412 DACDID010000000
VERSION     DACDID010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.425"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..301
                     /locus_tag="IV236_002952"
     CDS             <1..301
                     /locus_tag="IV236_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8773066.1"
                     /translation="ISLKDICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSY
                     KAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            312..>427
                     /locus_tag="IV236_002953"
     CDS             312..>427
                     /locus_tag="IV236_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAP8773067.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSG"
BASE COUNT          160 a           65 c           92 g          110 t
ORIGIN      
        1 cattagtttg aaagatattt gtagagaagt cgcaaaagaa ttaaaagatt gtgaacgctc
       61 gaatggtacg tatgattttg atcgttttta tcaaggaaaa ggggatatta attctcagat
      121 tcttttctct tataaagcag aacaatacta caaaggctat aaaaatcatg aacatgaatt
      181 tgtacctgaa gtgaaaaagg attggaacga tcaattgaag catgagatcc atcaacagga
      241 aattagaaag caaaaacgtg cgatgttgtt tcaacaaggc agacaacaag aaagggagtg
      301 agatcgtcac gatgaaaact gtcattttag cagaaaaacc gtcacaagcg aaggcctatg
      361 ccgatagctt ttctaaagcc acccgaaaag acgggtattt tgaaatccaa gatcgattgt
      421 tttcggg
//
LOCUS       DACDID010000413          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.426, whole genome shotgun sequence.
ACCESSION   DACDID010000413 DACDID010000000
VERSION     DACDID010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.426"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..301
                     /locus_tag="IV236_002954"
     CDS             <1..301
                     /locus_tag="IV236_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8773068.1"
                     /translation="INLKDICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSY
                     KAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            312..>427
                     /locus_tag="IV236_002955"
     CDS             312..>427
                     /locus_tag="IV236_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAP8773069.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSG"
BASE COUNT          162 a           61 c           90 g          114 t
ORIGIN      
        1 tattaatttg aaagatattt gtagagaagt cgcaaaagaa ttaaaagctt gtgaacgcgt
       61 ggatggtacg tacgattttg atcgttttta tcaagaaaaa ggggatatta atgctcagat
      121 tcttttctct tataaagcag aacaatacta caaaggctat aaaaatcatg aacatgaatt
      181 tgtacctgaa gtaaaaaagg attggaatga tcaattgaag catgaaatct accaacaaga
      241 gatcagaaag caaaaacgtg ccatgttgtt tcaacaaggt agacagcaag aaagggagtg
      301 aaatcgtcat gatgaaaacc gttattttag cagaaaaacc gtcacaagcg aaggcttatg
      361 ccgatagctt ttctaaagct acccgaaaag atgggtattt tgagatccaa gatcgattgt
      421 tttcagg
//
LOCUS       DACDID010000414          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.427, whole genome shotgun sequence.
ACCESSION   DACDID010000414 DACDID010000000
VERSION     DACDID010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.427"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(170..415)
                     /locus_tag="IV236_002956"
     CDS             complement(170..415)
                     /locus_tag="IV236_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8773070.1"
                     /translation="MIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          130 a           83 c           58 g          153 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cact
//
LOCUS       DACDID010000415          414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.428, whole genome shotgun sequence.
ACCESSION   DACDID010000415 DACDID010000000
VERSION     DACDID010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.428"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..>414
                     /locus_tag="IV236_002957"
     CDS             66..>414
                     /locus_tag="IV236_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8773071.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          167 a           47 c           85 g          115 t
ORIGIN      
        1 tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt cctaaaaatg
       61 gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac aaagaatcgg
      121 ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt acaagtaaag
      181 attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt acagcttgtg
      241 taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag ggtgtaaata
      301 tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa gcaattgaaa
      361 agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg ccag
//
LOCUS       DACDID010000416          411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.429, whole genome shotgun sequence.
ACCESSION   DACDID010000416 DACDID010000000
VERSION     DACDID010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.429"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>411)
                     /locus_tag="IV236_002958"
     CDS             complement(<1..>411)
                     /locus_tag="IV236_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8773072.1"
                     /translation="NSDILLAIFGHKLGSPVDNYRSGTIAEIDYFYKNSKGEVGVFFL
                     DEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKVNYLIEKAQNNLESK
                     RFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
BASE COUNT          131 a           77 c           48 g          155 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt t
//
LOCUS       DACDID010000417          406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.430, whole genome shotgun sequence.
ACCESSION   DACDID010000417 DACDID010000000
VERSION     DACDID010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.430"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           68 c           64 g          131 t
ORIGIN      
        1 taaactccat ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata
       61 gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga
      121 tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta
      181 gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac
      241 ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca
      301 attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct
      361 gaataaaaaa caaacctgtg catttctagg aatcgataat aatact
//
LOCUS       DACDID010000418          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.431, whole genome shotgun sequence.
ACCESSION   DACDID010000418 DACDID010000000
VERSION     DACDID010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.431"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IV236_002959"
     CDS             <1..>404
                     /locus_tag="IV236_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773073.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDID010000419          403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.432, whole genome shotgun sequence.
ACCESSION   DACDID010000419 DACDID010000000
VERSION     DACDID010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.432"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV236_002960"
     CDS             <1..378
                     /locus_tag="IV236_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8773074.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          153 a           42 c           67 g          141 t
ORIGIN      
        1 ccaatagaat ttgacaatga tgaaagattg ctgctaatat atgcgtttat ggatgaaaat
       61 tctatctttt caactgataa ttcaacttat agaaaagtag ttcaatggga aagtgataac
      121 aatttaagga gttacttgag tgatagagta tttgatgtac tagaaaaatt aagtaaatcg
      181 ggaattttaa aggaagaata cgattcttct tttgatgagt atccatcacg ctatattttg
      241 atttcagagg actataagaa atttaaaagg tattttttaa aaaatgaaaa aaagatatct
      301 gttattatgg agaaatacaa agctccaata caggctgatc cattttccaa tttggatgaa
      361 tcagatttac cgttttaaat tttaaataga taatttcatt ttt
//
LOCUS       DACDID010000420          399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.433, whole genome shotgun sequence.
ACCESSION   DACDID010000420 DACDID010000000
VERSION     DACDID010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.433"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DACDID010000421          388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.434, whole genome shotgun sequence.
ACCESSION   DACDID010000421 DACDID010000000
VERSION     DACDID010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.434"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..122
                     /locus_tag="IV236_002961"
     CDS             <1..122
                     /locus_tag="IV236_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8773075.1"
                     /translation="ANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            123..>388
                     /locus_tag="IV236_002962"
     CDS             123..>388
                     /locus_tag="IV236_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8773076.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKI"
BASE COUNT          148 a           47 c           81 g          112 t
ORIGIN      
        1 ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca
       61 aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat
      121 aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa
      181 atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct
      241 atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt
      301 tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat
      361 tagctacaga tactgtaggg aaaattga
//
LOCUS       DACDID010000422          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.435, whole genome shotgun sequence.
ACCESSION   DACDID010000422 DACDID010000000
VERSION     DACDID010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.435"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IV236_002963"
     tRNA            complement(21..94)
                     /locus_tag="IV236_002963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IV236_002964"
     tRNA            complement(108..181)
                     /locus_tag="IV236_002964"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IV236_002965"
     tRNA            complement(209..281)
                     /locus_tag="IV236_002965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IV236_002966"
     tRNA            complement(301..374)
                     /locus_tag="IV236_002966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACDID010000423          359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.436, whole genome shotgun sequence.
ACCESSION   DACDID010000423 DACDID010000000
VERSION     DACDID010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.436"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>359
                     /locus_tag="IV236_002967"
     CDS             <1..>359
                     /locus_tag="IV236_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8773077.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVT"
BASE COUNT          143 a           67 c           55 g           94 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaac
//
LOCUS       DACDID010000424          355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.437, whole genome shotgun sequence.
ACCESSION   DACDID010000424 DACDID010000000
VERSION     DACDID010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.437"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>355
                     /locus_tag="IV236_002968"
     CDS             <1..>355
                     /locus_tag="IV236_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8773078.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQL"
BASE COUNT          118 a           57 c           67 g          113 t
ORIGIN      
        1 cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc
       61 tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa
      121 aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc
      181 gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc
      241 gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt
      301 cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa ttgga
//
LOCUS       DACDID010000425          353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.438, whole genome shotgun sequence.
ACCESSION   DACDID010000425 DACDID010000000
VERSION     DACDID010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.438"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>353)
                     /locus_tag="IV236_002969"
     CDS             complement(<1..>353)
                     /locus_tag="IV236_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773079.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIP"
BASE COUNT          120 a           58 c           42 g          133 t
ORIGIN      
        1 aggaattttg attttagaat tctgtggagc acctattgga agataataat tcattataat
       61 tttgtagtct tcatgaggaa ataaagtact tttaggattg ctaagatatt ctcgtaccga
      121 atctaagaat ggcttatcca ttgaatcttc atccccattt aaactaaaaa acattcctac
      181 aattgctttt gcaactctaa gtgaattcat tttctttatt gaatgtgtta aatcagactt
      241 accattcttt aaacttgtat tgttataaag gaaattttta ttgtcttctg caaatctaat
      301 atattcctca acatactcac taccaaaaaa actattacat ttactacata acg
//
LOCUS       DACDID010000426          351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.denovo.439, whole genome shotgun sequence.
ACCESSION   DACDID010000426 DACDID010000000
VERSION     DACDID010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.denovo.439"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          117 a           53 c           53 g          128 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaagagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acatttttga
      121 tcctttttat ttgattaatt atatccagct ggataataat tttgggggca acatttctat
      181 cttactatta tcttcctaaa gtagtagact ttcttattgg gaaaaattac aacaaaaagg
      241 agtggcttag ttgacaactc ctttttgttt acaaatcaat tggatatcta taaattgtat
      301 tttctaacga attttataga catctgattt gtcaaattaa gctagacaat a
//
LOCUS       DACDID010000427        10003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.096, whole genome shotgun sequence.
ACCESSION   DACDID010000427 DACDID010000000
VERSION     DACDID010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.096"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(163..1035)
                     /locus_tag="IV236_002970"
     CDS             complement(163..1035)
                     /locus_tag="IV236_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8773080.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1163..1732
                     /locus_tag="IV236_002971"
     CDS             1163..1732
                     /locus_tag="IV236_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8773081.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1799..2536
                     /locus_tag="IV236_002972"
     CDS             1799..2536
                     /locus_tag="IV236_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8773082.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2767..4035
                     /locus_tag="IV236_002973"
     CDS             2767..4035
                     /locus_tag="IV236_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8773083.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4522..4986
                     /locus_tag="IV236_002974"
     CDS             4522..4986
                     /locus_tag="IV236_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8773084.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4970..5599
                     /locus_tag="IV236_002975"
     CDS             4970..5599
                     /locus_tag="IV236_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8773085.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5953..7431
                     /gene="msr"
                     /locus_tag="IV236_002976"
     CDS             5953..7431
                     /gene="msr"
                     /locus_tag="IV236_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8773086.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7478..7924
                     /locus_tag="IV236_002977"
     CDS             7478..7924
                     /locus_tag="IV236_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8773087.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8123..8638)
                     /locus_tag="IV236_002978"
     CDS             complement(8123..8638)
                     /locus_tag="IV236_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8773088.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8755..9957
                     /locus_tag="IV236_002979"
     CDS             8755..9957
                     /locus_tag="IV236_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8773089.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3190 a         1750 c         2076 g         2987 t
ORIGIN      
        1 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
       61 aaaggaagga acaaattaag aaacttatgt tatttaatca ttggatcttt tgcttaactt
      121 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
      181 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
      241 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat actttttccg tcccataagt
      301 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
      361 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
      421 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
      481 ttcctgcgga tggatgactg tttgctttgc taaaggatga ttccgatgta tcaaaagtcc
      541 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
      601 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
      661 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
      721 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
      781 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
      841 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagcctcgag tgaaaagtgt
      901 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
      961 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
     1021 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
     1081 aaaggaatag ttaagtatgt tttataagta ttagacatca taagccgtcc gttataaagt
     1141 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
     1201 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagcgg aaaacgaaga
     1261 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
     1321 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
     1381 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
     1441 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
     1501 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
     1561 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
     1621 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
     1681 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
     1741 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
     1801 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
     1861 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
     1921 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
     1981 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
     2041 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
     2101 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
     2161 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
     2221 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
     2281 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
     2341 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
     2401 tgaacttcat gaagcgcaaa aagaatgggg attaacatct gaagatatag cttctgctgt
     2461 agcatttgcg atagaaacgc cagaccggat gtctgtcagc aacatgatta tccgtccgac
     2521 gacacaggaa gtataatcca taacggataa tatccagaga gaattacttg ttaaatagtt
     2581 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
     2641 ttatgcagaa aaagcaaatt tattaaaaag aaacaaatgt tatttaaata tggacattga
     2701 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
     2761 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
     2821 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
     2881 tcttcagaaa caggaattga aaacgataca gaaagtactt tgcagtcagc tactgtctca
     2941 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
     3001 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
     3061 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
     3121 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
     3181 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
     3241 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
     3301 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
     3361 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
     3421 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
     3481 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
     3541 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
     3601 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
     3661 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
     3721 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
     3781 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
     3841 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
     3901 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
     3961 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
     4021 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
     4081 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
     4141 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
     4201 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
     4261 tcggaaattt ttggcggtgt aaaattatct actgcagaag agctatcttc tatagtttga
     4321 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
     4381 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
     4441 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
     4501 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
     4561 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
     4621 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
     4681 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
     4741 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
     4801 gaaaaagccc agaagaaaat caagcattgt gaaatggacc ataaagatac aggttccgtg
     4861 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
     4921 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
     4981 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
     5041 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
     5101 tgtcaatatg aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
     5161 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
     5221 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag cttacgaatc
     5281 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
     5341 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
     5401 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
     5461 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
     5521 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
     5581 gatcactatg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
     5641 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
     5701 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
     5761 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
     5821 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
     5881 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
     5941 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
     6001 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
     6061 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
     6121 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
     6181 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
     6241 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
     6301 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
     6361 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
     6421 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
     6481 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
     6541 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
     6601 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
     6661 caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
     6721 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
     6781 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
     6841 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
     6901 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
     6961 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
     7021 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
     7081 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
     7141 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
     7201 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
     7261 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
     7321 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
     7381 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
     7441 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
     7501 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
     7561 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
     7621 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
     7681 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
     7741 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
     7801 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
     7861 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
     7921 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
     7981 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
     8041 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
     8101 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
     8161 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
     8221 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
     8281 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
     8341 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
     8401 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
     8461 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
     8521 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
     8581 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
     8641 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
     8701 tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
     8761 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
     8821 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
     8881 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
     8941 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
     9001 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
     9061 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
     9121 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
     9181 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
     9241 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
     9301 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
     9361 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
     9421 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
     9481 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
     9541 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
     9601 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
     9661 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
     9721 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
     9781 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
     9841 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
     9901 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
     9961 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACDID010000428         7618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.124, whole genome shotgun sequence.
ACCESSION   DACDID010000428 DACDID010000000
VERSION     DACDID010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.124"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..713
                     /locus_tag="IV236_002980"
     CDS             <1..713
                     /locus_tag="IV236_002980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8773090.1"
                     /translation="IWDEWAFERYIKSPDYNGPDMTDFGHRCLTDPEFNEEYKKQSKL
                     FCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIV
                     SAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLI
                     AHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMED
                     IQLEGYDPHPAIKAPIAV"
     gene            733..1260
                     /locus_tag="IV236_002981"
     CDS             733..1260
                     /locus_tag="IV236_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8773091.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1323..1976)
                     /locus_tag="IV236_002982"
     CDS             complement(1323..1976)
                     /locus_tag="IV236_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8773092.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2108..2950
                     /locus_tag="IV236_002983"
     CDS             2108..2950
                     /locus_tag="IV236_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8773093.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3108..4004
                     /locus_tag="IV236_002984"
     CDS             3108..4004
                     /locus_tag="IV236_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8773094.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            4007..4720
                     /locus_tag="IV236_002985"
     CDS             4007..4720
                     /locus_tag="IV236_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8773095.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            4740..5258
                     /gene="msrA"
                     /locus_tag="IV236_002986"
     CDS             4740..5258
                     /gene="msrA"
                     /locus_tag="IV236_002986"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8773096.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            5255..5482
                     /locus_tag="IV236_002987"
     CDS             5255..5482
                     /locus_tag="IV236_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8773097.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            5634..6185
                     /gene="lepB"
                     /locus_tag="IV236_002988"
     CDS             5634..6185
                     /gene="lepB"
                     /locus_tag="IV236_002988"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8773098.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(6268..6810)
                     /locus_tag="IV236_002989"
     CDS             complement(6268..6810)
                     /locus_tag="IV236_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8773099.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(7047..7445)
                     /locus_tag="IV236_002990"
     CDS             complement(7047..7445)
                     /locus_tag="IV236_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8773100.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         2715 a         1351 c         1442 g         2110 t
ORIGIN      
        1 atatttggga tgaatgggca tttgaacgct acatcaaaag tccagactat aatggaccag
       61 atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa gaatataaaa
      121 agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct gaaacatacg
      181 gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct
      241 ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat tctagaagat
      301 tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct ccttgtcata
      361 cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat caacgcagtg
      421 gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa
      481 ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga gatgctcatc
      541 tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata cgcgcatttc
      601 cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg gaagatatcc
      661 aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt tagaatcaga
      721 aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt gattggaaaa
      781 gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca aatgacagaa
      841 gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg
      901 aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg cgtgcatgtc
      961 atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat ctttttcatc
     1021 gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt ataccgtaca
     1081 gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg gtctgattgg
     1141 tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt
     1201 gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt gacgatataa
     1261 aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct ttattttttt
     1321 tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat aacaaacaaa
     1381 tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg
     1441 taagaaagtc cgcctgctac catcaaaata acaccagtca ttccaagaga gtgataaaga
     1501 ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt agatactttt
     1561 gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag aatcgcaaac
     1621 aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag caaacaaaaa
     1681 ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac ttggaacacc
     1741 ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa taaaagaatc
     1801 atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa gtgggcacct
     1861 tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc atgggtgact
     1921 gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt ttccatgaaa
     1981 acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta tactaaaaat
     2041 agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga aagagagaga
     2101 gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg aaaaaaatgt
     2161 acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg gaacagtata
     2221 tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga aagcaaaaaa
     2281 tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg atgagctagg
     2341 agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg gaacagtaga
     2401 agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg acagtgatac
     2461 cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa aagaaggtaa
     2521 aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa aattatacat
     2581 cggaatatcc acactagata acttggtaaa aggtggaaga atcagccgaa caactggttt
     2641 gctgtctagt gttttgaaca tacgcgtggt gatgaatttc gcccattctg aattaatccc
     2701 tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg tttaataatt tgaaaaatga
     2761 actgaaagaa ttgccaaatg tgcgacaaat cggtatttca catgcggatg caaaagaatt
     2821 ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg gacatgcata tccctgtact
     2881 gcatacgaat ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa tcatgtatta
     2941 tgtagattag tcgaaaagag gataagccct cagcctgaaa ggcttgcagc ttattctctt
     3001 tttctttata aaattaggaa aataattgtt ttcttttttc ataaagacta cctttttgat
     3061 aaaatttttg ataacattag aaccaatcga atacaagagg tgaaaaaatg aaattaacga
     3121 aacgacattt gatcctcgtt ttgttcccat tattaattgc gatcatcatc tatgcaggtt
     3181 tagctgcttc tataccgaaa gctggcccac taatgaaacc agtaactact gtgagagaag
     3241 atccaaataa aaaagaaaaa cttcactaca tagctatcgg agattcgctg actgaaggag
     3301 taggagatca aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat ctaaaagacc
     3361 gttataattt gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa cgaagtgacc
     3421 agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa acagctgatt
     3481 ttatcacact gactgttggc ggtaatgatt tgatgaaagt tatccaaaat aatttattcg
     3541 ggttaacggt caattccttt aaaaagccac tgaaaaacta tcaggaaaat gtcacagaac
     3601 tattggaaga atttcgatcc ctcaataaaa ccgcacctat ttacatttta ggcatctaca
     3661 atccatttta tttgaacttt cctgagatca ctgacatgca gacaattgtt aacaattgga
     3721 acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg ctactttatc ccaatcaatg
     3781 atttgcttta tcaaggattg aatcaggaag taggtgtcac agaagaagga acagatcaaa
     3841 caactagcac gacagaaaat ctaaatggca tcaaaaataa tgtgctctat gaagaagatc
     3901 atttccatcc aaataattta ggttatcaat tgatggcaaa tgcagtgaga gatcagctga
     3961 tcgagacaca aaaccaatgg ttagagaagg aggatgatca ataaacatga aaacaagatc
     4021 tgacgtatct tcaaacaaaa caaagaaaac aaattactgg aaattcgcct tccttatcct
     4081 tcttggggta gtacttggtt cgaccgtatt tttaggcagc cgtatttttg ctaatcggga
     4141 accagaactc ccagaaatac cagctcttac ggaaagacaa ggagatcctg tcttaacgat
     4201 caattcaaat aaagaaaaag tcaatcaaat catcagcttt ttccttagtg agtatcaaaa
     4261 agatagtgat attgaatata aattttattt ggaaaatgaa gcactactca atggtacttt
     4321 cgaagtacta ggttttccta tcgatttcta tttatatttt gatccgtatg tgatggaaaa
     4381 cggaaatgtc caactaaaag ctagaagtct ttctatcgga acattgagtt tgccaatcaa
     4441 agatgtgatg aatatgatca agagaaacta taaattacca gattggatcg aagtcaatac
     4501 agatgatctg actatcatgc ttaggttgga tcaattccga atgcaaaacg ggatgtatat
     4561 taaagcagat aaaatcgact tagtaaatga tgacattcgg tttagtctat atttgccaaa
     4621 agatacctct gatgcttcaa acaactcaaa taccaacaaa actagcacca gcagtactga
     4681 aagcaagaca aaagaaaata aaaaatcatc ggatgagtag tattagaggg ggaatcataa
     4741 tggaacgagc aatatttgca ggaggctgct tctggtgtat gatccagcct ttcgacacgc
     4801 ttcctggtat ccatacagtg atgtcaggat atactggagg gcatgtacca gatccgactt
     4861 atgaacaagt aaaaagccaa acgactggac atacagaagc agtggagatt atctatgatc
     4921 caacattgat ttcatatgaa caattagtag aactttactg gcagcaaact gatccaactg
     4981 acgctttcgg acaattcgag gatcgcggtg acaattatcg cccggtgatc ttttatttga
     5041 cagaagaaca gcggatcatt gctgaaaaaa gtaagaaaca gttagctgaa agtggacgtt
     5101 tcgaacagcc aatcgttaca aaaatcgaac cagcccaacc attttatcta gcagaagaag
     5161 accatcaaga gtattataaa aagcatcctg aaaattttaa aagaaaccat gcacgccggg
     5221 ctgcctttat cgaagaaaat tggaggaacg aagggtgaat cgaagctttt ttcattattt
     5281 aatgacactg cgaggcggtg gcacacatga tgctttatct gttttcgcta cagaagccgg
     5341 taaagacatc caatttccta agcacagctc tagctacgaa gaaatttcta attatctgga
     5401 actgaatgtc gactatttgc cgtccatgga tatctttgac gaagcatggg aaaaatattt
     5461 agaaaataat catgacatat aagagatagt tttgtacttt atgatcaaaa aagttttttg
     5521 tccaatagcc taattgaaag aattattttt atttcagcta tctagtcgca tttttttgat
     5581 atttttctta taataaagaa agaatgaaaa aaagagggat ggattgatag agattgacaa
     5641 aaaaacaacg ctatattaat cgcttctggc ttgtattcaa atacttactt gtttctgtat
     5701 tccttgcgtt catgttgcgg ggatttttat tcatcccggt accagtggaa ggcaattcaa
     5761 tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg gagatcagac
     5821 gatttgatat tgttgttttt caattagcag atggcacgat ttatatcaaa cgagtaatcg
     5881 gtcttccagg agaaaatgtc agctaccaaa atgatcaatt gaagatcaat gggaaagtag
     5941 ttaaggaacc ttatctaact aaaaacctga aatcagatca tgcaaatgca tcctatacga
     6001 cggactttac actacaagag ctaacgggac aatccaagct tccggaagat aattattttg
     6061 tacttggaga taatcgaaga gtttcaaaag atagtcgttc gtttggcacg atcaataaaa
     6121 ctgatattct aggtaaagca agatttgtct attatccact tgatgaaata aaatggatcc
     6181 agtgagaaac aataaagcgt gcagtctgtt gagactgcac gctttattgt ttaatccaaa
     6241 atcagtttac taccttagta cgtaaaatca aacagctcgt ttaaacactt ttctagcctc
     6301 taaagttttg ataaatgaat aaacaaccat tatttgaata gggatttgga tcatattcct
     6361 gatgaatcta cctagaaaaa ttactttgaa atctgcacca aacaaaacag acatcagcca
     6421 agaattgata aaaaggctga ctgggatcat aatcaataat tgggcagcga tgacggaagg
     6481 caggtttatc tttttatgat ataaaaataa gccattaata aatccaccaa ggaaagccgt
     6541 gataagaaaa gcagcttgaa acgttcctga accaaatatc gtgaaattta aagtgttcga
     6601 caacatttca agcaaaccag cccagatcgg tccatataga taacccataa gcgccatact
     6661 taaaaatccg aatgtgattt gaaaataagc aggaccaatc gctatcttgt ataagatgac
     6721 atcaagggct aataaaagag cagaggtcga caacaatctt gcattcattt cgggcaatct
     6781 aaaaacgtct tttgcaatca aagtgttcaa aatattctct cctttgtcag ttaataataa
     6841 aaggatacca taaaatcacc aatgtgtcat gacacattgg tgatttacat acactggcat
     6901 ctaacaatta aaaaaagggt gagaagactg cttttataaa ctgtaaaaaa gagattgtac
     6961 gaataaaaaa aatagaggcg gagaatactt atcgtatcat ccgcctttgt ttttttgtta
     7021 taaaaaacgt cagattagtt atctgcttac atcttcatcc atacgggttc taatgtattt
     7081 ccgtctagat cttggacttc taatgaaaac atttcttctt caggcattcc catatctact
     7141 tcgtggaaag taccaccatt ttcttttgca atttgagcaa atttttttac ttcatccgcg
     7201 ttttcaacac taaacgaaat caaagcacca cttgtttttt tattatcagc tatttcttta
     7261 tcttttataa attttttata aaaatcatga gagagaagca ttatccaaaa agtgtcatcc
     7321 cataccattg aaccagcttc ttcagtagaa aattcttcat tttttttaaa acccagcttt
     7381 tcataaaaag caacggaacg attgacatca gcaacaggaa aattaacaaa aaccatacta
     7441 ttcatttgtt tcgtctcctt tctgttttgt tcgtctatca tctcttattt aggaaacgct
     7501 gtcaaaaatt tagattcata tatagaaacc aaaataaaaa tttgcatcat caaaaaagtg
     7561 aagatgtgat agaatgagga ctggttcact ttcacttaac taatagatgg aatttctt
//
LOCUS       DACDID010000429         5984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.142, whole genome shotgun sequence.
ACCESSION   DACDID010000429 DACDID010000000
VERSION     DACDID010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.142"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..1027)
                     /locus_tag="IV236_002991"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="IV236_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="IV236_002992"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="IV236_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8773101.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="IV236_002993"
     CDS             1852..3006
                     /locus_tag="IV236_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8773102.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="IV236_002994"
     CDS             3221..4189
                     /locus_tag="IV236_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8773103.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="IV236_002995"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="IV236_002995"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8773104.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="IV236_002996"
     CDS             5219..5827
                     /locus_tag="IV236_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8773105.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1159 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatc
//
LOCUS       DACDID010000430         5967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.143, whole genome shotgun sequence.
ACCESSION   DACDID010000430 DACDID010000000
VERSION     DACDID010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.143"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            213..1109
                     /locus_tag="IV236_002997"
     CDS             213..1109
                     /locus_tag="IV236_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8773106.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1259..2200)
                     /locus_tag="IV236_002998"
     CDS             complement(1259..2200)
                     /locus_tag="IV236_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8773107.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2326..3447
                     /locus_tag="IV236_002999"
     CDS             2326..3447
                     /locus_tag="IV236_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8773108.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3705..5207
                     /gene="lsa"
                     /locus_tag="IV236_003000"
     CDS             3705..5207
                     /gene="lsa"
                     /locus_tag="IV236_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8773109.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSNDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5274..5801
                     /locus_tag="IV236_003001"
     CDS             5274..5801
                     /locus_tag="IV236_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8773110.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1968 a         1042 c         1236 g         1721 t
ORIGIN      
        1 atatttaaca atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt
       61 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
      121 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
      181 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
      241 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
      301 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
      361 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
      421 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
      481 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
      541 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
      601 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
      661 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
      721 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
      781 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
      841 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
      901 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
      961 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
     1021 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     1081 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     1141 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     1201 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     1261 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     1321 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     1381 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     1441 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     1501 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     1561 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     1621 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     1681 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     1741 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     1801 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     1861 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     1921 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     1981 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     2041 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     2101 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     2161 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     2221 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     2281 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     2341 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     2401 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     2461 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     2521 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     2581 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     2641 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     2701 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     2761 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     2821 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     2881 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     2941 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     3001 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     3061 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     3121 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     3181 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     3241 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     3301 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     3361 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     3421 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     3481 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     3541 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     3601 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     3661 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     3721 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     3781 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     3841 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     3901 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     3961 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     4021 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     4081 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     4141 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     4201 tcatcgtggt cagcaatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     4261 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     4321 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     4381 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     4441 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     4501 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     4561 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     4621 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     4681 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     4741 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     4801 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     4861 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     4921 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     4981 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     5041 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     5101 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     5161 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     5221 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     5281 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     5341 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     5401 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     5461 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     5521 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     5581 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     5641 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     5701 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     5761 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     5821 aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat
     5881 tcttttgata atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat
     5941 tgaaattttg tttctttaga tatatac
//
LOCUS       DACDID010000431         5967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.144, whole genome shotgun sequence.
ACCESSION   DACDID010000431 DACDID010000000
VERSION     DACDID010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.144"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            213..1109
                     /locus_tag="IV236_003002"
     CDS             213..1109
                     /locus_tag="IV236_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8773111.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1259..2200)
                     /locus_tag="IV236_003003"
     CDS             complement(1259..2200)
                     /locus_tag="IV236_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8773112.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2326..3447
                     /locus_tag="IV236_003004"
     CDS             2326..3447
                     /locus_tag="IV236_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8773113.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3705..5207
                     /locus_tag="IV236_003005"
     CDS             3705..5207
                     /locus_tag="IV236_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8773114.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5274..5801
                     /locus_tag="IV236_003006"
     CDS             5274..5801
                     /locus_tag="IV236_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8773115.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1967 a         1043 c         1236 g         1721 t
ORIGIN      
        1 atatttaaca atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt
       61 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
      121 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
      181 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
      241 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
      301 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
      361 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
      421 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
      481 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
      541 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
      601 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
      661 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
      721 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
      781 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
      841 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
      901 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
      961 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
     1021 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     1081 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     1141 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     1201 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     1261 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     1321 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     1381 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     1441 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     1501 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     1561 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     1621 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     1681 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     1741 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     1801 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     1861 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     1921 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     1981 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     2041 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     2101 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     2161 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     2221 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     2281 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     2341 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     2401 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     2461 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     2521 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     2581 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     2641 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     2701 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     2761 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     2821 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     2881 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     2941 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     3001 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     3061 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     3121 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     3181 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     3241 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     3301 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     3361 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     3421 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     3481 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     3541 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     3601 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     3661 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     3721 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     3781 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     3841 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     3901 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     3961 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     4021 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     4081 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     4141 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     4201 tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     4261 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     4321 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     4381 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     4441 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     4501 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     4561 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     4621 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     4681 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     4741 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     4801 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     4861 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     4921 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     4981 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     5041 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     5101 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     5161 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     5221 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     5281 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     5341 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     5401 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     5461 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     5521 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     5581 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     5641 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     5701 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     5761 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     5821 aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat
     5881 tcttttgata atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat
     5941 tgaaattttg tttctttaga tatatac
//
LOCUS       DACDID010000432         4117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.179, whole genome shotgun sequence.
ACCESSION   DACDID010000432 DACDID010000000
VERSION     DACDID010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.179"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..544
                     /gene="dfrG"
                     /locus_tag="IV236_003007"
     CDS             47..544
                     /gene="dfrG"
                     /locus_tag="IV236_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8773116.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(616..2568)
                     /locus_tag="IV236_003008"
     CDS             complement(616..2568)
                     /locus_tag="IV236_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8773117.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2575..2871)
                     /locus_tag="IV236_003009"
     CDS             complement(2575..2871)
                     /locus_tag="IV236_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8773118.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3046..3849)
                     /locus_tag="IV236_003010"
     CDS             complement(3046..3849)
                     /locus_tag="IV236_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8773119.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(3865..3981)
                     /locus_tag="IV236_003011"
     CDS             complement(3865..3981)
                     /locus_tag="IV236_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8773120.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1249 a          755 c          657 g         1456 t
ORIGIN      
        1 aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga aagtttcttt
       61 gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc cttggaggat
      121 tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa tattaggtag
      181 gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta ttctgacgag
      241 agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag atgtttttga
      301 gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt ataatttgtt
      361 tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg aaggagatac
      421 tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa aagggataaa
      481 gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa acttattgag
      541 ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc ttgggattac
      601 tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt gtttatctgt
      661 aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca
      721 aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg gattgaagtt
      781 agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt caagagatga
      841 tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa catttaatag
      901 catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat caactgaata
      961 aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat atttgcgata
     1021 tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta gtaattcagt
     1081 tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga tatctcccca
     1141 atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc tgcttttcgt
     1201 cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt tcgtgaatac
     1261 tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc attcgtcggc
     1321 attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag catcaacatt
     1381 taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa gataagcatt
     1441 cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat ctaacctatc
     1501 atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat cttcatttaa
     1561 ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat atcttgataa
     1621 gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc ccgtatattt
     1681 ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct tgtccgtatt
     1741 aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc gttctaaaaa
     1801 ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa ctccaattcc
     1861 taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag cgaacattgg
     1921 aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg gtagaacatt
     1981 agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac ttttccccct
     2041 tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc agccttcttt
     2101 atgaaattga taatctggct caacatcttg gatgctattg atttctataa ggcgtttaat
     2161 ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg atacacctct
     2221 gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg aactaccagt
     2281 cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt ctttcccaaa
     2341 attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag aaccgttcca
     2401 tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc gttttggagt
     2461 taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg gaattttaac
     2521 atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg tctatcaacc
     2581 cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct tgttggcaat
     2641 ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca ctgcttcgct
     2701 ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt gctctgatat
     2761 ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca taggaaaatg
     2821 cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca ttcctacaat
     2881 aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg tgtaaattaa
     2941 caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa tttgatactc
     3001 atttaggttt acattttcac tttttgtact ccaaaactga actacttaat atgcaagacg
     3061 ttgtcttttt tttgtatatt caattttgaa aacatttccg caaagttcag acggacctcg
     3121 atgtgttgat gaataaaatt tattagtaat aacttctata aggttatcaa tccctatatt
     3181 actttttgca cttccatgat aaagagggaa cagagaacaa ttctgaaatc ttatgctttc
     3241 ctcttgttcg agttccaatg cttctaatga tttaccggac atatatttct ctaaaaggtc
     3301 atcgtttccc tctattaccg tatcccattg ttcagattcg gtaaagttcg tcacacacat
     3361 attaggatac agttctacct tctgtttgat tacaatttcg gcagaaagtt tctctttaat
     3421 atcctgataa accgttgata aatcaattcc attttggtca atcttattga taaaaaagat
     3481 tgtgggaatc cccattttcc taagtgcatg aaataatata cgagtttgtg cttgtacgcc
     3541 atcttttgca gaaatcagta gaattgcccc atctaaaact gataatgaac gatatacttc
     3601 tgctaagaaa tccatatgtc ctggcgtgtc tatgatgttc accttcgtat tttcccactg
     3661 aaaagaggtt attcctgtct gaattgtaat tcctctctga cgttctaaaa gcgtattatc
     3721 cgtcctcgtt gtacctttgt ccacgcttcc taattctgta atcgctccac tgttatataa
     3781 taagctttct gttaaggtag tttttcctgc atcaacatga gctaaaactc caatattaat
     3841 aattttcatg tgattttcct ccattcaaaa acccaaaagg gcataaaaat cccagtgata
     3901 aatactttta tcactgggat ttttatgcat aaccataggt atacaaagca tacagatatt
     3961 ctctggatac tttagaatca catgataaag gtattcttaa actgggtaca aaaaactaag
     4021 ccctcctaaa aaaggacatc taattatttg ttcccgctat caaattgaca gtttatttaa
     4081 gaataccttg ccgcatattt attaactcct tttaaat
//
LOCUS       DACDID010000433         1770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.267, whole genome shotgun sequence.
ACCESSION   DACDID010000433 DACDID010000000
VERSION     DACDID010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.267"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            81..863
                     /locus_tag="IV236_003012"
     CDS             81..863
                     /locus_tag="IV236_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8773121.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(989..1720)
                     /gene="erm(A)"
                     /locus_tag="IV236_003013"
     CDS             complement(989..1720)
                     /gene="erm(A)"
                     /locus_tag="IV236_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8773122.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          570 a          270 c          327 g          603 t
ORIGIN      
        1 ggtatatgtc caatctaggg taagtaaatt gagtatcaat ataaacttta tatgaacata
       61 atcagttacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca aatcaagcga
      121 ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga gtatatctat
      181 ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt ctagtcgtcg
      241 tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta atgactatat
      301 caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt ataaatagga
      361 gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt gagtggctca
      421 ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat ttggctattg
      481 ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct tcaagtatac
      541 ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg ccagaactaa
      601 ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga atgtggcaaa
      661 cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct atacctcttt
      721 tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg ggagagtgtg
      781 atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat atgaaaaatt
      841 ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct tacttttaaa
      901 actactgatt tattgataaa tattgaacaa tttttgggaa gaataaagcg tcctcttgtg
      961 aaattagaga acgctttatt actttaattt agtgaaacaa tttgtaacta ttgaaaatag
     1021 aaagaaattg ttccttcgat agtttattaa tattagtgac atttgcatgc ttcaaagcct
     1081 gtcggaattg gtttttagtg aaaagaacac gatattcacg gtttacccac ttataaacaa
     1141 aagatcgata ctttttgtag tccttctttg aaatcaatgg ttgatgtcgt tcaagaacaa
     1201 tcaatacaga gtctacactt ggcttaggat gaaaatatag tggtggtact tttttgagca
     1261 tttttatatc catctccacc attaatagta aacccaaagc tcgttgcaga ttttgcaatc
     1321 ttttcgcaaa tcccttctca acgataagat agctatattt agcctgactt tcaaaggtaa
     1381 ttcttttgac aatatccgta ctgatgttat aaggaatatt accatatatc ttatagttta
     1441 tatgttttgg gaaggaaaat tttagaatat ccgtttgaat cacttttata ttctcagagg
     1501 ggtttaccgc ttctttagtc acttgacata agcctccatc aatttctata gcagtaactg
     1561 atcgactcat tttgactagc tctttggtaa aatgtccttt tcctgatccg atttctatta
     1621 cgttgtcttg tttactgata ttcgtgtgat tcaatatttc ttttacatgc tttttagaag
     1681 taataaaatt ttgcgtgtct ttagggtttt tctggttcat tataaccttc tccttactgg
     1741 ttataatgaa ctgacattaa gcgttcatta
//
LOCUS       DACDID010000434         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.288, whole genome shotgun sequence.
ACCESSION   DACDID010000434 DACDID010000000
VERSION     DACDID010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.288"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IV236_003014"
                     /pseudo
     CDS             262..373
                     /locus_tag="IV236_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IV236_003015"
     CDS             394..477
                     /locus_tag="IV236_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8773123.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IV236_003016"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IV236_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8773124.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDID010000435         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.308, whole genome shotgun sequence.
ACCESSION   DACDID010000435 DACDID010000000
VERSION     DACDID010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.308"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV236_003017"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV236_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV236_003018"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV236_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8773125.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDID010000436         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.327, whole genome shotgun sequence.
ACCESSION   DACDID010000436 DACDID010000000
VERSION     DACDID010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.327"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="IV236_003019"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="IV236_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8773126.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DACDID010000437         1093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.336, whole genome shotgun sequence.
ACCESSION   DACDID010000437 DACDID010000000
VERSION     DACDID010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.336"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..>1093
                     /locus_tag="IV236_003020"
     CDS             63..>1093
                     /locus_tag="IV236_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8773127.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFN"
BASE COUNT          364 a          173 c          223 g          333 t
ORIGIN      
        1 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
       61 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
      121 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
      181 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
      241 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
      301 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
      361 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
      421 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
      481 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
      541 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat
      601 ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga
      661 atgtgacgga ttgtaaaatc tgttttaagt acggtttata ctatagccct gttagtactc
      721 cagcagattt tcggatgctt actcctattg tactggagca agcctttaga aaagctggaa
      781 cagaattgtt agagccatat cttagtttta aagtttatgc accacaggaa tatctttcac
      841 gggcatataa cgatgctccc aaatattgtg caaatatcgt aaatactcaa ctgaaaaata
      901 atgaggtcat tattattgga gaaattcctg ctcgatgtat tcaagattat cgcaatgatt
      961 taactttttt tacaaatggg cttagtgttt gtttagcaga gctaaaagga tatcaggtta
     1021 ccactggcga acctgtttgc cagacccgtc gtctaaatag tcggatagat aaagtaagat
     1081 atatgttcaa taa
//
LOCUS       DACDID010000438          882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.365, whole genome shotgun sequence.
ACCESSION   DACDID010000438 DACDID010000000
VERSION     DACDID010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.365"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            167..715
                     /gene="aac(6')"
                     /locus_tag="IV236_003021"
     CDS             167..715
                     /gene="aac(6')"
                     /locus_tag="IV236_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8773128.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          130 c          192 g          248 t
ORIGIN      
        1 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
       61 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      121 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      181 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      241 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      301 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      361 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      421 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      481 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      541 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      601 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      661 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      721 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
      781 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
      841 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aa
//
LOCUS       DACDID010000439          708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20602_2016
            SAMN12640386-rid9888023.guided.387, whole genome shotgun sequence.
ACCESSION   DACDID010000439 DACDID010000000
VERSION     DACDID010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640386
            Sequence Read Archive: SRR10040970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742914.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:19:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,902
            CDSs (with protein)               :: 2,902
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 25 of 64
            Pseudo Genes (incomplete)         :: 29 of 64
            Pseudo Genes (internal stop)      :: 19 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640386-rid9888023.guided.387"
                     /isolate="E20602_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>708
                     /locus_tag="IV236_003022"
     CDS             <1..>708
                     /locus_tag="IV236_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8773129.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYI"
BASE COUNT          297 a           63 c          117 g          231 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc gaatcattaa aaaaacatta
       61 tacagagcct tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat
      121 tggatatgga caaatatata aaatgtatga tgagttatat actgattatc attatccaaa
      181 aactgatgag atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa
      241 aggaattggt acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc
      301 taatgcagtt attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa
      361 atctggtttt agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga
      421 agattgttat ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa
      481 atatttaatt gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag
      541 tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag
      601 tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa
      661 tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattag
//
